二维相关红外光谱差异分析方法及其应用研究
|
摘要
复杂混合物体系的分析一直是相关领域的研究热点和重点,而“多级红外光谱宏观指纹分析法”已经逐步成为混合物整体分析的重要手段之一。红外光谱、二阶导数红外光谱和二维相关红外光谱构成的“红外光谱三级鉴别体系”,可以对组成复杂的相似样本进行有效区分。本论文以化学计量学理论为基础,通过模拟数据和实际样本的研究,初步建立了二维相关红外光谱差异分析的系统方法。
本论文首先总结了中药、食品等混合物二维相关红外光谱鉴别的系统化方法,包括计算理论、实验操作、谱图预处理、谱图归一化以及特征区域的选择。
本论文首次提出了二维相关红外光谱间的“距离”与“相关系数”、混合二维相关红外光谱的“对称距离”与“对称系数”等概念,解决了不同样本二维相关红外光谱的差异量化的问题。
本论文首次研究了二维相关红外光谱的差减与求导。对二维相关红外光谱进行差减,可以减弱绝对强度较大的峰,使弱峰更加显著;对二维相关红外光谱进行求导,可以分辨原谱的重叠峰,发掘更多被埋藏的信息;差减与求导均可让不同样本间的差异更加显著的表现出来,解决了高度相似样本的二维相关红外光谱分析和鉴别的问题。
本论文首次研究了二维相关红外光谱的统计量与主成分分析,初步解决了对大量样本的二维相关红外光谱进行分析和鉴别的问题。从一组样本的二维相关红外光谱的平均谱可以得知其共同特征,从标准差、偏度和峰度谱可以得知样本在哪些区域内的差异最显著。主成分分析的得分图可以对样本进行有效的分类,研究样本变化规律,从载荷图上则可以找到包含最有价值信息的区域。
本论文通过相关研究,建立了更为系统合理且客观量化的二维相关红外光谱间差异分析方法,为使用二维相关红外光谱对中药、食品等复杂混合物体系进行鉴别提供了系统的方法学指导。
Analysis of the complex mixture has always been the hotspot in the relevant research fields. Macro-fingerprints of multi-level infrared spectroscopy have been used for the global analysis of mixtures more and more today. Tri-level infrared spectroscopic identification, which employs the Fourier transform infrared spectroscopy (FT-IR), the second derivative infrared spectroscopy (SD-IR) and two-dimensional correlation infrared spectroscopy (2D-IR) to discriminate quite similar mixtures, has proved to be useful. The systematic methods to analysis the differences between 2D-IR spectra were developed by this research, based on chemometrics and both simulated and practical data.
The basic theory, experimental details, the preprocessing and normalization of the original spectra and the selection of regions for discriminating mixture samples such as traditional Chinese medicine (TCM) and food was summarized at first. The distance and correlation coefficient between 2D spectra, as well as the symmetry distance and symmetry coefficient of a hetero 2D spectrum, were introduced for the first time to evaluate the differences between 2D spectra quantitatively.
The differential and derivative 2DIR were also discussed for the first time by simulated and experimental data. Strong but useless peaks for the identification of samples would fade down on differential 2D spectra while the minor peaks varied much between different samples could be magnified. Overlapped peaks on original 2D spectra could be separated by the derivative processing and more information would come out. Both the differential and derivative 2DIR could make the differences between quite similar samples become much more significant.
The statistic and principal components analysis (PCA) of the 2DIR spectra were used for the first time to differentiate plenty of samples. The common features of the samples could be found by the mean 2D spectrum. The spectral regions where different samples could be discriminated effectively could be shown on the standard deviation, skewness and kurtosis of the 2D spectra. Samples could be clustered accurately by the scores plots and the most important spectral regions could be found by the loadings plots of the PCA analysis of the 2DIR spectra.
Methods for differentiating similar 2D spectra were well set up by this research. Guidance and techniques for the identification of mixtures such like the traditional Chinese medicine (TCM) and the food by 2DIR were provided.
本论文首先总结了中药、食品等混合物二维相关红外光谱鉴别的系统化方法,包括计算理论、实验操作、谱图预处理、谱图归一化以及特征区域的选择。
本论文首次提出了二维相关红外光谱间的“距离”与“相关系数”、混合二维相关红外光谱的“对称距离”与“对称系数”等概念,解决了不同样本二维相关红外光谱的差异量化的问题。
本论文首次研究了二维相关红外光谱的差减与求导。对二维相关红外光谱进行差减,可以减弱绝对强度较大的峰,使弱峰更加显著;对二维相关红外光谱进行求导,可以分辨原谱的重叠峰,发掘更多被埋藏的信息;差减与求导均可让不同样本间的差异更加显著的表现出来,解决了高度相似样本的二维相关红外光谱分析和鉴别的问题。
本论文首次研究了二维相关红外光谱的统计量与主成分分析,初步解决了对大量样本的二维相关红外光谱进行分析和鉴别的问题。从一组样本的二维相关红外光谱的平均谱可以得知其共同特征,从标准差、偏度和峰度谱可以得知样本在哪些区域内的差异最显著。主成分分析的得分图可以对样本进行有效的分类,研究样本变化规律,从载荷图上则可以找到包含最有价值信息的区域。
本论文通过相关研究,建立了更为系统合理且客观量化的二维相关红外光谱间差异分析方法,为使用二维相关红外光谱对中药、食品等复杂混合物体系进行鉴别提供了系统的方法学指导。
Analysis of the complex mixture has always been the hotspot in the relevant research fields. Macro-fingerprints of multi-level infrared spectroscopy have been used for the global analysis of mixtures more and more today. Tri-level infrared spectroscopic identification, which employs the Fourier transform infrared spectroscopy (FT-IR), the second derivative infrared spectroscopy (SD-IR) and two-dimensional correlation infrared spectroscopy (2D-IR) to discriminate quite similar mixtures, has proved to be useful. The systematic methods to analysis the differences between 2D-IR spectra were developed by this research, based on chemometrics and both simulated and practical data.
The basic theory, experimental details, the preprocessing and normalization of the original spectra and the selection of regions for discriminating mixture samples such as traditional Chinese medicine (TCM) and food was summarized at first. The distance and correlation coefficient between 2D spectra, as well as the symmetry distance and symmetry coefficient of a hetero 2D spectrum, were introduced for the first time to evaluate the differences between 2D spectra quantitatively.
The differential and derivative 2DIR were also discussed for the first time by simulated and experimental data. Strong but useless peaks for the identification of samples would fade down on differential 2D spectra while the minor peaks varied much between different samples could be magnified. Overlapped peaks on original 2D spectra could be separated by the derivative processing and more information would come out. Both the differential and derivative 2DIR could make the differences between quite similar samples become much more significant.
The statistic and principal components analysis (PCA) of the 2DIR spectra were used for the first time to differentiate plenty of samples. The common features of the samples could be found by the mean 2D spectrum. The spectral regions where different samples could be discriminated effectively could be shown on the standard deviation, skewness and kurtosis of the 2D spectra. Samples could be clustered accurately by the scores plots and the most important spectral regions could be found by the loadings plots of the PCA analysis of the 2DIR spectra.
Methods for differentiating similar 2D spectra were well set up by this research. Guidance and techniques for the identification of mixtures such like the traditional Chinese medicine (TCM) and the food by 2DIR were provided.
引文
[1]孙素琴,张宣,秦竹,等. FTIR直接鉴别植物生药材.光谱学与光谱分析, 1999, 19: 542-545.
[2]孙素琴,郁鉴源,胡鑫尧.分子光谱法无损鉴别生药材的最新进展.光谱学与光谱分析, 1999, 19: 841-843.
[3]孙素琴,周群,郁鉴源,等.分子振动光谱法与中药研究的最新进展.光谱学与光谱分析, 2000, 20: 199-202.
[4]孙素琴,周群,秦竹.中药注射剂红外光谱非分离提取多级宏观指纹鉴定:中国, ZL03104765.3. (中国专利公开号)
[5]孙素琴,周群,秦竹,等.中药配方颗粒红外光谱非分离提取多级宏观指纹鉴定方法:中国, ZL03122935.2. (中国专利公开号)
[6]孙素琴,秦竹,周群.中药药材红外光谱非分离提取多级宏观指纹鉴定:中国, ZL03123389.9. (中国专利公开号)
[7] Cho M. Coherent two-dimensional optical spectroscopy. Chem. Rev. 2008, 108: 1331-1418.
[8] Noda I. Two-dimensional infrared spectroscopy of synthetic and biopolymers. Bull. Am. Phys. Soc. 1986, 31: 520.
[9] Noda I. Generalized two-dimensional infrared spectroscopy. J. Am. Chem. Soc. 1989, 111: 8116-8118.
[10] Noda I. Two-dimensional infrared (2D IR) spectroscopy: theory and applications. Appl. Spectros. 1990, 44: 550-561.
[11] Noda I. Generalized two-dimensional correlation method applicable to infrared, raman, and other types of spectroscopy. Appl. Spectrosc. 1993, 47: 1329-1336.
[12] Noda I, Ozaki Y. Two-dimensional correlation spectroscopy - applications in vibrational and optical spectroscopy. Chichester: Wiley, 2004.
[13]孙素琴,周群,秦竹.中药二维相关红外光谱鉴定图集.北京:化学工业出版社, 2003.
[14] Noda I. Advances in two-dimensional correlation spectroscopy. Vib. Spectrosc. 2004, 36: 143-165.
[15] Noda I. Progress in two-dimensional (2D) correlation spectroscopy. J. Mol. Struct. 2006, 799: 2-15.
[16] Noda I. Recent advancement in the field of two-dimensional correlation spectroscopy. J. Mol. Struct. 2008, 883-884: 2-26.
[17] Czarnecki M A. Some comments on the application of two-dimensional correlation spectroscopy and normalization of the dynamic spectra. Appl. Spectrosc. 2003, 57: 107-109.
[18] Jung Y M. Principal component analysis based two-dimensional correlation spectroscopy for noise filtering effect. Vib. Spectrosc. 2004, 36: 267-270.
[19] (?)a(?)ic S, Muszynski A, Ozaki Y. A new possibility of the generalized two-dimensional correlation spectroscopy. 1. Sample-sample correlation spectroscopy. J. Phys. Chem. A. 2000, 104: 6380-6387.
[20] (?)a(?)ic S, Muszynski A, Ozaki Y. A new possibility of the generalized two-dimensional correlation spectroscopy. 2. Sample-sample and wavenumber-wavenumber correlations of temperature-dependent near-infrared spectra of oleic acid in the pure liquid state. J. Phys. Chem. A. 2000, 104: 6388-6394.
[21] Ferreira A P, Menezes J C. Monitoring a complex medium fermentation with sample-sample two-dimensional FT-NIR correlation spectroscopy. Biotechnol. Prog. 2006, 22: 866-872.
[22] Hu Y, Zhang Y, Li B, et al. Application of sample–sample two-dimensional correlation spectroscopy to determine the glass transition temperature of poly(ethylene terephthalate) thin films. Appl. Spectrosc. 2007, 61: 60-67.
[23] Wang L X, Wu Y Q, Meersman F. Clarification of the thermally-induced pretransition of ribonuclease A in solution by principal component analysis and two-dimensional correlation infrared spectroscopy. Vib. Spectrosc. 2006, 42: 201-205.
[24] Wang L X, Wu Y Q, Meersman F. Two-dimensional correlation infrared spectroscopic study on the conformational changes occurring in the thermally induced pretransition of ribonuclease A. J. Mol. Struct. 2006, 799: 85-90.
[25] Wu Y Q, Jiang J H, Ozaki Y. A new possibility of generalized two-dimensional correlation spectroscopy: hybrid two-dimensional correlation spectroscopy. J. Phys. Chem. A. 2002, 106: 2422-2429.
[26] Wu Y Q, Meersman F, Ozaki Y. A novel application of hybrid two-dimensional correlation infrared spectroscopy: exploration of the reversibility of the pressure- and temperature-induced phase separation of poly(N-isopropylacrylamide) and poly (N-isopropylmethacrylamide) in aqueous solution. Macromolecules 2006, 39: 1182-1188.
[27] Noda I. Two-dimensional correlation approach to the dynamic rheooptical characterization of polymers. Chemtract-Macromol. Chem. 1990, 1: 89-105.
[28] Muik B, Lendl B, Molina-Diaz A, et al. Two-dimensional correlation spectroscopy and multivariate curve resolution for the study of lipid oxidation in edible oils monitored by FTIR and FT-Raman spectroscopy. Anal. Chim. Acta. 2007, 593: 54-67.
[29] Hu B W, Zhou P, Noda I, et al. Generalized two-dimensional correlation analysis of NMR and raman spectra for structural evolution characterizations of silk fibroin. J. Phys. Chem. B. 2006, 110: 18046-18051.
[30] Jung Y M, Czarnik-Matusewicz B, Ozaki Y. Two-dimensional infrared, two-dimensional Raman, and two-dimensional infrared and Raman heterospectral correlation studies of secondary structure of beta-lactoglobulin in buffer solutions. J. Phys. Chem. B. 2000, 104: 7812-7817.
[31] Thomas M, Richardson H. FTIR investigation of the interaction of tumor cells treated with caffeic acid and chlorogenic acid. Vib. Spectrosc. 2000, 24: 225-231.
[32] Morita S, Shinzawa H, Noda I, et al. Effect of band position shift on moving-window two-dimensional correlation spectroscopy. J. Mol. Struct. 2006, 799: 16-22.
[33] Morita S, Shinzawa H, Noda I, et al. Perturbation-correlation moving-window two-dimensional correlation spectroscopy. Appl. Spectrosc. 2006, 60: 398-406.
[34] Watanabe A, Morita S, Ozaki Y. Temperature-dependent structural changes in hydrogen bonds in microcrystalline cellulose studied by infrared and near-infrared spectroscopy with perturbation-correlation moving-window two-dimensional correlation analysis. Appl. Spectrosc. 2006, 60: 611-618.
[35] Zhou Q, Sun S Q, Zhan D Q, et al. Point-point and point-line moving-window correlation spectroscopy and its applications. J. Mol. Struct. 2008, 883-884: 109-115.
[36] Barton F E, Himmelsbach D S, Duckworth J H, et al. Two-dimensional vibration spectroscopy: correlation of mid- and near-infrared regions. Appl. Spectrosc. 1992, 46: 420-429.
[37] Barton F E, Himmelsbach D S. Two-dimensional vibration spectroscopy II: correlation of the absorptions of lignins in the mid- and near-infrared. Appl. Spectrosc. 1993, 47: 1920-1925.
[38] Pielesz A, Wesleucha-Birczynska A, Freeman H S, et al. Characterizing of model direct dyes interactions with cotton cellulose via 1D and 2D raman spectroscopy. Cellulose 2005, 12: 497-506.
[39] Moore A A, Jacobson M L, Belabas N, et al. 2D correlation analysis of the continuum in single molecule surface enhanced raman spectroscopy. J. Am. Chem. Soc. 2005, 127: 7292-7293.
[40] Elmore D L, Dluhy R.βν-Correlation Analysis: A modified two-dimensional infrared correlation method for determining relative rates of intensity change. J. Phys. Chem. B. 2001, 105: 11377-11386.
[41] Morita S, Ozaki Y, Noda I. Global phase angle description of generalized two-dimensional correlation spectroscopy: 1. theory and its simulation for practical use. Appl. Spectrosc. 2001, 55: 1618-1621.
[42] Noda I. Kernel analysis for two-dimensional (2D) correlation spectroscopy. J. Mol. Struct. 2006, 799: 34-40.
[43] Czarnecki M A, Wu PY, Siesler H W. 2D FT-NIR and FT-IR correlation analysis of temperature-induced changes of nylon12. Chem. Phys. Lett. 1998, 83: 326-332.
[44] Wu P Y, Siesler H W. Two-dimensional correlation analysis of variable temperature Fourier-transform mid and near-infrared spectra of plyamide11. J. Mol. Struc. 2000, 521: 37-47.
[45] Wu P Y, Yang Y L, Siesler H W. Two-dimentional near-infrared correlation temperature studies of an amorphous Polyamide. Polymer. 2001, 42: 10181-10186.
[46] Amari T, Ozaki Y. Generalized two-dimensional attenuated total reflection/infrared and near-infrared correlation spectroscopy studies of real-time monitoring of the initial oligomerization of bis(hydroxyethyl terephthalate). Macromolecules 2002, 35: 8020-8028.
[47] Shen Y, Chen F E, Wu P Y, et al. A two-dimensional Raman spectroscopic study on the structural changes of a polythiophene film during the cooling process. J. Chem. Phys. 2003, 119: 11415-11419.
[48] Kim H, Kim S B, Kim, J K, et al. Phase behavior of a weakly interacting block copolymer by temperature-dependent FTIR spectroscopy. Macromolecules 2006, 39: 408-412.
[49] Xu Y, Wu P Y. A study of water dehydration in nylon 6 as a function of temperature using two-dimensional (2D) correlation near-infrared (NIR) analysis. J. Mol. Struct. 2007, 833: 145-149.
[50] Wan L S, Huang X J, Xu Z K. Diffusion and structure of water in polymers containing N-vinyl-2-pyrrolidone. J. Phys. Chem. B. 2007, 111: 922-928.
[51] Thomas M, Richardson H. Two-dimensional FT-IR correlation analysis of the phase transitions in a liquid crystal, 4X-n-octyl-4-cyanobiphenyl (8CB). Vib. Spectrosc. 2000, 24: 137-146.
[52] Popescu M C, Filip D, Vasile C, et al. Characterization by Fourier transform infrared spectroscopy (FT-IR) and 2D IR correlation spectroscopy of PAMAM dendrimer. J. Phys. Chem. B. 2006, 110: 14198-14211.
[53] Jung Y M, Shin H S, Czarnik-Matusewicz B, et al. Characterization of transition temperatures of a Langmuir-Blodgett film of poly (tert-butyl methacrylate) by two-dimensional correlation spectroscopy and principal component analysis. Appl. Spectrosc. 2002, 56: 1568-1574.
[54] Azizan S, Shibata K, Matsuda T, et al. Surface phase transition of C12E1 at the air/water interface: a study by dynamic surface tension, external RA FT-IR, and 2D IR correlation methods. J. Phys. Chem. B. 2006, 110: 17034-17042.
[55] Sallach R, Wei M, Biswas N, et al. Micelle density regulated by a reversible switch of protein secondary structure. J. Am. Chem. Soc. 2006, 128: 12014-12019.
[56] Ashton L, Czarniz-Matusewicz B, Blanch E W. Application of two-dimensional correlation analysis to Raman optical activity. J. Mol. Struct. 2006, 799: 61-71.
[57] Yan Y B, Zhang J, He H W, et al. Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy. Biophys. J. 2006, 90: 2525-2533.
[58] Wang G, Gao Y, Geng M L. Generalized two-dimensional heterocorrelation analysis of spectrally resolved and temporally resolved fluorescence of the 8-anilino-1-naphthalenesulfonate-apomyoglobin complex with pH perturbation. J. Phys. Chem. B. 2006, 110: 8506-8512.
[59] Zhang J, He H W, Wang Q, et al. Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy. Protein Pept. Lett. 2006, 13: 33-40.
[60] Otsuka M, Fukui Y, Otsuka K, et al. Comparative evaluation of bioactivity change of crystalline trypsin during compression by chemoinformatics and 2D Fourier-transform infrared spectroscopy. Analyst 2006, 131: 1116-1121.
[61] Lehmann N, Alexiev U, Fahmy K. Linkage between the intramembrane H-bond network around aspartic acid 83 and the cytosolic environment of helix 8 in photoactivated rhodopsin. J. Mol. Biol. 2007, 366: 1129-1141.
[62] Hu B W, Zhou P, Noda I, et al. An NMR approach applicable to biomolecular structure characterization. Anal. Chem. 2005, 77: 7534-7538.
[63] Watanabe A, Morita S, Ozaki Y. Study on temperature-dependent changes in hydrogen bonds in cellulose Iβby infrared spectroscopy with perturbation-correlation moving-window two-dimensional correlation spectroscopy. Biomacromolecules 2006, 7: 3164-3170.
[64] Verma D, Katti K, Katti D. Nature of water in nacre: A 2D Fourier transform infrared spectroscopic study. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2007, 67: 784-788.
[65] Yakimets I, Paes S S, Wellner N, et al. Effect of water content on the structural reorganization and elastic properties of biopolymer films: a comparative study. Biomacromolecules 2007, 8: 1710-1722.
[66] DeGelder J, DeGussem K, Vandenabeele P, et al. Methods for extracting biochemical information from bacterial Raman spectra: An explorative study on Cupriavidus metallidurans. Anal. Chim. Acta 2007, 585: 234-240.
[67] Yu L, Sun SQ, Fan KF, et al. Research on processing medicinal herbs with multi-steps infrared macro-fingerprint method. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2005, 62: 22-29.
[68] Xu C H, Sun S Q, Guo C Q, et al. Multi-steps infrared macro-fingerprint analysis for thermal processing of Fructus viticis. Vib. Spectrosc. 2006, 41: 118-125.
[69] Wu Y W, Sun S Q, Zhou Q, et al. Fourier transform mid-infrared (MIR) and near-infrared (NIR) spectroscopy for rapid quality assessment of Chinese medicine preparation Honghua Oil. J. Pharm. Biomed. Anal. 2008, 46: 498-504.
[70] Czarnik-Matusewicz B, Murayama K, Tsenkova R, et al. Analysis of near-infrared spectra of complicated biological fluids by two-dimensional correlation spectroscopy: Protein and fat concentration-dependent spectral changes of milk. Appl. Spectrosc. 1999, 53: 1582-1594.
[71] Liu Y L, Barton F E, Lyon B G, et al. Two-dimensional correlation analysis of visible/near-infrared spectral intensity variations of chicken breasts with various chilled and frozen storages. J. Agric. Food Chem. 2004, 52: 505-510.
[72] Sun S Q, Li C W, Wei J P, et al. Discrimination of chinese sauce liquor using FT-IR and two-dimensional correlation IR spectroscopy. J. Mol. Struct. 2006, 799: 72-76.
[73] Zhou Q, Sun S Q, Yu L, et al. Sequential changes of main components in different kinds of milk powders using two-dimensional infrared correlation analysis. J. Mol. Struct. 2006, 799: 77-84.
[74] Kizil R, Irudayaraj J. Rapid evaluation and discrimination of gamma-irradiated carbohydrates using FT-Raman spectroscopy and canonical discriminant analysis. J. Sci. Food Agric. 2003, 87: 1244-1251.
[75] Wynne L, Clark S, Adams M J, et al. Compositional dynamics of a commercial wine fermentation using two-dimensional FTIR correlation analysis. Vib. Spectrosc. 2007, 44: 394-400.
[76] Nagai N, Yamaguchi Y, Saito R, et al. IR and EPR study of the Na ion-limplanted SiO2/Si system. Appl. Spectrosc. 2001, 55: 1207-1213.
[77] Choi H C, Jung Y M, Noda I, et al. A study of the mechanism of the electrochemical reaction of lithium with CoO by two-dimensional soft X-ray absorption spectroscopy (2D XAS), 2D Raman, and 2D heterospectral XAS-Raman correlation analysis. J. Phys. Chem. B 2003, 107: 5806-5811.
[78] Czarnik-Matusewicz B, Pilorz S, Hawranek J P. Temperature-dependent water structural transitions examined by near-IR and mid-IR spectra analyzed by multivariate curve resolution and two-dimensional correlation spectroscopy. Anal. Chim. Acta 2005, 544: 15-25.
[79] Sasaki T, Itoh J, Horiguchi Y, et al. Quantitative determination of corrosion products and adsorbed water on copper in humid air containing SO2 by IR-RAS measurements. Corros. Sci. 2006, 48: 4339-4351.
[80] Noda I, Liu Y L, Ozaki Y, et al. Two-dimensional fourier-transform near-infrared correlation spectroscopy studies of temperature-dependent spectral variations of oleyl alcohol. J. Phys.
[81] Noda I, Marcott C. Two-dimensional raman (2D Raman) correlation spectroscopy study of non-oxidative photodegradation ofβ-carotenet. J. Phys. Chem. A 2002, 106: 3371-3376.
[82] Zhong Z, Wang G, Geng M L. Probing strong adsorption of individual solute molecules at solid/liquid interfaces with model-based statistical two-dimensional correlation analysis. J. Mol. Struct. 2006, 799: 204-210.
[83] Nakashima K, Fukuma H, Ozaki Y, et al. Two-dimensional correlation fluorescence spectroscopy V: Polarization perturbation as a new technique to induce intensity changes in fluorescence spectra. J. Mol. Struct. 2006, 799: 52-55.
[84] Chen Y, Zhang H, Huang C, et al. Synthesis and two-dimensional correlation infrared spectroscopy study of an organic–inorganic hybrid compound constructed by [Mo5P2O23] and Cu-imidazole components. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2006, 63: 536-540.
[85] Popescu M C, Zanoaga M, Mamunya Y, et al. Two dimensional infrared correlation spectroscopy studies of wood-plastic composites with a copolyamide as matrix. J. Optoelectron. Adv. Mater. 2007, 9: 923-930.
[86] Hua R, Sun S Q, Zhou Q, et al. Discrimination of Fritillary according to geographical origin with Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. J. Pharm. Biomed. Anal. 2003, 33: 199-209.
[87] Liu Y, Wang J Q, Liu S H, et al. Two-dimensional correlation infrared spectroscopy applied to analyzing and identifying the Radix paeoniae Alba medicinal materials. J. Mol. Struct. 2008, 883-884: 137-141.
[88] Zhou Q, Sun S Q, Zuo L. Study on traditional Chinese medicine 'Qing Kai Ling' injections from different manufactures by 2D IR correlation spectroscopy. Vib. Spectrosc. 2004, 36: 207-212.
[89] Wu Y W, Sun S Q, Zhou Q, et al. Volatility-dependent 2D IR correlation analysis of traditional Chinese medicine 'Red Flower Oil' preparation from different manufacturers. J. Mol. Struct. 2008, 882: 107-115.
[90] Li Y M, Sun S Q, Zhou Q, et al. Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy. Vib. Spectrosc. 2004, 36: 227-232.
[91] Lu G H, Zhou Q, Sun S Q, et al. Differentiation of Asian ginseng, American ginseng and Notoginseng by Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy. J. Mol. Struct. 2008, 883-884: 91-98.
[92] Liu D, Li Y G, Xu H, et al. Differentiation of the root of cultivated ginseng, mountain cultivated ginseng and mountain wild ginseng using FT-IR and two-dimensional correlation IR spectroscopy. J. Mol. Struct. 2008, 883-884: 228-235.
[93]詹达琦,张晓明,孙素琴.基于小波变换的二维红外相关光谱鉴别人参的生长年限.光谱学与光谱分析. 2007, 27: 1497-1501.
[94] Liu H X, Sun S Q, Lv G H, et al. Discrimination of extracted lipophilic constituents of Angelica with multi-steps infrared macro-fingerprint method. Vib. Spectrosc. 2006, 40: 202-208.
[95] Liu H X, Sun S Q, Lv G H, et al. Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2006, 64: 321-326.
[96] Liu H X, Zhou Q, Sun S Q, et al. Discrimination of different Chrysanthemums with Fourier transform infrared spectroscopy. J. Mol. Struct. 2008, 883-884: 38-47.
[97]周晶,孙建云,徐胜艳,等.八角茴香与其伪品莽草的红外光谱三级鉴定研究.光谱学与光谱分析. 2008, 28: 2864-2867.
[98]刘军,周群,孙素琴.柴胡及其伪品大叶柴胡红外光谱的分析与鉴定.光谱学与光谱分析. 2008, 28: 107-108.
[99]阿依古丽,周群,董晓鸥,等.甘草真伪品的FTIR光谱法鉴别研究.光谱学与光谱分析. 2006, 26: 1238-1241.
[100]金哲雄,徐胜艳,孙素琴,等.刺五加不同部位的红外光谱分析与鉴定.光谱学与光谱分析. 2008, 28: 2859-2863.
[101]徐荣,孙素琴,刘友刚,等.红外光谱法对肉苁蓉径向不同部位的分析与评价.分析化学. 2009, 37: 221-226.
[2]孙素琴,郁鉴源,胡鑫尧.分子光谱法无损鉴别生药材的最新进展.光谱学与光谱分析, 1999, 19: 841-843.
[3]孙素琴,周群,郁鉴源,等.分子振动光谱法与中药研究的最新进展.光谱学与光谱分析, 2000, 20: 199-202.
[4]孙素琴,周群,秦竹.中药注射剂红外光谱非分离提取多级宏观指纹鉴定:中国, ZL03104765.3. (中国专利公开号)
[5]孙素琴,周群,秦竹,等.中药配方颗粒红外光谱非分离提取多级宏观指纹鉴定方法:中国, ZL03122935.2. (中国专利公开号)
[6]孙素琴,秦竹,周群.中药药材红外光谱非分离提取多级宏观指纹鉴定:中国, ZL03123389.9. (中国专利公开号)
[7] Cho M. Coherent two-dimensional optical spectroscopy. Chem. Rev. 2008, 108: 1331-1418.
[8] Noda I. Two-dimensional infrared spectroscopy of synthetic and biopolymers. Bull. Am. Phys. Soc. 1986, 31: 520.
[9] Noda I. Generalized two-dimensional infrared spectroscopy. J. Am. Chem. Soc. 1989, 111: 8116-8118.
[10] Noda I. Two-dimensional infrared (2D IR) spectroscopy: theory and applications. Appl. Spectros. 1990, 44: 550-561.
[11] Noda I. Generalized two-dimensional correlation method applicable to infrared, raman, and other types of spectroscopy. Appl. Spectrosc. 1993, 47: 1329-1336.
[12] Noda I, Ozaki Y. Two-dimensional correlation spectroscopy - applications in vibrational and optical spectroscopy. Chichester: Wiley, 2004.
[13]孙素琴,周群,秦竹.中药二维相关红外光谱鉴定图集.北京:化学工业出版社, 2003.
[14] Noda I. Advances in two-dimensional correlation spectroscopy. Vib. Spectrosc. 2004, 36: 143-165.
[15] Noda I. Progress in two-dimensional (2D) correlation spectroscopy. J. Mol. Struct. 2006, 799: 2-15.
[16] Noda I. Recent advancement in the field of two-dimensional correlation spectroscopy. J. Mol. Struct. 2008, 883-884: 2-26.
[17] Czarnecki M A. Some comments on the application of two-dimensional correlation spectroscopy and normalization of the dynamic spectra. Appl. Spectrosc. 2003, 57: 107-109.
[18] Jung Y M. Principal component analysis based two-dimensional correlation spectroscopy for noise filtering effect. Vib. Spectrosc. 2004, 36: 267-270.
[19] (?)a(?)ic S, Muszynski A, Ozaki Y. A new possibility of the generalized two-dimensional correlation spectroscopy. 1. Sample-sample correlation spectroscopy. J. Phys. Chem. A. 2000, 104: 6380-6387.
[20] (?)a(?)ic S, Muszynski A, Ozaki Y. A new possibility of the generalized two-dimensional correlation spectroscopy. 2. Sample-sample and wavenumber-wavenumber correlations of temperature-dependent near-infrared spectra of oleic acid in the pure liquid state. J. Phys. Chem. A. 2000, 104: 6388-6394.
[21] Ferreira A P, Menezes J C. Monitoring a complex medium fermentation with sample-sample two-dimensional FT-NIR correlation spectroscopy. Biotechnol. Prog. 2006, 22: 866-872.
[22] Hu Y, Zhang Y, Li B, et al. Application of sample–sample two-dimensional correlation spectroscopy to determine the glass transition temperature of poly(ethylene terephthalate) thin films. Appl. Spectrosc. 2007, 61: 60-67.
[23] Wang L X, Wu Y Q, Meersman F. Clarification of the thermally-induced pretransition of ribonuclease A in solution by principal component analysis and two-dimensional correlation infrared spectroscopy. Vib. Spectrosc. 2006, 42: 201-205.
[24] Wang L X, Wu Y Q, Meersman F. Two-dimensional correlation infrared spectroscopic study on the conformational changes occurring in the thermally induced pretransition of ribonuclease A. J. Mol. Struct. 2006, 799: 85-90.
[25] Wu Y Q, Jiang J H, Ozaki Y. A new possibility of generalized two-dimensional correlation spectroscopy: hybrid two-dimensional correlation spectroscopy. J. Phys. Chem. A. 2002, 106: 2422-2429.
[26] Wu Y Q, Meersman F, Ozaki Y. A novel application of hybrid two-dimensional correlation infrared spectroscopy: exploration of the reversibility of the pressure- and temperature-induced phase separation of poly(N-isopropylacrylamide) and poly (N-isopropylmethacrylamide) in aqueous solution. Macromolecules 2006, 39: 1182-1188.
[27] Noda I. Two-dimensional correlation approach to the dynamic rheooptical characterization of polymers. Chemtract-Macromol. Chem. 1990, 1: 89-105.
[28] Muik B, Lendl B, Molina-Diaz A, et al. Two-dimensional correlation spectroscopy and multivariate curve resolution for the study of lipid oxidation in edible oils monitored by FTIR and FT-Raman spectroscopy. Anal. Chim. Acta. 2007, 593: 54-67.
[29] Hu B W, Zhou P, Noda I, et al. Generalized two-dimensional correlation analysis of NMR and raman spectra for structural evolution characterizations of silk fibroin. J. Phys. Chem. B. 2006, 110: 18046-18051.
[30] Jung Y M, Czarnik-Matusewicz B, Ozaki Y. Two-dimensional infrared, two-dimensional Raman, and two-dimensional infrared and Raman heterospectral correlation studies of secondary structure of beta-lactoglobulin in buffer solutions. J. Phys. Chem. B. 2000, 104: 7812-7817.
[31] Thomas M, Richardson H. FTIR investigation of the interaction of tumor cells treated with caffeic acid and chlorogenic acid. Vib. Spectrosc. 2000, 24: 225-231.
[32] Morita S, Shinzawa H, Noda I, et al. Effect of band position shift on moving-window two-dimensional correlation spectroscopy. J. Mol. Struct. 2006, 799: 16-22.
[33] Morita S, Shinzawa H, Noda I, et al. Perturbation-correlation moving-window two-dimensional correlation spectroscopy. Appl. Spectrosc. 2006, 60: 398-406.
[34] Watanabe A, Morita S, Ozaki Y. Temperature-dependent structural changes in hydrogen bonds in microcrystalline cellulose studied by infrared and near-infrared spectroscopy with perturbation-correlation moving-window two-dimensional correlation analysis. Appl. Spectrosc. 2006, 60: 611-618.
[35] Zhou Q, Sun S Q, Zhan D Q, et al. Point-point and point-line moving-window correlation spectroscopy and its applications. J. Mol. Struct. 2008, 883-884: 109-115.
[36] Barton F E, Himmelsbach D S, Duckworth J H, et al. Two-dimensional vibration spectroscopy: correlation of mid- and near-infrared regions. Appl. Spectrosc. 1992, 46: 420-429.
[37] Barton F E, Himmelsbach D S. Two-dimensional vibration spectroscopy II: correlation of the absorptions of lignins in the mid- and near-infrared. Appl. Spectrosc. 1993, 47: 1920-1925.
[38] Pielesz A, Wesleucha-Birczynska A, Freeman H S, et al. Characterizing of model direct dyes interactions with cotton cellulose via 1D and 2D raman spectroscopy. Cellulose 2005, 12: 497-506.
[39] Moore A A, Jacobson M L, Belabas N, et al. 2D correlation analysis of the continuum in single molecule surface enhanced raman spectroscopy. J. Am. Chem. Soc. 2005, 127: 7292-7293.
[40] Elmore D L, Dluhy R.βν-Correlation Analysis: A modified two-dimensional infrared correlation method for determining relative rates of intensity change. J. Phys. Chem. B. 2001, 105: 11377-11386.
[41] Morita S, Ozaki Y, Noda I. Global phase angle description of generalized two-dimensional correlation spectroscopy: 1. theory and its simulation for practical use. Appl. Spectrosc. 2001, 55: 1618-1621.
[42] Noda I. Kernel analysis for two-dimensional (2D) correlation spectroscopy. J. Mol. Struct. 2006, 799: 34-40.
[43] Czarnecki M A, Wu PY, Siesler H W. 2D FT-NIR and FT-IR correlation analysis of temperature-induced changes of nylon12. Chem. Phys. Lett. 1998, 83: 326-332.
[44] Wu P Y, Siesler H W. Two-dimensional correlation analysis of variable temperature Fourier-transform mid and near-infrared spectra of plyamide11. J. Mol. Struc. 2000, 521: 37-47.
[45] Wu P Y, Yang Y L, Siesler H W. Two-dimentional near-infrared correlation temperature studies of an amorphous Polyamide. Polymer. 2001, 42: 10181-10186.
[46] Amari T, Ozaki Y. Generalized two-dimensional attenuated total reflection/infrared and near-infrared correlation spectroscopy studies of real-time monitoring of the initial oligomerization of bis(hydroxyethyl terephthalate). Macromolecules 2002, 35: 8020-8028.
[47] Shen Y, Chen F E, Wu P Y, et al. A two-dimensional Raman spectroscopic study on the structural changes of a polythiophene film during the cooling process. J. Chem. Phys. 2003, 119: 11415-11419.
[48] Kim H, Kim S B, Kim, J K, et al. Phase behavior of a weakly interacting block copolymer by temperature-dependent FTIR spectroscopy. Macromolecules 2006, 39: 408-412.
[49] Xu Y, Wu P Y. A study of water dehydration in nylon 6 as a function of temperature using two-dimensional (2D) correlation near-infrared (NIR) analysis. J. Mol. Struct. 2007, 833: 145-149.
[50] Wan L S, Huang X J, Xu Z K. Diffusion and structure of water in polymers containing N-vinyl-2-pyrrolidone. J. Phys. Chem. B. 2007, 111: 922-928.
[51] Thomas M, Richardson H. Two-dimensional FT-IR correlation analysis of the phase transitions in a liquid crystal, 4X-n-octyl-4-cyanobiphenyl (8CB). Vib. Spectrosc. 2000, 24: 137-146.
[52] Popescu M C, Filip D, Vasile C, et al. Characterization by Fourier transform infrared spectroscopy (FT-IR) and 2D IR correlation spectroscopy of PAMAM dendrimer. J. Phys. Chem. B. 2006, 110: 14198-14211.
[53] Jung Y M, Shin H S, Czarnik-Matusewicz B, et al. Characterization of transition temperatures of a Langmuir-Blodgett film of poly (tert-butyl methacrylate) by two-dimensional correlation spectroscopy and principal component analysis. Appl. Spectrosc. 2002, 56: 1568-1574.
[54] Azizan S, Shibata K, Matsuda T, et al. Surface phase transition of C12E1 at the air/water interface: a study by dynamic surface tension, external RA FT-IR, and 2D IR correlation methods. J. Phys. Chem. B. 2006, 110: 17034-17042.
[55] Sallach R, Wei M, Biswas N, et al. Micelle density regulated by a reversible switch of protein secondary structure. J. Am. Chem. Soc. 2006, 128: 12014-12019.
[56] Ashton L, Czarniz-Matusewicz B, Blanch E W. Application of two-dimensional correlation analysis to Raman optical activity. J. Mol. Struct. 2006, 799: 61-71.
[57] Yan Y B, Zhang J, He H W, et al. Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy. Biophys. J. 2006, 90: 2525-2533.
[58] Wang G, Gao Y, Geng M L. Generalized two-dimensional heterocorrelation analysis of spectrally resolved and temporally resolved fluorescence of the 8-anilino-1-naphthalenesulfonate-apomyoglobin complex with pH perturbation. J. Phys. Chem. B. 2006, 110: 8506-8512.
[59] Zhang J, He H W, Wang Q, et al. Sequential events in ribonuclease A thermal unfolding characterized by two-dimensional infrared correlation spectroscopy. Protein Pept. Lett. 2006, 13: 33-40.
[60] Otsuka M, Fukui Y, Otsuka K, et al. Comparative evaluation of bioactivity change of crystalline trypsin during compression by chemoinformatics and 2D Fourier-transform infrared spectroscopy. Analyst 2006, 131: 1116-1121.
[61] Lehmann N, Alexiev U, Fahmy K. Linkage between the intramembrane H-bond network around aspartic acid 83 and the cytosolic environment of helix 8 in photoactivated rhodopsin. J. Mol. Biol. 2007, 366: 1129-1141.
[62] Hu B W, Zhou P, Noda I, et al. An NMR approach applicable to biomolecular structure characterization. Anal. Chem. 2005, 77: 7534-7538.
[63] Watanabe A, Morita S, Ozaki Y. Study on temperature-dependent changes in hydrogen bonds in cellulose Iβby infrared spectroscopy with perturbation-correlation moving-window two-dimensional correlation spectroscopy. Biomacromolecules 2006, 7: 3164-3170.
[64] Verma D, Katti K, Katti D. Nature of water in nacre: A 2D Fourier transform infrared spectroscopic study. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2007, 67: 784-788.
[65] Yakimets I, Paes S S, Wellner N, et al. Effect of water content on the structural reorganization and elastic properties of biopolymer films: a comparative study. Biomacromolecules 2007, 8: 1710-1722.
[66] DeGelder J, DeGussem K, Vandenabeele P, et al. Methods for extracting biochemical information from bacterial Raman spectra: An explorative study on Cupriavidus metallidurans. Anal. Chim. Acta 2007, 585: 234-240.
[67] Yu L, Sun SQ, Fan KF, et al. Research on processing medicinal herbs with multi-steps infrared macro-fingerprint method. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2005, 62: 22-29.
[68] Xu C H, Sun S Q, Guo C Q, et al. Multi-steps infrared macro-fingerprint analysis for thermal processing of Fructus viticis. Vib. Spectrosc. 2006, 41: 118-125.
[69] Wu Y W, Sun S Q, Zhou Q, et al. Fourier transform mid-infrared (MIR) and near-infrared (NIR) spectroscopy for rapid quality assessment of Chinese medicine preparation Honghua Oil. J. Pharm. Biomed. Anal. 2008, 46: 498-504.
[70] Czarnik-Matusewicz B, Murayama K, Tsenkova R, et al. Analysis of near-infrared spectra of complicated biological fluids by two-dimensional correlation spectroscopy: Protein and fat concentration-dependent spectral changes of milk. Appl. Spectrosc. 1999, 53: 1582-1594.
[71] Liu Y L, Barton F E, Lyon B G, et al. Two-dimensional correlation analysis of visible/near-infrared spectral intensity variations of chicken breasts with various chilled and frozen storages. J. Agric. Food Chem. 2004, 52: 505-510.
[72] Sun S Q, Li C W, Wei J P, et al. Discrimination of chinese sauce liquor using FT-IR and two-dimensional correlation IR spectroscopy. J. Mol. Struct. 2006, 799: 72-76.
[73] Zhou Q, Sun S Q, Yu L, et al. Sequential changes of main components in different kinds of milk powders using two-dimensional infrared correlation analysis. J. Mol. Struct. 2006, 799: 77-84.
[74] Kizil R, Irudayaraj J. Rapid evaluation and discrimination of gamma-irradiated carbohydrates using FT-Raman spectroscopy and canonical discriminant analysis. J. Sci. Food Agric. 2003, 87: 1244-1251.
[75] Wynne L, Clark S, Adams M J, et al. Compositional dynamics of a commercial wine fermentation using two-dimensional FTIR correlation analysis. Vib. Spectrosc. 2007, 44: 394-400.
[76] Nagai N, Yamaguchi Y, Saito R, et al. IR and EPR study of the Na ion-limplanted SiO2/Si system. Appl. Spectrosc. 2001, 55: 1207-1213.
[77] Choi H C, Jung Y M, Noda I, et al. A study of the mechanism of the electrochemical reaction of lithium with CoO by two-dimensional soft X-ray absorption spectroscopy (2D XAS), 2D Raman, and 2D heterospectral XAS-Raman correlation analysis. J. Phys. Chem. B 2003, 107: 5806-5811.
[78] Czarnik-Matusewicz B, Pilorz S, Hawranek J P. Temperature-dependent water structural transitions examined by near-IR and mid-IR spectra analyzed by multivariate curve resolution and two-dimensional correlation spectroscopy. Anal. Chim. Acta 2005, 544: 15-25.
[79] Sasaki T, Itoh J, Horiguchi Y, et al. Quantitative determination of corrosion products and adsorbed water on copper in humid air containing SO2 by IR-RAS measurements. Corros. Sci. 2006, 48: 4339-4351.
[80] Noda I, Liu Y L, Ozaki Y, et al. Two-dimensional fourier-transform near-infrared correlation spectroscopy studies of temperature-dependent spectral variations of oleyl alcohol. J. Phys.
[81] Noda I, Marcott C. Two-dimensional raman (2D Raman) correlation spectroscopy study of non-oxidative photodegradation ofβ-carotenet. J. Phys. Chem. A 2002, 106: 3371-3376.
[82] Zhong Z, Wang G, Geng M L. Probing strong adsorption of individual solute molecules at solid/liquid interfaces with model-based statistical two-dimensional correlation analysis. J. Mol. Struct. 2006, 799: 204-210.
[83] Nakashima K, Fukuma H, Ozaki Y, et al. Two-dimensional correlation fluorescence spectroscopy V: Polarization perturbation as a new technique to induce intensity changes in fluorescence spectra. J. Mol. Struct. 2006, 799: 52-55.
[84] Chen Y, Zhang H, Huang C, et al. Synthesis and two-dimensional correlation infrared spectroscopy study of an organic–inorganic hybrid compound constructed by [Mo5P2O23] and Cu-imidazole components. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2006, 63: 536-540.
[85] Popescu M C, Zanoaga M, Mamunya Y, et al. Two dimensional infrared correlation spectroscopy studies of wood-plastic composites with a copolyamide as matrix. J. Optoelectron. Adv. Mater. 2007, 9: 923-930.
[86] Hua R, Sun S Q, Zhou Q, et al. Discrimination of Fritillary according to geographical origin with Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. J. Pharm. Biomed. Anal. 2003, 33: 199-209.
[87] Liu Y, Wang J Q, Liu S H, et al. Two-dimensional correlation infrared spectroscopy applied to analyzing and identifying the Radix paeoniae Alba medicinal materials. J. Mol. Struct. 2008, 883-884: 137-141.
[88] Zhou Q, Sun S Q, Zuo L. Study on traditional Chinese medicine 'Qing Kai Ling' injections from different manufactures by 2D IR correlation spectroscopy. Vib. Spectrosc. 2004, 36: 207-212.
[89] Wu Y W, Sun S Q, Zhou Q, et al. Volatility-dependent 2D IR correlation analysis of traditional Chinese medicine 'Red Flower Oil' preparation from different manufacturers. J. Mol. Struct. 2008, 882: 107-115.
[90] Li Y M, Sun S Q, Zhou Q, et al. Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy. Vib. Spectrosc. 2004, 36: 227-232.
[91] Lu G H, Zhou Q, Sun S Q, et al. Differentiation of Asian ginseng, American ginseng and Notoginseng by Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy. J. Mol. Struct. 2008, 883-884: 91-98.
[92] Liu D, Li Y G, Xu H, et al. Differentiation of the root of cultivated ginseng, mountain cultivated ginseng and mountain wild ginseng using FT-IR and two-dimensional correlation IR spectroscopy. J. Mol. Struct. 2008, 883-884: 228-235.
[93]詹达琦,张晓明,孙素琴.基于小波变换的二维红外相关光谱鉴别人参的生长年限.光谱学与光谱分析. 2007, 27: 1497-1501.
[94] Liu H X, Sun S Q, Lv G H, et al. Discrimination of extracted lipophilic constituents of Angelica with multi-steps infrared macro-fingerprint method. Vib. Spectrosc. 2006, 40: 202-208.
[95] Liu H X, Sun S Q, Lv G H, et al. Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2006, 64: 321-326.
[96] Liu H X, Zhou Q, Sun S Q, et al. Discrimination of different Chrysanthemums with Fourier transform infrared spectroscopy. J. Mol. Struct. 2008, 883-884: 38-47.
[97]周晶,孙建云,徐胜艳,等.八角茴香与其伪品莽草的红外光谱三级鉴定研究.光谱学与光谱分析. 2008, 28: 2864-2867.
[98]刘军,周群,孙素琴.柴胡及其伪品大叶柴胡红外光谱的分析与鉴定.光谱学与光谱分析. 2008, 28: 107-108.
[99]阿依古丽,周群,董晓鸥,等.甘草真伪品的FTIR光谱法鉴别研究.光谱学与光谱分析. 2006, 26: 1238-1241.
[100]金哲雄,徐胜艳,孙素琴,等.刺五加不同部位的红外光谱分析与鉴定.光谱学与光谱分析. 2008, 28: 2859-2863.
[101]徐荣,孙素琴,刘友刚,等.红外光谱法对肉苁蓉径向不同部位的分析与评价.分析化学. 2009, 37: 221-226.