雌二醇、孕酮对雌性绵羊生殖道β-防御素表达影响的研究
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摘要
内源性抗微生物肽是生物体内先天性免疫的重要组成成分,防御素(defensins)是其中一大家族。β-防御素是防御素家族中的重要成员之一,它主要分布于哺乳动物和鸟类的粘膜上皮组织内,被认为是黏膜表面抗微生物屏障的重要组成成分。关于生殖道β-防御素的研究主要涉及人类、小鼠;国内外学者对家畜,反刍动物生殖道防御素研究较少。本文应用电化学发光免疫法测定血清中雌二醇、孕酮的浓度;应用荧光定量RT-PCR法检测绵羊生殖道β-防御素(sBD-1)的相对表达量,就雌性绵羊生殖道sBD-1的表达与血清中雌二醇、孕酮含量之间的关系进行系统的研究。
结果显示:绵羊输卵管、子宫、宫颈、阴道4个器官内sBD-1的相对表达量差异显著,其中宫颈sBD-1的相对表达量明显高于阴道(P＜0.01),高于子宫和输卵管(P＜0.05);子宫sBD-1的相对表达量高于阴道(P＜0.05),但是与输卵管差异不显著(P＞0.05);输卵管sBD-1的相对表达量高于阴道(P＜0.05)。随发情时间的不同,同一器官内sBD-1的相对表达量呈规律性变化,发情周期各阶段差异显著(P＜0.05)。发情第六、九、十二天sBD-1的相对表达量明显高于发情当天(P＜0.01),高于发情第三天(P＜0.05),而发情第六、九、十二天之间sBD-1的相对表达量差异不显著(P＞0.05);发情第三天sBD-1的相对表达量高于发情当天(P＜0.05)。运用回归方程分析雌二醇及孕酮浓度与sBD-1的相对表达量变化的相关性,结果表明,雌二醇浓度与sBD-1的相对表达量的相关系数R~2=0.9146,孕酮浓度与sBD-1的相对表达量的相关系数R~2=0.9175,表明发情期雌二醇及孕酮浓度的变化均与sBD-1的相对表达量变化呈正相关。
Endogenous antimicrobial peptides are an important component in innate immunity of organism,and the defensins are a big family in endogenous antimicrobial peptides.β-defensins are one of important component of defensins family.β-defensins are proved to be a component of antimicrobial barrier and they distribute prominently in muscosal epithelial cells of mammalians and Aves.It is more research ofβ-defensins in human and mouse in the reproductive tract,and fewer in mammalians at present.In this study, Electro—chemiluminescence immunoassay was used to detect the content of estradiol and progesterone in serum.RT-PCR analyses revealed that sBD-1 is expressed in female sheep reproductive tract.We research the relation of expression ofβ-defensins in female reproductive tract with estradiol and progesterone in serum.
The results show,in the vagina,cervix,uterus,and fallopian tubes of sheep,the relation of expression of sBD-1 is different.The relation of expression of sBD-1 of cervix was significantly higher(P＜0.01)than that of vagina,and higher(P＜0.05)than that of uterus,and fallopian tubes.The relation of expression of sBD-1 of uterus was higher(P＜0.05)than that of vagina,but there is no difference between uterus and fallopian tubes(P＞0.05).The relation of expression of sBD-1 of fallopian tubes was higher(P＜0.05)than that of vagina.In different oestrus,the relation of expression of sBD-1 is difference in same tissue(P＜0.05),and show regularly change.The relation of expression of sBD-1 at day 6、9、12 is significantly higher(P＜0.01)than that of day 0, higher(P＜0.05)than that of day 3,but there is no difference between day 6 and day 9、12(P＞0.05).The relation of expression of sBD-1 at day 3 is higher(P＜0.05)than that of day 0.The relation of the expression of sBD-1 and the content of estradiol and progesterone was analyzed by recur equations.The result show that in different oestrus, the sBD-1 expression was not only positively correlated to the estradiol concentration (R~2=0.9146),but also to the progesterone concentration(R~2=0.9175).
结果显示:绵羊输卵管、子宫、宫颈、阴道4个器官内sBD-1的相对表达量差异显著,其中宫颈sBD-1的相对表达量明显高于阴道(P＜0.01),高于子宫和输卵管(P＜0.05);子宫sBD-1的相对表达量高于阴道(P＜0.05),但是与输卵管差异不显著(P＞0.05);输卵管sBD-1的相对表达量高于阴道(P＜0.05)。随发情时间的不同,同一器官内sBD-1的相对表达量呈规律性变化,发情周期各阶段差异显著(P＜0.05)。发情第六、九、十二天sBD-1的相对表达量明显高于发情当天(P＜0.01),高于发情第三天(P＜0.05),而发情第六、九、十二天之间sBD-1的相对表达量差异不显著(P＞0.05);发情第三天sBD-1的相对表达量高于发情当天(P＜0.05)。运用回归方程分析雌二醇及孕酮浓度与sBD-1的相对表达量变化的相关性,结果表明,雌二醇浓度与sBD-1的相对表达量的相关系数R~2=0.9146,孕酮浓度与sBD-1的相对表达量的相关系数R~2=0.9175,表明发情期雌二醇及孕酮浓度的变化均与sBD-1的相对表达量变化呈正相关。
Endogenous antimicrobial peptides are an important component in innate immunity of organism,and the defensins are a big family in endogenous antimicrobial peptides.β-defensins are one of important component of defensins family.β-defensins are proved to be a component of antimicrobial barrier and they distribute prominently in muscosal epithelial cells of mammalians and Aves.It is more research ofβ-defensins in human and mouse in the reproductive tract,and fewer in mammalians at present.In this study, Electro—chemiluminescence immunoassay was used to detect the content of estradiol and progesterone in serum.RT-PCR analyses revealed that sBD-1 is expressed in female sheep reproductive tract.We research the relation of expression ofβ-defensins in female reproductive tract with estradiol and progesterone in serum.
The results show,in the vagina,cervix,uterus,and fallopian tubes of sheep,the relation of expression of sBD-1 is different.The relation of expression of sBD-1 of cervix was significantly higher(P＜0.01)than that of vagina,and higher(P＜0.05)than that of uterus,and fallopian tubes.The relation of expression of sBD-1 of uterus was higher(P＜0.05)than that of vagina,but there is no difference between uterus and fallopian tubes(P＞0.05).The relation of expression of sBD-1 of fallopian tubes was higher(P＜0.05)than that of vagina.In different oestrus,the relation of expression of sBD-1 is difference in same tissue(P＜0.05),and show regularly change.The relation of expression of sBD-1 at day 6、9、12 is significantly higher(P＜0.01)than that of day 0, higher(P＜0.05)than that of day 3,but there is no difference between day 6 and day 9、12(P＞0.05).The relation of expression of sBD-1 at day 3 is higher(P＜0.05)than that of day 0.The relation of the expression of sBD-1 and the content of estradiol and progesterone was analyzed by recur equations.The result show that in different oestrus, the sBD-1 expression was not only positively correlated to the estradiol concentration (R~2=0.9146),but also to the progesterone concentration(R~2=0.9175).
引文
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