细胞间黏附分子-1和白介素-10与白癜风的相关性研究
摘要
目的:探讨可溶性细胞间黏分子-1(sICAM-1)和白介素-10(IL-10)与湖南省衡阳地区汉族人群白癜风发病的相关性及与病情活动的关系,为白癜风的病因学研究和治疗提供新的理论依据。
方法:选择湖南省衡阳地区汉族人群白癜风患者60名和40名健康志愿者为研究对象,采肘静脉血4毫升,取血浆,应用ELISA法检测血浆中sICAM-1及IL-10的浓度。
结果:①白癜风患者血浆中sICAM-1的浓度明显高于健康对照组(P<0.01)进展期与泛发型白癜风患者血浆sICAM-1的浓度分别显著高于稳定期和局限型患者(P<0.01),而稳定期与局限型白癜风患者血浆中sICAM-1的浓度与健康对照组无差异(P>0.05)。②白癜风患者血浆中IL-10的浓度明显低于健康对照组(P<0.01),进展期与泛发型白癜风患者血浆中IL-10的浓度分别低于稳定期与局限型患者(P<0.01,P<0.05),而稳定期与局限型白癜风患者血浆中IL-10的浓度与健康对照组无差异(P>0.05)。③白癜风患者组血浆中的sICAM-1与IL-10的浓度呈负相关(r=-0.37,p=0.042),且进展期及泛发型白癜风患者血浆中sICAM-1上升的浓度与IL-10下降的浓度有明显负相关关系(r=-0.82, p=0.001,r=-0.49,p=0.002)。
结论:1白癜风患者血浆中sICAM-1和IL-10表达异常,表明二者可能参与了白癜风的发病。
2在白癜风病情活动时,sICAM-1与IL-10呈负相关。
Objectives: To explore the relationship between intercellular adhesion molecule-1 and Interieukin-10 among Han people in Hengyang of Hunan province with vitiligo, and to provide the new theory and clinical evidence for vitiligo treatment methods.
Methods: A enzyme-linked immuno sorbent assay was used to detect the levels of s ICAM -1 and IL - 10 in plasma by collecting cubital vein blood of 60 vitiligo patients and 40 normal controls among Han people in Hengyang of Hunan province.
Results:①The plasma level of sICAM -1 in vitiligo is higher than Healthy controls(P<0.01). The level of sICAM -1 in the plasma of patients with progressive and diffuse vitiligo was significantiy higher than the stable and localized vitiligo(P<0.01). No significant difference was obsevent(P > 0. 05) between in sera sICAM -1 level with stable and localized vitiligo and the healthy control.②The level of IL-10 in the plasma of patients with vitiligo was obviously belower than the healthy controls(P<0.01). The level of IL-10 in the plasma of patients with progressive and diffuse vitiligo was obviously belower than the stable and localized vitiligo(P<0.01,P<0.05).No obviously difference was obsevent in sera IL-10 level between patients with stable and localized vitiligo and the healthy control(P > 0. 05).③Negative correlation between sICAM-1and IL-10 was obsevent in progressive and diffuse vitiligo.The changes of sICAM -1 was significantiy associated with The changes of IL-10.
Conclusion: 1 vitiligo is associated with abnormal expression of t sICAM-1and IL-10 in serum, it indicats that they are involved in vitiligo.
2 sICAM -1 correlateds negatively with IL-10 in activity vitiligo.
方法:选择湖南省衡阳地区汉族人群白癜风患者60名和40名健康志愿者为研究对象,采肘静脉血4毫升,取血浆,应用ELISA法检测血浆中sICAM-1及IL-10的浓度。
结果:①白癜风患者血浆中sICAM-1的浓度明显高于健康对照组(P<0.01)进展期与泛发型白癜风患者血浆sICAM-1的浓度分别显著高于稳定期和局限型患者(P<0.01),而稳定期与局限型白癜风患者血浆中sICAM-1的浓度与健康对照组无差异(P>0.05)。②白癜风患者血浆中IL-10的浓度明显低于健康对照组(P<0.01),进展期与泛发型白癜风患者血浆中IL-10的浓度分别低于稳定期与局限型患者(P<0.01,P<0.05),而稳定期与局限型白癜风患者血浆中IL-10的浓度与健康对照组无差异(P>0.05)。③白癜风患者组血浆中的sICAM-1与IL-10的浓度呈负相关(r=-0.37,p=0.042),且进展期及泛发型白癜风患者血浆中sICAM-1上升的浓度与IL-10下降的浓度有明显负相关关系(r=-0.82, p=0.001,r=-0.49,p=0.002)。
结论:1白癜风患者血浆中sICAM-1和IL-10表达异常,表明二者可能参与了白癜风的发病。
2在白癜风病情活动时,sICAM-1与IL-10呈负相关。
Objectives: To explore the relationship between intercellular adhesion molecule-1 and Interieukin-10 among Han people in Hengyang of Hunan province with vitiligo, and to provide the new theory and clinical evidence for vitiligo treatment methods.
Methods: A enzyme-linked immuno sorbent assay was used to detect the levels of s ICAM -1 and IL - 10 in plasma by collecting cubital vein blood of 60 vitiligo patients and 40 normal controls among Han people in Hengyang of Hunan province.
Results:①The plasma level of sICAM -1 in vitiligo is higher than Healthy controls(P<0.01). The level of sICAM -1 in the plasma of patients with progressive and diffuse vitiligo was significantiy higher than the stable and localized vitiligo(P<0.01). No significant difference was obsevent(P > 0. 05) between in sera sICAM -1 level with stable and localized vitiligo and the healthy control.②The level of IL-10 in the plasma of patients with vitiligo was obviously belower than the healthy controls(P<0.01). The level of IL-10 in the plasma of patients with progressive and diffuse vitiligo was obviously belower than the stable and localized vitiligo(P<0.01,P<0.05).No obviously difference was obsevent in sera IL-10 level between patients with stable and localized vitiligo and the healthy control(P > 0. 05).③Negative correlation between sICAM-1and IL-10 was obsevent in progressive and diffuse vitiligo.The changes of sICAM -1 was significantiy associated with The changes of IL-10.
Conclusion: 1 vitiligo is associated with abnormal expression of t sICAM-1and IL-10 in serum, it indicats that they are involved in vitiligo.
2 sICAM -1 correlateds negatively with IL-10 in activity vitiligo.
引文
[1] PY Basak ,AK Adiloglu ,AM Ceyhan, etal. The role of helper and regulatory T cells in the pathogenesis of vitiligo.J Am Acacd Dermatol February 1,2009;60(2):256-60.
[2] A G commodaro,R Belfort Neto ,J P peron,etal. C ytokines Profie rather than T regulator cells correlate with the pathogenesis of vogt-koyanaygi-harada(VKH) syndrome[J]. Ivest ophthalmol vis sci,Apr 2009;50:3583.
[3]Yu HS,Chang KL,Yu CL,et al.Alteration in IL -6,IL -8,GM-CSF,TNF-alpha,and IFN-gamma release by peripheral mononuclear cells in patients with active vilitigo [J].J Invest Dermatol,1997,108:527-529.
[4] Moretti S, Spallanzani A, Amato L, et al .New insights into the pathogenesis of vitiligo:i mbalance of ep idermal cytokines at sites of lesions[J]. Pigment Cell Res, 2002, 15 ( 2 ) : 87- 92.
[5]Hwang JH, Ahn JS ,kIM SD ,et al. The changes of soluble intercellular adhesion molecule -1 after systemic steroid treatment in vitiligo[J ].Dermatol Sci,1999,22:11-1 6.
[6]Das PK, Vanden Wijingaard RM, Wankowicz Kalinska A, et al. A symbiotic concept of autoimmunity and tummor immunity;lessons from vitiligo[J].Trends Inmunol 2001,22:130-136.
[7]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388.
[8]朱文元.白癜风与黄褐斑[M].东南大学出版社,2002:125.
[9]中国中西医结合学会皮肤性病专业委员会色素病学组.白癜风临床分型及疗效标准(2003年修定稿)[J].中华皮肤科杂志,2004,37(7):440.
[10] H Hatanaka,Y Abe,T kamiya, et al.Clinical implications of interleukin-10 induced by non-small-cell lung cancer[J].Ann Onc,Jul,2000,11:815-819.
[11]K Sondergaard,K Stengarrd-pedersen,H Zachariae,et al.Soluble cell adhesion molecules-1 and Soluble Interieukin-2 receptors in scleroderma skin[J].RheumatologyMar 1998,37:304-310.
[12]MR Namaz .novel additions to the dermatologic arsenal [J].?Exp Dermatol,June 1, 2004;13(6): 337-9.
[13] Abdallah M, Abdel - Naser MB, Moussa MH, et al. Sequential i mmunohist ochemical study of dep igmenting andrep igmentingminigrafts in vitiligo [ J ]. Eur J Dermat ol, 2003, 13 (6) : 548– 552.
[14]宋秀祖,许爱娥,周荷芬.白癜风患者调节性T细胞及单核细胞趋化蛋白-1及可溶性细胞间黏附分子-1的检测[J].中华皮肤科杂志,2006,39(2):95-97.
[15]HL Kaufman, S Cohen, K Cheung.Local delivery of vaccinia virus expressing multiple costimulatory molecules for the treatment of established tumors[J]. Hum Gene Ther, February 1, 2006; 17(2): 239-44.
[16]郑凯平刘先洲。卡介菌多糖核酸对白癜风患者血清SIL-2R,IL-10,TNF-R的影响[J].医学临床研究,2005,22(11):1523-1525.
[17] Owen CM, Harris on PV. Successful treat ment of severe psoriasis with basilixi mab, an interleukin- 2 recep tormonoclonal antibody[ J ]. Clin Exp Der matol, 2000, 25 (3) : 195.
[18] A Abanmi,F Al Harthi ,A Zouman,etal. Association of Interleukin-10 gene promoter polymorphisms in Saudi patients with vitiligo[ J ]. Dis Markers January 1,2008 ,24(1):51-7.
[19]陈惠英,许爱娥。白癜风表皮微环境研究进展,中国皮肤性病学杂志2008年2月第22卷第2期:113-115.
[20] SV Skurkovich ,B Skurkovich ,JA Klly .Anticytokine therapy new approach to the treatment of autoimmune and cytokine disturbance diseases. Med Hypotheses,December 1,2002;59(6):770-80.
[21] Zhang LJ , Yu J P , Li D , et al . Effect of cytokines on carbon tet2rachloride2induced hepatic fibrogenesis in rat s [ J ] . World J Gas2 t roenterol , 2004 ,10 (1) :77～81.
[22] Wang XZ , Chen ZX , Zhang LJ , et al . Expression of insulin2like growt h factor 1 and insulin2like growt h factor 1 receptor and it sintervention by interleukin210 inexperimental hepatic fibrosis[J ] .World J Gast roenterol , 2003 ,9 (6) :1287～129.
[23]陈治新,王小众,张莉娟,等. IL-10对实验性肝纤维化大鼠血小板衍生生长因子2AA、BB表达的影响[J ] .临床消化病杂志,2003 ,15 (3) :99～101.
[24]陈治新,王小众,陈运新,等.白介素-10与血小板衍生生长因子对肝星状细胞表达细胞间黏附分子-1 mRNA的影响[J ] .中西医结合肝病杂志,2004 ,14 (1) :16～18.
[25] ZA Taher, G Lauzon, S Maguiness ,etal .Aualysis of Interleukin-10 levels in lesion of vitiligo following treatment with topical tacrolimus.Br J Dermatol September 1 ,2009, 161(3):654-9.
[26]许冰银屑病与白瘫风患者血清中三种细胞因子的检测[J].中华皮肤科杂志2001,34(4):280-281.
[27]汪京峡,段爱霞,张大军白瘫风患者血清中白介素及肿瘤坏死因子水平变化的研究[J]皮肤与性病2006 ;28(1):4-5.
[28]白曼丽,龚少逸.白癜风与血液中相关因子的关系[J]岭南皮肤性病科杂志2007:14(6)380-383.
[29]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388.
[30]陈治新,张莉娟,郑伟达白介素-10对肝纤维化大鼠星状细胞表达细胞间黏附分子-1的影响中国医院药学杂志2005 ,25 (6):490-492.
[31]Kirmbauer R, Charvat B, Schauer E, et al. Modulation of intercellular adhesion molecule-1 expression on human melanocytes and melanoma cells: Evidenc for a regulatory role of IL -6、IL -7、INF -βand UVB light [J].J Invest Dermatol,1992,98:320-32.
[32] Becherel PA , Le G off L , K torza S , et al . Interleukin - 10 inhibits IgE -mediated nitric oxide synthase induction and cytokine synthesis in normal human keratinocytes. Eur J Immunol , 1995 , 25 (10) :2992 - 2995.
[33]PE Grimes ,R Morris ,E Avaniss-Aghajani ,etal. Topical . tacrolimus therapy for vitiligo: therapeutic responses and skin messenger RNA expression of proinflammatory cytokines. J Am Acad Dermatol July 1,2004:51(1):52-61.
[34] Morelli JG ,Norris DA.Influence of infiammatory mediators and cyto-kines on human melgnocyte function . J Invest dermatol, 1993,100(2suppl): S191-195.
[35] G0ff WL,Johnson WC,C1 CW.Babesia boris immunity.In vitor and in vivo evidence for IL-10 regulation of I FN—g a mma and i NOS .Ann N Y Acad Sci. 1998.849:161-180.
[36]尉雁,董琛陈丽瑛.白癜风患者外周血单个核细胞IL-2,IL-10和IFN-γ的表达特点[J].山西医药杂志,2006,8(35):686-688
[37] Escobar A,Lopez M,Serrano A,et al.Dendritic cell immunizations alone or combined with low doses of interleukin-1 induce specifie immune responses in melanoma patients[J],Clin Exp Immunol,2005,142(3):555-568.
[38] Gogas H,Ioannovich J,Dafni U,etal.Prognostic significance of autoimmunity during treatment ofmelanomawith interferon[J].New Engl J Med,2006,354(7):758-760.
[39] De Wanl, Malefyt R,Abrams J .Benett B,Interleukin inhibits cytokine by human monocytes,autoregulatory role of IL-10 produced by monocytes[J] Exp Med,1998,174:1209-1220.
[1]MR Namaz .novel additions to the dermatologic arsenal?[J].Exp Dermatol,June 1, 2004; 13(6): 337-9.
[2]Hwang JH., Ahn JS ,kIM SD ,et al. The changes of soluble intercellular adhesion molecule -1 after systemic steroid treatment in vitiligo[J ].Dermatol Sci,1999 ,22:11-16
[3]Das PK, Vanden Wijingaard RM, Wankowicz Kalinska A, et al. A symbiotic concept of autoimmunity and tummor immunity;lessons from vitiligo[J].Trends Inmunol 2001,22:130-136
[4]宋秀祖,许爱娥,周荷芬.白癜风患者调节性T细胞及单核细胞趋化蛋白-1及可溶性细胞间黏附分子-1的检测[J].中华皮肤科杂志,2006,39(2):95-97
[5]HL Kaufman, S Cohen, K Cheung.Local delivery of vaccinia virus expressing multiple costimulatory molecules for the treatment of established tumors[J]. Hum Gene Ther, February 1, 2006; 17(2): 239-44.
[6]洪为松,尉晓东,周渭衍,等.白癜风患者血清中抗酪氨酸酶抗体及可溶性细胞间黏附分子1的检测[J].中华皮肤科杂志,2005,38(10):612-614
[7]Abdallah M, Abdel Naser MB, Moussa MH, et al. Sequential immunohistochemical study of de pigmenting and vepigmenting minigrafts in vitiligo [J].Br Dermatol,2003 ,148:95-101
[8]顾劲松,徐彩霞,谭雪晶,等.白癜风患者血清白介素,肿瘤坏死因子a及粒细胞-巨细胞集落刺激因子检测[J].中华皮肤科杂志,2003, 36(2):73-75
[9]Yu HS,Chang KL,Yu CL,etal.Alteration in IL-6,IL-8,GM-CSF,TNF-alpha,and IFN-gamma release by peripheral mononuclear cells in patients with active vitiligo[J].J Invest Dermatol,1997,108:527-529
[10]陈建华,曾静,钟文俊.白癜风患者血清及皮肤组织液sICAM-1和IL-12的水平[J].郧阳医学院学报,2008,27(3):224-226
[11]Hedley SJ , Metcalfe R, Gawkrodger DJ ,etal .Vitiligo melanocytes in long-term culture show normal constitutive and cytokine-induce expression of intercellular adhesion molecules-1 and major histocompatibility complex class 1 and 2molecules[J]. Br Dermatol,1998,139:965-973
[12]Lu Y ,Zhu WY,TanC, et al. Melanocytes are potential immunocompetent cells : evidence form recognition of immunological characteristics of cultured human melanocytes[J]. Pigment Cell Res,2002,15:454-460
[13]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388
[14]Moreli JG, Norrris DA. Influence of inflammatory mediators and cytokines on human melanocyte function[J]. J Invest Dermatol 1993;100(2 suppl):191-195
[15]Huang CL, Nordlund JJ, Boissy R. Vitiligo: a manifesta2tion of apop tosis? [ J ]. Am J Clin Dermatol, 2002, 3(5) :301 - 308.
[16]尉雁,董琛陈丽瑛.白癜风患者外周血单个核细胞IL-2,IL-10和IFN-γ的表达特点[J].山西医药杂志,2006,8(35):686-688
[17]Escobar A,Lopez M,Serrano A,et al.Dendritic cell immunizations alone or combined with low doses of interleukin-1 induce specifie immune responses in melanoma patients[J],Clin Exp Immunol,2005,142(3):555-568
[18]Gogas H,Ioannovich J,Dafni U,etal.Prognostic significance of autoimmunity during treatment of melanoma with interferon[J].New Engl J Med,2006,354(7):758-760
[19]魏玉平,白瑛,陈雪红,等.白癜风患者外周血IL-2 IL-4 TNF-a的测定及临床意义[J].中国麻风皮肤病杂志, 2004, 20(1):43-44
[20]曾静,张启国,方春红,等.姚莹肿瘤坏死因子-α及其受体在白癜风患者皮损中的表达[J],中国皮肤性病学杂志,2006,20(7):409-410
[2] A G commodaro,R Belfort Neto ,J P peron,etal. C ytokines Profie rather than T regulator cells correlate with the pathogenesis of vogt-koyanaygi-harada(VKH) syndrome[J]. Ivest ophthalmol vis sci,Apr 2009;50:3583.
[3]Yu HS,Chang KL,Yu CL,et al.Alteration in IL -6,IL -8,GM-CSF,TNF-alpha,and IFN-gamma release by peripheral mononuclear cells in patients with active vilitigo [J].J Invest Dermatol,1997,108:527-529.
[4] Moretti S, Spallanzani A, Amato L, et al .New insights into the pathogenesis of vitiligo:i mbalance of ep idermal cytokines at sites of lesions[J]. Pigment Cell Res, 2002, 15 ( 2 ) : 87- 92.
[5]Hwang JH, Ahn JS ,kIM SD ,et al. The changes of soluble intercellular adhesion molecule -1 after systemic steroid treatment in vitiligo[J ].Dermatol Sci,1999,22:11-1 6.
[6]Das PK, Vanden Wijingaard RM, Wankowicz Kalinska A, et al. A symbiotic concept of autoimmunity and tummor immunity;lessons from vitiligo[J].Trends Inmunol 2001,22:130-136.
[7]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388.
[8]朱文元.白癜风与黄褐斑[M].东南大学出版社,2002:125.
[9]中国中西医结合学会皮肤性病专业委员会色素病学组.白癜风临床分型及疗效标准(2003年修定稿)[J].中华皮肤科杂志,2004,37(7):440.
[10] H Hatanaka,Y Abe,T kamiya, et al.Clinical implications of interleukin-10 induced by non-small-cell lung cancer[J].Ann Onc,Jul,2000,11:815-819.
[11]K Sondergaard,K Stengarrd-pedersen,H Zachariae,et al.Soluble cell adhesion molecules-1 and Soluble Interieukin-2 receptors in scleroderma skin[J].RheumatologyMar 1998,37:304-310.
[12]MR Namaz .novel additions to the dermatologic arsenal [J].?Exp Dermatol,June 1, 2004;13(6): 337-9.
[13] Abdallah M, Abdel - Naser MB, Moussa MH, et al. Sequential i mmunohist ochemical study of dep igmenting andrep igmentingminigrafts in vitiligo [ J ]. Eur J Dermat ol, 2003, 13 (6) : 548– 552.
[14]宋秀祖,许爱娥,周荷芬.白癜风患者调节性T细胞及单核细胞趋化蛋白-1及可溶性细胞间黏附分子-1的检测[J].中华皮肤科杂志,2006,39(2):95-97.
[15]HL Kaufman, S Cohen, K Cheung.Local delivery of vaccinia virus expressing multiple costimulatory molecules for the treatment of established tumors[J]. Hum Gene Ther, February 1, 2006; 17(2): 239-44.
[16]郑凯平刘先洲。卡介菌多糖核酸对白癜风患者血清SIL-2R,IL-10,TNF-R的影响[J].医学临床研究,2005,22(11):1523-1525.
[17] Owen CM, Harris on PV. Successful treat ment of severe psoriasis with basilixi mab, an interleukin- 2 recep tormonoclonal antibody[ J ]. Clin Exp Der matol, 2000, 25 (3) : 195.
[18] A Abanmi,F Al Harthi ,A Zouman,etal. Association of Interleukin-10 gene promoter polymorphisms in Saudi patients with vitiligo[ J ]. Dis Markers January 1,2008 ,24(1):51-7.
[19]陈惠英,许爱娥。白癜风表皮微环境研究进展,中国皮肤性病学杂志2008年2月第22卷第2期:113-115.
[20] SV Skurkovich ,B Skurkovich ,JA Klly .Anticytokine therapy new approach to the treatment of autoimmune and cytokine disturbance diseases. Med Hypotheses,December 1,2002;59(6):770-80.
[21] Zhang LJ , Yu J P , Li D , et al . Effect of cytokines on carbon tet2rachloride2induced hepatic fibrogenesis in rat s [ J ] . World J Gas2 t roenterol , 2004 ,10 (1) :77～81.
[22] Wang XZ , Chen ZX , Zhang LJ , et al . Expression of insulin2like growt h factor 1 and insulin2like growt h factor 1 receptor and it sintervention by interleukin210 inexperimental hepatic fibrosis[J ] .World J Gast roenterol , 2003 ,9 (6) :1287～129.
[23]陈治新,王小众,张莉娟,等. IL-10对实验性肝纤维化大鼠血小板衍生生长因子2AA、BB表达的影响[J ] .临床消化病杂志,2003 ,15 (3) :99～101.
[24]陈治新,王小众,陈运新,等.白介素-10与血小板衍生生长因子对肝星状细胞表达细胞间黏附分子-1 mRNA的影响[J ] .中西医结合肝病杂志,2004 ,14 (1) :16～18.
[25] ZA Taher, G Lauzon, S Maguiness ,etal .Aualysis of Interleukin-10 levels in lesion of vitiligo following treatment with topical tacrolimus.Br J Dermatol September 1 ,2009, 161(3):654-9.
[26]许冰银屑病与白瘫风患者血清中三种细胞因子的检测[J].中华皮肤科杂志2001,34(4):280-281.
[27]汪京峡,段爱霞,张大军白瘫风患者血清中白介素及肿瘤坏死因子水平变化的研究[J]皮肤与性病2006 ;28(1):4-5.
[28]白曼丽,龚少逸.白癜风与血液中相关因子的关系[J]岭南皮肤性病科杂志2007:14(6)380-383.
[29]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388.
[30]陈治新,张莉娟,郑伟达白介素-10对肝纤维化大鼠星状细胞表达细胞间黏附分子-1的影响中国医院药学杂志2005 ,25 (6):490-492.
[31]Kirmbauer R, Charvat B, Schauer E, et al. Modulation of intercellular adhesion molecule-1 expression on human melanocytes and melanoma cells: Evidenc for a regulatory role of IL -6、IL -7、INF -βand UVB light [J].J Invest Dermatol,1992,98:320-32.
[32] Becherel PA , Le G off L , K torza S , et al . Interleukin - 10 inhibits IgE -mediated nitric oxide synthase induction and cytokine synthesis in normal human keratinocytes. Eur J Immunol , 1995 , 25 (10) :2992 - 2995.
[33]PE Grimes ,R Morris ,E Avaniss-Aghajani ,etal. Topical . tacrolimus therapy for vitiligo: therapeutic responses and skin messenger RNA expression of proinflammatory cytokines. J Am Acad Dermatol July 1,2004:51(1):52-61.
[34] Morelli JG ,Norris DA.Influence of infiammatory mediators and cyto-kines on human melgnocyte function . J Invest dermatol, 1993,100(2suppl): S191-195.
[35] G0ff WL,Johnson WC,C1 CW.Babesia boris immunity.In vitor and in vivo evidence for IL-10 regulation of I FN—g a mma and i NOS .Ann N Y Acad Sci. 1998.849:161-180.
[36]尉雁,董琛陈丽瑛.白癜风患者外周血单个核细胞IL-2,IL-10和IFN-γ的表达特点[J].山西医药杂志,2006,8(35):686-688
[37] Escobar A,Lopez M,Serrano A,et al.Dendritic cell immunizations alone or combined with low doses of interleukin-1 induce specifie immune responses in melanoma patients[J],Clin Exp Immunol,2005,142(3):555-568.
[38] Gogas H,Ioannovich J,Dafni U,etal.Prognostic significance of autoimmunity during treatment ofmelanomawith interferon[J].New Engl J Med,2006,354(7):758-760.
[39] De Wanl, Malefyt R,Abrams J .Benett B,Interleukin inhibits cytokine by human monocytes,autoregulatory role of IL-10 produced by monocytes[J] Exp Med,1998,174:1209-1220.
[1]MR Namaz .novel additions to the dermatologic arsenal?[J].Exp Dermatol,June 1, 2004; 13(6): 337-9.
[2]Hwang JH., Ahn JS ,kIM SD ,et al. The changes of soluble intercellular adhesion molecule -1 after systemic steroid treatment in vitiligo[J ].Dermatol Sci,1999 ,22:11-16
[3]Das PK, Vanden Wijingaard RM, Wankowicz Kalinska A, et al. A symbiotic concept of autoimmunity and tummor immunity;lessons from vitiligo[J].Trends Inmunol 2001,22:130-136
[4]宋秀祖,许爱娥,周荷芬.白癜风患者调节性T细胞及单核细胞趋化蛋白-1及可溶性细胞间黏附分子-1的检测[J].中华皮肤科杂志,2006,39(2):95-97
[5]HL Kaufman, S Cohen, K Cheung.Local delivery of vaccinia virus expressing multiple costimulatory molecules for the treatment of established tumors[J]. Hum Gene Ther, February 1, 2006; 17(2): 239-44.
[6]洪为松,尉晓东,周渭衍,等.白癜风患者血清中抗酪氨酸酶抗体及可溶性细胞间黏附分子1的检测[J].中华皮肤科杂志,2005,38(10):612-614
[7]Abdallah M, Abdel Naser MB, Moussa MH, et al. Sequential immunohistochemical study of de pigmenting and vepigmenting minigrafts in vitiligo [J].Br Dermatol,2003 ,148:95-101
[8]顾劲松,徐彩霞,谭雪晶,等.白癜风患者血清白介素,肿瘤坏死因子a及粒细胞-巨细胞集落刺激因子检测[J].中华皮肤科杂志,2003, 36(2):73-75
[9]Yu HS,Chang KL,Yu CL,etal.Alteration in IL-6,IL-8,GM-CSF,TNF-alpha,and IFN-gamma release by peripheral mononuclear cells in patients with active vitiligo[J].J Invest Dermatol,1997,108:527-529
[10]陈建华,曾静,钟文俊.白癜风患者血清及皮肤组织液sICAM-1和IL-12的水平[J].郧阳医学院学报,2008,27(3):224-226
[11]Hedley SJ , Metcalfe R, Gawkrodger DJ ,etal .Vitiligo melanocytes in long-term culture show normal constitutive and cytokine-induce expression of intercellular adhesion molecules-1 and major histocompatibility complex class 1 and 2molecules[J]. Br Dermatol,1998,139:965-973
[12]Lu Y ,Zhu WY,TanC, et al. Melanocytes are potential immunocompetent cells : evidence form recognition of immunological characteristics of cultured human melanocytes[J]. Pigment Cell Res,2002,15:454-460
[13]隋连金,戎丽娟,王炜.白癜风患者血清相关因子的检测[J].中国麻风皮肤病杂志, 2006,22(5):386-388
[14]Moreli JG, Norrris DA. Influence of inflammatory mediators and cytokines on human melanocyte function[J]. J Invest Dermatol 1993;100(2 suppl):191-195
[15]Huang CL, Nordlund JJ, Boissy R. Vitiligo: a manifesta2tion of apop tosis? [ J ]. Am J Clin Dermatol, 2002, 3(5) :301 - 308.
[16]尉雁,董琛陈丽瑛.白癜风患者外周血单个核细胞IL-2,IL-10和IFN-γ的表达特点[J].山西医药杂志,2006,8(35):686-688
[17]Escobar A,Lopez M,Serrano A,et al.Dendritic cell immunizations alone or combined with low doses of interleukin-1 induce specifie immune responses in melanoma patients[J],Clin Exp Immunol,2005,142(3):555-568
[18]Gogas H,Ioannovich J,Dafni U,etal.Prognostic significance of autoimmunity during treatment of melanoma with interferon[J].New Engl J Med,2006,354(7):758-760
[19]魏玉平,白瑛,陈雪红,等.白癜风患者外周血IL-2 IL-4 TNF-a的测定及临床意义[J].中国麻风皮肤病杂志, 2004, 20(1):43-44
[20]曾静,张启国,方春红,等.姚莹肿瘤坏死因子-α及其受体在白癜风患者皮损中的表达[J],中国皮肤性病学杂志,2006,20(7):409-410