籽鹅垂体组织全长cDNA文库的构建及部分克隆序列分析
|
摘要
为深入研究籽鹅垂体调控生殖生理的分子机理,以籽鹅垂体组织为试验材料,用SMART(switching mechanism at 5′end of RNA transcript)方法构建了籽鹅垂体组织的全长cDNA文库。随机挑取11个噬菌斑,用载体克隆位点两端的通用引物进行扩增,以检测所构建的文库的质量。将文库的λTriplEx2噬菌体载体转化到pTriplEx2质粒载体,随机挑取217个克隆,用T7和SP6通用引物进行PCR鉴定,并选取不同长度的基因143个测序。取得结果如下:
1.利用λTriplEx2噬菌体构建了籽鹅垂体组织cDNA文库,并对文库质量进行鉴定结果显示,该文库的初始滴度为6×105 pfu/mL,重组率为94%,插入片段长度范围为0.5～2.0 kb。结果表明,成功构建了籽鹅垂体组织cDNA文库。
2.选取不同长度143个克隆测序,完成测序140个,去掉冗余序列后共有108个序列,unigene比率为87%。测定的cDNA序列与BLASTn进行比较,其中73个与鸡具有较高的同源性,可认定为已知序列EST,其中1个与大雁的催乳素基因的相似性高达99%;1个与大雁的生长激素基因的相似性高达100%,还有1个与原鸡的铁蛋白重链基因的相似性达到90%。获得35个未注册基因。本研究为进一步从分子水平探讨籽鹅垂体调控生殖生理的分子机理奠定了基础。
In order to further study the molecular mechanism of reproductive physiology controlled by pituitary of Zi-goose. The experiment was conducted to constructe the full-length cDNA library from the pituitary gland of the Zi-goose (Anser anser) by SMART (switching mechanism at 5′end of RNA Transcript) technique, which usedλTriplEx2 as a vector. Thenλphage packaging reaction and library amplification were performed. Eleven plaques were randomly picked and tested using PCR method with universal primers derived from the sequence flanking the vector. Then convertingλTriplEx2 to pTriplEx2, 217 clones were randomly picked and tested using PCR with universal primers T7 and SP6, 143 clones were chosen at randomly for sequencing.
The results were as follows:
1. The full-length cDNA library of pituitary gland from Zi-goose was constructive withλTriplEx2 vector and the qualities of original cDNA library were strictly checked by conventional titer ditermination. The results showed that the titration of the cDNA library constructed was 6×105 pfu/mL, the rate of recombination was 94 %, and the fragment length of inserted DNA ranged mainly from 0.5 to 2.0kb. The library titer, recombination rate and average inserted size all match quality requirments for cDNA library and this indicated that cDNA library of pituitary gland was successfully constructed.
2. 143 clones were chosen at randomly for sequencing from the cDNA library, and 140 clones were sequenced successfully, 108 unigene weregained after unigene clusters. The ratio of unigene obtained was up to 87%. The BLAST sequence analysis indicated that 73 clones were known ESTs, and 35 clones were novel ESTs, and after BLASTn analysis of cDNAs, there possible function were predict. Through cross-species genenomic comparison, one of these are similar to the prolaction(PRL) gene of Anser anser ,homology was up to 99%; one of these are similar to the growth hormone(GH) of Anser anser, homology was up to 100%. one of these are similar to the ferritin heavy chain(FTH)of Gallus gallus, homology was up to 90%. This stady laid the foundation for further research on the mechanism of reproductive physiology controlled by pituitary of Zi-goose.
1.利用λTriplEx2噬菌体构建了籽鹅垂体组织cDNA文库,并对文库质量进行鉴定结果显示,该文库的初始滴度为6×105 pfu/mL,重组率为94%,插入片段长度范围为0.5～2.0 kb。结果表明,成功构建了籽鹅垂体组织cDNA文库。
2.选取不同长度143个克隆测序,完成测序140个,去掉冗余序列后共有108个序列,unigene比率为87%。测定的cDNA序列与BLASTn进行比较,其中73个与鸡具有较高的同源性,可认定为已知序列EST,其中1个与大雁的催乳素基因的相似性高达99%;1个与大雁的生长激素基因的相似性高达100%,还有1个与原鸡的铁蛋白重链基因的相似性达到90%。获得35个未注册基因。本研究为进一步从分子水平探讨籽鹅垂体调控生殖生理的分子机理奠定了基础。
In order to further study the molecular mechanism of reproductive physiology controlled by pituitary of Zi-goose. The experiment was conducted to constructe the full-length cDNA library from the pituitary gland of the Zi-goose (Anser anser) by SMART (switching mechanism at 5′end of RNA Transcript) technique, which usedλTriplEx2 as a vector. Thenλphage packaging reaction and library amplification were performed. Eleven plaques were randomly picked and tested using PCR method with universal primers derived from the sequence flanking the vector. Then convertingλTriplEx2 to pTriplEx2, 217 clones were randomly picked and tested using PCR with universal primers T7 and SP6, 143 clones were chosen at randomly for sequencing.
The results were as follows:
1. The full-length cDNA library of pituitary gland from Zi-goose was constructive withλTriplEx2 vector and the qualities of original cDNA library were strictly checked by conventional titer ditermination. The results showed that the titration of the cDNA library constructed was 6×105 pfu/mL, the rate of recombination was 94 %, and the fragment length of inserted DNA ranged mainly from 0.5 to 2.0kb. The library titer, recombination rate and average inserted size all match quality requirments for cDNA library and this indicated that cDNA library of pituitary gland was successfully constructed.
2. 143 clones were chosen at randomly for sequencing from the cDNA library, and 140 clones were sequenced successfully, 108 unigene weregained after unigene clusters. The ratio of unigene obtained was up to 87%. The BLAST sequence analysis indicated that 73 clones were known ESTs, and 35 clones were novel ESTs, and after BLASTn analysis of cDNAs, there possible function were predict. Through cross-species genenomic comparison, one of these are similar to the prolaction(PRL) gene of Anser anser ,homology was up to 99%; one of these are similar to the growth hormone(GH) of Anser anser, homology was up to 100%. one of these are similar to the ferritin heavy chain(FTH)of Gallus gallus, homology was up to 90%. This stady laid the foundation for further research on the mechanism of reproductive physiology controlled by pituitary of Zi-goose.
引文
[1]陈国宏,王克华,王金玉,等.中国禽类遗传资源.北京:中国教育出版社,2004.
[2]卢圣栋.现代分子生物学实验技术(第二版)[M].北京:中国协和医科大学,1999,319-328.
[3]沈倍奋.分子文库[M].北京:科学出版社,2001.
[4]黄英,张宗玉.cDNA文库构建策略[J].国外医学分子生物学分册,1999,21(6):337-340.
[5]董志敏,张宝石,关荣霞,等.全长cDNA文库的构建方法[J].中国农学通报,2006, 22(2): 51-55.
[6] Weissman SM.Molecular genetic techniques for mapping the human genome [J].Mol Biol Med, 1987, 4(3):133-143.
[7] Bonoldo MF, Lennon G, Soares MB.Normalization and Subtraction: Two approaches to facilitate gene discovery [J].Genomo Pes, 1996, 6: 791-806.
[8] Adjaye J, Daniels R, Monk.The construction of cDNA libraries from human single preimplantation embryos and their use in the study of gene expression during development [J].J Assist Reprod Genet, 1998, 15: 344-348.
[9] Vitek MP,Kreissman SG,Gross RH.The isolation of ecdysterone inducible genes by hybridization subtraction chromatography [J].Nucleic Acids Res, 1981, 9:1191-1202.
[10] Wang SM, Fears SC, Zhang L, et al.Screening poly(dAydT)+cDNAs for gene identification [J].Pro Natl Acad Sci USA, 2000, 97(8): 4162-4167.
[11] Sive HL,John TS.A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction[J].Neucleic Acid Res, 1988, 16:10937
[12] Sargent TD,Dawid IB.Differential gene expression in the Gastrula of Xenopus laevis[J].Science,1983, 222:135-139.
[13] Wang Z, Brown DD.A gene Expression Screen[J].Pro Natl Acad Sci USA, 1991, 88:15505-11509.
[14] Scbraml P,sbiPman R,Stulz P,et al.cDNA substraction library construction using a magnet-assisted substraction technique (MAST) [J].TIG, 1993, 9:70-71.
[15] Diatchenko L, Lau YFC, Campbell A P.Suppression Subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries[J].Proc Natl Sci USA, 1996, 93(12): 6025~6030.
[16] Siebert P D, Chenchik A, Kellogg D E.An improved PCR method for walking in uncloned genomic DNA[J].Nucleic Acids Res, 1995, 3:1087-1088.
[17] Sylvain Legrand, Theo Hendriks, Jean-Louis Hilbert, et al.Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L[J].BMC Plant Biology, 2007,7:27.
[18] Ho T Y,Hsiang C Y,Wu K,el al.Rapid screening of the pseudorabies virus-specific cDNAs from a cDNA library [J].J Virol Methods, 58:187-192.
[19] Thomas R.Solid phase cDNA construction,a versatile approach[J].Nucleic Acids Research, 1998, 26(14): 3451-3452.
[20]王义琴,李文彬.一种改进的cDNA文库固相构建法[J].遗传,2000, 22(4):253-254.
[21] Haas B J,Volfovsky N,Town CD,et al.Full-length messenger RNA sequences greatly improve genome annotation[J].Genome Biol,2002,3(6):RESEARCH0029.
[22] Salzberg SL,Pertea M,Delcher AL,et al.Interpolated Markov models for eukaryotic gene finding[J].Genomics,1999,59(l):24-31.
[23] Lukashin AV,Borodovsky M. GeneMark. Hmm.New solutions for gene finding [J].Nucleic Acids Res,1998,26(4):1107-1115.
[24] Burge CB,Karlin S.Prediction of complete gene structure in human genomic DNA [J].Mol Bio,1997,268:78-94.
[25] Kikuchi S,Satoh K,Nagata T,et al.Collection,mapping,and annotation of over 28,000 cDNA clones from japonica rice [J].Science,2003,301(5631):376-379.
[26] Carninci P,Kazunori Waki,Toshiyuki Shiraki,et al.Targeting a Complex Transcriptome:The Construction of the Mouse Full-Length cDNA Encyclopedia [J].Genome Res,2003,3:1273-1289.
[27] Seki M,Narusaka M,Kamiya A,et al.Functional annotation of a full-length Arabidopsis cDNA collection[J].Science,2002,296(5565) :141-145.
[28] Stapleton M,Liao GC,Carninci P,et al.The Drosophila Gene Collection: Identification of Putative Full-Length cDNAs for 70% of D. melanogaster Genes[J].GenomeRes,2002,12:1294-1300.
[29]吴伟,徐日福,高光,等.鹅肌肉组织全长cDNA文库构建及泛素Ⅰ基因序列分析[J].畜牧与兽医杂志,2007,39(5):12-15.
[30]徐日福,吴伟,高光.鹅肝脏组织的质粒cDNA文库构建.吉林农业大学学报[J].2007,29(3):325-328.
[31] Yen CF, et al.The Expression of Pituitary Gland Genes in Laying Geese[J].Poultry Science, 2006,8:2265-2269
[32] Adams M D, Kelley J M, Gocayne J D, et al.Complementary DNA Sequencing: Sequence Tags and Human Genome Project [J].Science, 1991, 252:1651-1656.
[33] Harushima Y, Yano M, Shomura A, et al.A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics [J].1998 January; 148(1):479-494.
[34] Brent A E, MacQueen A, Hazelrigg T.The Drosophila wispy gene is required for RNA localization and other microtubule-based events of meiosis and early embryogenesis [J].Genetics. 2000 April; 154(4):1649-1662.
[35] Gellin J, Brown S, Marshall Graves J A, et al.Comparative gene mapping workshop: progress in agriculturally important animals[J].Mamm Genome, 2000, 11(2):140-144.
[36] Miki R, Kadota. K, Bono H, et al.Delineating developmental and metabolic pathways in vivo by expression profiling using the PIKEN set of 18 186 full length enriched mouse cDNA arrays[J].Proc Natl Acad Sci USA, 2001, 98(5):2199-2204.
[37]尹俊,李金泉,张燕军,等.一个山羊I型毛角蛋白基因的序列及其在皮肤中的表达[J].畜牧兽医学报,2006, 37(1):18-22.
[38]邹丰才,黄翠琴,李明伟,等.猪蛔虫性别特异基因在第三、四期幼虫的表达谱研究[J].中国预防兽医学报,2006, 28(5):522-526.
[39] Carre W, Wang, X, Porter T E, et al.Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index[J].Physiological Genomics, 2006, 25, (5):14-524.
[40] Uenishi H, Eguchi-Ogawa T, Shinkai Hiroki, et al.PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences[J].Nucleic Acids Researc,2007, 35:D650-D653.
[41]何志颖,姚玉成.EST技术及其在基因全长cDNA克隆上的应用策略[J].国外医学遗传学分册,2002, 25(26):7-69.
[42] Gilpin BJ,Mccallum TA,Frew TJ.A linkage map of the pea(pisum stativum L) genome containing cloned sequences of known function and expressed sequence tgas(ESTs)[J].Theor Appl Genet,1997,95(8):1289-1299.
[43]苏志熙,董新妓,张兵,等.泌乳前后家猪乳腺基因表达谱的构建以及不同猪种和不同发育时期差异表达基因的确定[J].中国科学C辑,2005, 35(5):462-471.
[44]赵万里.中国鹅业发展历程及未来发展思路[J].中国禽业导刊,2000,17(9):7-9.
[45]曾凡同.养鹅全书[M].成都:四川科技出版社,1997.
[46]中国农业年鉴编委会.中国农业年鉴2002.北京:中国农业出版社,2002.
[47]陈育新,曾凡同,沈慧乐等.中国家禽.北京:农业出版社,2002.
[48] Hartman S, Touchton G, Wynn J, et al.Characterization of expressed sequence tags from a Gallus gallus pineal gland cDNA library[J].Comp Funct Genom, 2005, 6:301-306.
[49] Smith EJ, Shi L, Drummond P, et al.Expressed sequence tags for the chicken genome from a normalized 10-day-old White Leghorn whole embryo cDNA library: 1. DNA sequence characterization and linkage analysis[J].Heredity, 2001, Jan-Feb; 92(1):1-8.
[50] Smith EJ, Shi L, Prevost L, et al.Expressed sequence tags for the chicken genome from a normalized, ten-day-old white leghorn whole embryo cDNA library. 2. Comparative DNA sequence analysis of guinea fowl, quail, and turkey genomes[J].Poultry Science, 2001, Sep; 80(9):1263-1272.
[51]孔鸽萍.鹅催乳素受体基因的克隆与表达[D].南京:南京农业大学,2006.
[52]叶文凌.双峰驼下丘脑-垂体-肾上腺轴(HPAA)的功能形态[D].兰州市:兰州大学,2009.
[53]许盛海.8个鸭品种生长激素基因遗传多样性研究[D].扬州市:扬州大学动物科学与技术学院,2007.
[54]贾海燕.番鸭就巢规律及催乳素基因的SNP研究[D].福州市:福建农林大学,2007.
[55]章岩,李宁,张沅.禽类生长激素基因研究进展[J].中国畜牧杂志,1997,33(2):53-54.
[56] Riddle O, Bates R W, Lahr E L.Prolactin induces broodiness in fowl [J].Am J Physiol, 1935, 111:352-360.
[57] Bluhm C K, Phillips R E, Burkew H..Serum levels of luteinizing hormone (LH) and prolactin, estradiol and progesterone in laying and nonlaying canvasback ducks (Ay thyaalisineria) [J].Gen Comp Endocrinol, 1983, 52:1-16.
[58] Goldsmith A R, Williams D M.Incubation in mallard (Anas platy rhynchos),changes in plasma levels of prolactin and luteinizing hormone[J].J Endocrinol, 1980, 86:371-379.
[59] Hall M R, Goldsmith A R.Factors affecting prolactin secretion during breeding and incubation in the domestic duck (Anas platyrhynchos) [J].Gen Comp Endocrinol, 1983, 49:270-276.
[60]陈杰,卢立志,陈伟华,等.四季鹅繁殖周期中血浆某些生殖激素水平的变化[J].南京农业大学学报,1991,14(4):76-80.
[61] Zhu YY, Machleder EM, Chenchik A, et al.Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction[J].Biotechniques, 2001, 30(4):892-897.
[62]张阳德.生物信息学[M].北京:科学出版社,2004,103-178.
[63]王秀丽,李宁,赵兴波,等.丝毛乌骨鸡胸肌和肝脏文库的构建与鉴定[J].农业生物技术学报,2003,11(1):60-63.
[64]黄培堂.分子克隆第三版[M].北京:科学出版社,2002
[65]奥斯伯FM,金斯顿RE,赛德曼JG,等.精编分子生物学实验指南[M].第4版,马学军,舒跃龙等译,北京:科学出版社,2005 .
[66]朱靖,杨娜娜,李浩.λ噬菌体cDNA文库向质粒cDNA文库转化的研究[J].家畜生态学报,2006, 27(1):27-28.
[67] Peterson LA, Brown MR, Carlisle AJ, et al.An improved method for construction of directionally cloned cDNA libraries from microdissected cells[J].Cancer Research.1998, 58:5326-5328.
[68] Gavin AJ, Scheetz TE, Roberts CA, et al.Pooled library tissue tags for EST-based gene discovery[J].Bioinformatics2002, 18:1162-1166.
[69] OMIYA S, HIKOSO S, IMANISHI Y, et al.Downregulation of ferritin heavy chain increases labile iron pool, oxidative stress and cell death in cardiomyocytes[J].J Mol Cell Cardiol, 2009, 46:59-66.
[70] GIORDANO F J.Oxygen, oxidative stress, hypoxia, and heart failure [J].J Clin Invest.2005, 115:500-508.
[71]康波.籽鹅卵巢组织基因差异表达及产蛋相关基因定量的研究[D].长春:吉林大学畜牧兽医学院,2009.
[2]卢圣栋.现代分子生物学实验技术(第二版)[M].北京:中国协和医科大学,1999,319-328.
[3]沈倍奋.分子文库[M].北京:科学出版社,2001.
[4]黄英,张宗玉.cDNA文库构建策略[J].国外医学分子生物学分册,1999,21(6):337-340.
[5]董志敏,张宝石,关荣霞,等.全长cDNA文库的构建方法[J].中国农学通报,2006, 22(2): 51-55.
[6] Weissman SM.Molecular genetic techniques for mapping the human genome [J].Mol Biol Med, 1987, 4(3):133-143.
[7] Bonoldo MF, Lennon G, Soares MB.Normalization and Subtraction: Two approaches to facilitate gene discovery [J].Genomo Pes, 1996, 6: 791-806.
[8] Adjaye J, Daniels R, Monk.The construction of cDNA libraries from human single preimplantation embryos and their use in the study of gene expression during development [J].J Assist Reprod Genet, 1998, 15: 344-348.
[9] Vitek MP,Kreissman SG,Gross RH.The isolation of ecdysterone inducible genes by hybridization subtraction chromatography [J].Nucleic Acids Res, 1981, 9:1191-1202.
[10] Wang SM, Fears SC, Zhang L, et al.Screening poly(dAydT)+cDNAs for gene identification [J].Pro Natl Acad Sci USA, 2000, 97(8): 4162-4167.
[11] Sive HL,John TS.A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction[J].Neucleic Acid Res, 1988, 16:10937
[12] Sargent TD,Dawid IB.Differential gene expression in the Gastrula of Xenopus laevis[J].Science,1983, 222:135-139.
[13] Wang Z, Brown DD.A gene Expression Screen[J].Pro Natl Acad Sci USA, 1991, 88:15505-11509.
[14] Scbraml P,sbiPman R,Stulz P,et al.cDNA substraction library construction using a magnet-assisted substraction technique (MAST) [J].TIG, 1993, 9:70-71.
[15] Diatchenko L, Lau YFC, Campbell A P.Suppression Subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries[J].Proc Natl Sci USA, 1996, 93(12): 6025~6030.
[16] Siebert P D, Chenchik A, Kellogg D E.An improved PCR method for walking in uncloned genomic DNA[J].Nucleic Acids Res, 1995, 3:1087-1088.
[17] Sylvain Legrand, Theo Hendriks, Jean-Louis Hilbert, et al.Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L[J].BMC Plant Biology, 2007,7:27.
[18] Ho T Y,Hsiang C Y,Wu K,el al.Rapid screening of the pseudorabies virus-specific cDNAs from a cDNA library [J].J Virol Methods, 58:187-192.
[19] Thomas R.Solid phase cDNA construction,a versatile approach[J].Nucleic Acids Research, 1998, 26(14): 3451-3452.
[20]王义琴,李文彬.一种改进的cDNA文库固相构建法[J].遗传,2000, 22(4):253-254.
[21] Haas B J,Volfovsky N,Town CD,et al.Full-length messenger RNA sequences greatly improve genome annotation[J].Genome Biol,2002,3(6):RESEARCH0029.
[22] Salzberg SL,Pertea M,Delcher AL,et al.Interpolated Markov models for eukaryotic gene finding[J].Genomics,1999,59(l):24-31.
[23] Lukashin AV,Borodovsky M. GeneMark. Hmm.New solutions for gene finding [J].Nucleic Acids Res,1998,26(4):1107-1115.
[24] Burge CB,Karlin S.Prediction of complete gene structure in human genomic DNA [J].Mol Bio,1997,268:78-94.
[25] Kikuchi S,Satoh K,Nagata T,et al.Collection,mapping,and annotation of over 28,000 cDNA clones from japonica rice [J].Science,2003,301(5631):376-379.
[26] Carninci P,Kazunori Waki,Toshiyuki Shiraki,et al.Targeting a Complex Transcriptome:The Construction of the Mouse Full-Length cDNA Encyclopedia [J].Genome Res,2003,3:1273-1289.
[27] Seki M,Narusaka M,Kamiya A,et al.Functional annotation of a full-length Arabidopsis cDNA collection[J].Science,2002,296(5565) :141-145.
[28] Stapleton M,Liao GC,Carninci P,et al.The Drosophila Gene Collection: Identification of Putative Full-Length cDNAs for 70% of D. melanogaster Genes[J].GenomeRes,2002,12:1294-1300.
[29]吴伟,徐日福,高光,等.鹅肌肉组织全长cDNA文库构建及泛素Ⅰ基因序列分析[J].畜牧与兽医杂志,2007,39(5):12-15.
[30]徐日福,吴伟,高光.鹅肝脏组织的质粒cDNA文库构建.吉林农业大学学报[J].2007,29(3):325-328.
[31] Yen CF, et al.The Expression of Pituitary Gland Genes in Laying Geese[J].Poultry Science, 2006,8:2265-2269
[32] Adams M D, Kelley J M, Gocayne J D, et al.Complementary DNA Sequencing: Sequence Tags and Human Genome Project [J].Science, 1991, 252:1651-1656.
[33] Harushima Y, Yano M, Shomura A, et al.A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics [J].1998 January; 148(1):479-494.
[34] Brent A E, MacQueen A, Hazelrigg T.The Drosophila wispy gene is required for RNA localization and other microtubule-based events of meiosis and early embryogenesis [J].Genetics. 2000 April; 154(4):1649-1662.
[35] Gellin J, Brown S, Marshall Graves J A, et al.Comparative gene mapping workshop: progress in agriculturally important animals[J].Mamm Genome, 2000, 11(2):140-144.
[36] Miki R, Kadota. K, Bono H, et al.Delineating developmental and metabolic pathways in vivo by expression profiling using the PIKEN set of 18 186 full length enriched mouse cDNA arrays[J].Proc Natl Acad Sci USA, 2001, 98(5):2199-2204.
[37]尹俊,李金泉,张燕军,等.一个山羊I型毛角蛋白基因的序列及其在皮肤中的表达[J].畜牧兽医学报,2006, 37(1):18-22.
[38]邹丰才,黄翠琴,李明伟,等.猪蛔虫性别特异基因在第三、四期幼虫的表达谱研究[J].中国预防兽医学报,2006, 28(5):522-526.
[39] Carre W, Wang, X, Porter T E, et al.Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index[J].Physiological Genomics, 2006, 25, (5):14-524.
[40] Uenishi H, Eguchi-Ogawa T, Shinkai Hiroki, et al.PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences[J].Nucleic Acids Researc,2007, 35:D650-D653.
[41]何志颖,姚玉成.EST技术及其在基因全长cDNA克隆上的应用策略[J].国外医学遗传学分册,2002, 25(26):7-69.
[42] Gilpin BJ,Mccallum TA,Frew TJ.A linkage map of the pea(pisum stativum L) genome containing cloned sequences of known function and expressed sequence tgas(ESTs)[J].Theor Appl Genet,1997,95(8):1289-1299.
[43]苏志熙,董新妓,张兵,等.泌乳前后家猪乳腺基因表达谱的构建以及不同猪种和不同发育时期差异表达基因的确定[J].中国科学C辑,2005, 35(5):462-471.
[44]赵万里.中国鹅业发展历程及未来发展思路[J].中国禽业导刊,2000,17(9):7-9.
[45]曾凡同.养鹅全书[M].成都:四川科技出版社,1997.
[46]中国农业年鉴编委会.中国农业年鉴2002.北京:中国农业出版社,2002.
[47]陈育新,曾凡同,沈慧乐等.中国家禽.北京:农业出版社,2002.
[48] Hartman S, Touchton G, Wynn J, et al.Characterization of expressed sequence tags from a Gallus gallus pineal gland cDNA library[J].Comp Funct Genom, 2005, 6:301-306.
[49] Smith EJ, Shi L, Drummond P, et al.Expressed sequence tags for the chicken genome from a normalized 10-day-old White Leghorn whole embryo cDNA library: 1. DNA sequence characterization and linkage analysis[J].Heredity, 2001, Jan-Feb; 92(1):1-8.
[50] Smith EJ, Shi L, Prevost L, et al.Expressed sequence tags for the chicken genome from a normalized, ten-day-old white leghorn whole embryo cDNA library. 2. Comparative DNA sequence analysis of guinea fowl, quail, and turkey genomes[J].Poultry Science, 2001, Sep; 80(9):1263-1272.
[51]孔鸽萍.鹅催乳素受体基因的克隆与表达[D].南京:南京农业大学,2006.
[52]叶文凌.双峰驼下丘脑-垂体-肾上腺轴(HPAA)的功能形态[D].兰州市:兰州大学,2009.
[53]许盛海.8个鸭品种生长激素基因遗传多样性研究[D].扬州市:扬州大学动物科学与技术学院,2007.
[54]贾海燕.番鸭就巢规律及催乳素基因的SNP研究[D].福州市:福建农林大学,2007.
[55]章岩,李宁,张沅.禽类生长激素基因研究进展[J].中国畜牧杂志,1997,33(2):53-54.
[56] Riddle O, Bates R W, Lahr E L.Prolactin induces broodiness in fowl [J].Am J Physiol, 1935, 111:352-360.
[57] Bluhm C K, Phillips R E, Burkew H..Serum levels of luteinizing hormone (LH) and prolactin, estradiol and progesterone in laying and nonlaying canvasback ducks (Ay thyaalisineria) [J].Gen Comp Endocrinol, 1983, 52:1-16.
[58] Goldsmith A R, Williams D M.Incubation in mallard (Anas platy rhynchos),changes in plasma levels of prolactin and luteinizing hormone[J].J Endocrinol, 1980, 86:371-379.
[59] Hall M R, Goldsmith A R.Factors affecting prolactin secretion during breeding and incubation in the domestic duck (Anas platyrhynchos) [J].Gen Comp Endocrinol, 1983, 49:270-276.
[60]陈杰,卢立志,陈伟华,等.四季鹅繁殖周期中血浆某些生殖激素水平的变化[J].南京农业大学学报,1991,14(4):76-80.
[61] Zhu YY, Machleder EM, Chenchik A, et al.Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction[J].Biotechniques, 2001, 30(4):892-897.
[62]张阳德.生物信息学[M].北京:科学出版社,2004,103-178.
[63]王秀丽,李宁,赵兴波,等.丝毛乌骨鸡胸肌和肝脏文库的构建与鉴定[J].农业生物技术学报,2003,11(1):60-63.
[64]黄培堂.分子克隆第三版[M].北京:科学出版社,2002
[65]奥斯伯FM,金斯顿RE,赛德曼JG,等.精编分子生物学实验指南[M].第4版,马学军,舒跃龙等译,北京:科学出版社,2005 .
[66]朱靖,杨娜娜,李浩.λ噬菌体cDNA文库向质粒cDNA文库转化的研究[J].家畜生态学报,2006, 27(1):27-28.
[67] Peterson LA, Brown MR, Carlisle AJ, et al.An improved method for construction of directionally cloned cDNA libraries from microdissected cells[J].Cancer Research.1998, 58:5326-5328.
[68] Gavin AJ, Scheetz TE, Roberts CA, et al.Pooled library tissue tags for EST-based gene discovery[J].Bioinformatics2002, 18:1162-1166.
[69] OMIYA S, HIKOSO S, IMANISHI Y, et al.Downregulation of ferritin heavy chain increases labile iron pool, oxidative stress and cell death in cardiomyocytes[J].J Mol Cell Cardiol, 2009, 46:59-66.
[70] GIORDANO F J.Oxygen, oxidative stress, hypoxia, and heart failure [J].J Clin Invest.2005, 115:500-508.
[71]康波.籽鹅卵巢组织基因差异表达及产蛋相关基因定量的研究[D].长春:吉林大学畜牧兽医学院,2009.