秦巴山区野生百合组培特性及其耐热性研究
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摘要
野生种质资源是重要性状遗传研究和新品种培育的基础材料,开展对野生种质资源的繁殖、保存和评价研究对于科学合理地利用野生种质资源具有十分重要的意义。本研究以野百合鳞片为材料,对秦巴山区野生百合组织培养的方法进行了探索,同时对它们的组培特性和耐热性进行了研究,以期为野生百合种质资源的繁殖、保存、利用提供依据。
1.野百合的组织培养结果表明:适宜野百合鳞片灭菌的条件为0.1%Hgcl2处理9min,适宜鳞片不定芽诱导的培养基为MS+6BA2.0mg/L+NAA0.2mg/L,适宜不定芽增殖的培养基为MS+6-BA1.0mg/L+NAA0.2mg/L,适宜组培苗生根结鳞茎的蔗糖用量为90g/L、激素用量为6-BA0.1mg/L+NAA0.1mg/L、多效唑用量为10mg/L,适宜组培苗移栽的炼苗时间是7天。
2.秦巴山区6种野生百合组培特性比较结果表明:鳞片0.1%升汞灭菌9min,岷江百合污染率最高,其次为山丹、野百合、宜昌百合、兰州百合,卷丹最低。在培养基MS+6BA2.0mg/L+NAA0.2mg/L上的诱导率野百合最高,其次为卷丹、兰州百合、岷江百合、山丹,宜昌百合最低。在培养基MS+6BA1.0mg/L+NAA0.2mg/L上的增殖率兰州百合最高,其次为野百合、宜昌百合、卷丹、山丹,岷江百合最低。在1/2MS+NAA0.3mg/L的培养基上,卷丹高度最高,其次为兰州百合、宜昌百合、岷江百合、野百合,山丹高度最低;野百合根最多,其次为宜昌百合、兰州百合、卷丹、山丹,岷江百合根最少;兰州百合根最长,其次为野百合、卷丹、山丹、宜昌百合,岷江百合根最短。
3.秦巴山区6种野生百合耐热性比较:以4个栽培百合Prato、Conca D`or、Siberia、Sorbnne为对照,以组培苗为材料对秦巴山区6种野生百合的耐热性进行了比较研究,结果表明:宜昌百合(L.leucanthum)、Prato、山丹(L.pumilum)的耐热较强,野百合(L.brownii)、卷丹(L.lancifolium)的耐热性中等,岷江百合(L.regale)、Sorbnne、兰州百合(L.davidii)、Conca D`or、Siberia耐热性较弱。
Wild germplasm resources are the basic materials for genetic research and plant breeding. Developing the research on propagation, preservation and evaluation of wild germplasm resources is quit important for using scientifically and reasonably. Lilium brownii Thunb bud scales are used as materiasl and the method of tissue culture is explored for wild lilies from Qin-ba Mountain. Meanwhile, the research of their tissue culture properties and heat resistance were studied so as to provide proofs of propagation, preservation and utilization for wild lily germplasm resources.
1. The results of tissue culture on L.brownii scales showed that the appropriate sterilizing time of 0.1% Hgcl2 was 9 minutes and the suitable medium on which the adventitious buds could be induced directly from scales was MS+BA 2.0mg/L +NAA0.05mg/L. The optimum proliferation medium for adventitious buds was MS+BA1.0mg/L+NAA0.2mg/L. The appropriate condition in which bud scales could be formated and enlarged was 6-BA 0.1mg/L+NAA 0.1mg/L, supplied with 90g/L sucrose and 10mg/L hormones.The best acclimatization time on transplanting was 7 days.
2.Comparison of tissue culture properties among six wild lily species from Qin-ba Mountain indicated that sterilizing time of 0.1% Hgcl2 was 9 minutes, the contamination rate of L.regale was the highest , and that of L.lancifolium was the lowest, the others wild lily species were L.pumilum, L.brownii , L.leucanthum, L.davidii in order.On the medium of MS+6BA2.0mg/L+NAA0.2mg/L, the L.brownii had the highest induction rate, of which L.leucanthum was the lowest, and the others were L.lancifolium, L.davidii var.unicelor, L.regale ,L.pumilum in sequence. On the medium of MS+ 6BA1.0mg/L+ NAA0.2mg/L, the proliferation rate of L.davidii was the highest, and that of L.regale was the lowest, and that of the others in the following order were L.brownii, L.leucanthum, L.lancifolium, L.pumilum.On the medium of 1/2MS+NAA0.3mg/L, the L.lancifolium had the highest plant height, and on the contrary L.pumilum had the lowest height, and the others wild lily species were L.davidii var.unicelor, L.leucanthum, L.regale, L.brownii in oder. With the same medium the root number of L.brownii was most, and that of L.regale was lest, that of the others in the following order were L.lancifolium, L.davidii var.unicelor, L.lancifolium,L.pumilum. The length of wild lily species on the same condition were L.davidii, L.brownii, L.lancifolium, L.pumilum ,L.leucanthum, L.regale in sequence.
3.The heat tolerance of six wild lily species was tested by using plantlets in vitro and four lily cultivars (Prato, Conca D`or, Siberia and Sorbnne) were used as controls. The result demonstrated that L.leucanthum, Prato and L.pumilum were the most tolerant to the heat treatment, tolerance of L.brownii F.E , L.lancifolium were moderate, and L.regale, Sorbnne, L.davidii, Conca D`or, Siberia were sensitive to heat.
1.野百合的组织培养结果表明:适宜野百合鳞片灭菌的条件为0.1%Hgcl2处理9min,适宜鳞片不定芽诱导的培养基为MS+6BA2.0mg/L+NAA0.2mg/L,适宜不定芽增殖的培养基为MS+6-BA1.0mg/L+NAA0.2mg/L,适宜组培苗生根结鳞茎的蔗糖用量为90g/L、激素用量为6-BA0.1mg/L+NAA0.1mg/L、多效唑用量为10mg/L,适宜组培苗移栽的炼苗时间是7天。
2.秦巴山区6种野生百合组培特性比较结果表明:鳞片0.1%升汞灭菌9min,岷江百合污染率最高,其次为山丹、野百合、宜昌百合、兰州百合,卷丹最低。在培养基MS+6BA2.0mg/L+NAA0.2mg/L上的诱导率野百合最高,其次为卷丹、兰州百合、岷江百合、山丹,宜昌百合最低。在培养基MS+6BA1.0mg/L+NAA0.2mg/L上的增殖率兰州百合最高,其次为野百合、宜昌百合、卷丹、山丹,岷江百合最低。在1/2MS+NAA0.3mg/L的培养基上,卷丹高度最高,其次为兰州百合、宜昌百合、岷江百合、野百合,山丹高度最低;野百合根最多,其次为宜昌百合、兰州百合、卷丹、山丹,岷江百合根最少;兰州百合根最长,其次为野百合、卷丹、山丹、宜昌百合,岷江百合根最短。
3.秦巴山区6种野生百合耐热性比较:以4个栽培百合Prato、Conca D`or、Siberia、Sorbnne为对照,以组培苗为材料对秦巴山区6种野生百合的耐热性进行了比较研究,结果表明:宜昌百合(L.leucanthum)、Prato、山丹(L.pumilum)的耐热较强,野百合(L.brownii)、卷丹(L.lancifolium)的耐热性中等,岷江百合(L.regale)、Sorbnne、兰州百合(L.davidii)、Conca D`or、Siberia耐热性较弱。
Wild germplasm resources are the basic materials for genetic research and plant breeding. Developing the research on propagation, preservation and evaluation of wild germplasm resources is quit important for using scientifically and reasonably. Lilium brownii Thunb bud scales are used as materiasl and the method of tissue culture is explored for wild lilies from Qin-ba Mountain. Meanwhile, the research of their tissue culture properties and heat resistance were studied so as to provide proofs of propagation, preservation and utilization for wild lily germplasm resources.
1. The results of tissue culture on L.brownii scales showed that the appropriate sterilizing time of 0.1% Hgcl2 was 9 minutes and the suitable medium on which the adventitious buds could be induced directly from scales was MS+BA 2.0mg/L +NAA0.05mg/L. The optimum proliferation medium for adventitious buds was MS+BA1.0mg/L+NAA0.2mg/L. The appropriate condition in which bud scales could be formated and enlarged was 6-BA 0.1mg/L+NAA 0.1mg/L, supplied with 90g/L sucrose and 10mg/L hormones.The best acclimatization time on transplanting was 7 days.
2.Comparison of tissue culture properties among six wild lily species from Qin-ba Mountain indicated that sterilizing time of 0.1% Hgcl2 was 9 minutes, the contamination rate of L.regale was the highest , and that of L.lancifolium was the lowest, the others wild lily species were L.pumilum, L.brownii , L.leucanthum, L.davidii in order.On the medium of MS+6BA2.0mg/L+NAA0.2mg/L, the L.brownii had the highest induction rate, of which L.leucanthum was the lowest, and the others were L.lancifolium, L.davidii var.unicelor, L.regale ,L.pumilum in sequence. On the medium of MS+ 6BA1.0mg/L+ NAA0.2mg/L, the proliferation rate of L.davidii was the highest, and that of L.regale was the lowest, and that of the others in the following order were L.brownii, L.leucanthum, L.lancifolium, L.pumilum.On the medium of 1/2MS+NAA0.3mg/L, the L.lancifolium had the highest plant height, and on the contrary L.pumilum had the lowest height, and the others wild lily species were L.davidii var.unicelor, L.leucanthum, L.regale, L.brownii in oder. With the same medium the root number of L.brownii was most, and that of L.regale was lest, that of the others in the following order were L.lancifolium, L.davidii var.unicelor, L.lancifolium,L.pumilum. The length of wild lily species on the same condition were L.davidii, L.brownii, L.lancifolium, L.pumilum ,L.leucanthum, L.regale in sequence.
3.The heat tolerance of six wild lily species was tested by using plantlets in vitro and four lily cultivars (Prato, Conca D`or, Siberia and Sorbnne) were used as controls. The result demonstrated that L.leucanthum, Prato and L.pumilum were the most tolerant to the heat treatment, tolerance of L.brownii F.E , L.lancifolium were moderate, and L.regale, Sorbnne, L.davidii, Conca D`or, Siberia were sensitive to heat.
引文
[1]陈俊愉,程绪坷.中国花经[M].上海:上海文化出版社,1989.
[2]鲁涤非.花卉学[M].北京:中国农业出版社,1997.
[3]中国科学院中国植物志编辑委员会.中国植物志,第十四卷.[M]北京:科学出版社1980, 118.
[4] Buxbaum F. Die Entwicklungslinien der Lilioldeae [J]. Bot Archiv Band,1937,38:213-398.
[5] Stoker F C. 1933.The Environment of Lilies in Nature [J]. London: Royal Horticultural Socity Lily Yearbook,11-57.
[6] Lighty R W. Evolutionary Trends in Lilies[J].London:Royal Horticuitural Society, Lily yearbook.1968,40-44.
[7]杨利平,杨青杰.百合研究综述[J].韶关学院学报(自然科学版),2003,24(9):87-91.
[8]张延龙,牛立新.现代百合花产业进展[J].杨凌职业技术学院学报.2002,1(1):12-15.
[9]赵祥云,陈新露,王树栋.秦巴山区的百合花[J].植物杂志.1991,18(5):22-22.
[10]赵祥云,王树栋,陈新露等.中国百合二十年研究进展[C].中国花卉科技二十年.北京:科技出版社,2000.515-525.
[11]陈俊愉.中国花卉品种分类学[M].北京:中国林业出版社, 2001.
[12] Baranova MV. A synopsis of the system of the genus Lilium(Liliaceae) (in Russian) [J]. Bot Zhur,1988, 73:1319-1329.
[13] Smyth DR, Kongsuwan K, Wisudharomn S. A survey of C-band patterns in chromosomes of Lilium (Liliaceae) [J]. Plant Syst Evol,1989,163:53-69.
[14] Asanoj Y .A numerical taxonomic study of the genus Lilium in Japan [J]. J Fac Agr Hokkaido Univ,1986, 62 :333-341.
[15]鲍隆友,周杰,刘玉军.西藏野生百合属植物资源及其开发利用[J].中国林副特产. 2004(2):54-55.
[16]赵祥云,王树栋等.百合[M]北京:中国农业出版社,2000.1
[17]宁云芬,周厚高,黄玉源等:百合种球繁育的研究进展.[J]仲恺农业技术学院学报,2002,15(2):66-69
[18]汪有良,南京百合快速繁殖[J].江苏林业科技,1995,22(1):35-43.
[19]陈心启,许介眉,梁松筠等.中国植物志(第十四卷)[M].北京:科学出版社,1980.118-157.
[20]赵祥云,陈新露,王树栋.等.秦巴山区野生百合资源研究初报[J].西北农业大学学报.1990,18(4):80-84
[21]郎楷永.中国的百合[J].科技导报,2003,(7):24-37.
[22]张延龙,牛立新,梁建丽等.秦岭野生百合生长发育的研究[C].园艺学进展第六辑,2004,622-625.
[23]中国农学会遗传资源学会.中国作物遗传资源[M].北京:中国农业出版社,1994.1163-1164.
[24]彭隆金.百合杂交研究.植物引种驯化集刊[C].第八集.北京:科学出版社,1993.99-100.
[25]史跃林.组培苗的生育环境与调节[J].植物生理学通讯,1990(3).
[26]陈为民,宋为民.卷丹和青岛百合的组织培养及植株分化[J].植物生理学通讯,1982,(2):35-36
[27]黄济明.百合的组织培养和试管内诱发多倍体试验[J].园艺学报,1983, 10(2):125-128
[28]杨柏云,罗丽萍,蔡奇英.切花百合组织培养的研究闭.南昌大学学报(理科版),2000,24(4):323-331
[29]罗丽萍,杨柏云,章敏华等.百合组织培养阴.中草药,2001,32(7):640-64
[30]宋建英,叶建仁.‘马可’百合组织培养研究[J].南京林业大学学报,2006,30(4):73-76
[31]马惠,郭扶兴,周俊彦,等.百合叶片愈伤组织的诱导和植株再生[J].植物生理学通讯,1992,4(28):284-287
[32]卢其能.龙牙百合的组织培养和快速繁殖[J].江西农业学报,2002,14(4):43-46
[33]马纯艳.有斑百合叶片的组织培养[J].沈阳师范学院学报(自然科学版),2002,20(3):219-21
[34]王志,朱万芹,李凤沈.新普百合叶片的组织培养[J].山东农业科学,2005,(5):10-15
[35]洪艳华,殷广峰,张立军.百合脱毒及病毒检测技术研究进展[J].沈阳农业大学学报,2003,34(3):225-227
[36] Mori K,Hosokawa D,Watabe M.Studies on multiplieation and distribution of virushe in plants by use of fluoreseent antibody technique.I. Multiplicaion and distribution of viruses in shoot apices[J]. Ann phytopath Soc Japan,1982,48:433-443
[37] Walkey DGA,Production of virus-free plant.In:Tseng C K ed. Applied plant virology[M].New York:Science Press,1991:270-290
[38]赵祥云,程廉,邢尤美等.百合珠芽组培及脱毒研究[J].园艺学报,1993,20(3):284-288
[39]李浚明.植物组织培养教程[M]北京:中国农业大学出版社,2002,14-17
[40]李耿光,张兰英.庸香百合茎段组织培养快速繁殖[J].云南植物研究,1983,5(3):327-33
[41]陈小兰,胡琼花,王红霞.金百合的离体快速繁殖[J].植物生理学通讯,2000,36(4):334
[42]席梦丽,王节萍,章静娟,等.宜兴百合脱毒技术[J].江苏农业学报,2001,17(l):49-51
[43]王红霞,胡琼花,陈小兰.通江百合的组织培养[J].植物生理学通讯,2O00,36(2):132
[44]郭海滨,雷家军.卷丹百合鳞片及珠芽组织培养[J].中国农学通报,2006,22(2):72-74
[45]诸云霞,张永青,杨红娟等.百合花药愈伤组织诱导中的基因型差异[J].上海农业学报,2002,18(3):17-20
[46] SharpWR,RashinRS,SomerHE.HaploidinLilium[J].Phytomorphology,1971,(21):331-337
[47]谷祝平,郑国錩.从百合花粉诱导花粉植株的研究[J].植物学报,1982,24(1):28-31
[48]刘雅莉,张剑侠,潘学军.东方百合‘索邦’的花器官培养与快速繁殖[J].西北植物学报,2004,24(12):2350-2354
[49]王月芬,奚元龄,陆维忠等.兰州百合体细胞组织培养的培养基成分[J].江苏农业学报,1987,3(2):18-24
[50]黄惠英.东方百合的离体培养[J].甘肃农业大学学报,2000,35):450-453
[51]龙春林,程治英,王俐等.兰州百合器官离体培养外植体位置效应观察[J].云南植物研究,2004,26(2):221-225
[52]王蒂.植物组织培养[M].北京:中国农业出版社,2004:36-38,261-263
[53]张文种.不同激素配比对庸香百合鳞茎芽诱导的影响[J].亚热带植物学,2002,3l(l):2l-24
[54]王林,杨万年.宜昌百合小鳞茎诱导的初步研究[J].氨基酸和生物资源,2005,27(3):7-8
[55]李倩中,刘晓青,陈尚平.不同浓度IBA和6-BA对百合组织培养的影响[J].南京农专学报,2003,19(2):43-45
[56]孙君社,方晓华.植物激素对百合鳞片愈伤组织生长的影响[J].中国农业大学学报,2001,6(2):58-61
[57]潘锦旭,邢震,郑维列.西藏卓马百合组织培养技术研究[J].中国生态农业学报,2002,10(2):26-28
[58]冷肖荀,王青华.外植体和培养因子对百合不定芽诱导的影响[J].河北科技,2000,(l):l-2
[59]张德华,焦成瑾,王亚馥等.小麦体细胞胚胎发生及其与合子胚比较[J].兰州大学学报,30(增刊):55-59
[60]崔凯荣,戴若兰.植物体细胞胚发生的分子生物学[M].北京:科学出版社,2000:21-25
[61]黄学林,李筱菊.高等植物组织培养离体培养的形态建成及其调控[M].北京:科学出版社,1995:18-30
[62]邝哲师,周丽侬,马雪荡,等.香蕉和荔枝体胚发生两种不同途径的研究[J].农业生物技术学报,1998,6(3):21-22
[63]郊艳红,熊庆娥.植物体细胞胚胎发生的研究进展[J].四川农业大学学报,2003,2(l):30(增刊):55-59
[64]王亚馥,徐庆,刘志学.红豆草细胞悬浮培养中体细胞胚的形成[J].实验生物学报,1990,23(3): 369-373
[65]周俊彦.植物体细胞在组织培养中产生的胚状体I.植物体细胞的胚状体发生[J].植物生理学报,1981,7(4):389-396
[66]崔凯荣,任红旭,邢更妹,等.构祀组织培养中抗氧化酶活性与体细胞胚胎发生相关性的研究[J].兰州大学学报(自然科学版),1998,34(3):93-99
[67]周俊彦,郭扶兴.细胞分裂素类物质在植物体细胞胚发育中的作用[J].植物生理学通讯,1996,32(4):247-253
[68]王耀芝,丁惠宾.被子植物胚胎学实验图谱,烟草胚的发育[M].兰州大学出版社,1992:92-94
[69]李景原,李大卫,毛使民.山丹的组织培养和再生鳞茎的形态解剖学研究[J].河南师范大学学报(自然科学版),1995,23(1):102-104
[70]罗凤霞,徐桂花,金丽丽等.新铁炮百合微繁的研究[J].沈阳农业大学学报,2000,31(3):254-257
[71]杨柏云,罗丽萍,蔡奇英.切花百合组织培养的研究[J].南昌大学学报(理科版),2000,24(4):323-32
[72]刘菊华,金志强,徐壁玉.龙牙百合的植株再生与遗传转化[J].分子植物育种,2003,1(4):465-474
[73]杜捷,王刚,幸亭泰等.兰州百合继代培养过程中的染色体变异.西北师范大学学报,2003,39(2): 61-65
[74]王爱勤,周歧伟,何龙飞等.百合试管结鳞茎的研究[J].广西农业大学学报,1998,17(1):7l-75
[75]傅玉兰,何风群.影响百合试管鳞茎增殖因素的研[J].安徽农业大学学报,2001,28(2):179-181
[76]方少忠,蔡宣梅,林真等.CEPA和KT对东方百合仔球复壮的影响[J].江西农业大报,2004,26(6) :893-895
[77]张延龙,梁建丽,牛立新.东方百合试管鳞茎膨大的研究[J].西北农林科技大学学报,2006,34(6): 75-78
[78] Joung Hyang Young and Sung Moonsuk.In vitro Propagation of L.oriental hybrid'Casa Blanca'and L.leichtlinii var.tigrinum as influenced by growth regulators and cultural explant[J].Journal of Agriculture Science.1995,37(1):378-383.
[79] Lim S,Seon J H,Paek K Y,etal.Development of pilotscale process for mass production of Lilium bulblet in vitro[J].Acta Hort.1998,461:237-241.
[80] Nhut D T.Micropropagation of lily(L,longiflorum)via in vitro stem node and pseudo bulblet culture[J].Plant Cell Reports,1998,17:913-916.
[81] Dennis P,Stimart,Peter D.Ascher and Harold F.Wildins.Overcoming dormancy in L.longi-florum bulblets produced in tissue culture[J].Hort.Sci.1982,107(6):1004-1007.
[82] Kato T.Physiological Studies on bulb formation and dormancy in the onion plant.VLThe relation between the metabolism of ribberellin and nucleic acid and bulbing phenomenon[J].Jap Soc Hort Sci,1965,34:305.
[83] Levy D,Kedar N,karaeinque K.Effect of the plon on bulbing of onion under non-inductivephotoperiod[J].Hort Science,1973,8:228.
[84] Levy D.Kedar N.Effect of ethrel on growth and bulb initiation in onion [J].HoctScience, 1970,5:80.
[85]田长恩.多胺在离体培养的植物组织形态建成中的作用[J].植物生理学通讯,1992,28(3):230-232.
[86] Tanimoto S.Matsubara Y.Stimulating effect of spermine on Lmlblet formation in bulb-scale Segments of Lilium longiflorum[J].Plant Cell Reports.1995,15:297-300.
[87] English J.Bonner j,Snit H.The wound hormones of plants.The isolation of a crystalline active substance[J].Proc Natl Acad Sci USA,1939,25:323-329.
[88] Marinangeli P,Devalis R,Deelmastro S.Curvetto N.Stimulation of blllblet differentiation by traumatic acid in Lilium blllb-scale segments cultured in vitro[J].Biocell,1995,19:189-195.
[89] Marinangeli P.Curvtto N Bulb quality and traumatic acid influence bulblet formation from scaling in Lilium species and hybrids[J].Hort Science.1997,32(4):739-741.
[90] Marinangeli P,Delmastro S,Curvtto N.Influence of DB-cAMP adenosine forskolin alld traumatic acid on invitro bulbing of Lilium longiflorum[J].Hort Science.1998,33(1):l5l-152.
[91] Takayama S and Misawa M.Differentiation in Lilium bulb grown in vitro,effect of various cultural conditions[J].Physiologia Plantarum.1979,46(2):184-190.
[92]王爱勤,周歧伟,何龙飞等.百合试管结鳞茎的研究[J].广西农业大学学报,1998,17(l):71-75.
[93] Lim S,Seon J H,Paek K Y,etal.Development of pilotscale process for mass production of Lilium bulblet in vitro[J].Acta Hort,1998,461:237-241.
[94]阮少宁,杨华,梁一池等.香水百合组织培养的试验研究[J].福建林学院学报,2001,21(2):142-145.
[95]江如蓝.多效唑对观赏植物的生物学效应及其在植物组织培养上的应用[J].仲恺农业技术学院学报,2000,13(1):50-57.
[96]唐前瑞,谭艳云,于晖.多效唑对非洲菊试管苗生根的影响[J].湖南农业大学学报,22(1):29-32.
[97]徐启江,李玉花,陈典.PP333对分蘖洋葱试管苗增殖和生根的影响[J].植物生理学通讯,40(3):314-316.
[98]朱道圩,张会丽,王锦等.多效唑对大花高代组培苗生长的效应[J].植物生理学通讯,2006,42(2): 232-234.
[99]徐琼,彭志云.多效唑与蔗糖对不同阶段彩色马蹄莲组培苗质量的影响[J].农业网络信息,2006,6:165-167.
[100]王家福,陈振光.百合快速繁殖条件的优化[J].福建农业大学学报,1999,28(2):152-156.
[101] Park NB.Effect of temperature,scle position and growth regulators on the bulblet formation and growth during scale propagation of Lilium[J].Acta Horticulture,1996,414:257-262.
[102] Jeong JH.In vitro propagation of bulb scale section of several korean native lilies[J].Acta Horticulture,1996,414:269-276.
[103]金淑梅,杨利平,吕品等.细叶百合中内源激素的变化[J].东北林业大学学报,2005,33(1):20-22.
[104] Langens-Gerrits-Metal.Bulb growth in Lily regenerated in vitro [J].Acta Horticulture, 1997, 430:267-273.
[105]曹孜义,李唯,王希圣.环境因素和ABA对葡萄试管苗气孔开闭的影响[J].植物生理报,1993,(19)4: 372.
[106]丰锋.提高试管苗移栽成活率的技术研究[J].中国南方果树,2001,30(4):63-64.
[107]蒲秀琴,薛寒清.百合组培苗移栽前的壮苗技术研究[J].青海农林科技,2007,1:59-60.
[108]韦三立.花卉无土栽培[M].北京:中国林业出版社,2001.
[109]夏文孝,郭达初,金敏信.环境条件及基质对试管苗移栽成活率的影响[J].浙江农业学报,1994,6,(3): 171-175.
[110]王凤兰,周厚高等.麝香百合抗热性生理生化指标及综合评价初探[J].广西植物2005,28(2): 261-264
[111]王凤兰,周厚高等.麝香百合基因型抗热性差异及其早期鉴定[J].湖北农业科学,2007,46(2): 253-255
[112]王凤兰,周厚高,黄玉源.4个新铁炮百合品系幼苗的抗热指标测定[J].仲恺农业技术学院学报,2003,16(2):38-42
[113]王凤兰,周厚高,黄子峰,等.新铁炮百合叶片抗热性形态指标初探[J],湖北农学院学报,2004,24(2):102-105.
[114]周斯建,义鸣放,穆鼎,高温胁迫下铁炮百合幼苗形态及生理反应的初步研究[J].园艺学报.2005,32(1):145～147
[115]张施君,周厚高,潘文华,余卓玲.新铁炮百合的耐热性生理初步研究[M].雷建军主编:园艺学进展(第五辑).广州:广州出版社,2002.665～669
[116]王光耀,刘俊梅,张仪,等.菜豆四个不同抗热性品种的气孔特性[J].农业生物技术学报,1999,7(3):267-270.
[117]潘宝贵,王述彬,刘金兵.高温胁迫对不同辣椒品系苗期光合作用的影响[J].江苏农业学报,2006,22(2):137-140.
[118]彭永宏,章文才.猕猴桃叶片耐热性指标研究[J].武汉植物学研究,1995,13(I):70-74.
[119]叶小梅,卢忠华,蔡丽萍,等.4种引进月季品种叶片耐热性生理生化指标初探[J].广东林业科技,2007,23(1):80-82.
[120]王向阳,王淑俭,彭文博,等.高温对小麦幼苗生理特性影响的初步研究[J].河南农业大学学报,1992,26(1):9-15.
[121]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究.II.叶片水分和蛋白质代谢与耐热性[J].福建农业大学学报,1996,25(4):490-493.
[122]陈火瑛,江隆植,张建华.萝卜抗热性鉴定技术[J].上海蔬菜,1992,(3):15-16.
[123]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社. 1995.199-215.
[124]刘存德.栽培植物的抗性鉴定[J].植物生理学通讯,1957,(3):67-71.
[125]陈立松,刘星辉.高温对桃和抽细胞膜透性和光合色素的影响[J].武汉植物学研究,1997,15(3):233-237.
[126] Viering A,Nguyen H T,Heat-shock gene expression in diploid wheat genotypes differing in thermal tolerance[J]. Crop Sci,1992,32:370-377.
[127]李绍鹏.芒果叶片抗旱性鉴定的初步研究[J].热带作物研究,1993,(4):56-59.
[128]罗少波,李智军,周微波等.大白菜品种耐热性的鉴定方法[J].中国蔬菜,1996,(2):16-18.
[129]杨丽薇,王景义,梁惠芳等.早熟大白菜耐热性鉴定技术[J].北方园艺,1996,(6):5-6.
[130]马德华,庞金安,霍振荣等.黄瓜对不同温度逆境的抗性研究[J].中国农业科学,1999,32(5):28-35.
[131]黄莺,赵致.杂交玉米品种抗早性生理指标及综合评价初探[J].种子,2001,(1):12-14.
[132]肖顺元,赵大中.柑桔叶片耐热的生理生化指标[J].果树科学,1990,(7):217-220.
[133]关义新,戴俊英,陈军等.土壤千早下玉米叶片游离脯氨酸的累积及其与抗早性的关系[J].玉米科学,1996,4(1):43-58.
[134] Tahir M.Signh M.Assessment of screening techniques for heat tolerance in wheat[J]. Crop Sci., 1993,33:740-744.
[135] Bery J Aetal. Respones of macrophytes to temperature. In:Physiological Plant Ecology1. Respones to Physical Environment[J]. Heidelberg: Springer Verlag,1981,277-288.
[136]邹琦.小麦高温伤害与高温适应[J].植物学报,1988,30(4):388-395.
[137]谷崇光,陈文,许启新等.电导百分率法测定黄瓜耐热性[J].上海蔬菜,1991,(I):33-34.
[138]罗少波,周微波,罗剑宁,等.黄瓜品种耐热性强度鉴定方法比较[J].广东农业科学,1997,(6):23-24
[139]陆世钧,乔炳根.早熟大白菜耐热性与EC值关系初探[J].上海蔬菜,1990,(2):35.
[140]司家钢,孙日飞,吴飞燕等.高温胁迫对大白菜耐热性相关生理指标的影响[J].中国疏菜,1995,(4):4-6,15.
[141]曾韶西,王以柔,刘鸿先,低温下黄瓜幼苗子叶硫氢基(SH)含量变化与膜脂过氧化[J].植物学报,1991,33(1):50-54.
[142]李成琼,宋洪元,雷建军等.甘蓝耐热性鉴定研究[J].西南农业大学学报,1998,20(4):198-201.
[143]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究.I.根系生理活动与耐热性[J].福建农业大学学报,1996,25增刊):490-493.
[144]刘维信,曹寿椿.高温对不结球大白菜细胞膜透性过氧化物酶活性等的影响[J].南京农业大学学报,1992,15(3):115-117.
[145]姚元干,石雪晖,杨建国等.辣椒叶片耐热性生理生化指标探讨[J].湖南农业大学学报,1998,24(2):119-122.
[146] Martinean J R,Specht J E.Temperature tolerance in soybeans[J].Crop Science,1979,19:75-81
[147]陈少裕.膜脂过氧化与植物逆境胁迫[J]植物学通报,1989,6(4):211-217.
[148]周人纲,樊志和,李晓芝等.热锻炼对小麦叶片细胞膜及有关酶活性的影响[J].作物学报,1995,(5):568-572.
[149]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究III.酶性和非酶性活性氧清除能力与耐热性[J].福建农业大学学报,1997,26(4):498-501.
[150]吴国胜,曹婉虹,王永健等.细胞膜热稳定性及保护酶与大白菜耐热性的关系[J].园艺学报,1995,22:353-358
[151]李合生,孙群.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999.258-263
[152]周厚高,江如蓝,王凤兰等.百合[M].广州:广东科技出版社,2003.15-17
[2]鲁涤非.花卉学[M].北京:中国农业出版社,1997.
[3]中国科学院中国植物志编辑委员会.中国植物志,第十四卷.[M]北京:科学出版社1980, 118.
[4] Buxbaum F. Die Entwicklungslinien der Lilioldeae [J]. Bot Archiv Band,1937,38:213-398.
[5] Stoker F C. 1933.The Environment of Lilies in Nature [J]. London: Royal Horticultural Socity Lily Yearbook,11-57.
[6] Lighty R W. Evolutionary Trends in Lilies[J].London:Royal Horticuitural Society, Lily yearbook.1968,40-44.
[7]杨利平,杨青杰.百合研究综述[J].韶关学院学报(自然科学版),2003,24(9):87-91.
[8]张延龙,牛立新.现代百合花产业进展[J].杨凌职业技术学院学报.2002,1(1):12-15.
[9]赵祥云,陈新露,王树栋.秦巴山区的百合花[J].植物杂志.1991,18(5):22-22.
[10]赵祥云,王树栋,陈新露等.中国百合二十年研究进展[C].中国花卉科技二十年.北京:科技出版社,2000.515-525.
[11]陈俊愉.中国花卉品种分类学[M].北京:中国林业出版社, 2001.
[12] Baranova MV. A synopsis of the system of the genus Lilium(Liliaceae) (in Russian) [J]. Bot Zhur,1988, 73:1319-1329.
[13] Smyth DR, Kongsuwan K, Wisudharomn S. A survey of C-band patterns in chromosomes of Lilium (Liliaceae) [J]. Plant Syst Evol,1989,163:53-69.
[14] Asanoj Y .A numerical taxonomic study of the genus Lilium in Japan [J]. J Fac Agr Hokkaido Univ,1986, 62 :333-341.
[15]鲍隆友,周杰,刘玉军.西藏野生百合属植物资源及其开发利用[J].中国林副特产. 2004(2):54-55.
[16]赵祥云,王树栋等.百合[M]北京:中国农业出版社,2000.1
[17]宁云芬,周厚高,黄玉源等:百合种球繁育的研究进展.[J]仲恺农业技术学院学报,2002,15(2):66-69
[18]汪有良,南京百合快速繁殖[J].江苏林业科技,1995,22(1):35-43.
[19]陈心启,许介眉,梁松筠等.中国植物志(第十四卷)[M].北京:科学出版社,1980.118-157.
[20]赵祥云,陈新露,王树栋.等.秦巴山区野生百合资源研究初报[J].西北农业大学学报.1990,18(4):80-84
[21]郎楷永.中国的百合[J].科技导报,2003,(7):24-37.
[22]张延龙,牛立新,梁建丽等.秦岭野生百合生长发育的研究[C].园艺学进展第六辑,2004,622-625.
[23]中国农学会遗传资源学会.中国作物遗传资源[M].北京:中国农业出版社,1994.1163-1164.
[24]彭隆金.百合杂交研究.植物引种驯化集刊[C].第八集.北京:科学出版社,1993.99-100.
[25]史跃林.组培苗的生育环境与调节[J].植物生理学通讯,1990(3).
[26]陈为民,宋为民.卷丹和青岛百合的组织培养及植株分化[J].植物生理学通讯,1982,(2):35-36
[27]黄济明.百合的组织培养和试管内诱发多倍体试验[J].园艺学报,1983, 10(2):125-128
[28]杨柏云,罗丽萍,蔡奇英.切花百合组织培养的研究闭.南昌大学学报(理科版),2000,24(4):323-331
[29]罗丽萍,杨柏云,章敏华等.百合组织培养阴.中草药,2001,32(7):640-64
[30]宋建英,叶建仁.‘马可’百合组织培养研究[J].南京林业大学学报,2006,30(4):73-76
[31]马惠,郭扶兴,周俊彦,等.百合叶片愈伤组织的诱导和植株再生[J].植物生理学通讯,1992,4(28):284-287
[32]卢其能.龙牙百合的组织培养和快速繁殖[J].江西农业学报,2002,14(4):43-46
[33]马纯艳.有斑百合叶片的组织培养[J].沈阳师范学院学报(自然科学版),2002,20(3):219-21
[34]王志,朱万芹,李凤沈.新普百合叶片的组织培养[J].山东农业科学,2005,(5):10-15
[35]洪艳华,殷广峰,张立军.百合脱毒及病毒检测技术研究进展[J].沈阳农业大学学报,2003,34(3):225-227
[36] Mori K,Hosokawa D,Watabe M.Studies on multiplieation and distribution of virushe in plants by use of fluoreseent antibody technique.I. Multiplicaion and distribution of viruses in shoot apices[J]. Ann phytopath Soc Japan,1982,48:433-443
[37] Walkey DGA,Production of virus-free plant.In:Tseng C K ed. Applied plant virology[M].New York:Science Press,1991:270-290
[38]赵祥云,程廉,邢尤美等.百合珠芽组培及脱毒研究[J].园艺学报,1993,20(3):284-288
[39]李浚明.植物组织培养教程[M]北京:中国农业大学出版社,2002,14-17
[40]李耿光,张兰英.庸香百合茎段组织培养快速繁殖[J].云南植物研究,1983,5(3):327-33
[41]陈小兰,胡琼花,王红霞.金百合的离体快速繁殖[J].植物生理学通讯,2000,36(4):334
[42]席梦丽,王节萍,章静娟,等.宜兴百合脱毒技术[J].江苏农业学报,2001,17(l):49-51
[43]王红霞,胡琼花,陈小兰.通江百合的组织培养[J].植物生理学通讯,2O00,36(2):132
[44]郭海滨,雷家军.卷丹百合鳞片及珠芽组织培养[J].中国农学通报,2006,22(2):72-74
[45]诸云霞,张永青,杨红娟等.百合花药愈伤组织诱导中的基因型差异[J].上海农业学报,2002,18(3):17-20
[46] SharpWR,RashinRS,SomerHE.HaploidinLilium[J].Phytomorphology,1971,(21):331-337
[47]谷祝平,郑国錩.从百合花粉诱导花粉植株的研究[J].植物学报,1982,24(1):28-31
[48]刘雅莉,张剑侠,潘学军.东方百合‘索邦’的花器官培养与快速繁殖[J].西北植物学报,2004,24(12):2350-2354
[49]王月芬,奚元龄,陆维忠等.兰州百合体细胞组织培养的培养基成分[J].江苏农业学报,1987,3(2):18-24
[50]黄惠英.东方百合的离体培养[J].甘肃农业大学学报,2000,35):450-453
[51]龙春林,程治英,王俐等.兰州百合器官离体培养外植体位置效应观察[J].云南植物研究,2004,26(2):221-225
[52]王蒂.植物组织培养[M].北京:中国农业出版社,2004:36-38,261-263
[53]张文种.不同激素配比对庸香百合鳞茎芽诱导的影响[J].亚热带植物学,2002,3l(l):2l-24
[54]王林,杨万年.宜昌百合小鳞茎诱导的初步研究[J].氨基酸和生物资源,2005,27(3):7-8
[55]李倩中,刘晓青,陈尚平.不同浓度IBA和6-BA对百合组织培养的影响[J].南京农专学报,2003,19(2):43-45
[56]孙君社,方晓华.植物激素对百合鳞片愈伤组织生长的影响[J].中国农业大学学报,2001,6(2):58-61
[57]潘锦旭,邢震,郑维列.西藏卓马百合组织培养技术研究[J].中国生态农业学报,2002,10(2):26-28
[58]冷肖荀,王青华.外植体和培养因子对百合不定芽诱导的影响[J].河北科技,2000,(l):l-2
[59]张德华,焦成瑾,王亚馥等.小麦体细胞胚胎发生及其与合子胚比较[J].兰州大学学报,30(增刊):55-59
[60]崔凯荣,戴若兰.植物体细胞胚发生的分子生物学[M].北京:科学出版社,2000:21-25
[61]黄学林,李筱菊.高等植物组织培养离体培养的形态建成及其调控[M].北京:科学出版社,1995:18-30
[62]邝哲师,周丽侬,马雪荡,等.香蕉和荔枝体胚发生两种不同途径的研究[J].农业生物技术学报,1998,6(3):21-22
[63]郊艳红,熊庆娥.植物体细胞胚胎发生的研究进展[J].四川农业大学学报,2003,2(l):30(增刊):55-59
[64]王亚馥,徐庆,刘志学.红豆草细胞悬浮培养中体细胞胚的形成[J].实验生物学报,1990,23(3): 369-373
[65]周俊彦.植物体细胞在组织培养中产生的胚状体I.植物体细胞的胚状体发生[J].植物生理学报,1981,7(4):389-396
[66]崔凯荣,任红旭,邢更妹,等.构祀组织培养中抗氧化酶活性与体细胞胚胎发生相关性的研究[J].兰州大学学报(自然科学版),1998,34(3):93-99
[67]周俊彦,郭扶兴.细胞分裂素类物质在植物体细胞胚发育中的作用[J].植物生理学通讯,1996,32(4):247-253
[68]王耀芝,丁惠宾.被子植物胚胎学实验图谱,烟草胚的发育[M].兰州大学出版社,1992:92-94
[69]李景原,李大卫,毛使民.山丹的组织培养和再生鳞茎的形态解剖学研究[J].河南师范大学学报(自然科学版),1995,23(1):102-104
[70]罗凤霞,徐桂花,金丽丽等.新铁炮百合微繁的研究[J].沈阳农业大学学报,2000,31(3):254-257
[71]杨柏云,罗丽萍,蔡奇英.切花百合组织培养的研究[J].南昌大学学报(理科版),2000,24(4):323-32
[72]刘菊华,金志强,徐壁玉.龙牙百合的植株再生与遗传转化[J].分子植物育种,2003,1(4):465-474
[73]杜捷,王刚,幸亭泰等.兰州百合继代培养过程中的染色体变异.西北师范大学学报,2003,39(2): 61-65
[74]王爱勤,周歧伟,何龙飞等.百合试管结鳞茎的研究[J].广西农业大学学报,1998,17(1):7l-75
[75]傅玉兰,何风群.影响百合试管鳞茎增殖因素的研[J].安徽农业大学学报,2001,28(2):179-181
[76]方少忠,蔡宣梅,林真等.CEPA和KT对东方百合仔球复壮的影响[J].江西农业大报,2004,26(6) :893-895
[77]张延龙,梁建丽,牛立新.东方百合试管鳞茎膨大的研究[J].西北农林科技大学学报,2006,34(6): 75-78
[78] Joung Hyang Young and Sung Moonsuk.In vitro Propagation of L.oriental hybrid'Casa Blanca'and L.leichtlinii var.tigrinum as influenced by growth regulators and cultural explant[J].Journal of Agriculture Science.1995,37(1):378-383.
[79] Lim S,Seon J H,Paek K Y,etal.Development of pilotscale process for mass production of Lilium bulblet in vitro[J].Acta Hort.1998,461:237-241.
[80] Nhut D T.Micropropagation of lily(L,longiflorum)via in vitro stem node and pseudo bulblet culture[J].Plant Cell Reports,1998,17:913-916.
[81] Dennis P,Stimart,Peter D.Ascher and Harold F.Wildins.Overcoming dormancy in L.longi-florum bulblets produced in tissue culture[J].Hort.Sci.1982,107(6):1004-1007.
[82] Kato T.Physiological Studies on bulb formation and dormancy in the onion plant.VLThe relation between the metabolism of ribberellin and nucleic acid and bulbing phenomenon[J].Jap Soc Hort Sci,1965,34:305.
[83] Levy D,Kedar N,karaeinque K.Effect of the plon on bulbing of onion under non-inductivephotoperiod[J].Hort Science,1973,8:228.
[84] Levy D.Kedar N.Effect of ethrel on growth and bulb initiation in onion [J].HoctScience, 1970,5:80.
[85]田长恩.多胺在离体培养的植物组织形态建成中的作用[J].植物生理学通讯,1992,28(3):230-232.
[86] Tanimoto S.Matsubara Y.Stimulating effect of spermine on Lmlblet formation in bulb-scale Segments of Lilium longiflorum[J].Plant Cell Reports.1995,15:297-300.
[87] English J.Bonner j,Snit H.The wound hormones of plants.The isolation of a crystalline active substance[J].Proc Natl Acad Sci USA,1939,25:323-329.
[88] Marinangeli P,Devalis R,Deelmastro S.Curvetto N.Stimulation of blllblet differentiation by traumatic acid in Lilium blllb-scale segments cultured in vitro[J].Biocell,1995,19:189-195.
[89] Marinangeli P.Curvtto N Bulb quality and traumatic acid influence bulblet formation from scaling in Lilium species and hybrids[J].Hort Science.1997,32(4):739-741.
[90] Marinangeli P,Delmastro S,Curvtto N.Influence of DB-cAMP adenosine forskolin alld traumatic acid on invitro bulbing of Lilium longiflorum[J].Hort Science.1998,33(1):l5l-152.
[91] Takayama S and Misawa M.Differentiation in Lilium bulb grown in vitro,effect of various cultural conditions[J].Physiologia Plantarum.1979,46(2):184-190.
[92]王爱勤,周歧伟,何龙飞等.百合试管结鳞茎的研究[J].广西农业大学学报,1998,17(l):71-75.
[93] Lim S,Seon J H,Paek K Y,etal.Development of pilotscale process for mass production of Lilium bulblet in vitro[J].Acta Hort,1998,461:237-241.
[94]阮少宁,杨华,梁一池等.香水百合组织培养的试验研究[J].福建林学院学报,2001,21(2):142-145.
[95]江如蓝.多效唑对观赏植物的生物学效应及其在植物组织培养上的应用[J].仲恺农业技术学院学报,2000,13(1):50-57.
[96]唐前瑞,谭艳云,于晖.多效唑对非洲菊试管苗生根的影响[J].湖南农业大学学报,22(1):29-32.
[97]徐启江,李玉花,陈典.PP333对分蘖洋葱试管苗增殖和生根的影响[J].植物生理学通讯,40(3):314-316.
[98]朱道圩,张会丽,王锦等.多效唑对大花高代组培苗生长的效应[J].植物生理学通讯,2006,42(2): 232-234.
[99]徐琼,彭志云.多效唑与蔗糖对不同阶段彩色马蹄莲组培苗质量的影响[J].农业网络信息,2006,6:165-167.
[100]王家福,陈振光.百合快速繁殖条件的优化[J].福建农业大学学报,1999,28(2):152-156.
[101] Park NB.Effect of temperature,scle position and growth regulators on the bulblet formation and growth during scale propagation of Lilium[J].Acta Horticulture,1996,414:257-262.
[102] Jeong JH.In vitro propagation of bulb scale section of several korean native lilies[J].Acta Horticulture,1996,414:269-276.
[103]金淑梅,杨利平,吕品等.细叶百合中内源激素的变化[J].东北林业大学学报,2005,33(1):20-22.
[104] Langens-Gerrits-Metal.Bulb growth in Lily regenerated in vitro [J].Acta Horticulture, 1997, 430:267-273.
[105]曹孜义,李唯,王希圣.环境因素和ABA对葡萄试管苗气孔开闭的影响[J].植物生理报,1993,(19)4: 372.
[106]丰锋.提高试管苗移栽成活率的技术研究[J].中国南方果树,2001,30(4):63-64.
[107]蒲秀琴,薛寒清.百合组培苗移栽前的壮苗技术研究[J].青海农林科技,2007,1:59-60.
[108]韦三立.花卉无土栽培[M].北京:中国林业出版社,2001.
[109]夏文孝,郭达初,金敏信.环境条件及基质对试管苗移栽成活率的影响[J].浙江农业学报,1994,6,(3): 171-175.
[110]王凤兰,周厚高等.麝香百合抗热性生理生化指标及综合评价初探[J].广西植物2005,28(2): 261-264
[111]王凤兰,周厚高等.麝香百合基因型抗热性差异及其早期鉴定[J].湖北农业科学,2007,46(2): 253-255
[112]王凤兰,周厚高,黄玉源.4个新铁炮百合品系幼苗的抗热指标测定[J].仲恺农业技术学院学报,2003,16(2):38-42
[113]王凤兰,周厚高,黄子峰,等.新铁炮百合叶片抗热性形态指标初探[J],湖北农学院学报,2004,24(2):102-105.
[114]周斯建,义鸣放,穆鼎,高温胁迫下铁炮百合幼苗形态及生理反应的初步研究[J].园艺学报.2005,32(1):145～147
[115]张施君,周厚高,潘文华,余卓玲.新铁炮百合的耐热性生理初步研究[M].雷建军主编:园艺学进展(第五辑).广州:广州出版社,2002.665～669
[116]王光耀,刘俊梅,张仪,等.菜豆四个不同抗热性品种的气孔特性[J].农业生物技术学报,1999,7(3):267-270.
[117]潘宝贵,王述彬,刘金兵.高温胁迫对不同辣椒品系苗期光合作用的影响[J].江苏农业学报,2006,22(2):137-140.
[118]彭永宏,章文才.猕猴桃叶片耐热性指标研究[J].武汉植物学研究,1995,13(I):70-74.
[119]叶小梅,卢忠华,蔡丽萍,等.4种引进月季品种叶片耐热性生理生化指标初探[J].广东林业科技,2007,23(1):80-82.
[120]王向阳,王淑俭,彭文博,等.高温对小麦幼苗生理特性影响的初步研究[J].河南农业大学学报,1992,26(1):9-15.
[121]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究.II.叶片水分和蛋白质代谢与耐热性[J].福建农业大学学报,1996,25(4):490-493.
[122]陈火瑛,江隆植,张建华.萝卜抗热性鉴定技术[J].上海蔬菜,1992,(3):15-16.
[123]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社. 1995.199-215.
[124]刘存德.栽培植物的抗性鉴定[J].植物生理学通讯,1957,(3):67-71.
[125]陈立松,刘星辉.高温对桃和抽细胞膜透性和光合色素的影响[J].武汉植物学研究,1997,15(3):233-237.
[126] Viering A,Nguyen H T,Heat-shock gene expression in diploid wheat genotypes differing in thermal tolerance[J]. Crop Sci,1992,32:370-377.
[127]李绍鹏.芒果叶片抗旱性鉴定的初步研究[J].热带作物研究,1993,(4):56-59.
[128]罗少波,李智军,周微波等.大白菜品种耐热性的鉴定方法[J].中国蔬菜,1996,(2):16-18.
[129]杨丽薇,王景义,梁惠芳等.早熟大白菜耐热性鉴定技术[J].北方园艺,1996,(6):5-6.
[130]马德华,庞金安,霍振荣等.黄瓜对不同温度逆境的抗性研究[J].中国农业科学,1999,32(5):28-35.
[131]黄莺,赵致.杂交玉米品种抗早性生理指标及综合评价初探[J].种子,2001,(1):12-14.
[132]肖顺元,赵大中.柑桔叶片耐热的生理生化指标[J].果树科学,1990,(7):217-220.
[133]关义新,戴俊英,陈军等.土壤千早下玉米叶片游离脯氨酸的累积及其与抗早性的关系[J].玉米科学,1996,4(1):43-58.
[134] Tahir M.Signh M.Assessment of screening techniques for heat tolerance in wheat[J]. Crop Sci., 1993,33:740-744.
[135] Bery J Aetal. Respones of macrophytes to temperature. In:Physiological Plant Ecology1. Respones to Physical Environment[J]. Heidelberg: Springer Verlag,1981,277-288.
[136]邹琦.小麦高温伤害与高温适应[J].植物学报,1988,30(4):388-395.
[137]谷崇光,陈文,许启新等.电导百分率法测定黄瓜耐热性[J].上海蔬菜,1991,(I):33-34.
[138]罗少波,周微波,罗剑宁,等.黄瓜品种耐热性强度鉴定方法比较[J].广东农业科学,1997,(6):23-24
[139]陆世钧,乔炳根.早熟大白菜耐热性与EC值关系初探[J].上海蔬菜,1990,(2):35.
[140]司家钢,孙日飞,吴飞燕等.高温胁迫对大白菜耐热性相关生理指标的影响[J].中国疏菜,1995,(4):4-6,15.
[141]曾韶西,王以柔,刘鸿先,低温下黄瓜幼苗子叶硫氢基(SH)含量变化与膜脂过氧化[J].植物学报,1991,33(1):50-54.
[142]李成琼,宋洪元,雷建军等.甘蓝耐热性鉴定研究[J].西南农业大学学报,1998,20(4):198-201.
[143]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究.I.根系生理活动与耐热性[J].福建农业大学学报,1996,25增刊):490-493.
[144]刘维信,曹寿椿.高温对不结球大白菜细胞膜透性过氧化物酶活性等的影响[J].南京农业大学学报,1992,15(3):115-117.
[145]姚元干,石雪晖,杨建国等.辣椒叶片耐热性生理生化指标探讨[J].湖南农业大学学报,1998,24(2):119-122.
[146] Martinean J R,Specht J E.Temperature tolerance in soybeans[J].Crop Science,1979,19:75-81
[147]陈少裕.膜脂过氧化与植物逆境胁迫[J]植物学通报,1989,6(4):211-217.
[148]周人纲,樊志和,李晓芝等.热锻炼对小麦叶片细胞膜及有关酶活性的影响[J].作物学报,1995,(5):568-572.
[149]叶陈亮,柯玉琴,陈伟.大白菜耐热性的生理研究III.酶性和非酶性活性氧清除能力与耐热性[J].福建农业大学学报,1997,26(4):498-501.
[150]吴国胜,曹婉虹,王永健等.细胞膜热稳定性及保护酶与大白菜耐热性的关系[J].园艺学报,1995,22:353-358
[151]李合生,孙群.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999.258-263
[152]周厚高,江如蓝,王凤兰等.百合[M].广州:广东科技出版社,2003.15-17