谷子硒肥肥效试验研究
|
摘要
硒是人体正常生理过程中必需的微量元素之一。食物中的硒是人体获取硒元素的主要来源,而食物中的硒直接或者间接来源于土壤。由于土壤中的硒含量在地球表面上的分布极不均匀,一些地区严重缺硒(我国低硒地区约占国土面积的30%左右)。因此,通过施硒提高粮食中硒含量来满足人体正常生理过程中对硒的需求具有重要意义。本文采用田间试验和盆栽试验方法,进行了谷子富硒品种筛选、不同施硒方式和不同施硒时期等试验,研究了谷子品种富硒差异、施硒对谷子生长特性及籽粒品质的影响,以期为进一步开发富硒小米提供理论依据。
谷子富硒及优质性状品种筛选试验表明:不同品种籽粒硒含量存在着一定的差异,平均含硒量为0.075mg/kg,硒含量变幅为0.053～0.152 mg/kg;不同谷子品种的硒和蛋白质含量、产量存在较为丰富的遗传变异,以晋谷29蛋白质和硒含量最高,冀谷20表现出产量最高。
叶面施硒对谷子品种硒富集及品质影响试验结果表明:两种(60 g/hm2和120 g/hm2)叶面施硒处理,可使七个谷子品种的籽粒硒含量分别达到1.022～1.644 mg/kg、1.853～2.269 mg/kg,是未施硒的15.4、23.8倍,但对籽粒产量的影响无显著差异。叶面施硒增加多数试验品种籽粒中蛋白质含量;叶面喷施使冀谷22籽粒中可溶性糖和赖氨酸含量略有增加,使晋谷34籽粒中可溶性糖减少,对其他品种可溶性糖和赖氨酸含量的影响均无显著差异。
通过田间试验,研究了不同时期施硒对谷子富硒及品质的影响。结果表明:随着植株生长发育期的推进,施硒使三个谷子品种(本地谷、晋谷29、冀优2号)含硒量表现出相同的增加趋势:空白处理<拔节期<齐穗期<灌浆期。不同时期施硒(150g/hm2)处理可以提高籽粒中可溶性蛋白质的含量和部分品种中的蛋白质含量,后期施硒使籽粒中可溶性糖含量增加的几率及比例大于前期施硒,齐穗期施硒可以使籽粒中赖氨酸含量增加。
采用盆栽试验研究了谷子(冀优2号和晋谷34)施硒拌种对早期其硒富集及长势的影响。试验结果表明:随施硒量增加,两个品种地上部和地下部含硒量随之增加并呈显著相关。谷子根部硒含量明显大于地上部。适量拌种处理理促进植株的出苗、成活和干物重积累,超过一定拌种量则会抑制,但两个品种适宜量略有差异。
通过田间试验研究了不同施硒方式对谷子(冀优2号)硒富集分布、籽粒品质及叶绿素a荧光参数的影响。结果表明:施硒量(0-30g/hm2)与谷子籽粒中含硒量存在极显著相关。叶面施硒利用率明显高于拌种施硒。收获期空白处理各器官中硒浓度依次为:根>叶>茎>籽粒。收获期拌种处理各器官中硒浓度依次为:根>叶>籽粒>茎,随着施硒量的增加根部硒浓度比例明显增加。叶面施硒处理谷子体中硒浓度:叶>根>籽粒>茎,随着施硒量的增加叶中硒浓度比例明显增加。两种施硒方式对谷子产量没有显著性影响。叶面施硒方式有利于提高谷子光合效率,从而有助于提高籽粒品质。叶面施硒较拌种施硒更有利于提高籽粒中蛋白质、淀粉及赖氨酸含量。
Selenium is one of the essential trace elements which plays important roles in normal physiology of human body. Food which absorb selenium from the soil directly or indirectly, are main sources for human beings to get selenium. However, the distribution of selenium in the soil is extremely limited on the earth's surface. The lack of selenium is especially serious in some areas(For example,about 30% of the national territory of China are selenium shortage seriously). So to develop the Se-enriched food is very important for meeting the demand of selenium of the human body. In this paper, we do experiments in the following aspects: variety different Se-enriched of millet, different fertilizing methods of seleminu and different period application of selenium by field and pot methods, respectively, to explore the effect of Se-riched differences of millet, selenium on growth characteristics and grain quality, which will enrich the theory basis of exploiting Se-enriched millet.
The field experiment was carried out to screen of Se-enriched and High quality traits in millet. The result showed that Se contents in grains of the nine varieties of millet were appeared to be greatly diversified, the average contents of selenium was 0.075mg/kg and deviation range was 0.053～0.152 mg/kg. Moreover, protein contents and yield in millet landraces germplasms exhibited wide genetic diversity, showed that protein and selenium of Jingu29 was highest, the yield of JiGu 20 was the highest.
The field experiment was carried out to study the effects of foliar application of Na2SeO3 on contents of Se, protein, soluble sugar and lysine acid in grains. The results showed that the two treatments (60 g/hm2 and 120 g/hm2) of foliar application Na2SeO3 could increase Se contents in grains of the seven varieties of millet up to 1.022~1.644 mg/kg and 1.853~2.269 mg/kg, which were 15.4 and 23.8 times higher than those in control treatment respectively. Moreover, the grain yields of the seven varieties of millet showed negative correlation with foliar application of Na2SeO3. There was significant increase of protein contents in most the varieties of millets with foliar application of Na2SeO3. The soluble sugar and lysine acid in grains of Jigu22 were increased significantly by foliar application of Na2SeO3, but the soluble sugar of Jingu34 was reduced significantly, while there were no significant effects on the other varieties of millet. The field experiment was carried out to study the effects of different-period application of selenium on contents of Se and quality in grains of millet. The results showed that Se content of the three varieties of millet (Foxtail Millet, Jingu29,Jiyou.No.2) was increasing with the plants growing:CK< jointing stage < full heading stage < grain filling stage. There was increasing protein and soluble protein contents in grain of millets with different-period application of selenium(150g/hm2). The application of selenium in late stage would increase soluble sugar content more than early and application of selenium in full heading stage would increase lysine content in grain of millet.
The pot experiment of planting two cultivars (Jiyou.No.2 and Jingu 34) was carried out to study the effect of seed dressing with selenium on Se concents and growing of millet. The results showed that Se contents was increased with the increasing of selenium supply and was significantly correlated in shoot and root, roots was significantly higher than above ground parts of millet. Moreover, emergence, survival and dry matter accumulation of millet was increased with the proper level of selenium supply,but they were inhibited the high level of selenium and the two varieties of millet slightly difference.
A field experiment of was carried out to study the effect of different fertilizing methods of selenium on concentration and distribution, grain quality and the kinetics parameters of chlorophyll fluorescenc of millet (Jiyou.No.2). The results showed that Se content in grain was significantly related with the increase of selenium supply (0-30g/hm2). Effect of foliar application of selenium on Se content in grain was significantly higher than seed dressing with selenium. The distribution of Se content in organs of blank were root > leaf > stem>grain. The distribution of Se content in organs of seed dressing treatments were root>leaf> grain >stem, the proportion of Se concent in root increased significantly with the increase of selenium supply. The distribution of Se content in organs of spraying selenium treatments were leaf>root> grain >stem. The proportion of Selenium concent in leaf increased significantly with the increase of selenium supply. The two fertilizing methods of seleminu had no significant influence on the yield of the millet. foliar application of selenium improved photosynthetic efficiency of millet, which help to improve grain quality. Proteinand lysine contents in grain was increased the spraying selenium favorable than the seed dressing.
谷子富硒及优质性状品种筛选试验表明:不同品种籽粒硒含量存在着一定的差异,平均含硒量为0.075mg/kg,硒含量变幅为0.053～0.152 mg/kg;不同谷子品种的硒和蛋白质含量、产量存在较为丰富的遗传变异,以晋谷29蛋白质和硒含量最高,冀谷20表现出产量最高。
叶面施硒对谷子品种硒富集及品质影响试验结果表明:两种(60 g/hm2和120 g/hm2)叶面施硒处理,可使七个谷子品种的籽粒硒含量分别达到1.022～1.644 mg/kg、1.853～2.269 mg/kg,是未施硒的15.4、23.8倍,但对籽粒产量的影响无显著差异。叶面施硒增加多数试验品种籽粒中蛋白质含量;叶面喷施使冀谷22籽粒中可溶性糖和赖氨酸含量略有增加,使晋谷34籽粒中可溶性糖减少,对其他品种可溶性糖和赖氨酸含量的影响均无显著差异。
通过田间试验,研究了不同时期施硒对谷子富硒及品质的影响。结果表明:随着植株生长发育期的推进,施硒使三个谷子品种(本地谷、晋谷29、冀优2号)含硒量表现出相同的增加趋势:空白处理<拔节期<齐穗期<灌浆期。不同时期施硒(150g/hm2)处理可以提高籽粒中可溶性蛋白质的含量和部分品种中的蛋白质含量,后期施硒使籽粒中可溶性糖含量增加的几率及比例大于前期施硒,齐穗期施硒可以使籽粒中赖氨酸含量增加。
采用盆栽试验研究了谷子(冀优2号和晋谷34)施硒拌种对早期其硒富集及长势的影响。试验结果表明:随施硒量增加,两个品种地上部和地下部含硒量随之增加并呈显著相关。谷子根部硒含量明显大于地上部。适量拌种处理理促进植株的出苗、成活和干物重积累,超过一定拌种量则会抑制,但两个品种适宜量略有差异。
通过田间试验研究了不同施硒方式对谷子(冀优2号)硒富集分布、籽粒品质及叶绿素a荧光参数的影响。结果表明:施硒量(0-30g/hm2)与谷子籽粒中含硒量存在极显著相关。叶面施硒利用率明显高于拌种施硒。收获期空白处理各器官中硒浓度依次为:根>叶>茎>籽粒。收获期拌种处理各器官中硒浓度依次为:根>叶>籽粒>茎,随着施硒量的增加根部硒浓度比例明显增加。叶面施硒处理谷子体中硒浓度:叶>根>籽粒>茎,随着施硒量的增加叶中硒浓度比例明显增加。两种施硒方式对谷子产量没有显著性影响。叶面施硒方式有利于提高谷子光合效率,从而有助于提高籽粒品质。叶面施硒较拌种施硒更有利于提高籽粒中蛋白质、淀粉及赖氨酸含量。
Selenium is one of the essential trace elements which plays important roles in normal physiology of human body. Food which absorb selenium from the soil directly or indirectly, are main sources for human beings to get selenium. However, the distribution of selenium in the soil is extremely limited on the earth's surface. The lack of selenium is especially serious in some areas(For example,about 30% of the national territory of China are selenium shortage seriously). So to develop the Se-enriched food is very important for meeting the demand of selenium of the human body. In this paper, we do experiments in the following aspects: variety different Se-enriched of millet, different fertilizing methods of seleminu and different period application of selenium by field and pot methods, respectively, to explore the effect of Se-riched differences of millet, selenium on growth characteristics and grain quality, which will enrich the theory basis of exploiting Se-enriched millet.
The field experiment was carried out to screen of Se-enriched and High quality traits in millet. The result showed that Se contents in grains of the nine varieties of millet were appeared to be greatly diversified, the average contents of selenium was 0.075mg/kg and deviation range was 0.053～0.152 mg/kg. Moreover, protein contents and yield in millet landraces germplasms exhibited wide genetic diversity, showed that protein and selenium of Jingu29 was highest, the yield of JiGu 20 was the highest.
The field experiment was carried out to study the effects of foliar application of Na2SeO3 on contents of Se, protein, soluble sugar and lysine acid in grains. The results showed that the two treatments (60 g/hm2 and 120 g/hm2) of foliar application Na2SeO3 could increase Se contents in grains of the seven varieties of millet up to 1.022~1.644 mg/kg and 1.853~2.269 mg/kg, which were 15.4 and 23.8 times higher than those in control treatment respectively. Moreover, the grain yields of the seven varieties of millet showed negative correlation with foliar application of Na2SeO3. There was significant increase of protein contents in most the varieties of millets with foliar application of Na2SeO3. The soluble sugar and lysine acid in grains of Jigu22 were increased significantly by foliar application of Na2SeO3, but the soluble sugar of Jingu34 was reduced significantly, while there were no significant effects on the other varieties of millet. The field experiment was carried out to study the effects of different-period application of selenium on contents of Se and quality in grains of millet. The results showed that Se content of the three varieties of millet (Foxtail Millet, Jingu29,Jiyou.No.2) was increasing with the plants growing:CK< jointing stage < full heading stage < grain filling stage. There was increasing protein and soluble protein contents in grain of millets with different-period application of selenium(150g/hm2). The application of selenium in late stage would increase soluble sugar content more than early and application of selenium in full heading stage would increase lysine content in grain of millet.
The pot experiment of planting two cultivars (Jiyou.No.2 and Jingu 34) was carried out to study the effect of seed dressing with selenium on Se concents and growing of millet. The results showed that Se contents was increased with the increasing of selenium supply and was significantly correlated in shoot and root, roots was significantly higher than above ground parts of millet. Moreover, emergence, survival and dry matter accumulation of millet was increased with the proper level of selenium supply,but they were inhibited the high level of selenium and the two varieties of millet slightly difference.
A field experiment of was carried out to study the effect of different fertilizing methods of selenium on concentration and distribution, grain quality and the kinetics parameters of chlorophyll fluorescenc of millet (Jiyou.No.2). The results showed that Se content in grain was significantly related with the increase of selenium supply (0-30g/hm2). Effect of foliar application of selenium on Se content in grain was significantly higher than seed dressing with selenium. The distribution of Se content in organs of blank were root > leaf > stem>grain. The distribution of Se content in organs of seed dressing treatments were root>leaf> grain >stem, the proportion of Se concent in root increased significantly with the increase of selenium supply. The distribution of Se content in organs of spraying selenium treatments were leaf>root> grain >stem. The proportion of Selenium concent in leaf increased significantly with the increase of selenium supply. The two fertilizing methods of seleminu had no significant influence on the yield of the millet. foliar application of selenium improved photosynthetic efficiency of millet, which help to improve grain quality. Proteinand lysine contents in grain was increased the spraying selenium favorable than the seed dressing.
引文
[1] Rayman M P. Selenium in cancer prevention:Areview of the evidence and mecha- nism of act- ion[J].Proceedings of the Nutrition Soeiety, 2005, 64(4):527-542.
[2] Schwarz K, Foltz C M. Selenium as an integral part of factor 3 against dietary nec- roticliver degeneration [J].JAm.Chem.Soc.1957, 70(32):92-93.
[3] Rotruck J T, Pope A T, Ganther H E, etal.Selenium: biochemical role as a compo- nent of gluthathione peroxidase [J].Science, 1973, 179(5):88-90.
[4]杨光沂,周瑞华,朱莲珍,等.我国人民硒供给量标准的商榷微量元素[C],中国营养学会第四届全国学术会议论文摘要汇编,1985,(l-2):32.
[5] Mrak P, Desouza, Eahlytle A H, etal. Rate limiting steps in selenium Assimilation and volalita -tion by Indian mustar [J].Plant Physiol, 1998, 117:87-94.
[6]蒋彬.人体微量元素硒营养[J].广东微量元素科学,2002,9(2):1-6.
[7]张天锡.人体硒代谢与疾病[J].微量元素杂志,1989(3):1.
[8]陈历程,张勇.微量元素硒的研究现状及其食品强化[J].食品科学,2002,23(10):134-137.
[9]黄峙.食品硒源的生物学研究进展[J].食品科学,2001,22(5):90-94.
[10] Arthur J R, Beekett G J. Newer aspects of miero-nutrients in atrisk groups: new metabolicroles for selenium in Proe [J].Nutr.Soe, 1994, 53:615-624.
[11] Berry M J, Banu L, Larsen P R.Type 1 iodothyronine deiodinase is a selenoeys- teine containing enzyme [J].Nature, 1991, 349:438-440.
[12]孙邦复.硒对人体健康的重要作用及富硒食品的开发[J].适用技术市场,1992(l):6-8.
[13]胡秀丽,荣会,姜熙罗,等.硒对多不饱和脂肪酸负荷大鼠机体保护作用的研究[J].白求恩医科大学学报,2000,26(1):15-16.
[14]武瑞,仝宗喜,杨爽,等.硒对雏鸡血液及组织汞含量的影响[J]黑龙江畜牧兽医,2001(1): 3-5.
[15]刘会娟,姜龙,巩元芳,等.硒营养的研究进展[J].中国饲料添加剂,2006, (5):21.
[16]吴少平,熊世洲,刘藏.人工硒茶对铅毒性的拮抗作用.中国工业医学杂志,1998,11(1):15-18.
[17]吴永尧,卢向阳,彭振坤,等. Se在水稻中的生理生化作用探讨[J].中国农业科学, 2000,3(1): 100-103.
[18] Giovanelli J, Mudd S H, Datko A H. Sul-fur amino acids in plants in sulfur Amino in plants [M]. New York: Academic Press, 1980:453-505.
[19] Shrift A. Transport of selenate into Astragalus roots.Plant Physoil.1969, 44: 893-896.
[20]赵成义.土壤硒的生物有效性研究[J].中国环境科学,2004,24(2):184-187.
[21] Zayed A,Lytle C M,Terry N. Aeeumulation and volatilization of different chemical species of selenium by Plants.[J]planta,1998.206:284-292.
[22]迟凤琴.土壤环境中的硒和植物对硒的吸收转化[J].黑龙江农业科学,2001 (6):33-34.
[23] Fleming G A.Selenium in Irish soil and plants[J].Soil Science,1962,94:28-35.
[24]陆景陵.植物营养学(上册)[M].2版.北京:中国农业大学出版社,2003:124-128.
[25] Neuhierl B, Boeck A. On the mechanism of selenium tolerance in selenium accumulating plans Purification and characterization of a specific selenocysteine methytransferase from cultured cells of Astragalus bisulcatus [J]. Eur.J.Bioehem, 1996:239-238.
[26] Brown T A,Shrift A.Selenium:toxicitv and tolerance in higher plants [J]. Biol. Rev, 1982(57): 59-84.
[27]可成友,夏丽娜,吴晓芳,等.富硒方法在植物中的研究应用[J].现代中药研究与实践,2009, 23(5):79-82.
[28]罗盛国,徐宁彤,刘元英.叶面喷硒提高粮食中的硒含量[J].东北农业大学学报,1999,30(1): 18-22.
[29]张驰,吴永尧,彭振坤.植物硒的研究进展[J].湖北民族学院学报,2002,20(3):58-62.
[30]万洪富.生态环境中的硒及植物对它的吸收和转化[J].土壤学进展,1988,16(6):l-9.
[31]施和平,张英聚,刘振声.番茄对硒的吸收、分布和转化.植物学报[J],1993,35 (7):541-546.
[32]吴军,刘秀芳,徐汉生.硒在植物生命活动中的作用[J].植物生理学通讯,1999,35(5):417- 423.
[33]胡秋辉,杨方美,潘根兴,等.喷施硒对大豆品质和大豆食品硒水平的影响[J].中国油料作物学报,2001,23(3):42-45.
[34] Chen D, Ren S, Lu W. Effect of applying selenium fertilizer to improve soil and increase selenium level in food for prevention and treatment of Kaschin-Back disease.J Environ Sci,1993,5(3):299-309.
[35]中国农学会.全国首届青年农学学术年会论文集[C].北京:中国科学技术出版社,1992: 68-72.
[36]陈卫军,魏益民,张国权.国内外谷子的研究现状[J].杂粮作物,2000,20(3):27-29.
[37]张超,张晖,李冀新.小米的营养以及应用研究进展[J].中国粮油学报,2007,22(1):51-55.
[38] Nishizawa N. Proso millet protein elevates plasma level of high density- lipoprotein a new food function of proso millet [J].Biomedical and Environment Sciences, 1996, 9:209-212.
[39]宋东晓,高德成.小米的营养价值与产品开发[J].粮食加工,2005(1): 21-24.
[40]古世禄,刘厦.中国谷子蛋白质氨基酸组成的研究[J].华北农学报,1989,4(1):8-15.
[41]程汝宏.优质富硒谷子新品种冀优1号[J].中国种业,2002(7):38-39.
[42]赵海云,陈继富,王宏丽,等.优质富硒谷新品种晋谷29号选育及其小米深加工[J].作物研究, 2007(3):394-395.
[43] Antony U. The effect of fermentation on the primarynutrients in foxtail millet [J].Food Chemistry, 1996, 54(4):381-384.
[44]吴永尧,彭振坤,罗泽民.植物对硒的吸收及其效应[J].湖北民族学院学报,1997 (3):10-13.
[45]伊虎英,郝玉怀,鱼宏斌等.硒肥对低硒区谷子籽粒含硒量及产量的影响[J].土壤通报,1991 (1):49-50.
[46]周鑫斌,驰卫明,杨林章等.水稻子粒硒累积机制研究[J].植物营养与肥料学报,2008, 14(3): 503-507.
[47]布和敖斯尔,张东威,刘力,等.土壤硒区域分异及安全值的研究[J].土壤学报,1995,32 (2):186-192.
[48]程汝宏,刘正理.我国谷子育种目标的演变与发展趋势[J].河北农业科学,2003,27(增刊): 95-98.
[49]刘正理,孙世贤,程汝宏等.富铁营养保健型超早熟谷子新种质的创新[J].中国农业科学, 2006,39(5):1044-1048.
[50]张玲金,陈德勋,刘晓端等.植物中硒的分离和微波消解-原子荧光测定法[J].环境与健康杂志,2004,21(3):48-51.
[51]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:284 -287.
[52]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:295 -297
[53]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:302 -305
[54]张宪政等,作物生理研究法[M].北京:农业出版社,1992:150-157,191-212.
[55]程汝宏,刘正理.谷子育种中几个主要性状选育方法的探讨[J].华北农学报,2003.18(院庆专辑):145-149.
[56]刘三才,朱志华,李为喜等.谷子品种资源微量元素硒和蛋白质含量的测定与评价[J].中国农业科学,2009,42(11):3812-3818.
[57]王月福,于振文,李尚霞等.土壤肥力和施氮量对小麦氮素吸收运转及籽粒产量和蛋白质含量的影响[J].应用生态学报,2003,14(11):1868-1872.
[58]张磊,吴冬云,朱碧岩.灌浆期不同温光对水稻叶、籽粒可溶性蛋白质及可溶性糖动态变化的影响[J].华南师范大学学报,2002(2):98-101.
[59]周鑫斌,施卫明,杨林章.叶面喷硒对水稻籽粒硒富集及分布的影响[J].土壤学报2007,44(1):73-78.
[60]李志玉,郭庆元,徐巧珍等.不同大豆品种积累硒的特性及基因型差异[J].植物营养与肥料学报,2000,6(2):207-213.
[61]周勋波,吴海燕,谢甫绨.大豆喷施硒肥效应的初步研究[J].中国粮油学报,2005,20(1): 45-48.
[62]卫丽,王同朝,张桂兰.谷子品种资源营养品质分析及抗病性鉴定[J].华北农学报,1999, 14(2):1-5.
[63]吴永尧,罗泽民,彭振坤.水稻对硒的生物富集分布[J].湖南师范大学自然科学学报,1998,21 (4):76-79.
[64]陈少裕.膜脂过氧化对植物细胞的伤害[J].植物生理学通讯,1991,27(2):84-90.
[65]周勋波,吴海燕,王海英.喷施硒肥对大豆理化指标和品质的影响[J].中国粮油学报,2004, 19(5):38-42.
[66]张巽,王宏富.不同生育时期喷施硒肥对谷子籽粒含硒量的影响[J].安徽农学通报,2009,15 (14):85-86.
[67]王芳,林克惠.植物硒素营养的研究进展[J].云南农业大学学报,2004,19(4):417-422.
[68]尚庆茂,高丽红等.硒营养对水培莴苣品质的影响[J].中国农业大学学报, 1998,3(3):67-71.
[69]张艳玲,潘根兴,胡秋辉,等.叶面喷施Se肥对低Se土壤中大豆不同部位蛋白质组成及其硒分布的影响[J].南京农业大学学报,2003,26(1):37-40.
[70]张燕林,潘根兴.叶面施硒肥对低硒土壤中大豆不同蛋白质组分及其硒分布的影响[J].南京农业大学学报,2003,26(l):37-40.
[71] Peng X L,Liu Y Y,Luo S G,et al,2002.Effects of selenium on lipid peroxidation and oxidizing ability of rice roots under ferrous stress[J].Dong Bei Nong Ye Da Xue Xue Bao :Ying Wen Ban,9(1):9-15.
[72] Li J Y. Effect of applying selenium fertilizer to improve soil and increased selenium level of food on prevention and treatment of Kashin-Beck’s disease [J]. Chin J Endo, 1991(10):69-75.
[73]林匡飞,徐小清,郑利等.Se对小麦种子发芽与根伸长抑制的生态毒理效应[J].农业环境科学学报,2004,23(5),885-889.
[74]徐云,王子健,王文华等.Se和环境中富里酸对小麦种子发芽的影响及其生理特性[J].应用生态学报,1997,8(4):439-444.
[75] Trelease S F, Trelease H M. Physiological differentiation in Astragaluswith reference to selenium [J].Am JBot, 1939, 26:530-535.
[76] Trelease S F, Beath OA. Selenium, Its Geological Occurrence and Its Biological Effects in Relation to Botany Chemistry, Agriculture, Nutrition, and Medicine [M]. New York: Trelease &Beath. 1949.
[77]林世育,许春辉,张其德,等.叶绿素荧光动力学植物抗性生理学、生态学和农业现代化中的应用[J].植物学通报,1992,9(1):1-16.
[78]张其德,卢从明,刘丽娜,等.CO2倍增对不同基因型大豆光合色素含量和荧光诱导动力学参数的影响[J].植物学报,1997,39(10):946-950.
[79]康国芳,杨路华,李经伟.水分胁迫对小麦叶绿素a荧光参数的影响[J].湖北农业科学, 2008,47(1):22-24
[80] Stephen R C, Saville D J, Watkinson J H. The effects of sodium selenate applica- tions on growth and selenium concentration in wheat [J]. New Zealand Journal of Crop and Horti- cultural Science, 1989, 17(3):229-237.
[81]袁玲.烟草施用亚硒酸钠的研究[J].烟草科技,1994(6):33-35
[82]孙崇延,李德安,冯杰等.施加硒化肥对麦粒的化学元素及氨基酸含量的影响[J].微量元素与健康研究,1995,12(3):39-45.
[83] Bowler C,MontaguM V,inze SuPeroxide dismutase and stress tolerance[J].Annu Rev Plant Physiol Plantt Mol Biol,1992,43:83-116.
[2] Schwarz K, Foltz C M. Selenium as an integral part of factor 3 against dietary nec- roticliver degeneration [J].JAm.Chem.Soc.1957, 70(32):92-93.
[3] Rotruck J T, Pope A T, Ganther H E, etal.Selenium: biochemical role as a compo- nent of gluthathione peroxidase [J].Science, 1973, 179(5):88-90.
[4]杨光沂,周瑞华,朱莲珍,等.我国人民硒供给量标准的商榷微量元素[C],中国营养学会第四届全国学术会议论文摘要汇编,1985,(l-2):32.
[5] Mrak P, Desouza, Eahlytle A H, etal. Rate limiting steps in selenium Assimilation and volalita -tion by Indian mustar [J].Plant Physiol, 1998, 117:87-94.
[6]蒋彬.人体微量元素硒营养[J].广东微量元素科学,2002,9(2):1-6.
[7]张天锡.人体硒代谢与疾病[J].微量元素杂志,1989(3):1.
[8]陈历程,张勇.微量元素硒的研究现状及其食品强化[J].食品科学,2002,23(10):134-137.
[9]黄峙.食品硒源的生物学研究进展[J].食品科学,2001,22(5):90-94.
[10] Arthur J R, Beekett G J. Newer aspects of miero-nutrients in atrisk groups: new metabolicroles for selenium in Proe [J].Nutr.Soe, 1994, 53:615-624.
[11] Berry M J, Banu L, Larsen P R.Type 1 iodothyronine deiodinase is a selenoeys- teine containing enzyme [J].Nature, 1991, 349:438-440.
[12]孙邦复.硒对人体健康的重要作用及富硒食品的开发[J].适用技术市场,1992(l):6-8.
[13]胡秀丽,荣会,姜熙罗,等.硒对多不饱和脂肪酸负荷大鼠机体保护作用的研究[J].白求恩医科大学学报,2000,26(1):15-16.
[14]武瑞,仝宗喜,杨爽,等.硒对雏鸡血液及组织汞含量的影响[J]黑龙江畜牧兽医,2001(1): 3-5.
[15]刘会娟,姜龙,巩元芳,等.硒营养的研究进展[J].中国饲料添加剂,2006, (5):21.
[16]吴少平,熊世洲,刘藏.人工硒茶对铅毒性的拮抗作用.中国工业医学杂志,1998,11(1):15-18.
[17]吴永尧,卢向阳,彭振坤,等. Se在水稻中的生理生化作用探讨[J].中国农业科学, 2000,3(1): 100-103.
[18] Giovanelli J, Mudd S H, Datko A H. Sul-fur amino acids in plants in sulfur Amino in plants [M]. New York: Academic Press, 1980:453-505.
[19] Shrift A. Transport of selenate into Astragalus roots.Plant Physoil.1969, 44: 893-896.
[20]赵成义.土壤硒的生物有效性研究[J].中国环境科学,2004,24(2):184-187.
[21] Zayed A,Lytle C M,Terry N. Aeeumulation and volatilization of different chemical species of selenium by Plants.[J]planta,1998.206:284-292.
[22]迟凤琴.土壤环境中的硒和植物对硒的吸收转化[J].黑龙江农业科学,2001 (6):33-34.
[23] Fleming G A.Selenium in Irish soil and plants[J].Soil Science,1962,94:28-35.
[24]陆景陵.植物营养学(上册)[M].2版.北京:中国农业大学出版社,2003:124-128.
[25] Neuhierl B, Boeck A. On the mechanism of selenium tolerance in selenium accumulating plans Purification and characterization of a specific selenocysteine methytransferase from cultured cells of Astragalus bisulcatus [J]. Eur.J.Bioehem, 1996:239-238.
[26] Brown T A,Shrift A.Selenium:toxicitv and tolerance in higher plants [J]. Biol. Rev, 1982(57): 59-84.
[27]可成友,夏丽娜,吴晓芳,等.富硒方法在植物中的研究应用[J].现代中药研究与实践,2009, 23(5):79-82.
[28]罗盛国,徐宁彤,刘元英.叶面喷硒提高粮食中的硒含量[J].东北农业大学学报,1999,30(1): 18-22.
[29]张驰,吴永尧,彭振坤.植物硒的研究进展[J].湖北民族学院学报,2002,20(3):58-62.
[30]万洪富.生态环境中的硒及植物对它的吸收和转化[J].土壤学进展,1988,16(6):l-9.
[31]施和平,张英聚,刘振声.番茄对硒的吸收、分布和转化.植物学报[J],1993,35 (7):541-546.
[32]吴军,刘秀芳,徐汉生.硒在植物生命活动中的作用[J].植物生理学通讯,1999,35(5):417- 423.
[33]胡秋辉,杨方美,潘根兴,等.喷施硒对大豆品质和大豆食品硒水平的影响[J].中国油料作物学报,2001,23(3):42-45.
[34] Chen D, Ren S, Lu W. Effect of applying selenium fertilizer to improve soil and increase selenium level in food for prevention and treatment of Kaschin-Back disease.J Environ Sci,1993,5(3):299-309.
[35]中国农学会.全国首届青年农学学术年会论文集[C].北京:中国科学技术出版社,1992: 68-72.
[36]陈卫军,魏益民,张国权.国内外谷子的研究现状[J].杂粮作物,2000,20(3):27-29.
[37]张超,张晖,李冀新.小米的营养以及应用研究进展[J].中国粮油学报,2007,22(1):51-55.
[38] Nishizawa N. Proso millet protein elevates plasma level of high density- lipoprotein a new food function of proso millet [J].Biomedical and Environment Sciences, 1996, 9:209-212.
[39]宋东晓,高德成.小米的营养价值与产品开发[J].粮食加工,2005(1): 21-24.
[40]古世禄,刘厦.中国谷子蛋白质氨基酸组成的研究[J].华北农学报,1989,4(1):8-15.
[41]程汝宏.优质富硒谷子新品种冀优1号[J].中国种业,2002(7):38-39.
[42]赵海云,陈继富,王宏丽,等.优质富硒谷新品种晋谷29号选育及其小米深加工[J].作物研究, 2007(3):394-395.
[43] Antony U. The effect of fermentation on the primarynutrients in foxtail millet [J].Food Chemistry, 1996, 54(4):381-384.
[44]吴永尧,彭振坤,罗泽民.植物对硒的吸收及其效应[J].湖北民族学院学报,1997 (3):10-13.
[45]伊虎英,郝玉怀,鱼宏斌等.硒肥对低硒区谷子籽粒含硒量及产量的影响[J].土壤通报,1991 (1):49-50.
[46]周鑫斌,驰卫明,杨林章等.水稻子粒硒累积机制研究[J].植物营养与肥料学报,2008, 14(3): 503-507.
[47]布和敖斯尔,张东威,刘力,等.土壤硒区域分异及安全值的研究[J].土壤学报,1995,32 (2):186-192.
[48]程汝宏,刘正理.我国谷子育种目标的演变与发展趋势[J].河北农业科学,2003,27(增刊): 95-98.
[49]刘正理,孙世贤,程汝宏等.富铁营养保健型超早熟谷子新种质的创新[J].中国农业科学, 2006,39(5):1044-1048.
[50]张玲金,陈德勋,刘晓端等.植物中硒的分离和微波消解-原子荧光测定法[J].环境与健康杂志,2004,21(3):48-51.
[51]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:284 -287.
[52]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:295 -297
[53]鲍士旦等,土壤农化分析[M].中国农业出版社,2000:302 -305
[54]张宪政等,作物生理研究法[M].北京:农业出版社,1992:150-157,191-212.
[55]程汝宏,刘正理.谷子育种中几个主要性状选育方法的探讨[J].华北农学报,2003.18(院庆专辑):145-149.
[56]刘三才,朱志华,李为喜等.谷子品种资源微量元素硒和蛋白质含量的测定与评价[J].中国农业科学,2009,42(11):3812-3818.
[57]王月福,于振文,李尚霞等.土壤肥力和施氮量对小麦氮素吸收运转及籽粒产量和蛋白质含量的影响[J].应用生态学报,2003,14(11):1868-1872.
[58]张磊,吴冬云,朱碧岩.灌浆期不同温光对水稻叶、籽粒可溶性蛋白质及可溶性糖动态变化的影响[J].华南师范大学学报,2002(2):98-101.
[59]周鑫斌,施卫明,杨林章.叶面喷硒对水稻籽粒硒富集及分布的影响[J].土壤学报2007,44(1):73-78.
[60]李志玉,郭庆元,徐巧珍等.不同大豆品种积累硒的特性及基因型差异[J].植物营养与肥料学报,2000,6(2):207-213.
[61]周勋波,吴海燕,谢甫绨.大豆喷施硒肥效应的初步研究[J].中国粮油学报,2005,20(1): 45-48.
[62]卫丽,王同朝,张桂兰.谷子品种资源营养品质分析及抗病性鉴定[J].华北农学报,1999, 14(2):1-5.
[63]吴永尧,罗泽民,彭振坤.水稻对硒的生物富集分布[J].湖南师范大学自然科学学报,1998,21 (4):76-79.
[64]陈少裕.膜脂过氧化对植物细胞的伤害[J].植物生理学通讯,1991,27(2):84-90.
[65]周勋波,吴海燕,王海英.喷施硒肥对大豆理化指标和品质的影响[J].中国粮油学报,2004, 19(5):38-42.
[66]张巽,王宏富.不同生育时期喷施硒肥对谷子籽粒含硒量的影响[J].安徽农学通报,2009,15 (14):85-86.
[67]王芳,林克惠.植物硒素营养的研究进展[J].云南农业大学学报,2004,19(4):417-422.
[68]尚庆茂,高丽红等.硒营养对水培莴苣品质的影响[J].中国农业大学学报, 1998,3(3):67-71.
[69]张艳玲,潘根兴,胡秋辉,等.叶面喷施Se肥对低Se土壤中大豆不同部位蛋白质组成及其硒分布的影响[J].南京农业大学学报,2003,26(1):37-40.
[70]张燕林,潘根兴.叶面施硒肥对低硒土壤中大豆不同蛋白质组分及其硒分布的影响[J].南京农业大学学报,2003,26(l):37-40.
[71] Peng X L,Liu Y Y,Luo S G,et al,2002.Effects of selenium on lipid peroxidation and oxidizing ability of rice roots under ferrous stress[J].Dong Bei Nong Ye Da Xue Xue Bao :Ying Wen Ban,9(1):9-15.
[72] Li J Y. Effect of applying selenium fertilizer to improve soil and increased selenium level of food on prevention and treatment of Kashin-Beck’s disease [J]. Chin J Endo, 1991(10):69-75.
[73]林匡飞,徐小清,郑利等.Se对小麦种子发芽与根伸长抑制的生态毒理效应[J].农业环境科学学报,2004,23(5),885-889.
[74]徐云,王子健,王文华等.Se和环境中富里酸对小麦种子发芽的影响及其生理特性[J].应用生态学报,1997,8(4):439-444.
[75] Trelease S F, Trelease H M. Physiological differentiation in Astragaluswith reference to selenium [J].Am JBot, 1939, 26:530-535.
[76] Trelease S F, Beath OA. Selenium, Its Geological Occurrence and Its Biological Effects in Relation to Botany Chemistry, Agriculture, Nutrition, and Medicine [M]. New York: Trelease &Beath. 1949.
[77]林世育,许春辉,张其德,等.叶绿素荧光动力学植物抗性生理学、生态学和农业现代化中的应用[J].植物学通报,1992,9(1):1-16.
[78]张其德,卢从明,刘丽娜,等.CO2倍增对不同基因型大豆光合色素含量和荧光诱导动力学参数的影响[J].植物学报,1997,39(10):946-950.
[79]康国芳,杨路华,李经伟.水分胁迫对小麦叶绿素a荧光参数的影响[J].湖北农业科学, 2008,47(1):22-24
[80] Stephen R C, Saville D J, Watkinson J H. The effects of sodium selenate applica- tions on growth and selenium concentration in wheat [J]. New Zealand Journal of Crop and Horti- cultural Science, 1989, 17(3):229-237.
[81]袁玲.烟草施用亚硒酸钠的研究[J].烟草科技,1994(6):33-35
[82]孙崇延,李德安,冯杰等.施加硒化肥对麦粒的化学元素及氨基酸含量的影响[J].微量元素与健康研究,1995,12(3):39-45.
[83] Bowler C,MontaguM V,inze SuPeroxide dismutase and stress tolerance[J].Annu Rev Plant Physiol Plantt Mol Biol,1992,43:83-116.