我国2～7岁儿童维生素B_（12）营养流行病学调查
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摘要
【目的】随机现况调查获取我国2～7岁儿童维生素B12(VB12)缺乏的患病率,研究血清VB12水平与贫血关系,对影响儿童血清VB12水平的相关因素进行分析,探讨诊断VB12缺乏的特异敏感的指标;观察干预性研究探索口服补充VB12的效果。
【方法】1、血清VB12水平的现况调查,了解2～7岁儿童VB12的营养状况;2、观察血清VB12正常儿童与低水平儿童血清半胱氨酸(total homocysteine,tHcy)水平的变化情况;3、口服补充VB12观察血清VB12及tHcy恢复情况;4、测试VB12缺乏儿童口服补充VB12前后智力水平改变情况。
【结果】1、2～7岁儿童血清VB12水平高于成人,2～5岁组儿童血清VB12水平有随年龄增长而降低的趋势,6～7岁组儿童血清VB12水平较稳定;2、我国2～7岁儿童存在VB12缺乏,以边缘性缺乏为主,干预治疗一月后,血清VB12水平恢复正常水平;3、2～7岁儿童血清VB12水平与Hb水平相关,早期VB12缺乏可表现正色素或小细胞性贫血;4、血清VB12缺乏儿童血清tHcy水平明显高于血清VB12正常儿童,干预治疗后血清tHcy水平下降,但敏感性不及血清VB12;5、9例IQ值在55～79的儿童IQ值与血清VB12水平低度相关,差异无统计学意义;且VB12缺乏儿童和正常儿童神经心理行为发育水平相近。干预治疗一月后2～3岁儿童智力水平并无改善,4～7岁儿童智力水平较干预前提高,但差异无统计学意义;6. 2～7岁儿童膳食VB12摄入量的高低决定血清VB12水平的高低。
【结论】1、获得我国2～7岁儿童VB12缺乏的患病率;2、儿童膳食决定VB12营养状况;3、口服VB12是治疗VB12缺乏的安全有效方法;4、测定血清VB12水平是判断VB12缺乏的指标,血清tHcy水平检测可作为VB12缺乏辅助诊断指标;5、部分VB12缺乏儿童出现神经心理行为发育障碍,但VB12水平智力水平低度相关。VB12补充治疗在一定程度可改善B12缺乏儿童智力水平。
【目的】了解我国2～7岁儿童维生素B12(VB12)的营养状态,研究血清VB12水平与贫血的关系,并对影响儿童血清VB12水平的相关因素进行分析;检测血清VB12与VB12代谢物半胱氨酸(total homocysteine,tHcy),比较诊断VB12缺乏指标的特异敏感性;研究VB12减少(VB12缺乏和边缘性缺乏)儿童神经心理行为发育情况。
【方法】2007.5~2008.10,随机抽取北京、珠海、重庆、武汉4城市2~7岁儿童2116例进行体格检查、血常规筛查、膳食调查、血清VB12水平及tHcy水平测定,除去资料不全(溶血、血量不足、问卷填写不完整)114例,有效资料2002例。血清VB12测定采用化学发光免疫法;高效液相色谱法检测血清tHcy的水平。采用Gesell发育量表或Wechsler学前及初小儿童智能量表(WPPSI)对VB12缺乏儿童进行神经心理行为评价。
【结果】1.我国4城市2～7岁儿童平均血清VB12水平为775pg/ml,市区儿童平均血清VB12水平(835pg/ml)高于郊区(720 pg/ml()t=8.381,P=0.000),其中北京840pg/ml、珠海772pg/ml、重庆756pg/ml、武汉735pg/ml,差异有统计学意义(F=8.381,P=0.000)。2. 2～7岁儿童平均血清VB12水平在各年龄组间有差异(F=13.258,P=0.000),其中2～5岁组儿童血清VB12水平有随年龄增长而降低的趋势(r=-0.146,P=0.000)。儿童平均血清VB12水平无性别差异(t=-0.993,P=0.321)。3.4城市2～7岁儿童血清VB12缺乏(血清VB12<200pg/ml)检出率为1.5%,边缘性缺乏(血清VB12 200pg/ml～300pg/ml)检出率为3.2%,市区儿童血清VB12减少(VB12缺乏和VB12边缘性缺乏)检出率(3.6%)低于远郊(5.9%)(χ2=5.560,P=0.018)。其中北京、珠海、重庆、武汉4城市2～7岁儿童血清VB12不足和边缘性缺乏检出率分别为0.4%、3.5%,0.2%、3.0%,0.2%、1.9%,5.2%、4.6%。4. 4城市2～7岁血清VB12水平与Hb水平呈极低度相关(r=0.098,P=0.000)。调查儿童平均Hb水平为125.1 g/L,北京、珠海、重庆、武汉2～7岁儿童平均Hb水平分别为129.2 g/L(市区130.4 g/L高于远郊128.1 g/L()t=3.321,P=0.001)、127.4 g/L(市区129.5 g/L高于远郊125.2 g/L)(t=-3.726,P=0.000)、123.5 g/L(市区125.4 g/L高于远郊121.7 g/L)(t=3.316,P=0.001)、120.3 g/L(市区121.3 g/L高于远郊119.3g/L)(t=-2.399,P=0.017)。北京、珠海、重庆、武汉儿童贫血检出率分别为5.3%(11/513)。5.4%(26/503)、12.6%(60/485)、20.2%(101/500)。5. 2～7岁儿童血清VB12水平与tHcy水平不完全平行,但血清VB12水平与tHcy呈负相关(r=-0.445,P=0.000),正常对照组儿童血清tHcy水平明显低于VB12缺乏组和边缘性缺乏组(F=51.391,P=0.000;)。VB12缺乏组和边缘性缺乏组儿童血清tHcy水平相近(t=-1.044,P=0.300)。6.智力测试结果显示除IQ值<79～55的9例儿童VB12水平在161～292pg/ml,余57例儿童智力测试结果均在正常范围。7.多因素逐步回归分析显示血清VB12水平主要受膳食VB12摄入量的影响(标准偏回归系数=0.116,P=0.000)。4城市市区2～7岁儿童平均膳食VB12摄入量(2.4ug/d)高于郊区(2.0 ug/d)(P<0.05),北京、珠海、重庆、武汉4城市2～7岁儿童平均膳食VB12摄入量分别为2.8μg/d、2.6μg/d、1.9μg/d、1.6μg/d,差异有统计学意义(P<0.001)。
【结论】1. 2～7岁儿童血清VB12水平高于成人;2.我国2～7岁儿童存在VB12缺乏,以边缘性缺乏为主;3. 2～7岁儿童血清VB12水平与Hb水平低度相关,早期VB12缺乏可表现正色素或小细胞性贫血;4.血清VB12缺乏儿童血清tHcy水平明显高于VB12正常儿童,提示血清tHcy水平的检测可作为筛查VB12缺乏的指标之一,但不及血清VB12水平测定稳定;5.早期VB12缺乏儿童中仅部分VB12缺乏儿童出现神经心理行为发育落后;6.学龄前儿童膳食VB12摄入量的高低决定血清VB12水平的高低,增加富含VB12的动物性食品是预防儿童VB12缺乏的有效方法。
【目的】研究VB12缺乏儿童口服补充VB12的效果,探讨治疗儿童VB12缺乏的安全有效方法及口服补充VB12后儿童智力改变情况。
【方法】70例血清VB12 <300pg/ml的儿童血清VB12<200pg/ml者21例,血清VB12 200～300pg/ml者49例。口服VB12片剂100μg/d 4周后复查血清VB12,部分儿童复测神经心理发育情况。
【结果】1、所有VB12缺乏儿童血清VB12水平恢复至正常水平;2、口服VB12治疗后,7例2～3岁儿童智力水平与干预前相近;27例4～7岁儿童智力水平较干预前明显改善。
【结论】1、口服补充VB12制剂能改善儿童VB12营养状况,是治疗儿童VB12缺乏的安全有效方法。2、口服VB12干预治疗后,4～7岁儿童智力水平较干预前明显改善,提示VB12缺乏影响儿童神经心理行为发育。
PARTⅠVITAMIN B12 NUTRITIONAL STATUS IN CHILDREN AGED 2～7 IN CHINA
【objective】To explore the levels of serum vitamin B12 (VB12)of preschool children in 4 cities of China;determine the relationship between the levels of serum VB12 and anemia and analyze the effects of several factors related to the level of serum VB12.;To explore the sensitive and specific markers of VB12 deficiency;investigate the level of intellectual functioning in the children with VB12 deficiency;
【Methods】From May 2007 to October 2008,the weight,height and complete blood count(CBC)were studied in 2116 children aged 2 to 7 years from the four cities(Bejing、Zhuhai、Chongqing、wuhan) in China.The dietary survey、the concentrations of serum VB12 of 2002 of the children were evaluated. The concentrations of serum VB12 were assayed by Chemiluminoimmuno Assay(CLIA). Plasma total homocysteine was determined by using HPLC with fluorescence detection.Psychological tests were performed in the children with VB12 deficiency by Gesell Developmental Schedules(GDS)or Wechsler Preschool and Primary Scale of Intelligence(WPPSI).
【Results】1、The average level of serum VB12 for the children aged 2～7 in China was 775pg/ml,and the level of serum VB12 for the children aged 2～7 in urbans was higher than that in suburbs(P<0.001). The concentrations of serum VB12 of children in Beijing、Zhuhai、Chongqing、Wuhan were 840pg/ml、772 pg/ml、756 pg/ml and 735pg/ml,respectively. 2、The levels of serum VB12 changed with ages and there were no differences between the concentrations of both boys and girls .3、1.5% of the preschool children who were assessed for VB12 levels<200pg/ml were considered the vitamin B12 deficiency,3.2% were considered the vitamin B12 marginal deficiency.The prevalence of low VB12 ( deficiency and marginal deficiency)of children in urbans was lower than that in suburbs.The prevalence of low VB12 of children in Beijing、Zhuhai、Chongqing、wuhan were 0.4% and 3.5%、0.2% and 3.0%、0.2% and 1.9%、5.2% and 4.6%,respectively. There were differences of the prevalence of lowVB12 among the cities. 4、There were low correlations between the levels of serum VBl2 and hemoglobin(P<0.001).The average level of hemoglobin for the children aged 2～7 in China was 125.1 g/L,the average levels of hemoglobin in Beijing zhuhai、chongqing、wuhan were 129.2 g/L(the level of hemoglobin130.4 g/L in urban is higher than that in suburb 128.1g/L)、127.4 g/L(the level of hemoglobin129.5g/L in urban is higher than that in suburb125.2 g/L)、123.5 g/L(the level of hemoglobin125.4g/L in urban is higher than that in suburb121.7 g/L)、120.3 g/L(the level of hemoglobin121.3g/L in urban is higher than that in suburb119.3 g/L)。5、Negative correlations were found between VB12 and tHcy(r=-0.445,P=0.000). The serum tHcy concentration in children with adequate VB12 concentration was lower than that in children with VB12 deficiency(F=51.391,P=0.000). 6、The mean scores of DQ were 104、94、90、96 for 9 children with low VB12;The mean scores of VIQ、PIQ、FIQ were 98、94、103 for 57 children with low VB12 .The data analysized by Wilcoxon test showed that there were no significant differences between the children with lowVB12(deficiency and marginal deficiency)and adequate serum VB12 in intelligent tests.7、The results of multivariate stepwise influenced by the contents of VB12 in the animal foods(bˊ=0.116,P=0.000).The contents of VB12 in animal foods children aged 2～7in urbans were higher than that in suburbs(P<0.05).And the contents of VB12 in animal foods for children in the 4 cities were different(P<0.001).
【conclusions】1、Low serum VB12 of children aged 2～7 was prevalent in China.2、The levels of serum VB12 of children aged 2～7 were higher than that of adults.3、The levels of serum VB12 were lowly correlated with the concentrations of hemoglobin.Macrocytic anemia may not occur in children with VB12 deficiency and small or normal cell anemia can be investigated in the early stage of VB12 deficiency.4、The concentrationof serum tHcy exhibited negative correlations with the levels of serumVB12,respectively and may be regarded as a basic marker of VB12 in the serum.5、 The children with VB12 deficiency may not occur cognitive impairment.6、The nutritional condition of vitamin B12 was different by the diets of the children,it is beneficial for children to consume foods with VB12 to keep the normal level of serum VB12.
PARTⅡORAL VB12 THERAPIES IN CHILDREN WITH VB12 DEFICIENCY
【objective】To investigate the effect of therapy with oral vitamin B12(VB12)on the children with VB12 deficiency,explore the effective and safe way to treat and prevent VB12 deficiency in children. To discuss the effect of VB12 supplementation on the cognitive function of children with VB12 deficiency .
【Methods】70 children with VB12 deficiency were received VB12 100μg/d for 4 weeks. The levels of serum VB12 and the intellectual function were redetected in 34 children.
【Results】The levels of serum VB12 in 70 children were increased after supplementation of VB12 .The mean level of serum VB12 in 70 children was increased from 825pg/ml to 596pg/ml after one month of oral supplementation(t=-22.2,P<0.01).
【conclusions】Oral supplementation of VB12 for the children with VB12 deficiency improve the nutritional condition of VB12. The data showed IQ were same in children of 2~3 years old with VB12 deficiency but that were increased in the children aged 4~7.
【方法】1、血清VB12水平的现况调查,了解2～7岁儿童VB12的营养状况;2、观察血清VB12正常儿童与低水平儿童血清半胱氨酸(total homocysteine,tHcy)水平的变化情况;3、口服补充VB12观察血清VB12及tHcy恢复情况;4、测试VB12缺乏儿童口服补充VB12前后智力水平改变情况。
【结果】1、2～7岁儿童血清VB12水平高于成人,2～5岁组儿童血清VB12水平有随年龄增长而降低的趋势,6～7岁组儿童血清VB12水平较稳定;2、我国2～7岁儿童存在VB12缺乏,以边缘性缺乏为主,干预治疗一月后,血清VB12水平恢复正常水平;3、2～7岁儿童血清VB12水平与Hb水平相关,早期VB12缺乏可表现正色素或小细胞性贫血;4、血清VB12缺乏儿童血清tHcy水平明显高于血清VB12正常儿童,干预治疗后血清tHcy水平下降,但敏感性不及血清VB12;5、9例IQ值在55～79的儿童IQ值与血清VB12水平低度相关,差异无统计学意义;且VB12缺乏儿童和正常儿童神经心理行为发育水平相近。干预治疗一月后2～3岁儿童智力水平并无改善,4～7岁儿童智力水平较干预前提高,但差异无统计学意义;6. 2～7岁儿童膳食VB12摄入量的高低决定血清VB12水平的高低。
【结论】1、获得我国2～7岁儿童VB12缺乏的患病率;2、儿童膳食决定VB12营养状况;3、口服VB12是治疗VB12缺乏的安全有效方法;4、测定血清VB12水平是判断VB12缺乏的指标,血清tHcy水平检测可作为VB12缺乏辅助诊断指标;5、部分VB12缺乏儿童出现神经心理行为发育障碍,但VB12水平智力水平低度相关。VB12补充治疗在一定程度可改善B12缺乏儿童智力水平。
【目的】了解我国2～7岁儿童维生素B12(VB12)的营养状态,研究血清VB12水平与贫血的关系,并对影响儿童血清VB12水平的相关因素进行分析;检测血清VB12与VB12代谢物半胱氨酸(total homocysteine,tHcy),比较诊断VB12缺乏指标的特异敏感性;研究VB12减少(VB12缺乏和边缘性缺乏)儿童神经心理行为发育情况。
【方法】2007.5~2008.10,随机抽取北京、珠海、重庆、武汉4城市2~7岁儿童2116例进行体格检查、血常规筛查、膳食调查、血清VB12水平及tHcy水平测定,除去资料不全(溶血、血量不足、问卷填写不完整)114例,有效资料2002例。血清VB12测定采用化学发光免疫法;高效液相色谱法检测血清tHcy的水平。采用Gesell发育量表或Wechsler学前及初小儿童智能量表(WPPSI)对VB12缺乏儿童进行神经心理行为评价。
【结果】1.我国4城市2～7岁儿童平均血清VB12水平为775pg/ml,市区儿童平均血清VB12水平(835pg/ml)高于郊区(720 pg/ml()t=8.381,P=0.000),其中北京840pg/ml、珠海772pg/ml、重庆756pg/ml、武汉735pg/ml,差异有统计学意义(F=8.381,P=0.000)。2. 2～7岁儿童平均血清VB12水平在各年龄组间有差异(F=13.258,P=0.000),其中2～5岁组儿童血清VB12水平有随年龄增长而降低的趋势(r=-0.146,P=0.000)。儿童平均血清VB12水平无性别差异(t=-0.993,P=0.321)。3.4城市2～7岁儿童血清VB12缺乏(血清VB12<200pg/ml)检出率为1.5%,边缘性缺乏(血清VB12 200pg/ml～300pg/ml)检出率为3.2%,市区儿童血清VB12减少(VB12缺乏和VB12边缘性缺乏)检出率(3.6%)低于远郊(5.9%)(χ2=5.560,P=0.018)。其中北京、珠海、重庆、武汉4城市2～7岁儿童血清VB12不足和边缘性缺乏检出率分别为0.4%、3.5%,0.2%、3.0%,0.2%、1.9%,5.2%、4.6%。4. 4城市2～7岁血清VB12水平与Hb水平呈极低度相关(r=0.098,P=0.000)。调查儿童平均Hb水平为125.1 g/L,北京、珠海、重庆、武汉2～7岁儿童平均Hb水平分别为129.2 g/L(市区130.4 g/L高于远郊128.1 g/L()t=3.321,P=0.001)、127.4 g/L(市区129.5 g/L高于远郊125.2 g/L)(t=-3.726,P=0.000)、123.5 g/L(市区125.4 g/L高于远郊121.7 g/L)(t=3.316,P=0.001)、120.3 g/L(市区121.3 g/L高于远郊119.3g/L)(t=-2.399,P=0.017)。北京、珠海、重庆、武汉儿童贫血检出率分别为5.3%(11/513)。5.4%(26/503)、12.6%(60/485)、20.2%(101/500)。5. 2～7岁儿童血清VB12水平与tHcy水平不完全平行,但血清VB12水平与tHcy呈负相关(r=-0.445,P=0.000),正常对照组儿童血清tHcy水平明显低于VB12缺乏组和边缘性缺乏组(F=51.391,P=0.000;)。VB12缺乏组和边缘性缺乏组儿童血清tHcy水平相近(t=-1.044,P=0.300)。6.智力测试结果显示除IQ值<79～55的9例儿童VB12水平在161～292pg/ml,余57例儿童智力测试结果均在正常范围。7.多因素逐步回归分析显示血清VB12水平主要受膳食VB12摄入量的影响(标准偏回归系数=0.116,P=0.000)。4城市市区2～7岁儿童平均膳食VB12摄入量(2.4ug/d)高于郊区(2.0 ug/d)(P<0.05),北京、珠海、重庆、武汉4城市2～7岁儿童平均膳食VB12摄入量分别为2.8μg/d、2.6μg/d、1.9μg/d、1.6μg/d,差异有统计学意义(P<0.001)。
【结论】1. 2～7岁儿童血清VB12水平高于成人;2.我国2～7岁儿童存在VB12缺乏,以边缘性缺乏为主;3. 2～7岁儿童血清VB12水平与Hb水平低度相关,早期VB12缺乏可表现正色素或小细胞性贫血;4.血清VB12缺乏儿童血清tHcy水平明显高于VB12正常儿童,提示血清tHcy水平的检测可作为筛查VB12缺乏的指标之一,但不及血清VB12水平测定稳定;5.早期VB12缺乏儿童中仅部分VB12缺乏儿童出现神经心理行为发育落后;6.学龄前儿童膳食VB12摄入量的高低决定血清VB12水平的高低,增加富含VB12的动物性食品是预防儿童VB12缺乏的有效方法。
【目的】研究VB12缺乏儿童口服补充VB12的效果,探讨治疗儿童VB12缺乏的安全有效方法及口服补充VB12后儿童智力改变情况。
【方法】70例血清VB12 <300pg/ml的儿童血清VB12<200pg/ml者21例,血清VB12 200～300pg/ml者49例。口服VB12片剂100μg/d 4周后复查血清VB12,部分儿童复测神经心理发育情况。
【结果】1、所有VB12缺乏儿童血清VB12水平恢复至正常水平;2、口服VB12治疗后,7例2～3岁儿童智力水平与干预前相近;27例4～7岁儿童智力水平较干预前明显改善。
【结论】1、口服补充VB12制剂能改善儿童VB12营养状况,是治疗儿童VB12缺乏的安全有效方法。2、口服VB12干预治疗后,4～7岁儿童智力水平较干预前明显改善,提示VB12缺乏影响儿童神经心理行为发育。
PARTⅠVITAMIN B12 NUTRITIONAL STATUS IN CHILDREN AGED 2～7 IN CHINA
【objective】To explore the levels of serum vitamin B12 (VB12)of preschool children in 4 cities of China;determine the relationship between the levels of serum VB12 and anemia and analyze the effects of several factors related to the level of serum VB12.;To explore the sensitive and specific markers of VB12 deficiency;investigate the level of intellectual functioning in the children with VB12 deficiency;
【Methods】From May 2007 to October 2008,the weight,height and complete blood count(CBC)were studied in 2116 children aged 2 to 7 years from the four cities(Bejing、Zhuhai、Chongqing、wuhan) in China.The dietary survey、the concentrations of serum VB12 of 2002 of the children were evaluated. The concentrations of serum VB12 were assayed by Chemiluminoimmuno Assay(CLIA). Plasma total homocysteine was determined by using HPLC with fluorescence detection.Psychological tests were performed in the children with VB12 deficiency by Gesell Developmental Schedules(GDS)or Wechsler Preschool and Primary Scale of Intelligence(WPPSI).
【Results】1、The average level of serum VB12 for the children aged 2～7 in China was 775pg/ml,and the level of serum VB12 for the children aged 2～7 in urbans was higher than that in suburbs(P<0.001). The concentrations of serum VB12 of children in Beijing、Zhuhai、Chongqing、Wuhan were 840pg/ml、772 pg/ml、756 pg/ml and 735pg/ml,respectively. 2、The levels of serum VB12 changed with ages and there were no differences between the concentrations of both boys and girls .3、1.5% of the preschool children who were assessed for VB12 levels<200pg/ml were considered the vitamin B12 deficiency,3.2% were considered the vitamin B12 marginal deficiency.The prevalence of low VB12 ( deficiency and marginal deficiency)of children in urbans was lower than that in suburbs.The prevalence of low VB12 of children in Beijing、Zhuhai、Chongqing、wuhan were 0.4% and 3.5%、0.2% and 3.0%、0.2% and 1.9%、5.2% and 4.6%,respectively. There were differences of the prevalence of lowVB12 among the cities. 4、There were low correlations between the levels of serum VBl2 and hemoglobin(P<0.001).The average level of hemoglobin for the children aged 2～7 in China was 125.1 g/L,the average levels of hemoglobin in Beijing zhuhai、chongqing、wuhan were 129.2 g/L(the level of hemoglobin130.4 g/L in urban is higher than that in suburb 128.1g/L)、127.4 g/L(the level of hemoglobin129.5g/L in urban is higher than that in suburb125.2 g/L)、123.5 g/L(the level of hemoglobin125.4g/L in urban is higher than that in suburb121.7 g/L)、120.3 g/L(the level of hemoglobin121.3g/L in urban is higher than that in suburb119.3 g/L)。5、Negative correlations were found between VB12 and tHcy(r=-0.445,P=0.000). The serum tHcy concentration in children with adequate VB12 concentration was lower than that in children with VB12 deficiency(F=51.391,P=0.000). 6、The mean scores of DQ were 104、94、90、96 for 9 children with low VB12;The mean scores of VIQ、PIQ、FIQ were 98、94、103 for 57 children with low VB12 .The data analysized by Wilcoxon test showed that there were no significant differences between the children with lowVB12(deficiency and marginal deficiency)and adequate serum VB12 in intelligent tests.7、The results of multivariate stepwise influenced by the contents of VB12 in the animal foods(bˊ=0.116,P=0.000).The contents of VB12 in animal foods children aged 2～7in urbans were higher than that in suburbs(P<0.05).And the contents of VB12 in animal foods for children in the 4 cities were different(P<0.001).
【conclusions】1、Low serum VB12 of children aged 2～7 was prevalent in China.2、The levels of serum VB12 of children aged 2～7 were higher than that of adults.3、The levels of serum VB12 were lowly correlated with the concentrations of hemoglobin.Macrocytic anemia may not occur in children with VB12 deficiency and small or normal cell anemia can be investigated in the early stage of VB12 deficiency.4、The concentrationof serum tHcy exhibited negative correlations with the levels of serumVB12,respectively and may be regarded as a basic marker of VB12 in the serum.5、 The children with VB12 deficiency may not occur cognitive impairment.6、The nutritional condition of vitamin B12 was different by the diets of the children,it is beneficial for children to consume foods with VB12 to keep the normal level of serum VB12.
PARTⅡORAL VB12 THERAPIES IN CHILDREN WITH VB12 DEFICIENCY
【objective】To investigate the effect of therapy with oral vitamin B12(VB12)on the children with VB12 deficiency,explore the effective and safe way to treat and prevent VB12 deficiency in children. To discuss the effect of VB12 supplementation on the cognitive function of children with VB12 deficiency .
【Methods】70 children with VB12 deficiency were received VB12 100μg/d for 4 weeks. The levels of serum VB12 and the intellectual function were redetected in 34 children.
【Results】The levels of serum VB12 in 70 children were increased after supplementation of VB12 .The mean level of serum VB12 in 70 children was increased from 825pg/ml to 596pg/ml after one month of oral supplementation(t=-22.2,P<0.01).
【conclusions】Oral supplementation of VB12 for the children with VB12 deficiency improve the nutritional condition of VB12. The data showed IQ were same in children of 2~3 years old with VB12 deficiency but that were increased in the children aged 4~7.
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