辽宁、吉林和朝鲜人参对小鼠细胞免疫影响的比较研究
摘要
目的:
科学地比较辽宁、吉林和朝鲜三地人参对小鼠淋巴细胞增值活性、血清IL-2含量和NK细胞活性等细胞免疫药效作用的强弱,以明确人参产地与其细胞免疫药效的关系,为人参的临床应用,促进辽宁经济的发展提供可靠的数据。
方法:
选用健康雌性昆明小鼠80只,随机分成8组,各组分笼常规饲养,其中A1组:正常对照组,A2组:辽宁人参组,A3组:吉林人参组,A4组:朝鲜人参组;B1组:免疫抑制对照组,B2组:免疫抑制辽宁人参组,B3组:免疫抑制吉林人参组,B4组:免疫抑制朝鲜人参组。对B1～4组小鼠采用国际公认的免疫抑制造模方法建立免疫抑制模型,即将小鼠腹腔注射环磷酰胺注射液(80mg/kg),隔日1次,总计给药4次。免疫抑制组造模成功后A2、A3、A4、B2、B3、B4组小鼠按对应产地的人参水煎剂灌胃,A1和B1组以生理盐水灌胃。各组灌胃剂量为0.4ml/只,每日1次,连续12天,并于12天后处死各组小鼠,检测小鼠淋巴细胞增值活性、血清IL-2含量和NK细胞活性等指标。
结果:
1.三地人参均能提高正常小鼠和免疫抑制小鼠脾指数(p<0.05),并均能将免疫抑制小鼠脾指数提高到正常水平(p>0.05),三地人参对正常小鼠和免疫抑制小鼠脾指数的影响的比较无统计学意义(p>0.05)。
2.三地人参均能提高正常小鼠和免疫抑制小鼠淋巴细胞增值活性(p<0.05),三地人参对正常小鼠和免疫抑制小鼠淋巴细胞增值活性影响的比较无统计学意义(p>0.05),但辽宁人参能将免疫抑制小鼠淋巴细胞增殖活性提高到正常水平(p>0.05)。
3.三地人参均能促进正常小鼠和免疫抑制小鼠血清IL-2含量提高(p<0.05),并均能将免疫抑制小鼠血清IL-2含量提高到正常水平(p>0.05),三地人参相比,辽宁人参对提高正常小鼠和免疫抑制小鼠血清IL-2含量的作用更显著(p<0.05)。
4.三地人参均能提高正常小鼠和免疫抑制小鼠NK细胞的活性(p<0.05),并均能将免疫抑制小鼠NK细胞活性提高到正常水平(p>0.05),三地人参对正常小鼠和免疫抑制小鼠NK细胞活性的影响的比较无统计学意义(p>0.05)。
结论:
1.三地人参可提高正常及免疫抑制小鼠脾指数和NK细胞活性,并能将免疫抑制小鼠脾指数和NK细胞活性提高到正常水平,三地人参间无差异。
2.三地人参可提高正常及免疫抑制小鼠淋巴细胞增殖活性,辽宁人参能将免疫抑制小鼠淋巴细胞增殖活性提高到正常水平,三地人参间无差异。
3.三地人参可提高正常及免疫抑制小鼠血清IL-2含量,并能将免疫抑制小鼠血清IL-2含量提高到正常水平,辽宁人参对正常小鼠和免疫抑制小鼠血清IL-2含量的作用要强于吉林和朝鲜人参。
Purpose:
To scientifically compare the effect of ginseng from different regions on Cellulate Immunity Medicine Effect, like activity of lymphocyte transformation, content of IL-2 in Seru and Activity of Natural Killer Cell, as to identify the relationship between the origin of ginseng and cellulate immunity medicine effect, which can provide reliable statistics to the development of Liaoning economy and to establish the Brand of“Liaoning Ginseng”.
Material and method:
Select 80 healthy female rats, which are divided into eight groups. Each group is caged by normal feeding, among which A1: Normal Comparison Group; A2: Liaoning Ginseng Group; A3: Jilin Ginseng Group; A4: Korean Ginseng Group; B1: Inhibited Immunity Comparison Group; B2: Inhibited Immunity Liaoning Ginseng Group; B3: Inhibited Immunity Liaoning Ginseng Group; B4: Inhibited Immunity Korean Ginseng Group. For the rats in Group B, the Inhibited Immunity Model is made by the model-making method which is recognized internationally, i.e. to inject cyclophosphamide to the rats’belly 80 mg/kg, once the other day, four times in total. When model-making is successful for Inhibited Immunity Group, lavage rats stomach who are in A2, A3, A4, B2, B3, B4 using ginseng water; Lavage rats stomach who are in A1 and B1 using Brine, 0.4 ml/one rat, once per day, continuously, twelve days later, examine the index of Activity of lymphocyte transformation, IL-2 content in Seru and Activity of Natural Killer Cell.
Results:
1 All the ginseng from the three regions can increase the spleen index of normal rats and Inherit Immunity Rats(p<0.05); and increase the spleen index of Inherit Immunity Rats to a normal level(p>0.05).There is no comparing statistics meaning of the impact on spleen index for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05).
2 All the ginseng from the three regions can accelerate the Lymphocyte Transformation Activity for both Normal rats and Inherit Immunity rats(p<0.05),There is no comparing statistics meaning of the impact on Lymphocyte Transformation Activity for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05); Yet Liaoning Ginseng can increase the Lymphocyte Transformation Activity of Inherit Immunity Rats to a normal level(p>0.05).
3 All the ginseng from the three regions can accelerate the content increase of IL-2 in Seru for both Normal rats and Inherit Immunity rats(p<0.05); and increase the IL-2 content in seru of Inherit Immunity Rats to a normal level (p>0.05),. Among the ginseng from the three regions, Liaoning ginseng can better increase the IL-2 content in Seru for both Normal Rats and Inherit Immunity Rats (p<0.05).
4 All the ginseng from the three regions can accelerate the increase of NK activity for both Normal rats and Inherit Immunity Rats (p<0.05) , and increase the NK cell Activity of Inherit Immunity Rats to a normal level (p>0.05),.There is no comparing statistics meaning of the impact on NK Activity for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05).
Conclusion:
1 All the ginseng from the three regions can accelerate the spleen index and the NK cell activity for both Normal rats and Inherit Immunity rats, and can increase them to a normal level.
2 All the ginseng from the three regions can accelerate the Lymphocyte Transformation Activity for both Normal rats and Inherit Immunity rats, Liaoning ginseng can increase the Lymphocyte Transformation Activity of Inherit Immunity rats to a normal level, with no difference among the ginseng from the three region.
3. All the ginseng from the three regions can increase the content of IL-2 in Seru for both Normal rats and Inherit Immunity rats, and can increase the IL-2 in Seru of inherit immunity rats to a normal level. The impact of Liaoning ginseng on IL-2 in Seru of normal rats and inherit immunity rats are stronger than that of Jilin and Korea.
科学地比较辽宁、吉林和朝鲜三地人参对小鼠淋巴细胞增值活性、血清IL-2含量和NK细胞活性等细胞免疫药效作用的强弱,以明确人参产地与其细胞免疫药效的关系,为人参的临床应用,促进辽宁经济的发展提供可靠的数据。
方法:
选用健康雌性昆明小鼠80只,随机分成8组,各组分笼常规饲养,其中A1组:正常对照组,A2组:辽宁人参组,A3组:吉林人参组,A4组:朝鲜人参组;B1组:免疫抑制对照组,B2组:免疫抑制辽宁人参组,B3组:免疫抑制吉林人参组,B4组:免疫抑制朝鲜人参组。对B1～4组小鼠采用国际公认的免疫抑制造模方法建立免疫抑制模型,即将小鼠腹腔注射环磷酰胺注射液(80mg/kg),隔日1次,总计给药4次。免疫抑制组造模成功后A2、A3、A4、B2、B3、B4组小鼠按对应产地的人参水煎剂灌胃,A1和B1组以生理盐水灌胃。各组灌胃剂量为0.4ml/只,每日1次,连续12天,并于12天后处死各组小鼠,检测小鼠淋巴细胞增值活性、血清IL-2含量和NK细胞活性等指标。
结果:
1.三地人参均能提高正常小鼠和免疫抑制小鼠脾指数(p<0.05),并均能将免疫抑制小鼠脾指数提高到正常水平(p>0.05),三地人参对正常小鼠和免疫抑制小鼠脾指数的影响的比较无统计学意义(p>0.05)。
2.三地人参均能提高正常小鼠和免疫抑制小鼠淋巴细胞增值活性(p<0.05),三地人参对正常小鼠和免疫抑制小鼠淋巴细胞增值活性影响的比较无统计学意义(p>0.05),但辽宁人参能将免疫抑制小鼠淋巴细胞增殖活性提高到正常水平(p>0.05)。
3.三地人参均能促进正常小鼠和免疫抑制小鼠血清IL-2含量提高(p<0.05),并均能将免疫抑制小鼠血清IL-2含量提高到正常水平(p>0.05),三地人参相比,辽宁人参对提高正常小鼠和免疫抑制小鼠血清IL-2含量的作用更显著(p<0.05)。
4.三地人参均能提高正常小鼠和免疫抑制小鼠NK细胞的活性(p<0.05),并均能将免疫抑制小鼠NK细胞活性提高到正常水平(p>0.05),三地人参对正常小鼠和免疫抑制小鼠NK细胞活性的影响的比较无统计学意义(p>0.05)。
结论:
1.三地人参可提高正常及免疫抑制小鼠脾指数和NK细胞活性,并能将免疫抑制小鼠脾指数和NK细胞活性提高到正常水平,三地人参间无差异。
2.三地人参可提高正常及免疫抑制小鼠淋巴细胞增殖活性,辽宁人参能将免疫抑制小鼠淋巴细胞增殖活性提高到正常水平,三地人参间无差异。
3.三地人参可提高正常及免疫抑制小鼠血清IL-2含量,并能将免疫抑制小鼠血清IL-2含量提高到正常水平,辽宁人参对正常小鼠和免疫抑制小鼠血清IL-2含量的作用要强于吉林和朝鲜人参。
Purpose:
To scientifically compare the effect of ginseng from different regions on Cellulate Immunity Medicine Effect, like activity of lymphocyte transformation, content of IL-2 in Seru and Activity of Natural Killer Cell, as to identify the relationship between the origin of ginseng and cellulate immunity medicine effect, which can provide reliable statistics to the development of Liaoning economy and to establish the Brand of“Liaoning Ginseng”.
Material and method:
Select 80 healthy female rats, which are divided into eight groups. Each group is caged by normal feeding, among which A1: Normal Comparison Group; A2: Liaoning Ginseng Group; A3: Jilin Ginseng Group; A4: Korean Ginseng Group; B1: Inhibited Immunity Comparison Group; B2: Inhibited Immunity Liaoning Ginseng Group; B3: Inhibited Immunity Liaoning Ginseng Group; B4: Inhibited Immunity Korean Ginseng Group. For the rats in Group B, the Inhibited Immunity Model is made by the model-making method which is recognized internationally, i.e. to inject cyclophosphamide to the rats’belly 80 mg/kg, once the other day, four times in total. When model-making is successful for Inhibited Immunity Group, lavage rats stomach who are in A2, A3, A4, B2, B3, B4 using ginseng water; Lavage rats stomach who are in A1 and B1 using Brine, 0.4 ml/one rat, once per day, continuously, twelve days later, examine the index of Activity of lymphocyte transformation, IL-2 content in Seru and Activity of Natural Killer Cell.
Results:
1 All the ginseng from the three regions can increase the spleen index of normal rats and Inherit Immunity Rats(p<0.05); and increase the spleen index of Inherit Immunity Rats to a normal level(p>0.05).There is no comparing statistics meaning of the impact on spleen index for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05).
2 All the ginseng from the three regions can accelerate the Lymphocyte Transformation Activity for both Normal rats and Inherit Immunity rats(p<0.05),There is no comparing statistics meaning of the impact on Lymphocyte Transformation Activity for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05); Yet Liaoning Ginseng can increase the Lymphocyte Transformation Activity of Inherit Immunity Rats to a normal level(p>0.05).
3 All the ginseng from the three regions can accelerate the content increase of IL-2 in Seru for both Normal rats and Inherit Immunity rats(p<0.05); and increase the IL-2 content in seru of Inherit Immunity Rats to a normal level (p>0.05),. Among the ginseng from the three regions, Liaoning ginseng can better increase the IL-2 content in Seru for both Normal Rats and Inherit Immunity Rats (p<0.05).
4 All the ginseng from the three regions can accelerate the increase of NK activity for both Normal rats and Inherit Immunity Rats (p<0.05) , and increase the NK cell Activity of Inherit Immunity Rats to a normal level (p>0.05),.There is no comparing statistics meaning of the impact on NK Activity for the normal rats and inherit immunity rats by ginseng from different regions(p>0.05).
Conclusion:
1 All the ginseng from the three regions can accelerate the spleen index and the NK cell activity for both Normal rats and Inherit Immunity rats, and can increase them to a normal level.
2 All the ginseng from the three regions can accelerate the Lymphocyte Transformation Activity for both Normal rats and Inherit Immunity rats, Liaoning ginseng can increase the Lymphocyte Transformation Activity of Inherit Immunity rats to a normal level, with no difference among the ginseng from the three region.
3. All the ginseng from the three regions can increase the content of IL-2 in Seru for both Normal rats and Inherit Immunity rats, and can increase the IL-2 in Seru of inherit immunity rats to a normal level. The impact of Liaoning ginseng on IL-2 in Seru of normal rats and inherit immunity rats are stronger than that of Jilin and Korea.
引文
[1]任仁安,陈瑞华.中药鉴定学.上海:上海科学技术出版社,1986:125
[2]朱永明.辽参与高丽参渊源考.人参研究.2001,13:45-46
[3]张语迟,1王淑敏,宋凤瑞等.市售高丽参与中国红参的质量评价.长春中医药大学学报.2008.24(2): 148-149
[4]李艾莲.人参.北京:中国中医药出版社,2001:3
[5]王林军,康建国,巢志茂等.峨眉山引种人参的研究.中国中药杂志. 1999,4(ll):658-659
[6]张连学,常维春,赵立波等.缺锌对人参生长发育的影响及其防治研究.中药材.1991,14(12):3-5
[7]李新兰,朱蔚华.微量元素对人参愈伤组织生长和皂甙含量的影响.中药材.1993,16(6):3-4
[8]刘立侠,唐树延,许守民等.光质对人参叶绿体结构和光合生理性状的影响.植物学.1993,35(8):588-592
[9]重修政和经史证类备用本草卷六.北京:人民卫生出版社, 1957:145-146
[10]明·李时珍.本草纲目.北京:科学出版社,1998:6
[11]明·谢肇淅.人参谱.养生月刊. 2007, 28(1):45-52
[12]清·陆烜.人参谱.养生月刊.2007,28(4):333-340
[13]唐晖,汪保和,贺洪.人参皂甙Rg1促进小鼠力竭游泳后体能恢复期的作用[J].中国运动医学杂志.2002,21(4):375-378
[14]杨国愉,皇甫恩,苗丹民等.人参皂甙对动物学习记忆影响的研究进展[J].中国行为医学科学.2000,9(3):932
[15]姜正林,吴新民,陈云等.人参皂甙的抗鼠脑缺氧损伤作用[J].中华航海医学杂志. 2000,7(1):28- 32.
[16]赵晓民,宗瑞义,谢湘林等.人参茎叶皂甙和胆碱合用对学习记忆的影响[J].营养学报.2002,22(3):266-269.
[17]李君庆,张香阁,张均田.人参皂甙Rg1抗神经细胞凋亡作用机制的研究[J].药学学报.1997,32(6):406-410
[18] Chen X C, Zhu Y G,Wang X Z, et al. Protective effect ofginseno-side Rg1on dopamine-induced apoptosis in PC12 cells〔J〕.Acta Pharmacol Sin . 2001, 22(8):673-678
[19] hen X C, Fang F, Zhu Y G, et al.Protective effect of ginsenoside Rg1 on MPP(+)-induced apoptosis in SYSY5Y cells〔J〕.J neural Transm. 2003, 110(8):835-845
[20] Chen X C, Chen L M , Zhu Y G, et al. Involvement of CDK4, pRB, and E2F1 in ginsenoside Rg1 protect in grat cortical neu - rons from beta - amyloid induced apoptosis[ J] .Acta Pharmacol Sin.2003,24(12):1259-1264
[21] Scott G I, Colligan P B, Ren B H, et al.Ginsenosides Rb1 and Redecrease cardiac contraction in adult ratventricularmy-ocytes: role of nitricoxide [J].Br J P harmacol.2001,134(6):1159-1165
[22]王莎莉,李静,王亚平.人参皂苷诱导骨髓巨噬细胞表达造血生长因子的研究.解剖学报.2003,34(5):500
[23]陈笛,王莎莉,王亚平.人参总皂苷诱导淋巴细胞表达GM-CSF的实验研究.免疫学杂志.2003,19(6):411
[24]朱卫岗,郭实士.人参多糖对小鼠免疫功能的影响.湖南医科大学学报.1991,16(3):107-110
[25]王本祥,崔景朝,刘爱晶.人参多糖对免疫机能的影响.药学学报.1982,17(1): 66-68
[26] Kim K H, Lee Y S, Jung I S, et al. Acidic Polysaccharide from Panax ginseng, ginsan,induces Thl cell and macphage cytokines and generates LAK cells in synergy with rIL-2 [ J ]. Planta Med.1998,64(2):110-115
[27]龙振洲.医学免疫学.北京:人民卫生出版社,1999:45
[28]张勤,叶再元,杜卫东.人参皂甙对腹腔感染大鼠血清白蛋白及白介素-6影响的实验研究[J].浙江中医学院学报.2003,27(4):66-67
[29]刘轩,魏育林,李波.人参皂苷对脂多糖诱导小鼠肺泡巨噬细胞分泌肿瘤坏死因子的作用及机制探讨.中日友好医院学报.2001,15(2):83
[30]王庭富,孟正木.人参皂苷Rg3对免疫功能的影响.中国药科大学学报.1999, 30(2):133
[31]李卫平,陈敏珠.人参皂甙的免疫调节作用.安徽医科大学学报. 1998,33(5):348-350
[32]刘靖华,陈惠孙,胡德耀.人参皂苷对创伤失血性休克免疫功能的调节作用.第三军医大学学报.2000,22(4):324
[33]蒋艳,王毅,邱全瑛.人参皂甙Rg1及其肠内菌代谢产物Rh1对小鼠免疫细胞功能的影响[J].中国病理生理杂志.2001,17(8):815
[34]沈玲,张均田,刘态.人参增强免疫研究新进展.中草药.1996,27(8):499-502
[35]张仲苗,江波,章荣华等.人参皂甙Rg3对小鼠免疫功能的影响.中药药理与临床.2004,20(6):4-6
[36]胡松华,林锋强.人参皂甙Rb1的免疫佐剂作用[J].中国兽医学报.2003,23(5): 480-482
[37] Bancherv J , Steinmen RM. Dendritric cell and the control .immunity. Nature. 1998,392(6673):245
[38]龙振洲.医学免疫学.北京:人民卫生出版社,1999:105
[39]王毅,郝钰,邱全瑛.人参皂苷Rg1及Rh1对树突状细胞刺激T细胞增殖及LPAK抗肿瘤活性的影响.中国免疫学杂志.2003,19(4):248-252
[40]龙振洲.医学免疫学.北京:人民卫生出版社,1999:100
[41]郭志廷,韦旭斌,梁剑平等.人参总皂苷及其衍生物对小鼠淋巴细胞和NK细胞免疫活性的影响.中国兽医学报.2007,27(5):715-717
[42] Yang Guizhen ,Yu Yongli.effects of ginsenoside on the natural killer cell-interferon-interleukin-2 regulatory network and its tumor inhibiting effect.Journal of Traditional Chinese Medicine.1988,8(2):135
[43]张远强.人参对巨噬细胞影响的定量细胞化学研究等.病理生理学报.1985,1(4):22
[44] Xiaoguang C, Hongyan L, Xiaohong L, et al Cancer cheomp reventive and therapeutic acivities of red ginseng[ J ]. J Ethnopharm acol.1998,60(1):71 -78
[45] Kang K S, Kang B C,Lee B J, et al. Preventive effect of ep icatechin and ginsenoside Rb(2) on the inhibition of gap juctional inter, cellular communication by TPA and H2O2 [ J ] Cancer Lett.2000,152(1):97-106
[46] Wargovich M J. Colon cancer chemop revention with ginseng and other botanicals[J]. J Korean Med Sci.2001,16(l): 81-86
[47] Xie X,Eberding A ,Madera C ,Fazli L ,Jia W,Goldenberg L ,et al . Rh2 Synergistically Enhances Paclitaxel or Mitoxantrone in Prostate Cancer Models[J] The Journal of Urology.2006,175 (5):26–31
[48]陈明伟,姚昱,石志红.人参皂甙Rg3联合化疗对30例非小细胞肺癌的近期疗效观察[J].华南国防医学杂志.2005,19(1):4-6
[49] Suda K, Murakami K, Murata J, et a1.An intestinal bacterial metabolite of ginseng protopanaxadiol saponins inhibits tumot–induced neovascularization[J].J. Trad. Med. 2000,17:144-150
[50]张有为,窦德强,陈英杰等.人参皂甙对人体骨肉瘤细胞U2OS增殖的影响[ J ].中草药. 2001,32(3):232-236
[51]葛迎春,刘平,韩信昌.人参皂Rb1, Rg1, Re和Rh1对Hela细胞的影响[ J ].中药理与临床.1997,13(6):18-20
[52] Kim, H E Oh J H, Lee S, K, et al. Ginsenoside Rh2 induces apop totic cell death in rat C6 glioma via a reactive oxygen-and caspase-dependent but Bcl-X(L)-independent pathway[J]. L ife Sci,1999,65(3):33-40
[53]凌昌全,俞超琴,潘瑞萍等.人参皂甙对人T淋巴细胞白血病细胞株凋亡基因的影响[J].中医杂志.2000,41(3):213-215
[54]王占峰,罗毅男,洪新雨等.人参皂甙Rh2抗肿瘤作用机制的研究[J].北华大学学报.2005,6(1):50-52
[55] Mochizuki M, Yung CY, Kaori M ,et al.Inhibition effect of tumor metastasis in mice by saponins, ginsenoside-Rb2,20(R)- and 20(S) ginsenoside - Rg3, of red ginseng [J]. Biol. Pharm. Bull.1996,18(9):1197-1202
[56] Hasegawa H, Uchiyama M.Antimetasatic efficacy of orally administered girtsenoside Rb1 in dependence no intestinal bacterial hydrolyzing potential and significance of treatment with an active hacterial metabolite[J].Planta. Med. 1998,64:696-700
[57]黄宗锈,陈冠敏,林蔚.人参皂甙延缓衰老的实验研究[J].实用预防医学. 2001,8(5):385
[58]龚国清.人参皂甙等清除超氧阴离子自由基的研究.中国药科大学学报.1991,(1):41-43
[59] Chang M S, Choi K J,Rho H M.PD.人参皂甙诱导自由基排除效果.高丽人参学会杂志.1999,23(l):44-49 [60 ] Wee J J,Hco J N,kim M W,Kang D Y.红参的过氧化防御作用.高丽人参学会杂志.1996,20(2):154-155
[61]韩红梅,杨贵贞.人参三醇型皂甙调整骨髓受抑模型鼠的免疫功能.中国免疫学杂志.1991,7(5):312-316
[62]柳忠辉,王树和,杨贵贞.人参皂甙对外源ACTH所致小鼠免疫功能抑制的调整效应.白求恩医科大学学报.1995,21(4):350-352
[63]宋承吉.对《古代人参基源再认识》的质疑.人参研究.1998,(3):17
[64]张语迟,1王淑敏,宋凤瑞等.市售高丽参与中国红参的质量评价.长春中医药大学学报.2008.24(2): 148-149
[65]龙振洲.医学免疫学.北京:人民卫生出版社.1999:51
[66]杜念兴.兽医免疫学[M] .上海:上海科技出版社.1984:272-274
[67]龙振洲.医学免疫学.北京:人民卫生出版社.1999:131
[2]朱永明.辽参与高丽参渊源考.人参研究.2001,13:45-46
[3]张语迟,1王淑敏,宋凤瑞等.市售高丽参与中国红参的质量评价.长春中医药大学学报.2008.24(2): 148-149
[4]李艾莲.人参.北京:中国中医药出版社,2001:3
[5]王林军,康建国,巢志茂等.峨眉山引种人参的研究.中国中药杂志. 1999,4(ll):658-659
[6]张连学,常维春,赵立波等.缺锌对人参生长发育的影响及其防治研究.中药材.1991,14(12):3-5
[7]李新兰,朱蔚华.微量元素对人参愈伤组织生长和皂甙含量的影响.中药材.1993,16(6):3-4
[8]刘立侠,唐树延,许守民等.光质对人参叶绿体结构和光合生理性状的影响.植物学.1993,35(8):588-592
[9]重修政和经史证类备用本草卷六.北京:人民卫生出版社, 1957:145-146
[10]明·李时珍.本草纲目.北京:科学出版社,1998:6
[11]明·谢肇淅.人参谱.养生月刊. 2007, 28(1):45-52
[12]清·陆烜.人参谱.养生月刊.2007,28(4):333-340
[13]唐晖,汪保和,贺洪.人参皂甙Rg1促进小鼠力竭游泳后体能恢复期的作用[J].中国运动医学杂志.2002,21(4):375-378
[14]杨国愉,皇甫恩,苗丹民等.人参皂甙对动物学习记忆影响的研究进展[J].中国行为医学科学.2000,9(3):932
[15]姜正林,吴新民,陈云等.人参皂甙的抗鼠脑缺氧损伤作用[J].中华航海医学杂志. 2000,7(1):28- 32.
[16]赵晓民,宗瑞义,谢湘林等.人参茎叶皂甙和胆碱合用对学习记忆的影响[J].营养学报.2002,22(3):266-269.
[17]李君庆,张香阁,张均田.人参皂甙Rg1抗神经细胞凋亡作用机制的研究[J].药学学报.1997,32(6):406-410
[18] Chen X C, Zhu Y G,Wang X Z, et al. Protective effect ofginseno-side Rg1on dopamine-induced apoptosis in PC12 cells〔J〕.Acta Pharmacol Sin . 2001, 22(8):673-678
[19] hen X C, Fang F, Zhu Y G, et al.Protective effect of ginsenoside Rg1 on MPP(+)-induced apoptosis in SYSY5Y cells〔J〕.J neural Transm. 2003, 110(8):835-845
[20] Chen X C, Chen L M , Zhu Y G, et al. Involvement of CDK4, pRB, and E2F1 in ginsenoside Rg1 protect in grat cortical neu - rons from beta - amyloid induced apoptosis[ J] .Acta Pharmacol Sin.2003,24(12):1259-1264
[21] Scott G I, Colligan P B, Ren B H, et al.Ginsenosides Rb1 and Redecrease cardiac contraction in adult ratventricularmy-ocytes: role of nitricoxide [J].Br J P harmacol.2001,134(6):1159-1165
[22]王莎莉,李静,王亚平.人参皂苷诱导骨髓巨噬细胞表达造血生长因子的研究.解剖学报.2003,34(5):500
[23]陈笛,王莎莉,王亚平.人参总皂苷诱导淋巴细胞表达GM-CSF的实验研究.免疫学杂志.2003,19(6):411
[24]朱卫岗,郭实士.人参多糖对小鼠免疫功能的影响.湖南医科大学学报.1991,16(3):107-110
[25]王本祥,崔景朝,刘爱晶.人参多糖对免疫机能的影响.药学学报.1982,17(1): 66-68
[26] Kim K H, Lee Y S, Jung I S, et al. Acidic Polysaccharide from Panax ginseng, ginsan,induces Thl cell and macphage cytokines and generates LAK cells in synergy with rIL-2 [ J ]. Planta Med.1998,64(2):110-115
[27]龙振洲.医学免疫学.北京:人民卫生出版社,1999:45
[28]张勤,叶再元,杜卫东.人参皂甙对腹腔感染大鼠血清白蛋白及白介素-6影响的实验研究[J].浙江中医学院学报.2003,27(4):66-67
[29]刘轩,魏育林,李波.人参皂苷对脂多糖诱导小鼠肺泡巨噬细胞分泌肿瘤坏死因子的作用及机制探讨.中日友好医院学报.2001,15(2):83
[30]王庭富,孟正木.人参皂苷Rg3对免疫功能的影响.中国药科大学学报.1999, 30(2):133
[31]李卫平,陈敏珠.人参皂甙的免疫调节作用.安徽医科大学学报. 1998,33(5):348-350
[32]刘靖华,陈惠孙,胡德耀.人参皂苷对创伤失血性休克免疫功能的调节作用.第三军医大学学报.2000,22(4):324
[33]蒋艳,王毅,邱全瑛.人参皂甙Rg1及其肠内菌代谢产物Rh1对小鼠免疫细胞功能的影响[J].中国病理生理杂志.2001,17(8):815
[34]沈玲,张均田,刘态.人参增强免疫研究新进展.中草药.1996,27(8):499-502
[35]张仲苗,江波,章荣华等.人参皂甙Rg3对小鼠免疫功能的影响.中药药理与临床.2004,20(6):4-6
[36]胡松华,林锋强.人参皂甙Rb1的免疫佐剂作用[J].中国兽医学报.2003,23(5): 480-482
[37] Bancherv J , Steinmen RM. Dendritric cell and the control .immunity. Nature. 1998,392(6673):245
[38]龙振洲.医学免疫学.北京:人民卫生出版社,1999:105
[39]王毅,郝钰,邱全瑛.人参皂苷Rg1及Rh1对树突状细胞刺激T细胞增殖及LPAK抗肿瘤活性的影响.中国免疫学杂志.2003,19(4):248-252
[40]龙振洲.医学免疫学.北京:人民卫生出版社,1999:100
[41]郭志廷,韦旭斌,梁剑平等.人参总皂苷及其衍生物对小鼠淋巴细胞和NK细胞免疫活性的影响.中国兽医学报.2007,27(5):715-717
[42] Yang Guizhen ,Yu Yongli.effects of ginsenoside on the natural killer cell-interferon-interleukin-2 regulatory network and its tumor inhibiting effect.Journal of Traditional Chinese Medicine.1988,8(2):135
[43]张远强.人参对巨噬细胞影响的定量细胞化学研究等.病理生理学报.1985,1(4):22
[44] Xiaoguang C, Hongyan L, Xiaohong L, et al Cancer cheomp reventive and therapeutic acivities of red ginseng[ J ]. J Ethnopharm acol.1998,60(1):71 -78
[45] Kang K S, Kang B C,Lee B J, et al. Preventive effect of ep icatechin and ginsenoside Rb(2) on the inhibition of gap juctional inter, cellular communication by TPA and H2O2 [ J ] Cancer Lett.2000,152(1):97-106
[46] Wargovich M J. Colon cancer chemop revention with ginseng and other botanicals[J]. J Korean Med Sci.2001,16(l): 81-86
[47] Xie X,Eberding A ,Madera C ,Fazli L ,Jia W,Goldenberg L ,et al . Rh2 Synergistically Enhances Paclitaxel or Mitoxantrone in Prostate Cancer Models[J] The Journal of Urology.2006,175 (5):26–31
[48]陈明伟,姚昱,石志红.人参皂甙Rg3联合化疗对30例非小细胞肺癌的近期疗效观察[J].华南国防医学杂志.2005,19(1):4-6
[49] Suda K, Murakami K, Murata J, et a1.An intestinal bacterial metabolite of ginseng protopanaxadiol saponins inhibits tumot–induced neovascularization[J].J. Trad. Med. 2000,17:144-150
[50]张有为,窦德强,陈英杰等.人参皂甙对人体骨肉瘤细胞U2OS增殖的影响[ J ].中草药. 2001,32(3):232-236
[51]葛迎春,刘平,韩信昌.人参皂Rb1, Rg1, Re和Rh1对Hela细胞的影响[ J ].中药理与临床.1997,13(6):18-20
[52] Kim, H E Oh J H, Lee S, K, et al. Ginsenoside Rh2 induces apop totic cell death in rat C6 glioma via a reactive oxygen-and caspase-dependent but Bcl-X(L)-independent pathway[J]. L ife Sci,1999,65(3):33-40
[53]凌昌全,俞超琴,潘瑞萍等.人参皂甙对人T淋巴细胞白血病细胞株凋亡基因的影响[J].中医杂志.2000,41(3):213-215
[54]王占峰,罗毅男,洪新雨等.人参皂甙Rh2抗肿瘤作用机制的研究[J].北华大学学报.2005,6(1):50-52
[55] Mochizuki M, Yung CY, Kaori M ,et al.Inhibition effect of tumor metastasis in mice by saponins, ginsenoside-Rb2,20(R)- and 20(S) ginsenoside - Rg3, of red ginseng [J]. Biol. Pharm. Bull.1996,18(9):1197-1202
[56] Hasegawa H, Uchiyama M.Antimetasatic efficacy of orally administered girtsenoside Rb1 in dependence no intestinal bacterial hydrolyzing potential and significance of treatment with an active hacterial metabolite[J].Planta. Med. 1998,64:696-700
[57]黄宗锈,陈冠敏,林蔚.人参皂甙延缓衰老的实验研究[J].实用预防医学. 2001,8(5):385
[58]龚国清.人参皂甙等清除超氧阴离子自由基的研究.中国药科大学学报.1991,(1):41-43
[59] Chang M S, Choi K J,Rho H M.PD.人参皂甙诱导自由基排除效果.高丽人参学会杂志.1999,23(l):44-49 [60 ] Wee J J,Hco J N,kim M W,Kang D Y.红参的过氧化防御作用.高丽人参学会杂志.1996,20(2):154-155
[61]韩红梅,杨贵贞.人参三醇型皂甙调整骨髓受抑模型鼠的免疫功能.中国免疫学杂志.1991,7(5):312-316
[62]柳忠辉,王树和,杨贵贞.人参皂甙对外源ACTH所致小鼠免疫功能抑制的调整效应.白求恩医科大学学报.1995,21(4):350-352
[63]宋承吉.对《古代人参基源再认识》的质疑.人参研究.1998,(3):17
[64]张语迟,1王淑敏,宋凤瑞等.市售高丽参与中国红参的质量评价.长春中医药大学学报.2008.24(2): 148-149
[65]龙振洲.医学免疫学.北京:人民卫生出版社.1999:51
[66]杜念兴.兽医免疫学[M] .上海:上海科技出版社.1984:272-274
[67]龙振洲.医学免疫学.北京:人民卫生出版社.1999:131