摘要
分布在鲁中南泰沂山区的沂蒙黑山羊是山东省优良的地方品种,该品种黑山羊以耐粗饲、抗病能力强、绒毛质量好、肉质鲜美而著称,属肉、皮、绒兼用型品种。本实验以沂蒙黑山羊为研究对象,就性周期外周血中卵泡刺激素(FSH)、黄体生成素(LH)的分泌变化、性周期不同阶段促性腺激素受体免疫反应阳性细胞在输卵管不同部位中的位置分布以及卵泡刺激素受体(FHSR)mRNA、黄体生成素受体(LHR)mRNA在输卵管不同部位表达量的差异进行了研究,结果如下:
1.应用放射免疫测定法(RIA)对沂蒙黑山羊在性周期各阶段外周血中FSH、LH的含量进行了测定。结果表明:外周血FSH、LH含量的变化趋势相似,其基本走势为由发情前期到发情期降到最低,之后又有所升高。FSH含量在发情前期最高(1.6067±0.09955mIU/ml),与其它三个时期之间差异显著(0.01
0.05)。
2.运用免疫组织化学方法研究了性周期中促性腺激素受体免疫阳性细胞在输卵管的分布情况。FSHR免疫阳性细胞、LHR免疫阳性细胞在性周期不同阶段的输卵管各部位中都有存在,主要存在于输卵管的黏膜上皮细胞、固有层细胞以及血管内皮细胞中。
3.利用RT-PCR技术,以GAPDH为内参基因,研究了沂蒙黑山羊性周期中FSHR mRNA、LHR mRNA在输卵管的表达差异。结果显示:性周期中FSHR mRNA、LHR mRNA在输卵管中都有表达。FSHR mRNA在漏斗部以间情期的表达量最高,发情期最低,发情期与其它三个时期之间差异极显著(P<0.01);壶腹部在间情期的表达量最高,与其它三个时期差异极显著(P<0.01),在发情前期表达量最低。在峡部则是发情后期表达量最高,发情前期最低,发情前期与其它三个时期差异极显著(P<0.01)。LHR mRNA表达量与FSHR mRNA呈相反规律,漏斗部LHR mRNA表达量在间情期最低,发情期最高,且发情期与其它三个时期之间差异极显著(P<0.01);壶腹部则是发情前期表达量最高,间情期最低,发情前期与其它三个时期之间差异极显著(P<0.01);峡部在发情前期表达量最高,发情后期最低,发情前期与其它三个时期之间差异均达极显著水平(P<0.01)。
YiMeng Black Goat from Tai-Yi mountain areas is one of the best local breeds of ShanDong, which is well known for gaining well on the roughest forage, strong inhibitory effect, high quality down and delectable flesh. YiMeng Black goat was investigated in our study for the change of FSH and LH secretion in peripheral blood during estrous cycle, distribution of GTH receptors positive cells in different phases of estrous cycle and the expression of FSHR and LHR in different parts of oviduct. The results showed that:
1. RIA (radioimmunoassay) was used to assay the concentration of FSH and LH in peripheral blood during every phases of estrous cycle. The results suggested that changes of FSH and LH concentration in peripheral blood were similar. The tend of content of GTH hormone decide to the minimum from proestrum to estrus and then rise again. Concentration of FSH in peripheral blood was highest in proestrum (1.6067±0.09955mIU/ml), which is significantly different from other three phases (0.01
0.05).
2. Immunohistochemical method was utilized to study the distribution of GTH receptors positive cells in oviduct during estrous cycle. FSHR positive cells and LHR positive cells were found in different parts of oviduct in different phases of estrous cycle. The majority of those positive cells were found in oviductal mucosa, lamina propria and vascular endothelial cells.
3. RT-PCR method with GAPDH as house-keeping gene was used for different expression of FSHR mRNA and LHR mRNA in oviduct during estrous cycle. The results indicated that FSHR mRNA and LHR mRNA were expressed in oviduct during estrous cycle. In infundibularis partes, the expression of FSHR mRNA was highest in diestrum and lowest in estrum. The expression of estrum has significantly different with other three phases (P<0.01). In ampulla, expression of FHSR mRNA was highest in diestrum, which is significantly different with other three phases (P<0.01); and lowest in proestrum. In isthmus the expression level of FHSR mRNA was highest in metaestrum and lowest in proestrum which is significantly different with other three phases (P<0.01). The expression of LHR mRNA is contrary to the expression of FSHR mRNA. In infundibularis partes, LHR mRNA reached the lowest level in diestrum and was highest in estrum which is significantly different with other three phases(P<0.01); In ampulla expression was highest in proestrum and lowest in diestrum, the expression in estrum has significantly different with other three phases(P<0.01); In isthmus the expression was highest in proestrum which has significantly different with other phases (P<0.01)and lowest in metaestrum.
引文
成令忠.现代组织学[M].上海:上海科学技术文献出版社,2007:532-537.
成令忠.现代组织学[M].上海:上海科学技术文献出版社,2007:1020-1021.
程玉,胡志强.腺垂体发育调控分子的研究进展[J].国外医学:遗传分册,2005,28(1):33-37.
初晨宇,吕葆真,孙岚等.卵泡刺激素及其受体在大鼠胰腺的共定位观察[J].解剖学报,2005,36(3):303-305.
崔胜,夏国良.绵羊垂体促性腺激素细胞的细胞类型以及在发情周期内的变化[J].中国农业大学报,1998,3(5):114-118.
崔胜,张建超,曹兴元等.绵羊妊娠期胎儿垂体促性腺激素细胞的发生发育与血浆B-LH浓度的变化关系[J].中国农业大学学报,2003,8(1):95-98.
丰培金,牛淑玲,周虚.促卵泡素受体表达与调节研究进展[J].中国畜牧兽医,2004,31(2):17-19.
高立坤,葛仕豪,王树迎等.不同繁殖力山羊年周期中FSH和LH分泌规律的研究[J].畜牧兽医学报,2008,39(1):37-41.
葛仕豪,高立坤,侯衍猛等.济宁青山羊发情周期内促性腺激素和性腺激素分泌规律的研究[J].西南农业学报,2007,20(6):1348-1352.
郝建明,刘平,张平等.黄体生成素和卵泡刺激素在大鼠垂体前叶的细胞共定位研究[J].解剖学报,1997,28(1):53.
侯放亮.牛繁殖与改良新技术[M].北京:中国农业出版社,2005:15-16.
侯衍猛,曹洪防,徐云华等.莱芜黑山羊发情周期中FSH、LH、E2和P的分泌规律[J].中国兽医学报,2006,26(3):340-343.
焦淑贤,王瑞祥,蔡正华等.枫泾和长白青年母猪首次发情周期内血清中5种生殖激素的变化[J].畜牧兽医学报,1993,23(3):202-206.
焦淑贤,王瑞祥,蔡正华等.枫泾和长白母猪发情期促卵泡素、促黄体素含量动态变化研究[J].中国农业科学,1992,25(6):80-85.
姜怀志,李向军,戢爽等.辽宁绒山羊母羊FSH全年分泌规律的研究[J].中国畜牧杂志,2008,44(15):15-17.
金光明,杨倩,王珏等.猪雌性生殖道黏膜显微和超微结构观察[J].畜牧兽医学报,2005,36(6): 612-615.
金海燕.输卵管的结构与功能研究进展[J].重庆医学,2006,35(18):1716-1718.
李金林,樊新忠.沂蒙黑山羊繁殖规律调查报告[J].山东畜牧兽医,1993,2:12-14.
李俊杰,贾青,张正珊.雌性哺乳动物促卵泡素受体与促黄体素受体的结构与表达[J].生命的化学,2003,23(4):289-291.
凌秀凤,李红霞.黄体生成素与辅助生殖[J].国外医学妇幼保健分册,2003,14(5):285-288.
刘爱军,罗善云,孟运莲.大鼠去卵巢前后血清雌二醇浓度变化及其对输卵管粘膜上皮的影响[J].解剖学报,2000,31(2):164-166.
刘嘉茵,Bruce J.A ronow, David Wittee,et al.猪卵巢周期中卵泡刺激素和黄体生成素受体mRNA的定位及表达[J].生殖医学杂志,1998,7(2):92-97.
刘俊伟,安志兴,余燕等.绵羊发情期输卵管组织结构的观察[J].贵州农业科学,2009,37(10):121-123.
刘润兰,邝国壁.人育龄期输卵管粘膜上皮表面结构周期性变化的扫描电镜观察[J].解剖学报,1989,20(3):273-277.
罗兰.女性生殖生理学基础.Chinese journal of andrology,2000,14(4):274-277.
罗丽兰.输卵管的解剖和功能[J].中国实用妇科与产科杂志,2000,16(4):213-214.
罗文祥,祝诚,吴燕婉.IGF-I及其受体、IGF结合蛋白-2和LH受体mRNA在卵泡中的表达[J].动物学报,1999,4(45):427-434.
任莹,邰发道.促性腺激素的神经内分泌调控[J].现代生物医学进展,2006,6(9):82-85.
桑润滋.动物繁殖生物技术[M].北京:中国农业出版社,2002.
沈霞芬.家畜组织学与胚胎学[M].北京:中国农业出版社,2005:209-210.
石长青,安铁洙,邹啸环.繁殖季节蒙古母马垂体促性腺激素细胞分泌颗粒与血清FSH、LH浓度的相关性[J].中国兽医杂志,2006,42(6):15-17.
谭娟,余四九,崔燕.妊娠期牦牛输卵管的组织结构观察[J].中国兽医科学,2008,38(07):622-625.
王天有,陈永耀,尚伟杰等.绵羊输卵管组织结构的观察[J].贵州农业科学,2006,34(6):13-15.
王元兴,郎介金.动物繁殖学[M].江苏科学技术出版社,1997.
吴馨培,王树迎.莱芜黑山羊卵巢FSHr、LHr的免疫组化定位研究[J].生命科学研究,2006,10(4):367-371.
吴馨培,王树迎.小鼠卵巢促性腺激素受体免疫组化的定位[J].中国实验动物学报,2007,15(1):52-55.
项晓人,朱永泽.动情期家兔输卵管上皮及其分泌活动的电镜观察[J].电子显微学报,1994,3:168-172.
谢碧文,王志坚,张耀光.鱼类垂体发育研究进展[J].水生生物学报,2008,32(1):102-108.
谢炳坤.广西黄牛和水牛发情周期血清生殖激素变化规律的研究[M].广西大学,2005.
谢成侠.家畜繁殖原理(第一版)[M].江苏科技出版社,1983:65-143.
谢衷明,毛全福,徐美红.雌性大鼠离体垂体前叶内在促黄体生成激素释放节律性现象[J].生理学报,1997,49(6):649-656.
邢晋祎,蔡文学,吕金光.沂蒙黑山羊生产现状及发展对策[J].四川草业,2005,2:55-57.
徐直,蒋振国,叙一树等.黑白花奶牛发情周期血液中促卵泡素、促黄体素、促乳素、孕酮、雌二醇的含量变化规律[J].华北农学报,1996,11(3):121-127.
杨利国.动物繁殖学[M].北京:中国农业出版社,2003:49.
于辉,唐旭,吕葆真等.卵泡刺激素受体在大鼠下颌下腺及胃的定位和分布研究[J].解剖学报,2006,37(3):337-339.
于一.动情周期中小白鼠生殖系统的组织化学观察Ⅲ.输卵管[J].解剖学报,1959,(4):1-2.
余燕,陈永耀,王天有等.黄牛输卵管结构的组织学观察[J].中国兽医科学,2007,37(02):167-170.
张森,谭建华.大鼠发情周期中主要生殖激素的变化[J].动物医学进展,2005,26(12):1-6.
章孝荣,王建辰.山羊GnRH和促性腺激素的释放特点[J].中国兽医学报,1997,17(2):177-179.
张英杰,刘月琴,储明星.小尾寒羊高繁殖力和常年发情内分泌机理的研究[J].畜牧兽医学报,2001,32(6):510-516.
张忠诚主编.家畜繁殖学(第三版)[M].北京:中国农业出版社,2000.
周虚,董伟,庄临之.猪垂体细胞单层培养[J].黑龙江动物繁殖,2001,9(2):4-5.
郑亦辉,张德福,马恒东.湖羊和美利奴羊发情期外周血浆中促性腺激素脉冲分泌的差异[J].中国畜牧杂志,1991,27(2):23-24.
周寿康,毛全福,赵伟等.雌性大鼠动情周期中黄体生成素细胞形态变化的图像分析[J].解剖学报,1996,27(4):423-428.
邹仲之.组织学与胚胎学[M].北京:人民卫生出版社,2004:205.
Abdennebi L, Monget P, Pisselet C, et al. Comparative expression of luteinizing hormone and follicle-stimulating hormone receptors in ovarian follicles from high and low prolific sheep breeds [J]. Biology of Reproduction, 1999, 60:845-854.
Abe H, Oikawa T. Regional variations in the ultrastructural features of secretory cells in the rat oviductal epithelium [J]. The Anatomical Record, 1994, 240:77-85.
Allen E. The oestrous cycle in the mouse[J]. American Journal of Anatomy, 2005,30: 297-371.
Andersen CY. Characteristics of human follicular fluid associated with sueeessful conception after in vitro fertilization [J]. Clin Endocrinol Metab, 1993, 77:1227-1234.
Arjamaa O, Talo A.Smooth muscle of the quail oviduct functions as a stretch receptor during ovum transport [J]. Biol Reprod, 1983, 29:472-478.
Alireza Fazeli. Sperm-ociduct induction of capacitation and preferential binding of oncapacitated spermatozoa to oviductal epithelial cells in porcine species[J]. Biol Reprod, 1999, 60: 879-886.
Bao B, Garverick H A. Expression of steroidogenic enzyme and gonadotropin receptor genes in bovine follicles during ovarian follicular waves: a review [J]. Journal of Animal Scince, 1998, 76: 1903-1921.
Bao B, Garverick H A, Smith GW,et al. Expression of messenger ribonucleic acid (mRNA) encoding 3 beta- hydroxysteroid dehydrogenase delta 4, delta 5 isomerase (3 beta-HSD) during recruitment and selection of bovine ovarian follicles: identificationof dominant follicles by expression of 3 beta-HSD mRNA within the granulosa cell layer[J]. Biology of Reproduction, 1997, 56: 1466-1473.
Barboni B, Mattioli M, Seren E. Influence of progesterone on boar sperm capacitation [J]. Endocrinol, 1995, 144(1):13-18.
Barnes BG. Electron microscope studies on the secretory cytology of the mouse anterior pituitary [J]. Endocrinology, 1962, 71(4):618.
Bastings E, Beckers A, Reznik M, et al. Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone cells in separate cells in the bovine[J]. Biol Reprod, 1991, 45(5):788.
Bathla H, Guraya S S, Sangha G K. Role of estradiol in the capacitation and acrosome reaction of hamster epididymal spermatozoa in the isolated uterus of mice incubated in vitro [J]. Indian J Pharmacol, 1999, 43(2):211-217.
Bertrand E, Van-den-Bergh M, Englert Y. Clinical parameters influencing human zona pellucida thickness [J]. Fertil Steril, 1996, 66:408-411.
Botero-Ruiz W, Laufer N, DeCherney AH, et al. The relationship between follicular fluid steroid concentration and successful fertilization of human oocytes in vitro[J]. Fertil Steril, 1984, 41:820-826.
Brann D W, Mahesh V B. Excitatory amino acids: evidence for a role in the control of reproduction and anterior pituitary hormone secretion [J]. Endocr Rev, 1997, 18:678-700.
Brenner R M, Carlisle KS, Hess D L, et al. Morphology of the oviducts and endometria of cynomolgus macaques during the menstrual cycl[J]. Biology of Reproduction, 1983, 29:1289-1302.
Brinkley HJ. Endocrine signalling and female reproduction[J].Biol Reprod,1981,24:22-43.
Buhi W C, Vallet J L, Bazer F W. De novo synthesis and release of polypeptides from cyclic and early pregnant porcine oviductal tissue in explant culture [J]. Exp Zool, 1989, 252:79-88.
Camp T A, Rahal J O, Mayo K E. Cellular localization and hormonal regulation of follicle-stimulating hormone and luteinizing hormonr receptor messenger RNAs in the rat ovary [J]. Mol Endocrinol, 1991, 5:1405-1417.
Cara J E, Rosenfield R L. Insulin-like growth factor Iand insulin potentiate luteinizing hormone-induced androgen synthesis by rat ovarian thecal-interstitial cell[J]. Endocrinology, 1988, 123:733-741.
Carlson H E, Kane P, Lei Z M, et al. Presence of luteinizing hormone/human chorionic gonadotropin receptors in male breast tissues[J]. Clin Endocrinol Metab, 2004, 89(8): 4119-4123.
Chitra Lekha Dahia, Jagannadha A, Rao. Demonstration of Follicle-Stimulating Hormone Receptor in Cauda Epididymis of Rat[J]. Biol Reprod, 2006, 75:98-106.
Clement C C, Robert H L. Pituitary development and physiology[J]. Pituitary, 2007, 10: 335-350.
Dacheux F. Heterogeneity in the subcellular localization of LH and FSH in the LH/FSH cells [J]. IRCS Med Sci, 1986, 14:215.
Dasen J S, Rosenfeld M G. Signaling mechanisms in pituitary morphogenesis and cell fate determination [J]. Current Opinion in Cell Biology, 1999, 11:669-677.
Deimann H D, Brown E M. Textbook of Veterinary Histology[M]. Philadelphia: LEA & FEBIGER, 1981:322-324.
Demott R P, Lefebvre R, Suarez S S. Carbohydrates mediate the adherence of hamster sperm to oviductal epithelium [J]. Biol Reprod, 1995, 52:1395-1403.
Demott R P, Suarez S S. Hyperactivated sperm progress in the mouse oviduct [J]. Biol Reprod, 1992, 46:779-785.
Derecka K, Pietila EM, Rajaniemi HJ, et al. Cycle dependent LH/hCG receptor gene expression in porcine nongonadal reproductive tissue[J]. Physiol Pharmacol, 1995, 46: 77-85.
Dierschke D J, Bhttacharya A N, Atkinson L E, et a1. Choral oscillations of plasma LH levels in the ovariectomized rhesus monkey[J]. Endocrinology, 1970, 87:850-853.
Dobson H. Plasma gonadotrophins and estradiolduring oestrus in the cow[J]. Reprod. Fert, 1978, 52:51-53.
Dunkel L, Tilly J L, Shikone T, et al. Follicle-stimulating hormone receptor expression in the rat ovary: Increase during prepubertal development and regulation by the opposing actions of transforming growth factorsβandα[J]. Biol Reprod, 1994, 50:940-948.
Ellington J E, Ball B A, Yang X. Binding of stallion spermatozoa to the equine zona pellucida after coculture with oviductal epithelial cells [J]. Reprod Fertil, 1993, 98:203-208.
Eriksen T, Terkelsen O, Hyttel P, et al. Ultrastructural features of secretory cells in the bovine oviduct epithelium [J].Anat Embryol, 1994, 190(6):583-590.
Espinasse PG. The oviductal epithelium of the mouse[J]. Am.J.Anat, 1935, 69: 363-368.
Farnworth P G. Gonadotropin secretion revisited. How many ways can FSH leave a gonadotroph?[J]. J Endocrinol, 1995, 145:387-395.
Fortune J.E. Ovarian follicular growth and development in mammals[J].Biol. Reprod, 1994, 50:225-232.
Fu JF, Huang WQ , Wang ShE, et al. Studies of distribution of FSH, LH and colocalization with GnRHR in rat submaxillary grand[J]. ActaAnatomica Sinica, 2004, 35(6): 652-655.
Fukuda A, Mori C, Hashimoto H, et al. Effects of prolactin during preincubation of mouse spermatozoa on fertilizing capadity in vitro[J]. Vitro Fert Embryo Tranf, 1989, 6(2):92-97.
Furuya S, Endo Y, ObaM, et al. Effect of epidermal growth factor (EGF) on mouse sperm capacitation[J]. Nippon Sanka Fujinkai Zasshi, 1993, 45(11):1313-1319.
Gandolfi F, Moor RM. Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells [J]. Reprod Fertil, 1987, 81:23-28.
Gawronska B, Paukku T, Huhtaniemi I, et al. Oestrogen- dependent expression of LH/ hCG receptors in pig Fallopian tube and their role in relaxation of the oviduct [J]. Reproduction and Fertility,1999, 115:293- 301.
Gawronska B, Stepien A, Ziecik A J. Effect of estradiol and progesterone on oviductal LH-receptors and LH-dependent relaxation of the porcineoviduct[J]. Theriogenology, 2000a, 53:659-672.
Gawronska B, Stepien A, Ziecik A J. Role of luteinizing hormone in control of oviduct function [J]. Reprod Dom Anim, 2000b, 35:129-133.
Geisert R D, Buhi W C, Fields M. Synthesis and release of polypeptides by bovine oviductal tissue in culture [J]. Biol Reprod, 1987, 36(11):84.
Girod C, Dubois P M, Trouillas J, et al. Immunohistochemical localization of FSH and LH in the pars distalis of Vervet (Cercopithecus aethiops) and Baboon (Papio hamadryas) pituitaries[J]. Cell Tissue Res, 1981, 217(2):245.
Gordts S, Campd R, Rombants L, et al.Endoscopic visualization of the process of the process of fimbrial ovum retrieval in the human [J].Hum Reprod, 1998, 13(6):1425.
Goxe B, Prunier A, Remy J J, et al. Ontogeny of gonadal luteinizing hormone and follicle-stimulating hormone receptors in gonadotropin and testosterone secretion[J]. Biol Reprod, 1993, 49:609-616.
Green C M, Cockle S M, Watson P F, et al. Fertilization promoting peptide, a tripaptide similar to thyrotrophin-releasing hormone, stimulates the capacitation and fertilizing ability of human spermatozoa in vitro[J]. Hum Reprod, 1996, 11(4):830-836.
Grippo A A, Way A L, Killian G J. Effect of bovine ampullary and isthmic oviductal fluid on motility, acrosome reaction and fertility of bull spermatozoa[J]. Journal of Reproduction and Fertility, 1995, 105:57-64.
Gudermann T, Bimbaumer M, Birnbaumer L. Evidence fordual coupling of the murine luteinizing hormone receptor to adenylyl cyclase and phosphoinositide breakdown and Ca~(2+) mobilization[J]. Biol Chem, 1992a, 267:4479-4488.
Gudermann T, Nichols C, Levy F O, et al. Ca~(2+) mobilization by the LH receptor expressed in Xenopus ooeytes independent of 3’, 5’-cyclic adenosine monophosphate formation: evidence for parallel activation of two signaling pathway [J]. Mol Endocrinol, 1992b, 6:272-278.
Hagiwara H, Shibasaki S, Ohwada N. Ciliogenesis in the human oviduct epithelium during the normal menstrual cycle [J]. J Electron Microsc, Tokyo, 1992, 41(5):321.
Haisenleder D J, Ortolono G A, et al. Differential regulation of gonadotropin subunit gene expression by gonadotropin-releasing hormone pulse amplitude in female rats[J]. Endocrinology, 1990, 127:1869-1875.
Heckert L L, Griswold M D. Expression of follicle-stimulating hormone receptor mRNA in rat testes and Sertoli cells [J]. Mol Endocrinol, 1991, 6:70-80.
Heritier A G, Stettler O, Dubois A M. Induction of pituitary cell type differentiation by delta sleep inducing peptide (DSIP)[J]. Neuroendocrinology, 1994, 59(5):477.
Herrero M B, Viggiano J M, Boquet M,et al. Prostaglandins modulation of mouse and human sperm capacitation[J]. Prostaglandins Leukot Essent Fatty Acids, 1997, 57(3):279-284.
Herrlich A, Kuhn B, Grosse R, et al. Involvement of Gs and Gi proteins in dual coupling of the luteinizing hormone receptor to adenylyl cyelase and phospholipase C[J]. Biol Chem, 1996, 271:16764-16772.
Hollis D E, Frith P A, Vaughan J D, et al. Ultrastructural changes in the oviductal epithelium of merino ewes during the oestrus cycle[J]. The American Journal of Anatomy, 1984, 171:441-456.
Jayes F C, Britt J H, Esbenshade K L. Role of gonadotropin-releasing hormone pulse frequency in differential regulation of gonadotropins in the gilt [J]. Biology of Reproduction, 1997, 56:1012-1019.
Johann S, Hyesook Y, MyoungKun J, et al. Orientation of Follicle-stimulating Hormone (FSH) Subunits Complexed with the FSH Receptorβsubunit toward the N terminus of exodomain andαsubunit to exoloop[J]. Biol Chem, 2003, 278(48):47868-47876.
Kangasniemi M, Kaipia A, Toppari J, et al. Cellular regulation of follicle-stimulating hormone (FSH) binging in rat seminiferous tubules [J]. Androl, 1990, 11(4):336-43.
Keith E Latham, F Dale, M Bautista, et al. Comparison of protein synthesis patterns in mouse cumulus cell and granulose cells: Effects of follicle-stimulating hormone and insulin on granulose cell differentiation in vitro[J]. Biology of Reproduction, 1999, 61:482-492.
Kinne-Saffran E, Kinne P K H. Membrane isolation strategy techniques markers [J]. Methods Enzymol, 1989, 172: 3-17.
Kliesch S, Penttila T L, Gromoll J, et al. Stage specific expression of the FSH receptor during rat spermatogenesis [J]. Mol Cell Endocrinol, 1992, 84:45-49.
Kobayashi M, Yamoto M, Minami S, et al. Effects of estrogen, pituitary gonadotropins and prolactin on immunohistochemical localization of inhibin subunits in the ovary of hypophysectomized female rats[J]. Horm Res, 1992, 37(3):107-112.
Korneyi J L, Lix, Lei Z M, et al. Restoration of human chorionic gonadotropin response in human myornetrial smooth muscle cells by treatment with follicle-stimulating hormone (FSH): evidence for the presence of FSH receptors in human myometrium [J]. Eur J Endocrinol, 1996, 134(3):225.
Kreiner D, Liu H C, Itskovitz J, et al. Follicular fluid estradiol and progesterone are markers of preovulatory oocyte quality [J]. Fertil Steril, 1987, 48:991-994.
Kuhn B, Gudermann T. The luteinizing hormone receptor activates phospholipase C via preferential coupling to Gi2 [J]. Biochemistry, 1999, 38:12490-12498.
Kumar T R, Fairchild-Huntress V, Low M J. Gonado-trope-specific expression of the human follicle-stimulating hormone-subunit gene in pituitaries of transgenic mice[J]. Mol Endocrinol, 1992, 6: 81-90.
Leese H J. The formation and function of oviduct fluid[J]. Reprod Fertil, 1988, 82: 843-856.
Lei Z M, Mishra S, Zou W, et al. Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene [J]. Mol Endocrinol, 2001, 15(1):184-200.
Lei ZM, Rao CV, Kornyei JL, et al. Novel expression of human chorionic gonadotropin/luteinizing hormone receptor gene in brain [J].Endocrinology, 1993, 132(5):2262-2270.
Leo E, Reichert JR. The Follice-stimulating hormone (FSH) receptor in testis: interaction of signal transduction and properties of the purified receptor [J]. 1989, 40:13-26.
Libersky E A, Boatman D E. Effects of progesterone on in vitro sperm capacitation and egg penetration in the golden hamster[J]. Biol Reprod, 1995, 53(3):483-487.
Lindsay A M, Channing CP. Comparison of the stimulatory sffects of ovine, porcine and human follicle-stimulating hormone and of ovine and human luteinizing hormone on theaccumulation of cyclic AMP by porcine granulosa cells [J]. Journal of Endocrinology, 1979, 80: 9-20.
Madej A, StuPuieki P. Peripheral plasma levels of LH in the cow through the estrus cycle [J]. Endocrinology, 1975, 65(2):121-125(Polnad).
Mario A, Francesca F, Debgrah L, et al.The Lutropin/Choriogonadotropin Receptor, A 2002 Perspective[J]. Endocrine Reviews, 2002, 23(2):141-174.
Maylayer J R, Hansen P J, Buhi W C. Secretion of proteins by cultured bovine oviductals collected from estrus through early diestrus [J].Exp Zool, 1988, 248(3):345-353.
McFarland K C, Sprengel R, Phillips H S, et al. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family [J]. Science, 1989, 245(4917):494-499.
Means A R. Peptide hormone“receptors”: specific binding of 3H-FSH to Testis [J]. Endocrinology, 1972, 90(1):39.
Medeiros C O, Parrish J J. Changes in Triticum vulvaris (WGA) binding to bovine sperm during capacitation [J]. Anim Sci, 1991, 69(1):460-461.
Meduri G, Charnaux N, Driancourt M A, et al. Follicle-stimulating hormone receptors in oocytes?[J]. J Clin Endocrinol Metab, 2002, 87:2266-2276.
Menchaca A, Rubianes E. Relation between progesterone concentrations during the early luteal phase and follicular dynamics in goats [J]. Theriogenology, 2002, 57(1):1411-1419.
Michael D, Griswold, Leslie Heckert, et al. The molecular biology of the FSH Receptor[J].Steroid Biochem Molec Biol,1995,53:215-218.
Mizrachi D, Shemesh M. Expression of functional luteinising hormone receptor and its messenger ribonucleic acid in bovine cervix: luteinising hormone augmentation of intracellular cAMP, phosphate inositol and cyclooxygenase [J]. Molecular and Cellular Endocrinology, 1999,157 (1-2):191-200.
Murray MK. Epithelial lining of the sheep ampulla oviduct undergoes pregnancy associated morphological changes in secretory status and cell height [J]. Biol Reprod, 1995, 53(3): 653-663.
Muyan M, Ryzmikiewicz D M, Boime I. Secretion of litropin and follitropin from transfected GH3 cells: evidence for separate secretory pathways [J]. Mol Endocrinol, 1994, 8:1789-1797.
Nathalie Lalevee, Christian Rogier, Frederic Becq, et al. Acute effects of adenosine triphosphates, cyclic3’, 5’-adenosing monophosphates and follicle-stimulating hormone on cytosolic calcium level in cultured immature rat sertoli cells [J]. Biology of Reproduction, 1999, 61:343-352.
Nakamura F, Suzaki Y, Yoshimura F. Immunohistochemical and ultrastructural study of anterior pituitary cells in the female Afghan pika, Ocbotone rufescens [J]. Cell Tissue Res, 1986, 244(3):627.
Naz RK, Kaplan P. Interleukin-6 enhances the fertilizing capacity of human sperm by increasing capacitation and acrosome reaction [J]. Androl, 1994, 15(3):228-233.
Novak S, Treacy B K, Almeida F R, et al. Regulation of IGF-I and porcine oviductal secretory protein (pOSP) secretion into the pig oviduct in the peri-ovulatory period, and effects of previous nutrition[J]. Reprod Nutr Dev, 2002, 42(4):355.
Odor D L, Gaddum R P, Rumery RE, et al. Cyclic variations in the ovidutal ciliated cells during the menstrual cycle and after estrogen treatment in the pig-tailed monkey, Macaca nemestrina [J]. Anatomical Record, 1980, 198:35-57.
O Shaughnessy P J, Dudjey K, Rajapaksha W R. Expression of follicle stimulating hormone receptor mRNA during gradual development[J]. Mol Cell Endocrinol, 1996, 125(122):169-175.
Padmanab-han V, McFadden K, Mauger D T, et al. Neuroendocrine control of Follicle-Stimulating Hormone (FSH) Secretion .I. Direct evidence for separate episodic and basal components of FSH secretion [J]. Endocrinology, 1997, 138:424-432.
Parmentier M, Libert F, Maenhaut C, et al. Molecular cloning of the thyrotropin receptor[J]. Science, 1989, 246(4937):1620-1622.
Patsoula E, Loutradis D, Drakakis P, et al. Expression of mRNA for the LH and FSH receptors in mouse oocytes and preimplantation embryos[J]. Reproduction, 2001, 3:455-461.
Pierce J G, Parsons T F. Glycoprotein hormones: structure and function [J]. Annu Rev Biochem, 1981, 50:465-495.
P J Pernicone, B W scheithauer, E Horrath et al. Pituitary and Sellar Region Histology for Pathologists, Second edition, Edited by Stephen S stembery[J].Lippincott Raren Publishers. Philololelaphia, 1997, 1053-1074.
Pubois P M, Amraoui A E, Heritier A G. Development and Differentiation of pituitary cells[J]. Microscopy Rec and techeique, 1997, 39 (2):98.
Rajagopalan-Gupta R, Lamm MLG, Mukherjee S, et al. Luteinizing hormone/choriogonadot-ropin receptor-mediated activation of heterotrimeric guanine nucleotide binding proteins in ovarian follicular membranes[J]. Endocrinology, 1998, 139:4547-4555.
Rajagopalan-Gupta R, Rasenick M, Hunzicker-DunnM. Luteinizing hormone/choriogonadotropin-dependent, cholera toxincatalyzed adenosines5’-diphosphate (ADP)-ribosylation of the long and short forms of Gsαand pertussis toxin-catalyzed ADP-ribosylation of Giα[J]. Mol Endocrinol, 1997, 11:538-549.
Ranniki A S, Zhang F P, Huhtaniemi I T. Ontogeny of follicle stimulating hormone receptor gene expression in the rat testis and ovary[J]. Mol Cell Endocrinol, 1995, 107:199-208.
Rao C V, Lei Z M. Consequences of targeted inactivation of LH receptors[J]. Mol Cell Endocrinol, 2002, 187(1-2):57-67.
Sarkar D K, Fink G. Luteinizing hormone releasing hormone in pituitary stalk plasma from long-term ovsriectomized rats: effects of steroids [J]. J Endocrin, 1980, 86:511-514.
Schaefer M, Hofmann T, Schultz G, et al. A new prostaglandin E receptor mediates calcium influx and acrosome reaction in human spermatozoa [J]. Proc Natl Acad Sci USA,1998,95(6):3008-3013.
Segi E, Haraguchi K, Sugimoto Y, et al. Expression of messenger RNA for prostaglandin E receptor subtypes EP4/EP2 and cyclooxygenase isozymes in mouse periovulatory follicles and oviducts during superovulation[J]. Biol Reprod, 2003, 68(3): 804-811.
Serge Lapointe, Robert Sullivan, Marc-Andte Sirard. Binding of a bovine oviductal fluid catalase to mammalian spermatozoa[J]. Biol Reprod, 1998, 58:747-753.
Shemesh M, Hansel W. Levels of prostaglandin F (PGF) in bovine endometrium, uterine venous, ovarian arterial and jugular plasma during the estrous cycle [J].Proc Soc Exp Biol Med, 1975, 148:123-126.
Shemesh M, Mizrachi D, Gurevich M, et al. Follicle-stimulating hormone receptor and its messenger ribonucleic acid are present in the bovine cervix and can regulate cervical prostanoid synthesis [J]. Biol Reprod, 1999, 61(3):776-784.
Sibley P E, Harper M E, Joyce BG, et al. The immunocytochemical detection of protein hormones in human prostatic tissues[J]. Prostate, 1981, 2(2): 175-185.
Sirimanne J W W, Jones M A. Stage-specific alterations of membrane glycoproteins from the rabbit uterine tract epithelium [J]. Sciences, 1998, 91:135-144.
Smith M S, M.E Freeman, J D Neill. The control of Progesterone secretion during the estrous cycleand early pseudopregnancy in the rat: prolactin, gonadotrophin and steroid levels associated with rescue of the corpus luteum of pseudonregnancy [J]. Endocrinology, 1975, 96:219-226.
Smith T T, Yanagimachi R. Attachment and release of spermatozoa from caudal isthmus of the hamster oviduct [J]. J Reprod Fertil, 1991, 91:567-573.
Smith T T, Yanagimachi R. The viability of hamster spermatozoa stored in the isthmus of the oviduct: the importance of the sperm-epithelium contact for sperm survival [J]. Biol Reprod, 1990, 42(3):450-457.
Sonoda N, Katabuchi H, Tashiro H, et al. Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages[J]. Placenta, 2005, 26(4): 298-307.
Spilman C H. In: Proceedings of a meeting of the task force on methods for regulation of ovum transport (Harper M J K et al eds), 1975, 197-211.
Sprengel R. The testicular receptor for follicle-stimulating hormone structure and functional expression of cloned cDNA[J]. Mol Endocrinol, 1990, 4:525-530.
Sueldo C E, Berger T, Kletzky O & Marrs RP. Serninal prolactin concentration and reproductive capacity[J]. Fertil Steril, 1985, 43(4):632-635.
T Minegishi, S Igarashi, K Nakamura. Functional expfession of the recombinant human FSH receptor[J]. Endocrinol, 1994, 141:369-375.
Timonthy Smith. The mdulation of sperm function by the oviductal epithelium [J]. Biol Reprod, 1998, 58:1102-1104.
Tsai-Morris C H, Buczko E, Wang W, Dufau ML. Intronic nature of therat luteinizing hormone receptor gene defines a soluble receptor subspecies with hormone binding activity [J]. Biol Chem, 1990, 265(11): 19385-19388.
Vannier B, Loosefelt H, Medum G, et al. Anti-human FSH receptor monoclonal antibodies: immunochemical and immunocytochemical characterization of the receptor [J]. Biochemistry, 1996, 35(5):1358-1366.
Verdugo P, Villalon M. Functional anatomy of the fallopian tube. In infertility: male and female. ed by Insler V, Lunenfeld B. 2nd.Churchill Livingstone. Edinburgh. London. Madrid. Melbourne.New York. Tokyo, 1993:11.
Verhage H G, Fazleabas A T, Donnelly K M. The in vitro synthesis and release of proteins by the human oviduct[J]. Endocrinology, 1988, 122(4):639.
Verhage H G, Fazleabas A T. The in vitro synthesis of estrogen2-dependent proteins by the baboon (Pap ioanubis) oviduct[J]. Endocrinology, 1988, 123(1):552.
Verhage H G, Mavrogianis P A, Boomsma R A, et al. Immunologic and molecular characterization of an estrogen-dependent glycoprotein in the rhesus (M acacamulat ta) oviduct[J]. Biol Reprod, 1997, 57(3):525.
Verhage H G, Mavrogianis P A, O’Day-Bowman M B, et al. Characteristics of an oviductal glycoprotein and its potential role in the fertilization process[J]. Biol Reprod, 1998, 58(5):1098-1101.
Vizcarra J A, Wettemann R P, et al. effect of gonadotropin-releasing hormone (GnRH) pulse frequency on serum and pituitary concentrations of luteinizing hormone and follicle-stimulating hormone, GnRH receptors, and messenger ribonucleic acid for gonadotropin subunits in cows [J]. Endocrinology, 1997, 138:594-601.
Wandji S A, Pelletier G, Salesse R, et al. Ontogeny of cellular localization of 125I-labeled insulin-like growth factor-I, 125I-labeled follicle-stimulating hormone, and 125I-labeled human chorionic gonadotropin binding sites in ovaries from bovine fetuses and neonatal calves [J]. Biol Reprod, 1992, 47:814-822.
Wegner C C,Killian G J. Origin of oestrus-associated glycoproteins in bovine oviductal fluid[J]. Reprod Fertil, 1992, 95(3):841-845.
W Zheng, M S Magid, E E Kramer, et al. Chen Follicle-stimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube [J]. Am J Pathol, 1996 January, 148(1): 47-53.
Xie Y B, Wang H, Segaloff D L. Extracellular domain of lutropin/chorogonadotropin receptor expressed in transfected cells binds choriogonadotropin with high affinity [J]. Biol Chem, 1990, 265(12): 21411-21414.
Xing W, Danilovich N, Sairam M R. Orphan receptor chicken ovalbumin upstream promoter transcription factors inhibit steroid factor-1,upstream stimulatory factor, and activator protein-1 activation of ovine follicle-stimulating hormone receptor expression via composite cis-elements [J]. Biology of Reproduction, 2002, 66(6):1656-1666.
Xu Z, Garverick H A, Smith G W, et al. Expression of follicle-stimulating hormone and luteinizing hormone receptor messenger ribonucleic acids in bovine follicles during the first follicular wave [J]. Biol Reprod 1995, 53:951-957.
Yen S C, sffe R B, arbieri R L Reproductive endocrinology[J]. AmWB Saunders Company 1999, 4:101-102.
Yuan W, Lucy M C, Smith M F. Messenger ribonucleic acid for insulin-like growth factors I andⅡ, insulin-like growth factor-binding protein-2, gonadotropin receptors, and steroidogenic enzymes inporcine follicles[J]. Biology of Reproduction, 1996, 55(11): 1045-1054.
Zachos N C, Billiar R B, Albrecht E D, et al. Developmental regulation of Follicle-stimulating hormone receptor messenger RNA expression in the baboon fetal ovary[J]. Biol Reprod, 2003, 68:1911-1919.
Zhang F P, Rannikko A, Huhtaniemi I. Isolation and characterization of testis-specific cDNAs for luteinizing hormone beta-subunit in the rat[J]. Biochem Biophys Res Commun, 1995, 210(3): 858-865.
Zheng M, Shi H, Segaloff D L, Van Voorhis B J. Expression and localization of luteinizing hormone receptor in the female mouse reproductive tract[J]. Biology of Reproduction, 2001, 64:179-187.
Ziecik A J, Bodek G, Biltek A, Kaczmarek M, Waclawik A. NongonadalLH receptors, their involvement in female reproductive function and a new applicable approach [J]. Vet J, 2005, 169(1):75-84.
Ziecik AJ,Tilton JE,Espana F,et al. Effect of human chorionic gonadotropin on preovulatory luteinizing hormone surge and ovarian hormone secretion in pigs[J].J Anim Sci,1987,64:1134-1143.