鲤鱼白细胞介素-1β基因克隆、鉴定及时空转录分析
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摘要
本研究应用核酸杂交法,以鲤鱼白细胞介素-1βcDNA部分片段为探针,筛选丝裂原刺激的鲤鱼外周血白细胞cDNA文库,克隆了白细胞介素-1β全长cDNA序列,测得全长序列为1213bp,包含831bp的开放阅读框,推测编码276个氨基酸：根据所获得的全长cDNA序列设计引物,以鲤鱼脾脏基因组DNA为模板,克隆了白细胞介素-1β基因组DNA,序列全长为2451bp,其中含有6个内含子和7个外显子,并且外显子和内含子剪接位点都符合GT-AG规则；荧光定量PCR分析鲤鱼正常白细胞和有丝分裂原刺激的白细胞中,白细胞介素-1β基因不同时间段以及不同刺激物作用后的转录差异,实验结果表明：有丝分裂原刺激的白细胞中白细胞介素-1β转录增强,特别是LPS、PHA联合刺激后转录增强极显著,但是随着时间的延长转录增强的幅度呈下降趋势,可见白细胞介素-1β的转录对不同的诱导物敏感度不同,推测白细胞受刺激早期发挥免疫作用。
At present,the higher animals have been on the role of cytokines and their insight into the mechanism, and some cytokines can be used in clinical treatment.such as interferon.interleukin and tumor necrosis factor. In recent years.non-mammalian vertebrate cytokines research is becoming increasingly important factor.especially in the economic value of aquatic animals cytokines.Cytokines are a class of biologically active substances.which are secreted by immune cells and non-immune cells. As signal transduction molecules between cells can be antigen antibodies and other information to the antigen receptor to mediate inflammation;As signal transduction molecules between cells.The antigen information can be passed to the antibodies and other antigen receptor to mediate inflammatory response;At the same time.they also regulate the immune response.stimulate of hematopoietic function and participate in tissue repair and so on. Interleukin-1(3 is a potential humoral immune response activator, which plays a key role in the local and systemic inflammatory response. It is generated mainly by blood monocytes and tissue macrophages. Interleukin-1βcan promote lymphocyte,macrophage, NK cell activation. also is a chemical inducer of neutrophil and mononuclear cells and involved in TH2 immune response.Interleukin-1βas a potential inflammatory mediators and costimulatory signal molecules,can increase macrophage phagocytosis.induce fever and promote the synthesis of prostaglandins.regulate the expression of other cytokines.As an immune regulatory cytokine, it has the potential to enhance the immune response.
In this study, we obtained the nucleic acid probe with carp IL-1βcDNA fragments of by fluorescence mRNA differential display.After digoxigenin labeled. application of nucleic acid hybridization screening mitogen stimulated peripheral blood leukocyte cDNA library of carp, we acquire carp IL-1(3 cDNA sequence.According to sequence non-translated region and exon of IL-1βcDNA the pair of primers were designed.IL-1βgenomic DNA sequences was obtained by the PCR.Finally,on the basis of the IL-1βcDNA and genomic DNA sequences the pair of primers were designed.by Real time quantitative PCR method, analysis of isolated and cultured in the normal and mitogen stimulation leucocytes in the transcription of the gene difference between the situation at different times. The results showed that: we get the carp IL-1βcDNA clone full-length 1213bp. containing the 5' and 3' untranslated region and 3' poly (A) polyadenylation signal (AATAAA), Open reading frame size of 831bp, encoding 276 amino acids,the homology of the deduced protein sequence to Carassius auratus.zebrafish.mice,cattle,human IL-1βwere 71%.54%, 22%,24% and 24%. Genomic DNA length is 2451 bp,sequence and phylogenetic analysis,the gene evolutionary is conservation, as with most species contain 7 exons and 6 introns,number of bases of each exon is close to mammalian exons correspondingly,exon and intron splice sites are complied with GT-AG rules. Quantitative PCR analysis showed that Mitogen-stimulated leukocytes increased transcription of IL-1β,especially the enhanced result of joint stimulation from LPS and PHA is particularly significant, but gradually decreased in stimulated leucocytes as the stimulation time going.It suggests IL-1βtranscription is different sensitivities to different inducers,also shows that the leucocytes play a immune role by early stimulating.
At present,the higher animals have been on the role of cytokines and their insight into the mechanism, and some cytokines can be used in clinical treatment.such as interferon.interleukin and tumor necrosis factor. In recent years.non-mammalian vertebrate cytokines research is becoming increasingly important factor.especially in the economic value of aquatic animals cytokines.Cytokines are a class of biologically active substances.which are secreted by immune cells and non-immune cells. As signal transduction molecules between cells can be antigen antibodies and other information to the antigen receptor to mediate inflammation;As signal transduction molecules between cells.The antigen information can be passed to the antibodies and other antigen receptor to mediate inflammatory response;At the same time.they also regulate the immune response.stimulate of hematopoietic function and participate in tissue repair and so on. Interleukin-1(3 is a potential humoral immune response activator, which plays a key role in the local and systemic inflammatory response. It is generated mainly by blood monocytes and tissue macrophages. Interleukin-1βcan promote lymphocyte,macrophage, NK cell activation. also is a chemical inducer of neutrophil and mononuclear cells and involved in TH2 immune response.Interleukin-1βas a potential inflammatory mediators and costimulatory signal molecules,can increase macrophage phagocytosis.induce fever and promote the synthesis of prostaglandins.regulate the expression of other cytokines.As an immune regulatory cytokine, it has the potential to enhance the immune response.
In this study, we obtained the nucleic acid probe with carp IL-1βcDNA fragments of by fluorescence mRNA differential display.After digoxigenin labeled. application of nucleic acid hybridization screening mitogen stimulated peripheral blood leukocyte cDNA library of carp, we acquire carp IL-1(3 cDNA sequence.According to sequence non-translated region and exon of IL-1βcDNA the pair of primers were designed.IL-1βgenomic DNA sequences was obtained by the PCR.Finally,on the basis of the IL-1βcDNA and genomic DNA sequences the pair of primers were designed.by Real time quantitative PCR method, analysis of isolated and cultured in the normal and mitogen stimulation leucocytes in the transcription of the gene difference between the situation at different times. The results showed that: we get the carp IL-1βcDNA clone full-length 1213bp. containing the 5' and 3' untranslated region and 3' poly (A) polyadenylation signal (AATAAA), Open reading frame size of 831bp, encoding 276 amino acids,the homology of the deduced protein sequence to Carassius auratus.zebrafish.mice,cattle,human IL-1βwere 71%.54%, 22%,24% and 24%. Genomic DNA length is 2451 bp,sequence and phylogenetic analysis,the gene evolutionary is conservation, as with most species contain 7 exons and 6 introns,number of bases of each exon is close to mammalian exons correspondingly,exon and intron splice sites are complied with GT-AG rules. Quantitative PCR analysis showed that Mitogen-stimulated leukocytes increased transcription of IL-1β,especially the enhanced result of joint stimulation from LPS and PHA is particularly significant, but gradually decreased in stimulated leucocytes as the stimulation time going.It suggests IL-1βtranscription is different sensitivities to different inducers,also shows that the leucocytes play a immune role by early stimulating.
引文
[1]Dinarello C. lnterleukin-1. Cytokine Growth Factor Rev1997;8:253-65.
[2]S.A. Loddick, C. Liu, T. Takao, K. Hashimoto, E.B. De Souza. Interleukin-1 receptors: cloning studies and role in central nervous system disorders, Brain Res. Brain Res. Rev.26 (1998)306-319.
[3]C.A. Dinarello. Biologic basis for interleukin-1 in disease.Blood 87 (1996) 2095-2147.
[4]Ellsaesser,C.F.&Clem,L.W.(1994).Functionally distinct high and low molecular weight species of channel catfish and mouse IL-1.Cytokine 6,10-20
[5]Verburg van Kemenade,B.M.L.,Weyts,F.A.A.,Debets.R.&Flik.G.(1995).Carp macrophages and neutrophilic granulocytes secrete an interleukin-1-like factor.Developmental and Comparative Immunology 19,59-70.
[6]Beuseher H,Gunther C,Rollinghoff M.IL-1 beta is secreted by activated murine macrophages as biologically inactive precursor.J Imunol 1990; 144:2179-83.
[7]Wieczorek M.Various aspects of IL-1 biological activity. Ⅱ.IL-1beta in diseases and the Central Nervous System. Wiad Parazytol.2009;55(2):115-24.
[8]Dinarello CA. The biology of interleukin-1. Chem Immunol 1992:51:1-32.
[9]Berkenbosch F, de Goeij DE, Rey AD, Besedovsky HO.Neuroendocrine, sympathetic and metabolic responses induced by interleukin-1. Neuroendocrinology 1989;50:570-6.
[10]Loddick SA, Liu C. Takao T, Hashimoto K. De Souza EB.Interleukin-1 receptors:cloning studies and role in central nervous system disorders. Brain Res Brain Res Rev 1998:26:306-19.
[11]Sims JE, March CJ. Cosman D, et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 1988;241:585-9.
[12]Auron PE. The interleukin-1 receptor:ligand interactions and signal transduction. Cytokine Growth Factor Rev 1998;9:221-37.
[13]Einstein R, et al. Type Ⅰ IL-1 receptor:ligand specific confirmation differences and the role of glycosylation in ligand binding. Cytokine 1996:8:206-13.
[14]Vigers GP. Anderson LJ. Caffes P. Brandhuber BJ. Crystal structure of the type-1 interleukin-1 receptor complexed with interleukin-1beta. Nature 1997:386:190-4.
[15]Martin M, Resch K. Interleukin 1:more than a mediator between leukocytes. Trends Pharmacol Sci 1988:9:171-7.
[16]Osborn L, Kunkel S, Nabel GJ. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc Natl Acad Sci USA 1989:86:2336-40.
[17]O'Neill LA, Dinarello CA. The IL-1 receptor/toll-like receptor superfamily:crucial receptors for inflammation and host defence. Immunol Today 2000; 21:206-9.
[18]Arend WP, Malyak M. Smith Jr MF, et al. Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids. J Immunol 1994;153:4766-74.
[19]Ghivizzani SC, Lechman ER, Kang R, et al. Direct adenovirus-mediated gene transfer of interleukin 1 and tumor necrosis factor alpha soluble receptors to rabbit knees with experimental arthritis has local and distal anti-arthritic effects. Proc Natl Acad Sci USA 1998:95:4613-3.
[20]Sims JE, Painter SL, Gow IR. Genomic organization of the type Ⅰ and type Ⅱ IL-1 receptors. Cytokine 1995;7:483-90.
[21]McMahan CJ, Slack JL, Mosley B, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J 1991:10:2821-32.
[22]Colotta F, Re F, Muzio M, et al. Interleukin-1 type Ⅱ receptor:a decoy target for IL-1 that is regulated by IL-4.Science 1993:261:472-5.
[23]Symons JA, Young PR, Duff GW. Soluble type Ⅱ interleukin 1 (IL-1) receptor binds and blocks processing of IL-1 beta precursor and loses affinity for IL-1 receptor antagonist. ProcNatl Acad Sci USA 1995;92:1714-8.
[24]Kollewe C, Neumann D, Martin MU. The first two Nterminal immunoglobulin-like domains of soluble human IL-1 receptor type Ⅱ are sufficient to bind and neutralize IL-1beta. FEBS Lett 2000:487:189-93.
[25]Lang D, Knop J. Wesche H. et al. The type Ⅱ IL-1 receptor interacts with the IL-1 receptor accessory protein:a novel mechanism of regulation of IL-1 responsiveness. J Immunol 1998:161:6871-7.
[26]Greenfeder SA. Nunes P. Kwee L. Labow M. Chizzonite RA.Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem 1995:270:13757-65.
[27]Dale M, Hammond DW, Cox A, Nicklin MJ. The human gene encoding the interleukin-1 receptor accessory protein (IL1RAP) maps to chromosome 3q28 by fluorescence in situ hybridization and radiation hybrid mapping. Genomics 1998:47:325-6.
[28]Cullinan EB. Kwee L, Nunes P, et al. IL-1 receptor accessory protein is an essential component of the IL-1 receptor.J Immunol 1998:161:5614-20.
[29]Jensen LE, Muzio M. Mantovani A, Whitehead AS. IL-1 signaling cascade in liver cells and the involvement of a soluble form of the IL-1 receptor accessory protein.J Immunol 2000; 164:5277-86.
[30]Hannum C, Wilcox C, Arend W, Joslin F, Dripps D, Heimdal P, et al. Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor. Nature 1990;343:336-40.
[31]Arend W. Interleukin-1 receptor antagonist. Adv Immunol 1993;54:167-227.
[32]Haskill S, Martin G, Vanle L, Morris J, Peace A, Bigler C, et al.cDNA cloning of an intracellular form of the human interleukin-1 receptor antagonist associated with epithelium. Proc Natl Acad Sci USA 1991;88:3681-5.
[33]Horai R, Saijo S, Tanioka H, et al. Development of chronic inflammatory arthropathy resembling rheumatoid arthritis in interleukin 1 receptor antagonist-deficient mice. J Exp Med 2000,191:313-320.
[34]Nicklin MJH. Hughes DE, Barton JL, Ure JM, Duff GW. Arterial inflammation in mice lacking the interleukin 1 receptor antagonist gene. J Exp Med 2000,191:303-311.
[35]C.A. Dinarello. Biologic basis for interleukin-1 in disease. Blood 87 (1996) 2095-2147.
[36]L. Vitkovic. J. Bockaert, C. Jacque."Inflammatory" cytokines:neuromodulators in normal brain. J Neurochem,74 (2000) 457-471.
[37]A. Avital,I. Goshen, A. Kamsler, M. Segal, K. Iverfeldt, G. Richter-Levin. R.Yirmiya. Impaired interleukin-1 signaling is associated with deficits in hippocampal memory processes and neural plasticity. Hippocampus 13(2003) 826-834.
[38]S.F. Maier, L.R. Watkins. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior mood, and cognition, Psychol. Rev.105(1998)83-107.
[39]W.A. Banks, A.J. Kastin. R.D. Broadwell. Passage of cytokines across the blood-brain barrier. Neuroimmunomodulation 2 (1995) 241-248.
[40]E.W. Bernton, J.E. Beach, J.W. Holaday, R.C. Smallridge, H.G. Fein.Release of multiple hormones by a direct action of interleukin-1 on pituitary cells. Science 238 (1987) 519-521.
[41]M.H. Whitnall, E. Mezey, H. Gainer. Co-localization of corticotropin-releasing factor and vasopressin in median eminence neurosecretory vesicles. Nature 317 (1985) 248-250.
[42]R. Landgraf, I. Neumann, F. Holsboer, Q.J. Pittman. Interleukin-1 beta stimulates both central and peripheral release of vasopressin and oxytocin in the rat. Eur. J. Neurosci.7 (1995) 592-598.
[43]Y. Amano, S.W. Lee, A.C. Allison. Inhibition by glucocorticoids of the formation of interleukin-1 alpha, interleukin-1 beta, and interleukin-6:mediation by decreased mRNA stability. Mol. Pharmacol.43 (1993) 176-182.
[44]P.J. Knudsen, C.A. Dinarello, T.B. Strom.Glucocorticoids inhibit transcriptional and post-transcriptional expression of interleukin 1 in U937 cells. J. Immunol.139 (1987) 4129-4134.
[45]T. Ben-Hur, R. Cialic, A. Itzik, O. Barak, R. Yirmiya, J. Weidenfeld. A novel permissive role for glucocorticoids in induction of febrile and behavioral signs of experimental herpes simplex virus encephalitis. Neuroscience 108(2001) 119-127.
[46]E. Goujon, P. Parnet, S. Laye, C. Combe, K.W. Kelley, R. Dantzer.Stress downregulates lipopolysaccharide-induced expression of proinflammatory cytokines in the spleen, pituitary. and brain of mice. Brain Behav. Immun.9(1995) 292-303.
[47]S.F. Maier.Bi-directional immune-brain communication:implications for understanding stress, pain, and cognition. Brain Behav. Immun.17 (2003)69-85.
[48]D. Wittwer, A. Franchini, E. Ottaviani, A. Wiesner.Presence of IL-1 and TNFlike molecules in Galleria mellonella (Lepidoptera) haemocytes and in an insect cell line Fromestigmene acraea (Lepidoptera). Cytokine 11 (1999) 637-642.
[49]E. Ottaviani, A. Franchini, C. Franceschi.Presence of several cytokine-like molecules in molluscan hemocytes.Biochem. Biophys. Res. Commun.195(1993) 984-988.
[50]E. Ottaviani, C. Franceschi, The neuroimmunology of stress from invertebrates to man. Prog. Neurobiol.48 (1996) 421-440.
[51]Palsson EM,Popoff M,Thelestam M, et al.Divergent roles for Ras and Rap in the activation of P38 mitogen-activated protein kinase by interleukin-l.J Biol Chem.2000,275,7818-7825
[52]IizumiT.Sato S,Iiyama T,Hata R.Amemiya H.Tomomasa H.Yazaki T.Umeda T. Recombinant human interleukin-1 bete analogue as a regulartor of hematopoiesis in patients receiving chemotherapy for urogenital cancers. Cancer 1991;68:1520-1523
[53]Anju S.Moolwaney and Orisa J. Igwe. Regulation of the cyclooxygenase-2 system by interleukin-1β through mitogen-activated protein kinase signaling pathways:A comparative study of human neuroglioma and neuroblastoma cells.Brain Res Mol Brain Res,2005.137 (1-2):202-212.
[54]Javier Duque.ManuelD.Diaz-Munoz. Manuel Fresno and Miguel A.Iniguez.UP-regulation of cyclooxygenase-2 by interleukin-1β in colon carcinoma cells. Cellular Signal,2006,18(8), 1262-1269.
[55]Partha MukhoPadhyay M.Aktar Ali, Animesh Nandi,Peter Carreon,Hak Choy and Debabrata Saha. The Cyclin-Dependent Kinase2 Inhibitor Down-regulates Interleukin-1β-Mediated Induction of Cyclooxygenase-2 ExPression in Human Lung.Carcinoma Cells Cancer Res 2006:66(3):1758-66.
[56]Yun-Jin Jung,Jennifer S.lsaacs,Sunmin Lee, Jane Trepel.and Len Neckers.IL-1β mediated up-regulation of HIF-lαvia an NF-κB/COX-2 pathway identifies HIF-1 as a critical link between inflammation and oncogenesis.The FASEB Journal Express Article 2003,17(14): 2115-211.
[57]Charles A.Dinarello. Biologic basis for interleukin-1 in disease.Blood 87:2095.1996.
[58]WetZler M.kurzrock R,Lave DG,et al.Alteration in bone marrow adherent layer factor expression:a novel mechanism of chronic myelogenous leukemia progression. Blood,78:2400,1991.
[59]周荣富等.白血病患者血清1L-1β水平的测定及意义.10(3)：134,2001.
[60]Ferrari C, Pott GodoyM. Tarelli R,et al. Progressive neurodegeneration and motor disabilities induced by chronic expression of IL-lbeta in the substantia nigra[J]. Neurobiol Dis.2006.24: 183-193.
[61]Barnum CJ, Eskow KL, Dupre K.et al. Exogenous corticosterone reduces L-Dopa-induced dyskinesia in the hemiparkinsonian rat role for interleukin-1[J]. Neuroscience,2008,156: 30-41.
[62]Vroon A, Drukarch B, Bol JG,et al. Neuroinflammation in Parkinson' s patients and MPTP-treated mice is not restricted to the nigrostriatal system:Microgliosis and differential expression of interleukin-1 receptors in the olfactory bulb[J]. Exp Gerontol,2007.42: 762-771.
[63]Yi Peilu, Tsai Chon-Haw, LuMingkuei,et al. Interleukin-1beta mediates sleep alteration in rats with rotenone-induced parkinsonism[J]. Sleep,2007,30:413-425.
[64]王茸,王洪,李正求等.支气管哮喘患儿急性发作期血清IL-1p和IL-4水平的检测.细胞与分子免疫学学杂志,2001,17.
[65]丰培金.鲤白细胞cDNA文库的构建及免疫应答相关基因的克隆[D].长春：中国人民解放军军需大学.2004,69-71.
[66]卢强,丰培金,李莲瑞等.有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库的构建[J].中国兽医学报,2004，24(6)：568-570.
[67]付保忠.鲤鱼蛋白酶体激活因子PA28-β亚单位基因克隆、鉴定及时空转录分析[D].兰州：甘肃农业大学,2008.
[68]Zou J. Cunningham C, Secombes C. The rainbow trout Oncorhynchus mykiss interleukin-1βgene has a different organisation to mammals and undergoes incomplete splicing. Eur J Biochem 1999:259:901-8.
[69]Trotman C. Introns early:slipping lately? Trends Genet 1998; 14:132-4.
[70]Gilbert W, De Souza S. Long M. Origin of genes. Proc Natl Acad Sci USA 1997;94:7698-703.
[71]Nielsen H, Engelbrecht J, Brunak S, von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 1997:10:1-6.
[72]Sizemore RC, Miller NW, Cuchens MA. et al. Phylogeny of lymphocyte heterogeneity: the cellular requirements for in vitro mitogenic responses of channel catfish leukocytes. J Immunol.1984.133:2920-2924.
[73]谭业平.鲤鱼蛋白酶体激活因子PA28-α亚单位基闪克隆、鉴定及时空转录分析[D].长春：吉林大学,2007.
[74]李莲瑞.鲤鱼(Cyprinus carpio L.)外周血白细胞cDNA文库的构建及差异表达基因的筛选[D].长春：吉林大学,2006.
[75]Weining K, Sick C, Kaspers B, Staeheli P. A chicken homolog of mammalian interleukin-1 beta:cDNA cloning and purification of active recombinant protein. Eur J Biochem 1998;258:994-1000.
[76]Zou J, Bird S, Minter R, Horton J, Cunningham C, Secombes C. Molecular cloning of the gene for interleukin-1βfrom Xenopus laevis and analysis of expression in vivo and in vitro. Immunogenetics 2000;49:332-8.
[77]Engelsma M, Stet R, Schipper H, Verburg van Kemenade B. Regulation of interleukin-1 beta RNA expression in the common carp. Cyprinus carpio L. Dev Comp Immunol 2001;25:195-203.
[78]Zou J, Holland J, Pleguezuelos O, Cunningham C, Secombes CJ. Factors influencing the expression of interleukin-1 beta in cultured rainbow trout (Oncorhynchus mykiss) leucocytes. Dev Comp Immunol 2000;24:575-82.
[79]Scapigliati G, Buonocore F, Bird S, Zou J, Pelegrin P, Falasca C. et al. Phylogeny of cytokines:molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta. Fish Shellfish Immunol 2001;11:711-26.
[80]Bird S, Wang T, Zou J, Cunningham C, Secombes C. The first cytokine sequence within cartilaginous fish:interleukin-1β in the small spotted catshark (Scyliorhinus canicula). J Immunol 2000;168(7):3329-40.
[81]Wang T, Zou J, Cunningham C, Secombes C. Cloning and functional characterisation of the interleukin-1 promoter of rainbow trout (Oncorhynchus mykiss). Biochem Biophys Acta 2002; 1575(1-3):108-16.
[2]S.A. Loddick, C. Liu, T. Takao, K. Hashimoto, E.B. De Souza. Interleukin-1 receptors: cloning studies and role in central nervous system disorders, Brain Res. Brain Res. Rev.26 (1998)306-319.
[3]C.A. Dinarello. Biologic basis for interleukin-1 in disease.Blood 87 (1996) 2095-2147.
[4]Ellsaesser,C.F.&Clem,L.W.(1994).Functionally distinct high and low molecular weight species of channel catfish and mouse IL-1.Cytokine 6,10-20
[5]Verburg van Kemenade,B.M.L.,Weyts,F.A.A.,Debets.R.&Flik.G.(1995).Carp macrophages and neutrophilic granulocytes secrete an interleukin-1-like factor.Developmental and Comparative Immunology 19,59-70.
[6]Beuseher H,Gunther C,Rollinghoff M.IL-1 beta is secreted by activated murine macrophages as biologically inactive precursor.J Imunol 1990; 144:2179-83.
[7]Wieczorek M.Various aspects of IL-1 biological activity. Ⅱ.IL-1beta in diseases and the Central Nervous System. Wiad Parazytol.2009;55(2):115-24.
[8]Dinarello CA. The biology of interleukin-1. Chem Immunol 1992:51:1-32.
[9]Berkenbosch F, de Goeij DE, Rey AD, Besedovsky HO.Neuroendocrine, sympathetic and metabolic responses induced by interleukin-1. Neuroendocrinology 1989;50:570-6.
[10]Loddick SA, Liu C. Takao T, Hashimoto K. De Souza EB.Interleukin-1 receptors:cloning studies and role in central nervous system disorders. Brain Res Brain Res Rev 1998:26:306-19.
[11]Sims JE, March CJ. Cosman D, et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 1988;241:585-9.
[12]Auron PE. The interleukin-1 receptor:ligand interactions and signal transduction. Cytokine Growth Factor Rev 1998;9:221-37.
[13]Einstein R, et al. Type Ⅰ IL-1 receptor:ligand specific confirmation differences and the role of glycosylation in ligand binding. Cytokine 1996:8:206-13.
[14]Vigers GP. Anderson LJ. Caffes P. Brandhuber BJ. Crystal structure of the type-1 interleukin-1 receptor complexed with interleukin-1beta. Nature 1997:386:190-4.
[15]Martin M, Resch K. Interleukin 1:more than a mediator between leukocytes. Trends Pharmacol Sci 1988:9:171-7.
[16]Osborn L, Kunkel S, Nabel GJ. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc Natl Acad Sci USA 1989:86:2336-40.
[17]O'Neill LA, Dinarello CA. The IL-1 receptor/toll-like receptor superfamily:crucial receptors for inflammation and host defence. Immunol Today 2000; 21:206-9.
[18]Arend WP, Malyak M. Smith Jr MF, et al. Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids. J Immunol 1994;153:4766-74.
[19]Ghivizzani SC, Lechman ER, Kang R, et al. Direct adenovirus-mediated gene transfer of interleukin 1 and tumor necrosis factor alpha soluble receptors to rabbit knees with experimental arthritis has local and distal anti-arthritic effects. Proc Natl Acad Sci USA 1998:95:4613-3.
[20]Sims JE, Painter SL, Gow IR. Genomic organization of the type Ⅰ and type Ⅱ IL-1 receptors. Cytokine 1995;7:483-90.
[21]McMahan CJ, Slack JL, Mosley B, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J 1991:10:2821-32.
[22]Colotta F, Re F, Muzio M, et al. Interleukin-1 type Ⅱ receptor:a decoy target for IL-1 that is regulated by IL-4.Science 1993:261:472-5.
[23]Symons JA, Young PR, Duff GW. Soluble type Ⅱ interleukin 1 (IL-1) receptor binds and blocks processing of IL-1 beta precursor and loses affinity for IL-1 receptor antagonist. ProcNatl Acad Sci USA 1995;92:1714-8.
[24]Kollewe C, Neumann D, Martin MU. The first two Nterminal immunoglobulin-like domains of soluble human IL-1 receptor type Ⅱ are sufficient to bind and neutralize IL-1beta. FEBS Lett 2000:487:189-93.
[25]Lang D, Knop J. Wesche H. et al. The type Ⅱ IL-1 receptor interacts with the IL-1 receptor accessory protein:a novel mechanism of regulation of IL-1 responsiveness. J Immunol 1998:161:6871-7.
[26]Greenfeder SA. Nunes P. Kwee L. Labow M. Chizzonite RA.Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem 1995:270:13757-65.
[27]Dale M, Hammond DW, Cox A, Nicklin MJ. The human gene encoding the interleukin-1 receptor accessory protein (IL1RAP) maps to chromosome 3q28 by fluorescence in situ hybridization and radiation hybrid mapping. Genomics 1998:47:325-6.
[28]Cullinan EB. Kwee L, Nunes P, et al. IL-1 receptor accessory protein is an essential component of the IL-1 receptor.J Immunol 1998:161:5614-20.
[29]Jensen LE, Muzio M. Mantovani A, Whitehead AS. IL-1 signaling cascade in liver cells and the involvement of a soluble form of the IL-1 receptor accessory protein.J Immunol 2000; 164:5277-86.
[30]Hannum C, Wilcox C, Arend W, Joslin F, Dripps D, Heimdal P, et al. Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor. Nature 1990;343:336-40.
[31]Arend W. Interleukin-1 receptor antagonist. Adv Immunol 1993;54:167-227.
[32]Haskill S, Martin G, Vanle L, Morris J, Peace A, Bigler C, et al.cDNA cloning of an intracellular form of the human interleukin-1 receptor antagonist associated with epithelium. Proc Natl Acad Sci USA 1991;88:3681-5.
[33]Horai R, Saijo S, Tanioka H, et al. Development of chronic inflammatory arthropathy resembling rheumatoid arthritis in interleukin 1 receptor antagonist-deficient mice. J Exp Med 2000,191:313-320.
[34]Nicklin MJH. Hughes DE, Barton JL, Ure JM, Duff GW. Arterial inflammation in mice lacking the interleukin 1 receptor antagonist gene. J Exp Med 2000,191:303-311.
[35]C.A. Dinarello. Biologic basis for interleukin-1 in disease. Blood 87 (1996) 2095-2147.
[36]L. Vitkovic. J. Bockaert, C. Jacque."Inflammatory" cytokines:neuromodulators in normal brain. J Neurochem,74 (2000) 457-471.
[37]A. Avital,I. Goshen, A. Kamsler, M. Segal, K. Iverfeldt, G. Richter-Levin. R.Yirmiya. Impaired interleukin-1 signaling is associated with deficits in hippocampal memory processes and neural plasticity. Hippocampus 13(2003) 826-834.
[38]S.F. Maier, L.R. Watkins. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior mood, and cognition, Psychol. Rev.105(1998)83-107.
[39]W.A. Banks, A.J. Kastin. R.D. Broadwell. Passage of cytokines across the blood-brain barrier. Neuroimmunomodulation 2 (1995) 241-248.
[40]E.W. Bernton, J.E. Beach, J.W. Holaday, R.C. Smallridge, H.G. Fein.Release of multiple hormones by a direct action of interleukin-1 on pituitary cells. Science 238 (1987) 519-521.
[41]M.H. Whitnall, E. Mezey, H. Gainer. Co-localization of corticotropin-releasing factor and vasopressin in median eminence neurosecretory vesicles. Nature 317 (1985) 248-250.
[42]R. Landgraf, I. Neumann, F. Holsboer, Q.J. Pittman. Interleukin-1 beta stimulates both central and peripheral release of vasopressin and oxytocin in the rat. Eur. J. Neurosci.7 (1995) 592-598.
[43]Y. Amano, S.W. Lee, A.C. Allison. Inhibition by glucocorticoids of the formation of interleukin-1 alpha, interleukin-1 beta, and interleukin-6:mediation by decreased mRNA stability. Mol. Pharmacol.43 (1993) 176-182.
[44]P.J. Knudsen, C.A. Dinarello, T.B. Strom.Glucocorticoids inhibit transcriptional and post-transcriptional expression of interleukin 1 in U937 cells. J. Immunol.139 (1987) 4129-4134.
[45]T. Ben-Hur, R. Cialic, A. Itzik, O. Barak, R. Yirmiya, J. Weidenfeld. A novel permissive role for glucocorticoids in induction of febrile and behavioral signs of experimental herpes simplex virus encephalitis. Neuroscience 108(2001) 119-127.
[46]E. Goujon, P. Parnet, S. Laye, C. Combe, K.W. Kelley, R. Dantzer.Stress downregulates lipopolysaccharide-induced expression of proinflammatory cytokines in the spleen, pituitary. and brain of mice. Brain Behav. Immun.9(1995) 292-303.
[47]S.F. Maier.Bi-directional immune-brain communication:implications for understanding stress, pain, and cognition. Brain Behav. Immun.17 (2003)69-85.
[48]D. Wittwer, A. Franchini, E. Ottaviani, A. Wiesner.Presence of IL-1 and TNFlike molecules in Galleria mellonella (Lepidoptera) haemocytes and in an insect cell line Fromestigmene acraea (Lepidoptera). Cytokine 11 (1999) 637-642.
[49]E. Ottaviani, A. Franchini, C. Franceschi.Presence of several cytokine-like molecules in molluscan hemocytes.Biochem. Biophys. Res. Commun.195(1993) 984-988.
[50]E. Ottaviani, C. Franceschi, The neuroimmunology of stress from invertebrates to man. Prog. Neurobiol.48 (1996) 421-440.
[51]Palsson EM,Popoff M,Thelestam M, et al.Divergent roles for Ras and Rap in the activation of P38 mitogen-activated protein kinase by interleukin-l.J Biol Chem.2000,275,7818-7825
[52]IizumiT.Sato S,Iiyama T,Hata R.Amemiya H.Tomomasa H.Yazaki T.Umeda T. Recombinant human interleukin-1 bete analogue as a regulartor of hematopoiesis in patients receiving chemotherapy for urogenital cancers. Cancer 1991;68:1520-1523
[53]Anju S.Moolwaney and Orisa J. Igwe. Regulation of the cyclooxygenase-2 system by interleukin-1β through mitogen-activated protein kinase signaling pathways:A comparative study of human neuroglioma and neuroblastoma cells.Brain Res Mol Brain Res,2005.137 (1-2):202-212.
[54]Javier Duque.ManuelD.Diaz-Munoz. Manuel Fresno and Miguel A.Iniguez.UP-regulation of cyclooxygenase-2 by interleukin-1β in colon carcinoma cells. Cellular Signal,2006,18(8), 1262-1269.
[55]Partha MukhoPadhyay M.Aktar Ali, Animesh Nandi,Peter Carreon,Hak Choy and Debabrata Saha. The Cyclin-Dependent Kinase2 Inhibitor Down-regulates Interleukin-1β-Mediated Induction of Cyclooxygenase-2 ExPression in Human Lung.Carcinoma Cells Cancer Res 2006:66(3):1758-66.
[56]Yun-Jin Jung,Jennifer S.lsaacs,Sunmin Lee, Jane Trepel.and Len Neckers.IL-1β mediated up-regulation of HIF-lαvia an NF-κB/COX-2 pathway identifies HIF-1 as a critical link between inflammation and oncogenesis.The FASEB Journal Express Article 2003,17(14): 2115-211.
[57]Charles A.Dinarello. Biologic basis for interleukin-1 in disease.Blood 87:2095.1996.
[58]WetZler M.kurzrock R,Lave DG,et al.Alteration in bone marrow adherent layer factor expression:a novel mechanism of chronic myelogenous leukemia progression. Blood,78:2400,1991.
[59]周荣富等.白血病患者血清1L-1β水平的测定及意义.10(3)：134,2001.
[60]Ferrari C, Pott GodoyM. Tarelli R,et al. Progressive neurodegeneration and motor disabilities induced by chronic expression of IL-lbeta in the substantia nigra[J]. Neurobiol Dis.2006.24: 183-193.
[61]Barnum CJ, Eskow KL, Dupre K.et al. Exogenous corticosterone reduces L-Dopa-induced dyskinesia in the hemiparkinsonian rat role for interleukin-1[J]. Neuroscience,2008,156: 30-41.
[62]Vroon A, Drukarch B, Bol JG,et al. Neuroinflammation in Parkinson' s patients and MPTP-treated mice is not restricted to the nigrostriatal system:Microgliosis and differential expression of interleukin-1 receptors in the olfactory bulb[J]. Exp Gerontol,2007.42: 762-771.
[63]Yi Peilu, Tsai Chon-Haw, LuMingkuei,et al. Interleukin-1beta mediates sleep alteration in rats with rotenone-induced parkinsonism[J]. Sleep,2007,30:413-425.
[64]王茸,王洪,李正求等.支气管哮喘患儿急性发作期血清IL-1p和IL-4水平的检测.细胞与分子免疫学学杂志,2001,17.
[65]丰培金.鲤白细胞cDNA文库的构建及免疫应答相关基因的克隆[D].长春：中国人民解放军军需大学.2004,69-71.
[66]卢强,丰培金,李莲瑞等.有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库的构建[J].中国兽医学报,2004，24(6)：568-570.
[67]付保忠.鲤鱼蛋白酶体激活因子PA28-β亚单位基因克隆、鉴定及时空转录分析[D].兰州：甘肃农业大学,2008.
[68]Zou J. Cunningham C, Secombes C. The rainbow trout Oncorhynchus mykiss interleukin-1βgene has a different organisation to mammals and undergoes incomplete splicing. Eur J Biochem 1999:259:901-8.
[69]Trotman C. Introns early:slipping lately? Trends Genet 1998; 14:132-4.
[70]Gilbert W, De Souza S. Long M. Origin of genes. Proc Natl Acad Sci USA 1997;94:7698-703.
[71]Nielsen H, Engelbrecht J, Brunak S, von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 1997:10:1-6.
[72]Sizemore RC, Miller NW, Cuchens MA. et al. Phylogeny of lymphocyte heterogeneity: the cellular requirements for in vitro mitogenic responses of channel catfish leukocytes. J Immunol.1984.133:2920-2924.
[73]谭业平.鲤鱼蛋白酶体激活因子PA28-α亚单位基闪克隆、鉴定及时空转录分析[D].长春：吉林大学,2007.
[74]李莲瑞.鲤鱼(Cyprinus carpio L.)外周血白细胞cDNA文库的构建及差异表达基因的筛选[D].长春：吉林大学,2006.
[75]Weining K, Sick C, Kaspers B, Staeheli P. A chicken homolog of mammalian interleukin-1 beta:cDNA cloning and purification of active recombinant protein. Eur J Biochem 1998;258:994-1000.
[76]Zou J, Bird S, Minter R, Horton J, Cunningham C, Secombes C. Molecular cloning of the gene for interleukin-1βfrom Xenopus laevis and analysis of expression in vivo and in vitro. Immunogenetics 2000;49:332-8.
[77]Engelsma M, Stet R, Schipper H, Verburg van Kemenade B. Regulation of interleukin-1 beta RNA expression in the common carp. Cyprinus carpio L. Dev Comp Immunol 2001;25:195-203.
[78]Zou J, Holland J, Pleguezuelos O, Cunningham C, Secombes CJ. Factors influencing the expression of interleukin-1 beta in cultured rainbow trout (Oncorhynchus mykiss) leucocytes. Dev Comp Immunol 2000;24:575-82.
[79]Scapigliati G, Buonocore F, Bird S, Zou J, Pelegrin P, Falasca C. et al. Phylogeny of cytokines:molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta. Fish Shellfish Immunol 2001;11:711-26.
[80]Bird S, Wang T, Zou J, Cunningham C, Secombes C. The first cytokine sequence within cartilaginous fish:interleukin-1β in the small spotted catshark (Scyliorhinus canicula). J Immunol 2000;168(7):3329-40.
[81]Wang T, Zou J, Cunningham C, Secombes C. Cloning and functional characterisation of the interleukin-1 promoter of rainbow trout (Oncorhynchus mykiss). Biochem Biophys Acta 2002; 1575(1-3):108-16.