iNOS在口腔粘膜下纤维化、扁平苔藓、白斑和鳞癌组织中的表达差异研究
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摘要
目的通过研究诱导型一氧化氮合酶(inducible nitric oxidesynthase,iNOS)在正常口腔粘膜(Normal buccal mucous,NBM)、口腔粘膜下纤维化(oral submucous fibrosis,OSF)、口腔扁平苔藓(orallichen planus,OLP)、口腔白斑(oral leukoplakia,OLK)和口腔鳞癌(oral squamous cell carcinoma,OSCC)组织中的表达差异,探讨OSF癌变潜能大小及iNOS在其癌变过程中的可能作用。
方法应用免疫组织化学染色法检测10例正常口腔粘膜,30例OSF(早、中、晚各10例),15例OLP,15例OLK和15例OSCC组织中iNOS的表达情况,比较iNOS在5组间的阳性表达率和表达强度,并进行统计学分析。
结果①iNOS在正常口腔粘膜上皮中呈阴性表达,在OSF、OLP、OLK、OSCC中呈明显表达,棕黄色阳性颗粒主要位于上皮棘细胞,基底细胞胞浆胞膜上。
②iNOS在OSF、OLP、OLK、OSCC中的阳性表达率与表达强度与NBM组之间的差别具有显著意义(P＜0.05),而OSF与OLP,OLK比较差异无显著性(P＞0.05),OSF早中晚三组间无显著性差异(P＞0.05)。iNOS在OSF中的阳性表达率与OSCC组无显著性差异(P＞0.05),但表达强度低于OSCC组,差异具有显著性(P＜0.05)。
③OLP、OSF、OLK的癌变率与iNOS的表达强度呈正相关关系(P＜0.05)。
结论①iNOS在OSF早、中、晚三期中的高表达提示其三组上皮细胞均具有癌变的潜能,该潜能可能与临床病理分期无密切关系。
②iNOS参与了OSF等口腔粘膜癌前病变的发生发展,其癌变潜能可能与iNOS的表达有密切关系,其顺序是:OLP,OSF,OLK,而iNOS在OSCC表达强度最强。
Object: To investigate the different expression of inducible nitric oxide synthase(iNOS) among normal buccal mucous (NBM), oral submucous fibrosis(OSF), oral lichen planus(OLP), oral leukoplakia(OLK) and oral squamous cell carcinoma(OSCC), so as to evaluate the carcinogenic ability of OSF and discuss the role of iNOS in the carcinogenesis of OSF.
Method: The expression of iNOS was examined by immunohistochemical SP method in 30 cases of OSF(comprises of 10 initial, 10 medium-term and 10 advanced stage cases), 15 cases of OLP, 15 cases of OLK, 15 cases of OSCC and 10 cases of NBM. The different positive cell rates and expression intensity of iNOS in the five groups were compared by statistics analysis.
Results:
1. NBM showed negative positive expression of iNOS. There were obvious expressions of iNOS in OSF, OLP, OLK and OSCC. Positive brown staining was mostly presented in plasm and membrane of basal and spinous cells.
2. The differences of positive cell rates and expression intensity of iNOS between OSF, OLP, OLK, OSCC and NBM were statistically significant (P<0.05), but there were no significant differences between OSF and OLP, OSF and OLK.(P>0.05). There were no significant differences among the three stages in OSF(P>0.05). The difference of positive cell rates of iNOS between OSF and OSCC was no statistically significant (P<0.05), but the expression intensity of OSF is lower than that of OSCC, the difference is statistically significant(P<0.05).
3. The carcinogenesis rates of OLP, OSF, OLK were related to the expression intensity of iNOS (P<0.05).
Conclusion:
1. The overexpression of iNOS in initial, medium-term and advanced stages of OSF possibly indicates the malignant potential of epithelia cells of three groups. There is possibly no correlation between malignant potential and the clinical pathological stage.
2. The iNOS may involve in initiation and development of several oral precancerous lesions including OSF, and the malignant potential may have close relationship with the expression intensity of iNOS. The malignant potential order is OLP, OSF, OLK, the most intense expression of iNOS can be seen in OSCC.
方法应用免疫组织化学染色法检测10例正常口腔粘膜,30例OSF(早、中、晚各10例),15例OLP,15例OLK和15例OSCC组织中iNOS的表达情况,比较iNOS在5组间的阳性表达率和表达强度,并进行统计学分析。
结果①iNOS在正常口腔粘膜上皮中呈阴性表达,在OSF、OLP、OLK、OSCC中呈明显表达,棕黄色阳性颗粒主要位于上皮棘细胞,基底细胞胞浆胞膜上。
②iNOS在OSF、OLP、OLK、OSCC中的阳性表达率与表达强度与NBM组之间的差别具有显著意义(P＜0.05),而OSF与OLP,OLK比较差异无显著性(P＞0.05),OSF早中晚三组间无显著性差异(P＞0.05)。iNOS在OSF中的阳性表达率与OSCC组无显著性差异(P＞0.05),但表达强度低于OSCC组,差异具有显著性(P＜0.05)。
③OLP、OSF、OLK的癌变率与iNOS的表达强度呈正相关关系(P＜0.05)。
结论①iNOS在OSF早、中、晚三期中的高表达提示其三组上皮细胞均具有癌变的潜能,该潜能可能与临床病理分期无密切关系。
②iNOS参与了OSF等口腔粘膜癌前病变的发生发展,其癌变潜能可能与iNOS的表达有密切关系,其顺序是:OLP,OSF,OLK,而iNOS在OSCC表达强度最强。
Object: To investigate the different expression of inducible nitric oxide synthase(iNOS) among normal buccal mucous (NBM), oral submucous fibrosis(OSF), oral lichen planus(OLP), oral leukoplakia(OLK) and oral squamous cell carcinoma(OSCC), so as to evaluate the carcinogenic ability of OSF and discuss the role of iNOS in the carcinogenesis of OSF.
Method: The expression of iNOS was examined by immunohistochemical SP method in 30 cases of OSF(comprises of 10 initial, 10 medium-term and 10 advanced stage cases), 15 cases of OLP, 15 cases of OLK, 15 cases of OSCC and 10 cases of NBM. The different positive cell rates and expression intensity of iNOS in the five groups were compared by statistics analysis.
Results:
1. NBM showed negative positive expression of iNOS. There were obvious expressions of iNOS in OSF, OLP, OLK and OSCC. Positive brown staining was mostly presented in plasm and membrane of basal and spinous cells.
2. The differences of positive cell rates and expression intensity of iNOS between OSF, OLP, OLK, OSCC and NBM were statistically significant (P<0.05), but there were no significant differences between OSF and OLP, OSF and OLK.(P>0.05). There were no significant differences among the three stages in OSF(P>0.05). The difference of positive cell rates of iNOS between OSF and OSCC was no statistically significant (P<0.05), but the expression intensity of OSF is lower than that of OSCC, the difference is statistically significant(P<0.05).
3. The carcinogenesis rates of OLP, OSF, OLK were related to the expression intensity of iNOS (P<0.05).
Conclusion:
1. The overexpression of iNOS in initial, medium-term and advanced stages of OSF possibly indicates the malignant potential of epithelia cells of three groups. There is possibly no correlation between malignant potential and the clinical pathological stage.
2. The iNOS may involve in initiation and development of several oral precancerous lesions including OSF, and the malignant potential may have close relationship with the expression intensity of iNOS. The malignant potential order is OLP, OSF, OLK, the most intense expression of iNOS can be seen in OSCC.
引文
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