电针对内囊电毁损痉挛模型大鼠中枢单胺类神经递质含量影响的研究
摘要
目的:本实验从大脑皮质、脊髓单胺类神经递质变化的角度,探讨针刺治疗内囊电毁损所致痉挛状态的作用机理,并为临床针刺治疗脑性损伤所致肌痉挛提供科学实验依据。
方法:以内囊电毁损痉挛模型SD大鼠为实验对象,将SD大鼠随机分为空白组、假手术组、模型组、电针督脉组、电针患侧组和电针健侧组。电针督脉组选用“大椎”和“命门”,电针患侧组选用患侧“曲池”和“阳陵泉”,健侧组选用健侧“曲池”和“阳陵泉”进行电针治疗。选用频率100Hz连续波,强度以大鼠肢体抖动为度,每天1次,每次30 min,连续治疗3天。运用BL-420e四道生理记录仪检测痉挛模型大鼠治疗前后肌张力的变化。运用荧光分光光度检测法分析观察不同部位电针对痉挛模型大鼠大脑皮质、脊髓单胺类神经递质去甲肾上腺素(NA)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响。
结果:1.内囊电毁损后,模型组、各针刺组与空白组、假手术组比较大鼠痉挛侧上肢伸直幅度明显降低,有统计学意义(p＜0.01);电针治疗后,各针刺组与模型组比较明显升高,有统计学意义(p＜0.01)。2.模型组与空白组、假手术组比较大脑皮质NA、DA、5—HT含量明显降低,有统计学意义(p＜0.05);各针刺组与模型组比较大脑皮质NA、DA、5—HT含量明显升高,有统计学意义(p＜0.05)。3.模型组与空白组、假手术组比较脊髓NA含量明显降低,有统计学意义(p＜0.05),各针刺组与模型组比较脊髓NA含量明显升高,有统计学意义(p＜0.05);模型组与空白组、假手术组比较脊髓DA含量有所增高,各电针组与模型组比较脊髓DA含量有所降低,但组间差异未显统计学意义(P＞0.05);模型组与空白组、假手术组比较脊髓5—HT含量有所降低,各电针组与模型组比较脊髓5—HT含量有所增高,但组间差异未显统计学意义(P＞0.05)。
结论:1.电针具有抗痉挛效应;
2.电针抗痉挛效应可能与调节中枢神经系统大脑皮质、脊髓单胺类神经递质水平有关;
3.在抗痉挛疗效和对中枢单胺类神经递质作用上,不同部位电针比较未见明显差异。
Objective To Study the effect of electro-acupuncture on monoamine neurotransmitter of cerebral cortex and caudex dorsalis in spasticity model rats by electrolytic damage of internal capsule, and to explore the mechanism of action of monoamine neurotransmitter and to provide experimental foundation to for acupuncture therapy of spasticity by brain injured on clinic.
Methods Establishd electrolytic damage of internal capsule model, then randomly individed SD rats into Blank group, Sham operation group, Electro-acupuncture DU Meridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group. Electro-acupuncture DU Meridian Group rats were treated with acupuncture on " Da zhui(DU14) and Ming men (DU4)". Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group rats were treated with acupuncture on " Qu chi(LI11) and Yang lingquan (GB34)".The treatment was once daily and 30 minutes everytime, lasting for 3 days by 100 Hz continuous wave to shake limbs lightly. Observing the influence of electric acupuncture on muscular tension of the spasmodic model rats by BL-420e 4-channels electrophysiolograph.Fluorospectrophotometry analysis technique were used to investigate the effect of electro-acupuncture on the contents of NA,DA and 5-HT in cerebral cortex and caudex dorsalis.
Results 1. After electrolytic damage of internal capsule ,Comparing with Blank group and Sham operation group, the extend amplitude of injured upper arm of rats in Model group and three electric acupuncture groups were decreased obviously (P < 0.01);After being treated,Comparing with Model group, the extend amplitude of injured upper arm of rats in three electric acupuncture groups were increased obviously (P < 0.01). 2. Comparing with Blank group and Sham operation group,the contents of NA,DA and 5-HT in the cerebral cortex of Model group were decreased obviously (P < 0.05);After being treated, comparing with Model group, the contents of NA,DA and 5-HT in the cerebral cortex of three electric acupuncture groups were increased obviously (P < 0.05).3. Comparing with Blank group and Sham operation group , the contents of NA in the caudex dorsalis of Model group were decreased obviously (P < 0.05) ,After being treated, compared with Model group , the contents of NA in the caudex dorsalis of three electric acupuncture groups were increased obviously (P < 0.05); Compareing with Blank group and Sham operation group, the contents of DA in the caudex dorsalis of Model group were increased,After being treated, compared with Model group , the contents of DA in the caudex dorsalis of three electric acupuncture groups were decreased; but not obvious varieties (P>0.05);Comparing with Blank group and Sham operation group , the contents of 5-HT in the caudex dorsalis of Model group were decreased,After being treated, comparing with Model group, the contents of DA in the caudex dorsalis of three electric acupuncture groups were increased, but not obvious varieties (P>0.05).
Conclusion 1. Spasticity could be resisted by electro-acupuncture. 2. The effect of resisting spasticity by electro-acupuncture may relate to accommodate the contents of NA, DA and 5-HT in cerebral cortex and caudex dorsalis. 3. Comparing Electro-acupuncture DUMeridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group each other, we founded the extend amplitude of injured upper arm of rats in three electric acupuncture groups did not have obvious difference., after being treated and the contents of NA,DA and 5-HT incerebral cortex and caudex dorsalis.did not have obvious varieties.
方法:以内囊电毁损痉挛模型SD大鼠为实验对象,将SD大鼠随机分为空白组、假手术组、模型组、电针督脉组、电针患侧组和电针健侧组。电针督脉组选用“大椎”和“命门”,电针患侧组选用患侧“曲池”和“阳陵泉”,健侧组选用健侧“曲池”和“阳陵泉”进行电针治疗。选用频率100Hz连续波,强度以大鼠肢体抖动为度,每天1次,每次30 min,连续治疗3天。运用BL-420e四道生理记录仪检测痉挛模型大鼠治疗前后肌张力的变化。运用荧光分光光度检测法分析观察不同部位电针对痉挛模型大鼠大脑皮质、脊髓单胺类神经递质去甲肾上腺素(NA)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响。
结果:1.内囊电毁损后,模型组、各针刺组与空白组、假手术组比较大鼠痉挛侧上肢伸直幅度明显降低,有统计学意义(p＜0.01);电针治疗后,各针刺组与模型组比较明显升高,有统计学意义(p＜0.01)。2.模型组与空白组、假手术组比较大脑皮质NA、DA、5—HT含量明显降低,有统计学意义(p＜0.05);各针刺组与模型组比较大脑皮质NA、DA、5—HT含量明显升高,有统计学意义(p＜0.05)。3.模型组与空白组、假手术组比较脊髓NA含量明显降低,有统计学意义(p＜0.05),各针刺组与模型组比较脊髓NA含量明显升高,有统计学意义(p＜0.05);模型组与空白组、假手术组比较脊髓DA含量有所增高,各电针组与模型组比较脊髓DA含量有所降低,但组间差异未显统计学意义(P＞0.05);模型组与空白组、假手术组比较脊髓5—HT含量有所降低,各电针组与模型组比较脊髓5—HT含量有所增高,但组间差异未显统计学意义(P＞0.05)。
结论:1.电针具有抗痉挛效应;
2.电针抗痉挛效应可能与调节中枢神经系统大脑皮质、脊髓单胺类神经递质水平有关;
3.在抗痉挛疗效和对中枢单胺类神经递质作用上,不同部位电针比较未见明显差异。
Objective To Study the effect of electro-acupuncture on monoamine neurotransmitter of cerebral cortex and caudex dorsalis in spasticity model rats by electrolytic damage of internal capsule, and to explore the mechanism of action of monoamine neurotransmitter and to provide experimental foundation to for acupuncture therapy of spasticity by brain injured on clinic.
Methods Establishd electrolytic damage of internal capsule model, then randomly individed SD rats into Blank group, Sham operation group, Electro-acupuncture DU Meridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group. Electro-acupuncture DU Meridian Group rats were treated with acupuncture on " Da zhui(DU14) and Ming men (DU4)". Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group rats were treated with acupuncture on " Qu chi(LI11) and Yang lingquan (GB34)".The treatment was once daily and 30 minutes everytime, lasting for 3 days by 100 Hz continuous wave to shake limbs lightly. Observing the influence of electric acupuncture on muscular tension of the spasmodic model rats by BL-420e 4-channels electrophysiolograph.Fluorospectrophotometry analysis technique were used to investigate the effect of electro-acupuncture on the contents of NA,DA and 5-HT in cerebral cortex and caudex dorsalis.
Results 1. After electrolytic damage of internal capsule ,Comparing with Blank group and Sham operation group, the extend amplitude of injured upper arm of rats in Model group and three electric acupuncture groups were decreased obviously (P < 0.01);After being treated,Comparing with Model group, the extend amplitude of injured upper arm of rats in three electric acupuncture groups were increased obviously (P < 0.01). 2. Comparing with Blank group and Sham operation group,the contents of NA,DA and 5-HT in the cerebral cortex of Model group were decreased obviously (P < 0.05);After being treated, comparing with Model group, the contents of NA,DA and 5-HT in the cerebral cortex of three electric acupuncture groups were increased obviously (P < 0.05).3. Comparing with Blank group and Sham operation group , the contents of NA in the caudex dorsalis of Model group were decreased obviously (P < 0.05) ,After being treated, compared with Model group , the contents of NA in the caudex dorsalis of three electric acupuncture groups were increased obviously (P < 0.05); Compareing with Blank group and Sham operation group, the contents of DA in the caudex dorsalis of Model group were increased,After being treated, compared with Model group , the contents of DA in the caudex dorsalis of three electric acupuncture groups were decreased; but not obvious varieties (P>0.05);Comparing with Blank group and Sham operation group , the contents of 5-HT in the caudex dorsalis of Model group were decreased,After being treated, comparing with Model group, the contents of DA in the caudex dorsalis of three electric acupuncture groups were increased, but not obvious varieties (P>0.05).
Conclusion 1. Spasticity could be resisted by electro-acupuncture. 2. The effect of resisting spasticity by electro-acupuncture may relate to accommodate the contents of NA, DA and 5-HT in cerebral cortex and caudex dorsalis. 3. Comparing Electro-acupuncture DUMeridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group each other, we founded the extend amplitude of injured upper arm of rats in three electric acupuncture groups did not have obvious difference., after being treated and the contents of NA,DA and 5-HT incerebral cortex and caudex dorsalis.did not have obvious varieties.
引文
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