盾叶薯蓣中黄姜素A的研究和罗布麻叶中黄酮类物质的毛细管电泳分析
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摘要
1盾叶薯蓣(Dioscorea zingiberensis C.H.Wright)又名黄姜,为我国特有的野生药用植物资源。黄姜素A是从其中分离出的一种单一甾体类化合物。由于在盾叶薯蓣药材根茎中有很多结构相近的皂苷共存,所以黄姜素A对照品的制备十分困难,本论文对其提取分离方法进行了系统研究,研究比较了硅胶柱色谱、ODS硅胶柱色谱和高效制备液相色谱法三种不同的纯化方法,并起草了黄姜素A对照品的质量标准。
2罗布麻叶(Apocynum venetum L)是夹竹桃科植物罗布麻的干燥叶,是我国传统的平肝息风的中药。本论文采用毛细管区带电泳法(CZE)同时测定罗布麻叶中山奈酚、白麻苷、山奈酚-3-O-β-D-葡萄糖苷、异槲皮苷、槲皮素5种黄酮类化合物的含量,并比较了6个不同产区的各成分的含量差异。研究了缓冲溶液的组成、pH值、离子浓度对分离度和迁移时间的影响,得到了最佳分离实验条件。在离子浓度为50 mmol／L Na_2B_4O_7缓冲溶液(pH 9.3)中,分离电压为20 kV,检测波长340 nm时,山奈酚、白麻苷、山奈酚-3-O-β-D-葡萄糖苷、异槲皮苷、槲皮素5种黄酮类物质在15 min内得到了良好的分离测定,本方法简便、快速、灵敏、样品损耗少、试剂用量小、环境友好,为罗布麻叶的质量分析提供了新的分析方法。分析结果表明,白麻苷和异槲皮素苷是罗布麻叶中主要黄酮类成分,二者的含量最高达5.7‰和3‰。各产地药材以陕西、新疆产罗布麻黄酮类成分较多,且含量较高。
1 Dioscorea zingiberensis C.H.Wright, also known as Huangjiang,is a unique plant growing in China. Huangjiangsu A is a single steroid compounds which was isolated from it. As there are many saponins whose structures are quite similar to Huangjiangsu A exist in the rhizome of Dioscorea zingiberensis C.H.Wright, the isolation and purification of the reference substance of Huangjiangsu A are very difficult. In this paper, we did a detailed research on the methods of extraction and separation of Huangjiangsu A. Three methods of its purification: the silica gel column chromatography, ODS silica gel column chromatography and preparative high performance liquid chromatography, were compared,and the qulity standard of Huangjiangsu A was drafted.
2 Apocynum venetum belongs to Apocymaceae, Leaves of which are considered as a traditional, herbaL medicine.A capillary zone electrophoresis (CZE) method has been developed for the simultaneous determination of kaempferol, quercetin-3-O-β-D-glc(2→1)-β-D-glucose, kaempferol-3-O -β-D-glucose, isoquercitrin and quercetin in Apocynum Venetum leaves from different regions. The analytical conditions were optimized through investigation of the effects of buffer components, buffer pH and buffer concentration. These five flavonoids can be well-separated within 15 min at a working voltage of 20 kV and detection wavelength of 340 nm in a 50 mmol/L borate buffer (pH 9.3). The method is simple, rapid and sensitive with the small amount of sample and solvent required and ease of cleaning the column. Samples of Apocynum Venetum extracts were analyzed using the validated method,which is useful for its quality control. Through the determination by capillary zone electrophoresis (CZE), we found that quercetin-3-O-β-D-glucose and quercetin-3-O-β-D)-glc(2→1)-β-D-glucose are the main constituents of flavonoids in Apocynum venetum leaves, whose contents are up to 5.7‰and 3‰respectively. Flavones content is more and higher in Apocynum venetum leaves of Shan Xi province and Xin Jiang province than that of other habitats.
2罗布麻叶(Apocynum venetum L)是夹竹桃科植物罗布麻的干燥叶,是我国传统的平肝息风的中药。本论文采用毛细管区带电泳法(CZE)同时测定罗布麻叶中山奈酚、白麻苷、山奈酚-3-O-β-D-葡萄糖苷、异槲皮苷、槲皮素5种黄酮类化合物的含量,并比较了6个不同产区的各成分的含量差异。研究了缓冲溶液的组成、pH值、离子浓度对分离度和迁移时间的影响,得到了最佳分离实验条件。在离子浓度为50 mmol／L Na_2B_4O_7缓冲溶液(pH 9.3)中,分离电压为20 kV,检测波长340 nm时,山奈酚、白麻苷、山奈酚-3-O-β-D-葡萄糖苷、异槲皮苷、槲皮素5种黄酮类物质在15 min内得到了良好的分离测定,本方法简便、快速、灵敏、样品损耗少、试剂用量小、环境友好,为罗布麻叶的质量分析提供了新的分析方法。分析结果表明,白麻苷和异槲皮素苷是罗布麻叶中主要黄酮类成分,二者的含量最高达5.7‰和3‰。各产地药材以陕西、新疆产罗布麻黄酮类成分较多,且含量较高。
1 Dioscorea zingiberensis C.H.Wright, also known as Huangjiang,is a unique plant growing in China. Huangjiangsu A is a single steroid compounds which was isolated from it. As there are many saponins whose structures are quite similar to Huangjiangsu A exist in the rhizome of Dioscorea zingiberensis C.H.Wright, the isolation and purification of the reference substance of Huangjiangsu A are very difficult. In this paper, we did a detailed research on the methods of extraction and separation of Huangjiangsu A. Three methods of its purification: the silica gel column chromatography, ODS silica gel column chromatography and preparative high performance liquid chromatography, were compared,and the qulity standard of Huangjiangsu A was drafted.
2 Apocynum venetum belongs to Apocymaceae, Leaves of which are considered as a traditional, herbaL medicine.A capillary zone electrophoresis (CZE) method has been developed for the simultaneous determination of kaempferol, quercetin-3-O-β-D-glc(2→1)-β-D-glucose, kaempferol-3-O -β-D-glucose, isoquercitrin and quercetin in Apocynum Venetum leaves from different regions. The analytical conditions were optimized through investigation of the effects of buffer components, buffer pH and buffer concentration. These five flavonoids can be well-separated within 15 min at a working voltage of 20 kV and detection wavelength of 340 nm in a 50 mmol/L borate buffer (pH 9.3). The method is simple, rapid and sensitive with the small amount of sample and solvent required and ease of cleaning the column. Samples of Apocynum Venetum extracts were analyzed using the validated method,which is useful for its quality control. Through the determination by capillary zone electrophoresis (CZE), we found that quercetin-3-O-β-D-glucose and quercetin-3-O-β-D)-glc(2→1)-β-D-glucose are the main constituents of flavonoids in Apocynum venetum leaves, whose contents are up to 5.7‰and 3‰respectively. Flavones content is more and higher in Apocynum venetum leaves of Shan Xi province and Xin Jiang province than that of other habitats.
引文
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