中国亚热带海域超微型浮游生物多样性研究
|
摘要
本论文通过光合色素分析技术和小亚基核糖体RNA基因序列分析技术(ARDRA和DGGE)研究我国亚热带近海典型海区(包括陆架、河口、港湾、上升流区域等)超微型浮游植物光合色素组成和时空变化以及超微型真核浮游生物群落组成和多样性的时空变化,研究海域包括台湾海峡南部海域(2004年7月)、珠江口及南海北部(2004年2月和2004年7月)以及厦门海域(2003年3月、2003年5月、2003年8月、2003年12月、2004年2月、2004年5月和2004年9月),取得的主要结果如下:
台湾海峡南部海域特征光合色素指示了九种超微型浮游植物类群在台湾海峡南部海域的分布,不同类群表现出对上升流过程的不同响应。超微型浮游植物的多数类群与营养盐呈显著正相关,受营养盐补充刺激生长;两种原核浮游植物蓝藻与原绿球藻的分布受营养盐限制。超微型浮游植物的类群组成具有明显垂直分布特征,蓝藻对CHLA生物量贡献随深度增加而下降,硅藻、青绿藻或定鞭金藻在真光层底部具有更高比重。构建了台湾海峡南部四个18S rDNA文库。在获得序列中,Novel Alveolates(NA)是基因文库中克隆丰度最高的类群;甲藻居第二;青绿藻是遗传文库中重要组成,存在与最小真核生物Ostreococcus sp.极相似的序列。聚类分析将超微型真核浮游生物遗传结构分为不同类型,超微型真核浮游生物遗传结构的水平分布空间变化明显,垂直分布差异较小。大量OTUs(Operational Taxonomy Units)的存在提供了各种环境条件的指示;在上升流海区的样品中,都存在着可能可以作为近岸上升流和台湾浅滩地形上升流指示的OTUs。
厦门海域特征光合色素的分布表明硅藻、青绿藻、隐藻和绿藻是厦门海域超微型浮游植物的主要类群。超微型浮游植物类群结构的季节性变化较为明显,冬季叶绿素a最低,以硅藻、隐藻和青绿藻为主;夏季叶绿素a浓度最高,以硅藻为唯一的优势类群。超微型浮游植物类群结构的空间变化远小于季节性变化。在冬季与春季,超微型浮游植物类群主要受到温盐的影响,在夏、秋季,部分浮游植物类群,特别是青绿藻受磷酸盐影响明显。18S rDNA文库序列分析表明具有较
Photosynthetic pigments analysis and molecular techniques including ARDRA and DGGE for analyzing small subunit ribosomal RNA(SSU rRNA or 18S rRNA) gene were applied to study temporal and spatial distributions of pigment composition and genetic diversity of ultra-plankton community in subtropical waters of China Seas, including the upwelling regions of southern Taiwan Strait(July, 2004), the entrance of Pearl River(Feb, 2004), the continental shelf along northern South China Sea(Feb, 2004 and July, 2004), and Xiamen Bay (Mar, 2003; May, 2003; Aug, 2003; Dec, 2003; Feb, 2004; May, 2004; Sept, 2004). Main conclusions are as the following : The southern Taiwan Strait, Indicated by diagnostic pigments, ultraphytoplankton groups responded to upwelling events by different distribution patterns. Most significant changes on vertical distribution were that the proportion of Cyanobacteria to total CHLA biomass decreased with depth, while Bacillariophyta, Prasinophyceae or Haptophyta increased with depth . Most of groups were significantly positive correlated to nutrients, suggesting that those groups are likely to be stimulated by nutrients coupled by upwelling,while Cyanobacteria and Prochlorophyta were negative correlated to nutrients.
Four 18S rDNA libraries for eukaryotic ultraplankton in the southern Taiwan Strait were constructed. Sequences which were similar to NA and Dinophyceae were very abundant. Certain sequences which were similar to smallest eukaryote, Ostreococcus sp., were an important component in screened clones. Clustering and NMDS analysis revealed that vertical variations among most stations were lower than that between stations. Amounts of OTUs provided indicators for different water masses, such as upwelling.
Xiamen Bay Indicated by diagnostic pigments, Bacillariophyta, Prasinophyceae, Cryptophyta and Chlorophyceae were dominant groups of ultraphytoplankton, while Prochlorophyta was not detected all time. Community structure was kept stable in same cruise, however, seasonal variations were prominent. Average CHLA concentration in February of 2004 was lowest, with community structure dominated by Bacillariophyta, Cryptophyta and Prasinophyceae. The highest CHLA concentration appeared in August of 2003, while Bacillariophyta were only dominating groups. In cold season, temperature and salinity determined the distributions of ultraphytoplanktons, while in warm season, Prasinophyceae were
台湾海峡南部海域特征光合色素指示了九种超微型浮游植物类群在台湾海峡南部海域的分布,不同类群表现出对上升流过程的不同响应。超微型浮游植物的多数类群与营养盐呈显著正相关,受营养盐补充刺激生长;两种原核浮游植物蓝藻与原绿球藻的分布受营养盐限制。超微型浮游植物的类群组成具有明显垂直分布特征,蓝藻对CHLA生物量贡献随深度增加而下降,硅藻、青绿藻或定鞭金藻在真光层底部具有更高比重。构建了台湾海峡南部四个18S rDNA文库。在获得序列中,Novel Alveolates(NA)是基因文库中克隆丰度最高的类群;甲藻居第二;青绿藻是遗传文库中重要组成,存在与最小真核生物Ostreococcus sp.极相似的序列。聚类分析将超微型真核浮游生物遗传结构分为不同类型,超微型真核浮游生物遗传结构的水平分布空间变化明显,垂直分布差异较小。大量OTUs(Operational Taxonomy Units)的存在提供了各种环境条件的指示;在上升流海区的样品中,都存在着可能可以作为近岸上升流和台湾浅滩地形上升流指示的OTUs。
厦门海域特征光合色素的分布表明硅藻、青绿藻、隐藻和绿藻是厦门海域超微型浮游植物的主要类群。超微型浮游植物类群结构的季节性变化较为明显,冬季叶绿素a最低,以硅藻、隐藻和青绿藻为主;夏季叶绿素a浓度最高,以硅藻为唯一的优势类群。超微型浮游植物类群结构的空间变化远小于季节性变化。在冬季与春季,超微型浮游植物类群主要受到温盐的影响,在夏、秋季,部分浮游植物类群,特别是青绿藻受磷酸盐影响明显。18S rDNA文库序列分析表明具有较
Photosynthetic pigments analysis and molecular techniques including ARDRA and DGGE for analyzing small subunit ribosomal RNA(SSU rRNA or 18S rRNA) gene were applied to study temporal and spatial distributions of pigment composition and genetic diversity of ultra-plankton community in subtropical waters of China Seas, including the upwelling regions of southern Taiwan Strait(July, 2004), the entrance of Pearl River(Feb, 2004), the continental shelf along northern South China Sea(Feb, 2004 and July, 2004), and Xiamen Bay (Mar, 2003; May, 2003; Aug, 2003; Dec, 2003; Feb, 2004; May, 2004; Sept, 2004). Main conclusions are as the following : The southern Taiwan Strait, Indicated by diagnostic pigments, ultraphytoplankton groups responded to upwelling events by different distribution patterns. Most significant changes on vertical distribution were that the proportion of Cyanobacteria to total CHLA biomass decreased with depth, while Bacillariophyta, Prasinophyceae or Haptophyta increased with depth . Most of groups were significantly positive correlated to nutrients, suggesting that those groups are likely to be stimulated by nutrients coupled by upwelling,while Cyanobacteria and Prochlorophyta were negative correlated to nutrients.
Four 18S rDNA libraries for eukaryotic ultraplankton in the southern Taiwan Strait were constructed. Sequences which were similar to NA and Dinophyceae were very abundant. Certain sequences which were similar to smallest eukaryote, Ostreococcus sp., were an important component in screened clones. Clustering and NMDS analysis revealed that vertical variations among most stations were lower than that between stations. Amounts of OTUs provided indicators for different water masses, such as upwelling.
Xiamen Bay Indicated by diagnostic pigments, Bacillariophyta, Prasinophyceae, Cryptophyta and Chlorophyceae were dominant groups of ultraphytoplankton, while Prochlorophyta was not detected all time. Community structure was kept stable in same cruise, however, seasonal variations were prominent. Average CHLA concentration in February of 2004 was lowest, with community structure dominated by Bacillariophyta, Cryptophyta and Prasinophyceae. The highest CHLA concentration appeared in August of 2003, while Bacillariophyta were only dominating groups. In cold season, temperature and salinity determined the distributions of ultraphytoplanktons, while in warm season, Prasinophyceae were
引文
1. Acinas S.G., Rodriguez-Valera F., Pedros-Aliq C., Spatial and temporal variation in marine bacterioplankton diversity as shown by RFLP fingerprinting of PCR amplified 16S rDNA[J]. FEEMS Microbiology Ecology, 1997,24:27~40.
2. Adachi, M., Sako, Y., Ishida,Y. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions[J]. J.Phycol, 1996,32:424~432.
3. Ahel, M., R.G. Barlow, R.F.C. Mantoura, Effect of salinity gradients on the distribution of phytoplankton pigments in a stratified estuary[J]. Mar. Eco. Prog. Ser., 1996. 143: 289-295.
4. Amann, R.I., Ludwig, W., Schleifer, k.H., Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiology Review, 1995, 59:143~169.
5. Andersen R. A., Biology and systematics of heterokont and haptophyte algae [J]. J Bot. 2004, 91: 1508-1522.
6. Andersen, R.A., Bidigare, R.R., Keller, M.D., Latasa, M. A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific oceans[J]. Deep-Sea Res. II, 1996, 43:517–537.
7. Anderson, RA, Saunders, GW, Paskind, MP, et al. Ultrastructure and 18S ribosomal-RNA gene sequence for Pelagomonas calceolata genet sp. nov and the description of a new algal class, the Pelagophyceae classis nov[J]. J Phyco.1993.29:701-715.
8. Ansotegui, A., Trigueros, J. M., Orive, E. The use of pigment signatures to assess phytoplankton assemblage structure in estuarine waters[J]. Estuarine Coastal and Shelf Sci. 2001, 52:689-703.
9. Anton A.I., Martinez-Murcia A.J., Rodriguez-Valera F., Sequence diversity in the 16S-23S intergenic spacer region (ISR) of the rRNA operons in representatives of the Escherichia coli ECOR collection[J]. J Mol Evo. 1998, 47: 62~72.
10. Azam F, Fenchel T, Field JG, The ecological role of water column microbes in the sea . Mar Ecol Prog Ser, 1983, 10: 257-263.
11. Babin M., Morel A., Gentili B., Remote sensing of sea surface Sun-induced chlorophyll fluorescence: consequences of natural variations in the optical characteristics of phytoplankton and the quantum yield of chlorophyll a fluorescence[J], J. Remote Sens., 1996,17 (1): 2417–2448.
12. Baldauf, S.L. The deep roots of eukaryotes[J]. Science, 2003, 300: 1703–1706.
13. Barlow, R.G., Cummings, D.G., Gibb, S.W. Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC[J]. Mar. Eco. Pro. Ser., 1997a, 161: 303-307.
14. Barlow, R.G., Mantoura, R.F.C., Cummings, D.G. Pigment chemotaxonomic distributions of phytoplankton during summer in the western Mediterranean[J]. Deep-Sea Res II. 1997b, 44(3-4): 833–850.
15. Barlow, R.G., Mantoura, R.F.C., Gough, M.A. Pigment signatures of the phytoplankton composition in the northeastern Atlantic during the 1990 spring bloom[J]. Deep-Sea Res II,1993, 40:459–477.
16. Barlow, R .G., Mantoura R. F.C., Peinert R., Miller A.E. J., Fileman T.W., Distribution, sedimentation and fate of pigment biomarkers following thermal stratification in the western Alboran Sea[J], Mar. Eco. Pro. Ser., 1995, 125,279–291.
17. Bianchi, T.S., Findlay, S., Dibb, J. Early diagenesis of plant pigments in Husdon River sediments[J]. Estuarine Coastal and Shelf Science. 1993, 36: 517-527.
18. Bianchi, T.S., Findlay, S., Fontvieille, D. Experimental degradation of plant materials in Hudson River sediments. I. Heterotrophic transformations of plant pigments[J]. Biogeochemistry, 1991, 12: 171-187.
19. Bianchi, T.S., Johansson, B., Elmgren, R. Breakdown of phytoplankton pigments in Baltic sediments, effects of anoxia and loss of deposit-feeding macrofauna[J]. J Exp Mar Bio Eco, 2000, 251:161-183.
20. Bianchi, T.S., Kautsky, L., Argyrou, M. Dominant chlorophylls and carotenoids in macroalgae of the Baltic Sea (Baltic Proper): their use as potential biomarkers[J]. Sarsia, 1997, 82:55–62.
21. Bidigare R.R., Kennicutt II M.C., Brooks J.M., Rapid determination of chlorophylls and their degradation products by high-performance liquid chromatography[J], Limnol. Oceanogr., 1985,30 (2): 432–435.
22. Bidigare, R.R., Kennicutte, M.C., Keeney-Kennicutt, A. M. Isolation and purification of chlorophylls a and b for the determination of stable carbon and nitrogen isotope compositions[J]. Anal. Chem., 1991, 63:130-133.
23. Bidigare, R.R., Marra, J., Dickey, T.D. Evidence for phytoplankton succession and chromatic adaptation in the Sargasso Sea during spring 1985. Mar. Ecol. Prog. Ser[J], 1990, 60: 113–122.
24. Biegala IC, Not F, Vaulot D, Simon N. 2003. Quantitative assessment of picoeucaryotes in the natural environment using taxon specific oligonucleotide probes in association with TSA-FISH (Tyramide Signal Amplification - Fluorescent In Situ Hybridization) and flow cytometry[J]. Appl. Environ. Microbiol. 69: 5519-5529.
25. Bowers, H. A., T. Tengs, Glasgow H. B., Jr., Burkholder J. M., Rublee P. A., and Oldach D. W. Development of realtime PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates[J]. Appl. Environ. Microbiol. 2000. 66:4641-4648.
26. Boynton,W.R, Garbaer H, R. Summers, Inputs, transformations and transport of nitrogen and phosphorus in Chesapeake Bay and selected tributaries[J] . Estuaries, 1995.18: 285-314.
27. Bricaud, A., Morel, A., Babin, M. Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (Case 1) Waters: analysis and implications for bio-optical models[J]. J. Geophys. Res., 1998, 103 (C13): 31033–31044.
28. Bricelj,V.M. and Lonsdale, D.J. Aureococcus anophagefferens : Causes and ecological consequences of brown tides in U.S. mid-Atlantic coastal waters[J]. Limnol. Oceanogr. 1997, 42: 1023–1038.
29. Buck, K. R., F. P. Chavez, and L. Campbell. Basin-wide distributions of living carbon components and the inverted trophic pyramid of the central gyre of the North Atlantic Ocean, summer 1993[J]. Aquat. Microb. Ecol. 1996.10: 283–298.
30. Burkill P.H., Mantoura R.F.C., Owens N.J.P. 1993. Biogeochemical cycling In the northwestern Indian Ocean: a brief overview[J]. Deep-Sea Res Ⅱ,40(3): 643~649.
31. Campbell, L., H. A. Nolla, and D. Vaulot, The importance of Prochlorococcus to community structure in the central North Pacific Ocean[J]. Limnol. Oceanogr. 1994,39:954–961.
32. Caron, D. A. (1991) Evolving role of protozoa in aquatic nutrient cycles[M]. In Reid, P. C., Turley, C. M. and Burkhill, P. H. (eds), Protozoa and their Role in Marine Processes. Springer Verlag, Berlin, pp. 387–415.
33. Caroppo C,The contribution of picophytoplankton to community structure in a Mediterranean brackish environment[J]. J Plank Res, 2000 ,22(2):381–397.
34. Casamayor, E.O., Sch?fer, H., Ba?eras, L., Pedrós-Alió, C ., Muyzer, G. Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: a comparison of microscopy and denaturing gradient gel electrophoresis[J]. Appl. Environ. Microbiol. 2000, 66:499–508.
35. Chen, F., Dustman, W.A., Hodson, R.E. Detection of toluene dioxygenase gene and gene expression in Pseudomonas putida FL in a toluene exposed seawater using in situ PCR and hybridization[J]. Hydrobiologia. 1999, 401:131-138.
36. Chen, F., Jose, M.G., Wendy, A.D., Mary, A.M., Robert, E.H. In situ Reverse tanscription: an approach to characterize genetic diversity and activities of prokaryotes[J]. Appl. Environ. Microbiol. 1997, 63(12): 4907~4913.
37. Chen, F., Wendy, D., Mary, A.M., Robert, E.H. In situ PCR methodologies for visualizatioon of microscale genetic and taxonomic diversities of prokaryotic communities[J]. Molecular Microbial Ecology Manual. 1998: 1~17.
38. Claereboudt M.R., Cote J., Bonardelli J.C.Seasonal variation abundance and size structure of phytoplankton in Baiedes chaleurs,southwestern Golf of St. Lawrence, in relation to physical oceanographic conditions[J]. Hydrobiologia, 1995. 306(2): 147~157.
39. Claustre, H., The trophic status of various oceanic provinces as revealed by phytoplankton pigment signatures[J]. Limnol. and Oceanogr,. 1994.39(5): 1206-1210.
40. Claustre, H., Marty, J.C. Evaluation of the utility of chemotaxonomic pigments as a surrogate for particulate DMSP[J]. Limnol. Oceanogr. 2001, 46(4): 989-995.
41. Corey, D.L., Bianchi, T.S., Santschi, P.H. Cross-shelf changes in phytoplankton community composition in the Gulf of Mexico (Texas shelf/slope): the use of plant pigments as biomarkers[J]. Conti. Shelf. Res. 1999, 19: 1-21.
42. Courties, C. Vaquer, A., Troussellier, M., Lautier, J., Chre′tiennot-Dinet, M-J., Neveux, J., Machado, M. C. & Claustre, H., Smallest eukaryotic organism[J]. Nature, 1994.370:255.
43. Coyne, Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard[J], Limnol. Oceanogr.: Methods 3, 2005, 381–391
44. Cullen, D. W., A. K. Lees, I. K. Toth, and J. M. Duncan. Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR[J]. Plant Pathol. 2002.51:281-292.
45. Daugbjerg &. P Henriksen. 2000. Comparative study of. Gymnodinium mikimotoi an. Gymnodinium aureolum, comb. nov. (=Gyrodinium. aureolum) based on morphology, pigment composition. and molecular data[J] J Phycol 36: 394 - 410
46. Daugbjerg, N., and R.A. Andersen. Phylogenetic analysis of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated[J]. Mol. Biol. Evol. 1997. 14:1242-1251.
47. Davis, PG, Caron, DA, Johnson, PW and Sieburth,. J.McN. Phototrophic and apochlorotic components. of picoplankton and nanoplankton[J]. Mar. Ecol. Prog. Ser. , 1985, 21: 15-26
48. Dawson, S. C., and N. R. Pace. Novel kingdom-level eukaryotic diversity in anoxic environments.Proc. Natl. Acad. Sci. 2003.:8324-8329.
49. Dawson, S.C., E.F. DeLong and N.R. Pace Phylogenetic and ecological perspectives on uncultured Crenarchaeota and Korarchaeota[M] In M. Dworkin et al., eds., The Prokaryotes: An Evolving Electronic Database for the Microbiological Community, New York, Springer-Verlag, 2000
50. Delong, E.F., Wickham, G.S., pace, N.R. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells[J]. Science, 1989, 243: 1360~1362.
51. Demers, S., Roy, S., Gagnon, R. Rapid light-induced changes in cell fluorescence and in xanthophyll-cycle pigments of Alexandrium excavatum (Dinophyceae) and Thalassiosira pseudonana (Bacillariophyceae): a photo-protection mechanism[J]. Mar. Ecol. Prog. Ser., 1991, 76: 185–193.
52. Diez, B., Pedro′s-Alio′, C. , Massana, R. Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing[J]. Appl. Environ. Microbiol. 2001a, 67: 2932–2941.
53. Diez, B., Pedrós-Alió, C., Marsh, T L., Massana, R. Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques[J]. Appl. Environ. Microbiol. 2001b, 67:2942–2951.
54. Dragica Radojkovic and Jelena Ku ic , Silver Staining of Denaturing Gradient Gel Electrophoresis Gels[J] , Clinical Chemistry. 2000;46:883-884.
55. Du H. L., Nianzhi Jiao, Yaohua Hu, Yonghui Zeng, Real-Time PCR for Quantification of Aerobic Anoxygenic Phototrophic Bacteria Based on pufM Gene in Marine Environment[J]. Journal of Experimental Marine Biology and Ecology. 2005, 323(2): 95-103.
56. Dugdale, R.C. and J.J. Goering. Uptake of new and regenerated forms of nitrogen in primary productivity[J]. Limnol. Oceanogr. 1967, 12: 196-206.
57. Edgcomb,V.P., Kysela,D.T., Teske,A., de Vera Gomez,A. and Sogin,M.L. Benthic eukaryotic diversity in the Guaymas Basin hydrothermal vent environment[J]. Proc. Natl. Acad. Sci. USA, 2002, 99:7658–7662.
58. Edvardsen, B. and Paasche, E. Bloom dynamics and physiology of Prymnesium and Chrysochromulina[M]. In: Physiological Ecology of Harmful Algal Blooms. NATO ASI Series G, vol.
41. Anderson, D.M., Cembella, A.D., Hallegraeff, G.M. [Eds.], Springer-Verlag, Heidelberg, 1998.pp. 193-208.
59. Eikrem W. and Moestrup . Structural analysis of the flagellar apparatus and the scaly periplast of Chrysochromulina scutellum sp. nov. (Prymnesiopyceae, Haptophyta) from the Skagerrak and the Baltic[M]. Phycologia. 1998, 37: 132-153
60. Everitt, D.A., Wright, S.W., Volkman, J.K., Thomas, D.P., Lindstrom, E.J. Phytoplankton community compositions in the western equatorial Pacific determined from chlorophyll and carotenoid pigment distribution[J]. Deep-Sea Res., 1990, 37: 975-997.
61. Falkowski P.G., LaRoche J., Acclimation to spectral irradiance in algae, J. Appl. Phycol., 27, 8–14.
62. Falkowski, P.G. ,1994. The role of phytoplankton photosynthesis in global biogeochemical cycles[J]. Photosynthesis Research. 1991, 39: 235-258.
63. Ferris, M.J., Ward, D.M. Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis[J]. Appl. Environ.Microbiol., 1997, 63:1375–1381.
64. Finkel Z. V., Physiological basis for environmentally-driven changes in phytoplankton communities[D], 2005, Dissertation, Dalhousie Unnersity, Halifax, Nova Scotia
65. Fisher, S. G., and L. S. Lerman. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with theory[J]. Proc. Natl. Acad. Sci. USA. 1983. 80:1579-1583.
66. Fogg G E. Some comments on picoplankton and its importance in the pelagic ecosystem[J]. Aquat. Microb. Ecol. 1995, 9:33–39.
67. Friedrich A. B., H. Merkert, T. Fendert, J. Hacker, P. Proksch, U. Hentschel, Microbial diversity in the marine sponge Aplysina cavernicola (formerly Verongia cavernicola) analyzed by fluorescence in situ hybridization (FISH)[J], Marine Biology, 1999, 134:461 – 470.
68. Fuhrman. J.A, McCallum,K.,Davia.A.A, Phylogenetic diversity of subsurface marine microbial cimmunities from the Altantic and Pacific oceans[J]. Appl.Environ.Microbiol. 1993.59:1294-1302.
69. Fuller,N. J., Marie D., Partensky F., et al., Clade-specific 16S ribosomal DNA Oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the Red Sea[J]. Appl Environ Microbiol, 2003, 69(5): 2430~2443.
70. Furlong, E., Carpenter S.R. Pigment preservation and remineralization in oxic coastal marine sediments[J]. Geochimica et Cosmochimica Acta, 1988, 52: 87-100.
71. Furuya, K, M. Hayashi, Y. Yabushita,.HPLC Determination of Phytoplankton Pigments Using N,N-Dimethylformamide[J]. J. Oceanogr., 1998. 54,199-203.
72. Furuya, K., Hayashi, M., Yabushita, Y. I. Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures[J]. Deep-Sea Res. II, 2003, 50: 367-387.
73. Galluzzi, L., A. Penna, E. Bertozzini, M. Vila, E. Garces, and M.Magnani. Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate) [J]. Appl. Environ. Microbiol. 2004. 70: 1199-1206.
74. Gao Y-H,Yang XN,Chen X,Wang DZ and Hong LY. Effect of zinic selenium and manganese on the growth of a marine microalga Nannochloropsis oculata (Eusigmatophyceae) [J]. Chin. J. Limnol .Oceanogr., 2002a.20(special issue): 93-101
75. Gao, Y.H., Gao, Y., Jin, H.M., Jiao, N.Z. Extracelluar dissolved organic carbon of a marine nanoplanktonic diatom Chaeltoceros sp.: Influence of daily rhythm, light and temperature[J]. Chin. J. Limnol .Oceanogr., 2002ba, 20: 81-92.
76. Gaul W., A. N. Antia., Taxon-specific growth and selective microzooplankton grazing of phytoplankton in the Northeast Atlantic[J]. J. Mar. Systems., 2001.30: 241-261.
77. Gelsomino, A., Keijzer-Wolters, A.C., Elasas, J.D., Cacco, G. Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. Journal of Microbiological Methods[J]. 1999, 38:1–15.
78. Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., Field, K.G. Genetic diveristy in Sargasso Sea bacterioplankton[J]. Nature, 1990, 345:60~63.
79. Giovannoni, S.J., S. Turner, G.J. Olsen, D.J. Lane, and N.R. Pace. Evolutionary relationships among cyanobacteria and green chloroplasts[J]. J. Bacteriol. 1988, 170:3584-3592.
80. Goericke, R, Welschmeyer, N A. Pigment turnover in the marine diatom Thalassiosira weissflogii. Ⅱ . The 14CO2-labeling kinetics of carotenoids[J]. J. Phycol, 1992, 28: 507-17.
81. Goericke, R., Montoya, J.P. Estimating the contribution of microalgal taxa to chlorophyll a in the field – variations of pigment ratios under nutrient- and light- limited growth[J]. Mar. Ecol. Prog. Ser., 1998, 169: 97–112.
82. Goericke, R., Repeta, D.J. Chlorophylls a and b and divinyl chlorophylls a and b in the open subtropical North Atlantic Ocean[J]. Mar. Ecol. Prog. Ser, 1993, 101: 307–313.
83. Goericke, R., Repeta, D.J. The pigments of Prochlorococcus marinus, The presence of divinyl chlorophyll a and b in a marine prokaryote[J] Limnol. Oceanogr., 1992, 37: 425-433.
84. Gray, M., B. Wawrik, J. Paul, and E. Casper. Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment[J]. Appl. Environ. Microbiol. 2003. 69:5726-5730.
85. Guillou L., Chrétiennot-Dinet M-J, Medlin L.K., Claustre H., Loiseaux-de Go?r S., Vaulot D. Bolidomonas: a new genus with two species belonging to a new algal class, the Bolidophyceae (Heterokonta)[J]. J Phycol .1999a.35: 368-381
86. Guillou L., Moon-van der Staay S.Y., Claustre H, Partensky F., Vaulot D. Diversity and abundance of Bolidophyceae (Heterokonta) in oceanic waters[J]. Appl Environ Microbiol 1999b. 65: 4528-4536
87. Guillou, L., Eikrem, W., Chrétiennot-Dinet, M. J., Le Gall, F., Massana, R., Romari, K., Pedrós-Alió, C. & Vaulot, D. Diversity of picoplanktonic prasinophytes assessed by direct nuclear SSU rDNA sequencing of environmental samples and novel isolates retrieved from oceanic and coastal marine ecosystems[J]. Protist. 2004, 155:193-214
88. Hallegraeff G.M., Jeffrey S.W., 1985, Description of new chlorophyll a alteration products in marine phytoplankton[J], Deep-Sea Res., 32: 697–705.
89. Head, E.J.H., Harris, L.R. Chlorophyll destruction by Calanus grazing on phytoplankton, kinetics, effects of ingestion rate and feeding history, and a mechanistic interpretation[J]. Mar. Ecol. Prog. Ser., 1996, 135:223-235.
90. Head, I.M., Saunders, J.R., Pickup, R.W. Microbial evolution, diversity, and ecology : A decade of ribosomal RNA analysis of uncultivated microorganisms[J]. Microbia. Ecology., 1998, 35: 1–21.
91. Henriksen P., Riemann B., Kaas H., Sorensen H.M., Sorensen H. L., Effect of nutrient-limitation and irradiance on marine phytoplankton pigments, J. Plankton Res., 2002, 24 (9): 835–858.
92. Hess W.R., Weihe A., Loiseaux-de Goer S., Partensky F. and Vaulot D. Characterisation of the single psbA gene of Prochlorococcus marinus CCMP 1375 (Prochlorophyta)[J]. Plant Molecular Biology 27: 1189-1196.
93. Hess, W.H., Partensky, F., Van Der Staay Vaulot, D. Coexistence of phycoerythrin and a chlorophyll a/b antenna in a marine prokaryote[J]. Proc. Nat. Acad. Sci. U.S.A., 1996, 93: 11126~11130.
94. Heukelem L., Lewitus A. J., Kana T.M., Craft N.E., Improved separations of phytoplankton pigments using temperature-controlled high-performance liquid chromatography[J], Mar. Ecol. Prog. Ser., 1994,114: 303–313.
95. Heukelem, L.V., Thomas, S. Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments[J]. J.Chromatogr., 2001, 910: 31- 49.
96. Higgins, H.W., Mackey, D.J. Algal class abundances, estimated from chlorophyll and carotenoid pigments, in the western Equatorial Pacific under El Nino and non-El Nino conditions[J]. Deep-Sea Research I, 2000, 47: 1461–1483.
97. Hodgson, D.A., Wright S. Davies W., N., Mass spectrometry and reverse phase HPLC techniques for the identification of degraded fossil pigments in lake sediments and their application in paleolimnology[J]. J. Paleolimnology 1997, 18: 335-350
98. Huang,B.Q., Hong, H. and Wang, H., 1999, Size-Fractionated Primary Productivity and the Phytoplankton-Bacteria Relationship in the Taiwan Strait[J], Mar. Ecol. Prog. Ser. 183 : 29-38.
99. Hurley, J.P. and D.E. Armstrong., Pigment preservation in lake sediments, a comparison of sedimentary environments in Trout Lake, Wisconsin[J]. J. Fish. Aquat. Sci, 1991,48: 472-486.
100. Janse I, Bok J, and Zwart G. A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis[J]. Journal of Microbiological Methods. 2004, 57:279–281.
101. Jean-Claude M, Jacques C, Marie-Dominique P, Seasonal and interannual dynamics of nutrients and phytoplankton pigments in the western Mediterranean Sea at the DYFAMED time-series station (1991–1999) [J].Deep-Sea Research II,2002, 49 : 1965–1985
102. Jean-Pierre Descy, B. Destasio, G. Gerrish, J. Hood, J.-P. Hurley, A. St. Amand. Pigment ratio and phytoplankton assessment in northern Wisconsin lakes[J], J. Phycol., 2000, 36: 274-286
103. Jeffrey, S.W., Mantoura, R. F. C., Wright, S. W. Phytoplankton Pigments in Oceanography[M]. SCOR, UNESCO Publishing., 1997
104. Jeffrey, S.W., Wright, S.W., Zapata, M. Recent advances in HPLC pigment analysis of phytoplankton[J]. Marine and Freshwater Research, 1999, 50: 879–896.
105. Jensen, S.I., Lewis, R.J., Hoagland, K.D. Genetic variation in Fragilaria capucina clones along a latitudinal gradient[J]. J Phycol, 1995, 31(3):18.
106. Jesus, G.M., Silvia, G.A., Ana, I.A, Francisco, R.V. Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity[J]. Journal of Microbiological Methods, 1999, 36: 55~64.
107. Jiao, N. Z., Sieracki, M.E., Zhang, Y., Du, H.L. Aerobic Anoxygenic Phototrophic Bacteria And Their Roles In Marine Ecosystems[J]. Chin Sci. Bull., 2003a 48(11): 1064-1068.
108. Jiao.,N. Z., Liu, C., Koshikawa, H., Watanabe, M. Dynamics of dimethyl sulfide and dimethyl- sulfoniopropionate in the China Seas[J]. Act. Botan. Sin., 2003b, 45 (7): 774-786.
109. Jiao, N.Z., Wang, R. Ammonium uptake and regeneration fluxes of the microplankton commumities in Jiaozhou Bay[J]. Chin. J. Oceanol. Limnol., 1994, 12(2): 165-174. (A)
110. Jiao, N.Z., Yang, Y. 2002a, Ecological Studies on Prochlorococcus in the China Seas[J], Chin. Sci. Bull., 47 (15): 1243-1250.
111. Jiao, N.Z., Yang, Y.H., Harada, S., Koshigawa, H., Watanabe, M. Responses of Picoplankton to Nutrient Perturbation in the South China Sea With Special Reference to the Coast-ward Distribution of Prochlorococcus[J]. Act. Botan. Sin., 2002b, 44(6): 731-739.
112. Jiao, N.Z., Yang, Y.H., Hong, N., Ma, Y., Koshikawa, H., Watanabe, M. Dyanmics of autotrophic picoplankton and heterotrophic bacteria in the East China Sea[J]. Continental Shelf Research, 2005, 25(7): 856-867.
113. Jiao, N.Z., Yang, Y.H., Koshikawa, H., Watanabe, M. Influence of hydrographic conditions onpicoplankton distribution in the East China Sea, a marginal sea of the Northwest Pacific. Aquatic Microbial Ecology, 2002, 30(1):37-48.
114. Jiao, N.Z., Zhang, Y., Chen, Y. Time series observation based Infrared epifluorescence microscopic approach for accurate enumeration of bacteriochlorophyll containing microbes in Marine Environments[J]. Journal of Microbiological Methods, 2005, 63(10): 1-11.
115. Jochem F.J., Pollehne F., Zeitzschel B. Productivity regime and phytoplankton size structure in the Arabian Sea. Deep-Sea Research Ⅱ , 1993, 40(3): 711~735.
116. Johnsen G , Samset O , Granskog L, In vivo absorption characteristics in 10 classes of bloom-forming phytoplankton: taxonomic characteristics and responses to photoadaptation by means of discriminant and HPLC analysis[J]. Mar. Ecol. Prog. Ser., 1994,105: 149–157.
117. Kim M., W. Philpot . Ocean pigment algorithm based on the phytoplankton particle optics[M]. Ocean Optics XV, Monaco, 2000
118. Knight Jones, EW Aspects of the setting behaviour of larvae Ostrea edulis on Essex oyster beds[J]. Rapp. Cons. Explor. Mer. 1951,128 (II).
119. Kooistra W.H.C.F, Gersonade R, Sims P. Oraginand evolution of the diatoms on 18s rRNA sequences[J]. J Phycol , 1995, 31(3):18.
120. Landry, M.R. and D.L. Kirchman. Microbial community structure and variability in the the tropical Pacific[J]. Deep-Sea Res. II. 2002,49:2669-2693.
121. LaRoche, J., Van Der Staay, G.W.M., Partensky, F., Ducret, A., Aebersold, R., Li, R., Golden, S.S., Hiller, R.G., Wrench, P.M., Larkum, A.D., Green, B. Independent evolution of the Prochlorophyte and green plant chlorophyll a/b light-harvesting proteins[J]. Proc. Nat. Acad. Sci. U.S.A., 1996, 93: 15444~15248.
122. Latasa M., Bidigare R.R., A comparison of phytoplankton populations of the Arabian Sea during the Spring Intermonsoon and Southwest Monsoon of 1995 as described by HPLC-analysed pigments[J], Deep-Sea Res., 1998, 45: 2133–2170.
123. Leeuwe M.A., Stefels J., Effect of iron and light stress on the biochemical composition of the Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition[J], J. Appl. Phycol., 1998, 34: 496–503.
124. Lenaers G. Dinoflagellates in evolution: A molecular phylogenetic analysis of large subunit ribosomal RNA[J]. J. Mol. Evol. 1989, 29: 40-51.
125. Lenaers G. A molecular phylogeny of dinoflagellate protists (Pyrrophyta) inferred from the sequence of 24s rRNA divergent domains D1 and D8[J]. J. Mol. Evol. 1991,32: 53-63.
126. Lerman A, Control on river water composition and the mass balance of river systems[M]. In: Martin J M, Burton J D, Eisma D (eds) , River Inputs to Ocean Systems, Proceedings of a SCOR/ACMRR/ECOR /IAHS/UNESCO /CMG/IABO/IAPSO Review and Workshop, March 26~30, 1979, Rome, Italy, 1981, pp1-4.
127. Lerman, S. and Manski, C. On the Use of Simulated Frequencies to Approximate Choice Probabilities[M], of: Manski, C., and McFadden, D. (eds), Structural Analysis of Discrete Data with Econometric Applications. MIT Press. 1981.
128. Letelier, R.M., Bidigare, R.R., Hebel, D.V. Temporal variability of phytoplankton community structure at the U.S.-JGOFS time-series Station ALOHA based on HPLC pigment analysis[J]. Limn.and Ocean., 1993, 38: 1420-1437.
129. Li, W.K.W. Autotrophic picoplankton in the tropical ocean[J]. Science, 1983, 219: 292-295.
130. Liaaen-Jensen, S., Andrewes, A.G. Analysis of carotenoids and related polyene pigments. Methods in microbiology[M]. G. Gottschalk (ed.)., Acad. Press, New York–London, 1985, 235–283.
131. Lindell, D., and A.F. Post Ultraphytoplankton succession is triggered by deep winter mixing in the Gulf of Aqaba (Eilat), Red Sea[J]. Limnol. Oceanogr. 1995,40(6): 1130-1141
132. Llewellyn C.A. and R.F.C. Mantoura, Pigment biomarkers and particulate carbon in the upper water column compared to the ocean interior of the northeast Atlantic[J]. Deep-Sea Res.I, 1996,43:1165-1184.
133. Lomas MW, PM Glibert, DA Clougherty, DR Huber, Elevated organic nutrient ratios associated with brown tide algal blooms of Aureococcus anophagefferens (Pelagophyceae), Journal of Plankton Research,2001,23 (12) :1339-1344
134. Longhurst and Harrison, The biological pump: Profiles of plankton production and consumption in the upper ocean, Progress in Oceanography 1989, 22: 47-123.
135. Lopez-Garcia, P., F. Rodriguez-Valera, C. Pedros-Alio, and D. Moreira. Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. 2001, Nature 409:603-607.
136. Lu C. K., Chou H.N., Lee C.K., Lee T.N., Lefranc M, Thénot A, Lepère C, Debroas D. Genetic Diversity of Small Eukaryotes in Lakes Differing by Their Trophic Status[J]. Appl. Environ. Microbiol. 2005, 71:5935–5942.
137. Lu, Y Qi, J Goebel, J Zou, Y Gao. Redescription of Prorocentrum donghaiense Lu and comparison with relevant Prorocentrum species[J]. Appl. Ecol. Bull. 2003 14(7):1060
138. Ma Y, Jiao N Z, Molecular ecology of marine Synechococcus[J]. Prog. Natural Sci., 2004,14(8): 649-655
139. Ma, Y., Jiao, N.Z., Zeng, Y.H. Natural Community structure of cyanobacteria in the South China Sea as revealed by rpoC1 gene sequence analysis[J]. Lett. Appl. Microbiol., 2004, 39: 353-358.
140. Mackey, D,J., Higgins, H.W., Mackey, M.D. Algal class abundances in the western equatorial Pacific: estimation from HPLC measurements of chloroplast pigments using CHEMTAX[J]. Deep-Sea Research I, 1998, 45:1441–1468.
141. Mackey, D.J., Blanchot, J., Higgins, H.W., Neveux, J. Phytoplankton abundances and community structure in the equatorial Pacifi[J]c. Deep-Sea Res. II, 2002, 49: 2561-2582.
142. Mackey, D.J., Parslow, J.S., Griffiths, F.B. Phytoplankton productivity and the carbon cycle in the western equatorial Pacific under ENSO and non-ENSO conditions[J]. Deep-Sea Res. II, 1997, 44: 1951-1978.
143. Mackey, M.D., Mackey, D.J., Higgins, H.W. CHEMTAX a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton[J]. Mar. Ecol. Prog. Ser, 1996, 144: 265–283.
144. Majchrowski R., Ostrowska M., Influence of photo- and chromatic acclimation on pigment composition in the sea[J], Oceanologia, 2000, 42 (2): 156-165.
145. Marie D, Brussaard CPD, Partensky F, Vaulot D. Flow cytometric analysis of phytoplankton, bacteria and viruses[M]. Current Protocols in Cytometry, Vol. 11.11 (Robinson JP, ed), pp. 1–15. John Wiley & Sons, New York. 1999.
146. Marie D., Fei Zhu, Vanessa Balagué, Joséphine Ras, & Daniel Vaulot. Eukaryotic picoplankton communities of the Mediterranean Sea in summer assessed by molecular approaches (DGGE, TTGE, QPCR)[J]. FEMS Microbiology Ecology , 2006 , 55(3): 403-415.
147. Marie, D., Simon, N. & Vaulot, D. Phytoplankton cell counting by flow cytometry[M], p. 253-267. In Andersen, R. A. [ed.], Algal Culturing Techniques. Academic Press. 2005.
148. Mark Trice T., M. G. Patricia, L. Chris, , HPLC pigment records provide evidence of past blooms of Aureococcus anophagefferens in the Coastal Bays of Maryland and Virginia[J], USA.Harmful Algae, 2004, 3, 295-304 .
149. Masato H., K. Furuya , H. Hattori, Spatial heterogeneity in distributions of chlorophyll a derivatives in the subarctic north pacific during summer[J]. J. Oceanogr., 2001, 57, 323-331.
150. Massana, R. and K. Jürgens, Composition and population dynamics of planktonic bacteria and bacterivorous flagellates in seawater chemostat cultures[J]. Aquatic Microbial Ecology, 2003, 32: 11-22.
151. Massana, R., BalaguéV, Guillou L, Pedrós-AlióC. Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches[J]. Microbiology Ecology, 2004, 50: 231–243.
152. Massana, R., Guillou, L., D?′ez, B. and Pedros-Alio , C. Unveiling the organisms behind novel eukaryotic ribosomal DNA sequences from the ocean[J]. Appl. Environ. Microbiol. 2002,68: 4554–4558.
153. Medliin E.K., Elwood H.J., Stickel S., et al. Morphological and genetic variation withinn the diatom Skeletonema costatum: evidence for a new species Skeletonema pseudocostatum[J]. J Phycol, 1991, 27:514-524.
154. Meyr-Harms, B. & F. Pollenhe, Alloxanthin in Dinophysis norvegica (Dinophysiales, Dinophyceae ) from the Baltic Sea[J]. J Phycol, 1998, 34: 280-285.
155. Michot B, Hassouna N, Bachellerie JP. Secondary structure of mouse 28S rRNA and general model for the folding of the large rRNA in eukaryotes[J]. Nucleic Acids Res. 1984 May 25;12(10):4259–4279.
156. Mikel, L., Robert, R.B. A compareison of phytoplankton populations of the Arabian Sea during the Spring Intermonsoon and Southwest Monsoon of 1995 as described by HPLC-analyzed pigments[J]. Deep-Sea Res Ⅱ, 1998, 45:2133-2170.
157. Moestrup,. and Thomsen,H.A. (1980) Preparation of shadowcast whole mounts[M]. In Gantt,E. (ed.), Handbook of Phycological Methods: Developmental and Cytological Methods. University Press, Cambridge, Vol. 8, pp. 386–390.
158. Moon-van der Staay, S.Y., De Wachter, R., Vaulot, D. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected-eukaryotic diversity[J]. Nature, 2001, 409: 607–610.
159. Moore LR and SW Chisholm. Photophysiology of the marine cyanobacterium Prochlorococcus: Ecotypic differences among cultured isolates[J]. Limnol. Oceanogr.,1999,44(3):628-638
160. Moore LR, G Rocap, and SW Chisholm. Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes[J]. Nature. 1998,393:464-467
161. Moore, L. R., R. Goericke, and S. W. Chisholm, Comparative physiology of Synechococcus and Prochlorococcus: Influence of light and temperature on growth, pigments, fluorescence and absorptive properties[J], Mar. Ecol. Prog. Ser., 1995, 116: 259-275.
162. Morden CW, Delwiche CF , Kuhsel M , Palmer JD , Gene phylogenies and the endosymbiotic origin of plastids. Biosystems[J] 1992,28 (1 - 3) : 75 - 90.
163. Moreira,D. and Lo′pez-Garc?′a,P. The molecular ecology of microbial eukaryotes unveils a hidden world. Trends in Microbiology [J], 2002, 10:31–38.
164. Morton B., Wu S. S. The hydrology of the coastal waters of Hong Kong[J]. Environmental Research,1975 10, 19~347.
165. Mukai, T., Effects of micro-scale in situ environmental gradients concerning water qualities on the structure of the phytoplankton community in a coastal embayment. Estuarine[J], Coastal Shelf Sci. , 1987, 25: 447-458.
166. Murphy, L.S., Haugen, E.M. The distribution and abundance of phototrophic ultraplankton in the North Atlantic[J]. Limnol. Oceanogr., 1985, 30: 47–58.
167. Murray, A.E., Hollibaugh, J.T. and Orrego, C., Phylogenetic composition of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments[J]. Appl Environ Microbiol., 1996,62:2676~2680.
168. Muyzer, G. DGGE/TGGE, a method for identifying genes from natural communities[J]. Curr. Opin. Microbiol., 1999, 2: 317–322.
169. Muyzer, G., De Waal, E.C., Uitterlinden, A.G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Appl. Environ. Microbiol., 1993, 59: 695–700.
170. Muyzer, G., T. Brinkhoff, U. Nübel, C. Santegoeds, H. Sch?fer, and C. Wawer. Denaturing gradient gel electrophoresis (DGGE) in Microbial Ecology[M]. In G. A. Kowalchuk, F. J. de Bruijn, I. M. Head, A. D. Akkermans, and J. D. van Elsas (ed.), Molecular Microbial Ecology manual, 2nd ed. Kluwer Academic Publishing, London, England, 2004.
171. Nellan, B.A. Identification and phylogenetic analysis of toxigenic Cyanobacteria by multiplex randomly amplified polymorphic DNA PCR[J], Appl Environ Microbiol, 1995, 61: 2286~2291.
172. Neufeld Josh D. and William W. Mohn , Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis[J]. Appl Environ Microbiol. 2005 ,71(8): 4893–4896
173. Not F, Simon N, Biegala I, Vaulot D. Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition[J]. Aquatic Microbial Ecology, 2002, 28: 157-166.
174. Not, F., Latasa, M., Marie, D., Cariou, T., Vaulot, D. & Simon, N, A single species Micromonas pusilla (Prasinophyceae) dominates the eukaryotic picoplankton in the western English Channel[J]. Appl Environ Microbiol, 2004, 70: 4064-4072.
175. Nubel U, Engelen B., Felske A., , Sequence heterogeneities of genes encoding 16S rRNAs in paenibacillus polymyxa detected by temperature gradient gel electrophoresis[J]. Journal of Bacteriology, 1996, 178:5636~5643.
176. Obayashi Y., E. Tanou , Growth and mortality rates of phytoplankton in the northwestern North Pacific estimated by the dilution method and HPLC pigment analysis[J]. J. Exp. Mar. Bio. Eco. 2002, 280 , 33- 52.
177. Odate, T., M. Yanda, H. Mizuta, and Y. Maita. Phytoplankton carbon biomass estimated from thesize fractionated chlorophyll a concentration and cell density in the northern coastal waters from spring bloom tosummer[J]. Bulletin of Plankton Society of Japan, 1993,39: 127-144
178. Olsen, GJ, Lane, DL, Giovannoni, SJ, & Pace, NR . Microbial Ecology and evolution: A ribosomal RNA approach. Ann. Rev. Microbiol[J]., 1986, 40: 337-365.
179. Olson, R.J., Chisholm, S.W., Zettler, E.R. Spatial and temporal distributions of prochlorophyte picoplankton in the North Atlantic Ocean[J]. Deep-Sea Res, 1990, 37: 1033-1051.
180. Pace B, Matthews EA, Johnson KD, Cantor CR, Pace NR. Conserved 5S rRNA complement to tRNA is not required for protein synthesis[J]. Proc Natl Acad Sci U S A. 1982;79(1):36–40.
181. Partensky F, Blanchot J and Vaulot D. Differential distribution of Prochlorococcus and Synechococcus in oceanic waters: a review[M]. In: Charpy L, Larkum A.W.D. (eds), Marine cyanobacteria. Bulletin de l'Institut Océanographique de Monaco No sp. 1999b.19: 457-475
182. Partensky F, Hess WR and Vaulot D. Prochlorococcus, a marine photosynthetic prokaryote of global significance[J]. Microbiology and Molecular Biology Reviews 1999a. 63: 106-127
183. Partensky F., Guillou L. Recent advances in the use of molecular techniques to assess the genetic diversity of marine photosynthetic microorganisms[J]. Genetic diversity of marine picophytoplankton. 1997,47(4):367-374.
184. Perasso, R., Baroin, A., Qu, L. H., Bachellerie, J. P. & Adoutte, A. Origin of the algae. Nature 1989,339:142–4.
185. Pinckney, J.L., Paerl, H.W., Harrington, M.B. Annual cycles of phytoplankton community-structure and bloom dynamics in the Neuse River Estuary, North Carolina [J]. Marine Biology, 1998, 131: 371–381.
186. Pitta, P., Karakassis, I. Size distribution in ultraphytoplankton: a comparative analysis[J]. Environ. Monit. Assess. 2005,102: 85-101.
187. Platl.T., D.V.Subba.Rao and B.Erwin, Photosynthesis of picoplankton in the oligotrophic ocean [J]. Nature, 1983, 301:702~704.
188. Platt, T. , W. G. Harrison, Biogenic fluxes of carbon and oxygen in the ocean [J]. Nature, 1985,318: 55-58.
189. Poryvkina, L, Babichenko, S, Kaitala, S, Spectral fluorescence signatures in characterization of phytoplankton community composition [J]. Journal of Plankton Research,1994,16(10): 1315-1327.
190. Potter D, T C Lajeunesse, G W Saunders, R A Andersen. Convergent evolution masks extensive biodiversity among marine coccoid picoplankton [J]. Biodivers. Conserv. 1997, 6:99–107.
191. Qu HL, Michot B, Bachellerie JP. Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA [J]. Nucleic Acids Res. 1983 Sep 10;11(17):5903–5920.
192. Qu LH, Nicoloso M, Bachellerie JP. Phylogenetic calibration of the 5' terminal domain of large rRNA achieved by determining twenty eucaryotic sequences [J]. J Mol Evol.1988, 28(1-2):113–124.
193. Rappé, M. S., M. T. Suzuki, K. L. Vergin, and S. J. Giovannoni. Phylogenetic diversity of ultraplankton plastid small-subunit rRNA genes recovered in environmental nucleic acid samples from the Pacific and Atlantic coasts of the United States [J]. Appl. Environ. Microbiol. 1998, 64:294-303.
194. Riegman, R, Rowe, A, Nutritional status and pigment composition of phytoplankton during springand summer Phaeocystis blooms in Dutch coastal waters (Marsdiep area) [J]. Neth. J. Sea Res, 1994, 32:13-21
195. Riegman, R., M. Boer, L.D. Domis, Growth of harmful marine algae in multispecies cultures [J]. J. Plankt. Res. 1996, 18:1851-1866.
196. Rocap, G., D. L. Distel, J. B. Waterbury, And S. W. Chisholm. Resolution Of Prochlorococcus and Synechococcus Ecotypes by Using 16S-23S Ribosomal DNA Internal Transcribed Spacer Sequences [J]. Appl. Environ. Microbiol. 2002, 68: 1180-1191.
197. Rodríguez, F., Derelle, E., Guillou, L., Le Gall, F., Vaulot, D. & Moreau, H. Ecotype diversity in the marine picoeukaryote Ostreococcus (Chlorophyta, Prasinophyceae) [J]. Environmental Microbiology, 2005, 7:853-859
198. Romari, K., Vaulot, D. Composition and temporal variability of picoeukaryote communities at a coastal site of the English Channel [J], Limnol. Oceanogr. 2004, 49:784-798
199. Sadoudi, N., Babin, M., Allali, K. Spatial variations of photosynthetic parameters in equatorial Pacific: forcing by vertical mixing and light penetration. Ocean Optics 13, Proc. SPIE, 1996, 2963: 880–885.
200. Santegoeds, C.M., Nold, S.C., Ward, D.M. Denaturing gradient gel electrophoresis used to monitor the enrichment culture of aerobic chemoorganotrophic bacteria from a hot spring cyanobacterial mat [J]. Appl Environ Microbiol, 1996, 62: 3922~3928.
201. Sautour, B.; F. Artigas; A. Herbland & P. Laborde. Zooplankton grazing impact in the plume of dilution of the Gironde estuary (France) prior to the spring bloom [J]. Journal of Plankton Research, Oxford, 1996, 18: 835-853.
202. Scanlan, D.J., Hess, W.R., Partensky, F., Scanlan, J. and Vaulot, D. High degree of genetic variation in Prochlorococcus (Prochlorophyta) revealed by RFLP analysis. European [J] . J Phycol. 1996, 31: 1-9.
203. Schluter, L., Havskum, H. Phytoplankton pigments in relation to carbon content in plankton communities [J]. Mar. Ecol. Prog. Ser., 1997, 155: 55–65.
204. Schluter, L., M?hlenberg, F., Havskum, H., Larsen, S. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas, testing the influence of light and nutrients on pigment/chlorophyll a ratios [J]. Mar. Eco. Pro. Ser., 2000,, 192: 49-63.
205. Shao, P., Chen, Y.Q., Zhou, H., Qu, L.H., Ma, Y., Li, H.Y., Jiao, N.Z. Phylogenetic diversity of Archaea in prawn farm sediment [J]. Marine Biology, 2004, 146 (1): 133-142.
206. Sherr, E.B., and B.F. Sherr. Bacterivory and herbivory: Key roles of phagotrophic protists in pelagic food webs [J]. Microbial Ecology , 1994, 28: 223-235.
207. Sieburth, J.Mc.N., Smetacek, V., Lenz, J. Pelagic ecosystem structure, Heterotrophic compartments of the plankton and their relationship to plankton size fraction [J]. Limnol. Oceanogr., 1978, 23: 1256-1263.
208. Sigler, W.V., Miniaci, C., Zeyer, J. Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure [J]. Journal of Microbiological Methods, 2004, 57: 17–22.
209. Sinha R.P., M. Klisch , A. Groniger, D.-P. Hader, Ultraviloet-absorbing/screening substances in cyanobacteria, phytoplankton and macroalgae [J].J. Photochem. Photobiol, B,Biol. 1998, 47:83-94.
210. Smalla, K., Wieland, G., Buchner, A. Bulk and rhizosphere soil bacterial communities studied bydenaturing gradient gel electro-phoresis:plang dependent enrichment and seasonal shifts revealed [J]. Appl. Environ. Microbiol., 2001, 67: 4742–4751.
211. Stahl, DA. Analysis of hydrothermal vent-associated symbiont by ribosomal RNA sequences [J]. Science. 1984, 224: 409-411.
212. Stoeck,T., Taylor,G.T. and Epstein,S.S. Novel eukaryotes from the permanently anoxic Cariaco Basin (Caribbean Sea) [J]. Appl. Environ. Microbiol. 2003, 69:5656–5663.
213. Stoltes, W. ,G.W. Kraay, A.M. Noordeloos , Genetic and physiological variation in pigment composition of Emiliania Huxleyi (Prymnesiophyceae) and the potential use of its pigment ratios as a quantitative physiological marker [J]. J Phycol, 2000,36:529-539.
214. Ston J, Alicja Kosakowska Qualitative and quantitative analysis of Baltic phytoplankton pigments [J]. Oceanologia 2000, 42 (4): 449-471.
215. Stoń, J. and A. Kosakowska, Qualitative and quantitative analysis of Baltic phytoplankton Pigments [J].,. Oceanologia, 2000, 42 ,4, 449-471.
216. Suzuki M T, M S Rappé, and S J Giovannoni. Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity [J]. Appl. Environ. Microbiol. 1998, 64:4522–4529.
217. Suzuki, K. Minami, C., Liu, H., Saino, T., Temporal and spatial patterns of chemotaxonomic algal pigments in the subarctic Pacific and the Bering Sea during summer 1999 [J]. Deep-Sea Res. II , 2002., 49:5685-5704
218. Suzuki, K., Handa, N. Estimation of phytoplankton succession in a fertilized mesocosm during summer using high-performance liquid chromatographic analysis of pigments [J]. J. Exp. Mar. Bio. Eco.,1997, 214: 1-17.
219. Takahashi M. and P.K.Bienfang. 1983. Size structure of phytoplankton biomass and photosynthesis in subtropical Hawaiian waters [J]. Mar. Biol., 76(2): 203~211.
220. Tang D.L. , D. R. Kester, I-H. Ni, Y.Z. Qi, H. Kawamura., In situ and satellite observations of a harmful algal bloom and water condition at the Pearl River estuary in late autumn 1998 [J]. Harmful Algae. 2003, 2: 89-99.
221. Tengs, T., Dahlberg, O.J. Phylogenetic analyses indicate that the 19’ hexanoyloxy- fucoxanthin- containing dinoflagellates have tertiary plastids of haptophyte origin. Mol. Biol. Evol., 2000, 17(5): 718-729.
222. Thomsen,H.A. A survey of the smallest eukaryotic organisms of the marine phytoplankton[M]. In Platt,T. and Li,W.K.W. (eds), Photosynthetic Picoplankton. Can. Bull. Fish. Aquat. Sci.,1998, 214:121–158.
223. Throndsen,J. Estimating cell numbers[M]. In Hallegraeff,G.M., Anderson,D.M. and Cembella,A.D. (eds), Manual on Harmful Marine Microalgae. IOC Manuals and Guides, 33. UNESCO, Paris, 1995.pp. 63–80.
224. Throndsen,J. and Zingone,A. Micronomads of the Mediterranean Sea[J]. G. Bot. It., 1994.128: 1031–1044.
225. Tolmazin D. Elements of Dynamic Oceanography[M]. Boston, Allen & Unwin. 1985.
226. Tyson GW., Community Structure and Metabolism Through Reconstruction of Microbial Genomes From the Environment [J], Nature, 2004, 428: 37.
227. Urbach, E., Sallie, W.C. Genetic diversity in Prochlorococcus populations flow cytometrically sorted from Sargasso Sea and Gulf Stream [J]. Limnol. Oceanogr. 1998, 43(7): 1615~1630.
228. Vaitomaa, J., and others Quantitative real-time PCR for determination of microcystin synthetase E copy numbers for Microcystis and Anabaena in lakes [J]. Appl. Environ. Microbiol. 2003.69:7289-7297.
229. Van de Peer Y, S Chapelle and R De Wachter, conserved regions of rRNA molecules [J]. Nucleic Acids Res., 1996, 24(17): 3381-3391
230. Van Leeuwe, M A, Stefels, J, Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition [J]. J Phycol, 1998,34: 496–503.
231. Vaulot D. Phytoplankton. Encyclopedia of Life Sciences[M], http://www.els.net, Nature Publishing Group. London 2001.
232. Vaulot, D. and Partensky F., Cell cycle distribution of prochlorophytes in the north western Mediterranean Sea [J]. Deep Sea Res. 1992. 39(5):727-742
233. Veldhuis M. J. W., and Kraay G.W., Vertial distribution and pigment composition of a picoplanktonic prochlorophyte in the subtrophical N Atlantic: a combined study of HPLC-analysis of pigments and flow cytometry [J]. Mar. Ecol. Prog. Ser 1990,68: 121~127
234. Venter,J.C., Remington, K. ,Environmental genome shotgun sequencing of the Sargasso Sea [J]. Science. 2004 ,304(5667):58-60.
235. Verity, P.G., Paffenhofer, G.A., Wallace, D., Sherr, E., Sherr, B., Composition and biomass of plankton in spring on the Cape Hatteras shelf, with implications for carbon flux [J]. Continental Shelf Research, 1996.16, 1087–1116.
236. Verity, P.G., Redalje, D.G., Lohrenz, S.E., Flagg, C., Hristov, R., Coupling between primary production and pelagic consumption in temperate ocean margin pelagic ecosystems [J]. Deep-Sea Research II, 2002. 49 (20): 4553–4569.
237. Waterbury J B. Photosynthetic picoplankton [J]. Can. Bull. F ish. A quat. Sci. , 1986, 214: 71-120
238. Ward, D.M., Ferris, M.J., Nold, S.C., Basteson, M.M.A. natural view of microbial biodiversity within hot spring cyanobacterial communities [J]. Appl. Environ. Microbiol., 1998, 62: 1353–1370.
239. Watts, Betty H. The national workshop on Aboriginal education: Priorities for action and research. Brisbane[M], Australia: University of Queensland Press. 1971.
240. Weber L.H. and S.Z.El-Sayed. 1987. Contributions of the net-.nano-,and picoplankton to the phytoplankton standing crop and primary productivity in the Southern Ocean [J]. J. Plankton Res., 9: 973~994.
241. William J.G.K.,Kubelik AR,Livak K.J. DNA polymorphisms amplified by arbitary primers and ureful as genetic markers [J] Nucleic Acids Res., 1990.18(22): 6531-6535.
242. Wong C. K. ,C. K. Wong, HPLC pigment analysis of marine phytoplankton during a red tide occurrence in Tolo Harbour, Hong Kong Chemosphere .2003.52 ,1633-1640.
243. Worden A.Z., Nolan J.K. , Palenik B. Assessing the dynamics and ecology of marine pico- phytoplankton : The importance of the eukaryotic component [J]. Limnol. Oceanogr. 2004, 49:168– 179.
244. Wright S.W., Jeffrey S.W., Fucoxanthin pigment markers of marine phytoplankton analysed byHPLC and HPTLC [J], Mar. Ecol. Prog. Ser., 1987, 38:259–266.
245. Wright S.W., Shearer J.D., Rapid extraction and high-performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton [J], J. Chromatogr., 1984,294: 281–295.
246. Wright, S W , van den Enden, R L , Stratification/mixing regimes control phytoplankton populations off East Antarctica: evidence from CHEMTAX analysis of HPLC pigment profiles (BROKE survey, Jan–Mar 1996). Deep-Sea Research II,2000, 47: 2363–2400.
247. Wright, S.W., S.W. Jeffrey, F.C. Mantoura, C.A. Llewellyn, T. Bj?rnland, D. Repeta, and N. Welschmeyer . Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton [J]. Mar. Ecol. Prog. Ser. 1991. 77:183-196.
248. Wright, S.W., Thomas, D.P., Marchant, H.J. Analysis of phytoplankton of the Australian sector of the Southern Ocean: comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the ‘CHEMTAX’ matrix factorisation program [J]. Mar. Ecol. Prog. Ser, 1996, 144: 285–298.
249. Wulff A., S.A. Wangberg, Spatial and vertical distribution of phytoplankton pigments in the eastern Atlantic sector of the Southern Ocean [J]., Deep-Sea Res. II 2004, 51:2701-2713.
250. Wyrtki K., Physical Oceanography of the Southeast Asia Waters[M]. NAGA Report,2,1-195, 1961.
251. Yacobi Y. Z., Pollingher U., Gonen Y., Gerhardt V., Sukenik A., HPLC analysis of phytoplankton pigments from Lake Kinneret with special reference to the bloom-forming dinoflagellate Peridinium gatunese (Dinophyceae) and chlorophyll degradation products [J], J. Plankt. Res., 1996, 18 (10): 1781–1796.
252. Yang, Y.H., Jiao, N.Z. Dynamics of picoplankton in the Nansha Islands area of the South China Sea [J] Act. Oceanol. Sin., 2004, 23(3): 493-504.
253. Ypma, Jan Erik & Jahn Throndsen Seasonal dynamics of bacteria, autotrophic picoplankton and small nanoplankton in the Inner Oslofjord and the Skagerrak in 1993 [J]. Sarsia. 1996. 81:57-66.
254. Yuan, J.M, Chen, S., Zhou, P.H., Chen, Y., Qu, L. Genetic diversity of small eukaryotes from the coastal waters of Nansha Islands in China. FEMS Microbiology. Letters [J]. 2004, 240(2):163–170.
255. Zapata, M, Garrido, J L, Occurrence of phytylated chlorophyll c in Isochrysis galbana and Isochrysis sp. (Clone T-ISO) (Prymnesiophyceae) [J]. J Phycol, 1997,33: 209–214.
256. Zapata M, Rodríguez F, Garrido JL. Separation of. chlorophylls and carotenoids from marine phytoplankton:. a new HPLC method using a reversed-phase C8 column. and pyridine-containing mobile phases [J]. Mar Ecol Prog Ser. 2000.195:29–45
257. Zeidner G, Preston CM, Delong EF, Massana R, Post AF, Scanlan DJ, Béjà O, Molecular diversity among marine picophytoplankton as revealed by psbA analyses [J]. Environ Microbiol, 2003,5:212–216
258. Zeng,Y.H., Jiao,N.Z., Cai,H.Y., Chen,X.H., Wei, C.L. Phylogenetic diversity of ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea [J]. Act. Oceanol. Sin., 2004, 23(4): 673-685.
259. Zettler, L. A. A., F. Gomez, E. Zettler, B. G. Keenan, R. Amils, and M. L. Sogin. Eukaryotic diversity in Spain' s River of Fire [J]. Nature .2002. 417:137.
260. Zhu, F., Not, F., Massana, R., Marie, D. & Vaulot, D. Mapping of the picoeucaryotes in marine systems with quantitative PCR of the 18S rRNA gene [J]. FEMS Microbiology Ecology. 2005. 52:79-92
261. 蔡清海,吴善,阮秀凤.厦门西海域盐度的变化[J].热带海洋学报,1993,(3):104~107
262. 蔡昱明,宁修仁,刘子琳, 珠江口初级生产力和新生产力研究[J].海洋学报 , 2002.24(3):101-111.
263. 曹振锐,黄邦钦,刘媛,洪华生,谢廷贵.厦门海域分粒级叶绿素 a 含量的分布特征[J].台湾海峡,2005,(04): 493-498.
264. 陈冠惠、黄荣祥、梁红星等,海洋水文气象 V. 气象和气候,台湾海峡中北部海洋调查研究报告[M],科学出版社,北京,1998
265. 陈怀清,钱树本,1992. 青岛近海微型超微型浮游藻的研究[J]. 海洋学报, 14(3): 105-113.
266. 陈纪新, 黄邦钦, 贾锡伟, 洪华生, 谢廷贵. 利用光合色素研究厦门海域微微型浮游植物群落结构[J]. 海洋环境科学. 2003, 22(3):16–21.
267. 陈金泉,傅子琅,李法西.关于闽南-台湾浅滩渔场上升流的研究[J].台湾海峡,1982,1(2):5-13.
268. 陈敏艺,袁洁,陈月琴,屈良鹄.海洋超微型浮游植物遗传多样性的分子系统学研究进展[J].自然科学进展,2005,(9):1032-1040
269. 陈其焕,陈兴群,张明.厦门港赤潮发生区叶绿素a的分布变化特征.厦门港赤潮调查研究论文集[M].北京:海洋出版社,1993,38-45.
270. 陈瑞祥,林景宏,林茂,戴燕玉.厦门西港浮游动物生态研究[J].台湾海峡,1998,(3): 294-298
271. 陈兴群,陈其焕,张明.厦门东侧水道海域的初级生产力[J].台湾海峡,2002,(2): 217-221
272. 陈兴群,陈其焕,庄亮钟,1989.南海中部叶绿素a分布和光合作用及其与环境因子的关系[J].海洋学报,11(3):349-355.
273. 陈映泰,罗章仁,1991.珠江口现代沉积速率及其反映的沉积特征[J]. 热带海洋,10(2):57-64.
274. 陈于望. 厦门港海域营养状况的分析 .海洋环境科学 [J],1987,(3):14~19.
275. 陈月琴等,南海赤潮有毒甲藻链状-塔玛亚历山大藻的分子鉴定[J],海洋学报,1999, 21(3):106-112
276. 陈宗团,1998. 珠江口陆海相互作用过程中稀土元素生物地球化学[D].博士学位论文,厦门大学,1-183.
277. 程兆第,高亚辉,刘师成.福建沿岸微型硅藻[M].北京:海洋出版社,1993,1-91
278. 程兆第,刘师成.西沙群岛海洋硅藻的研究──Ⅲ.胸隔藻属Mastogloia Thwaites 1新种及其他属种在我国的新记录[J].热带海洋1997,(4):25-31.
279. 杜庆红.厦门同安湾浮游植物变化的初步研究[J] .海洋通报,1996,(6): 20-26
280. 方少华,吕小梅,张跃平.厦门浔江湾小型底栖生物数量分布及生态意义[J] .台湾海峡,2000,(4):474~479
281. 冯士筰,李凤岐,李少菁, 海洋科学导论,[M] pp. 434-501. 高等教育出版社, 北京, 1999.
282. 付子琅.台湾海峡的上升流[J].海洋科学,1984,(2): 52~53.
283. 高亚辉,厦门港微型浮游生物研究[D].理学博士学位论文. 厦门大学. 1990.
284. 高亚辉,程兆第,金德祥.厦门港微型浮游生物叶绿素的分布和作用[J].海洋与湖沼,1994,25(1):87-93.
285. 管秉贤,1997. 海南岛以东外海的暖涡[J].黄渤海海洋,15(4):1-7.
286. 郭沛涌,沈焕庭,张利华.淡水微型浮游植物的FCM研究[J].中国环境科学,2002,(2):101~104
287. 国家海洋局第三海洋研究所. 厦门港湾海洋环境综合调查(第四章海洋生物) [J]. 台湾海峡,1988 ,7 (1) :1-10
288. 韩博平,韩志国,付翔,藻类光合作用机理及模型[M].科学出版社,北京. 2002.
289. 韩舞鹰,马克美, 粤东沿岸上升流的研究[J], 海洋学报, 1988, 8(4): 16-20.
290. 韩舞鹰,马克美, 粤东沿岸上升流氧的各种变化过程[J], 热带海岸,1990,10(3): 65-70.
291. 韩鹰舞,南海海洋化学[M]. 298pp.科学出版社, 北京,1998.
292. 何学佳,厦门西海域浮游植物色素的研究[D] .硕士学位论文, 厦门大学, 2003.
293. 洪华生,戴民汉,黄邦钦等,厦门港浮游植物对磷酸盐吸收速率的研究[J],海洋与湖沼,1994,25(1):54-59;
294. 洪华生,黄邦钦.厦门西海域秋季浮游植物现存量光合作用率的分级研究[J].厦门大学学报 ,1994,33(增刊):12-16.
295. 洪华生,商少凌,张彩云,黄邦钦,胡建宇,黄加祺,卢振彬.台湾海峡生态系统对海洋环境年际变动的响应分析[J].海洋学报 ,2005,(2): 63-68
296. 洪华生等,闽南-台湾浅滩渔场上升流区生态系研究[J],科学出版社, 北京, 1991
297. 洪鹰,李立.台湾海峡南部及附近海域夏季的陆架-陆坡锋[J].台湾海峡,1999,(2):159-162
298. 胡建宇,洪华生,贺志刚等,1998年8月台湾海峡表层温、盐度分析[J],热带海洋,2000,19(4):15-22
299. 黄邦钦,洪华生,柯林,曹振锐,2005,珠江口分粒级浮游植物叶绿 a 和初级生产力研究[J], 海洋学报,27(6):180-185.
300. 黄邦钦,洪华生,王大志,林学举,张钒,刘媛.台湾海峡浮游植物生物量和初级生产力的粒级结构及碳流途径[J].台湾海峡,2002,21(1):23-30.
301. 黄邦钦,洪华生,薛雄志.厦门西海域水体中碱性磷酸酶活力分布及其影响因子分析[J] .海洋学报 ,2000,(1): 62-68.
302. 黄邦钦,黄世玉,翁妍,洪华生.溶解态磷在海洋微藻碱性磷酸酶活力变化中的调控作用[J] .海洋学报 ,1999,(1): 55-60.
303. 黄邦钦,林学举.厦门西侧海域超微型浮游植物的时空分布及其调控机制[J],台湾海峡,2000,19(3):329–336.
304. 黄邦钦,王海黎,洪华生,商少凌.厦门海域浮游植物和细菌对溶解有机磷的利用[J].厦门大学学报 ,1996,(04): 625-630.
305. 黄良民,1992.南海不同海区叶绿素a和海水荧光值的垂向变化[J].热带海洋,11(4):89-95.
306. 黄良民,1992.珠江口水域叶绿素a和类胡罗卜素的周年分布[J].海洋环境科学,11(2): 13-18.
307. 黄良民,陈清潮,黄创俭,朱嘉濠等, 1994, 珠江口内海叶绿素a分布与环境因素关系[J], 生态学报,14(增刊):22-27.
308. 黄良民,陈清潮,林永水,南海北部海区浮游生物生产力分布初探[J].热带海洋研究, 1997, 5: 44-53.
309. 黄良民,陈清潮,尹建强,温伟英等, 珠江口及其邻近海域环境动态与基础生物结构初探[J]. 海洋环境科学, 1997.16(3): 1-7.
310. 黄小平,黄良民, 珠江口海域无机氮和活性磷酸盐含量的时空变化特征[J]. 台湾海峡, 2002. 21: 416-421.
311. 焦念志,陈念红.原绿球藻──海洋生态学研究的新领域[J].海洋科学,1995,(4):9-12
312. 焦念志,杨燕辉.中国海原绿球藻研究[J].科学通报,2002,(7):485-491.
313. 金德祥.厦门港1954年浮游生物的定量调查[J].厦门大学学报, 1955,5:17-30.
314. 蓝先洪. 珠江口沉积物的地球化学研究.见:张 经.中国主要河口的主物地球化学研究— 化学物质的迁移与环境[M] 北京:海洋出版社. 1996.
315. 李超伦,栾凤鹤.东海春季真光层分级叶绿素a分布特点的初步研究[J].海洋科学,1998,(4):59-62.
316. 李立, 南海上层环流观测研究进展[J]. 台湾海峡. 2002, 21: 114-125
317. 李立,郭小钢,吴日升.台湾海峡南部的海洋锋[J].台湾海峡,2000,(2):147-152.
318. 李立,李达.台湾浅滩西侧水道夏季的水文特征与上升流[J].台湾海峡,1989,8(4):353-359.
319. 李文权,厦门海沧沿岸水域初级生产力及其与环境的关系[J],热带海洋,1999,(3): 51-57.
320. 李雅琴,程兆第,金德祥.厦门港浮游硅藻生态的研究[J].厦门大学学报 ,1990,29(3):358-360.
321. 林更铭,杨清浪,林金美.厦门岛周围海域浮游植物的种类组成及丰度[J].台湾海峡,1994,13(4):3583-3584.
322. 林更铭,杨清浪,林金美.厦门岛周围海域浮游植物与环境因子的关系[J].海洋通报,1993,12(6):40-453.
323. 林洪瑛,韩舞鹰,王汉奎,程赛伟.南沙群岛海域溶解氧垂直分布最大值的季节特征[J].海洋学报,2001,(5): 51-58.
324. 林金美.厦门附近海域浮游甲藻类的分布. 生态学报,1990,(2): 21-26.
325. 林学举,黄邦钦,洪华生,王大志,肖天.东、黄海典型海域叶绿素 a 含量的垂向变化与周日波动[J].海洋科学,2002,(11): 57-63.
326. 刘仁沿,傅云娜,贺广凯,孙育红,孙茜,尚龙生,赵冬至.HPLC 方法分析表层海水叶绿素 a 含量[J]. 海洋通报,2004,(4): 75-79.
327. 刘媛,黄邦钦,曹振锐,洪华生.厦门海域春夏季微型浮游动物对浮游植物的摄食压力初探 .海洋环境科学,2005,(1): 1-12.
328. 卢振彬,戴泉水,颜尤明.台湾海峡及其邻近海域渔业资源生产力和最大持续产量.中国水产科学,2002,(01): 28-32.
329. 陆斗定,齐雨藻, Jeanette Goebel, 邹景忠,高亚辉.东海原甲藻修订及与相关原甲藻的分类学比较.应用生态学报,2003,(7): 1060-1065.
330. 罗冬莲.同安湾浮游动物数量的平面分布和季节变动 .福建水产,2004,(2).:83-87.
331. 宁修仁,海洋微型和超微型浮游生物.东海海洋,1997,15(3):60~64.
332. 宁修仁、史君贤、刘子彬、蔡昱明. 1996. 南大洋蓝细菌和微微型光合真核生物的丰度与分布. 中国科学(C辑),26(2):164-171.
333. 宁修仁,C.库蒂.长江口及冲淡水区叶绿素 a、细菌、ATP、POC 及微生物呼吸作用速率之间的关系[J].海洋学报,1991,(6):831-837.
334. 宁修仁,刘子琳,史君贤.渤、黄、东海初级生产力和潜在渔业生产量的评估[J].海洋学报 ,1995,(3):72-84.
335. 宁修仁,刘子琳,蔡昱明.我国海洋初级生产力研究二十年[J].东海海洋,2000,(3): 13-20.
336. 宁修仁,刘子琳,蔡昱明.象山港潮滩底栖微型藻类现存量和初级生产力[J].海洋学报 ,1999,(3):82-87.
337. 彭兴跃,洪海征,黄明等.厦门港海水光合色素特征[J].台湾海峡,2002,21(1):79-84.
338. 齐雨藻,中国沿海赤潮[M], p179, 科学出版社, 北京, 2003.
339. 屈良鹄 ,陈 月琴 .简单并 不代 表原 始── 关于生 物早期演 化的 一种 新观点[J].中 山大 学学报 ,1999,(1):128-129.
340. 商少凌,洪华生.厦门西海域环境参数的分布特征[J].厦门大学学报 ,1994. 33(增刊):97—101.
341. 邵鹏,袁洁,陈月琴,自然水样微型藻类遗传多样性方法学研究[J],海洋科学,2002,26(4):1~4.
342. 王海黎,洪华生,徐立.反相液相色谱法分离、测定海洋浮游植物的叶绿素和类胡萝卜素[J].海洋科学,1999,4:6-9.
343. 王海黎,洪华生.近岸海域光合色素的生物标志物研究Ⅰ台湾海峡特征光合色素的分布及其对浮游植物类群结构的指示[J],海洋学报,2000,22(3):94-102.
344. 王和勇,陈敏,廖志华,孙敏,RFLP、RAPD、AFLP 分子标记及其在植物生物技术中的应用[J],生物学杂志,1999,16(4):24~25.
345. 王寿景,李立,李燕初,陈季良,吴日升.厦门浔江湾海洋水文特征[J] .台湾海峡,1998,6(2): 201-206
346. 王伟强,张远辉,黄自强,九龙江口-厦门港河口盐度锋面的特征[J],台湾海峡,2000,19(1): 82-88.
347. 吴日升,李立.南海上升流研究概述.台湾海峡,2003,(2): 262-266.
348. 厦门海岛资源综合调查领导小组办公室等,厦门市海岛资源综合调查研究报告[M],北京:海洋出版社,1996, pp107-111.
349. 肖晖,郭小钢,吴日升.台湾海峡水文特征研究概述[J].台湾海峡,2002,(1):126-138.
350. 肖晖,台湾海峡西部沿岸上升流的研究[J],台湾海峡,1988,7(2): 141-142.
351. 徐锡祯, 南海水平环流概述[J], 中国海洋湖沼学会水文气象学会会议论文集, 科学出版社, 1980. 137-145.
352. 阎俊岳,陈乾金,张秀芝,黄爱芬, 中国近海气候[M]. 北京:科学出版社.1993, pp.109— 280.
353. 颜天,周名江,邹景忠等,香港及珠江口海域有害赤潮发生机制初步探讨[J]. 生态学报, 2001. 21(10): 1634-1641.
354. 杨海军,刘秦玉,南海海洋环流研究综述. 地球科学进展[J], 1998.13(4): 364-368.
355. 杨纪明.世界海洋渔业资源[J].海洋科学,1979,(2):112-117.
356. 杨清良,林更铭,蔡秉及.厦门东侧海域浮游植物的种类组成与分布[J].台湾海峡,2000,(3):337-341.
357. 杨 清 良 , 林 更铭 , 林金 美 厦门文 昌鱼 自然 保护 区 周围 海域 浮游植物 的生 态 [J]. 台湾海峡,1993,12(3):205-2172.
358. 杨清良,林更铭.厦门市海岛资源综合调查研究报告(第十章第三节浮游生物).厦门市海岛资源综合调查研究报告[J].北京:海洋出版社,1996.112-118.
359. 于大江主编,近海资源保护与可持续利用[J],北京:海洋出版社 2001.
360. 袁洁,邵鹏,陈月琴,蔡创华,屈良鹄.南沙群岛微型与超微型真核藻类遗传多样性的初步研究[J].海洋科学,2003,(7): 43-47.
361. 袁明, 珠温柔, 刘吉升等. 几种植物材料中总RNA的提取[J]. 分子植物育种. 2005, 3(2):285–292.
362. 曾刚.福建近海沿岸水及其水文状况[J].海洋通报,1986,(3): 32-37.
363. 张利华,流式细胞术在海洋浮游植物研究中的应用[J],东海海洋,1998,16(1):59~63.
364. 张学成主编,海藻遗传学[M],中国农业出版社,2005.
365. 赵焕庭等,珠江口评价[M]. 北京:海洋出版社,1990.
366. 周玉琴,厦门西港海域水质污染状况分析[J]. 海洋环境科学,1998,(4): 59-63.
367. 朱根海等. 南海浮游植物种类组成和丰度分布的研究[J].海洋学报,2003,25(2):8-23.
368. 庄宏儒,吕小梅,蔡励勋,黄槐,林情员,蒋龙成,沈丽琼.厦门同安湾和大嶝岛海域 2004 年夏季环境质量现状与评价[J].台湾海峡,2005,(03): 283-288.
369. 庄丽, 陈月琴,蓝藻分子系统学研究进展[J],中山大学学报 ,1999,38(1):74~78.
370. 庄万金.厦门港赤潮发生区溶解氧的时空变化与赤潮生物和环境因子的关系[J].海洋环境科学,1991,(02): 19-25.
2. Adachi, M., Sako, Y., Ishida,Y. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions[J]. J.Phycol, 1996,32:424~432.
3. Ahel, M., R.G. Barlow, R.F.C. Mantoura, Effect of salinity gradients on the distribution of phytoplankton pigments in a stratified estuary[J]. Mar. Eco. Prog. Ser., 1996. 143: 289-295.
4. Amann, R.I., Ludwig, W., Schleifer, k.H., Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiology Review, 1995, 59:143~169.
5. Andersen R. A., Biology and systematics of heterokont and haptophyte algae [J]. J Bot. 2004, 91: 1508-1522.
6. Andersen, R.A., Bidigare, R.R., Keller, M.D., Latasa, M. A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific oceans[J]. Deep-Sea Res. II, 1996, 43:517–537.
7. Anderson, RA, Saunders, GW, Paskind, MP, et al. Ultrastructure and 18S ribosomal-RNA gene sequence for Pelagomonas calceolata genet sp. nov and the description of a new algal class, the Pelagophyceae classis nov[J]. J Phyco.1993.29:701-715.
8. Ansotegui, A., Trigueros, J. M., Orive, E. The use of pigment signatures to assess phytoplankton assemblage structure in estuarine waters[J]. Estuarine Coastal and Shelf Sci. 2001, 52:689-703.
9. Anton A.I., Martinez-Murcia A.J., Rodriguez-Valera F., Sequence diversity in the 16S-23S intergenic spacer region (ISR) of the rRNA operons in representatives of the Escherichia coli ECOR collection[J]. J Mol Evo. 1998, 47: 62~72.
10. Azam F, Fenchel T, Field JG, The ecological role of water column microbes in the sea . Mar Ecol Prog Ser, 1983, 10: 257-263.
11. Babin M., Morel A., Gentili B., Remote sensing of sea surface Sun-induced chlorophyll fluorescence: consequences of natural variations in the optical characteristics of phytoplankton and the quantum yield of chlorophyll a fluorescence[J], J. Remote Sens., 1996,17 (1): 2417–2448.
12. Baldauf, S.L. The deep roots of eukaryotes[J]. Science, 2003, 300: 1703–1706.
13. Barlow, R.G., Cummings, D.G., Gibb, S.W. Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC[J]. Mar. Eco. Pro. Ser., 1997a, 161: 303-307.
14. Barlow, R.G., Mantoura, R.F.C., Cummings, D.G. Pigment chemotaxonomic distributions of phytoplankton during summer in the western Mediterranean[J]. Deep-Sea Res II. 1997b, 44(3-4): 833–850.
15. Barlow, R.G., Mantoura, R.F.C., Gough, M.A. Pigment signatures of the phytoplankton composition in the northeastern Atlantic during the 1990 spring bloom[J]. Deep-Sea Res II,1993, 40:459–477.
16. Barlow, R .G., Mantoura R. F.C., Peinert R., Miller A.E. J., Fileman T.W., Distribution, sedimentation and fate of pigment biomarkers following thermal stratification in the western Alboran Sea[J], Mar. Eco. Pro. Ser., 1995, 125,279–291.
17. Bianchi, T.S., Findlay, S., Dibb, J. Early diagenesis of plant pigments in Husdon River sediments[J]. Estuarine Coastal and Shelf Science. 1993, 36: 517-527.
18. Bianchi, T.S., Findlay, S., Fontvieille, D. Experimental degradation of plant materials in Hudson River sediments. I. Heterotrophic transformations of plant pigments[J]. Biogeochemistry, 1991, 12: 171-187.
19. Bianchi, T.S., Johansson, B., Elmgren, R. Breakdown of phytoplankton pigments in Baltic sediments, effects of anoxia and loss of deposit-feeding macrofauna[J]. J Exp Mar Bio Eco, 2000, 251:161-183.
20. Bianchi, T.S., Kautsky, L., Argyrou, M. Dominant chlorophylls and carotenoids in macroalgae of the Baltic Sea (Baltic Proper): their use as potential biomarkers[J]. Sarsia, 1997, 82:55–62.
21. Bidigare R.R., Kennicutt II M.C., Brooks J.M., Rapid determination of chlorophylls and their degradation products by high-performance liquid chromatography[J], Limnol. Oceanogr., 1985,30 (2): 432–435.
22. Bidigare, R.R., Kennicutte, M.C., Keeney-Kennicutt, A. M. Isolation and purification of chlorophylls a and b for the determination of stable carbon and nitrogen isotope compositions[J]. Anal. Chem., 1991, 63:130-133.
23. Bidigare, R.R., Marra, J., Dickey, T.D. Evidence for phytoplankton succession and chromatic adaptation in the Sargasso Sea during spring 1985. Mar. Ecol. Prog. Ser[J], 1990, 60: 113–122.
24. Biegala IC, Not F, Vaulot D, Simon N. 2003. Quantitative assessment of picoeucaryotes in the natural environment using taxon specific oligonucleotide probes in association with TSA-FISH (Tyramide Signal Amplification - Fluorescent In Situ Hybridization) and flow cytometry[J]. Appl. Environ. Microbiol. 69: 5519-5529.
25. Bowers, H. A., T. Tengs, Glasgow H. B., Jr., Burkholder J. M., Rublee P. A., and Oldach D. W. Development of realtime PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates[J]. Appl. Environ. Microbiol. 2000. 66:4641-4648.
26. Boynton,W.R, Garbaer H, R. Summers, Inputs, transformations and transport of nitrogen and phosphorus in Chesapeake Bay and selected tributaries[J] . Estuaries, 1995.18: 285-314.
27. Bricaud, A., Morel, A., Babin, M. Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (Case 1) Waters: analysis and implications for bio-optical models[J]. J. Geophys. Res., 1998, 103 (C13): 31033–31044.
28. Bricelj,V.M. and Lonsdale, D.J. Aureococcus anophagefferens : Causes and ecological consequences of brown tides in U.S. mid-Atlantic coastal waters[J]. Limnol. Oceanogr. 1997, 42: 1023–1038.
29. Buck, K. R., F. P. Chavez, and L. Campbell. Basin-wide distributions of living carbon components and the inverted trophic pyramid of the central gyre of the North Atlantic Ocean, summer 1993[J]. Aquat. Microb. Ecol. 1996.10: 283–298.
30. Burkill P.H., Mantoura R.F.C., Owens N.J.P. 1993. Biogeochemical cycling In the northwestern Indian Ocean: a brief overview[J]. Deep-Sea Res Ⅱ,40(3): 643~649.
31. Campbell, L., H. A. Nolla, and D. Vaulot, The importance of Prochlorococcus to community structure in the central North Pacific Ocean[J]. Limnol. Oceanogr. 1994,39:954–961.
32. Caron, D. A. (1991) Evolving role of protozoa in aquatic nutrient cycles[M]. In Reid, P. C., Turley, C. M. and Burkhill, P. H. (eds), Protozoa and their Role in Marine Processes. Springer Verlag, Berlin, pp. 387–415.
33. Caroppo C,The contribution of picophytoplankton to community structure in a Mediterranean brackish environment[J]. J Plank Res, 2000 ,22(2):381–397.
34. Casamayor, E.O., Sch?fer, H., Ba?eras, L., Pedrós-Alió, C ., Muyzer, G. Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: a comparison of microscopy and denaturing gradient gel electrophoresis[J]. Appl. Environ. Microbiol. 2000, 66:499–508.
35. Chen, F., Dustman, W.A., Hodson, R.E. Detection of toluene dioxygenase gene and gene expression in Pseudomonas putida FL in a toluene exposed seawater using in situ PCR and hybridization[J]. Hydrobiologia. 1999, 401:131-138.
36. Chen, F., Jose, M.G., Wendy, A.D., Mary, A.M., Robert, E.H. In situ Reverse tanscription: an approach to characterize genetic diversity and activities of prokaryotes[J]. Appl. Environ. Microbiol. 1997, 63(12): 4907~4913.
37. Chen, F., Wendy, D., Mary, A.M., Robert, E.H. In situ PCR methodologies for visualizatioon of microscale genetic and taxonomic diversities of prokaryotic communities[J]. Molecular Microbial Ecology Manual. 1998: 1~17.
38. Claereboudt M.R., Cote J., Bonardelli J.C.Seasonal variation abundance and size structure of phytoplankton in Baiedes chaleurs,southwestern Golf of St. Lawrence, in relation to physical oceanographic conditions[J]. Hydrobiologia, 1995. 306(2): 147~157.
39. Claustre, H., The trophic status of various oceanic provinces as revealed by phytoplankton pigment signatures[J]. Limnol. and Oceanogr,. 1994.39(5): 1206-1210.
40. Claustre, H., Marty, J.C. Evaluation of the utility of chemotaxonomic pigments as a surrogate for particulate DMSP[J]. Limnol. Oceanogr. 2001, 46(4): 989-995.
41. Corey, D.L., Bianchi, T.S., Santschi, P.H. Cross-shelf changes in phytoplankton community composition in the Gulf of Mexico (Texas shelf/slope): the use of plant pigments as biomarkers[J]. Conti. Shelf. Res. 1999, 19: 1-21.
42. Courties, C. Vaquer, A., Troussellier, M., Lautier, J., Chre′tiennot-Dinet, M-J., Neveux, J., Machado, M. C. & Claustre, H., Smallest eukaryotic organism[J]. Nature, 1994.370:255.
43. Coyne, Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard[J], Limnol. Oceanogr.: Methods 3, 2005, 381–391
44. Cullen, D. W., A. K. Lees, I. K. Toth, and J. M. Duncan. Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR[J]. Plant Pathol. 2002.51:281-292.
45. Daugbjerg &. P Henriksen. 2000. Comparative study of. Gymnodinium mikimotoi an. Gymnodinium aureolum, comb. nov. (=Gyrodinium. aureolum) based on morphology, pigment composition. and molecular data[J] J Phycol 36: 394 - 410
46. Daugbjerg, N., and R.A. Andersen. Phylogenetic analysis of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated[J]. Mol. Biol. Evol. 1997. 14:1242-1251.
47. Davis, PG, Caron, DA, Johnson, PW and Sieburth,. J.McN. Phototrophic and apochlorotic components. of picoplankton and nanoplankton[J]. Mar. Ecol. Prog. Ser. , 1985, 21: 15-26
48. Dawson, S. C., and N. R. Pace. Novel kingdom-level eukaryotic diversity in anoxic environments.Proc. Natl. Acad. Sci. 2003.:8324-8329.
49. Dawson, S.C., E.F. DeLong and N.R. Pace Phylogenetic and ecological perspectives on uncultured Crenarchaeota and Korarchaeota[M] In M. Dworkin et al., eds., The Prokaryotes: An Evolving Electronic Database for the Microbiological Community, New York, Springer-Verlag, 2000
50. Delong, E.F., Wickham, G.S., pace, N.R. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells[J]. Science, 1989, 243: 1360~1362.
51. Demers, S., Roy, S., Gagnon, R. Rapid light-induced changes in cell fluorescence and in xanthophyll-cycle pigments of Alexandrium excavatum (Dinophyceae) and Thalassiosira pseudonana (Bacillariophyceae): a photo-protection mechanism[J]. Mar. Ecol. Prog. Ser., 1991, 76: 185–193.
52. Diez, B., Pedro′s-Alio′, C. , Massana, R. Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing[J]. Appl. Environ. Microbiol. 2001a, 67: 2932–2941.
53. Diez, B., Pedrós-Alió, C., Marsh, T L., Massana, R. Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques[J]. Appl. Environ. Microbiol. 2001b, 67:2942–2951.
54. Dragica Radojkovic and Jelena Ku ic , Silver Staining of Denaturing Gradient Gel Electrophoresis Gels[J] , Clinical Chemistry. 2000;46:883-884.
55. Du H. L., Nianzhi Jiao, Yaohua Hu, Yonghui Zeng, Real-Time PCR for Quantification of Aerobic Anoxygenic Phototrophic Bacteria Based on pufM Gene in Marine Environment[J]. Journal of Experimental Marine Biology and Ecology. 2005, 323(2): 95-103.
56. Dugdale, R.C. and J.J. Goering. Uptake of new and regenerated forms of nitrogen in primary productivity[J]. Limnol. Oceanogr. 1967, 12: 196-206.
57. Edgcomb,V.P., Kysela,D.T., Teske,A., de Vera Gomez,A. and Sogin,M.L. Benthic eukaryotic diversity in the Guaymas Basin hydrothermal vent environment[J]. Proc. Natl. Acad. Sci. USA, 2002, 99:7658–7662.
58. Edvardsen, B. and Paasche, E. Bloom dynamics and physiology of Prymnesium and Chrysochromulina[M]. In: Physiological Ecology of Harmful Algal Blooms. NATO ASI Series G, vol.
41. Anderson, D.M., Cembella, A.D., Hallegraeff, G.M. [Eds.], Springer-Verlag, Heidelberg, 1998.pp. 193-208.
59. Eikrem W. and Moestrup . Structural analysis of the flagellar apparatus and the scaly periplast of Chrysochromulina scutellum sp. nov. (Prymnesiopyceae, Haptophyta) from the Skagerrak and the Baltic[M]. Phycologia. 1998, 37: 132-153
60. Everitt, D.A., Wright, S.W., Volkman, J.K., Thomas, D.P., Lindstrom, E.J. Phytoplankton community compositions in the western equatorial Pacific determined from chlorophyll and carotenoid pigment distribution[J]. Deep-Sea Res., 1990, 37: 975-997.
61. Falkowski P.G., LaRoche J., Acclimation to spectral irradiance in algae, J. Appl. Phycol., 27, 8–14.
62. Falkowski, P.G. ,1994. The role of phytoplankton photosynthesis in global biogeochemical cycles[J]. Photosynthesis Research. 1991, 39: 235-258.
63. Ferris, M.J., Ward, D.M. Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis[J]. Appl. Environ.Microbiol., 1997, 63:1375–1381.
64. Finkel Z. V., Physiological basis for environmentally-driven changes in phytoplankton communities[D], 2005, Dissertation, Dalhousie Unnersity, Halifax, Nova Scotia
65. Fisher, S. G., and L. S. Lerman. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with theory[J]. Proc. Natl. Acad. Sci. USA. 1983. 80:1579-1583.
66. Fogg G E. Some comments on picoplankton and its importance in the pelagic ecosystem[J]. Aquat. Microb. Ecol. 1995, 9:33–39.
67. Friedrich A. B., H. Merkert, T. Fendert, J. Hacker, P. Proksch, U. Hentschel, Microbial diversity in the marine sponge Aplysina cavernicola (formerly Verongia cavernicola) analyzed by fluorescence in situ hybridization (FISH)[J], Marine Biology, 1999, 134:461 – 470.
68. Fuhrman. J.A, McCallum,K.,Davia.A.A, Phylogenetic diversity of subsurface marine microbial cimmunities from the Altantic and Pacific oceans[J]. Appl.Environ.Microbiol. 1993.59:1294-1302.
69. Fuller,N. J., Marie D., Partensky F., et al., Clade-specific 16S ribosomal DNA Oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the Red Sea[J]. Appl Environ Microbiol, 2003, 69(5): 2430~2443.
70. Furlong, E., Carpenter S.R. Pigment preservation and remineralization in oxic coastal marine sediments[J]. Geochimica et Cosmochimica Acta, 1988, 52: 87-100.
71. Furuya, K, M. Hayashi, Y. Yabushita,.HPLC Determination of Phytoplankton Pigments Using N,N-Dimethylformamide[J]. J. Oceanogr., 1998. 54,199-203.
72. Furuya, K., Hayashi, M., Yabushita, Y. I. Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures[J]. Deep-Sea Res. II, 2003, 50: 367-387.
73. Galluzzi, L., A. Penna, E. Bertozzini, M. Vila, E. Garces, and M.Magnani. Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate) [J]. Appl. Environ. Microbiol. 2004. 70: 1199-1206.
74. Gao Y-H,Yang XN,Chen X,Wang DZ and Hong LY. Effect of zinic selenium and manganese on the growth of a marine microalga Nannochloropsis oculata (Eusigmatophyceae) [J]. Chin. J. Limnol .Oceanogr., 2002a.20(special issue): 93-101
75. Gao, Y.H., Gao, Y., Jin, H.M., Jiao, N.Z. Extracelluar dissolved organic carbon of a marine nanoplanktonic diatom Chaeltoceros sp.: Influence of daily rhythm, light and temperature[J]. Chin. J. Limnol .Oceanogr., 2002ba, 20: 81-92.
76. Gaul W., A. N. Antia., Taxon-specific growth and selective microzooplankton grazing of phytoplankton in the Northeast Atlantic[J]. J. Mar. Systems., 2001.30: 241-261.
77. Gelsomino, A., Keijzer-Wolters, A.C., Elasas, J.D., Cacco, G. Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. Journal of Microbiological Methods[J]. 1999, 38:1–15.
78. Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., Field, K.G. Genetic diveristy in Sargasso Sea bacterioplankton[J]. Nature, 1990, 345:60~63.
79. Giovannoni, S.J., S. Turner, G.J. Olsen, D.J. Lane, and N.R. Pace. Evolutionary relationships among cyanobacteria and green chloroplasts[J]. J. Bacteriol. 1988, 170:3584-3592.
80. Goericke, R, Welschmeyer, N A. Pigment turnover in the marine diatom Thalassiosira weissflogii. Ⅱ . The 14CO2-labeling kinetics of carotenoids[J]. J. Phycol, 1992, 28: 507-17.
81. Goericke, R., Montoya, J.P. Estimating the contribution of microalgal taxa to chlorophyll a in the field – variations of pigment ratios under nutrient- and light- limited growth[J]. Mar. Ecol. Prog. Ser., 1998, 169: 97–112.
82. Goericke, R., Repeta, D.J. Chlorophylls a and b and divinyl chlorophylls a and b in the open subtropical North Atlantic Ocean[J]. Mar. Ecol. Prog. Ser, 1993, 101: 307–313.
83. Goericke, R., Repeta, D.J. The pigments of Prochlorococcus marinus, The presence of divinyl chlorophyll a and b in a marine prokaryote[J] Limnol. Oceanogr., 1992, 37: 425-433.
84. Gray, M., B. Wawrik, J. Paul, and E. Casper. Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment[J]. Appl. Environ. Microbiol. 2003. 69:5726-5730.
85. Guillou L., Chrétiennot-Dinet M-J, Medlin L.K., Claustre H., Loiseaux-de Go?r S., Vaulot D. Bolidomonas: a new genus with two species belonging to a new algal class, the Bolidophyceae (Heterokonta)[J]. J Phycol .1999a.35: 368-381
86. Guillou L., Moon-van der Staay S.Y., Claustre H, Partensky F., Vaulot D. Diversity and abundance of Bolidophyceae (Heterokonta) in oceanic waters[J]. Appl Environ Microbiol 1999b. 65: 4528-4536
87. Guillou, L., Eikrem, W., Chrétiennot-Dinet, M. J., Le Gall, F., Massana, R., Romari, K., Pedrós-Alió, C. & Vaulot, D. Diversity of picoplanktonic prasinophytes assessed by direct nuclear SSU rDNA sequencing of environmental samples and novel isolates retrieved from oceanic and coastal marine ecosystems[J]. Protist. 2004, 155:193-214
88. Hallegraeff G.M., Jeffrey S.W., 1985, Description of new chlorophyll a alteration products in marine phytoplankton[J], Deep-Sea Res., 32: 697–705.
89. Head, E.J.H., Harris, L.R. Chlorophyll destruction by Calanus grazing on phytoplankton, kinetics, effects of ingestion rate and feeding history, and a mechanistic interpretation[J]. Mar. Ecol. Prog. Ser., 1996, 135:223-235.
90. Head, I.M., Saunders, J.R., Pickup, R.W. Microbial evolution, diversity, and ecology : A decade of ribosomal RNA analysis of uncultivated microorganisms[J]. Microbia. Ecology., 1998, 35: 1–21.
91. Henriksen P., Riemann B., Kaas H., Sorensen H.M., Sorensen H. L., Effect of nutrient-limitation and irradiance on marine phytoplankton pigments, J. Plankton Res., 2002, 24 (9): 835–858.
92. Hess W.R., Weihe A., Loiseaux-de Goer S., Partensky F. and Vaulot D. Characterisation of the single psbA gene of Prochlorococcus marinus CCMP 1375 (Prochlorophyta)[J]. Plant Molecular Biology 27: 1189-1196.
93. Hess, W.H., Partensky, F., Van Der Staay Vaulot, D. Coexistence of phycoerythrin and a chlorophyll a/b antenna in a marine prokaryote[J]. Proc. Nat. Acad. Sci. U.S.A., 1996, 93: 11126~11130.
94. Heukelem L., Lewitus A. J., Kana T.M., Craft N.E., Improved separations of phytoplankton pigments using temperature-controlled high-performance liquid chromatography[J], Mar. Ecol. Prog. Ser., 1994,114: 303–313.
95. Heukelem, L.V., Thomas, S. Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments[J]. J.Chromatogr., 2001, 910: 31- 49.
96. Higgins, H.W., Mackey, D.J. Algal class abundances, estimated from chlorophyll and carotenoid pigments, in the western Equatorial Pacific under El Nino and non-El Nino conditions[J]. Deep-Sea Research I, 2000, 47: 1461–1483.
97. Hodgson, D.A., Wright S. Davies W., N., Mass spectrometry and reverse phase HPLC techniques for the identification of degraded fossil pigments in lake sediments and their application in paleolimnology[J]. J. Paleolimnology 1997, 18: 335-350
98. Huang,B.Q., Hong, H. and Wang, H., 1999, Size-Fractionated Primary Productivity and the Phytoplankton-Bacteria Relationship in the Taiwan Strait[J], Mar. Ecol. Prog. Ser. 183 : 29-38.
99. Hurley, J.P. and D.E. Armstrong., Pigment preservation in lake sediments, a comparison of sedimentary environments in Trout Lake, Wisconsin[J]. J. Fish. Aquat. Sci, 1991,48: 472-486.
100. Janse I, Bok J, and Zwart G. A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis[J]. Journal of Microbiological Methods. 2004, 57:279–281.
101. Jean-Claude M, Jacques C, Marie-Dominique P, Seasonal and interannual dynamics of nutrients and phytoplankton pigments in the western Mediterranean Sea at the DYFAMED time-series station (1991–1999) [J].Deep-Sea Research II,2002, 49 : 1965–1985
102. Jean-Pierre Descy, B. Destasio, G. Gerrish, J. Hood, J.-P. Hurley, A. St. Amand. Pigment ratio and phytoplankton assessment in northern Wisconsin lakes[J], J. Phycol., 2000, 36: 274-286
103. Jeffrey, S.W., Mantoura, R. F. C., Wright, S. W. Phytoplankton Pigments in Oceanography[M]. SCOR, UNESCO Publishing., 1997
104. Jeffrey, S.W., Wright, S.W., Zapata, M. Recent advances in HPLC pigment analysis of phytoplankton[J]. Marine and Freshwater Research, 1999, 50: 879–896.
105. Jensen, S.I., Lewis, R.J., Hoagland, K.D. Genetic variation in Fragilaria capucina clones along a latitudinal gradient[J]. J Phycol, 1995, 31(3):18.
106. Jesus, G.M., Silvia, G.A., Ana, I.A, Francisco, R.V. Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity[J]. Journal of Microbiological Methods, 1999, 36: 55~64.
107. Jiao, N. Z., Sieracki, M.E., Zhang, Y., Du, H.L. Aerobic Anoxygenic Phototrophic Bacteria And Their Roles In Marine Ecosystems[J]. Chin Sci. Bull., 2003a 48(11): 1064-1068.
108. Jiao.,N. Z., Liu, C., Koshikawa, H., Watanabe, M. Dynamics of dimethyl sulfide and dimethyl- sulfoniopropionate in the China Seas[J]. Act. Botan. Sin., 2003b, 45 (7): 774-786.
109. Jiao, N.Z., Wang, R. Ammonium uptake and regeneration fluxes of the microplankton commumities in Jiaozhou Bay[J]. Chin. J. Oceanol. Limnol., 1994, 12(2): 165-174. (A)
110. Jiao, N.Z., Yang, Y. 2002a, Ecological Studies on Prochlorococcus in the China Seas[J], Chin. Sci. Bull., 47 (15): 1243-1250.
111. Jiao, N.Z., Yang, Y.H., Harada, S., Koshigawa, H., Watanabe, M. Responses of Picoplankton to Nutrient Perturbation in the South China Sea With Special Reference to the Coast-ward Distribution of Prochlorococcus[J]. Act. Botan. Sin., 2002b, 44(6): 731-739.
112. Jiao, N.Z., Yang, Y.H., Hong, N., Ma, Y., Koshikawa, H., Watanabe, M. Dyanmics of autotrophic picoplankton and heterotrophic bacteria in the East China Sea[J]. Continental Shelf Research, 2005, 25(7): 856-867.
113. Jiao, N.Z., Yang, Y.H., Koshikawa, H., Watanabe, M. Influence of hydrographic conditions onpicoplankton distribution in the East China Sea, a marginal sea of the Northwest Pacific. Aquatic Microbial Ecology, 2002, 30(1):37-48.
114. Jiao, N.Z., Zhang, Y., Chen, Y. Time series observation based Infrared epifluorescence microscopic approach for accurate enumeration of bacteriochlorophyll containing microbes in Marine Environments[J]. Journal of Microbiological Methods, 2005, 63(10): 1-11.
115. Jochem F.J., Pollehne F., Zeitzschel B. Productivity regime and phytoplankton size structure in the Arabian Sea. Deep-Sea Research Ⅱ , 1993, 40(3): 711~735.
116. Johnsen G , Samset O , Granskog L, In vivo absorption characteristics in 10 classes of bloom-forming phytoplankton: taxonomic characteristics and responses to photoadaptation by means of discriminant and HPLC analysis[J]. Mar. Ecol. Prog. Ser., 1994,105: 149–157.
117. Kim M., W. Philpot . Ocean pigment algorithm based on the phytoplankton particle optics[M]. Ocean Optics XV, Monaco, 2000
118. Knight Jones, EW Aspects of the setting behaviour of larvae Ostrea edulis on Essex oyster beds[J]. Rapp. Cons. Explor. Mer. 1951,128 (II).
119. Kooistra W.H.C.F, Gersonade R, Sims P. Oraginand evolution of the diatoms on 18s rRNA sequences[J]. J Phycol , 1995, 31(3):18.
120. Landry, M.R. and D.L. Kirchman. Microbial community structure and variability in the the tropical Pacific[J]. Deep-Sea Res. II. 2002,49:2669-2693.
121. LaRoche, J., Van Der Staay, G.W.M., Partensky, F., Ducret, A., Aebersold, R., Li, R., Golden, S.S., Hiller, R.G., Wrench, P.M., Larkum, A.D., Green, B. Independent evolution of the Prochlorophyte and green plant chlorophyll a/b light-harvesting proteins[J]. Proc. Nat. Acad. Sci. U.S.A., 1996, 93: 15444~15248.
122. Latasa M., Bidigare R.R., A comparison of phytoplankton populations of the Arabian Sea during the Spring Intermonsoon and Southwest Monsoon of 1995 as described by HPLC-analysed pigments[J], Deep-Sea Res., 1998, 45: 2133–2170.
123. Leeuwe M.A., Stefels J., Effect of iron and light stress on the biochemical composition of the Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition[J], J. Appl. Phycol., 1998, 34: 496–503.
124. Lenaers G. Dinoflagellates in evolution: A molecular phylogenetic analysis of large subunit ribosomal RNA[J]. J. Mol. Evol. 1989, 29: 40-51.
125. Lenaers G. A molecular phylogeny of dinoflagellate protists (Pyrrophyta) inferred from the sequence of 24s rRNA divergent domains D1 and D8[J]. J. Mol. Evol. 1991,32: 53-63.
126. Lerman A, Control on river water composition and the mass balance of river systems[M]. In: Martin J M, Burton J D, Eisma D (eds) , River Inputs to Ocean Systems, Proceedings of a SCOR/ACMRR/ECOR /IAHS/UNESCO /CMG/IABO/IAPSO Review and Workshop, March 26~30, 1979, Rome, Italy, 1981, pp1-4.
127. Lerman, S. and Manski, C. On the Use of Simulated Frequencies to Approximate Choice Probabilities[M], of: Manski, C., and McFadden, D. (eds), Structural Analysis of Discrete Data with Econometric Applications. MIT Press. 1981.
128. Letelier, R.M., Bidigare, R.R., Hebel, D.V. Temporal variability of phytoplankton community structure at the U.S.-JGOFS time-series Station ALOHA based on HPLC pigment analysis[J]. Limn.and Ocean., 1993, 38: 1420-1437.
129. Li, W.K.W. Autotrophic picoplankton in the tropical ocean[J]. Science, 1983, 219: 292-295.
130. Liaaen-Jensen, S., Andrewes, A.G. Analysis of carotenoids and related polyene pigments. Methods in microbiology[M]. G. Gottschalk (ed.)., Acad. Press, New York–London, 1985, 235–283.
131. Lindell, D., and A.F. Post Ultraphytoplankton succession is triggered by deep winter mixing in the Gulf of Aqaba (Eilat), Red Sea[J]. Limnol. Oceanogr. 1995,40(6): 1130-1141
132. Llewellyn C.A. and R.F.C. Mantoura, Pigment biomarkers and particulate carbon in the upper water column compared to the ocean interior of the northeast Atlantic[J]. Deep-Sea Res.I, 1996,43:1165-1184.
133. Lomas MW, PM Glibert, DA Clougherty, DR Huber, Elevated organic nutrient ratios associated with brown tide algal blooms of Aureococcus anophagefferens (Pelagophyceae), Journal of Plankton Research,2001,23 (12) :1339-1344
134. Longhurst and Harrison, The biological pump: Profiles of plankton production and consumption in the upper ocean, Progress in Oceanography 1989, 22: 47-123.
135. Lopez-Garcia, P., F. Rodriguez-Valera, C. Pedros-Alio, and D. Moreira. Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. 2001, Nature 409:603-607.
136. Lu C. K., Chou H.N., Lee C.K., Lee T.N., Lefranc M, Thénot A, Lepère C, Debroas D. Genetic Diversity of Small Eukaryotes in Lakes Differing by Their Trophic Status[J]. Appl. Environ. Microbiol. 2005, 71:5935–5942.
137. Lu, Y Qi, J Goebel, J Zou, Y Gao. Redescription of Prorocentrum donghaiense Lu and comparison with relevant Prorocentrum species[J]. Appl. Ecol. Bull. 2003 14(7):1060
138. Ma Y, Jiao N Z, Molecular ecology of marine Synechococcus[J]. Prog. Natural Sci., 2004,14(8): 649-655
139. Ma, Y., Jiao, N.Z., Zeng, Y.H. Natural Community structure of cyanobacteria in the South China Sea as revealed by rpoC1 gene sequence analysis[J]. Lett. Appl. Microbiol., 2004, 39: 353-358.
140. Mackey, D,J., Higgins, H.W., Mackey, M.D. Algal class abundances in the western equatorial Pacific: estimation from HPLC measurements of chloroplast pigments using CHEMTAX[J]. Deep-Sea Research I, 1998, 45:1441–1468.
141. Mackey, D.J., Blanchot, J., Higgins, H.W., Neveux, J. Phytoplankton abundances and community structure in the equatorial Pacifi[J]c. Deep-Sea Res. II, 2002, 49: 2561-2582.
142. Mackey, D.J., Parslow, J.S., Griffiths, F.B. Phytoplankton productivity and the carbon cycle in the western equatorial Pacific under ENSO and non-ENSO conditions[J]. Deep-Sea Res. II, 1997, 44: 1951-1978.
143. Mackey, M.D., Mackey, D.J., Higgins, H.W. CHEMTAX a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton[J]. Mar. Ecol. Prog. Ser, 1996, 144: 265–283.
144. Majchrowski R., Ostrowska M., Influence of photo- and chromatic acclimation on pigment composition in the sea[J], Oceanologia, 2000, 42 (2): 156-165.
145. Marie D, Brussaard CPD, Partensky F, Vaulot D. Flow cytometric analysis of phytoplankton, bacteria and viruses[M]. Current Protocols in Cytometry, Vol. 11.11 (Robinson JP, ed), pp. 1–15. John Wiley & Sons, New York. 1999.
146. Marie D., Fei Zhu, Vanessa Balagué, Joséphine Ras, & Daniel Vaulot. Eukaryotic picoplankton communities of the Mediterranean Sea in summer assessed by molecular approaches (DGGE, TTGE, QPCR)[J]. FEMS Microbiology Ecology , 2006 , 55(3): 403-415.
147. Marie, D., Simon, N. & Vaulot, D. Phytoplankton cell counting by flow cytometry[M], p. 253-267. In Andersen, R. A. [ed.], Algal Culturing Techniques. Academic Press. 2005.
148. Mark Trice T., M. G. Patricia, L. Chris, , HPLC pigment records provide evidence of past blooms of Aureococcus anophagefferens in the Coastal Bays of Maryland and Virginia[J], USA.Harmful Algae, 2004, 3, 295-304 .
149. Masato H., K. Furuya , H. Hattori, Spatial heterogeneity in distributions of chlorophyll a derivatives in the subarctic north pacific during summer[J]. J. Oceanogr., 2001, 57, 323-331.
150. Massana, R. and K. Jürgens, Composition and population dynamics of planktonic bacteria and bacterivorous flagellates in seawater chemostat cultures[J]. Aquatic Microbial Ecology, 2003, 32: 11-22.
151. Massana, R., BalaguéV, Guillou L, Pedrós-AlióC. Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches[J]. Microbiology Ecology, 2004, 50: 231–243.
152. Massana, R., Guillou, L., D?′ez, B. and Pedros-Alio , C. Unveiling the organisms behind novel eukaryotic ribosomal DNA sequences from the ocean[J]. Appl. Environ. Microbiol. 2002,68: 4554–4558.
153. Medliin E.K., Elwood H.J., Stickel S., et al. Morphological and genetic variation withinn the diatom Skeletonema costatum: evidence for a new species Skeletonema pseudocostatum[J]. J Phycol, 1991, 27:514-524.
154. Meyr-Harms, B. & F. Pollenhe, Alloxanthin in Dinophysis norvegica (Dinophysiales, Dinophyceae ) from the Baltic Sea[J]. J Phycol, 1998, 34: 280-285.
155. Michot B, Hassouna N, Bachellerie JP. Secondary structure of mouse 28S rRNA and general model for the folding of the large rRNA in eukaryotes[J]. Nucleic Acids Res. 1984 May 25;12(10):4259–4279.
156. Mikel, L., Robert, R.B. A compareison of phytoplankton populations of the Arabian Sea during the Spring Intermonsoon and Southwest Monsoon of 1995 as described by HPLC-analyzed pigments[J]. Deep-Sea Res Ⅱ, 1998, 45:2133-2170.
157. Moestrup,. and Thomsen,H.A. (1980) Preparation of shadowcast whole mounts[M]. In Gantt,E. (ed.), Handbook of Phycological Methods: Developmental and Cytological Methods. University Press, Cambridge, Vol. 8, pp. 386–390.
158. Moon-van der Staay, S.Y., De Wachter, R., Vaulot, D. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected-eukaryotic diversity[J]. Nature, 2001, 409: 607–610.
159. Moore LR and SW Chisholm. Photophysiology of the marine cyanobacterium Prochlorococcus: Ecotypic differences among cultured isolates[J]. Limnol. Oceanogr.,1999,44(3):628-638
160. Moore LR, G Rocap, and SW Chisholm. Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes[J]. Nature. 1998,393:464-467
161. Moore, L. R., R. Goericke, and S. W. Chisholm, Comparative physiology of Synechococcus and Prochlorococcus: Influence of light and temperature on growth, pigments, fluorescence and absorptive properties[J], Mar. Ecol. Prog. Ser., 1995, 116: 259-275.
162. Morden CW, Delwiche CF , Kuhsel M , Palmer JD , Gene phylogenies and the endosymbiotic origin of plastids. Biosystems[J] 1992,28 (1 - 3) : 75 - 90.
163. Moreira,D. and Lo′pez-Garc?′a,P. The molecular ecology of microbial eukaryotes unveils a hidden world. Trends in Microbiology [J], 2002, 10:31–38.
164. Morton B., Wu S. S. The hydrology of the coastal waters of Hong Kong[J]. Environmental Research,1975 10, 19~347.
165. Mukai, T., Effects of micro-scale in situ environmental gradients concerning water qualities on the structure of the phytoplankton community in a coastal embayment. Estuarine[J], Coastal Shelf Sci. , 1987, 25: 447-458.
166. Murphy, L.S., Haugen, E.M. The distribution and abundance of phototrophic ultraplankton in the North Atlantic[J]. Limnol. Oceanogr., 1985, 30: 47–58.
167. Murray, A.E., Hollibaugh, J.T. and Orrego, C., Phylogenetic composition of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments[J]. Appl Environ Microbiol., 1996,62:2676~2680.
168. Muyzer, G. DGGE/TGGE, a method for identifying genes from natural communities[J]. Curr. Opin. Microbiol., 1999, 2: 317–322.
169. Muyzer, G., De Waal, E.C., Uitterlinden, A.G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Appl. Environ. Microbiol., 1993, 59: 695–700.
170. Muyzer, G., T. Brinkhoff, U. Nübel, C. Santegoeds, H. Sch?fer, and C. Wawer. Denaturing gradient gel electrophoresis (DGGE) in Microbial Ecology[M]. In G. A. Kowalchuk, F. J. de Bruijn, I. M. Head, A. D. Akkermans, and J. D. van Elsas (ed.), Molecular Microbial Ecology manual, 2nd ed. Kluwer Academic Publishing, London, England, 2004.
171. Nellan, B.A. Identification and phylogenetic analysis of toxigenic Cyanobacteria by multiplex randomly amplified polymorphic DNA PCR[J], Appl Environ Microbiol, 1995, 61: 2286~2291.
172. Neufeld Josh D. and William W. Mohn , Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis[J]. Appl Environ Microbiol. 2005 ,71(8): 4893–4896
173. Not F, Simon N, Biegala I, Vaulot D. Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition[J]. Aquatic Microbial Ecology, 2002, 28: 157-166.
174. Not, F., Latasa, M., Marie, D., Cariou, T., Vaulot, D. & Simon, N, A single species Micromonas pusilla (Prasinophyceae) dominates the eukaryotic picoplankton in the western English Channel[J]. Appl Environ Microbiol, 2004, 70: 4064-4072.
175. Nubel U, Engelen B., Felske A., , Sequence heterogeneities of genes encoding 16S rRNAs in paenibacillus polymyxa detected by temperature gradient gel electrophoresis[J]. Journal of Bacteriology, 1996, 178:5636~5643.
176. Obayashi Y., E. Tanou , Growth and mortality rates of phytoplankton in the northwestern North Pacific estimated by the dilution method and HPLC pigment analysis[J]. J. Exp. Mar. Bio. Eco. 2002, 280 , 33- 52.
177. Odate, T., M. Yanda, H. Mizuta, and Y. Maita. Phytoplankton carbon biomass estimated from thesize fractionated chlorophyll a concentration and cell density in the northern coastal waters from spring bloom tosummer[J]. Bulletin of Plankton Society of Japan, 1993,39: 127-144
178. Olsen, GJ, Lane, DL, Giovannoni, SJ, & Pace, NR . Microbial Ecology and evolution: A ribosomal RNA approach. Ann. Rev. Microbiol[J]., 1986, 40: 337-365.
179. Olson, R.J., Chisholm, S.W., Zettler, E.R. Spatial and temporal distributions of prochlorophyte picoplankton in the North Atlantic Ocean[J]. Deep-Sea Res, 1990, 37: 1033-1051.
180. Pace B, Matthews EA, Johnson KD, Cantor CR, Pace NR. Conserved 5S rRNA complement to tRNA is not required for protein synthesis[J]. Proc Natl Acad Sci U S A. 1982;79(1):36–40.
181. Partensky F, Blanchot J and Vaulot D. Differential distribution of Prochlorococcus and Synechococcus in oceanic waters: a review[M]. In: Charpy L, Larkum A.W.D. (eds), Marine cyanobacteria. Bulletin de l'Institut Océanographique de Monaco No sp. 1999b.19: 457-475
182. Partensky F, Hess WR and Vaulot D. Prochlorococcus, a marine photosynthetic prokaryote of global significance[J]. Microbiology and Molecular Biology Reviews 1999a. 63: 106-127
183. Partensky F., Guillou L. Recent advances in the use of molecular techniques to assess the genetic diversity of marine photosynthetic microorganisms[J]. Genetic diversity of marine picophytoplankton. 1997,47(4):367-374.
184. Perasso, R., Baroin, A., Qu, L. H., Bachellerie, J. P. & Adoutte, A. Origin of the algae. Nature 1989,339:142–4.
185. Pinckney, J.L., Paerl, H.W., Harrington, M.B. Annual cycles of phytoplankton community-structure and bloom dynamics in the Neuse River Estuary, North Carolina [J]. Marine Biology, 1998, 131: 371–381.
186. Pitta, P., Karakassis, I. Size distribution in ultraphytoplankton: a comparative analysis[J]. Environ. Monit. Assess. 2005,102: 85-101.
187. Platl.T., D.V.Subba.Rao and B.Erwin, Photosynthesis of picoplankton in the oligotrophic ocean [J]. Nature, 1983, 301:702~704.
188. Platt, T. , W. G. Harrison, Biogenic fluxes of carbon and oxygen in the ocean [J]. Nature, 1985,318: 55-58.
189. Poryvkina, L, Babichenko, S, Kaitala, S, Spectral fluorescence signatures in characterization of phytoplankton community composition [J]. Journal of Plankton Research,1994,16(10): 1315-1327.
190. Potter D, T C Lajeunesse, G W Saunders, R A Andersen. Convergent evolution masks extensive biodiversity among marine coccoid picoplankton [J]. Biodivers. Conserv. 1997, 6:99–107.
191. Qu HL, Michot B, Bachellerie JP. Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA [J]. Nucleic Acids Res. 1983 Sep 10;11(17):5903–5920.
192. Qu LH, Nicoloso M, Bachellerie JP. Phylogenetic calibration of the 5' terminal domain of large rRNA achieved by determining twenty eucaryotic sequences [J]. J Mol Evol.1988, 28(1-2):113–124.
193. Rappé, M. S., M. T. Suzuki, K. L. Vergin, and S. J. Giovannoni. Phylogenetic diversity of ultraplankton plastid small-subunit rRNA genes recovered in environmental nucleic acid samples from the Pacific and Atlantic coasts of the United States [J]. Appl. Environ. Microbiol. 1998, 64:294-303.
194. Riegman, R, Rowe, A, Nutritional status and pigment composition of phytoplankton during springand summer Phaeocystis blooms in Dutch coastal waters (Marsdiep area) [J]. Neth. J. Sea Res, 1994, 32:13-21
195. Riegman, R., M. Boer, L.D. Domis, Growth of harmful marine algae in multispecies cultures [J]. J. Plankt. Res. 1996, 18:1851-1866.
196. Rocap, G., D. L. Distel, J. B. Waterbury, And S. W. Chisholm. Resolution Of Prochlorococcus and Synechococcus Ecotypes by Using 16S-23S Ribosomal DNA Internal Transcribed Spacer Sequences [J]. Appl. Environ. Microbiol. 2002, 68: 1180-1191.
197. Rodríguez, F., Derelle, E., Guillou, L., Le Gall, F., Vaulot, D. & Moreau, H. Ecotype diversity in the marine picoeukaryote Ostreococcus (Chlorophyta, Prasinophyceae) [J]. Environmental Microbiology, 2005, 7:853-859
198. Romari, K., Vaulot, D. Composition and temporal variability of picoeukaryote communities at a coastal site of the English Channel [J], Limnol. Oceanogr. 2004, 49:784-798
199. Sadoudi, N., Babin, M., Allali, K. Spatial variations of photosynthetic parameters in equatorial Pacific: forcing by vertical mixing and light penetration. Ocean Optics 13, Proc. SPIE, 1996, 2963: 880–885.
200. Santegoeds, C.M., Nold, S.C., Ward, D.M. Denaturing gradient gel electrophoresis used to monitor the enrichment culture of aerobic chemoorganotrophic bacteria from a hot spring cyanobacterial mat [J]. Appl Environ Microbiol, 1996, 62: 3922~3928.
201. Sautour, B.; F. Artigas; A. Herbland & P. Laborde. Zooplankton grazing impact in the plume of dilution of the Gironde estuary (France) prior to the spring bloom [J]. Journal of Plankton Research, Oxford, 1996, 18: 835-853.
202. Scanlan, D.J., Hess, W.R., Partensky, F., Scanlan, J. and Vaulot, D. High degree of genetic variation in Prochlorococcus (Prochlorophyta) revealed by RFLP analysis. European [J] . J Phycol. 1996, 31: 1-9.
203. Schluter, L., Havskum, H. Phytoplankton pigments in relation to carbon content in plankton communities [J]. Mar. Ecol. Prog. Ser., 1997, 155: 55–65.
204. Schluter, L., M?hlenberg, F., Havskum, H., Larsen, S. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas, testing the influence of light and nutrients on pigment/chlorophyll a ratios [J]. Mar. Eco. Pro. Ser., 2000,, 192: 49-63.
205. Shao, P., Chen, Y.Q., Zhou, H., Qu, L.H., Ma, Y., Li, H.Y., Jiao, N.Z. Phylogenetic diversity of Archaea in prawn farm sediment [J]. Marine Biology, 2004, 146 (1): 133-142.
206. Sherr, E.B., and B.F. Sherr. Bacterivory and herbivory: Key roles of phagotrophic protists in pelagic food webs [J]. Microbial Ecology , 1994, 28: 223-235.
207. Sieburth, J.Mc.N., Smetacek, V., Lenz, J. Pelagic ecosystem structure, Heterotrophic compartments of the plankton and their relationship to plankton size fraction [J]. Limnol. Oceanogr., 1978, 23: 1256-1263.
208. Sigler, W.V., Miniaci, C., Zeyer, J. Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure [J]. Journal of Microbiological Methods, 2004, 57: 17–22.
209. Sinha R.P., M. Klisch , A. Groniger, D.-P. Hader, Ultraviloet-absorbing/screening substances in cyanobacteria, phytoplankton and macroalgae [J].J. Photochem. Photobiol, B,Biol. 1998, 47:83-94.
210. Smalla, K., Wieland, G., Buchner, A. Bulk and rhizosphere soil bacterial communities studied bydenaturing gradient gel electro-phoresis:plang dependent enrichment and seasonal shifts revealed [J]. Appl. Environ. Microbiol., 2001, 67: 4742–4751.
211. Stahl, DA. Analysis of hydrothermal vent-associated symbiont by ribosomal RNA sequences [J]. Science. 1984, 224: 409-411.
212. Stoeck,T., Taylor,G.T. and Epstein,S.S. Novel eukaryotes from the permanently anoxic Cariaco Basin (Caribbean Sea) [J]. Appl. Environ. Microbiol. 2003, 69:5656–5663.
213. Stoltes, W. ,G.W. Kraay, A.M. Noordeloos , Genetic and physiological variation in pigment composition of Emiliania Huxleyi (Prymnesiophyceae) and the potential use of its pigment ratios as a quantitative physiological marker [J]. J Phycol, 2000,36:529-539.
214. Ston J, Alicja Kosakowska Qualitative and quantitative analysis of Baltic phytoplankton pigments [J]. Oceanologia 2000, 42 (4): 449-471.
215. Stoń, J. and A. Kosakowska, Qualitative and quantitative analysis of Baltic phytoplankton Pigments [J].,. Oceanologia, 2000, 42 ,4, 449-471.
216. Suzuki M T, M S Rappé, and S J Giovannoni. Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity [J]. Appl. Environ. Microbiol. 1998, 64:4522–4529.
217. Suzuki, K. Minami, C., Liu, H., Saino, T., Temporal and spatial patterns of chemotaxonomic algal pigments in the subarctic Pacific and the Bering Sea during summer 1999 [J]. Deep-Sea Res. II , 2002., 49:5685-5704
218. Suzuki, K., Handa, N. Estimation of phytoplankton succession in a fertilized mesocosm during summer using high-performance liquid chromatographic analysis of pigments [J]. J. Exp. Mar. Bio. Eco.,1997, 214: 1-17.
219. Takahashi M. and P.K.Bienfang. 1983. Size structure of phytoplankton biomass and photosynthesis in subtropical Hawaiian waters [J]. Mar. Biol., 76(2): 203~211.
220. Tang D.L. , D. R. Kester, I-H. Ni, Y.Z. Qi, H. Kawamura., In situ and satellite observations of a harmful algal bloom and water condition at the Pearl River estuary in late autumn 1998 [J]. Harmful Algae. 2003, 2: 89-99.
221. Tengs, T., Dahlberg, O.J. Phylogenetic analyses indicate that the 19’ hexanoyloxy- fucoxanthin- containing dinoflagellates have tertiary plastids of haptophyte origin. Mol. Biol. Evol., 2000, 17(5): 718-729.
222. Thomsen,H.A. A survey of the smallest eukaryotic organisms of the marine phytoplankton[M]. In Platt,T. and Li,W.K.W. (eds), Photosynthetic Picoplankton. Can. Bull. Fish. Aquat. Sci.,1998, 214:121–158.
223. Throndsen,J. Estimating cell numbers[M]. In Hallegraeff,G.M., Anderson,D.M. and Cembella,A.D. (eds), Manual on Harmful Marine Microalgae. IOC Manuals and Guides, 33. UNESCO, Paris, 1995.pp. 63–80.
224. Throndsen,J. and Zingone,A. Micronomads of the Mediterranean Sea[J]. G. Bot. It., 1994.128: 1031–1044.
225. Tolmazin D. Elements of Dynamic Oceanography[M]. Boston, Allen & Unwin. 1985.
226. Tyson GW., Community Structure and Metabolism Through Reconstruction of Microbial Genomes From the Environment [J], Nature, 2004, 428: 37.
227. Urbach, E., Sallie, W.C. Genetic diversity in Prochlorococcus populations flow cytometrically sorted from Sargasso Sea and Gulf Stream [J]. Limnol. Oceanogr. 1998, 43(7): 1615~1630.
228. Vaitomaa, J., and others Quantitative real-time PCR for determination of microcystin synthetase E copy numbers for Microcystis and Anabaena in lakes [J]. Appl. Environ. Microbiol. 2003.69:7289-7297.
229. Van de Peer Y, S Chapelle and R De Wachter, conserved regions of rRNA molecules [J]. Nucleic Acids Res., 1996, 24(17): 3381-3391
230. Van Leeuwe, M A, Stefels, J, Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition [J]. J Phycol, 1998,34: 496–503.
231. Vaulot D. Phytoplankton. Encyclopedia of Life Sciences[M], http://www.els.net, Nature Publishing Group. London 2001.
232. Vaulot, D. and Partensky F., Cell cycle distribution of prochlorophytes in the north western Mediterranean Sea [J]. Deep Sea Res. 1992. 39(5):727-742
233. Veldhuis M. J. W., and Kraay G.W., Vertial distribution and pigment composition of a picoplanktonic prochlorophyte in the subtrophical N Atlantic: a combined study of HPLC-analysis of pigments and flow cytometry [J]. Mar. Ecol. Prog. Ser 1990,68: 121~127
234. Venter,J.C., Remington, K. ,Environmental genome shotgun sequencing of the Sargasso Sea [J]. Science. 2004 ,304(5667):58-60.
235. Verity, P.G., Paffenhofer, G.A., Wallace, D., Sherr, E., Sherr, B., Composition and biomass of plankton in spring on the Cape Hatteras shelf, with implications for carbon flux [J]. Continental Shelf Research, 1996.16, 1087–1116.
236. Verity, P.G., Redalje, D.G., Lohrenz, S.E., Flagg, C., Hristov, R., Coupling between primary production and pelagic consumption in temperate ocean margin pelagic ecosystems [J]. Deep-Sea Research II, 2002. 49 (20): 4553–4569.
237. Waterbury J B. Photosynthetic picoplankton [J]. Can. Bull. F ish. A quat. Sci. , 1986, 214: 71-120
238. Ward, D.M., Ferris, M.J., Nold, S.C., Basteson, M.M.A. natural view of microbial biodiversity within hot spring cyanobacterial communities [J]. Appl. Environ. Microbiol., 1998, 62: 1353–1370.
239. Watts, Betty H. The national workshop on Aboriginal education: Priorities for action and research. Brisbane[M], Australia: University of Queensland Press. 1971.
240. Weber L.H. and S.Z.El-Sayed. 1987. Contributions of the net-.nano-,and picoplankton to the phytoplankton standing crop and primary productivity in the Southern Ocean [J]. J. Plankton Res., 9: 973~994.
241. William J.G.K.,Kubelik AR,Livak K.J. DNA polymorphisms amplified by arbitary primers and ureful as genetic markers [J] Nucleic Acids Res., 1990.18(22): 6531-6535.
242. Wong C. K. ,C. K. Wong, HPLC pigment analysis of marine phytoplankton during a red tide occurrence in Tolo Harbour, Hong Kong Chemosphere .2003.52 ,1633-1640.
243. Worden A.Z., Nolan J.K. , Palenik B. Assessing the dynamics and ecology of marine pico- phytoplankton : The importance of the eukaryotic component [J]. Limnol. Oceanogr. 2004, 49:168– 179.
244. Wright S.W., Jeffrey S.W., Fucoxanthin pigment markers of marine phytoplankton analysed byHPLC and HPTLC [J], Mar. Ecol. Prog. Ser., 1987, 38:259–266.
245. Wright S.W., Shearer J.D., Rapid extraction and high-performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton [J], J. Chromatogr., 1984,294: 281–295.
246. Wright, S W , van den Enden, R L , Stratification/mixing regimes control phytoplankton populations off East Antarctica: evidence from CHEMTAX analysis of HPLC pigment profiles (BROKE survey, Jan–Mar 1996). Deep-Sea Research II,2000, 47: 2363–2400.
247. Wright, S.W., S.W. Jeffrey, F.C. Mantoura, C.A. Llewellyn, T. Bj?rnland, D. Repeta, and N. Welschmeyer . Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton [J]. Mar. Ecol. Prog. Ser. 1991. 77:183-196.
248. Wright, S.W., Thomas, D.P., Marchant, H.J. Analysis of phytoplankton of the Australian sector of the Southern Ocean: comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the ‘CHEMTAX’ matrix factorisation program [J]. Mar. Ecol. Prog. Ser, 1996, 144: 285–298.
249. Wulff A., S.A. Wangberg, Spatial and vertical distribution of phytoplankton pigments in the eastern Atlantic sector of the Southern Ocean [J]., Deep-Sea Res. II 2004, 51:2701-2713.
250. Wyrtki K., Physical Oceanography of the Southeast Asia Waters[M]. NAGA Report,2,1-195, 1961.
251. Yacobi Y. Z., Pollingher U., Gonen Y., Gerhardt V., Sukenik A., HPLC analysis of phytoplankton pigments from Lake Kinneret with special reference to the bloom-forming dinoflagellate Peridinium gatunese (Dinophyceae) and chlorophyll degradation products [J], J. Plankt. Res., 1996, 18 (10): 1781–1796.
252. Yang, Y.H., Jiao, N.Z. Dynamics of picoplankton in the Nansha Islands area of the South China Sea [J] Act. Oceanol. Sin., 2004, 23(3): 493-504.
253. Ypma, Jan Erik & Jahn Throndsen Seasonal dynamics of bacteria, autotrophic picoplankton and small nanoplankton in the Inner Oslofjord and the Skagerrak in 1993 [J]. Sarsia. 1996. 81:57-66.
254. Yuan, J.M, Chen, S., Zhou, P.H., Chen, Y., Qu, L. Genetic diversity of small eukaryotes from the coastal waters of Nansha Islands in China. FEMS Microbiology. Letters [J]. 2004, 240(2):163–170.
255. Zapata, M, Garrido, J L, Occurrence of phytylated chlorophyll c in Isochrysis galbana and Isochrysis sp. (Clone T-ISO) (Prymnesiophyceae) [J]. J Phycol, 1997,33: 209–214.
256. Zapata M, Rodríguez F, Garrido JL. Separation of. chlorophylls and carotenoids from marine phytoplankton:. a new HPLC method using a reversed-phase C8 column. and pyridine-containing mobile phases [J]. Mar Ecol Prog Ser. 2000.195:29–45
257. Zeidner G, Preston CM, Delong EF, Massana R, Post AF, Scanlan DJ, Béjà O, Molecular diversity among marine picophytoplankton as revealed by psbA analyses [J]. Environ Microbiol, 2003,5:212–216
258. Zeng,Y.H., Jiao,N.Z., Cai,H.Y., Chen,X.H., Wei, C.L. Phylogenetic diversity of ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea [J]. Act. Oceanol. Sin., 2004, 23(4): 673-685.
259. Zettler, L. A. A., F. Gomez, E. Zettler, B. G. Keenan, R. Amils, and M. L. Sogin. Eukaryotic diversity in Spain' s River of Fire [J]. Nature .2002. 417:137.
260. Zhu, F., Not, F., Massana, R., Marie, D. & Vaulot, D. Mapping of the picoeucaryotes in marine systems with quantitative PCR of the 18S rRNA gene [J]. FEMS Microbiology Ecology. 2005. 52:79-92
261. 蔡清海,吴善,阮秀凤.厦门西海域盐度的变化[J].热带海洋学报,1993,(3):104~107
262. 蔡昱明,宁修仁,刘子琳, 珠江口初级生产力和新生产力研究[J].海洋学报 , 2002.24(3):101-111.
263. 曹振锐,黄邦钦,刘媛,洪华生,谢廷贵.厦门海域分粒级叶绿素 a 含量的分布特征[J].台湾海峡,2005,(04): 493-498.
264. 陈冠惠、黄荣祥、梁红星等,海洋水文气象 V. 气象和气候,台湾海峡中北部海洋调查研究报告[M],科学出版社,北京,1998
265. 陈怀清,钱树本,1992. 青岛近海微型超微型浮游藻的研究[J]. 海洋学报, 14(3): 105-113.
266. 陈纪新, 黄邦钦, 贾锡伟, 洪华生, 谢廷贵. 利用光合色素研究厦门海域微微型浮游植物群落结构[J]. 海洋环境科学. 2003, 22(3):16–21.
267. 陈金泉,傅子琅,李法西.关于闽南-台湾浅滩渔场上升流的研究[J].台湾海峡,1982,1(2):5-13.
268. 陈敏艺,袁洁,陈月琴,屈良鹄.海洋超微型浮游植物遗传多样性的分子系统学研究进展[J].自然科学进展,2005,(9):1032-1040
269. 陈其焕,陈兴群,张明.厦门港赤潮发生区叶绿素a的分布变化特征.厦门港赤潮调查研究论文集[M].北京:海洋出版社,1993,38-45.
270. 陈瑞祥,林景宏,林茂,戴燕玉.厦门西港浮游动物生态研究[J].台湾海峡,1998,(3): 294-298
271. 陈兴群,陈其焕,张明.厦门东侧水道海域的初级生产力[J].台湾海峡,2002,(2): 217-221
272. 陈兴群,陈其焕,庄亮钟,1989.南海中部叶绿素a分布和光合作用及其与环境因子的关系[J].海洋学报,11(3):349-355.
273. 陈映泰,罗章仁,1991.珠江口现代沉积速率及其反映的沉积特征[J]. 热带海洋,10(2):57-64.
274. 陈于望. 厦门港海域营养状况的分析 .海洋环境科学 [J],1987,(3):14~19.
275. 陈月琴等,南海赤潮有毒甲藻链状-塔玛亚历山大藻的分子鉴定[J],海洋学报,1999, 21(3):106-112
276. 陈宗团,1998. 珠江口陆海相互作用过程中稀土元素生物地球化学[D].博士学位论文,厦门大学,1-183.
277. 程兆第,高亚辉,刘师成.福建沿岸微型硅藻[M].北京:海洋出版社,1993,1-91
278. 程兆第,刘师成.西沙群岛海洋硅藻的研究──Ⅲ.胸隔藻属Mastogloia Thwaites 1新种及其他属种在我国的新记录[J].热带海洋1997,(4):25-31.
279. 杜庆红.厦门同安湾浮游植物变化的初步研究[J] .海洋通报,1996,(6): 20-26
280. 方少华,吕小梅,张跃平.厦门浔江湾小型底栖生物数量分布及生态意义[J] .台湾海峡,2000,(4):474~479
281. 冯士筰,李凤岐,李少菁, 海洋科学导论,[M] pp. 434-501. 高等教育出版社, 北京, 1999.
282. 付子琅.台湾海峡的上升流[J].海洋科学,1984,(2): 52~53.
283. 高亚辉,厦门港微型浮游生物研究[D].理学博士学位论文. 厦门大学. 1990.
284. 高亚辉,程兆第,金德祥.厦门港微型浮游生物叶绿素的分布和作用[J].海洋与湖沼,1994,25(1):87-93.
285. 管秉贤,1997. 海南岛以东外海的暖涡[J].黄渤海海洋,15(4):1-7.
286. 郭沛涌,沈焕庭,张利华.淡水微型浮游植物的FCM研究[J].中国环境科学,2002,(2):101~104
287. 国家海洋局第三海洋研究所. 厦门港湾海洋环境综合调查(第四章海洋生物) [J]. 台湾海峡,1988 ,7 (1) :1-10
288. 韩博平,韩志国,付翔,藻类光合作用机理及模型[M].科学出版社,北京. 2002.
289. 韩舞鹰,马克美, 粤东沿岸上升流的研究[J], 海洋学报, 1988, 8(4): 16-20.
290. 韩舞鹰,马克美, 粤东沿岸上升流氧的各种变化过程[J], 热带海岸,1990,10(3): 65-70.
291. 韩鹰舞,南海海洋化学[M]. 298pp.科学出版社, 北京,1998.
292. 何学佳,厦门西海域浮游植物色素的研究[D] .硕士学位论文, 厦门大学, 2003.
293. 洪华生,戴民汉,黄邦钦等,厦门港浮游植物对磷酸盐吸收速率的研究[J],海洋与湖沼,1994,25(1):54-59;
294. 洪华生,黄邦钦.厦门西海域秋季浮游植物现存量光合作用率的分级研究[J].厦门大学学报 ,1994,33(增刊):12-16.
295. 洪华生,商少凌,张彩云,黄邦钦,胡建宇,黄加祺,卢振彬.台湾海峡生态系统对海洋环境年际变动的响应分析[J].海洋学报 ,2005,(2): 63-68
296. 洪华生等,闽南-台湾浅滩渔场上升流区生态系研究[J],科学出版社, 北京, 1991
297. 洪鹰,李立.台湾海峡南部及附近海域夏季的陆架-陆坡锋[J].台湾海峡,1999,(2):159-162
298. 胡建宇,洪华生,贺志刚等,1998年8月台湾海峡表层温、盐度分析[J],热带海洋,2000,19(4):15-22
299. 黄邦钦,洪华生,柯林,曹振锐,2005,珠江口分粒级浮游植物叶绿 a 和初级生产力研究[J], 海洋学报,27(6):180-185.
300. 黄邦钦,洪华生,王大志,林学举,张钒,刘媛.台湾海峡浮游植物生物量和初级生产力的粒级结构及碳流途径[J].台湾海峡,2002,21(1):23-30.
301. 黄邦钦,洪华生,薛雄志.厦门西海域水体中碱性磷酸酶活力分布及其影响因子分析[J] .海洋学报 ,2000,(1): 62-68.
302. 黄邦钦,黄世玉,翁妍,洪华生.溶解态磷在海洋微藻碱性磷酸酶活力变化中的调控作用[J] .海洋学报 ,1999,(1): 55-60.
303. 黄邦钦,林学举.厦门西侧海域超微型浮游植物的时空分布及其调控机制[J],台湾海峡,2000,19(3):329–336.
304. 黄邦钦,王海黎,洪华生,商少凌.厦门海域浮游植物和细菌对溶解有机磷的利用[J].厦门大学学报 ,1996,(04): 625-630.
305. 黄良民,1992.南海不同海区叶绿素a和海水荧光值的垂向变化[J].热带海洋,11(4):89-95.
306. 黄良民,1992.珠江口水域叶绿素a和类胡罗卜素的周年分布[J].海洋环境科学,11(2): 13-18.
307. 黄良民,陈清潮,黄创俭,朱嘉濠等, 1994, 珠江口内海叶绿素a分布与环境因素关系[J], 生态学报,14(增刊):22-27.
308. 黄良民,陈清潮,林永水,南海北部海区浮游生物生产力分布初探[J].热带海洋研究, 1997, 5: 44-53.
309. 黄良民,陈清潮,尹建强,温伟英等, 珠江口及其邻近海域环境动态与基础生物结构初探[J]. 海洋环境科学, 1997.16(3): 1-7.
310. 黄小平,黄良民, 珠江口海域无机氮和活性磷酸盐含量的时空变化特征[J]. 台湾海峡, 2002. 21: 416-421.
311. 焦念志,陈念红.原绿球藻──海洋生态学研究的新领域[J].海洋科学,1995,(4):9-12
312. 焦念志,杨燕辉.中国海原绿球藻研究[J].科学通报,2002,(7):485-491.
313. 金德祥.厦门港1954年浮游生物的定量调查[J].厦门大学学报, 1955,5:17-30.
314. 蓝先洪. 珠江口沉积物的地球化学研究.见:张 经.中国主要河口的主物地球化学研究— 化学物质的迁移与环境[M] 北京:海洋出版社. 1996.
315. 李超伦,栾凤鹤.东海春季真光层分级叶绿素a分布特点的初步研究[J].海洋科学,1998,(4):59-62.
316. 李立, 南海上层环流观测研究进展[J]. 台湾海峡. 2002, 21: 114-125
317. 李立,郭小钢,吴日升.台湾海峡南部的海洋锋[J].台湾海峡,2000,(2):147-152.
318. 李立,李达.台湾浅滩西侧水道夏季的水文特征与上升流[J].台湾海峡,1989,8(4):353-359.
319. 李文权,厦门海沧沿岸水域初级生产力及其与环境的关系[J],热带海洋,1999,(3): 51-57.
320. 李雅琴,程兆第,金德祥.厦门港浮游硅藻生态的研究[J].厦门大学学报 ,1990,29(3):358-360.
321. 林更铭,杨清浪,林金美.厦门岛周围海域浮游植物的种类组成及丰度[J].台湾海峡,1994,13(4):3583-3584.
322. 林更铭,杨清浪,林金美.厦门岛周围海域浮游植物与环境因子的关系[J].海洋通报,1993,12(6):40-453.
323. 林洪瑛,韩舞鹰,王汉奎,程赛伟.南沙群岛海域溶解氧垂直分布最大值的季节特征[J].海洋学报,2001,(5): 51-58.
324. 林金美.厦门附近海域浮游甲藻类的分布. 生态学报,1990,(2): 21-26.
325. 林学举,黄邦钦,洪华生,王大志,肖天.东、黄海典型海域叶绿素 a 含量的垂向变化与周日波动[J].海洋科学,2002,(11): 57-63.
326. 刘仁沿,傅云娜,贺广凯,孙育红,孙茜,尚龙生,赵冬至.HPLC 方法分析表层海水叶绿素 a 含量[J]. 海洋通报,2004,(4): 75-79.
327. 刘媛,黄邦钦,曹振锐,洪华生.厦门海域春夏季微型浮游动物对浮游植物的摄食压力初探 .海洋环境科学,2005,(1): 1-12.
328. 卢振彬,戴泉水,颜尤明.台湾海峡及其邻近海域渔业资源生产力和最大持续产量.中国水产科学,2002,(01): 28-32.
329. 陆斗定,齐雨藻, Jeanette Goebel, 邹景忠,高亚辉.东海原甲藻修订及与相关原甲藻的分类学比较.应用生态学报,2003,(7): 1060-1065.
330. 罗冬莲.同安湾浮游动物数量的平面分布和季节变动 .福建水产,2004,(2).:83-87.
331. 宁修仁,海洋微型和超微型浮游生物.东海海洋,1997,15(3):60~64.
332. 宁修仁、史君贤、刘子彬、蔡昱明. 1996. 南大洋蓝细菌和微微型光合真核生物的丰度与分布. 中国科学(C辑),26(2):164-171.
333. 宁修仁,C.库蒂.长江口及冲淡水区叶绿素 a、细菌、ATP、POC 及微生物呼吸作用速率之间的关系[J].海洋学报,1991,(6):831-837.
334. 宁修仁,刘子琳,史君贤.渤、黄、东海初级生产力和潜在渔业生产量的评估[J].海洋学报 ,1995,(3):72-84.
335. 宁修仁,刘子琳,蔡昱明.我国海洋初级生产力研究二十年[J].东海海洋,2000,(3): 13-20.
336. 宁修仁,刘子琳,蔡昱明.象山港潮滩底栖微型藻类现存量和初级生产力[J].海洋学报 ,1999,(3):82-87.
337. 彭兴跃,洪海征,黄明等.厦门港海水光合色素特征[J].台湾海峡,2002,21(1):79-84.
338. 齐雨藻,中国沿海赤潮[M], p179, 科学出版社, 北京, 2003.
339. 屈良鹄 ,陈 月琴 .简单并 不代 表原 始── 关于生 物早期演 化的 一种 新观点[J].中 山大 学学报 ,1999,(1):128-129.
340. 商少凌,洪华生.厦门西海域环境参数的分布特征[J].厦门大学学报 ,1994. 33(增刊):97—101.
341. 邵鹏,袁洁,陈月琴,自然水样微型藻类遗传多样性方法学研究[J],海洋科学,2002,26(4):1~4.
342. 王海黎,洪华生,徐立.反相液相色谱法分离、测定海洋浮游植物的叶绿素和类胡萝卜素[J].海洋科学,1999,4:6-9.
343. 王海黎,洪华生.近岸海域光合色素的生物标志物研究Ⅰ台湾海峡特征光合色素的分布及其对浮游植物类群结构的指示[J],海洋学报,2000,22(3):94-102.
344. 王和勇,陈敏,廖志华,孙敏,RFLP、RAPD、AFLP 分子标记及其在植物生物技术中的应用[J],生物学杂志,1999,16(4):24~25.
345. 王寿景,李立,李燕初,陈季良,吴日升.厦门浔江湾海洋水文特征[J] .台湾海峡,1998,6(2): 201-206
346. 王伟强,张远辉,黄自强,九龙江口-厦门港河口盐度锋面的特征[J],台湾海峡,2000,19(1): 82-88.
347. 吴日升,李立.南海上升流研究概述.台湾海峡,2003,(2): 262-266.
348. 厦门海岛资源综合调查领导小组办公室等,厦门市海岛资源综合调查研究报告[M],北京:海洋出版社,1996, pp107-111.
349. 肖晖,郭小钢,吴日升.台湾海峡水文特征研究概述[J].台湾海峡,2002,(1):126-138.
350. 肖晖,台湾海峡西部沿岸上升流的研究[J],台湾海峡,1988,7(2): 141-142.
351. 徐锡祯, 南海水平环流概述[J], 中国海洋湖沼学会水文气象学会会议论文集, 科学出版社, 1980. 137-145.
352. 阎俊岳,陈乾金,张秀芝,黄爱芬, 中国近海气候[M]. 北京:科学出版社.1993, pp.109— 280.
353. 颜天,周名江,邹景忠等,香港及珠江口海域有害赤潮发生机制初步探讨[J]. 生态学报, 2001. 21(10): 1634-1641.
354. 杨海军,刘秦玉,南海海洋环流研究综述. 地球科学进展[J], 1998.13(4): 364-368.
355. 杨纪明.世界海洋渔业资源[J].海洋科学,1979,(2):112-117.
356. 杨清良,林更铭,蔡秉及.厦门东侧海域浮游植物的种类组成与分布[J].台湾海峡,2000,(3):337-341.
357. 杨 清 良 , 林 更铭 , 林金 美 厦门文 昌鱼 自然 保护 区 周围 海域 浮游植物 的生 态 [J]. 台湾海峡,1993,12(3):205-2172.
358. 杨清良,林更铭.厦门市海岛资源综合调查研究报告(第十章第三节浮游生物).厦门市海岛资源综合调查研究报告[J].北京:海洋出版社,1996.112-118.
359. 于大江主编,近海资源保护与可持续利用[J],北京:海洋出版社 2001.
360. 袁洁,邵鹏,陈月琴,蔡创华,屈良鹄.南沙群岛微型与超微型真核藻类遗传多样性的初步研究[J].海洋科学,2003,(7): 43-47.
361. 袁明, 珠温柔, 刘吉升等. 几种植物材料中总RNA的提取[J]. 分子植物育种. 2005, 3(2):285–292.
362. 曾刚.福建近海沿岸水及其水文状况[J].海洋通报,1986,(3): 32-37.
363. 张利华,流式细胞术在海洋浮游植物研究中的应用[J],东海海洋,1998,16(1):59~63.
364. 张学成主编,海藻遗传学[M],中国农业出版社,2005.
365. 赵焕庭等,珠江口评价[M]. 北京:海洋出版社,1990.
366. 周玉琴,厦门西港海域水质污染状况分析[J]. 海洋环境科学,1998,(4): 59-63.
367. 朱根海等. 南海浮游植物种类组成和丰度分布的研究[J].海洋学报,2003,25(2):8-23.
368. 庄宏儒,吕小梅,蔡励勋,黄槐,林情员,蒋龙成,沈丽琼.厦门同安湾和大嶝岛海域 2004 年夏季环境质量现状与评价[J].台湾海峡,2005,(03): 283-288.
369. 庄丽, 陈月琴,蓝藻分子系统学研究进展[J],中山大学学报 ,1999,38(1):74~78.
370. 庄万金.厦门港赤潮发生区溶解氧的时空变化与赤潮生物和环境因子的关系[J].海洋环境科学,1991,(02): 19-25.