一个水稻短生育期突变体的遗传分析与精细定位
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摘要
在优良迟熟恢复系明恢86的转基因后代中,发现了一个非T-DNA插入引起的短生育期突变体(暂命名short growth period,简称sgp(t))。该突变体对光周期反应不敏感,在不同生态区域与不同播种期,平均抽穗期40.9±2.1～62.4±5.2天,比野生型明恢86早35～50天。进一步分析sgp(t)突变体与野生型明恢86、93-11、闽恢3301和博白B等4个品种的杂种F2群体抽穗分布发现,分离群体后代中出现早抽穗、较早抽穗和迟抽穗3种类型,分离比率为1:2:1,符合一对基因的不完全显性遗传。以F2代(sgp(t)×93-11)中的早熟株和迟熟株为定位群体,应用分子标记将sgp(t)基因定位在第6染色体长臂的RM3628和RM439之间,随后利用已经公布的水稻基因组序列,在sgp(t)基因附近区域新开发了新的分子标记,将sgp(t)基因进一步定位在两个标记NSSR0699和NSSR0692之间,物理距离为75.8kb。定位结果显示sgp(t)不同于目前报道的所有早熟和迟熟基因,sgp(t)是一个控制水稻生育期的新基因。
A extremely short growth period mutant,which is tentatively named short growth period(sgp(t)),was found in the progenies of transgenic plants of Minghui 86,an lateness indica rice restorer line.Linkage analysis of transgenic marker gene and sgp(t)gene indicated that sgp(t)mutation was not caused by T-DNA insertion.Days to heading of homologous sgp(t)was 40.9±2.1～62.4±5.2 d,which was 35～50 d earlier than that of wildtype Minghui 86 in different times and ecological regions.The subsequent analysis of the F2 populations from the crosses sgp(t)and wildtype Minghui 86,93-11,Minhui 3301 and Bobai B,respectively,showed that the early heading of sgp(t)is governed by a semi-dominant mutant gene.The extremely earliness plants and lateness plants in F2 segregation populations derived from the cross sgp(t)/93-11 were used for molecular mapping of the sgp(t)gene using molecular markers.First,the sgp(t)locus was preliminarily mapped between marker RM3628 and marker RM439 in the region of the long arm of chromosome 6.Subsequently,new SSR and Indel markers were developed using published rice genome sequence and the gene was further mapped between markers NSSR0699 and NSSR0692,within 75.8 kb region The mapping result suggested that the sgp(t)gene differed from the other earliness and lateness genes in rice.So,the sgp(t)gene was a new gene controlling rice growth period.
A extremely short growth period mutant,which is tentatively named short growth period(sgp(t)),was found in the progenies of transgenic plants of Minghui 86,an lateness indica rice restorer line.Linkage analysis of transgenic marker gene and sgp(t)gene indicated that sgp(t)mutation was not caused by T-DNA insertion.Days to heading of homologous sgp(t)was 40.9±2.1～62.4±5.2 d,which was 35～50 d earlier than that of wildtype Minghui 86 in different times and ecological regions.The subsequent analysis of the F2 populations from the crosses sgp(t)and wildtype Minghui 86,93-11,Minhui 3301 and Bobai B,respectively,showed that the early heading of sgp(t)is governed by a semi-dominant mutant gene.The extremely earliness plants and lateness plants in F2 segregation populations derived from the cross sgp(t)/93-11 were used for molecular mapping of the sgp(t)gene using molecular markers.First,the sgp(t)locus was preliminarily mapped between marker RM3628 and marker RM439 in the region of the long arm of chromosome 6.Subsequently,new SSR and Indel markers were developed using published rice genome sequence and the gene was further mapped between markers NSSR0699 and NSSR0692,within 75.8 kb region The mapping result suggested that the sgp(t)gene differed from the other earliness and lateness genes in rice.So,the sgp(t)gene was a new gene controlling rice growth period.
引文
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