平菇汁对褐环粘盖牛肝菌生长影响的研究
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摘要
褐环粘盖牛肝菌是一种优良的外生菌根菌,且具有营养和药用价值,但在人工培养时生长缓慢、老化较快、产量较低,使得在实际生产应用中受到局限。为了加快该菌的生长速度,缩短生长周期,提高单位面积产量,也为探明该菌生长缓慢、老化快的原因,以及不同菌类生长差异和生长调控的机理,本文研究了平菇汁对褐环粘盖牛肝菌生长的促进作用。通过对平菇汁进行初步分离处理,分析了几种方法处理平菇汁对褐环粘盖牛肝菌生长的影响。结果表明,平菇汁对菌株SP9菌丝生长有明显的促进作用,在浓度为10-15%的条件下菌丝生长速度最快;用终浓度为75%的乙醇分离平菇汁所得到的乙醇分离产物,上清液和沉淀物对菌株SP9,SP6,SP7,XF1菌落直径的增长速度和菌丝干重具有极显著的促进作用,分别在上清液浓度为25%和沉淀物浓度为40%的条件下菌丝生长最好,且明显延缓了其老化速度;乙醇上清液和乙醇沉淀物对Suillus,96牛,00牛菌落直径的增长速度有抑制的作用,但显著增加了菌丝干重;室温条件下干燥得的乙醇沉淀物对菌丝生长的促进效果比高温条件干燥得的沉淀物好;乙醇上清液石油醚提取物对菌丝生长没有作用;乙醇上清液乙酸乙酯提取物对菌丝生长有促进作用;乙醇上清液混合溶剂提取物对菌丝生长有促进作用;乙醇沉淀物中粗多糖对菌丝生长有促进作用,硫酸铵盐析蛋白质对菌丝生长没影响;在低葡萄糖浓度的条件下,乙醇上清液浓度为10%时菌丝生长最快,乙醇沉淀物浓度为40%时菌丝生长最快;在高葡萄糖浓度的条件下,乙醇上清液对褐环粘盖牛肝菌生长的作用不受葡萄糖浓度的影响,乙醇上清液浓度为25%时菌丝生长最快,随着葡萄糖浓度的升高乙醇沉淀物的最适浓度变小,葡萄糖浓度为4.0g/100ml,乙醇沉淀物浓度为20%时菌丝生长最快;在低pH条件下,褐环粘盖牛肝菌在乙醇上清液浓度较低时生长较好,在高pH的条件下,褐环粘盖牛肝菌在上清液浓度较高时生长较好,乙醇沉淀物对菌丝生长的促进作用不受pH的影响。
Suillus luteus is a superior mycorrhiza fungi, and has nutritional and medicinal value. But because of its slow growth, rapid aging, low yield, its practical application is still limied. In order to promote the growth rate, shorten the growth cycle, improve the yield, also determine the reasons for slow growth, rapid aging of Suillus luteus, and growth difference between different fungi and growth regulation mechanism, in this paper, promotion of Pleurotus ostreatus juice to Suillus luteus was studied. Through the initial separation of Pleurotus ostreatus juice, analysis the influence of Pleurotus ostreatus juice on the mycelia growth of Suillus luteus in various treatment conditions. The result showed that, Pleurotus ostreatus juice has significant promotion to Suillus luteus ,when the concentration of Pleurotus ostreatus juice is 10-15%, the mycelia growth is the best. The ethanol supernatant and the ethanol precipitation promotes the growth rate and increases the colony diameter and the dry weight of strains SP9, SP6, SP7, XF1 extreme significantly, and delaies aged speed obviously. when the concentration of the ethanol supernatant is 25% and that of the ethanol precipitation is 40%, the mycelia growth is the best. The ethanol supernatant and the ethanol precipitation inhibites the enlarge rate of colony diameter of strains Suillus, 96-Niu, 00-Niu, but significantly increases their dry weight. The promoting effect to Suillus luteus of the ethanol supernatant dried at room temperature is better than which dried at room temperature. The ethanol supernatant extracts obtained by ethyl acetate and that obtained by mixed solvent promote the growth of Suillus luteus. Crude polysaccharide promotes the growth of Suillus luteus, ammonium sulfate protein has no influence. Under low glucose concentration condition, when the concentration of the ethanol supernatant is 10% and that of the ethanol precipitation is 40%, the mycelia growth is the best; under high glucose concentration conditions, the effect of the ethanol supernatant to the mycelia growth have no relation with glucose concentration, the optimum concentration of the ethanol supernatant is 25%; as the glucose concentration increased the optimal concentration of ethanol precipitation become smaller gradually, when glucose concentration is 4.0g/100ml, the optimal concentration of ethanol precipitation is 20%. Under low pH conditions, the mycelia grow well when the ethanol supernatant concentration is lower; under high pH conditions, the mycelia grow well when the ethanol supernatant concentration is higher; the effect of the ethanol precipitation to the mycelia growth have no relation with pH.
Suillus luteus is a superior mycorrhiza fungi, and has nutritional and medicinal value. But because of its slow growth, rapid aging, low yield, its practical application is still limied. In order to promote the growth rate, shorten the growth cycle, improve the yield, also determine the reasons for slow growth, rapid aging of Suillus luteus, and growth difference between different fungi and growth regulation mechanism, in this paper, promotion of Pleurotus ostreatus juice to Suillus luteus was studied. Through the initial separation of Pleurotus ostreatus juice, analysis the influence of Pleurotus ostreatus juice on the mycelia growth of Suillus luteus in various treatment conditions. The result showed that, Pleurotus ostreatus juice has significant promotion to Suillus luteus ,when the concentration of Pleurotus ostreatus juice is 10-15%, the mycelia growth is the best. The ethanol supernatant and the ethanol precipitation promotes the growth rate and increases the colony diameter and the dry weight of strains SP9, SP6, SP7, XF1 extreme significantly, and delaies aged speed obviously. when the concentration of the ethanol supernatant is 25% and that of the ethanol precipitation is 40%, the mycelia growth is the best. The ethanol supernatant and the ethanol precipitation inhibites the enlarge rate of colony diameter of strains Suillus, 96-Niu, 00-Niu, but significantly increases their dry weight. The promoting effect to Suillus luteus of the ethanol supernatant dried at room temperature is better than which dried at room temperature. The ethanol supernatant extracts obtained by ethyl acetate and that obtained by mixed solvent promote the growth of Suillus luteus. Crude polysaccharide promotes the growth of Suillus luteus, ammonium sulfate protein has no influence. Under low glucose concentration condition, when the concentration of the ethanol supernatant is 10% and that of the ethanol precipitation is 40%, the mycelia growth is the best; under high glucose concentration conditions, the effect of the ethanol supernatant to the mycelia growth have no relation with glucose concentration, the optimum concentration of the ethanol supernatant is 25%; as the glucose concentration increased the optimal concentration of ethanol precipitation become smaller gradually, when glucose concentration is 4.0g/100ml, the optimal concentration of ethanol precipitation is 20%. Under low pH conditions, the mycelia grow well when the ethanol supernatant concentration is lower; under high pH conditions, the mycelia grow well when the ethanol supernatant concentration is higher; the effect of the ethanol precipitation to the mycelia growth have no relation with pH.
引文
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