植物激素在百合切花保鲜中的应用及其机理研究
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摘要
本文旨在系统研究植物五大激素,即乙烯、赤霉素、细胞分裂素、生长素和脱落酸在百合切花中的保鲜效应,探讨其对切花保鲜的生理生化反应,明确影响切花衰老的主要因子。主要研究内容与结果如下:
(1)以‘黄天霸’为材料,在含不同乙烯抑制剂(STS和亚精胺)配方预处理18h后,常温或4℃冷藏后瓶插,测定各项指标。结果表明:两者均具保鲜效果,能增加鲜重,降低膜质过氧化程度,显著延长切花瓶插寿命。低温贮藏切花对乙烯抑制剂敏感度提高,保鲜效果较常温显著。
(2)选择不同浓度GA_3、6-BA、ABA、IAA对‘索邦’百合进行预处理18h后瓶插,观察测定其形态和生理指标。总体而言,适当浓度的GA_3保绿效果明显,可延长切花瓶插寿命,但对其它形态观赏价值影响并不显著;6-BA的整体保鲜效果不理想,适当浓度6-BA处理虽可一定程度上延长瓶插寿命,但与仅加蔗糖与杀菌剂的基本配方相比,差异并不显著。适当浓度GA_3和6-BA处理可遏制MDA产生,提高SOD活性,保护细胞膜的完整性,但明显抑制花青素的合成。外源ABA处理效果具双重性,低浓度可提高切花观赏质量,但过高浓度会导致叶片黄化;较低浓度IAA处理对切花瓶插质量影响不显著,但高浓度则明显促进切花衰老。
(3)植物激素在切花衰老过程中生理效应各不相同,其保鲜应用价值也存在差异。除IAA外,适当浓度的GA_3、6-BA和ABA能延长切花寿命,延缓切花衰老,故具有一定保鲜应用价值,但过高浓度有抑制作用。
The thesis aimed to study systematicly the preservation affects of five plant hormones, namely ethylene, gibberellin, cytokinin, auxin and abscisic acid on cut lily flowers. It explored different physiological and biochemical reactions of flower preservation and cleared the main factors of senescence in this experiment. The results are as follows:
(1) OT hybrids lily‘Mantissa’were used in the study. The pulsing treatments (STS and spermidine) were carried out for 18 hours at room temperature. These results showed that the two ethylene inhibitors both have certain quality of products, which can increase fresh weight, reduce lipid peroxidation, and extend the vase life significantly. Cold storage on ethylene inhibitors improved sensitivity, with more significant effect on preservation than temperature.
(2) Different concentration of GA_3, 6-BA, ABA, and IAA were selected on the 'Sorbonne' Lily after 18h pretreatment to observe the morphological and physiological indicators. Overall, the appropriate concentration of GA_3 treatment had good effect on preventing the leaves from yellowing, and extending the life in the vase to some extent, but it didn’t affect the ornamental value significantly. The preservation of 6-BA pretreatments was unsatisfactory. The appropriate concentration of 6-BA treatment could extend cut vase life to some extent, but comparing with the basic formula which only adding sugar and fungicide, the difference was not significant. Appropriate concentration of GA_3 and 6-BA treatment could curb the production of MDA, and increase SOD activity to protect the integrity of cell membrane, but it inhibited the synthesis of anthocyanin significantly. ABA pretreatments had a duality that low concentration of ABA could improve the viewing quality of cut flowers, while high concentrations resulted in yellow leaves; lower concentrations of IAA treatment on the vase and other related indicators of quality were not significant; however, higher concentrations of IAA treatment inhibited the senescence.
(3) Plant hormones in the process of senescence had different physiological effects, so were their preservation value.GA_3, 6-BA and ABA, were keeping a certain value, while the IAA was not suitable for the fresh cut flowers. It was mainly due to the appropriate concentrations of GA_3, 6-BA and ABA can prolong life of cut flowers, and delay of senescence, but high concentrations were inhibitory.
(1)以‘黄天霸’为材料,在含不同乙烯抑制剂(STS和亚精胺)配方预处理18h后,常温或4℃冷藏后瓶插,测定各项指标。结果表明:两者均具保鲜效果,能增加鲜重,降低膜质过氧化程度,显著延长切花瓶插寿命。低温贮藏切花对乙烯抑制剂敏感度提高,保鲜效果较常温显著。
(2)选择不同浓度GA_3、6-BA、ABA、IAA对‘索邦’百合进行预处理18h后瓶插,观察测定其形态和生理指标。总体而言,适当浓度的GA_3保绿效果明显,可延长切花瓶插寿命,但对其它形态观赏价值影响并不显著;6-BA的整体保鲜效果不理想,适当浓度6-BA处理虽可一定程度上延长瓶插寿命,但与仅加蔗糖与杀菌剂的基本配方相比,差异并不显著。适当浓度GA_3和6-BA处理可遏制MDA产生,提高SOD活性,保护细胞膜的完整性,但明显抑制花青素的合成。外源ABA处理效果具双重性,低浓度可提高切花观赏质量,但过高浓度会导致叶片黄化;较低浓度IAA处理对切花瓶插质量影响不显著,但高浓度则明显促进切花衰老。
(3)植物激素在切花衰老过程中生理效应各不相同,其保鲜应用价值也存在差异。除IAA外,适当浓度的GA_3、6-BA和ABA能延长切花寿命,延缓切花衰老,故具有一定保鲜应用价值,但过高浓度有抑制作用。
The thesis aimed to study systematicly the preservation affects of five plant hormones, namely ethylene, gibberellin, cytokinin, auxin and abscisic acid on cut lily flowers. It explored different physiological and biochemical reactions of flower preservation and cleared the main factors of senescence in this experiment. The results are as follows:
(1) OT hybrids lily‘Mantissa’were used in the study. The pulsing treatments (STS and spermidine) were carried out for 18 hours at room temperature. These results showed that the two ethylene inhibitors both have certain quality of products, which can increase fresh weight, reduce lipid peroxidation, and extend the vase life significantly. Cold storage on ethylene inhibitors improved sensitivity, with more significant effect on preservation than temperature.
(2) Different concentration of GA_3, 6-BA, ABA, and IAA were selected on the 'Sorbonne' Lily after 18h pretreatment to observe the morphological and physiological indicators. Overall, the appropriate concentration of GA_3 treatment had good effect on preventing the leaves from yellowing, and extending the life in the vase to some extent, but it didn’t affect the ornamental value significantly. The preservation of 6-BA pretreatments was unsatisfactory. The appropriate concentration of 6-BA treatment could extend cut vase life to some extent, but comparing with the basic formula which only adding sugar and fungicide, the difference was not significant. Appropriate concentration of GA_3 and 6-BA treatment could curb the production of MDA, and increase SOD activity to protect the integrity of cell membrane, but it inhibited the synthesis of anthocyanin significantly. ABA pretreatments had a duality that low concentration of ABA could improve the viewing quality of cut flowers, while high concentrations resulted in yellow leaves; lower concentrations of IAA treatment on the vase and other related indicators of quality were not significant; however, higher concentrations of IAA treatment inhibited the senescence.
(3) Plant hormones in the process of senescence had different physiological effects, so were their preservation value.GA_3, 6-BA and ABA, were keeping a certain value, while the IAA was not suitable for the fresh cut flowers. It was mainly due to the appropriate concentrations of GA_3, 6-BA and ABA can prolong life of cut flowers, and delay of senescence, but high concentrations were inhibitory.
引文
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