NF-κB对重症急性胰腺炎大鼠甲状旁腺激素受体表达和低钙血症的作用
|
摘要
目的探讨核转录因子(NF-κB)对重症急性胰腺炎(SAP)大鼠甲状旁腺激素受体(PTHR1)表达的作用和对低钙血症的影响。
方法将雄性SD大鼠72只随机分为3组,假手术组(SO)、SAP组、四氢化吡咯二硫代氨基甲酸脂(PDTC)预处理组(SAP+PDTC)各24只,以5%牛磺脱氧胆酸钠逆行胰胆管注射建立SAP模型,观察各组血清钙浓度以及胰腺病理改变,蛋白免疫印记技术(western blot)分析NF-κB表达量及甲状旁腺激素受体(PTHR1)表达量,逆转录-聚合酶链反应(RT-PCR)分析PTHR1 mRNA表达水平。
结果SAP组制模后NF-κB在早期迅速活化,于3h达到高峰;骨组织PTHR1的表达6h后出现显著的下调;血清钙浓度6h后显著降低(p<0.05)。与SAP组比较,PDTC预处理组血清钙水平明显升高,PTHR1的表达上调(P<0.01),NF-κB的峰值降低。
结论NF-κB可能通过对细胞因子的激活间接下调组织PTHR1的表达,致SAP血清钙显著下降。PDTC对改善SAP低钙血症有重要意义。
Objective To investigate the role of nuclear factor-kappa B(NF-κB) on the expression of parathyroid hormone receptor (PTHR1) and serum calcium level with severe acute pancreatitis (SAP) rats.
Methods 72 male Sprague-Dawley rats were randomly divided into sham operation group (SO), SAP group, pyrrolidine dithiocarbamat (PDTC) pretreated SAP group (SAP+PDTC), 24 rats each group. SAP model was developed by injecting 5% sodium taurodeoxycholate into biliary-pancreatic duct. Serum calcium, the expression of NF-κB and PTHR1 by western blot and the expression of PTHR1 by RT-PCR were determined at 0.5, 1, 3, 6 hour after operations.
Results NF-κB is activated soon after SAP and reaches the peak at 3 hour, then the expression of PTHR1 in bone and kidney down regulates. Serum levels of calcium is significantly reduced after 6 hour. As compared with SAP group, the level of NF-κB is decreased and the levels of PTHR1 and serum calcium are increased markedly in SAP+PDTC group.
Conclusion NF-κB may down regulate the expression of PTHR1 in bone and kidney through complicated interreactions among many kinds of cytokines, leading to the hypocalcaemia of SAP.
方法将雄性SD大鼠72只随机分为3组,假手术组(SO)、SAP组、四氢化吡咯二硫代氨基甲酸脂(PDTC)预处理组(SAP+PDTC)各24只,以5%牛磺脱氧胆酸钠逆行胰胆管注射建立SAP模型,观察各组血清钙浓度以及胰腺病理改变,蛋白免疫印记技术(western blot)分析NF-κB表达量及甲状旁腺激素受体(PTHR1)表达量,逆转录-聚合酶链反应(RT-PCR)分析PTHR1 mRNA表达水平。
结果SAP组制模后NF-κB在早期迅速活化,于3h达到高峰;骨组织PTHR1的表达6h后出现显著的下调;血清钙浓度6h后显著降低(p<0.05)。与SAP组比较,PDTC预处理组血清钙水平明显升高,PTHR1的表达上调(P<0.01),NF-κB的峰值降低。
结论NF-κB可能通过对细胞因子的激活间接下调组织PTHR1的表达,致SAP血清钙显著下降。PDTC对改善SAP低钙血症有重要意义。
Objective To investigate the role of nuclear factor-kappa B(NF-κB) on the expression of parathyroid hormone receptor (PTHR1) and serum calcium level with severe acute pancreatitis (SAP) rats.
Methods 72 male Sprague-Dawley rats were randomly divided into sham operation group (SO), SAP group, pyrrolidine dithiocarbamat (PDTC) pretreated SAP group (SAP+PDTC), 24 rats each group. SAP model was developed by injecting 5% sodium taurodeoxycholate into biliary-pancreatic duct. Serum calcium, the expression of NF-κB and PTHR1 by western blot and the expression of PTHR1 by RT-PCR were determined at 0.5, 1, 3, 6 hour after operations.
Results NF-κB is activated soon after SAP and reaches the peak at 3 hour, then the expression of PTHR1 in bone and kidney down regulates. Serum levels of calcium is significantly reduced after 6 hour. As compared with SAP group, the level of NF-κB is decreased and the levels of PTHR1 and serum calcium are increased markedly in SAP+PDTC group.
Conclusion NF-κB may down regulate the expression of PTHR1 in bone and kidney through complicated interreactions among many kinds of cytokines, leading to the hypocalcaemia of SAP.
引文
1. McMahon, M J. Woodhead, J S. Hayward, R.D., The nature of hypocalcaemia in acute pancreatitis. 1978. 65(3): p. 216-218.
2. 徐娴,孙士其,张振玉,樊克武, 重症急性胰腺炎核因子-κB及炎症介质与肺损伤的实验研究. 医学临床研究, 2003. 20(2): p. 84-87.
3. 王磊,于丽萍,窦科峰,王江, NF-κB在重症急性胰腺炎肝脏损伤中的作用. 中国现代医学杂志, 2004. 14(24): p. 51-53.
4. 王念林,陈垦,龙友明,兰雷,刘学进,王晖, 核因子-κB在大鼠急性坏死性胰腺炎中作用的实验研究. 胰腺病学, 2005. 5(2): p. 93-96.
5. 顾新华,李德春,谭纪伏,胡惠芳, 核因子-κB活化在重症急性胰腺炎肠道屏障损伤中的作用. 实用临床医学, 2006. 7(1): p. 4-8.
6. Tonn O , Kriegbaum S , Braitmaier A , et al. Expression-level dependent activation of recombinant human parathyroid hormone/ parathyroid hormone - related peptide receptor : effect of human parathyroid hormone ( 1-34) (1-31) and ( 28-48). Recept signal Transduct Res, 2000. 20: p. 133.
7. Fang Qian,Albert Leung, et al. Agonist-Dependent Phosphorylation of the Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor. Biochemistry, 1998. 37: p. 6240-6246.
8. Katz, M. S., Gutierrez, G. E., Mundy, G. R., Hymer, T. K., Caulfield, M. P.,and McKee, et al. Tumor necrosis factor and interleukin 1 inhibit parathyroid hormone-responsive adenylate cyclase in clonal osteoblast-like cells by downregulating parathyroid hormone receptors. Cell Physiol, 1992(153): p. 206 -213.
9. 丁卫,马硕,杜国光, 肿瘤坏死因子对大鼠成骨样细胞增殖与甲状旁腺素受体表达的影响. 生物化学杂志, 1997: p. 39-42.
10. E. TSUDA-FUTAMI, A. SHIOI, S. JONO, M. INABA, Y. NISHIZAWA, A.H.M., Lymphoblastoid Interferon-a Downregulates Parathyroid Hormone (PTH)/PTH-related Peptide (PTHrP) Receptor Expression in Human Osteoblastic Cells (Saos-2). Bone, 1998. 23: p. 205-211.
11. Tetsuya Kawane,Junsei Mimura,Yoshiaki Fujii-Kuriyama, et al. Identification of the promoter region of the parathyroid hormone receptor gene responsible for transcriptional suppression by insulin-like growth factor-I. Archives of Biochemistry and Biophysics, 2005. 439: p. 61-69.
12. Michael L.Picton,Philip R.Moore,E.Barbara Mawer,David Houghton,Anthony J.Freemont,Alastair J.Hutchison,Ram Gokal, et al. Down-regulation of human osteoblast PTH/PTHrP receptor mRNA in end-stage renal failure. Kidney International, 2000. 58: p. 1440-1449.
13. 黄鹤光,冷希圣, 重症急性胰腺炎大鼠骨组织甲状旁腺素受体mRNA的表达. 中华实验外科杂志, 2001(18): p. 303-304.
14. 陆逢春,黄鹤光, 重症急性胰腺炎肿瘤坏死因子-a与低钙血症的关系. 中华实验外科杂志, 2005(22): p. 397-398.
15. Chen Jing,Zhang Jian, et al. Down-regulation of PTH/PTHrP receptor in the kidney of patients with renal impairment. Chinese Medical Journal, 1998. 111: p. 24-27.
16. 雷若庆,韩天权,汤耀卿,袁祖荣,张圣道, TNFα在重症急性胰腺炎致病机制中的作用. 上海第二医科大学学报, 2000. 20(1): p. 1-4.
17. Weir GC. Lesser PB. Drop LJ. Fischer JE. Warshaw AL.The hypocalcemia of acute pancreatitis. Annals of Internal Medicine. 1975. 83(2): p. 185-189.
18. Imrie, C W. Beastall, G H.Allam, B F. O'Neil, J.Benjamin, I S. McKay, A.J., Parathyroid hormone and calcium homeostasis in acute pancreatitis. British Journal of Surgery, 1978. 65(10): p. 717-20.
19. Urena P,Mannstadt M,Hruby M,Ferreira A,Schmitt F,Silve C, et al. Parathyroidectomy does not prevent the renal PTH/PTHrP receptor downregulation in uraemic rats. Kidney Int, 1995. 47: p. 1797-1805.
20. Lin Y Xie,Albert Leung,Gino V Segre,Itsuo Yamamoto, et al. Down regulation of the PTH/PTHrP receptor by vitamin D3 in the osteoblast-like ROS17/2.8 cells. The American Physiological Society, 1996: p. 654-660.
21. McMahon, M J. Heyburn, P J. Playforth, M J.Woodhead, J.S., Parathyroid function during acute pancreatitis. British Journal of Surgery, 1982. 69(2): p. 95-98.
22. Seuwen K, et al. Heparin - insensitive calcium release from intracellular stores triggered by the recombinant human parathyroid hormone receptor. B r J Pharmacol, 1995. 114: p. 1613.
23. 周一农,黄鹤光, 甲强龙对重症急性胰腺炎大鼠低钙血症和甲状旁腺激素受体表达的影响. 中华实验外科杂志, 2006(23): p. 567-569.
24. Urena P,Lida-klein A, et al.Regulation of the PTH/PTHrP receptor mRNA by glucocorticoids and PTH in ROS17/2.8 and OK cells. Endocrinology, 1994: p. 135.
[1]刘新民主编。实用内分泌学第二版,人民军医出版社。1997:92-93。
[2]Hauser CJ. Kamrath RO. Sparks J. Shoemaker WC. Calcium homeostasis in patients with acute pancreatitis. Surgery. 1983 Nov. 94(5):830-835.
[3] Weir GC. Lesser PB. Drop LJ. Fischer JE. Warshaw AL. The hypocalcemia of acute pancreatitis. Annals of Internal Medicine. 1975 Aug, 83(2):185-189.
[4] McMahon, M J. Woodhead, J S. Hayward, R D. The nature of hypocalcaemia in acute pancreatitis.British Journal of Surgery. 1978 Mar, 65(3):216-218.
[5] Imrie, C W. Beastall, G H.Allam, B F. O'Neil, J. Benjamin, I S. McKay, A J. Parathyroid hormone and calcium homeostasis in acute pancreatitis. British Journal of Surgery. 1978 Oct, 65(10):717-20.
[6] McMahon, M J. Heyburn, P J. Playforth, M J.Woodhead, J S.Parathyroid function during acute pancreatitis. British Journal of Surgery. 1982 Feb, 69(2):95-98.
[7]Usdin TB, Gruber C, Bonner TI.Identification and functional expression of a receptor selectively recognizing parathyroid hormone,the PTH2 receptor. J Biol Chem ,1995,270:15455–15458.
[8] Miles RR , Sluka J P , Halladay DL , et al . Parathyroid hormone(hPTH 1～38) the expression of UBP41 , an ubiquitin-specific protease , in bone . Cell Biochem , 2002 , 85 : 229.
[9] Lee K C , Brown D , Urena P , et al. Localization of PTHrP receptor mRNA in kidney.Am J Physiol , 1996 , 270 : 186–191.
[10] Seuwen K, Boddeke HG..Heparin-insensitive calcium release from intracellular stores triggered by the recombinant human parathyroid hormone receptor . B r J Pharmacol , 1995 , 114 : 1613.
[11] Tonn O , Kriegbaum S , Braitmaier A , et al . Expression-leveldependent activation of recombinant human parathyroid hormone/ parathyroid hormone - related peptide receptor : effect of human parathyroid hormone ( 1-34) (1-31) and ( 28-48) .Recept signal Transduct Res , 2000 , 20: 133.
[12]Chen Jing,Zhang Jian,Lin Shanyan. Down-regulation of PTH/PTHrP receptor in the kidney of patients with renal impairment. Chinese Medical Journal,1998 ,111:24–27.
[13]陈靖,顾勇,张健,朱蔚钰,林善炎.硝苯地平和罗钙全对尿毒症患者甲状旁腺素受体基因表达的影响.中华肾脏病杂志,2000,16.
[14]Michael L.Picton,Philip R.Moore,E.Barbara Mawer,David Houghton,Anthony J.Freemont,Alastair J.Hutchison,Ram Gokal,Judith A.Hoyland. Down-regulation of human osteoblast PTH/PTHrP receptor mRNA in end-stage renal failure.Kidney International, 2000 , 58 :1440～1449.
[15]Fang Qian,Albert Leung,Abdul Abou-Samra.Agonist-Dependent Phosphorylation of the Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor. Biochemistry,1998 ,37:6240–6246.
[16]Urena P,Lida-klein A,Kong XF et al.Regulation of the PTH/PTHrP receptor mRNA by glucocorticoids and PTH in ROS17/2.8 and OK cells.Endocrinology.1994,135.
[17]Urena P,Mannstadt M,Hruby M,Ferreira A,Schmitt F,Silve C,Ardaillou R Lacour B Abou-samra A B segre GV.Drueke T.Parathyroidectomy does not prevent the renal PTH/PTHrP receptor downregulation in uraemic rats.Kidney Int 1995,47:1797-1805.
[18] Miroslaw Smogorzewski,Jin Tian,Shaul G Massry . Down-regulation of PTH/PTHrP receptor of heart in CRF:Role of [Ca2 +]i . Kidney International, 1995, 47 :1182～1186 .
[19] Miroslaw Smogorzewski,Wojciech Marcinkowski,Guoxiang Zhang,Shaul G..Massry. Parathyroidectomy does not affect mRNA of PTH/PTHrP receptor in kidney,liver and heart.Am J Nephrol1997,17:187-192.
[20]Lin Y Xie,Albert Leung,Gino V Segre,Itsuo Yamamoto,Abdul B Abou-samra . Down regulation of the PTH/PTHrP receptor by vitamin D3 in the osteoblast-like ROS17/2.8 cells. The American Physiological Society, 1996 : 654-660.
[21]Katz, M. S., Gutierrez, G. E., Mundy, G. R., Hymer, T. K., Caulfield, M. P.,and McKee, R. L. Tumor necrosis factor and interleukin 1 inhibit parathyroid hormone-responsive adenylate cyclase in clonal osteoblast-like cells by downregulating parathyroid hormone receptors. Cell Physiol ,1992,153:206 –213.
[22]丁卫,马硕,杜国光.肿瘤坏死因子对大鼠成骨样细胞增殖与甲状旁腺素受体表达的影响.生物化学杂志,1997,13:39-42.
[23]E. TSUDA-FUTAMI, A. SHIOI, S. JONO, M. INABA, Y. NISHIZAWA, and H. MORII. Lymphoblastoid Interferon-a Downregulates Parathyroid Hormone (PTH)/PTH-related Peptide (PTHrP) Receptor Expression in Human Osteoblastic Cells (Saos-2). Bone,1998, 23:205–211.
[24]Tetsuya Kawane,Junsei Mimura,Yoshiaki Fujii-Kuriyama,Noboru Horiuchi.Identification of the promoter region of the parathyroid hormone receptor gene responsible for transcriptional suppression by insulin-like growth factor-I. Archives of Biochemistry and Biophysics,2005,439:61-69.
[25]Jongen J,Willemstein Van Hove E et al.Down regulation of the receptor for parathyroid hormone(PTH)and PTH-related peptide by transforming growth factor β in primary fetal rat osteoblasts.Endocrinology.1995,136:3260-3266.
[26]杜国光,李平风,杨非易,顾文霞.甲状腺素及维甲酸对成骨细胞甲状旁腺素受体的调节作用.生物化学杂志,1997,13.
[27]Celic S,Chilco PJ,Zajac JD,Makin TJ,Findlay DM.A type I collagen substrate increases PTH/PTHrP receptor expression and suppresses PTHrPmRNA expression in UMR 106-06 osteoblast like cells. Endocrinology. 1996,150:299-308.
[28] 黄鹤光,冷希圣.重症急性胰腺炎大鼠骨组织甲状旁腺素受体 mRNA 的表达.中华实验外科杂志,2001,18:303-304.
[29] 陆逢春,黄鹤光.重症急性胰腺炎肿瘤坏死因子-a 与低钙血症的关系.中华实验外科杂志,2005,22:397-398.
[30]周一农,黄鹤光.甲强龙对重症急性胰腺炎大鼠低钙血症和甲状旁腺激素受体表达的影响.中华实验外科杂志,2006,23:567-569.
2. 徐娴,孙士其,张振玉,樊克武, 重症急性胰腺炎核因子-κB及炎症介质与肺损伤的实验研究. 医学临床研究, 2003. 20(2): p. 84-87.
3. 王磊,于丽萍,窦科峰,王江, NF-κB在重症急性胰腺炎肝脏损伤中的作用. 中国现代医学杂志, 2004. 14(24): p. 51-53.
4. 王念林,陈垦,龙友明,兰雷,刘学进,王晖, 核因子-κB在大鼠急性坏死性胰腺炎中作用的实验研究. 胰腺病学, 2005. 5(2): p. 93-96.
5. 顾新华,李德春,谭纪伏,胡惠芳, 核因子-κB活化在重症急性胰腺炎肠道屏障损伤中的作用. 实用临床医学, 2006. 7(1): p. 4-8.
6. Tonn O , Kriegbaum S , Braitmaier A , et al. Expression-level dependent activation of recombinant human parathyroid hormone/ parathyroid hormone - related peptide receptor : effect of human parathyroid hormone ( 1-34) (1-31) and ( 28-48). Recept signal Transduct Res, 2000. 20: p. 133.
7. Fang Qian,Albert Leung, et al. Agonist-Dependent Phosphorylation of the Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor. Biochemistry, 1998. 37: p. 6240-6246.
8. Katz, M. S., Gutierrez, G. E., Mundy, G. R., Hymer, T. K., Caulfield, M. P.,and McKee, et al. Tumor necrosis factor and interleukin 1 inhibit parathyroid hormone-responsive adenylate cyclase in clonal osteoblast-like cells by downregulating parathyroid hormone receptors. Cell Physiol, 1992(153): p. 206 -213.
9. 丁卫,马硕,杜国光, 肿瘤坏死因子对大鼠成骨样细胞增殖与甲状旁腺素受体表达的影响. 生物化学杂志, 1997: p. 39-42.
10. E. TSUDA-FUTAMI, A. SHIOI, S. JONO, M. INABA, Y. NISHIZAWA, A.H.M., Lymphoblastoid Interferon-a Downregulates Parathyroid Hormone (PTH)/PTH-related Peptide (PTHrP) Receptor Expression in Human Osteoblastic Cells (Saos-2). Bone, 1998. 23: p. 205-211.
11. Tetsuya Kawane,Junsei Mimura,Yoshiaki Fujii-Kuriyama, et al. Identification of the promoter region of the parathyroid hormone receptor gene responsible for transcriptional suppression by insulin-like growth factor-I. Archives of Biochemistry and Biophysics, 2005. 439: p. 61-69.
12. Michael L.Picton,Philip R.Moore,E.Barbara Mawer,David Houghton,Anthony J.Freemont,Alastair J.Hutchison,Ram Gokal, et al. Down-regulation of human osteoblast PTH/PTHrP receptor mRNA in end-stage renal failure. Kidney International, 2000. 58: p. 1440-1449.
13. 黄鹤光,冷希圣, 重症急性胰腺炎大鼠骨组织甲状旁腺素受体mRNA的表达. 中华实验外科杂志, 2001(18): p. 303-304.
14. 陆逢春,黄鹤光, 重症急性胰腺炎肿瘤坏死因子-a与低钙血症的关系. 中华实验外科杂志, 2005(22): p. 397-398.
15. Chen Jing,Zhang Jian, et al. Down-regulation of PTH/PTHrP receptor in the kidney of patients with renal impairment. Chinese Medical Journal, 1998. 111: p. 24-27.
16. 雷若庆,韩天权,汤耀卿,袁祖荣,张圣道, TNFα在重症急性胰腺炎致病机制中的作用. 上海第二医科大学学报, 2000. 20(1): p. 1-4.
17. Weir GC. Lesser PB. Drop LJ. Fischer JE. Warshaw AL.The hypocalcemia of acute pancreatitis. Annals of Internal Medicine. 1975. 83(2): p. 185-189.
18. Imrie, C W. Beastall, G H.Allam, B F. O'Neil, J.Benjamin, I S. McKay, A.J., Parathyroid hormone and calcium homeostasis in acute pancreatitis. British Journal of Surgery, 1978. 65(10): p. 717-20.
19. Urena P,Mannstadt M,Hruby M,Ferreira A,Schmitt F,Silve C, et al. Parathyroidectomy does not prevent the renal PTH/PTHrP receptor downregulation in uraemic rats. Kidney Int, 1995. 47: p. 1797-1805.
20. Lin Y Xie,Albert Leung,Gino V Segre,Itsuo Yamamoto, et al. Down regulation of the PTH/PTHrP receptor by vitamin D3 in the osteoblast-like ROS17/2.8 cells. The American Physiological Society, 1996: p. 654-660.
21. McMahon, M J. Heyburn, P J. Playforth, M J.Woodhead, J.S., Parathyroid function during acute pancreatitis. British Journal of Surgery, 1982. 69(2): p. 95-98.
22. Seuwen K, et al. Heparin - insensitive calcium release from intracellular stores triggered by the recombinant human parathyroid hormone receptor. B r J Pharmacol, 1995. 114: p. 1613.
23. 周一农,黄鹤光, 甲强龙对重症急性胰腺炎大鼠低钙血症和甲状旁腺激素受体表达的影响. 中华实验外科杂志, 2006(23): p. 567-569.
24. Urena P,Lida-klein A, et al.Regulation of the PTH/PTHrP receptor mRNA by glucocorticoids and PTH in ROS17/2.8 and OK cells. Endocrinology, 1994: p. 135.
[1]刘新民主编。实用内分泌学第二版,人民军医出版社。1997:92-93。
[2]Hauser CJ. Kamrath RO. Sparks J. Shoemaker WC. Calcium homeostasis in patients with acute pancreatitis. Surgery. 1983 Nov. 94(5):830-835.
[3] Weir GC. Lesser PB. Drop LJ. Fischer JE. Warshaw AL. The hypocalcemia of acute pancreatitis. Annals of Internal Medicine. 1975 Aug, 83(2):185-189.
[4] McMahon, M J. Woodhead, J S. Hayward, R D. The nature of hypocalcaemia in acute pancreatitis.British Journal of Surgery. 1978 Mar, 65(3):216-218.
[5] Imrie, C W. Beastall, G H.Allam, B F. O'Neil, J. Benjamin, I S. McKay, A J. Parathyroid hormone and calcium homeostasis in acute pancreatitis. British Journal of Surgery. 1978 Oct, 65(10):717-20.
[6] McMahon, M J. Heyburn, P J. Playforth, M J.Woodhead, J S.Parathyroid function during acute pancreatitis. British Journal of Surgery. 1982 Feb, 69(2):95-98.
[7]Usdin TB, Gruber C, Bonner TI.Identification and functional expression of a receptor selectively recognizing parathyroid hormone,the PTH2 receptor. J Biol Chem ,1995,270:15455–15458.
[8] Miles RR , Sluka J P , Halladay DL , et al . Parathyroid hormone(hPTH 1～38) the expression of UBP41 , an ubiquitin-specific protease , in bone . Cell Biochem , 2002 , 85 : 229.
[9] Lee K C , Brown D , Urena P , et al. Localization of PTHrP receptor mRNA in kidney.Am J Physiol , 1996 , 270 : 186–191.
[10] Seuwen K, Boddeke HG..Heparin-insensitive calcium release from intracellular stores triggered by the recombinant human parathyroid hormone receptor . B r J Pharmacol , 1995 , 114 : 1613.
[11] Tonn O , Kriegbaum S , Braitmaier A , et al . Expression-leveldependent activation of recombinant human parathyroid hormone/ parathyroid hormone - related peptide receptor : effect of human parathyroid hormone ( 1-34) (1-31) and ( 28-48) .Recept signal Transduct Res , 2000 , 20: 133.
[12]Chen Jing,Zhang Jian,Lin Shanyan. Down-regulation of PTH/PTHrP receptor in the kidney of patients with renal impairment. Chinese Medical Journal,1998 ,111:24–27.
[13]陈靖,顾勇,张健,朱蔚钰,林善炎.硝苯地平和罗钙全对尿毒症患者甲状旁腺素受体基因表达的影响.中华肾脏病杂志,2000,16.
[14]Michael L.Picton,Philip R.Moore,E.Barbara Mawer,David Houghton,Anthony J.Freemont,Alastair J.Hutchison,Ram Gokal,Judith A.Hoyland. Down-regulation of human osteoblast PTH/PTHrP receptor mRNA in end-stage renal failure.Kidney International, 2000 , 58 :1440～1449.
[15]Fang Qian,Albert Leung,Abdul Abou-Samra.Agonist-Dependent Phosphorylation of the Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor. Biochemistry,1998 ,37:6240–6246.
[16]Urena P,Lida-klein A,Kong XF et al.Regulation of the PTH/PTHrP receptor mRNA by glucocorticoids and PTH in ROS17/2.8 and OK cells.Endocrinology.1994,135.
[17]Urena P,Mannstadt M,Hruby M,Ferreira A,Schmitt F,Silve C,Ardaillou R Lacour B Abou-samra A B segre GV.Drueke T.Parathyroidectomy does not prevent the renal PTH/PTHrP receptor downregulation in uraemic rats.Kidney Int 1995,47:1797-1805.
[18] Miroslaw Smogorzewski,Jin Tian,Shaul G Massry . Down-regulation of PTH/PTHrP receptor of heart in CRF:Role of [Ca2 +]i . Kidney International, 1995, 47 :1182～1186 .
[19] Miroslaw Smogorzewski,Wojciech Marcinkowski,Guoxiang Zhang,Shaul G..Massry. Parathyroidectomy does not affect mRNA of PTH/PTHrP receptor in kidney,liver and heart.Am J Nephrol1997,17:187-192.
[20]Lin Y Xie,Albert Leung,Gino V Segre,Itsuo Yamamoto,Abdul B Abou-samra . Down regulation of the PTH/PTHrP receptor by vitamin D3 in the osteoblast-like ROS17/2.8 cells. The American Physiological Society, 1996 : 654-660.
[21]Katz, M. S., Gutierrez, G. E., Mundy, G. R., Hymer, T. K., Caulfield, M. P.,and McKee, R. L. Tumor necrosis factor and interleukin 1 inhibit parathyroid hormone-responsive adenylate cyclase in clonal osteoblast-like cells by downregulating parathyroid hormone receptors. Cell Physiol ,1992,153:206 –213.
[22]丁卫,马硕,杜国光.肿瘤坏死因子对大鼠成骨样细胞增殖与甲状旁腺素受体表达的影响.生物化学杂志,1997,13:39-42.
[23]E. TSUDA-FUTAMI, A. SHIOI, S. JONO, M. INABA, Y. NISHIZAWA, and H. MORII. Lymphoblastoid Interferon-a Downregulates Parathyroid Hormone (PTH)/PTH-related Peptide (PTHrP) Receptor Expression in Human Osteoblastic Cells (Saos-2). Bone,1998, 23:205–211.
[24]Tetsuya Kawane,Junsei Mimura,Yoshiaki Fujii-Kuriyama,Noboru Horiuchi.Identification of the promoter region of the parathyroid hormone receptor gene responsible for transcriptional suppression by insulin-like growth factor-I. Archives of Biochemistry and Biophysics,2005,439:61-69.
[25]Jongen J,Willemstein Van Hove E et al.Down regulation of the receptor for parathyroid hormone(PTH)and PTH-related peptide by transforming growth factor β in primary fetal rat osteoblasts.Endocrinology.1995,136:3260-3266.
[26]杜国光,李平风,杨非易,顾文霞.甲状腺素及维甲酸对成骨细胞甲状旁腺素受体的调节作用.生物化学杂志,1997,13.
[27]Celic S,Chilco PJ,Zajac JD,Makin TJ,Findlay DM.A type I collagen substrate increases PTH/PTHrP receptor expression and suppresses PTHrPmRNA expression in UMR 106-06 osteoblast like cells. Endocrinology. 1996,150:299-308.
[28] 黄鹤光,冷希圣.重症急性胰腺炎大鼠骨组织甲状旁腺素受体 mRNA 的表达.中华实验外科杂志,2001,18:303-304.
[29] 陆逢春,黄鹤光.重症急性胰腺炎肿瘤坏死因子-a 与低钙血症的关系.中华实验外科杂志,2005,22:397-398.
[30]周一农,黄鹤光.甲强龙对重症急性胰腺炎大鼠低钙血症和甲状旁腺激素受体表达的影响.中华实验外科杂志,2006,23:567-569.