黑龙江省稻瘟病菌致病基因的检测及分布情况研究
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摘要
采用分子标记技术对黑龙江省17县(市)的167个稻瘟病菌携带MPG1, MPS1 MagB, CPKA, MNH1 MgYCA1 MgS11和MgATG5等致病相关基因进行了检测,阐明了8个致病相关基因在黑龙江省稻瘟病菌群体中分布情况。
检测结果表明,不同的致病相关基因在黑龙江省稻瘟病菌群体中的出现频率有明显差异,致病基因MPS1出现频率最高(40.12%)并分布最广,致病基因MPG1其次(出现频率37.13%),致病基因CPKA. MagB、MgATG5、MgYCA1和MgS11的出现频率依次为33.53%、31.14%、29.34%、25.75%和23.35%,没有检测到致病基因MNH1。
应用Wolfe和Schwarzbach(1975)提出的方法将携带相同致病基因的菌株归为一类,依据7个致病基因在理论上可划分为127个致病遗传型。本试验共检测出64个致病遗传型,其中携带5个致病基因的遗传型3个,出现频率为2.4%；携带4个致病基因的遗传型12个,出现频率为10.8%；携带3个致病基因的遗传型21个,出现频率为18.6%；携带2个致病基因的遗传型20个,出现频率为35.3%；携带1个致病基因的遗传型7个,出现频率为25.7%；不携带致病基因的遗传型1个,出现频率为7.2%。说明黑龙江省稻瘟病菌群体的结构比较复杂的,所携带的致病基因数量具有明显差异。
各致病遗传型在黑龙江省出现频率明显不同。致病遗传型127、64、58、59、96、119、91和123号等出现频率较高,其次是致病遗传型61、62、81、93、122和126号。这些致病遗传型在黑龙江省分布较广,但地区间有较大差异。在这些致病遗传型中,绝大多数所携带的致病基因数量比较少,其致病遗传基础比较简单。而携带3个以上致病基因的致病遗传型在黑龙江省出现频率较低,均在局部地区出现,地区间存在着很大差异。表明黑龙江省稻瘟病菌群体结构比较复杂。
各致病菌株遗传型的实际出现频率与理论出现频率相差较大,这可能与致病基因之间的相互作用有关。黑龙江省各稻作区之间稻瘟病菌群体结构存在较大差异,这可能与本地区水稻品种种植结构有关。
The experiment utilized Molecular markers to identify and analysis Pathogenicity genes of Magnaporthe grisea in Heilongjiang Province,which were MPG1、MPS1、MagB、CPKA、MNH1、MgYCA1、MgS11and MgATG5.It clarified the distribution of the eight Pathogenicity genes and pathogenic ability of Magnaporthe grisea group in Heilongjiang Province.
The results showed that the frequency of different Pathogenicity genes was significant different in Heilongjiang Province.The MPS1 had the most widely distributed with the positive frequency up to 52.94%; the second biggest distribution was the Pathogenicity gene MPG1 of which positive frequency was 37.13%; The positive frequencies for CPKA,MagB,MgATG5,MgYCA1 andMgS 11 were 33.74%,31.33%,29.48%, 25.75%and23.51%, respectively;The MNH1 was not detected here.
Different the strains tested carried a significant difference in the number of Pathogenicity genes. Application Wolfe and Schwarzbach(1975) proposed methods will carry the same strain of pathogenic genes classified as a class, based on seven Pathogenicity genes in theory can be divided into 127 types of Pathogenicity genetic.The test detected a total of 64 pathogenic genotype type, five Pathogenicity genes carre 3 genotype type,The frequency was2.4%; four Pathogenicity genes carre 12 genotype type, The frequency was 10.8%; three Pathogenicity genes carre 21 genotype type, The frequency was 18.6%; two Pathogenicity genes carre 20 genotype type,The frequency was35.3%; one Pathogenicity genes carre 7 genotype type, The frequency was 25.7%; zero Pathogenicity genes carre 1 genotype type, The frequency was 7.2%; showing the structure of Magnaporthe grisea is more complex in Heilongjiang Province,but the genes that less Pathogenicity genes carrying mainly, most of the genetic basis of pathogenicity of individual is relatively simple and pathogenicity is not strong, so planting resistant varieties use control.
The pathogenic genotype significantly different frequencies in Heilongjiang Province. The pathogenic genotype of higher frequency is 127、64、58、59、96、119、91and123, the second is 61、62、81、93、122 and 126. These pathogenic genotype widely distributed, but differed greatly among regions in Heilongjiang Province. In these type of genetic pathogenic,most of the genes carried by the number of relatively small,Its relatively simple genetic basis of genetic pathogenic.While carrying three Pathogenicity genes more than genotype is the lower frequencies in Heilongjiang Province, are in the local region, there is great difference between regions. Description structure of Magnaporthe grisea is more complex in Heilongjiang Province.
The actual pathogenic genotype frequency and theoretical frequency difference larger, this may be related to the interaction between the Pathogenicity genes.
The rice blast fungus range Magnaporthe grisea structure are quite different in Heilongjiang Province, the rice area has been formed with the characteristics of this region the structure of Magnaporthe grisea. This may be related to Rice Varietiesin Planting this region related to the structure.
检测结果表明,不同的致病相关基因在黑龙江省稻瘟病菌群体中的出现频率有明显差异,致病基因MPS1出现频率最高(40.12%)并分布最广,致病基因MPG1其次(出现频率37.13%),致病基因CPKA. MagB、MgATG5、MgYCA1和MgS11的出现频率依次为33.53%、31.14%、29.34%、25.75%和23.35%,没有检测到致病基因MNH1。
应用Wolfe和Schwarzbach(1975)提出的方法将携带相同致病基因的菌株归为一类,依据7个致病基因在理论上可划分为127个致病遗传型。本试验共检测出64个致病遗传型,其中携带5个致病基因的遗传型3个,出现频率为2.4%；携带4个致病基因的遗传型12个,出现频率为10.8%；携带3个致病基因的遗传型21个,出现频率为18.6%；携带2个致病基因的遗传型20个,出现频率为35.3%；携带1个致病基因的遗传型7个,出现频率为25.7%；不携带致病基因的遗传型1个,出现频率为7.2%。说明黑龙江省稻瘟病菌群体的结构比较复杂的,所携带的致病基因数量具有明显差异。
各致病遗传型在黑龙江省出现频率明显不同。致病遗传型127、64、58、59、96、119、91和123号等出现频率较高,其次是致病遗传型61、62、81、93、122和126号。这些致病遗传型在黑龙江省分布较广,但地区间有较大差异。在这些致病遗传型中,绝大多数所携带的致病基因数量比较少,其致病遗传基础比较简单。而携带3个以上致病基因的致病遗传型在黑龙江省出现频率较低,均在局部地区出现,地区间存在着很大差异。表明黑龙江省稻瘟病菌群体结构比较复杂。
各致病菌株遗传型的实际出现频率与理论出现频率相差较大,这可能与致病基因之间的相互作用有关。黑龙江省各稻作区之间稻瘟病菌群体结构存在较大差异,这可能与本地区水稻品种种植结构有关。
The experiment utilized Molecular markers to identify and analysis Pathogenicity genes of Magnaporthe grisea in Heilongjiang Province,which were MPG1、MPS1、MagB、CPKA、MNH1、MgYCA1、MgS11and MgATG5.It clarified the distribution of the eight Pathogenicity genes and pathogenic ability of Magnaporthe grisea group in Heilongjiang Province.
The results showed that the frequency of different Pathogenicity genes was significant different in Heilongjiang Province.The MPS1 had the most widely distributed with the positive frequency up to 52.94%; the second biggest distribution was the Pathogenicity gene MPG1 of which positive frequency was 37.13%; The positive frequencies for CPKA,MagB,MgATG5,MgYCA1 andMgS 11 were 33.74%,31.33%,29.48%, 25.75%and23.51%, respectively;The MNH1 was not detected here.
Different the strains tested carried a significant difference in the number of Pathogenicity genes. Application Wolfe and Schwarzbach(1975) proposed methods will carry the same strain of pathogenic genes classified as a class, based on seven Pathogenicity genes in theory can be divided into 127 types of Pathogenicity genetic.The test detected a total of 64 pathogenic genotype type, five Pathogenicity genes carre 3 genotype type,The frequency was2.4%; four Pathogenicity genes carre 12 genotype type, The frequency was 10.8%; three Pathogenicity genes carre 21 genotype type, The frequency was 18.6%; two Pathogenicity genes carre 20 genotype type,The frequency was35.3%; one Pathogenicity genes carre 7 genotype type, The frequency was 25.7%; zero Pathogenicity genes carre 1 genotype type, The frequency was 7.2%; showing the structure of Magnaporthe grisea is more complex in Heilongjiang Province,but the genes that less Pathogenicity genes carrying mainly, most of the genetic basis of pathogenicity of individual is relatively simple and pathogenicity is not strong, so planting resistant varieties use control.
The pathogenic genotype significantly different frequencies in Heilongjiang Province. The pathogenic genotype of higher frequency is 127、64、58、59、96、119、91and123, the second is 61、62、81、93、122 and 126. These pathogenic genotype widely distributed, but differed greatly among regions in Heilongjiang Province. In these type of genetic pathogenic,most of the genes carried by the number of relatively small,Its relatively simple genetic basis of genetic pathogenic.While carrying three Pathogenicity genes more than genotype is the lower frequencies in Heilongjiang Province, are in the local region, there is great difference between regions. Description structure of Magnaporthe grisea is more complex in Heilongjiang Province.
The actual pathogenic genotype frequency and theoretical frequency difference larger, this may be related to the interaction between the Pathogenicity genes.
The rice blast fungus range Magnaporthe grisea structure are quite different in Heilongjiang Province, the rice area has been formed with the characteristics of this region the structure of Magnaporthe grisea. This may be related to Rice Varietiesin Planting this region related to the structure.
引文
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