过瘤胃产品包被技术的研究
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摘要
采用不同的材料和配比制得过瘤胃蛋氨酸并对蛋氨酸的包被和利用效果进行评价。
     本试验采用湿法制粒制得蛋氨酸球形颗粒,喷雾干燥法包衣制得过瘤胃蛋氨酸微胶囊产品。壁材选用丙烯酸树脂IV号、羟丙甲纤维、乙基纤维素、丙烯酸树脂IV号与羟丙甲纤维素的混合材料以及丙烯酸树脂IV号与乙基纤维素的混合材料。采用不同的配比,以及不同的壁材用量,制作出首批21种过瘤胃蛋氨酸产品;混合壁材处理组通过均匀试验设计处理,得出最佳配比结果。再用此最佳配比的壁材,不同的用量,制作出5种过瘤胃蛋氨酸产品。最终通过体外法检验其稳定性,选出效果最好的一种壁材,丙烯酸树脂IV号与乙基纤维素1:2的混合材料包被的过瘤胃蛋氨酸产品。
     过瘤胃蛋氨酸在饲喂不同日粮的奶山羊瘤胃中的瘤胃稳定性的检验,试验选择3只健康的奶山羊,安装永久性瘤胃瘘管,尼龙袋法测定过瘤胃蛋氨酸的瘤胃动态降解率。设计精粗比为I(50:50)、II(30:70)、III(70:30)三种日粮,3*3拉丁方设计。结果显示,在饲喂三种日粮(I、II、III组)的奶山羊瘤胃中的PH值范围分别为6.08-6.40、6.43-6.90、5.50-5.90,对应的过瘤胃蛋氨酸有效通过率分别为:有效降通过率在分别为72.30%、70.29%、68.85%(奶山羊瘤胃食糜流通速率Kp=0.02/h), 78.69%、76.76%、73.30% (高产奶牛瘤胃食糜流通速率Kp=0.06/h),包被效果较好,没有出现包被不严的现象。
     添加不同量的过瘤胃蛋氨酸检测过瘤胃蛋氨酸在瘤胃后消化道的释放特性的检验以及体内氮沉积的影响。选用三只健康的奶山羊,三个处理组(I、II、III),添加过瘤胃蛋氨酸的量分别为0g、15g、30g,3*3拉丁方设计。结果显示添加过瘤胃蛋氨酸的日粮,粪样中氮的含量没有明显的变化(P>0.05),尿液中氮的含量显著提高(III>II>I)(P<0.005),过瘤胃蛋氨酸在瘤胃后的释放率II组和III组分别达到93.33%和95.00%,说明奶山羊全消化道对此过瘤胃蛋氨酸的吸收良好,制得的过瘤胃蛋氨酸在瘤胃后消化道释放良好,没有出现包被过度的情况。食入氮的转化为体组织沉积效率显著提高(II>III>I)(P<0.05)。
Experiments were conducted to study the producing techniques and the effectiveness of protection and application of Rume n bypass methionine(RPMet).
     The experiment using wet granul ation be spherical methionine ,Coated by spray drying microencapsulation of ruminally protected methionine product. Wa ll ma terial used in this experiment IV, acrylic resins, hydroxypropyl A fiber, ethyl cellulose, acrylic resin IV, and HPMC in the mixed material and acrylic resin and ethyl cellulose IV, the hybrid materials. Different ratio, and different amount of wall material, to produce the first batch of 21 ruminally protected methionine product; Mixed wall ma terial treated by uniform design treatment, the best ratio of results. Then the best ratio of wall material, different dosage, to produce five kinds of rumi nally protected methionine product. Final test its stability by in vitro me thod to select the best of a wall material.
     Fed diff erent diets in the rumen of dairy goats RPMet effective degradation rate differences ,Select three healthy dairy goats, install a permanent rumen fistula ,Dynami c determination RPMet rumen degradation rate in situ. Design of forage to concentrate ratio was I (50:50), II (30:70), III (70:30) of three diets, 3 * 3 Latin square experiment design. The results showed that feeding of three diets (I, II, III group) of dairy goat rumen, PH value in the range of PH6.08-6.40,PH6.43-6.90,PH5.50-5.90. Methionine corresponding to the effective rumen degradation rates were: 72.30%、 70.29%、68.85% (Kp=0.02/h ),78.69%、76.76%、73.30% (Kp=0.06/h ).
     Adding diff erent amounts of ruminally protected methionine testing all dairy goat rumen digestive tract on the absorption of methionine and the in vivo effects of nitrogen deposition. Selected three healthy dairy goats, three treatment groups (I, II, III), Add the amount of me thionine in rumen were 0g, 15g, 30g, 3 * 3 Latin square experiment design. The results revealed that the addition of rumi nally protected methionine in the diet, Fecal nitrogen content did not change significantly (P> 0.05), the nitrogen content in urine was significantly increased (III> II> I) (P <0.005), This shows the total tract of dairy goat rume n absorption of methionine good. Ingested nitrogen into tissue significantly increased the deposition efficiency (II> III> I) (P <0.05).
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