PGRs对紫花苜蓿苗建成及水分胁迫的调控效应
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摘要
试验于2008年在黑龙江八一农垦大学林甸县吉祥村试验基地和农学实验室进行。以紫花苜蓿(Medicago sativa L.)(龙牡801、草原1号)为试验材料,SODM、DTA-6、CC、DPC四种植物生长调节剂的梯度溶液为试剂,对紫花苜蓿浸种8h后播种,清水浸种为CK,通过室外庭院框栽砂培、室内垂直板和棚室内盆栽三种试验研究方法,研究了PGRs对紫花苜蓿苗建成的影响及水分胁迫的调控效应,得出了以下结论:
室外框栽沙培法表明:应用四种调节剂的梯度溶液,采取浸种法播种后,紫花苜蓿出苗率产生了不同的促进和抑制影响,四种调节剂中只有DPC在高浓度400mg/L、200mg/L下对紫花苜出苗率具有显著抑制效应(P<0.01),不利促苗;综合形态学和生理学指标,调节剂SODM:15mg/L、DTA-6:30mg/L、氯化胆碱(CC):25 mg/L、缩节胺(DPC):25 mg/L具有最佳的苗促进效应,表现出最佳的苗建成效果。
在四种植物生长调节剂的浸种处理条件下,苜蓿的株高、根长、地上物质积累、地下物质积累均表现出了浓度效应,高浓度普遍表现为抑制作用,同时发现DPC、CC在浓度400mg/L、DTA-6在浓度120mg/L时,对苜蓿幼苗药害严重。
垂直板法研究结果表明:与对照比较,调节剂氯化胆碱(CC)浓度为200mg/L时对苜蓿苗建成的主根粗、株高、根长有最佳的抑制效应;而浓度为25 mg/L的调节剂水平对苜蓿苗物质的积累有显著的抑制作用。调节剂SODM浓度为15mg/L时对苜蓿苗建成的主根粗、株高、根长有显著的促进作用;浓度为15mg/L时对苜蓿苗建成的物质积累有显著的促进作用。调节剂(DTA-6)浓度为30mg/L时对苜蓿苗建成的主根粗、株高、根长及物质的积累有显著的促进作用;调节剂缩节胺(DPC)浓度为25mg/L时对苜蓿苗建成的主根粗、株高、根长有最佳的调控效应。
在盆栽干旱条件下,结果表明:在多数测量指标中,草原1号优于龙牧801,表现优异,体现了其优良的抗逆境品质。各植物生长调节剂处理中,经氯化胆碱处理的苜蓿在持续的水分亏缺下植株株高、根长、干物质积累显著增加,叶绿素持有率高,游离脯氨酸维持积累量较高,表现了氯化胆碱具有良好的增加苜蓿抗逆性的效果;另外3种植物生长调节剂也表现出良好的影响,DPC显著促进两品种的株高增加和茎粗增长,促进龙牧801根系活力增加;DTA-6显著促进两品种根系脯氨酸含量和草原1号根长增加; SODM显著促进两品种根系脯氨酸含量增加、茎粗增加、草原1号根长增长,龙牧801根系活力增加。
在盆栽淹水条件下,结果表明:就苜蓿品种而言,草原1号在多数测量指标中表现优异,体现了其优良的抗水淹性能。各植物生长调节剂处理中,经SODM处理的植株在持续的在水淹胁迫条件下茎粗、株高、鲜重有良好表现,叶绿素持有率高,游离脯氨酸维持积累时间较长,维持积累量较高,均比在正常水分条件下表现优异,表现了SODM良好的增加苜蓿抗逆性的效果。DPC显著促进两苜蓿品种的脯氨酸和茎粗增长,DTA-6显著促进两品种根系脯氨酸、叶绿素含量和草原1号的茎粗,CC显著促进草原1号品种的茎粗和脯氨酸的含量。
在水分胁迫试验中,各种适宜浓度调节剂以SODM调控旱、涝效果最好,但调控效果对苜蓿品种间无显著性差异。
Test in 2008 in Heilongjiang province Lindian County auspicious test base village and agronomic laboratories. In alfalfa (Medicago sativa L.) (Longmu 801, Prairie No. 1) as the test material, SODM, DTA-6, CC, DPC four plant growth regulators gradient solution as reagent, on alfalfa seed after soaking 8h , water soaking as CK, outdoor yard box by planting sand culture, indoor potted plants, vertical panels and roof are three experimental methods to study the PGRs on the completion of alfalfa seedlings and regulatory effects of water stress, reached the following conclusions:
Outdoor planting in sand box method showed: the gradient of the solution of four regulators to take after sowing by seed, seedling alfalfa produced different promotion and inhibition effects of the four regulators, only DPC at high concentrations 400mg / L, 200mg / L Alfalfa seedling emergence under significant inhibitory effect (P <0.01), negative and promote seedling; integrated morphological and physiological indicators, regulators SODM: 15mg / L, DTA-6: 30mg / L, choline chloride (CC): 25 mg / L, DPC (DPC): 25 mg / L has the best seedlings for effect, showing the best of the seedlings into effect.
In the four plant growth regulators soaking conditions, the alfalfa plant height, root length, surface material of the accumulation of ground substance accumulation and concentration effect shown, in general showed high concentrations inhibited also found that the DPC, CC in the concentration 400mg / L, DTA-6 at a concentration of 120mg / L, the severe phytotoxicity on alfalfa seedlings.
The results show that the vertical plate: comparison with the control, regulator of choline chloride (CC) concentration of 200mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length has the best inhibitory effect; while concentrations of 25 mg / L, the level of regulator substances on the accumulation of alfalfa seedlings significantly inhibited. Regulators SODM concentration of 15mg / L when alfalfa seedlings into the main root diameter, plant height, root length significantly promoted; concentration of 15mg / L when the material accumulation of alfalfa seedlings established a significant role in promoting. Regulator (DTA-6) concentration of 30mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length and the accumulation of a significant role in promoting; regulator of DPC (DPC) concentration of 25mg / L when completion of the main root of the alfalfa seedlings rough, plant height, root length has the best control effect.
In plants under drought conditions, results showed that: in most metrics, the grassland than 1 Longmu 801, outstanding performance, reflecting its excellent anti-stress quality. The plant growth regulator treatments, choline chloride treatment of alfalfa by continuing under water deficit in plant height, root length, dry matter accumulation increased significantly, holding high chlorophyll, free proline accumulation than the maintenance of high, showing an increase of choline chloride has a good effect of alfalfa resistance; other three kinds of plant growth regulators also showed a good effect, DPC was significantly increased for two species of plant height and stem diameter growth, and promote long grazing 801 root activity increased; DTA-6 significantly promoted the two cultivars, proline content and root length increased grassland 1; SODM significant for the two cultivars, proline content increased, stem diameter increased root length growth of grassland on the 1st, Long Mu 801 root activity increased.
Flooding conditions in the pot, the results show that: on alfalfa, the grasslands on the 1st measurement indicators in the most outstanding performance reflects the performance of its excellent resistance to flooding. The plant growth regulators in plants treated by SODM sustained stress conditions in the flooded stem diameter, plant height, fresh weight to perform well, holding high chlorophyll, free proline accumulation to maintain a longer time accumulation to maintain higher than normal water conditions in the outstanding performance, the performance of the SODM good resistance to increase the effect of alfalfa. DPC significantly promote the two alfalfa varieties and stem diameter growth of proline, DTA-6 significantly promoted the two cultivars, proline, chlorophyll content and grassland on the 1st of stem diameter, CC significantly promote the grassland species of stem diameter and 1 prolyl acid.
In the water stress experiment, the concentration of a variety of appropriate control regulator to SODM drought and flood the best, but the regulatory effects on alfalfa was no significant difference.
室外框栽沙培法表明:应用四种调节剂的梯度溶液,采取浸种法播种后,紫花苜蓿出苗率产生了不同的促进和抑制影响,四种调节剂中只有DPC在高浓度400mg/L、200mg/L下对紫花苜出苗率具有显著抑制效应(P<0.01),不利促苗;综合形态学和生理学指标,调节剂SODM:15mg/L、DTA-6:30mg/L、氯化胆碱(CC):25 mg/L、缩节胺(DPC):25 mg/L具有最佳的苗促进效应,表现出最佳的苗建成效果。
在四种植物生长调节剂的浸种处理条件下,苜蓿的株高、根长、地上物质积累、地下物质积累均表现出了浓度效应,高浓度普遍表现为抑制作用,同时发现DPC、CC在浓度400mg/L、DTA-6在浓度120mg/L时,对苜蓿幼苗药害严重。
垂直板法研究结果表明:与对照比较,调节剂氯化胆碱(CC)浓度为200mg/L时对苜蓿苗建成的主根粗、株高、根长有最佳的抑制效应;而浓度为25 mg/L的调节剂水平对苜蓿苗物质的积累有显著的抑制作用。调节剂SODM浓度为15mg/L时对苜蓿苗建成的主根粗、株高、根长有显著的促进作用;浓度为15mg/L时对苜蓿苗建成的物质积累有显著的促进作用。调节剂(DTA-6)浓度为30mg/L时对苜蓿苗建成的主根粗、株高、根长及物质的积累有显著的促进作用;调节剂缩节胺(DPC)浓度为25mg/L时对苜蓿苗建成的主根粗、株高、根长有最佳的调控效应。
在盆栽干旱条件下,结果表明:在多数测量指标中,草原1号优于龙牧801,表现优异,体现了其优良的抗逆境品质。各植物生长调节剂处理中,经氯化胆碱处理的苜蓿在持续的水分亏缺下植株株高、根长、干物质积累显著增加,叶绿素持有率高,游离脯氨酸维持积累量较高,表现了氯化胆碱具有良好的增加苜蓿抗逆性的效果;另外3种植物生长调节剂也表现出良好的影响,DPC显著促进两品种的株高增加和茎粗增长,促进龙牧801根系活力增加;DTA-6显著促进两品种根系脯氨酸含量和草原1号根长增加; SODM显著促进两品种根系脯氨酸含量增加、茎粗增加、草原1号根长增长,龙牧801根系活力增加。
在盆栽淹水条件下,结果表明:就苜蓿品种而言,草原1号在多数测量指标中表现优异,体现了其优良的抗水淹性能。各植物生长调节剂处理中,经SODM处理的植株在持续的在水淹胁迫条件下茎粗、株高、鲜重有良好表现,叶绿素持有率高,游离脯氨酸维持积累时间较长,维持积累量较高,均比在正常水分条件下表现优异,表现了SODM良好的增加苜蓿抗逆性的效果。DPC显著促进两苜蓿品种的脯氨酸和茎粗增长,DTA-6显著促进两品种根系脯氨酸、叶绿素含量和草原1号的茎粗,CC显著促进草原1号品种的茎粗和脯氨酸的含量。
在水分胁迫试验中,各种适宜浓度调节剂以SODM调控旱、涝效果最好,但调控效果对苜蓿品种间无显著性差异。
Test in 2008 in Heilongjiang province Lindian County auspicious test base village and agronomic laboratories. In alfalfa (Medicago sativa L.) (Longmu 801, Prairie No. 1) as the test material, SODM, DTA-6, CC, DPC four plant growth regulators gradient solution as reagent, on alfalfa seed after soaking 8h , water soaking as CK, outdoor yard box by planting sand culture, indoor potted plants, vertical panels and roof are three experimental methods to study the PGRs on the completion of alfalfa seedlings and regulatory effects of water stress, reached the following conclusions:
Outdoor planting in sand box method showed: the gradient of the solution of four regulators to take after sowing by seed, seedling alfalfa produced different promotion and inhibition effects of the four regulators, only DPC at high concentrations 400mg / L, 200mg / L Alfalfa seedling emergence under significant inhibitory effect (P <0.01), negative and promote seedling; integrated morphological and physiological indicators, regulators SODM: 15mg / L, DTA-6: 30mg / L, choline chloride (CC): 25 mg / L, DPC (DPC): 25 mg / L has the best seedlings for effect, showing the best of the seedlings into effect.
In the four plant growth regulators soaking conditions, the alfalfa plant height, root length, surface material of the accumulation of ground substance accumulation and concentration effect shown, in general showed high concentrations inhibited also found that the DPC, CC in the concentration 400mg / L, DTA-6 at a concentration of 120mg / L, the severe phytotoxicity on alfalfa seedlings.
The results show that the vertical plate: comparison with the control, regulator of choline chloride (CC) concentration of 200mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length has the best inhibitory effect; while concentrations of 25 mg / L, the level of regulator substances on the accumulation of alfalfa seedlings significantly inhibited. Regulators SODM concentration of 15mg / L when alfalfa seedlings into the main root diameter, plant height, root length significantly promoted; concentration of 15mg / L when the material accumulation of alfalfa seedlings established a significant role in promoting. Regulator (DTA-6) concentration of 30mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length and the accumulation of a significant role in promoting; regulator of DPC (DPC) concentration of 25mg / L when completion of the main root of the alfalfa seedlings rough, plant height, root length has the best control effect.
In plants under drought conditions, results showed that: in most metrics, the grassland than 1 Longmu 801, outstanding performance, reflecting its excellent anti-stress quality. The plant growth regulator treatments, choline chloride treatment of alfalfa by continuing under water deficit in plant height, root length, dry matter accumulation increased significantly, holding high chlorophyll, free proline accumulation than the maintenance of high, showing an increase of choline chloride has a good effect of alfalfa resistance; other three kinds of plant growth regulators also showed a good effect, DPC was significantly increased for two species of plant height and stem diameter growth, and promote long grazing 801 root activity increased; DTA-6 significantly promoted the two cultivars, proline content and root length increased grassland 1; SODM significant for the two cultivars, proline content increased, stem diameter increased root length growth of grassland on the 1st, Long Mu 801 root activity increased.
Flooding conditions in the pot, the results show that: on alfalfa, the grasslands on the 1st measurement indicators in the most outstanding performance reflects the performance of its excellent resistance to flooding. The plant growth regulators in plants treated by SODM sustained stress conditions in the flooded stem diameter, plant height, fresh weight to perform well, holding high chlorophyll, free proline accumulation to maintain a longer time accumulation to maintain higher than normal water conditions in the outstanding performance, the performance of the SODM good resistance to increase the effect of alfalfa. DPC significantly promote the two alfalfa varieties and stem diameter growth of proline, DTA-6 significantly promoted the two cultivars, proline, chlorophyll content and grassland on the 1st of stem diameter, CC significantly promote the grassland species of stem diameter and 1 prolyl acid.
In the water stress experiment, the concentration of a variety of appropriate control regulator to SODM drought and flood the best, but the regulatory effects on alfalfa was no significant difference.
引文
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