利用VIGS技术研究棉花抗逆基因功能
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摘要
棉花(Gossypium spp.)是重要的经济作物之一。生物和非生物胁迫对棉花产量和棉纤维品质造成严重影响,揭示棉花抗逆的分子机制成为研究棉花的重要目标。本研究利用病毒诱导的基因沉默技术(Virus Induce Gene Silencing, VIGS)和转录组测序(RNA-seq)技术,揭示了GhCPS和GhKS参与DPC的调控机制,是DPC作用的靶位点；GhOST1与GhMPK6相互磷酸化正向调控ABA依赖型信号通路；GhBAK1在抗黄萎病机制和细胞死亡中具有正调控作用；成功构建了棉花VIGS cDNA文库。研究结果如下：
1.本研究采用同源克隆技术获得了棉花的古巴焦磷酸合酶(Copaly1Pyrophosphate Synthase,CPS)基因GhCPS和内根-贝壳杉合成酶(Ent-Kaurene Synthase,KS)基因GhKS,利用VIGS技术对GhCPS和GhKS的功能进行研究。GhCPS基因全长为2466bp,包含保守的DXDD和SAYDTAW基序与其他物种中CPS的氨基酸序列同源性达50%,GhCPS与PtCPS有较近的亲缘关系；3hKS基因全长为2343bp,包含保守的DDXXD和YDTAWVA基序与其他物种中KS的氨基酸序列同源性达50%,GhKS与CsKS有较近的亲缘关系；DPC处理抑制了GhCPS和GhKS的转录水平；VIGS获得了GhCPS和GhKS功能缺失的棉花突变体植株,其节间伸长受到明显抑制；GA3处理后棉花节间生长恢复,DPC失去抑制作用。
2.本研究利用转录组测序技术分析干旱胁迫下棉花的转录组变化。2天干旱处理有102个基因上调表达,4天干旱处理有169个基因上调表达,2天和4天干旱处理同时诱导25个基因上调表达；半定量RT-PCR验证了部分上调基因的转录水平。
3.利用VIGS技术鉴定出了两个参与干旱胁迫的基因GhOST1和GhWRKY30. VIGS-GhOST1或VIGS-GhWRKY30的植株对干旱敏感且表型稳定,相对含水量与对照相比显著性下降；VIGS-GhWRKY30的植株对PEG胁迫敏感,对NaCl不敏感；VIGS-GhOST1的植株对PEG和NaCl胁迫均敏感。
4.本研究利用体外试验分析GhMPK6与GhOST1两个蛋白激酶之间的互作。免疫共沉淀(Co-Immunoprecipitation, Co-IP)和Pull-Down试验结果证明脱落酸(Abscisic acid, ABA)可诱导GhMPK6与GhOST1的互作；体外激酶活性测定结果表明两者之间能够发生磷酸化转移。
5.本研究利用同源比对和VIGS技术分析棉花GhBAK1的基因功能。同源比对分析发现棉花中有2个BAK1的同源基因；VIGS结果表明GhBAK1是棉花抗黄萎病所必须的组分；台盼蓝染色和DAB染色结果显示GhBAK1缺失后棉花叶片细胞死亡,活性氧含量急剧增加。
6.本研究成功构建了二倍体棉花的cDNA文库。该文库包括2×106个克隆,库容是棉花基因组的5-10倍；PCR、双酶切鉴定和基因测序结果表明单克隆中插入的片段大小集中在0.5-1Kb之间,且功能各异。
Cotton (Gossypium spp.) serves as a economically important crop. However, cotton production is hindered by various biotic and abiotic stresses. The genetic and molecular mechanisms mediating cotton stress responses remain poorly understood. By using RNA-seq and VIGS approach, this studies uncovered that DPC regulated cotton growth via GhCPS and GhKS which were the possible functional sites; GhOST1and GhMPK6could phosphorylate each other to regulate ABA-dependent signaling pathway positively; GhBAK1play positive role in Verticillium wilt resistance and cell death; Cotton VIGS cDNA library was constructed to dissection genes.
1. GhCPS and GhKS genes were cloned by homology cloning strategy. The genetic Function of those two was claimed by VIGS. The full length of GhCPS including two conserved domains DXDD and SAYDTAW was2466bp, which had50%amino acid similarity with other species CPS by homology analysis. Phylogenetic analysis indicated GhCPS and PtCPS belonged to the same clade; the full length of GhKS including two conserved domains DDXXD and YDTAWVA was2343bp, which had50%amino acid similarity with other species KS by homology analysis. Phylogenetic analysis indicated GhKS and CsKS belonged to the same clade; DPC treatment could decrease the transcriptional level of GhCPS and GhKS. By loss-of-function analysis, our studies showed that the elongation of cotton internode was inhibited by VIGS-GhCPS or VIGS-GhKS. The inhibition could compromise by GA3treatment.
2. This study analysized the transcripton change of cotton upon drought treatment with RNA-seq approach.102and169genes up-regulated by2days or4days drought stress were identified.25genes up-regulated by both2days and4days drought stress; Part of those up-regulated genes were confirmed by semi-quantitative RT-PCR.
3. Two genes GhOSTl and GhWRKY30involving in drought tolerance were identified by VIGS. Cotton seedlings of VIGS-GhOSTl or VIGS-GhWRKY30were sensitive to drought. Water lost in cotton leaves of VIGS-GhOST1or VIGS-GhWRKY30were significantly increased; and relative water content of leaves were significantly decreased; cotton VIGS-GhWRKY30were sensitive to PEG but not NaCl; cotton VIGS-GhOST1were sensitive to both treatment.
4. This study identified the interaction of two protein kinase GhOST1and GhMPK6by in vitro assay. Co-IP and Pull down assay indicated that ABA could induce the interaction between GhOST1and GhMPK6; In vitro kinase assay indicated that there is a phosphorylation event happened between GhOST1and GhMPK6.
5. This study analysized the function of GhBAKl by homology blastn and VIGS. Two BAK1 orthologs were identified in the cotton genome; this study indicated GhBAK1is required for CA4002resistancing to Verticillium wilt by loss-of-function analysis; silencing of GhBAK1is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton.
6. A detailed protocol of construction of a VIGS library from diploid cotton G raimondii was established. This library contained2×106clones; sequencing of the representative colonies revealed that the library covers a significant percentage of cotton unique genes; based on the number of colonies in the primary library, it is estimated that this VIGS library is about50×coverage of predicted cotton protein-coding genes; each clone contained diverse gene fragment.
1.本研究采用同源克隆技术获得了棉花的古巴焦磷酸合酶(Copaly1Pyrophosphate Synthase,CPS)基因GhCPS和内根-贝壳杉合成酶(Ent-Kaurene Synthase,KS)基因GhKS,利用VIGS技术对GhCPS和GhKS的功能进行研究。GhCPS基因全长为2466bp,包含保守的DXDD和SAYDTAW基序与其他物种中CPS的氨基酸序列同源性达50%,GhCPS与PtCPS有较近的亲缘关系；3hKS基因全长为2343bp,包含保守的DDXXD和YDTAWVA基序与其他物种中KS的氨基酸序列同源性达50%,GhKS与CsKS有较近的亲缘关系；DPC处理抑制了GhCPS和GhKS的转录水平；VIGS获得了GhCPS和GhKS功能缺失的棉花突变体植株,其节间伸长受到明显抑制；GA3处理后棉花节间生长恢复,DPC失去抑制作用。
2.本研究利用转录组测序技术分析干旱胁迫下棉花的转录组变化。2天干旱处理有102个基因上调表达,4天干旱处理有169个基因上调表达,2天和4天干旱处理同时诱导25个基因上调表达；半定量RT-PCR验证了部分上调基因的转录水平。
3.利用VIGS技术鉴定出了两个参与干旱胁迫的基因GhOST1和GhWRKY30. VIGS-GhOST1或VIGS-GhWRKY30的植株对干旱敏感且表型稳定,相对含水量与对照相比显著性下降；VIGS-GhWRKY30的植株对PEG胁迫敏感,对NaCl不敏感；VIGS-GhOST1的植株对PEG和NaCl胁迫均敏感。
4.本研究利用体外试验分析GhMPK6与GhOST1两个蛋白激酶之间的互作。免疫共沉淀(Co-Immunoprecipitation, Co-IP)和Pull-Down试验结果证明脱落酸(Abscisic acid, ABA)可诱导GhMPK6与GhOST1的互作；体外激酶活性测定结果表明两者之间能够发生磷酸化转移。
5.本研究利用同源比对和VIGS技术分析棉花GhBAK1的基因功能。同源比对分析发现棉花中有2个BAK1的同源基因；VIGS结果表明GhBAK1是棉花抗黄萎病所必须的组分；台盼蓝染色和DAB染色结果显示GhBAK1缺失后棉花叶片细胞死亡,活性氧含量急剧增加。
6.本研究成功构建了二倍体棉花的cDNA文库。该文库包括2×106个克隆,库容是棉花基因组的5-10倍；PCR、双酶切鉴定和基因测序结果表明单克隆中插入的片段大小集中在0.5-1Kb之间,且功能各异。
Cotton (Gossypium spp.) serves as a economically important crop. However, cotton production is hindered by various biotic and abiotic stresses. The genetic and molecular mechanisms mediating cotton stress responses remain poorly understood. By using RNA-seq and VIGS approach, this studies uncovered that DPC regulated cotton growth via GhCPS and GhKS which were the possible functional sites; GhOST1and GhMPK6could phosphorylate each other to regulate ABA-dependent signaling pathway positively; GhBAK1play positive role in Verticillium wilt resistance and cell death; Cotton VIGS cDNA library was constructed to dissection genes.
1. GhCPS and GhKS genes were cloned by homology cloning strategy. The genetic Function of those two was claimed by VIGS. The full length of GhCPS including two conserved domains DXDD and SAYDTAW was2466bp, which had50%amino acid similarity with other species CPS by homology analysis. Phylogenetic analysis indicated GhCPS and PtCPS belonged to the same clade; the full length of GhKS including two conserved domains DDXXD and YDTAWVA was2343bp, which had50%amino acid similarity with other species KS by homology analysis. Phylogenetic analysis indicated GhKS and CsKS belonged to the same clade; DPC treatment could decrease the transcriptional level of GhCPS and GhKS. By loss-of-function analysis, our studies showed that the elongation of cotton internode was inhibited by VIGS-GhCPS or VIGS-GhKS. The inhibition could compromise by GA3treatment.
2. This study analysized the transcripton change of cotton upon drought treatment with RNA-seq approach.102and169genes up-regulated by2days or4days drought stress were identified.25genes up-regulated by both2days and4days drought stress; Part of those up-regulated genes were confirmed by semi-quantitative RT-PCR.
3. Two genes GhOSTl and GhWRKY30involving in drought tolerance were identified by VIGS. Cotton seedlings of VIGS-GhOSTl or VIGS-GhWRKY30were sensitive to drought. Water lost in cotton leaves of VIGS-GhOST1or VIGS-GhWRKY30were significantly increased; and relative water content of leaves were significantly decreased; cotton VIGS-GhWRKY30were sensitive to PEG but not NaCl; cotton VIGS-GhOST1were sensitive to both treatment.
4. This study identified the interaction of two protein kinase GhOST1and GhMPK6by in vitro assay. Co-IP and Pull down assay indicated that ABA could induce the interaction between GhOST1and GhMPK6; In vitro kinase assay indicated that there is a phosphorylation event happened between GhOST1and GhMPK6.
5. This study analysized the function of GhBAKl by homology blastn and VIGS. Two BAK1 orthologs were identified in the cotton genome; this study indicated GhBAK1is required for CA4002resistancing to Verticillium wilt by loss-of-function analysis; silencing of GhBAK1is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton.
6. A detailed protocol of construction of a VIGS library from diploid cotton G raimondii was established. This library contained2×106clones; sequencing of the representative colonies revealed that the library covers a significant percentage of cotton unique genes; based on the number of colonies in the primary library, it is estimated that this VIGS library is about50×coverage of predicted cotton protein-coding genes; each clone contained diverse gene fragment.
引文
1. Abuqamar S, Chen X, Dhawan R, et al. Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J.,2006, 48:28-44.
2. Adams S S, Rouse D I, Bowden R L. Performance of alternative versions of POTWIL:a computer model that simulates the seasonal growth of Verticillium infected potato. Am Potato J.,1987,64: 429-435.
3. Ait A T, Swain S M. The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. Plant J.,1997,11:443-454.
4. Anderson J V, Chevone B I, Hess J L. Seasonal variation in the antioxidant systerm of eastern white pine needles. Plant Phy,1992,98:501-508.
5. Andrew L P. Gibberellins in Arabidopsis. Plant Physiol Biochem.,1998,36:115-124.
6. Anjum F, Yaseen M, Rasul E, et al. Water stress in barley (Hordeum vulgare L.). I. Effect on chemical composition and chlorophyll contents. Pakistan J. Agr. Sci.2003,40:45-49.
7. Anna C M, Sylvain M, Alain V, et al. Arabidopsis OST1 Protein Kinase Mediates the Regulation of Stomatal Aperture by Abscisic Acid and Acts Upstream of Reactive Oxygen Species Production. Plant Cell,2002,14(12):3089-3099.
8. Annette B and Matthias L. VIGS-genomic goes functional. Trends in Plant Sci,2009,15(1):1-4.
9. Asai T, Tena G, Plotnikova J, et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature,2002.415:977-983.
10. Au K F, Jiang H, Lin L, et al. Detection of splice junctions from paired-end RNA-seq data by SpliceMap. Nucleic Acids Res,2010,38(14):4570-4578.
11. Ausubel F M. Are innate immune signaling pathways in plants and animals conserved? Nat Immunol,2005,6:973-979.
12. Baulcombe D C. Fast forward genetics based on virus-induced gene silencing. Curr Opin in Plant Biol,1999,2:109-113.
13. Baulcombe D C. RNA silencing in plants. Nature,2004,431 (7006):356-363.
14. Becker A, Lange M. VIGS-genomics goes functional. Trends Plant Sci,2010,15:1-4.
15. Bensen R J, Johal G S. Clonging and characterization of the maize Anl gene. Plant Cell,1995,7: 75-84.
16. Bergmann D C, Lukowitz W, Somerville C R. Stomatal development and pattern controlled by a MAPKK kinase. Science,2004,304:1494-1497.
17. Boller T, Felix G A renaissance of elicitors:perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol,2009,60:379-406.
18. Bota J, Flexas J, Medrano H. Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? New Phytol,2004,162:671-681.
19. Boudsocq M, Barbier-Brygoo H, Lauriere C. Identification of nine sucrose nonfermenting related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem,2004,279(40):41758-41766.
20. Burton R A, Gibeaut D M, Bacie A, et al. Virus-induced silencing of a plant cellulose synthase gene. Plant cell,2000,12:691-705.
21. Cathey H M. Physiology of growth retarding chemicals. Annu Rev Plant Physiol.,1964,15: 271-302.
22. Cogoni C, Macino G. Post-transcriptional gene silencing across kingdoms. Curr Opin Genet Dev., 2000,10(6):638-643.
23. Colcombet J, Boisson-Dernier A, Ros-Palau R, et al. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 are essential for tapetum development and microspore maturation. Plant Cell,2005,17:3350-3361.
24. Cornic G, Massacci A. Leaf photosynthesis under drought stress, in:Baker N.R., (Ed.), Photosynthesis and the Environment, Kluwer Academic Publishers, The Netherlands.1996.
25. Chen L, Zhang L, Yu D. Wounding-induced WRKY8 is involved in basal defense in Arabidopsis. Mol Plant-Microbe Interact,2010,23:558-565.
26. Chen Z J, Scheffler B E, Dennis E, et al. Toward sequencing cotton(Gossypium) genomes. Plant Physiol.,2007,145:1303-1310.
27. Chinchilla D, Shan L, He P, et al. One for all:the receptor-associated kinase BAK1. Trends Plant Sci.,2009.14:535-541.
28. Chinchilla D, Zipfel C, Robatzek S, et al. Flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature,2007,448:497-500.
29. Cho K Y, Sakurai A, Kamiya Y, et al. Effects of the new plant growth retardants of quaternary ammonium iodides on gibberellin biosynthesis in Gibberella fujikuroi. Plant Cell Physiol.,1979, 20:75-81.
30. Choi H I, Park H J, Park J H, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol.,2005,139:1750-1761.
31. Cutler S R, Rodriguez P L, Finkelstein R R, et al. Abscisic acid:Emergence of a core signaling network. Annu Rev Plant Biol.,2010,61:651-679.
32. Dawson W O. Gene silencing and virus resistance:a common mechanism. Trends Plant Sci.,1996, 1:107-108.
33. DeLucia E H, Heckathorn S A. The effect of soil drought on water-use efficiency in a contrasting Great Basin desert and Sierran montane species. Plant Cell Environ,1989,12:935-940.
34. Dever J, Wheeler T, Kelly C. Registration of CA 4002 cotton germplasm line partially resistant to Verticillium wilt. Journal of Plant Regist.,2013,7:1-7.
35. Dicks J W. Mode of action of growth retardants. See Ref.25a,1980, pp:1-14.
36. Dinesh-Kumar S P, Anandalakshmi R., Marathe R, et al. Virus-induced gene silencing. Methods Mol Biol.,2003,236:287-294.
37. Ding X S, Rao C S, Nelson RS. Analysis of gene function in rice through virus-induced gene silencing. Methods Mol Biol.,2007,354:145-160.
38. Ding X S, Schneider W L, Chaluvadi S R, et al. Characterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts. Mol Plant Microbe Interact., 2006,19:1229-1239.
39. Dong J, Chen C, Chen Z. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol.,2003,51:21-37.
40. Edgren H, Murumagi A, Kangaspeska S, et al. Identification of fusion genes in breast cancer by paired-end RNA-sequencing. Genome Biol.,2011,12(1):R6.
41. Egilla J N, Davies Jr F T, Boutton T W. Drought stress influences leaf water content, photosynthesis, and water-use efficiency of Hibiscus rosa-sinensis at three potassium concentrations. Photosynthetica.,2005,43:135-140.
42. Eulgem T, Rushton P J, Robatzek S, et al. The WRKY superfamily of plant transcription factors. Trends Plant Sci.,2000,5:199-206.
43. Eynck C, Koopmann B, Grunewald-Stoecker G, et al. Differential interactions of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques. Eur J Plant Pathol.,2007,118(3):259-274.
44. Fahnenstich H, Scarpeci T E, Valle E M, et al. Generation of hydrogen peroxide in chloroplasts of Arabidopsis overexpressing glycolate oxidase as an inducible system to study oxidative stress. Plant Physiol.,2008,148:719-729.
45. Farooq M, Wahid A, Kobayashhi N, et al. Plant drought stress:effects, mechanisms and management. Agronomy,2009,29:185-212.
46. Filichkin S A, Priest H D, Givan S A, et al. Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Res.,2010,20(1):45.
47. Finkelstein R, Gampala S S, Lynch T J, et al. Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3. Plant Mol. Biol.,2005,59:253-267.
48. Fradin E F, Thomma B P. Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol Plant Pathol.,2006,7:71-86.
49. Fradin E F, Zhang Z, Juarez Ayala J C, et al. Genetic dissection of Verticillium wilt resistance mediated by tomato Vel. Plant Physiol.,2009,150:320-332.
50. Fu D Q, Zhu B Z, Zhu H L, et al. Eahancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol. Cell,2006,1:153-160.
51. Fu D Q, Zhu B Z, Zhu H L, et al. Vims-induced gene silencing in tomato fruit. Plant J.,2005,43: 99-308.
52. Fu J, Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot.,2001,45:105-114.
53. Fujii H, Verslues P E, Zhu J K. Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell,2007, 19(2):485-494.
54. Fujii H, Verslues P E, Zhu J K. Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo. Proc. Natl. Acad. Sci. USA,2011,108(4):1717-1722.
55. Fujii H, Zhu J K. Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. Proc. Natl. Acad. Sci. USA,2009,106(20): 8380-8385.
56. Fujii H, Zhu J K. Osmotic stress signaling via protein kinases. Cell Mol. Life Sci.,2012, 69(19):3165-3173.
57. Fujita Y, Nakashima K, Yoshida T, et al. Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol., 2009,50:2123-2132.
58. Gao M, Wang X, Wang D, et al. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Micro.,2009,6:34-44.
59. Gao X, Britt R C, Shan L, et al. Agrobacterium-mediated virus-induced gene silencing assay in cotton. J Vis Exp.,2011a.
60. Gao X, Shan L. Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing. Methods Mol. Biol.,2013,975:157-165.
61. Gao X, Wheeler T, Li Z, et al. Silencing GhNDRl and GhMKK2 compromises cotton resistance to Verticillium wilt. Plant J.,2011b,66:293-305.
62. Garrod J F, Hewitt H G, Copping L G, et al. A new group of ternary sulphonium growth retardants. See Ref.25a,1980, pp:67-74.
63. Gossmann K. Plant growth retardants:their mode of action and benefit for physiological research. See Ref.93a,1992, pp:788-797.
64. Gou X, Yin H, He K, et al. Genetic evidence for an indispensable role of somatic embryogenesis receptor kinases in brassinosteroid signaling. PLoS Genet.,2012,1002452.
65. Hamilton A J, Baulcombe D C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science,1999,286:950-952.
66. Hamilton A J, Bouzayen M, Grierson D. Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast. Proc. Natl. Acad. Sci. U S,1991,88(16):7434-7437.
67. Hashmi J A, Zafar Y, Arshad M, et al. Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes,2011,42: 286-296.
68. Hayward A, Padmanabhan M, Dinesh-Kumar S P. Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species. Plant Reverse Genetics:Methods and Protocols, Methods in Molecular Biology,2011,678:55-63.
69. He K, Gou X, Yuan T, et al. BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr. Biol.,2007,17:1109-1115.
70. Hecht V, Vielle-Calzada J P, Hartog M V, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol.,2001,127:803-816.
71. Hedden P. The action of plant growth retardants at the biochemical level. See Ref.132a,1990, pp: 322-332.
72. Heese A, Hann D R, Gimenez-Ibanez S, et al. The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc. Natl. Acad. Sci. USA,2007,104:12217-12222.
73. Hinchliffe D J, Meredith W R, Yeater K M, et al. Near-isogenic cotton germplasm lines that differ in fiberbundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling. Theor.Appl. Genet,2010,120:1347-1366.
74. Hoen P A, Ariyurek Y, Thygesen H H, et al. Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray plantform. Nucleic Acids Research,2008,36(21):e141.
75. Hoyos M E, Zhang S. Calcium-independent activation of salicylic acid-induced protein kinase and 40-kDa protein kinase by hyperosmotic stress. Plant Physiol.,2000,122:1355-1363.
76. Huisman O C. Interrelations of Root Growth Dynamics to Epidemiology of Root-Invading Fungi. Annu. Review of Phytopathol.,1982,20:303-327.
77. Idris A M, Tuttle J R, Roberson D, et al. Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physio and Mol Plant Pathol.,2010,75:13-22.
78. Joo J H, Wang S, Chen J, el al. Different signallingand cell death roles of heterotrimeric G protein a and b subunits in the Arabidopsis oxidativestress response to ozone. Plant Cell,2005,17: 957-970.
79. Jostr, Aitschmied L, Bloem E. Expression profiling of metabolic gene in response to methyl jasm on atereveais regulation of genes of primary and secondary sulfur related pathways in Arabidopsis thalinan. Photosynth Research,2005,86:491-508.
80. Jung J. Possibilities for optimization of plant nutrition by new agrochemical substances-especially in cereals. In Plant Regulation and Word Agriculture, ed. TK Scott,1979, pp.279-307. New York: Plenum.
81. Kalde M, Barth M, Somssich I E, et al. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signalling pathways. Mol Plant Microbe Interact,2003, 16:295-305.
82. Kaloshian I. Virus-Induced Gene silencing in plants roots. Plant-Pathogen Interactions:Methods and Protocols. Methods in Molecular Biology,2007,354:173-181.
83. Karlova R, Boeren S, Russinova E, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex includes BRASSINOSTEROID-INSENSITIVE1. Plant Cell,2006,18:626-638.
84. Kawchuk L M, Hachey J, Lynch D R, et al. Tomato Ve disease resistance genes encode cell surface-like receptors. Proc. Natl. Acad. Sci. U S A.,2001,98:6511-6515.
85. Kim K C, Lai Z, Fan B, et al. Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense. Plant Cell,2008,20:2357-2371.
86. Kinoshita T, Cano-Delgado A, Seto H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature,2005,433:167-171.
87. Klingler J P, Batelli G, Zhu J K. ABA receptors:The START of a new paradigm in phytohormone signalling. J Exp Bot.,2010,61(12):3199-3210.
88. Kishor P B K, Hong Z, Miao G H, el al. Over expression of A-pyrroline-5-carboxylate synthetase increases proline products and confers osmo-tolerance in transgenic plants. Plant Physio.,1995, 108:1387-1394.
89. Knoth C, Ringler J, Dangl J L, el al. Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica. Mol Plant Microbe Interact,2007,20:120-128.
90. Koornneef M, and van der Veen J H. Induction and analysis of gibberellin-sensitive mutants in Arabidopsis thaliana (L.) Heynh. Theor. Appl. Genet.,1980,58:257-263.
91. Koornneef M, van Eden J, Hanhart C J, et al. Genetic fine-structure of the GA-7 locus in the higher plant Arabidopsis thaliana (L.) Heynh. Genet. Res. Camb.,1983,41:57-68.
92. Kumagai M H, Donson J, Della-Cioppa G, et al. Cytoplamic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc. Natl. Acad. Sci. USA,1995,92(5):1679-1683.
93. Lacape J M, Claverie M, Vidal R O, et al. Deep sequencing reveals differences in the transcriptional landscapes of fibers from two cultivated species of cotton. PLoS ONE,2012,7(11): e48855. Doi:10.1371/journal. pone.0048855.
94. Lacomme C, Hrubikova K, Hein I. Enhancement of virus-induced gene silencing through viral based production of inverted repeats. Plant J.,2003,34(4):543-553.
95. Lai Z, Li Y, Wang F, et al. Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense. Plant Cell,201 la,23:3824-3841.
96. Lai Z, Wang F, Zheng Z, et al. A critical role of autophagy in plant resistance to necrotrophic fungal pathogens. Plant J.,201lb,66:953-968.
97. Lazaridou M, Koutroubas S D. Drought effect on water use efficiency of berseem clover at various growth stages. New directions for a diverse planet:Proceedings of the 4th International CropScience Congress Brisbane, Australia,2004,26 Sept-1 Oct 2004.
98. Li J, Brader G, Kariola T, et al. WRKY70 modulates the selection of signaling pathways in plant defense. Plant J.,2006,46:477-491.
99. Li J, Brader G, Palva E T. The WRKY70 transcription factor:a node of convergence for jasmonate-mediated and salicylatemediated signals in plant defense. Plant Cell.,2004,16:319-331.
100. Li Z, An L H, Fu Q, et al. Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper. Ecotoxicology,2012,21:260-267.
101. Linser H, Bettner L. Wachstumsretardantien. J. plant Nutr. Soil Sci.,1972,132:105-143.
102. Lindbo J A, Silva-Rosales L, Proebsting W M, et al. Induction of a Highly Specific Antiviral State in Transgenic Plants:Implications for Regulation of Gene Expression and Virus Resistance. Plant cell,1993,5(12):1749-1759.
103. Liu Y, Schiff M, Dinesh-Kumar S P. Involvement of MEK1MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, Col 1 and CRT1 in N-mediated resistance to tobacco mosaic virus. Plant J., 2004,38:800-809.
104. Lu T T, Lu G J, Fan D L, et al. Function an notation of the rice transcriptome at single-nucleotide resolution by RNA-seq. Genome Res.,2010,20(9):1238-1249.
105. Maher C A, Palanisamy N, Brenner J C, et al. Chimeric transcript discovery by paired-end transcriptome sequencing. Proc. Natl. Acad. Sci. USA,2009,106(30):12353-12358.
106. Maqbool A, Abbas W, Rao A Q, et al. Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum. Biotechnol Progr.,2010,26:21-5.
107. Mei C S, Zhou X, Yang Y. Use of RNA interference to dissect defense-signaling pathways in rice. Plant-Pathogen Interactions:Methods and Protocols. Methods in Molecular Biology,2007, vol. 354:161-172.
108. Mitchell J W, Wirwille J W, Weil L. Plant growth-regulating properties of some nicotinium compounds. Science,1949,110:252-254.
109. Monakhova O F, Chernyadev I.I. Protective role of kartolin-4 in wheat plants exposed to soil drought, Appl. Biochem. Micro.,2002,3:373-380.
110. Mortazavi A, Williams B A, McCue K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat. Methods,2008,5(7):621.
111. Muangsan N, Robertson D. Geminivirus vectors for transient gene silencing in plant. Methods Mol. Biol.,2004,265:101-115.
112. Nagalakshmi U, Wang Z, Waem K, et al. The transcriptional landscape of the yeast genome defined by RNA sequencing. Science,2008,320(5881):1344-1349.
113. Nakashima K, et al. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK21/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol.,2009,50(7):1345-1363.
114. Nerd A, Nobel P S. Effects of drought on water relations and nonstructural carbohydrates in cladodes of Opuntia ficus-indica, Physiol. Plant,1991,81:495-500.
115. Nishikura K. A short primer on RNAi:RNA-directed RNA polymerase acts as a key catalyst. Cell, 2001,107(4):415-418.
116. Okoniewski M J, Miller C J. Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations. BMC Bioinformatics,2006,7(1):276. Cell,2001,107(4):415-418.
116. Okoniewski M J, Miller C J. Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations. BMC Bioinformatics,2006,7(1):276.
117. Ozsolak F, Milos PM. RNA sequencing:advances, challenges and opportunities. Nat. Rev. Genet.,2011,12(2):87-98.
118. Pang M X, Percy R G, Stewart J M, et al, Comparative transcriptome analysis of Pima and Acala cotton during boll development using 454 pyrosequencing technology. Molecular Breeding,2012, 30(2):1143-1152.
119. Peuke A D, Hartung W, Schraml C, et al. Identification of drought sensitive beech ecotypes by physiological parameters, New Phytol.,2002,154:373-388.
120. Pflieger S, Blanchet S, Camborde L, et al. Efficient virus-induced gene silencing in Arabidopsis using a'one-step'TYMV-derived vector. Plant J.,2008,56:678-690.
121. Qiu J L, Zhou L, Yun B W, et al. Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1. Plant Physiol.,2008,148:212-222.
122. Qu J, Ye J, Geng Y F, et al. Dissecting functions of KATANIN and WRINKLED 1 in cotton fiber development by virus-induced gene silencing. Plant Physiol.,2012,160:738-748.
123. Rademacher W. Biochemical effects of plant growth retardants. See Ref.49a,1991, pp.169-200
124. Rao A Q, Bakhsh A, Nasir I A, et al. Phytochrome B mRNA expression enhances biomass yield and physiology of cotton plants. Afr. J. Biotechnol.,2011,10:1818-26.
125. Ratcliff F, Martin-Hernandez A M, Baulcombe D C. Tobacco rattle virus as a vector for analysis of gene function by silencing. The Plant journal,2001,25(2):237-245.
126. Ratcliff F, MacFarlane S, Baulcombe D C. Gene silencing without DNA:RNA-mediated cross protection between viruses. Plant Cell,1999,11:1207-1215.
127. Ravi V M, Yao Liu, Tracy R R, et al. The tomato Fni3 Lysine-63-specific ubiquitin-conjugating enzyme and Suv ubiquitin E2 Variant positively regulate plant immunity. Plan Cell,2013,25: 3615-3631.
128. Ren B Z. Biochemistry and Clinical medicine. Chmlgsha, Hunan Sience and Technical Press,1993.
129. Richman A S, Gijzen M. Diterpene synthesisin Stevia rebaudiana:recruitment and upregulation of key enzymes from the gibberellin biosynthetic pathway. Plant J.,1999,19:411-421.
130. Roux M, Schwessinger B, Albrecht C, et al. The Arabidopsis leucine-rich repeat receptor-like kinases BAK.1/SERK3 and BKK1/SERK4 are required for innate immunity to hemibiotrophic and biotrophic pathogens. Plant Cell,2011,23:2440-2455.
131. Royce T E, Rozowsky J S, Gerstein M B. Toward a universal microarray:prediction of gene expression through nearest-neighbor probe sequence identification. Nucleic Acids Res.,2007, 35(15).
132. Ruiz M T, Voinnet, Baulcombe D C. Initiation and maintenance of virus-induced gene silencing. Plant cell,1998,10(6):937-946.
133. Samuel M A, Miles G P, Ellis B. Ozone treatment rapidly activates MAP kinase signalling in plants. Plant J.,2000,22:367-376.
134. Sauter H. Chemical aspects of some bioregulators. In Bioregulators:Chemistry and Uses, ed. RL Ory, FR Rittig,1984, pp.9-21. Washington, DC:Am. Chem. Soc.
135. Scofield S R, Huang L, Brandt A S, et al. Development of a virus induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol.,2005,138:2165-2173.
136. Schmidt E D, Guzzo F, Toonen M A, et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development,1997,124:2049-2062.
137. Schulze B, Mentzel T, Jehle A K, et al. Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1. J. Biol. Chem., 2010,285:9444-9451.
138. Shan L, He P, Li J, et al. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Micro., 2008,4:17-27.
139. Shan L, He P, Sheen J. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host Microb.,2007,1:167-174.
140. Sharkey T D. Water stress effects on photosynthesis. Photosynthetica,1990,24,651-661.
141. Shendue J. The beginning of the end for microarrays? Nature Methods,2008,5(7):585-587.
142. Shiu S H, Bleecker A B. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc. Natl. Acad. Sci. USA,2001,98:10763-10768.
143. Shiu S H, Bleecker A B. Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis. Plant Physiol.,2003,132:530-543.
144. Smith M S, Yamaguchi S, Ait-Ali, et al. The first step of gibberellin biosynthesis in pumpkin is catalyzed by at least two copalyl diphosphate synthases encoded by differentially regulated genes. Plant Physiol.,1998,118:1411-1419.
145. Smith T M, Anderson J C, Martin G B, et al. Applications and advantages of virus-induced gene silencing for gene function studies in plant. Plant J,2004,39(5):734-746.
146. Smith T M, Schiff M, Liu Y, et al. Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol,2006,142(1):21-7.
147. Sultan M, Schulz M H, Richard H, et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science,2008,321(5891):956.
148. Sun T-p, Goodman H M, Ausubel F M. Cloning the Arabidopsis GA1 locus by genomic subtraction. Plant Cell,1992,4:119-128.
149. Sunilkumar G, Campbell L M, Puckhaber L, et al. Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc. Natl. Acad. Sci. USA,2006,103: 18054-18059.
150. Takeshi N, Jung-Bum L. cDNA clonging, functional expression and characterization of ent-Copalyl Diphosphate Synthases from Scoparia dulcis L. Plant Science,2005,169:760-767.
151. Tallys P W. Association of tylosis and hyperplasia of the xylem with vascular invasion of the hop by Verticillium alboatrum. Transactions British Mycological Society TALBOYS,1958,41: 249-260.
152. Telma E S, Man'a I Z, Bernd M R, et al. Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana. Plant Mol. Biol.,2013,83:265-277.
153. Tezara W, Mitchell V J, Driscoll S D, et al. Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. Nature,1999,401:914-917.
154. Thomas C L, Jones L, Baulcombe D C, et al. Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. Plant J.,2001,25(4):417-425. substance. I. Chemical structure and bioassay. J. Biol. Chem.,1960a,235:475-479.
157. Tolbert N E. (2-Chloroethyl)-trimethylammoniumchloride and related compounds as plant growth substance. II. Effect on growth of wheat. Plant Physiol.,1960b,35:380-385.
158. Tomonobu T, Takuma K. Clonging and characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat:Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes. Biosci. Biotechnol. Biochem.,2009,73(3):772-775.
159. Travella S, Klimm T E, Keller B. RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat. Plant Physiol,2006.142(1):6-20.
160. Tuttle J R, Idris A M, Brown J K, et al. Geminivirus mediated gene silencing from Cotton leaf crumple virus is enhanced by low temperature in cotton. Plant Physiol.,2008,148:41-50.
161. Van Kammen A. Virus-induced gene silencing in infected and transgenic plants. Trends plant Sci., 1997,2:409.
162. Voinnet O. RNA silencing as a plant immune system against viruses. Trends Genet.,2001,17:449.
163. Vu J C V, Gesch R W, Allen L H, et al. CO2 enrichment delays a rapid, drought-induced decrease in Rubisco small subunit transcript abundance, J. Plant Physiol.,1999,155:139-142.
164. Wahid A, Rasul E. Photosynthesis in leaf, stem, flower and fruit, in:Pessarakli M. (Ed.), Handbook of Photosynthesis,2nd ed., CRC Press, Florida,2005, pp.479-497.
165. Wang G D, Ellendorff U, Kemp B, et al. Genome-wide functional investigation into the roles of receptor-like proteins in Arabidopsis. Plant physiol.,2008,147:503-517.
166. Wang K, Wang Z, Li F, et al. The draft genome of a diploid cotton Gossypium raimondii. Nat Genet.,2012,44:1098-1103.
167. Wang P, Liang X, Giorgia B, et al. Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action. PNAS,2013,110:11205-11210.
168. Waterhouse P M, Helliwell C A. Exploring plant genomes by RNA-induced gene silencing. Nature Rev Genet.,2003,4(1):29-38.
169. Wendel J F. Genome evolution in polyploids. Plant Mol Biol.,2000,42:225-249.
170. Wesley S V, Helliwell C A, Smith N A, et al. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J.,2001,27(6):581-590.
171. Wettstein D V, Gough S, Kannangara C G. Chlorophyll biosynthesis. Plant Cell,1995,7: 1039-1057.
172. Wilhelm B T, Marguerat S, Watt S, et al. Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution. Nature,2008,453(7199):1239-1243.
173. Wilhelm R. Growth retardants:Effects on Gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol. Plant Mol. Biol.,2000,51:501-531.
174. Xiang L X, He D, Dong W R, et al. Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marinefish. BMC Genomaics,2010,11(1):472.
175. Xu M, Ross W. Functional characterization of the rice kaurene synthase-like gene family. Phytochem.,2007,68:312-326.
176. Yamaguchi S, Sun T-p, Kawaide H, et al. The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant Physiol.,1998b,116:1271-1278.
177. Ye B, Gressel J. Transient oxidant-induced antioxigant transcript and enzyme levels correlate with greater oxidant-resistance in paraquant-resistant Conyza bonariensis. Planta,2000,211:50-61.
178. Yokota A, Kawasaki S, Iwano M, et al. Citrulline and DRIP-1 Protein (ArgE Homologue) in Drought Tolerance of Wild Watermelon. Ann. Bot.,2002,89:825-832.
179. Yoshida T, Fujita Y, Sayama H, et al. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J.,2010,61:672-685.
180. Yu D, Chen C, Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell,2001,13:1527-1540.
181. Yu J, Kohel R J, Smith C W. The construction of a tetraploid cotton genome wide comprehensive reference map. Genomics.,2010,95:230-240.
182. Yue Y, Zhang M, Zhang J, et al. Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton. J Exp Bot.,2012,63(10):3741-3748.
183. Zeeh B, Konig K H, Jung J. Development of a new plant growth regulator with biolodical activity related to CCC. Kemia(Helsinki),1974,9:621-623.
184. Zhang G J, Guo G W, Hu X D, et al. Deep RNA sequencing at single base-pair resolution reveals high complexity of the rice transcriptome. Genome Res.,2010,20(5):646-654.
185. Zhang H, Shen G X, Kuppu S, et al. Creating drought-and salt-tolerant cotton by overexpressing a vacuolar pyrophosphatase gene. Plant Signaling&Behavior,2011,6 (6):861-863.
186. Zhang H B, Li Y, Wang B, and Chee P W. Recent advances in cotton genomics. Int. J. Plant Genomics,2008,742304.
187. Zheng Z, Qamar S A, Chen Z, et al. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J.,2006,48:592-605.
188. Zhong W, Mark G, Michael S. Applications of next-generation sequencing. Nature Reviews Genetics,2009,10:57-63.
189. Zhu J K. Salt and drought stress signal transduction in plants. Ann. Rev. Plant Bio.,2002,53: 247-273.
190.白克智.植物生长调节剂实用问答.北京：化学工业出版社,1998,16.
191.陈峰,李洁.酵母单杂交的原理与实例.生物上程进展,2001,(4)：57-62.
192.陈捷胤,戴小枫.棉花对黄萎病的抗病机制研究进展川.分子植物育种,2005,3(3)：427-435.
193.陈旭生,陈永萱.棉花黄萎病菌鉴定技术进展.棉花学报,1997,9(2)：64-67.
194.董志强,何钟佩,翟学军.缩节安处理对棉花主茎生长过程及其激素水平的影响.湖北农学报,1999,14(增刊)：60-63.
195.冯国艺,姚炎帝,杜明伟,田景山,罗宏海,张亚黎,张旺锋.缩节安对干旱区杂交棉冠层结构及群体光合生产的调节.棉花学报,2012,24(1)：44-51.
196.付慧娟,刘方,黎绍惠等.半野生棉抗黄萎病筛选及根系分泌物对黄萎病菌的影响.棉花学报,2011,23(5)：387-393.
197.甘莉,吕金殿.棉花品种中糖及单宁与抗黄萎病的关系.陕西农业科学,1989,(6)：13-14.
198.郭宾会,高双成,景蕊莲等.小麦苗期水分胁迫诱导差异表达cDNA的研究.生物技术通报,2003(2)：6-11.
199.何钟佩,闵祥佳,李丕明等.植物生长延缓剂DPC对棉铃内源激素水平和棉铃发育影响的研究.作物学报,1990,16(3)：252-257.
200.何钟佩,宋绍省.植物生长延缓荆DPC对棉叶生理功能的调节作用.北京农业大学学报,1991,17(增刊)：21-25.顾本康,马存.中国棉花抗病育种.南京：江苏科学技术出版社,1996,73.
201.郝中娜,陶荣祥.WRKY转录因子超家族的研究.生物科学,2006,18(2)：175-179.
202.蒋明义,郭绍川.水分亏缺诱导的氧化胁迫和植物的抗氧化作用.植物生理学通讯,1996,32(2)：144-150.
203.康宗利,杨玉红,张立军.植物响应干旱胁迫的分子机制.玉米科学,2006,14(2)：96-100.
204.梁玉芹,刘云,刘文,宋炳彦.缩节安对樱桃番茄植株生长、产量及品质的影响.河北农业科学,2008,12(10)：23-24.
205.李丕明,何钟佩,李召虎.棉花应用缩节安(DPC)化控技术研究概况与进展.作物杂志,1991(2)：1-3.
206.刘春芳,王志利,罗湘宁.缩节安(DPC)在棉花生产上的应用研究.西北农业学报,1997,5(1)：81-83.
207.刘华玲,马欣荣.植物抗旱分子机理研究进展.世界科技研究与发展,2006,28(6)：33-40.
208.路运才,石云素,宋燕春等.玉米苗期水分胁迫下相关基因差异表达研究.玉米科学,2006(3)：278-282.
209.马存,简桂良,孙文姬.我国棉花抗黄萎病育种现状、问题及对策.中国农业科学,1997,30(2)：58-64.
210.马宗斌,房卫平,谢德意.氮肥和DPC用量对棉花叶片叶绿素含量和SPAD值的影响.棉花学报,2009,21(3)：224-229.
211.沈其益.棉花病害-基础理论与防治.北京,科学出版社,1992,128-153.
212.宋雯雯,李文滨,王继安等.干早胁迫下大豆幼苗根系基因的表达谱分析.中国农业科学,2010,43(22)：4579-4586.
213.石磊岩,简桂良,孙文姬.我国棉花黄萎病研究现状与方向.植物保护学报,1998,25(2)：103-107.
214.时忠杰,胡哲森,李荣生.水分胁迫与活性氧代谢.贵州大学学报(农业与生物科学版),2002,21(2)：140-145.
215.邵莉相,孟小雄.植物生长调节剂应用手册.北京：金盾出版社,2004.
216.田秀明,杜利锋.棉花对枯、黄萎病的抗性与过氧化物酶活性的关系阴.植物病理学报,1991,21(1)：94-97.
217.杨青华,唐予迪,李伶俐.DPC系统化控对麦套春棉生理特性的影响.河南农业大学学报,1996,30(2)：164-167.
218.王宏,张雄伟.植物生长调节剂在棉花上的应用及前景.中国棉花,1994,21(3)：7-9.
219.王娟,李德全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢.植物学通报,2001,18(4)：459-465.
220.王晓光,马远征,王晓辉,李玲,罗云波,朱本忠.破色期番茄果实均一化cDNA沉默文库的构建和功能基因筛选模型的初步建立.生物化学与生物物理进展,2008,8：1079-1083.
221.徐幼平,徐秋芳,宋晓毅.病毒诱导的基因沉默.浙江大学学报,2008,34(2)：119-131.
222.姚焕章.棉花抗黄萎病性能的初步研究.华东农业科学通报,1956,(7)：354.
223.郑明刚,孙修勤,张进兴.皱纹盘鲍肝和肾cDNA文库的构建及免疫相关基因的初步分析.高技术通讯,2007,3：319-324.
224.周运刚,王傻刚,马天文.不同DPC(缩节安)处理对棉花生理生化特性的影响.新疆农业科学,2010,47(6)：1142-1146.
2. Adams S S, Rouse D I, Bowden R L. Performance of alternative versions of POTWIL:a computer model that simulates the seasonal growth of Verticillium infected potato. Am Potato J.,1987,64: 429-435.
3. Ait A T, Swain S M. The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. Plant J.,1997,11:443-454.
4. Anderson J V, Chevone B I, Hess J L. Seasonal variation in the antioxidant systerm of eastern white pine needles. Plant Phy,1992,98:501-508.
5. Andrew L P. Gibberellins in Arabidopsis. Plant Physiol Biochem.,1998,36:115-124.
6. Anjum F, Yaseen M, Rasul E, et al. Water stress in barley (Hordeum vulgare L.). I. Effect on chemical composition and chlorophyll contents. Pakistan J. Agr. Sci.2003,40:45-49.
7. Anna C M, Sylvain M, Alain V, et al. Arabidopsis OST1 Protein Kinase Mediates the Regulation of Stomatal Aperture by Abscisic Acid and Acts Upstream of Reactive Oxygen Species Production. Plant Cell,2002,14(12):3089-3099.
8. Annette B and Matthias L. VIGS-genomic goes functional. Trends in Plant Sci,2009,15(1):1-4.
9. Asai T, Tena G, Plotnikova J, et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature,2002.415:977-983.
10. Au K F, Jiang H, Lin L, et al. Detection of splice junctions from paired-end RNA-seq data by SpliceMap. Nucleic Acids Res,2010,38(14):4570-4578.
11. Ausubel F M. Are innate immune signaling pathways in plants and animals conserved? Nat Immunol,2005,6:973-979.
12. Baulcombe D C. Fast forward genetics based on virus-induced gene silencing. Curr Opin in Plant Biol,1999,2:109-113.
13. Baulcombe D C. RNA silencing in plants. Nature,2004,431 (7006):356-363.
14. Becker A, Lange M. VIGS-genomics goes functional. Trends Plant Sci,2010,15:1-4.
15. Bensen R J, Johal G S. Clonging and characterization of the maize Anl gene. Plant Cell,1995,7: 75-84.
16. Bergmann D C, Lukowitz W, Somerville C R. Stomatal development and pattern controlled by a MAPKK kinase. Science,2004,304:1494-1497.
17. Boller T, Felix G A renaissance of elicitors:perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol,2009,60:379-406.
18. Bota J, Flexas J, Medrano H. Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? New Phytol,2004,162:671-681.
19. Boudsocq M, Barbier-Brygoo H, Lauriere C. Identification of nine sucrose nonfermenting related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem,2004,279(40):41758-41766.
20. Burton R A, Gibeaut D M, Bacie A, et al. Virus-induced silencing of a plant cellulose synthase gene. Plant cell,2000,12:691-705.
21. Cathey H M. Physiology of growth retarding chemicals. Annu Rev Plant Physiol.,1964,15: 271-302.
22. Cogoni C, Macino G. Post-transcriptional gene silencing across kingdoms. Curr Opin Genet Dev., 2000,10(6):638-643.
23. Colcombet J, Boisson-Dernier A, Ros-Palau R, et al. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 are essential for tapetum development and microspore maturation. Plant Cell,2005,17:3350-3361.
24. Cornic G, Massacci A. Leaf photosynthesis under drought stress, in:Baker N.R., (Ed.), Photosynthesis and the Environment, Kluwer Academic Publishers, The Netherlands.1996.
25. Chen L, Zhang L, Yu D. Wounding-induced WRKY8 is involved in basal defense in Arabidopsis. Mol Plant-Microbe Interact,2010,23:558-565.
26. Chen Z J, Scheffler B E, Dennis E, et al. Toward sequencing cotton(Gossypium) genomes. Plant Physiol.,2007,145:1303-1310.
27. Chinchilla D, Shan L, He P, et al. One for all:the receptor-associated kinase BAK1. Trends Plant Sci.,2009.14:535-541.
28. Chinchilla D, Zipfel C, Robatzek S, et al. Flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature,2007,448:497-500.
29. Cho K Y, Sakurai A, Kamiya Y, et al. Effects of the new plant growth retardants of quaternary ammonium iodides on gibberellin biosynthesis in Gibberella fujikuroi. Plant Cell Physiol.,1979, 20:75-81.
30. Choi H I, Park H J, Park J H, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol.,2005,139:1750-1761.
31. Cutler S R, Rodriguez P L, Finkelstein R R, et al. Abscisic acid:Emergence of a core signaling network. Annu Rev Plant Biol.,2010,61:651-679.
32. Dawson W O. Gene silencing and virus resistance:a common mechanism. Trends Plant Sci.,1996, 1:107-108.
33. DeLucia E H, Heckathorn S A. The effect of soil drought on water-use efficiency in a contrasting Great Basin desert and Sierran montane species. Plant Cell Environ,1989,12:935-940.
34. Dever J, Wheeler T, Kelly C. Registration of CA 4002 cotton germplasm line partially resistant to Verticillium wilt. Journal of Plant Regist.,2013,7:1-7.
35. Dicks J W. Mode of action of growth retardants. See Ref.25a,1980, pp:1-14.
36. Dinesh-Kumar S P, Anandalakshmi R., Marathe R, et al. Virus-induced gene silencing. Methods Mol Biol.,2003,236:287-294.
37. Ding X S, Rao C S, Nelson RS. Analysis of gene function in rice through virus-induced gene silencing. Methods Mol Biol.,2007,354:145-160.
38. Ding X S, Schneider W L, Chaluvadi S R, et al. Characterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts. Mol Plant Microbe Interact., 2006,19:1229-1239.
39. Dong J, Chen C, Chen Z. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol.,2003,51:21-37.
40. Edgren H, Murumagi A, Kangaspeska S, et al. Identification of fusion genes in breast cancer by paired-end RNA-sequencing. Genome Biol.,2011,12(1):R6.
41. Egilla J N, Davies Jr F T, Boutton T W. Drought stress influences leaf water content, photosynthesis, and water-use efficiency of Hibiscus rosa-sinensis at three potassium concentrations. Photosynthetica.,2005,43:135-140.
42. Eulgem T, Rushton P J, Robatzek S, et al. The WRKY superfamily of plant transcription factors. Trends Plant Sci.,2000,5:199-206.
43. Eynck C, Koopmann B, Grunewald-Stoecker G, et al. Differential interactions of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques. Eur J Plant Pathol.,2007,118(3):259-274.
44. Fahnenstich H, Scarpeci T E, Valle E M, et al. Generation of hydrogen peroxide in chloroplasts of Arabidopsis overexpressing glycolate oxidase as an inducible system to study oxidative stress. Plant Physiol.,2008,148:719-729.
45. Farooq M, Wahid A, Kobayashhi N, et al. Plant drought stress:effects, mechanisms and management. Agronomy,2009,29:185-212.
46. Filichkin S A, Priest H D, Givan S A, et al. Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Res.,2010,20(1):45.
47. Finkelstein R, Gampala S S, Lynch T J, et al. Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3. Plant Mol. Biol.,2005,59:253-267.
48. Fradin E F, Thomma B P. Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol Plant Pathol.,2006,7:71-86.
49. Fradin E F, Zhang Z, Juarez Ayala J C, et al. Genetic dissection of Verticillium wilt resistance mediated by tomato Vel. Plant Physiol.,2009,150:320-332.
50. Fu D Q, Zhu B Z, Zhu H L, et al. Eahancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol. Cell,2006,1:153-160.
51. Fu D Q, Zhu B Z, Zhu H L, et al. Vims-induced gene silencing in tomato fruit. Plant J.,2005,43: 99-308.
52. Fu J, Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot.,2001,45:105-114.
53. Fujii H, Verslues P E, Zhu J K. Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell,2007, 19(2):485-494.
54. Fujii H, Verslues P E, Zhu J K. Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo. Proc. Natl. Acad. Sci. USA,2011,108(4):1717-1722.
55. Fujii H, Zhu J K. Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. Proc. Natl. Acad. Sci. USA,2009,106(20): 8380-8385.
56. Fujii H, Zhu J K. Osmotic stress signaling via protein kinases. Cell Mol. Life Sci.,2012, 69(19):3165-3173.
57. Fujita Y, Nakashima K, Yoshida T, et al. Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol., 2009,50:2123-2132.
58. Gao M, Wang X, Wang D, et al. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Micro.,2009,6:34-44.
59. Gao X, Britt R C, Shan L, et al. Agrobacterium-mediated virus-induced gene silencing assay in cotton. J Vis Exp.,2011a.
60. Gao X, Shan L. Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing. Methods Mol. Biol.,2013,975:157-165.
61. Gao X, Wheeler T, Li Z, et al. Silencing GhNDRl and GhMKK2 compromises cotton resistance to Verticillium wilt. Plant J.,2011b,66:293-305.
62. Garrod J F, Hewitt H G, Copping L G, et al. A new group of ternary sulphonium growth retardants. See Ref.25a,1980, pp:67-74.
63. Gossmann K. Plant growth retardants:their mode of action and benefit for physiological research. See Ref.93a,1992, pp:788-797.
64. Gou X, Yin H, He K, et al. Genetic evidence for an indispensable role of somatic embryogenesis receptor kinases in brassinosteroid signaling. PLoS Genet.,2012,1002452.
65. Hamilton A J, Baulcombe D C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science,1999,286:950-952.
66. Hamilton A J, Bouzayen M, Grierson D. Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast. Proc. Natl. Acad. Sci. U S,1991,88(16):7434-7437.
67. Hashmi J A, Zafar Y, Arshad M, et al. Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes,2011,42: 286-296.
68. Hayward A, Padmanabhan M, Dinesh-Kumar S P. Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species. Plant Reverse Genetics:Methods and Protocols, Methods in Molecular Biology,2011,678:55-63.
69. He K, Gou X, Yuan T, et al. BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr. Biol.,2007,17:1109-1115.
70. Hecht V, Vielle-Calzada J P, Hartog M V, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol.,2001,127:803-816.
71. Hedden P. The action of plant growth retardants at the biochemical level. See Ref.132a,1990, pp: 322-332.
72. Heese A, Hann D R, Gimenez-Ibanez S, et al. The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc. Natl. Acad. Sci. USA,2007,104:12217-12222.
73. Hinchliffe D J, Meredith W R, Yeater K M, et al. Near-isogenic cotton germplasm lines that differ in fiberbundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling. Theor.Appl. Genet,2010,120:1347-1366.
74. Hoen P A, Ariyurek Y, Thygesen H H, et al. Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray plantform. Nucleic Acids Research,2008,36(21):e141.
75. Hoyos M E, Zhang S. Calcium-independent activation of salicylic acid-induced protein kinase and 40-kDa protein kinase by hyperosmotic stress. Plant Physiol.,2000,122:1355-1363.
76. Huisman O C. Interrelations of Root Growth Dynamics to Epidemiology of Root-Invading Fungi. Annu. Review of Phytopathol.,1982,20:303-327.
77. Idris A M, Tuttle J R, Roberson D, et al. Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physio and Mol Plant Pathol.,2010,75:13-22.
78. Joo J H, Wang S, Chen J, el al. Different signallingand cell death roles of heterotrimeric G protein a and b subunits in the Arabidopsis oxidativestress response to ozone. Plant Cell,2005,17: 957-970.
79. Jostr, Aitschmied L, Bloem E. Expression profiling of metabolic gene in response to methyl jasm on atereveais regulation of genes of primary and secondary sulfur related pathways in Arabidopsis thalinan. Photosynth Research,2005,86:491-508.
80. Jung J. Possibilities for optimization of plant nutrition by new agrochemical substances-especially in cereals. In Plant Regulation and Word Agriculture, ed. TK Scott,1979, pp.279-307. New York: Plenum.
81. Kalde M, Barth M, Somssich I E, et al. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signalling pathways. Mol Plant Microbe Interact,2003, 16:295-305.
82. Kaloshian I. Virus-Induced Gene silencing in plants roots. Plant-Pathogen Interactions:Methods and Protocols. Methods in Molecular Biology,2007,354:173-181.
83. Karlova R, Boeren S, Russinova E, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex includes BRASSINOSTEROID-INSENSITIVE1. Plant Cell,2006,18:626-638.
84. Kawchuk L M, Hachey J, Lynch D R, et al. Tomato Ve disease resistance genes encode cell surface-like receptors. Proc. Natl. Acad. Sci. U S A.,2001,98:6511-6515.
85. Kim K C, Lai Z, Fan B, et al. Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense. Plant Cell,2008,20:2357-2371.
86. Kinoshita T, Cano-Delgado A, Seto H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature,2005,433:167-171.
87. Klingler J P, Batelli G, Zhu J K. ABA receptors:The START of a new paradigm in phytohormone signalling. J Exp Bot.,2010,61(12):3199-3210.
88. Kishor P B K, Hong Z, Miao G H, el al. Over expression of A-pyrroline-5-carboxylate synthetase increases proline products and confers osmo-tolerance in transgenic plants. Plant Physio.,1995, 108:1387-1394.
89. Knoth C, Ringler J, Dangl J L, el al. Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica. Mol Plant Microbe Interact,2007,20:120-128.
90. Koornneef M, and van der Veen J H. Induction and analysis of gibberellin-sensitive mutants in Arabidopsis thaliana (L.) Heynh. Theor. Appl. Genet.,1980,58:257-263.
91. Koornneef M, van Eden J, Hanhart C J, et al. Genetic fine-structure of the GA-7 locus in the higher plant Arabidopsis thaliana (L.) Heynh. Genet. Res. Camb.,1983,41:57-68.
92. Kumagai M H, Donson J, Della-Cioppa G, et al. Cytoplamic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc. Natl. Acad. Sci. USA,1995,92(5):1679-1683.
93. Lacape J M, Claverie M, Vidal R O, et al. Deep sequencing reveals differences in the transcriptional landscapes of fibers from two cultivated species of cotton. PLoS ONE,2012,7(11): e48855. Doi:10.1371/journal. pone.0048855.
94. Lacomme C, Hrubikova K, Hein I. Enhancement of virus-induced gene silencing through viral based production of inverted repeats. Plant J.,2003,34(4):543-553.
95. Lai Z, Li Y, Wang F, et al. Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense. Plant Cell,201 la,23:3824-3841.
96. Lai Z, Wang F, Zheng Z, et al. A critical role of autophagy in plant resistance to necrotrophic fungal pathogens. Plant J.,201lb,66:953-968.
97. Lazaridou M, Koutroubas S D. Drought effect on water use efficiency of berseem clover at various growth stages. New directions for a diverse planet:Proceedings of the 4th International CropScience Congress Brisbane, Australia,2004,26 Sept-1 Oct 2004.
98. Li J, Brader G, Kariola T, et al. WRKY70 modulates the selection of signaling pathways in plant defense. Plant J.,2006,46:477-491.
99. Li J, Brader G, Palva E T. The WRKY70 transcription factor:a node of convergence for jasmonate-mediated and salicylatemediated signals in plant defense. Plant Cell.,2004,16:319-331.
100. Li Z, An L H, Fu Q, et al. Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper. Ecotoxicology,2012,21:260-267.
101. Linser H, Bettner L. Wachstumsretardantien. J. plant Nutr. Soil Sci.,1972,132:105-143.
102. Lindbo J A, Silva-Rosales L, Proebsting W M, et al. Induction of a Highly Specific Antiviral State in Transgenic Plants:Implications for Regulation of Gene Expression and Virus Resistance. Plant cell,1993,5(12):1749-1759.
103. Liu Y, Schiff M, Dinesh-Kumar S P. Involvement of MEK1MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, Col 1 and CRT1 in N-mediated resistance to tobacco mosaic virus. Plant J., 2004,38:800-809.
104. Lu T T, Lu G J, Fan D L, et al. Function an notation of the rice transcriptome at single-nucleotide resolution by RNA-seq. Genome Res.,2010,20(9):1238-1249.
105. Maher C A, Palanisamy N, Brenner J C, et al. Chimeric transcript discovery by paired-end transcriptome sequencing. Proc. Natl. Acad. Sci. USA,2009,106(30):12353-12358.
106. Maqbool A, Abbas W, Rao A Q, et al. Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum. Biotechnol Progr.,2010,26:21-5.
107. Mei C S, Zhou X, Yang Y. Use of RNA interference to dissect defense-signaling pathways in rice. Plant-Pathogen Interactions:Methods and Protocols. Methods in Molecular Biology,2007, vol. 354:161-172.
108. Mitchell J W, Wirwille J W, Weil L. Plant growth-regulating properties of some nicotinium compounds. Science,1949,110:252-254.
109. Monakhova O F, Chernyadev I.I. Protective role of kartolin-4 in wheat plants exposed to soil drought, Appl. Biochem. Micro.,2002,3:373-380.
110. Mortazavi A, Williams B A, McCue K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat. Methods,2008,5(7):621.
111. Muangsan N, Robertson D. Geminivirus vectors for transient gene silencing in plant. Methods Mol. Biol.,2004,265:101-115.
112. Nagalakshmi U, Wang Z, Waem K, et al. The transcriptional landscape of the yeast genome defined by RNA sequencing. Science,2008,320(5881):1344-1349.
113. Nakashima K, et al. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK21/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol.,2009,50(7):1345-1363.
114. Nerd A, Nobel P S. Effects of drought on water relations and nonstructural carbohydrates in cladodes of Opuntia ficus-indica, Physiol. Plant,1991,81:495-500.
115. Nishikura K. A short primer on RNAi:RNA-directed RNA polymerase acts as a key catalyst. Cell, 2001,107(4):415-418.
116. Okoniewski M J, Miller C J. Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations. BMC Bioinformatics,2006,7(1):276. Cell,2001,107(4):415-418.
116. Okoniewski M J, Miller C J. Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations. BMC Bioinformatics,2006,7(1):276.
117. Ozsolak F, Milos PM. RNA sequencing:advances, challenges and opportunities. Nat. Rev. Genet.,2011,12(2):87-98.
118. Pang M X, Percy R G, Stewart J M, et al, Comparative transcriptome analysis of Pima and Acala cotton during boll development using 454 pyrosequencing technology. Molecular Breeding,2012, 30(2):1143-1152.
119. Peuke A D, Hartung W, Schraml C, et al. Identification of drought sensitive beech ecotypes by physiological parameters, New Phytol.,2002,154:373-388.
120. Pflieger S, Blanchet S, Camborde L, et al. Efficient virus-induced gene silencing in Arabidopsis using a'one-step'TYMV-derived vector. Plant J.,2008,56:678-690.
121. Qiu J L, Zhou L, Yun B W, et al. Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1. Plant Physiol.,2008,148:212-222.
122. Qu J, Ye J, Geng Y F, et al. Dissecting functions of KATANIN and WRINKLED 1 in cotton fiber development by virus-induced gene silencing. Plant Physiol.,2012,160:738-748.
123. Rademacher W. Biochemical effects of plant growth retardants. See Ref.49a,1991, pp.169-200
124. Rao A Q, Bakhsh A, Nasir I A, et al. Phytochrome B mRNA expression enhances biomass yield and physiology of cotton plants. Afr. J. Biotechnol.,2011,10:1818-26.
125. Ratcliff F, Martin-Hernandez A M, Baulcombe D C. Tobacco rattle virus as a vector for analysis of gene function by silencing. The Plant journal,2001,25(2):237-245.
126. Ratcliff F, MacFarlane S, Baulcombe D C. Gene silencing without DNA:RNA-mediated cross protection between viruses. Plant Cell,1999,11:1207-1215.
127. Ravi V M, Yao Liu, Tracy R R, et al. The tomato Fni3 Lysine-63-specific ubiquitin-conjugating enzyme and Suv ubiquitin E2 Variant positively regulate plant immunity. Plan Cell,2013,25: 3615-3631.
128. Ren B Z. Biochemistry and Clinical medicine. Chmlgsha, Hunan Sience and Technical Press,1993.
129. Richman A S, Gijzen M. Diterpene synthesisin Stevia rebaudiana:recruitment and upregulation of key enzymes from the gibberellin biosynthetic pathway. Plant J.,1999,19:411-421.
130. Roux M, Schwessinger B, Albrecht C, et al. The Arabidopsis leucine-rich repeat receptor-like kinases BAK.1/SERK3 and BKK1/SERK4 are required for innate immunity to hemibiotrophic and biotrophic pathogens. Plant Cell,2011,23:2440-2455.
131. Royce T E, Rozowsky J S, Gerstein M B. Toward a universal microarray:prediction of gene expression through nearest-neighbor probe sequence identification. Nucleic Acids Res.,2007, 35(15).
132. Ruiz M T, Voinnet, Baulcombe D C. Initiation and maintenance of virus-induced gene silencing. Plant cell,1998,10(6):937-946.
133. Samuel M A, Miles G P, Ellis B. Ozone treatment rapidly activates MAP kinase signalling in plants. Plant J.,2000,22:367-376.
134. Sauter H. Chemical aspects of some bioregulators. In Bioregulators:Chemistry and Uses, ed. RL Ory, FR Rittig,1984, pp.9-21. Washington, DC:Am. Chem. Soc.
135. Scofield S R, Huang L, Brandt A S, et al. Development of a virus induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol.,2005,138:2165-2173.
136. Schmidt E D, Guzzo F, Toonen M A, et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development,1997,124:2049-2062.
137. Schulze B, Mentzel T, Jehle A K, et al. Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1. J. Biol. Chem., 2010,285:9444-9451.
138. Shan L, He P, Li J, et al. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Micro., 2008,4:17-27.
139. Shan L, He P, Sheen J. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host Microb.,2007,1:167-174.
140. Sharkey T D. Water stress effects on photosynthesis. Photosynthetica,1990,24,651-661.
141. Shendue J. The beginning of the end for microarrays? Nature Methods,2008,5(7):585-587.
142. Shiu S H, Bleecker A B. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc. Natl. Acad. Sci. USA,2001,98:10763-10768.
143. Shiu S H, Bleecker A B. Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis. Plant Physiol.,2003,132:530-543.
144. Smith M S, Yamaguchi S, Ait-Ali, et al. The first step of gibberellin biosynthesis in pumpkin is catalyzed by at least two copalyl diphosphate synthases encoded by differentially regulated genes. Plant Physiol.,1998,118:1411-1419.
145. Smith T M, Anderson J C, Martin G B, et al. Applications and advantages of virus-induced gene silencing for gene function studies in plant. Plant J,2004,39(5):734-746.
146. Smith T M, Schiff M, Liu Y, et al. Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol,2006,142(1):21-7.
147. Sultan M, Schulz M H, Richard H, et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science,2008,321(5891):956.
148. Sun T-p, Goodman H M, Ausubel F M. Cloning the Arabidopsis GA1 locus by genomic subtraction. Plant Cell,1992,4:119-128.
149. Sunilkumar G, Campbell L M, Puckhaber L, et al. Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc. Natl. Acad. Sci. USA,2006,103: 18054-18059.
150. Takeshi N, Jung-Bum L. cDNA clonging, functional expression and characterization of ent-Copalyl Diphosphate Synthases from Scoparia dulcis L. Plant Science,2005,169:760-767.
151. Tallys P W. Association of tylosis and hyperplasia of the xylem with vascular invasion of the hop by Verticillium alboatrum. Transactions British Mycological Society TALBOYS,1958,41: 249-260.
152. Telma E S, Man'a I Z, Bernd M R, et al. Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana. Plant Mol. Biol.,2013,83:265-277.
153. Tezara W, Mitchell V J, Driscoll S D, et al. Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. Nature,1999,401:914-917.
154. Thomas C L, Jones L, Baulcombe D C, et al. Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. Plant J.,2001,25(4):417-425. substance. I. Chemical structure and bioassay. J. Biol. Chem.,1960a,235:475-479.
157. Tolbert N E. (2-Chloroethyl)-trimethylammoniumchloride and related compounds as plant growth substance. II. Effect on growth of wheat. Plant Physiol.,1960b,35:380-385.
158. Tomonobu T, Takuma K. Clonging and characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat:Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes. Biosci. Biotechnol. Biochem.,2009,73(3):772-775.
159. Travella S, Klimm T E, Keller B. RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat. Plant Physiol,2006.142(1):6-20.
160. Tuttle J R, Idris A M, Brown J K, et al. Geminivirus mediated gene silencing from Cotton leaf crumple virus is enhanced by low temperature in cotton. Plant Physiol.,2008,148:41-50.
161. Van Kammen A. Virus-induced gene silencing in infected and transgenic plants. Trends plant Sci., 1997,2:409.
162. Voinnet O. RNA silencing as a plant immune system against viruses. Trends Genet.,2001,17:449.
163. Vu J C V, Gesch R W, Allen L H, et al. CO2 enrichment delays a rapid, drought-induced decrease in Rubisco small subunit transcript abundance, J. Plant Physiol.,1999,155:139-142.
164. Wahid A, Rasul E. Photosynthesis in leaf, stem, flower and fruit, in:Pessarakli M. (Ed.), Handbook of Photosynthesis,2nd ed., CRC Press, Florida,2005, pp.479-497.
165. Wang G D, Ellendorff U, Kemp B, et al. Genome-wide functional investigation into the roles of receptor-like proteins in Arabidopsis. Plant physiol.,2008,147:503-517.
166. Wang K, Wang Z, Li F, et al. The draft genome of a diploid cotton Gossypium raimondii. Nat Genet.,2012,44:1098-1103.
167. Wang P, Liang X, Giorgia B, et al. Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action. PNAS,2013,110:11205-11210.
168. Waterhouse P M, Helliwell C A. Exploring plant genomes by RNA-induced gene silencing. Nature Rev Genet.,2003,4(1):29-38.
169. Wendel J F. Genome evolution in polyploids. Plant Mol Biol.,2000,42:225-249.
170. Wesley S V, Helliwell C A, Smith N A, et al. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J.,2001,27(6):581-590.
171. Wettstein D V, Gough S, Kannangara C G. Chlorophyll biosynthesis. Plant Cell,1995,7: 1039-1057.
172. Wilhelm B T, Marguerat S, Watt S, et al. Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution. Nature,2008,453(7199):1239-1243.
173. Wilhelm R. Growth retardants:Effects on Gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol. Plant Mol. Biol.,2000,51:501-531.
174. Xiang L X, He D, Dong W R, et al. Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marinefish. BMC Genomaics,2010,11(1):472.
175. Xu M, Ross W. Functional characterization of the rice kaurene synthase-like gene family. Phytochem.,2007,68:312-326.
176. Yamaguchi S, Sun T-p, Kawaide H, et al. The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant Physiol.,1998b,116:1271-1278.
177. Ye B, Gressel J. Transient oxidant-induced antioxigant transcript and enzyme levels correlate with greater oxidant-resistance in paraquant-resistant Conyza bonariensis. Planta,2000,211:50-61.
178. Yokota A, Kawasaki S, Iwano M, et al. Citrulline and DRIP-1 Protein (ArgE Homologue) in Drought Tolerance of Wild Watermelon. Ann. Bot.,2002,89:825-832.
179. Yoshida T, Fujita Y, Sayama H, et al. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J.,2010,61:672-685.
180. Yu D, Chen C, Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell,2001,13:1527-1540.
181. Yu J, Kohel R J, Smith C W. The construction of a tetraploid cotton genome wide comprehensive reference map. Genomics.,2010,95:230-240.
182. Yue Y, Zhang M, Zhang J, et al. Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton. J Exp Bot.,2012,63(10):3741-3748.
183. Zeeh B, Konig K H, Jung J. Development of a new plant growth regulator with biolodical activity related to CCC. Kemia(Helsinki),1974,9:621-623.
184. Zhang G J, Guo G W, Hu X D, et al. Deep RNA sequencing at single base-pair resolution reveals high complexity of the rice transcriptome. Genome Res.,2010,20(5):646-654.
185. Zhang H, Shen G X, Kuppu S, et al. Creating drought-and salt-tolerant cotton by overexpressing a vacuolar pyrophosphatase gene. Plant Signaling&Behavior,2011,6 (6):861-863.
186. Zhang H B, Li Y, Wang B, and Chee P W. Recent advances in cotton genomics. Int. J. Plant Genomics,2008,742304.
187. Zheng Z, Qamar S A, Chen Z, et al. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J.,2006,48:592-605.
188. Zhong W, Mark G, Michael S. Applications of next-generation sequencing. Nature Reviews Genetics,2009,10:57-63.
189. Zhu J K. Salt and drought stress signal transduction in plants. Ann. Rev. Plant Bio.,2002,53: 247-273.
190.白克智.植物生长调节剂实用问答.北京：化学工业出版社,1998,16.
191.陈峰,李洁.酵母单杂交的原理与实例.生物上程进展,2001,(4)：57-62.
192.陈捷胤,戴小枫.棉花对黄萎病的抗病机制研究进展川.分子植物育种,2005,3(3)：427-435.
193.陈旭生,陈永萱.棉花黄萎病菌鉴定技术进展.棉花学报,1997,9(2)：64-67.
194.董志强,何钟佩,翟学军.缩节安处理对棉花主茎生长过程及其激素水平的影响.湖北农学报,1999,14(增刊)：60-63.
195.冯国艺,姚炎帝,杜明伟,田景山,罗宏海,张亚黎,张旺锋.缩节安对干旱区杂交棉冠层结构及群体光合生产的调节.棉花学报,2012,24(1)：44-51.
196.付慧娟,刘方,黎绍惠等.半野生棉抗黄萎病筛选及根系分泌物对黄萎病菌的影响.棉花学报,2011,23(5)：387-393.
197.甘莉,吕金殿.棉花品种中糖及单宁与抗黄萎病的关系.陕西农业科学,1989,(6)：13-14.
198.郭宾会,高双成,景蕊莲等.小麦苗期水分胁迫诱导差异表达cDNA的研究.生物技术通报,2003(2)：6-11.
199.何钟佩,闵祥佳,李丕明等.植物生长延缓剂DPC对棉铃内源激素水平和棉铃发育影响的研究.作物学报,1990,16(3)：252-257.
200.何钟佩,宋绍省.植物生长延缓荆DPC对棉叶生理功能的调节作用.北京农业大学学报,1991,17(增刊)：21-25.顾本康,马存.中国棉花抗病育种.南京：江苏科学技术出版社,1996,73.
201.郝中娜,陶荣祥.WRKY转录因子超家族的研究.生物科学,2006,18(2)：175-179.
202.蒋明义,郭绍川.水分亏缺诱导的氧化胁迫和植物的抗氧化作用.植物生理学通讯,1996,32(2)：144-150.
203.康宗利,杨玉红,张立军.植物响应干旱胁迫的分子机制.玉米科学,2006,14(2)：96-100.
204.梁玉芹,刘云,刘文,宋炳彦.缩节安对樱桃番茄植株生长、产量及品质的影响.河北农业科学,2008,12(10)：23-24.
205.李丕明,何钟佩,李召虎.棉花应用缩节安(DPC)化控技术研究概况与进展.作物杂志,1991(2)：1-3.
206.刘春芳,王志利,罗湘宁.缩节安(DPC)在棉花生产上的应用研究.西北农业学报,1997,5(1)：81-83.
207.刘华玲,马欣荣.植物抗旱分子机理研究进展.世界科技研究与发展,2006,28(6)：33-40.
208.路运才,石云素,宋燕春等.玉米苗期水分胁迫下相关基因差异表达研究.玉米科学,2006(3)：278-282.
209.马存,简桂良,孙文姬.我国棉花抗黄萎病育种现状、问题及对策.中国农业科学,1997,30(2)：58-64.
210.马宗斌,房卫平,谢德意.氮肥和DPC用量对棉花叶片叶绿素含量和SPAD值的影响.棉花学报,2009,21(3)：224-229.
211.沈其益.棉花病害-基础理论与防治.北京,科学出版社,1992,128-153.
212.宋雯雯,李文滨,王继安等.干早胁迫下大豆幼苗根系基因的表达谱分析.中国农业科学,2010,43(22)：4579-4586.
213.石磊岩,简桂良,孙文姬.我国棉花黄萎病研究现状与方向.植物保护学报,1998,25(2)：103-107.
214.时忠杰,胡哲森,李荣生.水分胁迫与活性氧代谢.贵州大学学报(农业与生物科学版),2002,21(2)：140-145.
215.邵莉相,孟小雄.植物生长调节剂应用手册.北京：金盾出版社,2004.
216.田秀明,杜利锋.棉花对枯、黄萎病的抗性与过氧化物酶活性的关系阴.植物病理学报,1991,21(1)：94-97.
217.杨青华,唐予迪,李伶俐.DPC系统化控对麦套春棉生理特性的影响.河南农业大学学报,1996,30(2)：164-167.
218.王宏,张雄伟.植物生长调节剂在棉花上的应用及前景.中国棉花,1994,21(3)：7-9.
219.王娟,李德全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢.植物学通报,2001,18(4)：459-465.
220.王晓光,马远征,王晓辉,李玲,罗云波,朱本忠.破色期番茄果实均一化cDNA沉默文库的构建和功能基因筛选模型的初步建立.生物化学与生物物理进展,2008,8：1079-1083.
221.徐幼平,徐秋芳,宋晓毅.病毒诱导的基因沉默.浙江大学学报,2008,34(2)：119-131.
222.姚焕章.棉花抗黄萎病性能的初步研究.华东农业科学通报,1956,(7)：354.
223.郑明刚,孙修勤,张进兴.皱纹盘鲍肝和肾cDNA文库的构建及免疫相关基因的初步分析.高技术通讯,2007,3：319-324.
224.周运刚,王傻刚,马天文.不同DPC(缩节安)处理对棉花生理生化特性的影响.新疆农业科学,2010,47(6)：1142-1146.