福建黄兔微卫星多态性及其与生长性状的关联分析
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摘要
选用8个微卫星标记与福建黄兔的基因组进行PCR扩增，扩增结果用聚丙烯酰胺凝胶电泳进行分型，分析微卫星标记的多态性及其与生长性状的关联。结果表明：
在福建黄兔三个品系6个群体的8个微卫星座位共检测到49个等位基因，每个座位平均6.1，最多为7个，最少为5个。其中，22个等位基因为6个群体所共有。所选用的8个微卫星座位的杂合度在0.5904～0.7267之间，平均为0.6857。多态信息含量在0.5723～0.7222之间，平均为0.6622。
计算了6个群体的遗传距离，并构建了系统聚类图。遗传距离最近的是连江与连城群体，为0.1206；遗传距离最远的是连城与上杭群体，为0.4446。聚为三类：黄毛品系连江与连城群体聚为一类；黑毛品系中上杭与德化群体先聚为一类，而后再与大田群体聚到一起；白毛品系聚为一类。
通过最小二乘分析微卫星遗传标记与体重、体尺性状的相关。结果显示：微卫星座位Sol33 209bp等位基因可能对1月龄体重有较强的正面影响；座位Satl2 125bp和座位Sol33201bp等2个等位基因可能对3月龄体重有较强的正面影响；座位Sol33 213bp等位基因可能对8月龄体重有负面影响；微卫星座位Sol03中如果242bp等位基因的正面影响存在，240bp等位基因可能对8月龄体重有较强的负面影响；座位Sol44 217bp、座位Sol33 213bp以及座位Sol03 236bp等3个等位基因可能对体长性状有正面影响；座位Sol44 227bp、座位Sol33 213bp以及座位Sol03 244bp等3个位基因可能对胸围性状有正面影响；座位Sol44203bp等位基因可能对胸围性状有负面影响。这为进一步QTL定位提供理论依据。
Eight microsatellite markers were chosed to analyze correlation between polymorphims of microsatellite markers, body size and body weight of Fujian yellow rabbit through PCR-PAGE method. The results indicated:
There were 49 alleles in eight microsatellite loci, and each locus had 6.1. The most was 7 and the least was 5.There are 21 alleles in common in six populations.The average heterozygosit(H) was 0.6857,ranging 0.5904 from 0.7267.The average polymorphism information content(PIC) was 0.6622,ranging from 0.5723 to 0.7222.
The genetic distance between population and the dendrogram were calculated.The genetic distances between lianjiang populations and liancheng populations was the nearest(0.1206).The genetic distances between liancheng population and shanghang population was the furthest (0.4446).The cluster analysis results showed that 6 populations were divided into three categories:1) lianjiang and liancheng,2) shanghang.dehua and datian,3)shouning.
On the other side,least square means were analyzed. Many genotypes of microsatellites were significant correlations with body size and body weight.The allele 209bp of Sol33 had a positive effect on first month' weight.The allele 125bp of Satl2 and allele 201bp of Sol33 had a strong positive impact on 1-month-old weight. The allele 213bp of Sol33 may have a negative impact on the 8-month-old weight. If allele 242bp of Sol03 had a positive impact, the allele 240bp had a strong negative impact on the 8-month-old weight. The allele 217bp of Sol44, allele 213bp of Sol33 and allele 236bp of Sol03 had a positive impact on stem length.The allele 213bp of Sol33, allele 170bp of Sol30 and allele 244bp of Sol03 may have a positive impact on chest circumference. The allele 203bp of Sol44 may have a negative impact on chest circumference.This will provide a theoretical basis for further QTL.
在福建黄兔三个品系6个群体的8个微卫星座位共检测到49个等位基因，每个座位平均6.1，最多为7个，最少为5个。其中，22个等位基因为6个群体所共有。所选用的8个微卫星座位的杂合度在0.5904～0.7267之间，平均为0.6857。多态信息含量在0.5723～0.7222之间，平均为0.6622。
计算了6个群体的遗传距离，并构建了系统聚类图。遗传距离最近的是连江与连城群体，为0.1206；遗传距离最远的是连城与上杭群体，为0.4446。聚为三类：黄毛品系连江与连城群体聚为一类；黑毛品系中上杭与德化群体先聚为一类，而后再与大田群体聚到一起；白毛品系聚为一类。
通过最小二乘分析微卫星遗传标记与体重、体尺性状的相关。结果显示：微卫星座位Sol33 209bp等位基因可能对1月龄体重有较强的正面影响；座位Satl2 125bp和座位Sol33201bp等2个等位基因可能对3月龄体重有较强的正面影响；座位Sol33 213bp等位基因可能对8月龄体重有负面影响；微卫星座位Sol03中如果242bp等位基因的正面影响存在，240bp等位基因可能对8月龄体重有较强的负面影响；座位Sol44 217bp、座位Sol33 213bp以及座位Sol03 236bp等3个等位基因可能对体长性状有正面影响；座位Sol44 227bp、座位Sol33 213bp以及座位Sol03 244bp等3个位基因可能对胸围性状有正面影响；座位Sol44203bp等位基因可能对胸围性状有负面影响。这为进一步QTL定位提供理论依据。
Eight microsatellite markers were chosed to analyze correlation between polymorphims of microsatellite markers, body size and body weight of Fujian yellow rabbit through PCR-PAGE method. The results indicated:
There were 49 alleles in eight microsatellite loci, and each locus had 6.1. The most was 7 and the least was 5.There are 21 alleles in common in six populations.The average heterozygosit(H) was 0.6857,ranging 0.5904 from 0.7267.The average polymorphism information content(PIC) was 0.6622,ranging from 0.5723 to 0.7222.
The genetic distance between population and the dendrogram were calculated.The genetic distances between lianjiang populations and liancheng populations was the nearest(0.1206).The genetic distances between liancheng population and shanghang population was the furthest (0.4446).The cluster analysis results showed that 6 populations were divided into three categories:1) lianjiang and liancheng,2) shanghang.dehua and datian,3)shouning.
On the other side,least square means were analyzed. Many genotypes of microsatellites were significant correlations with body size and body weight.The allele 209bp of Sol33 had a positive effect on first month' weight.The allele 125bp of Satl2 and allele 201bp of Sol33 had a strong positive impact on 1-month-old weight. The allele 213bp of Sol33 may have a negative impact on the 8-month-old weight. If allele 242bp of Sol03 had a positive impact, the allele 240bp had a strong negative impact on the 8-month-old weight. The allele 217bp of Sol44, allele 213bp of Sol33 and allele 236bp of Sol03 had a positive impact on stem length.The allele 213bp of Sol33, allele 170bp of Sol30 and allele 244bp of Sol03 may have a positive impact on chest circumference. The allele 203bp of Sol44 may have a negative impact on chest circumference.This will provide a theoretical basis for further QTL.
引文
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[8] Bandoc O L, Flor M C G T, Rehollos S D N, et al. Variations in karyotypic characteristics of difference breed group in water buffaloes (bubalus bubalis) [J]. Asian-Aust J Anim Sci., 2002, 15(3): 321～325.
[9] 刘莉，谢芳，成荣等．泰和乌骨鸡染色体G-带、Ag-NORs与生产性能关系[J]．扬州大学学报(农业与生命科学版)，2003，24(3)：23～28．
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[11] 王偕根，陈国宏，张学余等．鹿苑鸡核型及其与生产性能的相关性研究[J]．中国家禽，2003，25(6)．
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