孕酮对兔窦房结细胞电生理特性的影响
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摘要
目的：通过观察孕酮（progesterone P）对离体家
兔窦房结细胞动作电位的影响，来探讨孕酮对窦房结细胞电活动
的影响及其作用机制。方法：采用微电极技术，引导窦房结细胞
动作电位，观察不同浓度孕酮对窦房结细胞动作电位各参数的影
响。结果：（1）孕酮使兔窦房结细胞动作电位时程（APD_(20)、APD_(50)、
APD_(90)、APD）、总复极时间（RPT）显著延长。孕酮对窦房结细胞
动作电位各参数的影响呈时间、浓度依赖性。此结果与我室以前
相关课题观察到的P（3×10~(-4)M）可显著延长豚鼠心室乳头肌细
胞动作电位APD_(90)和APD相吻合。（2）孕酮使兔窦房结细胞动作
电位幅度（APA）减小，零期最大除极速率（Vmax）减慢，使窦房
结细胞0期除极时间（DPT）延长。结论：结果显示，较高浓度
的孕酮对离体家兔窦房结细胞的K~+通道有一定程度的阻断作
用，对其Ca~(2+)通道可能也有阻断作用。
The effects of Progesterone(P) on isolated rabbit
sino atrial node pacemaker cells were studied with intracellular
microelectrode technique to investigate the changes of action
potential(AP) at iON 20N 40~. 60~ 80N 100 and 120 minutes after
administration with different doses of P. Results: P induced
prolongations of AP durations (APD20,APD50,APD90 and APD) and
the total repolarization time (phase 3 of AP) significantly in
concentration-dependent and time-dependent manners. P also
significantly decreased action potential amplitude(APA) and the
maximal velocity rate of depolarization of phase 0(Vmax) in
concentration and time dependent manners. All the results suggested
that P inhibits the K+ channels maybe also the Ca2+ channels of
rabbit sino atrial cells.
兔窦房结细胞动作电位的影响，来探讨孕酮对窦房结细胞电活动
的影响及其作用机制。方法：采用微电极技术，引导窦房结细胞
动作电位，观察不同浓度孕酮对窦房结细胞动作电位各参数的影
响。结果：（1）孕酮使兔窦房结细胞动作电位时程（APD_(20)、APD_(50)、
APD_(90)、APD）、总复极时间（RPT）显著延长。孕酮对窦房结细胞
动作电位各参数的影响呈时间、浓度依赖性。此结果与我室以前
相关课题观察到的P（3×10~(-4)M）可显著延长豚鼠心室乳头肌细
胞动作电位APD_(90)和APD相吻合。（2）孕酮使兔窦房结细胞动作
电位幅度（APA）减小，零期最大除极速率（Vmax）减慢，使窦房
结细胞0期除极时间（DPT）延长。结论：结果显示，较高浓度
的孕酮对离体家兔窦房结细胞的K~+通道有一定程度的阻断作
用，对其Ca~(2+)通道可能也有阻断作用。
The effects of Progesterone(P) on isolated rabbit
sino atrial node pacemaker cells were studied with intracellular
microelectrode technique to investigate the changes of action
potential(AP) at iON 20N 40~. 60~ 80N 100 and 120 minutes after
administration with different doses of P. Results: P induced
prolongations of AP durations (APD20,APD50,APD90 and APD) and
the total repolarization time (phase 3 of AP) significantly in
concentration-dependent and time-dependent manners. P also
significantly decreased action potential amplitude(APA) and the
maximal velocity rate of depolarization of phase 0(Vmax) in
concentration and time dependent manners. All the results suggested
that P inhibits the K+ channels maybe also the Ca2+ channels of
rabbit sino atrial cells.
引文
1． Stokesd J,et al.The relative importance of selected risk factors for various manifestations of cadiovascular disease among men and women from 35 to 64 years old: 30 years of follow-up in the Framingham Study .Circulation 1987 Jun;75(6Pt2) : V65-73
2． Folsom AR,et al.Hormonal replacement therapy and morbidity and mortality in a prospective study of postmeopausal wamen. AM T Public Health, 1995,85: 1128-1132133．
3． Larson JA,et al.Effects of gender on cardiac arrhythmias[see comments].J Cradiovasc Electrohpysiol,1998 Jun,9:6,655-64
4 . Stumf WE,et al.The heart:a target organ for estradiol. Science, 1977,196 :319-20
5. McGill HC Jr, et al. Nuclear uptake of sex steroids hormones in the cardiovascular system of the baboon. Circ Res 1981; 48:238-44
6. Sheridan PJ, et al. The nuclear uptake and retention of a synthetic progestin in the cardiovascular system of the baboon. Endocrinology, 1984 Jun, 114:6, 2015-9
7. Pasanen s, et al. Distribution of progesterone receptor in chicken: nove traget organs for progesterone and estrogen action. Mol Cell Endocrinol, 1997 Nov, 135:1, 79-91
8. Knauthe R, et al. Sexual dimorphism of steroid hormone receptor messenger ribonueleic acid expression and hormone regulation in rat vascular tissue Endocrinology, 1996 Aug: 137(8); 3220-7
9. Bush TL. Preserving cardiovascular benefits of hormone replacement therapy. J Reprod Med, 2000 03, 45:3 Suppl, 259-73
10. Moiler RA, et al. Effects of progesterone on the cardiac electrophysiology action of bupivacaine and lidoeaine. Anesthesiology, 1992 Apr, 76:4, 604-8
11．曹春梅等。孕酮对豚鼠心室乳头肌细胞电生理特性的影响。待发表
12. Irisawa H; Brown H.F. and Giles W; Cardiac Pacemaking in the sinoatrial node. Physiol. Rew 1993 73(1): 197-227
13．陈丽娜等。雌激素对兔窦房结细胞动作电位的影响。新疆医科大学学报 1999．22(3)
14. Ehring GR, et al. A nongenomic mechanism for progesterone-mediated immunusppression: inhibition of K~+ channel, Ca~(2+) signaling, and gene expresion in T lymphocyte. J Exp Med, 1998 Nov, 188:9, 1593-602
15. Ouadid Ahidouch H. Voltage-gated calcium channels in Pleurodeles oocytes: classification, modulation, and functional roles. Zygote, 1998 Feb, 6:1,85-95
16. Doerr, TR, Denger, and Trautwein W. Calcium currents in single SA nodal cells of the rabbit heart studied with action potential clamp. Pflueger Arch 1989. 413:599-603
17. Kreuzberg -U, et al. Single channel activity and expression of atrial L-type Ca~(2+) channels in patients with latent hyperthyroidism. Am J Physio Heart Circ Physiol, 2000 03, 278:3 H723-30
18. Raelclino-R, et al. Action of steroid sex hormones on beta-adrenergic and muscarinic receptors in rat heart. Life-Sci 1988: 42(23): 2307-14
2． Folsom AR,et al.Hormonal replacement therapy and morbidity and mortality in a prospective study of postmeopausal wamen. AM T Public Health, 1995,85: 1128-1132133．
3． Larson JA,et al.Effects of gender on cardiac arrhythmias[see comments].J Cradiovasc Electrohpysiol,1998 Jun,9:6,655-64
4 . Stumf WE,et al.The heart:a target organ for estradiol. Science, 1977,196 :319-20
5. McGill HC Jr, et al. Nuclear uptake of sex steroids hormones in the cardiovascular system of the baboon. Circ Res 1981; 48:238-44
6. Sheridan PJ, et al. The nuclear uptake and retention of a synthetic progestin in the cardiovascular system of the baboon. Endocrinology, 1984 Jun, 114:6, 2015-9
7. Pasanen s, et al. Distribution of progesterone receptor in chicken: nove traget organs for progesterone and estrogen action. Mol Cell Endocrinol, 1997 Nov, 135:1, 79-91
8. Knauthe R, et al. Sexual dimorphism of steroid hormone receptor messenger ribonueleic acid expression and hormone regulation in rat vascular tissue Endocrinology, 1996 Aug: 137(8); 3220-7
9. Bush TL. Preserving cardiovascular benefits of hormone replacement therapy. J Reprod Med, 2000 03, 45:3 Suppl, 259-73
10. Moiler RA, et al. Effects of progesterone on the cardiac electrophysiology action of bupivacaine and lidoeaine. Anesthesiology, 1992 Apr, 76:4, 604-8
11．曹春梅等。孕酮对豚鼠心室乳头肌细胞电生理特性的影响。待发表
12. Irisawa H; Brown H.F. and Giles W; Cardiac Pacemaking in the sinoatrial node. Physiol. Rew 1993 73(1): 197-227
13．陈丽娜等。雌激素对兔窦房结细胞动作电位的影响。新疆医科大学学报 1999．22(3)
14. Ehring GR, et al. A nongenomic mechanism for progesterone-mediated immunusppression: inhibition of K~+ channel, Ca~(2+) signaling, and gene expresion in T lymphocyte. J Exp Med, 1998 Nov, 188:9, 1593-602
15. Ouadid Ahidouch H. Voltage-gated calcium channels in Pleurodeles oocytes: classification, modulation, and functional roles. Zygote, 1998 Feb, 6:1,85-95
16. Doerr, TR, Denger, and Trautwein W. Calcium currents in single SA nodal cells of the rabbit heart studied with action potential clamp. Pflueger Arch 1989. 413:599-603
17. Kreuzberg -U, et al. Single channel activity and expression of atrial L-type Ca~(2+) channels in patients with latent hyperthyroidism. Am J Physio Heart Circ Physiol, 2000 03, 278:3 H723-30
18. Raelclino-R, et al. Action of steroid sex hormones on beta-adrenergic and muscarinic receptors in rat heart. Life-Sci 1988: 42(23): 2307-14