摘要
目的:通过观察孕酮(progesterone P)对离体家
兔窦房结细胞动作电位的影响,来探讨孕酮对窦房结细胞电活动
的影响及其作用机制。方法:采用微电极技术,引导窦房结细胞
动作电位,观察不同浓度孕酮对窦房结细胞动作电位各参数的影
响。结果:(1)孕酮使兔窦房结细胞动作电位时程(APD_(20)、APD_(50)、
APD_(90)、APD)、总复极时间(RPT)显著延长。孕酮对窦房结细胞
动作电位各参数的影响呈时间、浓度依赖性。此结果与我室以前
相关课题观察到的P(3×10~(-4)M)可显著延长豚鼠心室乳头肌细
胞动作电位APD_(90)和APD相吻合。(2)孕酮使兔窦房结细胞动作
电位幅度(APA)减小,零期最大除极速率(Vmax)减慢,使窦房
结细胞0期除极时间(DPT)延长。结论:结果显示,较高浓度
的孕酮对离体家兔窦房结细胞的K~+通道有一定程度的阻断作
用,对其Ca~(2+)通道可能也有阻断作用。
The effects of Progesterone(P) on isolated rabbit
sino atrial node pacemaker cells were studied with intracellular
microelectrode technique to investigate the changes of action
potential(AP) at iON 20N 40~. 60~ 80N 100 and 120 minutes after
administration with different doses of P. Results: P induced
prolongations of AP durations (APD20,APD50,APD90 and APD) and
the total repolarization time (phase 3 of AP) significantly in
concentration-dependent and time-dependent manners. P also
significantly decreased action potential amplitude(APA) and the
maximal velocity rate of depolarization of phase 0(Vmax) in
concentration and time dependent manners. All the results suggested
that P inhibits the K+ channels maybe also the Ca2+ channels of
rabbit sino atrial cells.
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