与辣（甜）椒抗根结线虫Me1基因紧密连锁的EST-SSR分子标记的开发
摘要
根结线虫是辣(甜)椒的主要病害之一,其危害日趋严重。培育抗性品种是防治根结线虫最有效的方法,常规育种周期长、消耗大,利用分子标记辅助育种则会大大提高育种速度。EST-SSR作为一种新型分子标记,来自表达基因,除具备传统基因组来源的SSR标记所有优势外,还可能与基因功能具有直接或间接关系,从而强化了SSR标记在遗传研究中的应用。
本研究以辣椒EST数据为基础,通过分析辣椒EST-SSR位点信息,建立了部分辣椒EST-SSR标记,并开发与辣椒抗根结线虫显性单基因Mel紧密连锁的分子标记,为Me1基因的标记辅助选择奠定基础。主要研究结果如下：
1.利用SSRIT软件(http://www.gramene.org/gremene/searches/ssrtool)从NCBI(http://www.ncbi.nlm.nih.gov/dbEST)数据库中19173条辣椒EST序列中筛选到612个SSR,检出率为3.19%。共观察到51种重复基元,其中三核苷酸基序是主导类型,占总SSR的60.46%；AG/GA/CT/TC, AAG/AGA/GAA/CTT/TTC/TCT, TAAT, ATATA和CTGCTC分别是二、三、四、五和六核苷酸基序的优势重复基序,在所有SSR中的出现频率各自为13.40%、16.18%、1.80%、3.27%及2.12%。辣椒EST-SSR以5-10次重复为主,基序长度集中在15-20bp范围内。
2.利用筛选出含SSR的576条EST序列,设计了357对引物,在合适的PCR条件下,利用非变性聚丙烯酰胺凝胶银染检测这些引物在6个辣(甜)椒品种中的扩增情况和多态性。其中有337对引物显示出清晰条带,引物有效扩增率为94.40%；75对引物表现多态性,占有效扩增引物的21.01%。
3.用含抗根结线虫病基因Me1的PM217与典型感线虫品种茄门,及其F1、F2代为试材,采用南方根结线虫(Meloidogyne spp.)人工接种鉴定,每株接种2000卵,两个月后进行抗病性鉴定,根据鉴定结果,利用分离群体分组分析法(bulked segregant analysis, BSA)建立抗感池,共筛选到4对(118、141、142及211)多态性EST-SSR引物在抗感池间存在差异,分别将该4个标记位点暂命名为118、141、142及211。
4.利用Joinmap3.0软件,结合抗病性鉴定,对271个单株的F2群体进行SSR分析,研究4个EST-SSR标记位点与根结线虫抗性基因Me1的连锁关系,结果表明,EST-SSR标记141、118及211与Me1连锁程度较高,遗传距离分别为6.984 cM、18.684cM和29.310cM。
Root-knot nematode is one of the main disease of pepper, having brought increasingly serious harm. The most effective way to control this disease is breeding resistant pepper. Traditional breeding has the disadvantage of long cycle and high cost, and molecular mark assisted breeding will greatly improve efficiency of breeding. EST-SSR is a new type of molecular marker developed from the databank of sequence of expression sequence tags (EST) and cDNAs. As a new kind of molecular marker, EST-SSR markers have more advantages than the traditional genomic-derived SSRs because they are part of expressed genes. Therefore, EST-SSRs might be involved in gene functioning directly or indirectly. The ability of SSR markers will be greatly enhanced.
In this study, we analyzed SSR resource of ESTs data in Capsicum, and preliminary developed soMe EST-SSR markers, and identified molecular markers linked to the dominant resistant gene Me1 laying the foundation for resistance breeding. The main results were following.
1. In this report,19173 ESTs of Capsicum annuum were downloaded from NCBI, from which 612 SSRs were screened, by using software SSRIT, and the frequency was 3.19%.51 types of repeat motifs were detected and the dominant type was trinucleotide which occupied 60.46% out of total SSRs. Predominant motifs of di-, tri-, tetra-, penta-and hexa-nucleotide repeats were AG/GA/CT/TC, AAG/AGA/GAA/CTT/TTC/TCT, TAAT, ATATA and CTGCTC, with the frequencies of 13.40%,16.18%,1.80%,3.27% and 2.12% respectively. The repeat number of pepper EST-SSR was mainly 5-10 and the motif length ranged from 15bp to 20bp.
2. We designed 357 candidate priMer pairs for 576 EST-SSRs. Under optimal PCR conditions, The amplification and polymorphism displayed by these priMers in 6 varieties of pepper were detected by using silver staining of non-denaturing polyacrylamide gel.337 priMer pairs (94.40%) showed clear amplification, and 75 of them (21.01%) demonstrated polymorphism.
3. The materials were F1 and a F2 population from PM217 with the resistant gent Me1, and the typical susceptible variety QieMen. Using artificial inoculation,2000 eggs of southern root-knot nematodes per plant were poured to investigate the number of root knots after inoculated for two months. Base on the results of the investigation, a resistance group and a sensitive group were divided with 'BSA' Methods to search for the EST-SSR polymorphic priMer combinations priMer 118,141,142 and 211, and tentatively naMed EST-SSR markers 118,141,142 and 211.
4. By the software JoinMap V3.0, the relationship of 4 EST-SSR molecular markers and resistant segregation of F2 population were analyzed.The results indicated that the distance between the EST-SSR molecular markers141,118 and 211 and the nematodes resistance Me1 gene in pepper were 6.984cM,18.684cM and 29.310cM respectively.
本研究以辣椒EST数据为基础,通过分析辣椒EST-SSR位点信息,建立了部分辣椒EST-SSR标记,并开发与辣椒抗根结线虫显性单基因Mel紧密连锁的分子标记,为Me1基因的标记辅助选择奠定基础。主要研究结果如下：
1.利用SSRIT软件(http://www.gramene.org/gremene/searches/ssrtool)从NCBI(http://www.ncbi.nlm.nih.gov/dbEST)数据库中19173条辣椒EST序列中筛选到612个SSR,检出率为3.19%。共观察到51种重复基元,其中三核苷酸基序是主导类型,占总SSR的60.46%；AG/GA/CT/TC, AAG/AGA/GAA/CTT/TTC/TCT, TAAT, ATATA和CTGCTC分别是二、三、四、五和六核苷酸基序的优势重复基序,在所有SSR中的出现频率各自为13.40%、16.18%、1.80%、3.27%及2.12%。辣椒EST-SSR以5-10次重复为主,基序长度集中在15-20bp范围内。
2.利用筛选出含SSR的576条EST序列,设计了357对引物,在合适的PCR条件下,利用非变性聚丙烯酰胺凝胶银染检测这些引物在6个辣(甜)椒品种中的扩增情况和多态性。其中有337对引物显示出清晰条带,引物有效扩增率为94.40%；75对引物表现多态性,占有效扩增引物的21.01%。
3.用含抗根结线虫病基因Me1的PM217与典型感线虫品种茄门,及其F1、F2代为试材,采用南方根结线虫(Meloidogyne spp.)人工接种鉴定,每株接种2000卵,两个月后进行抗病性鉴定,根据鉴定结果,利用分离群体分组分析法(bulked segregant analysis, BSA)建立抗感池,共筛选到4对(118、141、142及211)多态性EST-SSR引物在抗感池间存在差异,分别将该4个标记位点暂命名为118、141、142及211。
4.利用Joinmap3.0软件,结合抗病性鉴定,对271个单株的F2群体进行SSR分析,研究4个EST-SSR标记位点与根结线虫抗性基因Me1的连锁关系,结果表明,EST-SSR标记141、118及211与Me1连锁程度较高,遗传距离分别为6.984 cM、18.684cM和29.310cM。
Root-knot nematode is one of the main disease of pepper, having brought increasingly serious harm. The most effective way to control this disease is breeding resistant pepper. Traditional breeding has the disadvantage of long cycle and high cost, and molecular mark assisted breeding will greatly improve efficiency of breeding. EST-SSR is a new type of molecular marker developed from the databank of sequence of expression sequence tags (EST) and cDNAs. As a new kind of molecular marker, EST-SSR markers have more advantages than the traditional genomic-derived SSRs because they are part of expressed genes. Therefore, EST-SSRs might be involved in gene functioning directly or indirectly. The ability of SSR markers will be greatly enhanced.
In this study, we analyzed SSR resource of ESTs data in Capsicum, and preliminary developed soMe EST-SSR markers, and identified molecular markers linked to the dominant resistant gene Me1 laying the foundation for resistance breeding. The main results were following.
1. In this report,19173 ESTs of Capsicum annuum were downloaded from NCBI, from which 612 SSRs were screened, by using software SSRIT, and the frequency was 3.19%.51 types of repeat motifs were detected and the dominant type was trinucleotide which occupied 60.46% out of total SSRs. Predominant motifs of di-, tri-, tetra-, penta-and hexa-nucleotide repeats were AG/GA/CT/TC, AAG/AGA/GAA/CTT/TTC/TCT, TAAT, ATATA and CTGCTC, with the frequencies of 13.40%,16.18%,1.80%,3.27% and 2.12% respectively. The repeat number of pepper EST-SSR was mainly 5-10 and the motif length ranged from 15bp to 20bp.
2. We designed 357 candidate priMer pairs for 576 EST-SSRs. Under optimal PCR conditions, The amplification and polymorphism displayed by these priMers in 6 varieties of pepper were detected by using silver staining of non-denaturing polyacrylamide gel.337 priMer pairs (94.40%) showed clear amplification, and 75 of them (21.01%) demonstrated polymorphism.
3. The materials were F1 and a F2 population from PM217 with the resistant gent Me1, and the typical susceptible variety QieMen. Using artificial inoculation,2000 eggs of southern root-knot nematodes per plant were poured to investigate the number of root knots after inoculated for two months. Base on the results of the investigation, a resistance group and a sensitive group were divided with 'BSA' Methods to search for the EST-SSR polymorphic priMer combinations priMer 118,141,142 and 211, and tentatively naMed EST-SSR markers 118,141,142 and 211.
4. By the software JoinMap V3.0, the relationship of 4 EST-SSR molecular markers and resistant segregation of F2 population were analyzed.The results indicated that the distance between the EST-SSR molecular markers141,118 and 211 and the nematodes resistance Me1 gene in pepper were 6.984cM,18.684cM and 29.310cM respectively.
引文
[1]北京农业大学编.蔬菜栽培学(保护地栽培)[M].北京：农业出版社,1993：259-264
[2]中华人民共和国农业部主编.中国农业统计资料.北京：中国农业出版社,2003：318
[3]泰勒A L,萨塞J N.植物根结线虫[M].北京：科学出版社,1983：3.
[4]Abad P, Favery B, Rosso M N. Root-knot nematode parasitism and host response:molecular basis of a sophisticated interaction[J]. Molecular plant pathology,2003,4(4):217-224
[5]张芸,胡铁军,师迎春.北京地区蔬菜根结线虫病发生特点及控制对策[J].中国农技推广,2005(2)：43-45
[6]Karssen Gerrit. The Plant-parasitic Nematode Genus Meloidogyne Goldi,1892 (Tylenchida) in Europe[J]. [PhD Thesis]. The Netherlands, Gent Belgium:Gent University,1999
[7]武扬,郑经武,商晗武等.根结线虫分类和鉴定途径及进展[J].浙江农业学报,2005,17(2)：106-110。
[8]孙运达,王献杰,蔬菜地根结线虫病的发生特点及综防技术[J],中国蔬菜,1996,6：36-37
[9]符美英,陈绵才,肖彤斌等.根结线虫与寄主植物互作机理的研究进展[J].热带农业科学,2008,28(3)：73-74
[10]孙世伟,桑利伟.根结线虫防治研究进展[J].现代农业科技,2008,11：181-182
[11]张芸,胡铁军,师迎春.蔬菜根结线虫病药剂防治的实践体会[J]中国植保导刊,2004,24(10)：18-19
[12]孔凡彬,谢国红.茄子根结线虫发生规律及综合防治技术[J].安徽农业科学,2005,33(3)：446.
[13]蒋淑芝,张志斌,张真和.灌注热水消毒法防治设施蔬菜土传病虫害技术考察[J].中国蔬菜,2005,9：41
[14]房华,廖金铃,吴庆丽等.茶树菇菌渣对番茄根结线虫病防治的初步研究[J].莱阳农学院学报,2004,21(2)：182-184
[15]吴家琴,陈绵才,张怀芳.红麻生长期病虫害防治研究[J].中国麻作,1995,17(2)：25-28.
[16]马爱瑛,张靠稳,马岩根结线虫的生物防治[J].河北农业科学,200,12(2)：60-62
[17]崔林开,胡艳红,郭岩蔬菜根结线虫生物防治的研究进展[J].安徽农业科学,2006,34(22)：5907-5908
[18]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京：科学出版社,2002,1-20
[19]Botstein D.et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am.J.Hum.Genet,1980,32:314-316
[20]Williams J G, Kubelik A R, Livak K J. et al. DNA polymorphism amplified by arbitrary priMers are useful as genetic markers [J].Nucleic Acids Res,1990,18:6531-6535
[21]Welsh J, Meelelland M. Fingerprinting genomes using PCR with arbitray Primers [J]. Nueleie Aeids Res,1990,18:7213-7219
[22]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polyMerase chain reaction amplification[J].Genomics,1994,20:176-183
[23]Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers[J]. Nucleic Acids Research,1989,17:6463-6471
[24]Litt M, Luty J A. A hypervariable. Microsatellite revealed by in vitro amplification of dinucleotide repeat within the cardiac muscle actin gene[J].American Journal of Human Genetics,1989,44:397-401
[25]Zabeau M,Vos P.Selective restriction fragment amplification:a general Method for DNA fingerprinting,French,EP0534858[P].1992-09-24.
[26]Vos P,Hogers R,Bleeker M et al.A new technique for DNA fingerprinting[J].Nucleic Acids Research,1995,23(21):4407-4414.
[27]Konieczny A, Ausubel F M. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers[J]. Plant Journal,1993,4:403-410
[28]郝岗平,杨清,吴忠义等.植物的单核苷酸多态性及其在作物遗传育种中的应用[J].植物学通报,2004,21(5)：618-624
[29]Adams MD, Kelley JM, Gocayne JD et al. Complementary DNA Sequencing:Expressed Sequence Tags and Human GenoMe Project[J]. Science,1991,252:1651-1656,
[30]忻雅,崔海瑞,植物表达序列标签(EST)标记及其应用研究进展[J].生物学通报,2004,39(8)：4-6
[31]于凤池.EST技术及其应用综述[J].农业生物技术科学,2005,21(2)：54-58
[32]李小白,崔海瑞,张明龙等.EST分子标记开发及在比较基因组学中的应用[J].生物多样性,2006,14(6)：541-547
[33]Cordeiro GM, Casu R, McIntyre CL et al. Microsatellite markers from sugarcane(Saccharum spp.) ESTs cross transferable to Erianthus and Sorghum[J].Plant Science,2001,160:1115-1123
[34]Areshehenkova T, Ganal MW. Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources[J]. Theoretical and Applied Genetics,2002,104:229-235
[35]Scott KD, Eggler P, Seaton P et al. Analysis of SSRs derived from grape ESTs[J].Theor Appl Genet.2000,100:723-726,
[36]Eujayl I, Sorrells M, Baum M et al.Powell W Assessment of genotypic variation among cultivated durum wheat based on EST-SSR and genomic SSRs[J]. Euphytica,2001,119:39-43.
[37]Hackauf B.Wehling P. Identification of microsatellite polymorphisms in an expressed portion of the rye genoMe[J]. Plant Breed,2002,121:17-25
[38]Varshney RK, Sigmund R, Borner A et al. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice[J]. Plant Science,2005,168,195-202
[39]Song Q J,Marek L F,Shoemaker RC et al. A new integrated genetic linkage map of t he soybean[J]. Theor Appl Genet,2004,109:122-128
[40]Kantety R V, Rota M L, Matthews D E.et al. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat[J]. Plant Molecular Biology,2002, 48:501-510
[41]Cherdsak Liewlak saneeyanawin, Carol E Ritland, Yousry AEl-Kassaby et al. Single-copy, species-transferable micro satellite markers developed from loblolly pine ESTs[J].Theor Appl Genet,2004,109:361-369
[42]Malay C Saha,M A Rouf Mian,Imad Eujayl et al. Tall fescue EST-SSR markers with transferability across several grass species[J].Theor Appl Genet,2004,109:783-791
[43]Huang S.W., Zhang B.X., Milbourne D et al. Development of pepper SSR marker from sequence database [J], Euphytica,2000,117(2):163-167
[44]李晶晶,王述彬,刘金兵等.辣椒EST-SSR标记的开发[J].分子植物育种.2008,6(6)：1219-1222
[45]谢皓,陈学珍,杨柳等.EST-SSR标记的发展和在植物遗传研究中的应用[J].北京农学院学报,2005,20(4)：73-76
[46]邹礼平.分子标记在茄科蔬菜作物遗传育种研究中的应用[J].孝感学院学报(自然科学版),2000,20(4)：78-81
[47]黄三文，王晓武,张宝玺等.蔬菜作物分子标记研究进展与我国的发展策略[J].中国蔬菜,1999,1：50-53
[48]Wang Jauyueh. Study on the molecular markers of Capsicum wild domesticated species using RAPD analysis[J]. Journal of Agricultural Research of China,1966,45 (4):370-381
[49]Fan-Mingjen. Analysis of the genetic diversity of Capsicum ssp.(pepper) using RAPD analysis[J]. Journal of Agricultural Research of China,2001,50 (4):29-42
[50]JP Prince, VK Lackney,C Angeles et al. A survey of DNA polymorphism within the genus Capsicum and the fingerprinting of pepper cultivars [J]. Genome,1995,38 (2):224-231
[51]T Pradeepkumar,J L Karihaloo,Sunil Archak. Molecular characterization of Piper nigrum L.cultivars using RAPD markers[J]. Current Science,2001,81 (3):246-248
[52]I Paran, E Aftergoot,C Shifriss. Variation in Capsicum annuum revealed by RAPD and AFLP markers[J]. Euphytica,1998,99 (3):167-173
[53]V Lefebvre,B Goffinet,JC Chauvet et al. Evaluation of genetic distances between pepper inbred lines for cultivar protection purposes:comparison of AFLP, RAPD and Phenotypic data[J]. Theoretical and Applied Genetics,2001,102 (5):741-750
[54]J P Prince,E Pochard,SD Tanksley et al. Construction of a molecular linkage map of pepper and a comparison of synteny with tomato [J].Genome.1993,36(3):404-407
[55]A Ben chain, I Paran,RC Grube et al. QTL mapping of fruit related traits in pepper[J]. Theoretical and Applied Genetics,2001,102(6-7):1016-1028
[56]V Lefebvre,A Palloix,C Caranta et al. Construction of an intraspecific integrated map of pepper using molecular markers and doubled haploid progenies [J]. Genome,1995,38(1):112-121
[57]Yasuhiro Minamiyama,Masato Tsuro (?),Masashi Hirai. An SSR-based linkage map of Capsicum annuum [J].Mol Breeding,2006,18:157-169
[58]Han Paran, Jeroen Rouppe van der Voort et al. An integrated genetic linkage map of pepper (Capsicum spp.) [J], Molecular Breeding,2004,13:251-261
[59]Caranta C. Development of a CAPS marker for the Pvr4 locus a tool for pyramiding polyvirus resistance genes in pepper[J].Genome,1999,42(6):1111-1116
[60]Tai-T. High resolution genetic and physical mapping of the region containing the Bs2 resistance gene of pepper[J]. Theoretical and Applied Genetics,1999,99(7-8):1201-1206
[61]Pierre M. High resolution genetic mapping of the pepper resistance locus Bs3 governing recognition of the Xanthomonas Campestirs pv vesicatoria Avr Bs3 protein[J]. Theoretical and Applied Genetics,2000,101(1-2):255-263
[62]Choe Youngwon.Pepper (Capsicum annuum L) cultivar identification and seed purity test by RAPD and PGM isozyme RDA[J]. Journal of Horticulture Science,1998,40(1):15-23
[63]马艳青.分子标记技术及其在辣椒遗传育种上的应用[J].辣椒杂志(季刊),2004,3：1-8
[64]Moury B A.CAPS marker to assist selection of tomato spotted with virus resistance in pepper[J].Genome.2000,43(1):137-142
[65]Z Baoxi,H Sanwen,Y GuImei et al. Two RAPD markers linked to a major fetility restorer gene in pepper[J].Euphytica,2000,113(2):155-161
[66]MartinJA. Breeding of pungent peppers[J].South Carolina Agr. Expt.Sta.Annu.Rpt,1948,60:64-67
[67]Hare W W. Inheritance of resistance to root-knot nematodes in pepper [J].Phytopathology,1957,47: 455-459
[68]Martin J A, Crawford J H. Carolina Hot pepper[J]. South Carolina Agr. Expt. Sta.Cir.,1958:117
[69]黄三文,张宝玺,郭家珍等.辣(甜)椒根结线虫的危害、防治和抗病育种[J].园艺学报,2000,27(增刊)：515-521
[70]Hendy H, Pochard E, Dalmasso A. Transmission hereditaire de laresistance aux nematodes Meloidogyne Chitwood (Tylenchida) portee par 2 lignees de Capsicum annuum L:etude de descendances homozygotesissues d'androgenese[J].Agronomic,1985,5(2):93-100
[71]Bleve-Zacheo T, Bongiovanni M, Melillo M T et al. The pepper resistance genes Me1 and Me3 induce differential penetration rates and temporal sequences of root cellultra structural changes upon nematode infection[J]. Plant Science,1998,133:79-90
[72]C. Djian-Caporalino, A. Fazari,M. J. Arguel.et.al. Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome[J].Theor Appl Genet,2007,114:473-486
[73]Di Vito M,Zaccheo G,Catalano F.Effect of temperature on stability of resistance to root—knot nematode(Metoidogyne spp.)Ⅸth EUCARPIA meeting on Genetics and Breeding of Capsicum & Eggplant[J],Budapest,Hungary,1995,230-232
[74]顾晓慧,王立浩,毛胜利等.辣椒根结线虫防治与抗性育种研究进展[J].中国蔬菜,2006,5：33-36
[75]Vito M D,Saccardo F,Zaccheo G. Response of New Lines of Pepper to Meloidogyne incognita, M. javanica,M. arenaria and M. hapla[J]. Afro-Asian Journal of Nematology,1993,3(2):135-138
[76]Fery R L,Dukes P D Sr,Thies J A. Carolina Wonder and Charleston Belle:southern root-knot nematode-resistant bell peppers[J]. Hort-Science,1998,33(5):900-902
[77]C Djian-Caporalino-C,L Pijarowski,A Fazari et al.High-resolution genetic mapping of the pepper (Capsicum annuum L) resistance loci Me3 and Me4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.) [J]. Theor Appl Genet,2001,103:592-600
[78]顾晓慧.与辣椒抗根结线虫N基因紧密连锁的AFLP标记的开发[D].2006
[79]刘维志.病原植物线虫学[M].中国农业出版社,2000：372-425
[80]C Djian-Caporalino C, Pijarowski L, Januel A et al. Spectrum of resistance to root-knot nematodes and inheritance of heatstable resistance in pepper(Capsicum annuum L) [J].Theor Appl Genet, 1999,99:496-502
[81]陈海梅,李林志,卫宪云等.小麦EST-SSR标记的开发、染色体定位和遗传作图[J],科学通报,2005,50(20)：2208-2216
[82]SM Tam,C Mhiri,A Vogelaar et al. Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR[J].Theor Appl Genet,2005,110:819-831
[83]毛爱军,柴敏,于拴仓等.番茄抗根结线虫接种鉴定技术及其应用[J]西北农业学报.2005,14(4)：140-144
[84]Eujayl I, Sorrells ME, Baum M et al. Isolation of EST-derived microsatellite markers for genotyping the A and B genoMes of wheat[J]. Theor Appl Genet.2002,104:399-407
[85]Barrett B, Griffiths A, Schreiber M et al.A microsatellite map of white clover[J]. Theor Appl Genet, 2004,109:596-608
[86]Varshney R.K., Sigmund R., Korzun V et al Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice[J]. Plant Science,2005,168(1):195-202
[87]Han Z G, Guo W Z, Song X L et al.Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton[J]. Mol Genet Genomics,2004,272:308-327
[88]Gibum Yi, Je Min Lee, Sanghyeob Lee et al. Exploitation of pepper EST-SSRs and an SSR-based linkage map[J].Theor Appl Genet.2006,114:113-130
[89]胡勇胜,毛胜利,王立浩等.辣椒SSR反应体系优化[J],辣椒杂志,2007(2)：36-40
[90]Gupta P K,Rustgi S,Sharma S et al.Transferable EST-SSRs markers for the study of polymorphism and genetic diversity in bread wheat[J]. Mol.Gen.Genomics.2003,270 (4):315-323
[91]B. Jay a shreea, Ramu Punnab, P. Prasada.A Database of Simple Sequence Repeats from Cereal and Legume Expressed Sequence Tags Mined in silico:Survey and Evaluation In Silico[J] Bfology.2006,6:607-62
[92]Siva P.Kumpatla, Snehasis Mukhopadhyay. Mining and survey of simple sequence repeats in expressed sequence tags of dicotyledonous species [J]. Genome.2005,48:985-998
[93]Portis E., Nagy I., Sasvari Z et al. The design of Capsicum spp. SSR assays via analysis of in silico DNA sequence, and their potential utility for genetic mapping [J]. Plant Science.2007,172 (3): 640-648
[94]Mukund V. Katti, Prabhakar K. Ranjekar et al. Differential Distribution of Simple Sequence Repeats in Eukaryotic Genome Sequences[J]. Mol. Biol. Evol.2001,18 (7):1161-1167
[95]Jerzy Jurka, Charit Pethiyagoda. Simple repetitive DNA sequences from primates:Compilation and analysis [J]. Mol Evol,1995,40:120-126
[96]Linda Cardle, Luke Ramsay, Dan Milbourne et al. Computational and ExperiMental Characterization of Physically Clustered Simple Sequence Repeats in Plants[J]. Genetics.2000,156: 847-854
[97]Tsukasa Nunome, Satomi Negoro, Izumi Kono. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.)[J].Theor Appl Genet.2009, 119:1143-1153
[98]Jung Sook.Albert Abbott.Christopher Jesudurai.Frequency, type, distribution and annotation of simple sequence repeats in Rosaceae ESTs Funct Integr[J].Genomics.2005,5:136-143
[99]张俊娥,李芬,孙蒙祥烟草EST-SSR位点分析[J].武汉植物学研究.2007,25(5)：427-431
[100]Morgante M, Hanafey M, Powell W. Microsatellites are preferentially associated with nonrepeatitive DNA in plant genomes[J]. Nat.Genet.2002,30:194-200
[101]Mauricio La Rota, RaMesh V,Kantety Ju-Kyung Yuet al. Nonrandom distribution and frequencies of genomic and EST-derived microsatellite markers in rice, wheat, and barley[J]. BMC Genomics 2005,6:23
[102]B. Jayashreea,, Ramu Punnab, P. Prasada et al. A Database of simple sequence repeats from cereal and legume expressed sequence tags mined in silico survey and evaluation. In Silico[J]. Biology.2006,6:607-620
[103]N. Nicot,.V. Chiquet, B. Gandon.Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs)[J], Theor Appl Genet.2004,109:800-805
[104]T. Areshchenkova,M. W. Ganal. Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources[J]. Theor ApplGenet.2002,104：229-235
[105]Yinglei Lai, Fengzhu Sun.The Relationship between microsatellite slippage mutation rate and the number of repeat units[J]. Molecular Biology and Evolution.2003,20(12):2123-2131
[106]Xu X, PengM,Fang Z et al. The direction of microsatellite mutations is dependent upon allele length[J]. Nat Genet.2000,24:396-399
[107]骆蒙,贾继增.植物基因组表达序列标签(EST)计划研究进展[J].生物化学与生物物理进展,2001,28(4)：494-497
[108]C.Djian Caporalino, L.Pijarowski,A.Januel et al.Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome [J]. Theor Appl Genet (2007) 114:473-486
[109]Thies, J. A.,J. D. Mueller et al.Use of a resistant pepper as a rotational crop to manage southern root-knot nematode[J]. HortScience,1998,33:716-718.
[110]Thies J. A., Fery, R. L. Characterization of resistance conferred by the N gene to Meloidogyne arenaria races 1 and 2, M. hapla, and M. javanica in two sets of isogenic lines of Capsicum annuum L[J]. Journal of the American Society for Horticultural Science,2000,125,71-75
[111]Michelmore R.W, Paran, Kesseli R.V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:A rapid Method to detect markers in specific genomic regions by using segregating populations(random amplified polymorphic DNA/restriction fragMent length polymorphism)[J].Genetics Proc. Natl. Acad. Sci.1991,88(11):9828-9832,
[112]Fausto de Souza-Sobrinho, Wilson Roberto Maluf, Luiz A.A.et al.Inheritance of resistance to Meloidogyne incognita race 2 in the hot pepper cultivar Carolina Cayenne(Capsicum annuum L.)[J].Genetics and Molecular Research,2002,3:271-279
[113]茆振川,谢丙炎,杨宇红等.辣椒N基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析[J].园艺学报,2007,34(3)：629-636
[114]茆振川,谢丙炎,杨宇红等.辣椒Me3基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析[J].园艺学报,2007,34(6)：1453-1458
[115]Rugang Chen, Hanxia Li,Liying Zhang et al. CaMi, a root-knot nematode resistance gene from hot pepper (Capsium annuum L.) confers nematode resistance in tomato[J]. Plant Cell Rep,2007, 26:895-905
[116]Judy A. Thies. Jennifer J. Ariss. Comparison between the N and Me3 genes conferringresistance to the root-knot nematodes(Melodiogyne incognita) in genetically different pepper lines(Capsicum annuum)[J].Eur J Plant Pathol,2009,8:doi 10.1007/s10658-00909502-7
[117]L.H. Wang, X.H. Gu, M.Y. Hua et al.A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)[J].Scientia Horticulturae,2009(122),2:318-322
[118]A. Piedra Buena,J.A.Lopez-Perez, A. Bello et al.Screening of cuarentino pepper(capsicum annuum) for resistance to Meloidogyne incognita[J]. Nematropica,2006,36(1):13-22
[119]茆振川.辣椒与根结线虫不亲和互作相关基因的分离及功能分析[D].2007
[120]王添成.蔬菜抗病筛选技术[A].台湾省农业试验所：蔬菜育种技术研习会专刊,1998
[2]中华人民共和国农业部主编.中国农业统计资料.北京：中国农业出版社,2003：318
[3]泰勒A L,萨塞J N.植物根结线虫[M].北京：科学出版社,1983：3.
[4]Abad P, Favery B, Rosso M N. Root-knot nematode parasitism and host response:molecular basis of a sophisticated interaction[J]. Molecular plant pathology,2003,4(4):217-224
[5]张芸,胡铁军,师迎春.北京地区蔬菜根结线虫病发生特点及控制对策[J].中国农技推广,2005(2)：43-45
[6]Karssen Gerrit. The Plant-parasitic Nematode Genus Meloidogyne Goldi,1892 (Tylenchida) in Europe[J]. [PhD Thesis]. The Netherlands, Gent Belgium:Gent University,1999
[7]武扬,郑经武,商晗武等.根结线虫分类和鉴定途径及进展[J].浙江农业学报,2005,17(2)：106-110。
[8]孙运达,王献杰,蔬菜地根结线虫病的发生特点及综防技术[J],中国蔬菜,1996,6：36-37
[9]符美英,陈绵才,肖彤斌等.根结线虫与寄主植物互作机理的研究进展[J].热带农业科学,2008,28(3)：73-74
[10]孙世伟,桑利伟.根结线虫防治研究进展[J].现代农业科技,2008,11：181-182
[11]张芸,胡铁军,师迎春.蔬菜根结线虫病药剂防治的实践体会[J]中国植保导刊,2004,24(10)：18-19
[12]孔凡彬,谢国红.茄子根结线虫发生规律及综合防治技术[J].安徽农业科学,2005,33(3)：446.
[13]蒋淑芝,张志斌,张真和.灌注热水消毒法防治设施蔬菜土传病虫害技术考察[J].中国蔬菜,2005,9：41
[14]房华,廖金铃,吴庆丽等.茶树菇菌渣对番茄根结线虫病防治的初步研究[J].莱阳农学院学报,2004,21(2)：182-184
[15]吴家琴,陈绵才,张怀芳.红麻生长期病虫害防治研究[J].中国麻作,1995,17(2)：25-28.
[16]马爱瑛,张靠稳,马岩根结线虫的生物防治[J].河北农业科学,200,12(2)：60-62
[17]崔林开,胡艳红,郭岩蔬菜根结线虫生物防治的研究进展[J].安徽农业科学,2006,34(22)：5907-5908
[18]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京：科学出版社,2002,1-20
[19]Botstein D.et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am.J.Hum.Genet,1980,32:314-316
[20]Williams J G, Kubelik A R, Livak K J. et al. DNA polymorphism amplified by arbitrary priMers are useful as genetic markers [J].Nucleic Acids Res,1990,18:6531-6535
[21]Welsh J, Meelelland M. Fingerprinting genomes using PCR with arbitray Primers [J]. Nueleie Aeids Res,1990,18:7213-7219
[22]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polyMerase chain reaction amplification[J].Genomics,1994,20:176-183
[23]Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers[J]. Nucleic Acids Research,1989,17:6463-6471
[24]Litt M, Luty J A. A hypervariable. Microsatellite revealed by in vitro amplification of dinucleotide repeat within the cardiac muscle actin gene[J].American Journal of Human Genetics,1989,44:397-401
[25]Zabeau M,Vos P.Selective restriction fragment amplification:a general Method for DNA fingerprinting,French,EP0534858[P].1992-09-24.
[26]Vos P,Hogers R,Bleeker M et al.A new technique for DNA fingerprinting[J].Nucleic Acids Research,1995,23(21):4407-4414.
[27]Konieczny A, Ausubel F M. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers[J]. Plant Journal,1993,4:403-410
[28]郝岗平,杨清,吴忠义等.植物的单核苷酸多态性及其在作物遗传育种中的应用[J].植物学通报,2004,21(5)：618-624
[29]Adams MD, Kelley JM, Gocayne JD et al. Complementary DNA Sequencing:Expressed Sequence Tags and Human GenoMe Project[J]. Science,1991,252:1651-1656,
[30]忻雅,崔海瑞,植物表达序列标签(EST)标记及其应用研究进展[J].生物学通报,2004,39(8)：4-6
[31]于凤池.EST技术及其应用综述[J].农业生物技术科学,2005,21(2)：54-58
[32]李小白,崔海瑞,张明龙等.EST分子标记开发及在比较基因组学中的应用[J].生物多样性,2006,14(6)：541-547
[33]Cordeiro GM, Casu R, McIntyre CL et al. Microsatellite markers from sugarcane(Saccharum spp.) ESTs cross transferable to Erianthus and Sorghum[J].Plant Science,2001,160:1115-1123
[34]Areshehenkova T, Ganal MW. Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources[J]. Theoretical and Applied Genetics,2002,104:229-235
[35]Scott KD, Eggler P, Seaton P et al. Analysis of SSRs derived from grape ESTs[J].Theor Appl Genet.2000,100:723-726,
[36]Eujayl I, Sorrells M, Baum M et al.Powell W Assessment of genotypic variation among cultivated durum wheat based on EST-SSR and genomic SSRs[J]. Euphytica,2001,119:39-43.
[37]Hackauf B.Wehling P. Identification of microsatellite polymorphisms in an expressed portion of the rye genoMe[J]. Plant Breed,2002,121:17-25
[38]Varshney RK, Sigmund R, Borner A et al. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice[J]. Plant Science,2005,168,195-202
[39]Song Q J,Marek L F,Shoemaker RC et al. A new integrated genetic linkage map of t he soybean[J]. Theor Appl Genet,2004,109:122-128
[40]Kantety R V, Rota M L, Matthews D E.et al. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat[J]. Plant Molecular Biology,2002, 48:501-510
[41]Cherdsak Liewlak saneeyanawin, Carol E Ritland, Yousry AEl-Kassaby et al. Single-copy, species-transferable micro satellite markers developed from loblolly pine ESTs[J].Theor Appl Genet,2004,109:361-369
[42]Malay C Saha,M A Rouf Mian,Imad Eujayl et al. Tall fescue EST-SSR markers with transferability across several grass species[J].Theor Appl Genet,2004,109:783-791
[43]Huang S.W., Zhang B.X., Milbourne D et al. Development of pepper SSR marker from sequence database [J], Euphytica,2000,117(2):163-167
[44]李晶晶,王述彬,刘金兵等.辣椒EST-SSR标记的开发[J].分子植物育种.2008,6(6)：1219-1222
[45]谢皓,陈学珍,杨柳等.EST-SSR标记的发展和在植物遗传研究中的应用[J].北京农学院学报,2005,20(4)：73-76
[46]邹礼平.分子标记在茄科蔬菜作物遗传育种研究中的应用[J].孝感学院学报(自然科学版),2000,20(4)：78-81
[47]黄三文，王晓武,张宝玺等.蔬菜作物分子标记研究进展与我国的发展策略[J].中国蔬菜,1999,1：50-53
[48]Wang Jauyueh. Study on the molecular markers of Capsicum wild domesticated species using RAPD analysis[J]. Journal of Agricultural Research of China,1966,45 (4):370-381
[49]Fan-Mingjen. Analysis of the genetic diversity of Capsicum ssp.(pepper) using RAPD analysis[J]. Journal of Agricultural Research of China,2001,50 (4):29-42
[50]JP Prince, VK Lackney,C Angeles et al. A survey of DNA polymorphism within the genus Capsicum and the fingerprinting of pepper cultivars [J]. Genome,1995,38 (2):224-231
[51]T Pradeepkumar,J L Karihaloo,Sunil Archak. Molecular characterization of Piper nigrum L.cultivars using RAPD markers[J]. Current Science,2001,81 (3):246-248
[52]I Paran, E Aftergoot,C Shifriss. Variation in Capsicum annuum revealed by RAPD and AFLP markers[J]. Euphytica,1998,99 (3):167-173
[53]V Lefebvre,B Goffinet,JC Chauvet et al. Evaluation of genetic distances between pepper inbred lines for cultivar protection purposes:comparison of AFLP, RAPD and Phenotypic data[J]. Theoretical and Applied Genetics,2001,102 (5):741-750
[54]J P Prince,E Pochard,SD Tanksley et al. Construction of a molecular linkage map of pepper and a comparison of synteny with tomato [J].Genome.1993,36(3):404-407
[55]A Ben chain, I Paran,RC Grube et al. QTL mapping of fruit related traits in pepper[J]. Theoretical and Applied Genetics,2001,102(6-7):1016-1028
[56]V Lefebvre,A Palloix,C Caranta et al. Construction of an intraspecific integrated map of pepper using molecular markers and doubled haploid progenies [J]. Genome,1995,38(1):112-121
[57]Yasuhiro Minamiyama,Masato Tsuro (?),Masashi Hirai. An SSR-based linkage map of Capsicum annuum [J].Mol Breeding,2006,18:157-169
[58]Han Paran, Jeroen Rouppe van der Voort et al. An integrated genetic linkage map of pepper (Capsicum spp.) [J], Molecular Breeding,2004,13:251-261
[59]Caranta C. Development of a CAPS marker for the Pvr4 locus a tool for pyramiding polyvirus resistance genes in pepper[J].Genome,1999,42(6):1111-1116
[60]Tai-T. High resolution genetic and physical mapping of the region containing the Bs2 resistance gene of pepper[J]. Theoretical and Applied Genetics,1999,99(7-8):1201-1206
[61]Pierre M. High resolution genetic mapping of the pepper resistance locus Bs3 governing recognition of the Xanthomonas Campestirs pv vesicatoria Avr Bs3 protein[J]. Theoretical and Applied Genetics,2000,101(1-2):255-263
[62]Choe Youngwon.Pepper (Capsicum annuum L) cultivar identification and seed purity test by RAPD and PGM isozyme RDA[J]. Journal of Horticulture Science,1998,40(1):15-23
[63]马艳青.分子标记技术及其在辣椒遗传育种上的应用[J].辣椒杂志(季刊),2004,3：1-8
[64]Moury B A.CAPS marker to assist selection of tomato spotted with virus resistance in pepper[J].Genome.2000,43(1):137-142
[65]Z Baoxi,H Sanwen,Y GuImei et al. Two RAPD markers linked to a major fetility restorer gene in pepper[J].Euphytica,2000,113(2):155-161
[66]MartinJA. Breeding of pungent peppers[J].South Carolina Agr. Expt.Sta.Annu.Rpt,1948,60:64-67
[67]Hare W W. Inheritance of resistance to root-knot nematodes in pepper [J].Phytopathology,1957,47: 455-459
[68]Martin J A, Crawford J H. Carolina Hot pepper[J]. South Carolina Agr. Expt. Sta.Cir.,1958:117
[69]黄三文,张宝玺,郭家珍等.辣(甜)椒根结线虫的危害、防治和抗病育种[J].园艺学报,2000,27(增刊)：515-521
[70]Hendy H, Pochard E, Dalmasso A. Transmission hereditaire de laresistance aux nematodes Meloidogyne Chitwood (Tylenchida) portee par 2 lignees de Capsicum annuum L:etude de descendances homozygotesissues d'androgenese[J].Agronomic,1985,5(2):93-100
[71]Bleve-Zacheo T, Bongiovanni M, Melillo M T et al. The pepper resistance genes Me1 and Me3 induce differential penetration rates and temporal sequences of root cellultra structural changes upon nematode infection[J]. Plant Science,1998,133:79-90
[72]C. Djian-Caporalino, A. Fazari,M. J. Arguel.et.al. Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome[J].Theor Appl Genet,2007,114:473-486
[73]Di Vito M,Zaccheo G,Catalano F.Effect of temperature on stability of resistance to root—knot nematode(Metoidogyne spp.)Ⅸth EUCARPIA meeting on Genetics and Breeding of Capsicum & Eggplant[J],Budapest,Hungary,1995,230-232
[74]顾晓慧,王立浩,毛胜利等.辣椒根结线虫防治与抗性育种研究进展[J].中国蔬菜,2006,5：33-36
[75]Vito M D,Saccardo F,Zaccheo G. Response of New Lines of Pepper to Meloidogyne incognita, M. javanica,M. arenaria and M. hapla[J]. Afro-Asian Journal of Nematology,1993,3(2):135-138
[76]Fery R L,Dukes P D Sr,Thies J A. Carolina Wonder and Charleston Belle:southern root-knot nematode-resistant bell peppers[J]. Hort-Science,1998,33(5):900-902
[77]C Djian-Caporalino-C,L Pijarowski,A Fazari et al.High-resolution genetic mapping of the pepper (Capsicum annuum L) resistance loci Me3 and Me4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.) [J]. Theor Appl Genet,2001,103:592-600
[78]顾晓慧.与辣椒抗根结线虫N基因紧密连锁的AFLP标记的开发[D].2006
[79]刘维志.病原植物线虫学[M].中国农业出版社,2000：372-425
[80]C Djian-Caporalino C, Pijarowski L, Januel A et al. Spectrum of resistance to root-knot nematodes and inheritance of heatstable resistance in pepper(Capsicum annuum L) [J].Theor Appl Genet, 1999,99:496-502
[81]陈海梅,李林志,卫宪云等.小麦EST-SSR标记的开发、染色体定位和遗传作图[J],科学通报,2005,50(20)：2208-2216
[82]SM Tam,C Mhiri,A Vogelaar et al. Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR[J].Theor Appl Genet,2005,110:819-831
[83]毛爱军,柴敏,于拴仓等.番茄抗根结线虫接种鉴定技术及其应用[J]西北农业学报.2005,14(4)：140-144
[84]Eujayl I, Sorrells ME, Baum M et al. Isolation of EST-derived microsatellite markers for genotyping the A and B genoMes of wheat[J]. Theor Appl Genet.2002,104:399-407
[85]Barrett B, Griffiths A, Schreiber M et al.A microsatellite map of white clover[J]. Theor Appl Genet, 2004,109:596-608
[86]Varshney R.K., Sigmund R., Korzun V et al Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice[J]. Plant Science,2005,168(1):195-202
[87]Han Z G, Guo W Z, Song X L et al.Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton[J]. Mol Genet Genomics,2004,272:308-327
[88]Gibum Yi, Je Min Lee, Sanghyeob Lee et al. Exploitation of pepper EST-SSRs and an SSR-based linkage map[J].Theor Appl Genet.2006,114:113-130
[89]胡勇胜,毛胜利,王立浩等.辣椒SSR反应体系优化[J],辣椒杂志,2007(2)：36-40
[90]Gupta P K,Rustgi S,Sharma S et al.Transferable EST-SSRs markers for the study of polymorphism and genetic diversity in bread wheat[J]. Mol.Gen.Genomics.2003,270 (4):315-323
[91]B. Jay a shreea, Ramu Punnab, P. Prasada.A Database of Simple Sequence Repeats from Cereal and Legume Expressed Sequence Tags Mined in silico:Survey and Evaluation In Silico[J] Bfology.2006,6:607-62
[92]Siva P.Kumpatla, Snehasis Mukhopadhyay. Mining and survey of simple sequence repeats in expressed sequence tags of dicotyledonous species [J]. Genome.2005,48:985-998
[93]Portis E., Nagy I., Sasvari Z et al. The design of Capsicum spp. SSR assays via analysis of in silico DNA sequence, and their potential utility for genetic mapping [J]. Plant Science.2007,172 (3): 640-648
[94]Mukund V. Katti, Prabhakar K. Ranjekar et al. Differential Distribution of Simple Sequence Repeats in Eukaryotic Genome Sequences[J]. Mol. Biol. Evol.2001,18 (7):1161-1167
[95]Jerzy Jurka, Charit Pethiyagoda. Simple repetitive DNA sequences from primates:Compilation and analysis [J]. Mol Evol,1995,40:120-126
[96]Linda Cardle, Luke Ramsay, Dan Milbourne et al. Computational and ExperiMental Characterization of Physically Clustered Simple Sequence Repeats in Plants[J]. Genetics.2000,156: 847-854
[97]Tsukasa Nunome, Satomi Negoro, Izumi Kono. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.)[J].Theor Appl Genet.2009, 119:1143-1153
[98]Jung Sook.Albert Abbott.Christopher Jesudurai.Frequency, type, distribution and annotation of simple sequence repeats in Rosaceae ESTs Funct Integr[J].Genomics.2005,5:136-143
[99]张俊娥,李芬,孙蒙祥烟草EST-SSR位点分析[J].武汉植物学研究.2007,25(5)：427-431
[100]Morgante M, Hanafey M, Powell W. Microsatellites are preferentially associated with nonrepeatitive DNA in plant genomes[J]. Nat.Genet.2002,30:194-200
[101]Mauricio La Rota, RaMesh V,Kantety Ju-Kyung Yuet al. Nonrandom distribution and frequencies of genomic and EST-derived microsatellite markers in rice, wheat, and barley[J]. BMC Genomics 2005,6:23
[102]B. Jayashreea,, Ramu Punnab, P. Prasada et al. A Database of simple sequence repeats from cereal and legume expressed sequence tags mined in silico survey and evaluation. In Silico[J]. Biology.2006,6:607-620
[103]N. Nicot,.V. Chiquet, B. Gandon.Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs)[J], Theor Appl Genet.2004,109:800-805
[104]T. Areshchenkova,M. W. Ganal. Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources[J]. Theor ApplGenet.2002,104：229-235
[105]Yinglei Lai, Fengzhu Sun.The Relationship between microsatellite slippage mutation rate and the number of repeat units[J]. Molecular Biology and Evolution.2003,20(12):2123-2131
[106]Xu X, PengM,Fang Z et al. The direction of microsatellite mutations is dependent upon allele length[J]. Nat Genet.2000,24:396-399
[107]骆蒙,贾继增.植物基因组表达序列标签(EST)计划研究进展[J].生物化学与生物物理进展,2001,28(4)：494-497
[108]C.Djian Caporalino, L.Pijarowski,A.Januel et al.Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome [J]. Theor Appl Genet (2007) 114:473-486
[109]Thies, J. A.,J. D. Mueller et al.Use of a resistant pepper as a rotational crop to manage southern root-knot nematode[J]. HortScience,1998,33:716-718.
[110]Thies J. A., Fery, R. L. Characterization of resistance conferred by the N gene to Meloidogyne arenaria races 1 and 2, M. hapla, and M. javanica in two sets of isogenic lines of Capsicum annuum L[J]. Journal of the American Society for Horticultural Science,2000,125,71-75
[111]Michelmore R.W, Paran, Kesseli R.V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:A rapid Method to detect markers in specific genomic regions by using segregating populations(random amplified polymorphic DNA/restriction fragMent length polymorphism)[J].Genetics Proc. Natl. Acad. Sci.1991,88(11):9828-9832,
[112]Fausto de Souza-Sobrinho, Wilson Roberto Maluf, Luiz A.A.et al.Inheritance of resistance to Meloidogyne incognita race 2 in the hot pepper cultivar Carolina Cayenne(Capsicum annuum L.)[J].Genetics and Molecular Research,2002,3:271-279
[113]茆振川,谢丙炎,杨宇红等.辣椒N基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析[J].园艺学报,2007,34(3)：629-636
[114]茆振川,谢丙炎,杨宇红等.辣椒Me3基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析[J].园艺学报,2007,34(6)：1453-1458
[115]Rugang Chen, Hanxia Li,Liying Zhang et al. CaMi, a root-knot nematode resistance gene from hot pepper (Capsium annuum L.) confers nematode resistance in tomato[J]. Plant Cell Rep,2007, 26:895-905
[116]Judy A. Thies. Jennifer J. Ariss. Comparison between the N and Me3 genes conferringresistance to the root-knot nematodes(Melodiogyne incognita) in genetically different pepper lines(Capsicum annuum)[J].Eur J Plant Pathol,2009,8:doi 10.1007/s10658-00909502-7
[117]L.H. Wang, X.H. Gu, M.Y. Hua et al.A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)[J].Scientia Horticulturae,2009(122),2:318-322
[118]A. Piedra Buena,J.A.Lopez-Perez, A. Bello et al.Screening of cuarentino pepper(capsicum annuum) for resistance to Meloidogyne incognita[J]. Nematropica,2006,36(1):13-22
[119]茆振川.辣椒与根结线虫不亲和互作相关基因的分离及功能分析[D].2007
[120]王添成.蔬菜抗病筛选技术[A].台湾省农业试验所：蔬菜育种技术研习会专刊,1998