MicroRNA表达与非小细胞肺癌放疗敏感性关系的研究
摘要
目的研究放疗敏感和不敏感非小细胞肺癌病人microRNA表达谱的差异,并且探索这些差异的潜在应用价值。材料和方法根据治疗中肿瘤的局部控制率、缓减率以及复发率和生存期,将30位实施过术后放射治疗的非小细胞肺癌病人分成敏感组和不敏感组。用microRNA表达谱芯片检测两组病人microRNA的表达差异,用实时定量RT-PCR对芯片结果进行验证。结果与不敏感组相比,辐射敏感组中发现有5个miRNA (miR-126, miR-let-7a, miR-495, miR-451, miR-128b)表达显著上调,其中miRNA-126的变化最为显著；7个miRNA(miRNA-130a, miRNA-106b, miRNA-19b, miRNA-22, miRNA-15b, miRNA-17-5p, miRNA-21)表达显著下调。Real-time PCR结果与芯片结果一致。结论本研究发现的12种microRNA可能与非小细胞肺癌的放射敏感性有一定的相关性。microRNA-126是表达差异最显著的microRNA,提示其可能在非小细胞肺癌的辐射反应中发挥重要作用
Objective To investigate the different miRNA expression profile of radiotherapy sensitive and resistant patients of non-small cell lung cancer. Methods Thirty non-small cell lung cancer patients were divided into radiotherapy sensitive group and resistant group. Expression profile of miRNA in these two groups was detected by a microarray assay and the results were validated by quantitative RT-PCR. Results Comparing with resistant patients, five miRNA (miR-126, miR-let-7a, miR-495, miR-451,miR-128b) were significantly up-regulated and seven miRNA (miRNA-130a, miRNA-106b,miRNA-19b,miRNA-22,miRNA-15b, miRNA-17-5p, miRNA-21)were greatly down-regulated in radiotherapy sensitive group. Conclusion These twelve miRNA found in the present study may be correlated with the radiotherapy sensitivity of non-small cell lung cancer.
Objective To investigate the different miRNA expression profile of radiotherapy sensitive and resistant patients of non-small cell lung cancer. Methods Thirty non-small cell lung cancer patients were divided into radiotherapy sensitive group and resistant group. Expression profile of miRNA in these two groups was detected by a microarray assay and the results were validated by quantitative RT-PCR. Results Comparing with resistant patients, five miRNA (miR-126, miR-let-7a, miR-495, miR-451,miR-128b) were significantly up-regulated and seven miRNA (miRNA-130a, miRNA-106b,miRNA-19b,miRNA-22,miRNA-15b, miRNA-17-5p, miRNA-21)were greatly down-regulated in radiotherapy sensitive group. Conclusion These twelve miRNA found in the present study may be correlated with the radiotherapy sensitivity of non-small cell lung cancer.
引文
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