不同光质对灵芝生长生理和有效成分的影响及灵芝抗氧化酶的开发研究
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摘要
以飞船搭载的灵芝菌株为材料,研究不同光质对灵芝形态、灵芝多糖含量、抗氧化酶活性的效应,找出适于灵芝生长的最佳光质,为光质选择提供必要依据。
对栽培灵芝进行不同光质处理,在灵芝不同生长发育时期观测其形态变化并测定抗氧化酶体系中主要酶活性。蓝色光质处理在现蕾期、开伞期、弹孢后期多糖含量均高于对照,且差异显著。红色光质处理孢子粉产量最高。蓝色光质处理POD、CAT活性均高于对照。蓝色光质处理提高了抗氧化酶的活性,对灵芝生长有利,表现为保护效应；同时蓝色光质处理可溶性蛋白含量在生长各时期均高于对照,在灵芝菌盖形成期可溶性蛋白含量最大,在开伞期可溶性蛋白质条带上Rf0.18和0.25处有其特征谱带。
研究灵芝液体培养过程中pH值、菌丝体干重、SOD活性、蛋白质含量和灵芝多糖的变化趋势。在灵芝发酵过程中,灵芝菌丝体SOD比活力高于发酵液。菌丝体SOD比活力最大为99.36 U/mg,是发酵液SOD比活力的5.71倍。通过单因素实验确定产SOD适宜的培养基组成,淀粉为碳源时,灵芝发酵液产发SOD活力最高,SOD比活力为206.54 U/mg。以干酪素为氮源时,灵芝发酵液产发SOD活力最高,SOD比活力为228.47 U/mg。研究灵芝发酵液体外抗氧化作用。灵芝乙醇提取物有较好的清除羟基自由基能力,当提取物浓度达1 mg/ml时,清除率达90.06%,EC5o值为0.26 mg/ml。研究表明总酚含量与灵芝乙醇提取物清除羟基自由基能力密切相关。灵芝乙醇提取物总黄酮含量为11.82 mg/g,与还原能力呈正相关。
In order to provide theoretical basis for cultivation,the effects of different light qualities on the form and anti-oxidative enzyme activities of Ganoderma lucidum ever carried by the spacecraft of China were studied. G.lncidum was planted under different light qualities. Their forms were observed and the activity of antioxidant enzyme system was studied. The content of polysaccharides was higher than CK under blue light in the period of budding, parachute phase, after spore ejection. And the highest yield of spores were got under red light. The blue light induced a increase on the content of MDA,the activities of POD,CAT in the end of growth. Antioxidant enzymes are enhanced by blue light,which presents protective efficacy on G.lucidum. The content of protein under the blue light was higher than CK in the period of growth. There were two different spots under blue light in the electrophoretograms of Glucidum.
With Glucidum as materials, the change tendencies of pH,the dry weight of mycelium,SOD,protein and polysaccharides content were investigated during shake flath cultivation of G.lucidum.The results showed that the G.lucidum had a high SOD activity in mycelium which contained 99.36 U/mg.In single factor test, the suitable medium composition was confirmed. The optimum carbon source was starch that the SOD activity was 206.54 U/mg.The optimum nitrogen source was caseins that the SOD activity was 228.47 U/mg.
To study the antioxidant activity of Glucidum. The ethanolic crude extract showed the most potent capacity for scavenging hydroxyl radical with EC50 values reaching 0.26 mg/ml.When the content of extract was lmg/ml,the scavenging rates on hydroxyl radical was 90.06%.The scavenging hydroxyl radical seems to be positively correlated with the content of total phenols. The content of total flaconoids was 11.82 mg/g and the reducing ability of the crude extract was correlated with the content of total flaconoids.
对栽培灵芝进行不同光质处理,在灵芝不同生长发育时期观测其形态变化并测定抗氧化酶体系中主要酶活性。蓝色光质处理在现蕾期、开伞期、弹孢后期多糖含量均高于对照,且差异显著。红色光质处理孢子粉产量最高。蓝色光质处理POD、CAT活性均高于对照。蓝色光质处理提高了抗氧化酶的活性,对灵芝生长有利,表现为保护效应；同时蓝色光质处理可溶性蛋白含量在生长各时期均高于对照,在灵芝菌盖形成期可溶性蛋白含量最大,在开伞期可溶性蛋白质条带上Rf0.18和0.25处有其特征谱带。
研究灵芝液体培养过程中pH值、菌丝体干重、SOD活性、蛋白质含量和灵芝多糖的变化趋势。在灵芝发酵过程中,灵芝菌丝体SOD比活力高于发酵液。菌丝体SOD比活力最大为99.36 U/mg,是发酵液SOD比活力的5.71倍。通过单因素实验确定产SOD适宜的培养基组成,淀粉为碳源时,灵芝发酵液产发SOD活力最高,SOD比活力为206.54 U/mg。以干酪素为氮源时,灵芝发酵液产发SOD活力最高,SOD比活力为228.47 U/mg。研究灵芝发酵液体外抗氧化作用。灵芝乙醇提取物有较好的清除羟基自由基能力,当提取物浓度达1 mg/ml时,清除率达90.06%,EC5o值为0.26 mg/ml。研究表明总酚含量与灵芝乙醇提取物清除羟基自由基能力密切相关。灵芝乙醇提取物总黄酮含量为11.82 mg/g,与还原能力呈正相关。
In order to provide theoretical basis for cultivation,the effects of different light qualities on the form and anti-oxidative enzyme activities of Ganoderma lucidum ever carried by the spacecraft of China were studied. G.lncidum was planted under different light qualities. Their forms were observed and the activity of antioxidant enzyme system was studied. The content of polysaccharides was higher than CK under blue light in the period of budding, parachute phase, after spore ejection. And the highest yield of spores were got under red light. The blue light induced a increase on the content of MDA,the activities of POD,CAT in the end of growth. Antioxidant enzymes are enhanced by blue light,which presents protective efficacy on G.lucidum. The content of protein under the blue light was higher than CK in the period of growth. There were two different spots under blue light in the electrophoretograms of Glucidum.
With Glucidum as materials, the change tendencies of pH,the dry weight of mycelium,SOD,protein and polysaccharides content were investigated during shake flath cultivation of G.lucidum.The results showed that the G.lucidum had a high SOD activity in mycelium which contained 99.36 U/mg.In single factor test, the suitable medium composition was confirmed. The optimum carbon source was starch that the SOD activity was 206.54 U/mg.The optimum nitrogen source was caseins that the SOD activity was 228.47 U/mg.
To study the antioxidant activity of Glucidum. The ethanolic crude extract showed the most potent capacity for scavenging hydroxyl radical with EC50 values reaching 0.26 mg/ml.When the content of extract was lmg/ml,the scavenging rates on hydroxyl radical was 90.06%.The scavenging hydroxyl radical seems to be positively correlated with the content of total phenols. The content of total flaconoids was 11.82 mg/g and the reducing ability of the crude extract was correlated with the content of total flaconoids.
引文
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