水稻OsBIANK1和亚硫酸盐氧化酶基因的功能分析
|
摘要
锚蛋白(ANK)是生命体中一类庞大的类群,研究表明一些锚蛋白参与植物防卫反应的调控。本实验室前期研究发现水稻锚蛋白基因OsBIANKl在抗病反应中上调表达。为了研究OsBIANK1在抗病反应中的功能,构建了过量表达载体35S：：OsBIANK1,并利用根癌农杆菌介导的花序浸蘸法导入拟南芥中,获得35S：：OsBIANK1单拷贝株系,转代选取3个纯合株系(OsBIANK1-OE#17、#24和#29)。比较了野生型、OsBIANK1-OE植株对Pseudomonas syringae pv. tomato (Pst)DC3000和灰霉病菌(Botrytis cinerea)的抗病性变化。注射接种Pst DC3000、活体接种B. cinerea后,OsBIANK1-OE植株上病害显著轻于野生型。Pst DC3000侵染后,OsBIANK1-OE的Pst DC3000菌量显著低于野生型对照。OsBIANK1-OE#17、#24和#29植株中PR-1和PR-5基因表达上调；而B. cinerea侵染后,OsBIANK1-OE植株中PDF1.2和BIK1基因表达上调。此外,经水杨酸(salicylic acid, SA)和苯并噻二唑(benzothiadiazole, BTH)诱导2天后,再接种PstDC3000, OsBIANK1转基因拟南芥植株菌量低于野生型对照,且低于没有经SA和BTH诱导后的植株。在B. cinerea侵染后,OsBIANK1-OE植株叶片中仅有少量活性氧积累。这些结果表明,OsBIANK1在拟南芥中对不同类型病害的抗病反应中起正调控作用。
亚硫酸盐氧化酶(sulfite oxidase, SO)是近几年在植物中得到证实的一个钼酶,在植物硫代谢中起着重要的作用,具有亚硫酸盐解毒功能。近期研究,都集中在SO基因的生化特性方面,抗病性方面报道至今未见报道。本研究中,我们分离鉴定了水稻中两个SO酶基因OsSO1和OsSO2。为了研究OsSO1和OsSO2在抗病反应中的功能,构建了过量表达载体35S::OsSO1,35S::OsSO2,并利用根癌农杆菌介导的花序浸蘸法导入拟南芥中,获得35S::OsSO1和35S::OsSO2单拷贝株系,转代选取纯合株系(OsSO1-OE#19、#23和OsSO2-OE#4)。比较了野生型、OsSO1-OE、OsSO2-OE植株对Pst DC3000和B. cinerea的抗病性变化。注射接种Pst DC300、活体接种B. cinerea后，OsSO1-OE和OsSO2-OE植株上病害显著轻于野生型。Pst DC300侵染后,OsSO1-OE和OsSO2-OE的Pst DC3000菌量显著低于野生型对照,OsSO1-OE和OsSO2-OE植株中PR-1和PR-5基因表达上调；而活体接种B. cinerea后,OsSO1-OE和OsSO2-OE植株中PDF1.2和BIK1基因表达上调。此外,经SA和BTH诱导2天后,再接种Pst DC3000, OsSO1和OsSO2转基因拟南芥植株菌量低于野生型对照且低于没有经SA和BTH诱导后的植株。在B. cinerea侵染后,OsSO1-OE和OsSO2-OE植株叶片中仅有少量活性氧积累。这些结果表明,OsSO1和OsSO2在拟南芥中对不同类型病害的抗病反应中起正调控作用,而且可能通过SA信号途径。
Ankyrin repeat-containing proteins are abundant protein classes functioning in protein-protein interactions. Ankyrin repeat proteins (ANK) have been reported to be involved in various biological processes of plant growth and development, transport and defense responses. Previously, we identified a gene, OsBIANK1, encoding for an ANK protein, was up-regulated in defense response in rice after infection by Magnaporthe grisea or treatment with benzothiadiazole (BTH). To study the function of OsBIANK1 in disease resistance response, I constructed an overexpression vector 35S::OsBIANK1, and introduced into wild-type Arabidopsis plants using floral dip transformation protocol.35S::OsBIANK1 transgenic lines with single copy of the transgene were obtained. Homozygous lines were identified from the 3rd generation through screening and three homozygous lines for 35S::OsBIANK1 (OsBIANK1-OE#17,#24and #29) were chosen for further work. Disease phenotypes of the wild-type, OsBIANK1-OE plants were compared after inoculation with Pseudomonas syringae pv. tomato (Pst) DC3000 and Botrytis cinerea. As compared with the wild-type plants, the OsBIANK1-OE plants showed a significant reduced susceptibility to the pathogens. Furthermore, growth of Pst DC3000 in leaves of the OsBIANK1-OE plants was significantly reduced. After inoculation with Pst DC3000, the expression of PR-1 and PR-5 genes was up-regulated in the OsBIANK1-OE plants. And after inoculation with B. cinerea, expression of PDF1.2 and BIK1 genes was up-regulated in the OsBIANK1-OE plants. Additionally, after two days of treatment with some well-known defense-related chemical inducers such as salicylic acid (SA) and benzothaidiazole (BTH), the OsBIANK1-OE plants also showed a significant reduced susceptibility to Pst DC3000, growth of Pst DC3000 in leaves of the OsBIANK1-OE plants was also significantly reduced and less than that without treatment with SA and BTH. Moreover, after Botrytis infection, accumulation of reactive oxygen species (H2O2 and superoxide anion) in leaves of the OsBIANK1-OE plants was lower than those of the wild type plants. These results indicate that OsBIANK1 is a positive regulator of defense responses in Arabidopsis thaliana against both necrotrophic and biotrophic pathogens.
Sulfite oxidase (SO) is one of molybdenum(Mo) enzymes in plant. SO has been reported to be involved in sulphur metabolism in plants, and can detoxifies excessive sulfite. Information on functions of SO in plant defense response against pathogen infection is lacking. In this study, I identified two SO genes from rice. To study the function of OsSO1 and OsSO2 in disease resistance response, I constructed overexpression vectors 35S::OsSO1 and 35S::OsSO2 and introduced them into wild-type plants using floral dip transformation protocol.35S::OsSO1 and 35S::OsSO2 transgenic lines with single copy of the transgene were obtained. Homozygous lines were identified from the 3rd generation through screening and three homozygous lines for 35S::OsSO1 (OsSO1-OE#19,#23) and 35S::OsSO2(OsSO2-OE#4) were chosen for further work. Disease phenotypes of the wild-type, OsSO1-OE and OsSO2-OE plants were compared after inoculation with Pst DC3000 and B. cinerea. As compared with the wild-type plants, the OsSO1-OE and OsSO2-OE plants showed a significant reduced susceptibility to the patogens. Furthermore, growth of Pst DC3000 in leaves of the OsSO1-OE and OsSO2-OE plants was significantly reduced. After inoculation with Pst DC3000, the expression of PR-1 and PR-5 genes was up-regulated in the OsSO1-OE and OsSO2-OE plants. And after inoculation with B. cinerea, expression of PDF1.2 and BIK1 genes was up-regulated in the OsSO1-OE and OsSO2-OE plants. Additionally, after two days of treatment with SA and BTH, the OsSO1-OE and OsSO2-OE plants showed a significant reduced susceptibility to Pst DC3000, growth of Pst DC3000 in leaves of the OsSO1-OE and OsSO2-OE plants was also significantly reduced and less than that without treatment with SA and BTH. Moreover, accumulation of reactive oxygen species in leaves of the OsSO1-OE and OsSO2-OE plants after Botrytis infection was less than that in the wild type plants. These results indicate that OsSO1 and OsSO2 are involved in defense response against pathogen infection.
亚硫酸盐氧化酶(sulfite oxidase, SO)是近几年在植物中得到证实的一个钼酶,在植物硫代谢中起着重要的作用,具有亚硫酸盐解毒功能。近期研究,都集中在SO基因的生化特性方面,抗病性方面报道至今未见报道。本研究中,我们分离鉴定了水稻中两个SO酶基因OsSO1和OsSO2。为了研究OsSO1和OsSO2在抗病反应中的功能,构建了过量表达载体35S::OsSO1,35S::OsSO2,并利用根癌农杆菌介导的花序浸蘸法导入拟南芥中,获得35S::OsSO1和35S::OsSO2单拷贝株系,转代选取纯合株系(OsSO1-OE#19、#23和OsSO2-OE#4)。比较了野生型、OsSO1-OE、OsSO2-OE植株对Pst DC3000和B. cinerea的抗病性变化。注射接种Pst DC300、活体接种B. cinerea后，OsSO1-OE和OsSO2-OE植株上病害显著轻于野生型。Pst DC300侵染后,OsSO1-OE和OsSO2-OE的Pst DC3000菌量显著低于野生型对照,OsSO1-OE和OsSO2-OE植株中PR-1和PR-5基因表达上调；而活体接种B. cinerea后,OsSO1-OE和OsSO2-OE植株中PDF1.2和BIK1基因表达上调。此外,经SA和BTH诱导2天后,再接种Pst DC3000, OsSO1和OsSO2转基因拟南芥植株菌量低于野生型对照且低于没有经SA和BTH诱导后的植株。在B. cinerea侵染后,OsSO1-OE和OsSO2-OE植株叶片中仅有少量活性氧积累。这些结果表明,OsSO1和OsSO2在拟南芥中对不同类型病害的抗病反应中起正调控作用,而且可能通过SA信号途径。
Ankyrin repeat-containing proteins are abundant protein classes functioning in protein-protein interactions. Ankyrin repeat proteins (ANK) have been reported to be involved in various biological processes of plant growth and development, transport and defense responses. Previously, we identified a gene, OsBIANK1, encoding for an ANK protein, was up-regulated in defense response in rice after infection by Magnaporthe grisea or treatment with benzothiadiazole (BTH). To study the function of OsBIANK1 in disease resistance response, I constructed an overexpression vector 35S::OsBIANK1, and introduced into wild-type Arabidopsis plants using floral dip transformation protocol.35S::OsBIANK1 transgenic lines with single copy of the transgene were obtained. Homozygous lines were identified from the 3rd generation through screening and three homozygous lines for 35S::OsBIANK1 (OsBIANK1-OE#17,#24and #29) were chosen for further work. Disease phenotypes of the wild-type, OsBIANK1-OE plants were compared after inoculation with Pseudomonas syringae pv. tomato (Pst) DC3000 and Botrytis cinerea. As compared with the wild-type plants, the OsBIANK1-OE plants showed a significant reduced susceptibility to the pathogens. Furthermore, growth of Pst DC3000 in leaves of the OsBIANK1-OE plants was significantly reduced. After inoculation with Pst DC3000, the expression of PR-1 and PR-5 genes was up-regulated in the OsBIANK1-OE plants. And after inoculation with B. cinerea, expression of PDF1.2 and BIK1 genes was up-regulated in the OsBIANK1-OE plants. Additionally, after two days of treatment with some well-known defense-related chemical inducers such as salicylic acid (SA) and benzothaidiazole (BTH), the OsBIANK1-OE plants also showed a significant reduced susceptibility to Pst DC3000, growth of Pst DC3000 in leaves of the OsBIANK1-OE plants was also significantly reduced and less than that without treatment with SA and BTH. Moreover, after Botrytis infection, accumulation of reactive oxygen species (H2O2 and superoxide anion) in leaves of the OsBIANK1-OE plants was lower than those of the wild type plants. These results indicate that OsBIANK1 is a positive regulator of defense responses in Arabidopsis thaliana against both necrotrophic and biotrophic pathogens.
Sulfite oxidase (SO) is one of molybdenum(Mo) enzymes in plant. SO has been reported to be involved in sulphur metabolism in plants, and can detoxifies excessive sulfite. Information on functions of SO in plant defense response against pathogen infection is lacking. In this study, I identified two SO genes from rice. To study the function of OsSO1 and OsSO2 in disease resistance response, I constructed overexpression vectors 35S::OsSO1 and 35S::OsSO2 and introduced them into wild-type plants using floral dip transformation protocol.35S::OsSO1 and 35S::OsSO2 transgenic lines with single copy of the transgene were obtained. Homozygous lines were identified from the 3rd generation through screening and three homozygous lines for 35S::OsSO1 (OsSO1-OE#19,#23) and 35S::OsSO2(OsSO2-OE#4) were chosen for further work. Disease phenotypes of the wild-type, OsSO1-OE and OsSO2-OE plants were compared after inoculation with Pst DC3000 and B. cinerea. As compared with the wild-type plants, the OsSO1-OE and OsSO2-OE plants showed a significant reduced susceptibility to the patogens. Furthermore, growth of Pst DC3000 in leaves of the OsSO1-OE and OsSO2-OE plants was significantly reduced. After inoculation with Pst DC3000, the expression of PR-1 and PR-5 genes was up-regulated in the OsSO1-OE and OsSO2-OE plants. And after inoculation with B. cinerea, expression of PDF1.2 and BIK1 genes was up-regulated in the OsSO1-OE and OsSO2-OE plants. Additionally, after two days of treatment with SA and BTH, the OsSO1-OE and OsSO2-OE plants showed a significant reduced susceptibility to Pst DC3000, growth of Pst DC3000 in leaves of the OsSO1-OE and OsSO2-OE plants was also significantly reduced and less than that without treatment with SA and BTH. Moreover, accumulation of reactive oxygen species in leaves of the OsSO1-OE and OsSO2-OE plants after Botrytis infection was less than that in the wild type plants. These results indicate that OsSO1 and OsSO2 are involved in defense response against pathogen infection.
引文
AbuQamar S, Chen X, Dhawan R, Bluhm B, Salmeron J, Lam S, Dietrich RA, Mengiste T. (2006) Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J,48:28-44.
Ahmad A, Sarfraz A. (2010) Screening and partial immunochemical characterization of sulfite oxidase from plant source. Indian JExp Biol,48:83-86.
Albert S, Despres B, Guilleminot J, Bechtold N, Pelletier G, Delseny M, Devic M. (1999) The EMB 506 gene encodes a novel ankyrin repeat containing protein that is essential for the normal development of Arabidopsis embryos. Plant J,17:169-179.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. (1997) Gapped BLAST and PSI-BLAST:a new generation of protein database search programs. Nucleic Acids Res,25:3389-3402.
Andrade MA, Perez Iratxeta C, Ponting CP. (2001) Protein repeats:Structures, functions, and evolution. Struct Biol,134:117-131.
Astashkin AV, Johnson Winters K, Klein EL, Byrne RS, Hille R, Raitsimring AM, Enemark JH. (2007) Direct demonstration of the presence of coordinated sulfate in the reaction pathway of Arabidopsis thaliana sulfite oxidase using 33S labeling and ESEEM spectroscopy. J Am Chem Soc,129:14800-14810.
Attaran E, Zeier TE, Griebel T, Zeier J. (2009) Systemic acquired resistance in Arabidopsis is independent of methyl salicylate production and jasmonate signaling. Plant Cell,21:954-971.
Bae W, Lee YJ, Kim DH, Lee J, Kim S, Sohn EJ, Hwang I. (2008) AKR2A-mediated import of chloroplast outer membrane proteinsis essential for chloroplast biogenesis. Nat Cell Biol, 10:220-227.
Batchelor AH, Piper DE, dela Brousse FC, McKnight SL, Wolberger C. (1998) The structure of GABPalpha/beta:an ETS domain- ankyrin repeat heterodimer bound to DNA. Science, 13:1037-1041.
Breeden L, Nasmyth K. (1987) Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila. Nature,329:651-654.
Brychkova G, Xia Z, Yang G, Yesbergenoval Z, Zhang Z, Davydov O, Fluhr R, Sagi M1. (2007) Sulfite Oxidase protects plants against sulfur dioxide t oxicity. Plant J,50:696-709.
Byeon IJ, Li J, Ericson K, Selby TL, Tevelev A, Kim HJ, O'Maille P, Tsai MD. (1998)Tumor suppressor p16INK4A:Determination of solution structure and analyses of its interaction with cyclin-dependent kinase 4. Mol Cell,1:421-431.
Cao H, Glazebrook J, Clarke JD, Volko S, Dong X. (1997) The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell, 88:57-63.
Chern M, Fitzgerald HA, Canlas PE, Navarre DA, Ronald PC. (2005) Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact,18:511-520.
Choi BY, Choi HS, Ko K, Cho YY, Zhu F, Kang BS, Ermakova SP, Ma WY, Bode AM, Dong Z. (2005) The tumor suppressor p16(INK4a) prevents cell transformation through inhibition of c-Jun phosphorylation and AP-1 activity. Nat Struct Mol Biol,12:699-707.
Choi MS, Kim MC, Yoo JH, Moon BC, Koo SC, Park BO, Lee JH, Koo YD, Han HJ, Lee SY, Chung WS, Lim CO, Cho MJ. (2005) Isolation of a calmodulin-binding transcription factor from rice(Oryza sativa L. J Biol Chem,280:40820-40831.
Clough SJ, Bent AF. (1998) Floral dip:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J,16:735-743.
Cohen HJ, Betcher Lange S, Kessler DL, Rajagopalan KV. (1972) Hepatic sulfite oxidase. Congruency in mitochondria of prosthetic groups and activity. J Biol Chem,247:7759-7766.
Coleman KG, Wautlet BS, Morrissey D, Mulheron J, Sedman SA, Brinkley P, Price S, Webster KR. (1997) Identification of CDK4 sequences involved in cyclin D1 and p16 binding. Biol Chem,272:18869-18874.
Cui J, Bahrami AK, Pringle EG, Hernandez Guzman G, Bender CL, Pierce NE, Ausubel FM. (2005) Pseudomonas syringae manipulates systemic plant defenses against pathogens and herbivores. Proc Nat Acad Sci USA,102:1791-1796.
Dai X, Hayashi K, NozakiH, Cheng Y, Zhao Y. (2005) Genetic and chemical analyses of the actionmechanisms of sirtinol in Arabidopsis. Proc Natl Acad Sci USA,102:3129-3134.
Dawson S, Apcher S, Mee M, Higashitsuji H, Baker R, Uhle S, Dubiel W, Fujita J, Mayer RJ. (2002) Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome. Biol Chem,277:10893-10902.
De Vos M, Van Oosten VR, Van Poecke RMP, Van Pelt JA, Pozo MJ, Mueller MJ, Buchala AJ, Metraux JP, Van Loon LC, Dicke M. (2005) Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol Plant Microbe Interact,18:923-937.
Dong X. (2004) NPR1, all things considered. Curr Opin Plant Biol,7:547-552.
Durrant WE, Dong X. (2004) Systemic acquired resistance; Annu Rev Phytopathol,42:185-209.
Eilers T, Schwarz G, Brinkmann H, Witt C, Richter T, Nieder J, Koch B, Hille R, Hansch R, Mendel RR. (2001) Identification and biochemical characterization of Arabidopsis thaliana sulfite bxidase. A new player in plant sulfur metabolism. J Biol Chem,276:46989-46994.
Fitzgerald HA, Chern MS, Navarre R, Ronald PC. (2004) Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype. Mol Plant Microbe Interact,17:140-151.
Foord R, Taylor IA, Sedgwick SG, Smerdon SJ. (1999) X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function. Nat Struct Biol,6:157-165.
Forrer P, Binz HK, Stumpp MT, Pluckthun A. (2004) Consensus design of repeat proteins. Chem BioChem,5:183-189.
Forrer P, Stumpp MT, Binz HK, Pluckthun A. (2003) A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett,539:2-6.
Garcion C, Guilleminot J, Kroj T, Parcy F, Giraudat J, Devic M. (2006) AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis. Plant J,48:895-906.
Gohre V, Robatzek S. (2008) Breaking the barriers:microbial effector molecules subvert plant immunity. Annu Rev Phytopathol,46:189-215.
Gunter Schwarz, Ralf R, Mendel. (2006) Molybdenum Cofactor Biosynthesis and Molybdenum Enzymes. Annu Rev Plant Biol,57:623-647.
Ha CM, Jun JH, Nam HG, Fletcher JC. (2004) BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana. Plant Cell Physiol, 45:1361-1370.
Haenzelmann P, Hernandez HL, Menzel C, Garcia-Serres R, Huynh BH. (2004) Characterization of MOCS1A, an oxygen-sensitive iron-sulfur protein involved in human molybdenum cofactor biosynthesis. J Biol Chem,279:34721-34732.
Hansch R, Lang C, Rennenberg H, Mendel RR. (2007) Significance of plant sulfite oxidase. Plant Biol,9:589-595.
Heber U, H"uve K. (1998) Action of SO2 on plants and metabolic detoxification of SO2. Intl Rev Cytol,177:255-286.
Hemsley PA, Kemp AC, Grierson CS. (2005) The TIP GROWTH DEFECTIVE1 S-acyl transferase regulates plant cell growth in Arabidopsis. Plant Cell,17:2554-2563.
Huang J, Chen F, Del Casino C, Autino A, Shen M, Yuan S, Peng J, Shi H, Wang C, Cresti M, Li Y. (2006) An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily. Plant Physiol,140:1374-1383.
Huxford T, Huang DB, Malek S, Ghosh G.(1998) The crystal structure of the IkBa/NF-kB complex reveals mechanisms of NF-kB inactivation. Cell,95:759-770.
Jianyan Huang, Xiaobo Zhao, Huihui Yu, Yidan Ouyang, Lei Wang, Qifa Zhang. (2009) The ankyrin repeat gene family in rice:genome-wide identification, classification and expression profiling. Plant Mol Biol,71:207-226.
Johnson JL, Rajagopalan KV. (1979) The oxidation of sulphite in animals systems. Ciba Found Symp,72:119-133.
Junan Li, Anjali Mahajan, Ming Daw Tsai. (2006) Ankyrin Repeat:A Unique Motif Mediating Protein-Protein Interactions. Biochemistry,45:15168-15178.
Katagirl F, Thilmony, He SY. (2002) The Arabidopsis Thaliana-Pseudomonas Syringae Interaction. The Arabidopsis Book, doi:10.1199/tab.0039.
Kim MG, Kim SY, Kim WY, Mackey D, Lee SY. (2008) Responses of Arabidopsis thaliana to challenge by Pseudomonas syringae. Mol Cells,25:323-331.
Klimyuk VI, Persello Cartieaux F, Havaux M, Contard David P, Schuenemann D, Meiherhoff K, Gouet P, Jones JD, Hoffman NE, Nussaume L. (1999) A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell,11:87-99.
Kohl A, Binz HK, Forrer P, Stumpp MT, Pluckthun A, Grutter MG (2003) Designed to be stable:Crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci USA,100; 1700-1705.
Koizumi K, Naramoto S, Sawa S, Yahara N, Ueda T, Nakano A, Sugiyama M, Fukuda H. (2005) VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation. Development,132:1699-1711.
Lapointe J, Lachance Y, Labrie Y, Labrie C. (1996) A p18 mutant defective in CDK6 binding in human breast cancer cells. Cancer Res,56:4586-4589.
Leon Reyes A, Spoel SH, De Lange ES, Abe H, Kobayashi M, Tsuda S, Millenaar FF, Welschen RA, Ritsema T, Pieterse CM. (2009) Ethylene modulates the role of NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 in cross talk between salicylate and jasmonate signaling. Plant Physiol,149:1797-1809.
Leustek T, Saito K. (1999) Sulfate transport and assimilation in plants. Plant Physiol, 120:637-644.
Li HY, Chye ML. (2004) Arabidopsis Acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats. Plant Mol Biol, 54:233-243.
Li J, Joo SH, Tsai MD. (2003) An NF-kB-specific inhibitor, IkBa, binds to and inhibits cyclin-dependent kinase 4. Biochemistry,42:13476-13483.
Li J, Poi MJ, Qin D, Selby TL, Byeon IJ, Tsai MD. (2000) Tumor suppressor INK4:Quantitative structure-function analyses of p18INK4C as an inhibitor of cyclin-dependent kinase 4. Biochemistry,39:649-657.
Li J, Tsai MD. (2002) Novel insights into the INK4-CDK4/6-Rb pathway:Counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb. Biochemistry,41:3977-3983.
Li X, Zhang Y, Clarke DJ, Li Y, Dong X. (1999) Identification and cloning of a negative regulator of systemic acquired resistance, SNI1, through a screen for suppressors of nprl-1. Cell,98: 329-339.
Loar JW, Seiser RM, Sundberg AE, Sagerson HJ, Ilias N, Zobel-Thropp P, Craig EA, Lycan DE. (2004) Genetic and biochemical interactions among Yarl, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cere isiae. Genetics,168:1877-1889.
Lux SE, John KM, Bennett V. (1990) Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins. Nature,344:36-42.
Lycan DE, Stafford KA, Bollinger W, Breeden LL. (1996) A new Saccharomyces cere isiae ankyrin repeat-encoding gene required for a normal rate of cell proliferation. Gene,171:33-40.
MacKintosh C, Meek SE. (2001) Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis. Cell Mol Life Sci,58:205-214.
Main ER, Jackson SE, Regan L. (2003) The folding and design of repeat proteins:Reaching a consensus. Curr Opin Struct Biol,13:482-489.
Marcotte EM, Pellegrini M, Yeates TO, Eisenberg D. (1999) A census of protein repeats. Mol Biol,293:151-160.
Mengiste T, Chen X, Salmeron J, Dietrich R. (2003) The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis. Plant Cell,15:2551-2565.
Michaely P, Bennett V. (1992) The ANK repeat:A ubiquitous motif involved in macromolecular recognition. Trends Cell Biol,2:127-129.
Michaely P, Tomchick DR, Machius M, Anderson RG.(2002) Crystal structure of a 12 ANK repeat stack from human ankyrinR. EMBO,21:6387-6396.
Michel F, Soler Lopez M, Petosa C, Cramer P, Siebenlist U, Muller CW. (2001) Crystal structure of the ankyrin repeat domain of Bcl-3:A unique member of the IkB protein family. EMBO,20:6180-6190.
Mosavi LK, Cammett TJ, Desrosiers DC, Peng ZY. (2004) The ankyrin repeat as molecular architecture for protein recognition. Protein Sci,13:1435-1448.
Mosavi LK, Minor DL, Jr, Peng ZY. (2002a) Consensus-derived structural determinants of the ankyrin repeat motif. Proc Natl Acad Sci USA,99:16029-16034.
Mosavi LK, Williams S, Peng ZY. (2002b) Equilibrium folding and stability of myotrophin:A model ankyrin repeat protein. Mol Biol,320:165-170.
Mur LAJ, Kenton P, Atzorn R, Miersch O, Wasternack C. (2006) The outcomes of concentration-specific interactions between salicylate and jasmonate signaling include synergy, antagonism, and oxidative stress leading to cell death. Plant Physiol,140:249-262.
Nodzon LA, Xu WH, Wang Y, Pi LY, Chakrabarty PK, Song WY. (2004) The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis. Plant J,40:996-1006.
Norberg M, Holmlund M, Nilsson O. (2005) The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs. Development,132:2203-2213.
Nowak K, Luniak N, Witt C, Wustefeld Y, Wachter A. (2004) Peroxisomal localization of sulfite oxidase separates it from chloroplast-based sulfur assimilation. Plant Cell Physiol, 45:1889-1894.
Ortega S, Malumbres M, Barbacid M. (2002) Cyclin D-dependent kinases, INK4 inhibitors and cancer. Biochim BiophysActa,1602:73-87.
Pastori GM, Rio LA. (1997) Natural senescence of pea leaves:an activated oxygen-mediated function for peroxisomes. Plant Physiol,113:411-418.
Pratelli R, Lacombe B, Torregrosa L, Gaymard F, Romieu C, Thibaud JB, Sentenac H. (2002) A grapevine gene encoding a guard cell K(+) channel displays developmental regulation in the grapevine berry. Plant Physiol,128:564-577.
Pieterse CMJ, Van Wees SCM, Van Pelt JA, Knoester M, Laan R, Gerrits H,Weisbeek PJ, Van Loon LC. (1998) A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell,10:1571-1580.
Pozo MJ, Vander Ent S, Van Loon LC, Pieterse CMJ. (2008) Transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis thaliana. New Phytol,180:511-523.
Ralf R, Mendel. (2007) Biology of the molybdenum cofactor. Journal of Experimental Botany, 58:2289-2296.
Robert Hansch, Ralf R, Mendel. (2005) Sulfite oxidation in plant peroxisomes. Photosynthesis Research,86:337-343.
Ross AF. (1961) Systemic acquired resistance induced by localized virus infectionsin plants. Virology,14:340-358.
Sakamoto H, Matsuda O, Iba K. (2008) ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana. Plant J,56:411-422.
Sandhu D, Tasma IM, Frasch R, Bhattacharyya MK. (2009) Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1. BMC Plant Biol, 9:105.
Schrader N, Fischer K, Theis K, Mendel RR, Schwarz G, Kisker C. (2003) The crystal structure of plant sulfite oxidase provides insights into sulfite oxidation in plants and animals. Structure, 11:1251-1263.
Scheerer U, Haensch R, Mendel RR, Kopriva S, Rennenberg H, Herschbach C. (2010) Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5'-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing gamma-ECS, SO, or APR. JExp Bot,61:609-622.
Schwarz G, Mendel RR. (2006) Molybdenum cofactor biosynthesis and molybdenum enzymes. Annu Rev Plant Biol,57:623-647.
Seay M, Hayward AP, Tsao J, Dinesh Kumar SP. (2009) Something old, something new:plant innate immunity and autophagy. Curr Top Microbiol Immunol,335:287-306.
Sedgwick SG, Smerdon SJ. (1999) The ankyrin repeat:A diversity of interactions on a common structural framework. Trends Biochem Sci,24:311-316.
Segarra G, Vander Ent S, Trillas I, Pieterse CMJ. (2009) MYB72, a node of convergence in induced systemic resistance triggered by a fungal and a bacterial beneficial microbe. Plant Biol, 11:90-96.
Seong ES, Choi D, Cho HS, Lim CK, Cho HJ, Wang MH. (2007) Characterization of a stress-responsive ankyrin repeat-containing zinc finger protein of Capsicum annuum (CaKR1). J Biochem Mol Biol,40:952-958.
Shen G, Kuppu S, Venkataramani S, Wang J, Yan J, Qiu X, Zhang H. (2010) ANKYRIN REPEAT-CONTAINING PROTEIN 2A is an essential molecular chaperone for peroxisomal membrane-bound ASCORBATE PEROXIDASE3 in Arabidopsis. Plant Cell,22:811-831.
Sherr CJ, Roberts JM. (2004) Living with or without cyclins and cyclin-dependent kinases. Genes De,18:2699-2711.
Song F, Goodman RM. (2002a) OsBIMK1, a rice MAP kinase gene involved in disease resistance responses. Planta,215:997-1005.
Song F, Goodman RM. (2002b) Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1 that is activated in systemic acquired resistance. Physiol Mol Plant Pathol,61:31-40.
Stolz JF, Basu P. (2002) Evolution of nitrate reductase:molecular and structural variations on a common function. Chem Biochem,3:198-206.
Ton J, De Vos M, Robben C, Buchala A, Metraux JP, Van Loon LC, Pieterse CMJ. (2002) Characterization of Arabidopsis enhanced disease susceptibility mutants that are affected in systemically induced resistance. Plant J,29:11-21.
Tang KS, Guralnick BJ, Wang WK, Fersht AR, Itzhaki LS. (1999) Stability and folding of the tumour suppressor protein p16. Mol Biol,285:1869-1886.
Tevelev A, Byeon IJ, Selby T, Ericson K, Kim HJ, Kraynov V, Tsai MD. (1996) Tumor suppressor p16INK4A:Structural characterization of wild-type and mutant proteins by NMR and circular dichroism. Biochemistry,35:9475-9487.
Thompson JD, Higgins DG, Gibson TJ. (1994) CLUSTALW:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Res,22:4673-4680.
Tian Wu, Zhendong Tian, Jun Liu, Chunguang Yao, Conghua Xie. (2009) A Novel Ankyrin Repeat-rich Gene in Potato, Star, Involved in Response to Late Blight. Biochem Genet, 47:439-450.
Truman W, Bennett MH, Kubigsteltig I, Turnbull C, Grant M. (2007) Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates. Proc Natl Acad Sci USA 104:1075-1080.
Van Loon LC, Bakker PAHM. (2006) Root-associated bacteria inducing systemic resistance. Plant-Associated Bacteria,269-316.
Van Wees SCM, Vander Ent S, Pieterse CMJ. (2008) Plant immune responses triggered by beneficial microbes. Curr Opin Plant Biol,11:443-448.
Vander Ent S, Van Wees SC, Pieterse CM. (2009) Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes. Phytochemistry,70:1581-1588.
Veronese P, Nakagami H, Bluhm B, Abuqamar S, Chen X, Salmeron J, Dietrich RA, Hirt H, Mengiste T, Paola V, Hirofumi N, Burton B, Synan AQ, Xi C, John S,Robert AD, Heribert H, Tesfaye M. (2006) The Membrane-Anchored BOTRYTIS-INDUCED KINASE1 Plays Distinct Roles in Arabidopsis Resistance to Necrotrophic and Biotrophic Pathogens. Plant Cell,18:257-273.
Walke RG, Willingham AT, Zuker CS. (2000) A Drosophila mechanosensory transduction channel. Science,287:2229-2234.
Wang YS, Pi LY, Chen X, Chakrabarty PK, Jiang J, De Leon AL, Liu GZ, Li L, Benny U, Oard J, Ronald PC, Song WY. (2006) Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance. Plant Cell,18:3635-3646.
Wolff B, Naumann M. (1999) INK4 cell cycle inhibitors direct transcriptional inactivation of NF-kB. Oncogene,18:2663-2666.
Yan J, Wang J, Zhang H. (2002) An ankyrin repeat-containing protein plays a role in both disease resistance and antioxidation metabolism. Plant J,29:193-202.
Yang Y, Nanduri S, Sen S, Qin J. (1998) The structural basis of ankyrin-like repeat function as revealed by the solution structure of myotrophin. Structure,15:619-626.
Yu B, Xu C, Benning C. (2002) Arabidopsis disrupted in SQD2 encoding sulfolipid synthase is impaired in phosphate-limited growth. Proc Natl Acad Sci USA,99:5732-5737.
Yuan C, Li J, Mahajan A, Poi MJ, Byeon IJ, Tsai MD. (2004) Solution structure of the human oncogenic protein gankyrin containing seven ankyrin repeats and analysis of its structure function relationship. Biochemistry,43:12152-12161.
Zhang B, Peng ZY. (2000) A minimum folding unit in the ankyrin repeat protein p16(INK4). Mol Biol,299:1121-1132.
Zhang H, Scheirer DC, Fowle WH, Goodman HM. (1992) Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in arabidopsis. Plant Cell,4:1575-1588.
Zhang X, Li D, Zhang H, Wang X, Zheng Z, Song F. (2010) Molecular characterization of rice OsBIANK1, encoding a plasma membrane-anchored ankyrin repeat protein, and its inducible expression in defense responses. Mol Biol Rep,37:653-660.
张治礼.2008植物亚硫酸氧化酶及其基因研究进展.安徽农学通报.,14(11).
吴利民,李东屏.2005植物锚蛋白研究进展.生物技术通报.,6.
Ahmad A, Sarfraz A. (2010) Screening and partial immunochemical characterization of sulfite oxidase from plant source. Indian JExp Biol,48:83-86.
Albert S, Despres B, Guilleminot J, Bechtold N, Pelletier G, Delseny M, Devic M. (1999) The EMB 506 gene encodes a novel ankyrin repeat containing protein that is essential for the normal development of Arabidopsis embryos. Plant J,17:169-179.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. (1997) Gapped BLAST and PSI-BLAST:a new generation of protein database search programs. Nucleic Acids Res,25:3389-3402.
Andrade MA, Perez Iratxeta C, Ponting CP. (2001) Protein repeats:Structures, functions, and evolution. Struct Biol,134:117-131.
Astashkin AV, Johnson Winters K, Klein EL, Byrne RS, Hille R, Raitsimring AM, Enemark JH. (2007) Direct demonstration of the presence of coordinated sulfate in the reaction pathway of Arabidopsis thaliana sulfite oxidase using 33S labeling and ESEEM spectroscopy. J Am Chem Soc,129:14800-14810.
Attaran E, Zeier TE, Griebel T, Zeier J. (2009) Systemic acquired resistance in Arabidopsis is independent of methyl salicylate production and jasmonate signaling. Plant Cell,21:954-971.
Bae W, Lee YJ, Kim DH, Lee J, Kim S, Sohn EJ, Hwang I. (2008) AKR2A-mediated import of chloroplast outer membrane proteinsis essential for chloroplast biogenesis. Nat Cell Biol, 10:220-227.
Batchelor AH, Piper DE, dela Brousse FC, McKnight SL, Wolberger C. (1998) The structure of GABPalpha/beta:an ETS domain- ankyrin repeat heterodimer bound to DNA. Science, 13:1037-1041.
Breeden L, Nasmyth K. (1987) Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila. Nature,329:651-654.
Brychkova G, Xia Z, Yang G, Yesbergenoval Z, Zhang Z, Davydov O, Fluhr R, Sagi M1. (2007) Sulfite Oxidase protects plants against sulfur dioxide t oxicity. Plant J,50:696-709.
Byeon IJ, Li J, Ericson K, Selby TL, Tevelev A, Kim HJ, O'Maille P, Tsai MD. (1998)Tumor suppressor p16INK4A:Determination of solution structure and analyses of its interaction with cyclin-dependent kinase 4. Mol Cell,1:421-431.
Cao H, Glazebrook J, Clarke JD, Volko S, Dong X. (1997) The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell, 88:57-63.
Chern M, Fitzgerald HA, Canlas PE, Navarre DA, Ronald PC. (2005) Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact,18:511-520.
Choi BY, Choi HS, Ko K, Cho YY, Zhu F, Kang BS, Ermakova SP, Ma WY, Bode AM, Dong Z. (2005) The tumor suppressor p16(INK4a) prevents cell transformation through inhibition of c-Jun phosphorylation and AP-1 activity. Nat Struct Mol Biol,12:699-707.
Choi MS, Kim MC, Yoo JH, Moon BC, Koo SC, Park BO, Lee JH, Koo YD, Han HJ, Lee SY, Chung WS, Lim CO, Cho MJ. (2005) Isolation of a calmodulin-binding transcription factor from rice(Oryza sativa L. J Biol Chem,280:40820-40831.
Clough SJ, Bent AF. (1998) Floral dip:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J,16:735-743.
Cohen HJ, Betcher Lange S, Kessler DL, Rajagopalan KV. (1972) Hepatic sulfite oxidase. Congruency in mitochondria of prosthetic groups and activity. J Biol Chem,247:7759-7766.
Coleman KG, Wautlet BS, Morrissey D, Mulheron J, Sedman SA, Brinkley P, Price S, Webster KR. (1997) Identification of CDK4 sequences involved in cyclin D1 and p16 binding. Biol Chem,272:18869-18874.
Cui J, Bahrami AK, Pringle EG, Hernandez Guzman G, Bender CL, Pierce NE, Ausubel FM. (2005) Pseudomonas syringae manipulates systemic plant defenses against pathogens and herbivores. Proc Nat Acad Sci USA,102:1791-1796.
Dai X, Hayashi K, NozakiH, Cheng Y, Zhao Y. (2005) Genetic and chemical analyses of the actionmechanisms of sirtinol in Arabidopsis. Proc Natl Acad Sci USA,102:3129-3134.
Dawson S, Apcher S, Mee M, Higashitsuji H, Baker R, Uhle S, Dubiel W, Fujita J, Mayer RJ. (2002) Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome. Biol Chem,277:10893-10902.
De Vos M, Van Oosten VR, Van Poecke RMP, Van Pelt JA, Pozo MJ, Mueller MJ, Buchala AJ, Metraux JP, Van Loon LC, Dicke M. (2005) Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol Plant Microbe Interact,18:923-937.
Dong X. (2004) NPR1, all things considered. Curr Opin Plant Biol,7:547-552.
Durrant WE, Dong X. (2004) Systemic acquired resistance; Annu Rev Phytopathol,42:185-209.
Eilers T, Schwarz G, Brinkmann H, Witt C, Richter T, Nieder J, Koch B, Hille R, Hansch R, Mendel RR. (2001) Identification and biochemical characterization of Arabidopsis thaliana sulfite bxidase. A new player in plant sulfur metabolism. J Biol Chem,276:46989-46994.
Fitzgerald HA, Chern MS, Navarre R, Ronald PC. (2004) Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype. Mol Plant Microbe Interact,17:140-151.
Foord R, Taylor IA, Sedgwick SG, Smerdon SJ. (1999) X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function. Nat Struct Biol,6:157-165.
Forrer P, Binz HK, Stumpp MT, Pluckthun A. (2004) Consensus design of repeat proteins. Chem BioChem,5:183-189.
Forrer P, Stumpp MT, Binz HK, Pluckthun A. (2003) A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett,539:2-6.
Garcion C, Guilleminot J, Kroj T, Parcy F, Giraudat J, Devic M. (2006) AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis. Plant J,48:895-906.
Gohre V, Robatzek S. (2008) Breaking the barriers:microbial effector molecules subvert plant immunity. Annu Rev Phytopathol,46:189-215.
Gunter Schwarz, Ralf R, Mendel. (2006) Molybdenum Cofactor Biosynthesis and Molybdenum Enzymes. Annu Rev Plant Biol,57:623-647.
Ha CM, Jun JH, Nam HG, Fletcher JC. (2004) BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana. Plant Cell Physiol, 45:1361-1370.
Haenzelmann P, Hernandez HL, Menzel C, Garcia-Serres R, Huynh BH. (2004) Characterization of MOCS1A, an oxygen-sensitive iron-sulfur protein involved in human molybdenum cofactor biosynthesis. J Biol Chem,279:34721-34732.
Hansch R, Lang C, Rennenberg H, Mendel RR. (2007) Significance of plant sulfite oxidase. Plant Biol,9:589-595.
Heber U, H"uve K. (1998) Action of SO2 on plants and metabolic detoxification of SO2. Intl Rev Cytol,177:255-286.
Hemsley PA, Kemp AC, Grierson CS. (2005) The TIP GROWTH DEFECTIVE1 S-acyl transferase regulates plant cell growth in Arabidopsis. Plant Cell,17:2554-2563.
Huang J, Chen F, Del Casino C, Autino A, Shen M, Yuan S, Peng J, Shi H, Wang C, Cresti M, Li Y. (2006) An ankyrin repeat-containing protein, characterized as a ubiquitin ligase, is closely associated with membrane-enclosed organelles and required for pollen germination and pollen tube growth in lily. Plant Physiol,140:1374-1383.
Huxford T, Huang DB, Malek S, Ghosh G.(1998) The crystal structure of the IkBa/NF-kB complex reveals mechanisms of NF-kB inactivation. Cell,95:759-770.
Jianyan Huang, Xiaobo Zhao, Huihui Yu, Yidan Ouyang, Lei Wang, Qifa Zhang. (2009) The ankyrin repeat gene family in rice:genome-wide identification, classification and expression profiling. Plant Mol Biol,71:207-226.
Johnson JL, Rajagopalan KV. (1979) The oxidation of sulphite in animals systems. Ciba Found Symp,72:119-133.
Junan Li, Anjali Mahajan, Ming Daw Tsai. (2006) Ankyrin Repeat:A Unique Motif Mediating Protein-Protein Interactions. Biochemistry,45:15168-15178.
Katagirl F, Thilmony, He SY. (2002) The Arabidopsis Thaliana-Pseudomonas Syringae Interaction. The Arabidopsis Book, doi:10.1199/tab.0039.
Kim MG, Kim SY, Kim WY, Mackey D, Lee SY. (2008) Responses of Arabidopsis thaliana to challenge by Pseudomonas syringae. Mol Cells,25:323-331.
Klimyuk VI, Persello Cartieaux F, Havaux M, Contard David P, Schuenemann D, Meiherhoff K, Gouet P, Jones JD, Hoffman NE, Nussaume L. (1999) A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell,11:87-99.
Kohl A, Binz HK, Forrer P, Stumpp MT, Pluckthun A, Grutter MG (2003) Designed to be stable:Crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci USA,100; 1700-1705.
Koizumi K, Naramoto S, Sawa S, Yahara N, Ueda T, Nakano A, Sugiyama M, Fukuda H. (2005) VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation. Development,132:1699-1711.
Lapointe J, Lachance Y, Labrie Y, Labrie C. (1996) A p18 mutant defective in CDK6 binding in human breast cancer cells. Cancer Res,56:4586-4589.
Leon Reyes A, Spoel SH, De Lange ES, Abe H, Kobayashi M, Tsuda S, Millenaar FF, Welschen RA, Ritsema T, Pieterse CM. (2009) Ethylene modulates the role of NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 in cross talk between salicylate and jasmonate signaling. Plant Physiol,149:1797-1809.
Leustek T, Saito K. (1999) Sulfate transport and assimilation in plants. Plant Physiol, 120:637-644.
Li HY, Chye ML. (2004) Arabidopsis Acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats. Plant Mol Biol, 54:233-243.
Li J, Joo SH, Tsai MD. (2003) An NF-kB-specific inhibitor, IkBa, binds to and inhibits cyclin-dependent kinase 4. Biochemistry,42:13476-13483.
Li J, Poi MJ, Qin D, Selby TL, Byeon IJ, Tsai MD. (2000) Tumor suppressor INK4:Quantitative structure-function analyses of p18INK4C as an inhibitor of cyclin-dependent kinase 4. Biochemistry,39:649-657.
Li J, Tsai MD. (2002) Novel insights into the INK4-CDK4/6-Rb pathway:Counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb. Biochemistry,41:3977-3983.
Li X, Zhang Y, Clarke DJ, Li Y, Dong X. (1999) Identification and cloning of a negative regulator of systemic acquired resistance, SNI1, through a screen for suppressors of nprl-1. Cell,98: 329-339.
Loar JW, Seiser RM, Sundberg AE, Sagerson HJ, Ilias N, Zobel-Thropp P, Craig EA, Lycan DE. (2004) Genetic and biochemical interactions among Yarl, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cere isiae. Genetics,168:1877-1889.
Lux SE, John KM, Bennett V. (1990) Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins. Nature,344:36-42.
Lycan DE, Stafford KA, Bollinger W, Breeden LL. (1996) A new Saccharomyces cere isiae ankyrin repeat-encoding gene required for a normal rate of cell proliferation. Gene,171:33-40.
MacKintosh C, Meek SE. (2001) Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis. Cell Mol Life Sci,58:205-214.
Main ER, Jackson SE, Regan L. (2003) The folding and design of repeat proteins:Reaching a consensus. Curr Opin Struct Biol,13:482-489.
Marcotte EM, Pellegrini M, Yeates TO, Eisenberg D. (1999) A census of protein repeats. Mol Biol,293:151-160.
Mengiste T, Chen X, Salmeron J, Dietrich R. (2003) The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis. Plant Cell,15:2551-2565.
Michaely P, Bennett V. (1992) The ANK repeat:A ubiquitous motif involved in macromolecular recognition. Trends Cell Biol,2:127-129.
Michaely P, Tomchick DR, Machius M, Anderson RG.(2002) Crystal structure of a 12 ANK repeat stack from human ankyrinR. EMBO,21:6387-6396.
Michel F, Soler Lopez M, Petosa C, Cramer P, Siebenlist U, Muller CW. (2001) Crystal structure of the ankyrin repeat domain of Bcl-3:A unique member of the IkB protein family. EMBO,20:6180-6190.
Mosavi LK, Cammett TJ, Desrosiers DC, Peng ZY. (2004) The ankyrin repeat as molecular architecture for protein recognition. Protein Sci,13:1435-1448.
Mosavi LK, Minor DL, Jr, Peng ZY. (2002a) Consensus-derived structural determinants of the ankyrin repeat motif. Proc Natl Acad Sci USA,99:16029-16034.
Mosavi LK, Williams S, Peng ZY. (2002b) Equilibrium folding and stability of myotrophin:A model ankyrin repeat protein. Mol Biol,320:165-170.
Mur LAJ, Kenton P, Atzorn R, Miersch O, Wasternack C. (2006) The outcomes of concentration-specific interactions between salicylate and jasmonate signaling include synergy, antagonism, and oxidative stress leading to cell death. Plant Physiol,140:249-262.
Nodzon LA, Xu WH, Wang Y, Pi LY, Chakrabarty PK, Song WY. (2004) The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis. Plant J,40:996-1006.
Norberg M, Holmlund M, Nilsson O. (2005) The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs. Development,132:2203-2213.
Nowak K, Luniak N, Witt C, Wustefeld Y, Wachter A. (2004) Peroxisomal localization of sulfite oxidase separates it from chloroplast-based sulfur assimilation. Plant Cell Physiol, 45:1889-1894.
Ortega S, Malumbres M, Barbacid M. (2002) Cyclin D-dependent kinases, INK4 inhibitors and cancer. Biochim BiophysActa,1602:73-87.
Pastori GM, Rio LA. (1997) Natural senescence of pea leaves:an activated oxygen-mediated function for peroxisomes. Plant Physiol,113:411-418.
Pratelli R, Lacombe B, Torregrosa L, Gaymard F, Romieu C, Thibaud JB, Sentenac H. (2002) A grapevine gene encoding a guard cell K(+) channel displays developmental regulation in the grapevine berry. Plant Physiol,128:564-577.
Pieterse CMJ, Van Wees SCM, Van Pelt JA, Knoester M, Laan R, Gerrits H,Weisbeek PJ, Van Loon LC. (1998) A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell,10:1571-1580.
Pozo MJ, Vander Ent S, Van Loon LC, Pieterse CMJ. (2008) Transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis thaliana. New Phytol,180:511-523.
Ralf R, Mendel. (2007) Biology of the molybdenum cofactor. Journal of Experimental Botany, 58:2289-2296.
Robert Hansch, Ralf R, Mendel. (2005) Sulfite oxidation in plant peroxisomes. Photosynthesis Research,86:337-343.
Ross AF. (1961) Systemic acquired resistance induced by localized virus infectionsin plants. Virology,14:340-358.
Sakamoto H, Matsuda O, Iba K. (2008) ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana. Plant J,56:411-422.
Sandhu D, Tasma IM, Frasch R, Bhattacharyya MK. (2009) Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1. BMC Plant Biol, 9:105.
Schrader N, Fischer K, Theis K, Mendel RR, Schwarz G, Kisker C. (2003) The crystal structure of plant sulfite oxidase provides insights into sulfite oxidation in plants and animals. Structure, 11:1251-1263.
Scheerer U, Haensch R, Mendel RR, Kopriva S, Rennenberg H, Herschbach C. (2010) Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5'-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing gamma-ECS, SO, or APR. JExp Bot,61:609-622.
Schwarz G, Mendel RR. (2006) Molybdenum cofactor biosynthesis and molybdenum enzymes. Annu Rev Plant Biol,57:623-647.
Seay M, Hayward AP, Tsao J, Dinesh Kumar SP. (2009) Something old, something new:plant innate immunity and autophagy. Curr Top Microbiol Immunol,335:287-306.
Sedgwick SG, Smerdon SJ. (1999) The ankyrin repeat:A diversity of interactions on a common structural framework. Trends Biochem Sci,24:311-316.
Segarra G, Vander Ent S, Trillas I, Pieterse CMJ. (2009) MYB72, a node of convergence in induced systemic resistance triggered by a fungal and a bacterial beneficial microbe. Plant Biol, 11:90-96.
Seong ES, Choi D, Cho HS, Lim CK, Cho HJ, Wang MH. (2007) Characterization of a stress-responsive ankyrin repeat-containing zinc finger protein of Capsicum annuum (CaKR1). J Biochem Mol Biol,40:952-958.
Shen G, Kuppu S, Venkataramani S, Wang J, Yan J, Qiu X, Zhang H. (2010) ANKYRIN REPEAT-CONTAINING PROTEIN 2A is an essential molecular chaperone for peroxisomal membrane-bound ASCORBATE PEROXIDASE3 in Arabidopsis. Plant Cell,22:811-831.
Sherr CJ, Roberts JM. (2004) Living with or without cyclins and cyclin-dependent kinases. Genes De,18:2699-2711.
Song F, Goodman RM. (2002a) OsBIMK1, a rice MAP kinase gene involved in disease resistance responses. Planta,215:997-1005.
Song F, Goodman RM. (2002b) Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1 that is activated in systemic acquired resistance. Physiol Mol Plant Pathol,61:31-40.
Stolz JF, Basu P. (2002) Evolution of nitrate reductase:molecular and structural variations on a common function. Chem Biochem,3:198-206.
Ton J, De Vos M, Robben C, Buchala A, Metraux JP, Van Loon LC, Pieterse CMJ. (2002) Characterization of Arabidopsis enhanced disease susceptibility mutants that are affected in systemically induced resistance. Plant J,29:11-21.
Tang KS, Guralnick BJ, Wang WK, Fersht AR, Itzhaki LS. (1999) Stability and folding of the tumour suppressor protein p16. Mol Biol,285:1869-1886.
Tevelev A, Byeon IJ, Selby T, Ericson K, Kim HJ, Kraynov V, Tsai MD. (1996) Tumor suppressor p16INK4A:Structural characterization of wild-type and mutant proteins by NMR and circular dichroism. Biochemistry,35:9475-9487.
Thompson JD, Higgins DG, Gibson TJ. (1994) CLUSTALW:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Res,22:4673-4680.
Tian Wu, Zhendong Tian, Jun Liu, Chunguang Yao, Conghua Xie. (2009) A Novel Ankyrin Repeat-rich Gene in Potato, Star, Involved in Response to Late Blight. Biochem Genet, 47:439-450.
Truman W, Bennett MH, Kubigsteltig I, Turnbull C, Grant M. (2007) Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates. Proc Natl Acad Sci USA 104:1075-1080.
Van Loon LC, Bakker PAHM. (2006) Root-associated bacteria inducing systemic resistance. Plant-Associated Bacteria,269-316.
Van Wees SCM, Vander Ent S, Pieterse CMJ. (2008) Plant immune responses triggered by beneficial microbes. Curr Opin Plant Biol,11:443-448.
Vander Ent S, Van Wees SC, Pieterse CM. (2009) Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes. Phytochemistry,70:1581-1588.
Veronese P, Nakagami H, Bluhm B, Abuqamar S, Chen X, Salmeron J, Dietrich RA, Hirt H, Mengiste T, Paola V, Hirofumi N, Burton B, Synan AQ, Xi C, John S,Robert AD, Heribert H, Tesfaye M. (2006) The Membrane-Anchored BOTRYTIS-INDUCED KINASE1 Plays Distinct Roles in Arabidopsis Resistance to Necrotrophic and Biotrophic Pathogens. Plant Cell,18:257-273.
Walke RG, Willingham AT, Zuker CS. (2000) A Drosophila mechanosensory transduction channel. Science,287:2229-2234.
Wang YS, Pi LY, Chen X, Chakrabarty PK, Jiang J, De Leon AL, Liu GZ, Li L, Benny U, Oard J, Ronald PC, Song WY. (2006) Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance. Plant Cell,18:3635-3646.
Wolff B, Naumann M. (1999) INK4 cell cycle inhibitors direct transcriptional inactivation of NF-kB. Oncogene,18:2663-2666.
Yan J, Wang J, Zhang H. (2002) An ankyrin repeat-containing protein plays a role in both disease resistance and antioxidation metabolism. Plant J,29:193-202.
Yang Y, Nanduri S, Sen S, Qin J. (1998) The structural basis of ankyrin-like repeat function as revealed by the solution structure of myotrophin. Structure,15:619-626.
Yu B, Xu C, Benning C. (2002) Arabidopsis disrupted in SQD2 encoding sulfolipid synthase is impaired in phosphate-limited growth. Proc Natl Acad Sci USA,99:5732-5737.
Yuan C, Li J, Mahajan A, Poi MJ, Byeon IJ, Tsai MD. (2004) Solution structure of the human oncogenic protein gankyrin containing seven ankyrin repeats and analysis of its structure function relationship. Biochemistry,43:12152-12161.
Zhang B, Peng ZY. (2000) A minimum folding unit in the ankyrin repeat protein p16(INK4). Mol Biol,299:1121-1132.
Zhang H, Scheirer DC, Fowle WH, Goodman HM. (1992) Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in arabidopsis. Plant Cell,4:1575-1588.
Zhang X, Li D, Zhang H, Wang X, Zheng Z, Song F. (2010) Molecular characterization of rice OsBIANK1, encoding a plasma membrane-anchored ankyrin repeat protein, and its inducible expression in defense responses. Mol Biol Rep,37:653-660.
张治礼.2008植物亚硫酸氧化酶及其基因研究进展.安徽农学通报.,14(11).
吴利民,李东屏.2005植物锚蛋白研究进展.生物技术通报.,6.