新西兰兔血液学参数的研究
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摘要
3月龄左右新西兰兔是最常用的一种实验用兔。其主要用于免疫学、各种生物制品的鉴定、生殖生理和避孕、微生物学、心血管和冠心病、急性动物实验、胆固醇代谢和动脉粥样硬化症、遗传性疾病和生理代谢失常、生理教学等方面的研究。这些研究都与新西兰兔血液学参数有着密切的关系。血液学指标受种属、年龄、生活环境、应激、检测仪器等多种因素影响,而且目前国内外关于新西兰兔血液学参数的报道多见于为自身实验设计需要的血液测定指标,缺乏具有实验室通用性的新西兰兔的血液学参数。
本研究以3月龄新西兰兔100只(雌雄各50只)为研究对象,使用全自动血细胞分析仪对血常规中白细胞(WBC),嗜中性细胞百分率(NEU%),淋巴细胞百分率(LYM%),单核细胞百分率(MONO%),嗜酸性细胞百分率(EOS%),嗜碱性细胞百分率(BASO%),红细胞(RBC),血红蛋白(HGB),红细胞比积(HCT),平均红细胞体积(MCV),平均红细胞血红蛋白含量(MCH),平均红细胞血红蛋白浓度(MCHC),血小板(PLT),血小板压积(PCT),平均血小板体积(MPV),血小板体积分布宽度(PDW),红细胞分布宽度(RDW)十七项指标进行测定和分析；使用全自动生化分析仪对血生化中总蛋白(TP),白蛋白(ALB),球蛋白(GLO),白球比(A/G),丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(ALP),肌酸激酶(CK),血糖(GLU),血尿素氮(BUN),肌酐(Cr),钙(Ca),磷(P),总胆固醇(TC),甘油三酯(TG)十五项指标进行测定和分析。结果表明：3月龄新西兰兔雌雄间血常规指标中平均红细胞血红蛋白浓度(MCHC)差异显著(P<0.05),其它十六项指标参数差异不显著(P>0.05)；血生化指标中总蛋白(TP),球蛋白(GLO)、肌酐(Cr)和磷(P)四项指标差异极显著(P<0.01),碱性磷酸酶(ALP)和血尿素氮(BUN)两项指标差异显著(P<0.05),其它指标参数间差异不显著(P>0.05)。
本研究测定的3月龄不同性别间新西兰兔血常规及生化参数,可为病理、毒理学、发病机理疾病诊断等研究提供正常值的参考。
New Zealand White rabbits is one kind of laboratory rabbits, especially at 3-month-old. It mainly applied to the study of immunology, ingredient identification, reproductive physiology and contraception, microbiology, cardiovascular and cardiopathy, acute zoopery, cholesterol metabolism and atherosclerosis, heredopathia and disorder of physiological metabolism and experimental teaching, which have close relation with the research of blood parameters in New Zealand White rabbits. Nowadays, the reports about the blood parameters of New Zealand White rabbits are mainly due to the need of self-designed experiments. On account of the influences of species, age, habitat, stress and detecting instruments in the measurement of blood parameters, the blood parameters determinations can't be regarded as the correct parameters in the experiments using New Zealand White rabbits.
In this study, one hundred three-month-old New Zealand White rabbits were taken as research object, which included 50 females and 50 males. We used automatic blood routine analyzer to determine and analyze these seventeen indicators, such as white blood cell(WBC), the percentage of neutrophil leucocyte(NEU%), the percentage of lymphocyte(LYM%), the percentage of monocyte(MONO%), the percentage of eosinophil leucoeyte(EOS%), the percentage of basophil leucocyte(BASO%), red blood cell(RBC),haemoglobin (HGB), hematoerit(HCT), mean corpuscular volume(MCV), mean corpuscular hemoglobin content(MCH), mean corpuscular hemoglobin concentration(MCHC), platelet count(PLT), plateletocrit(PCT), mean platelet volume(MPV), platelet distribution width(PDW), red blood cell volume distribution width(RDW). We also used automatic serum biochemistry analyzer to determine and analyze the following indicators, such as total protein(TP), albumin(ALB), globulin(GLO), albumin/globulin(A/G), lanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), creatine kinase(CK), glucose(GLU), blood urea nitrogen(BUN), creatinine(Cr), calcium(Ca), phosphate(P), total cholesterol(TC), triglyceride(TG). It is concluded that a statistically significant difference was found for MCHC of blood routine in three-month-old New Zealand White rabbits(P<0.05), and another sixteen relative indicators of blood routine have no significant difference between groups (P>0.05). Besides, TP, GLO, Cr and P had highly significant difference (P<0.01), ALP and BUN have significant differences (P<0.05), between female and male by analyzing, otherwise another relative blood biochemistry indicators have no significant difference between five groups (P>0.05).
We determined the blood routine, blood biochemistry of different sexual three-month-old New Zealand White rabbits. The normal values of those indicators can provide references for pathematology, toxicology and disease diagnosis.
本研究以3月龄新西兰兔100只(雌雄各50只)为研究对象,使用全自动血细胞分析仪对血常规中白细胞(WBC),嗜中性细胞百分率(NEU%),淋巴细胞百分率(LYM%),单核细胞百分率(MONO%),嗜酸性细胞百分率(EOS%),嗜碱性细胞百分率(BASO%),红细胞(RBC),血红蛋白(HGB),红细胞比积(HCT),平均红细胞体积(MCV),平均红细胞血红蛋白含量(MCH),平均红细胞血红蛋白浓度(MCHC),血小板(PLT),血小板压积(PCT),平均血小板体积(MPV),血小板体积分布宽度(PDW),红细胞分布宽度(RDW)十七项指标进行测定和分析；使用全自动生化分析仪对血生化中总蛋白(TP),白蛋白(ALB),球蛋白(GLO),白球比(A/G),丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(ALP),肌酸激酶(CK),血糖(GLU),血尿素氮(BUN),肌酐(Cr),钙(Ca),磷(P),总胆固醇(TC),甘油三酯(TG)十五项指标进行测定和分析。结果表明：3月龄新西兰兔雌雄间血常规指标中平均红细胞血红蛋白浓度(MCHC)差异显著(P<0.05),其它十六项指标参数差异不显著(P>0.05)；血生化指标中总蛋白(TP),球蛋白(GLO)、肌酐(Cr)和磷(P)四项指标差异极显著(P<0.01),碱性磷酸酶(ALP)和血尿素氮(BUN)两项指标差异显著(P<0.05),其它指标参数间差异不显著(P>0.05)。
本研究测定的3月龄不同性别间新西兰兔血常规及生化参数,可为病理、毒理学、发病机理疾病诊断等研究提供正常值的参考。
New Zealand White rabbits is one kind of laboratory rabbits, especially at 3-month-old. It mainly applied to the study of immunology, ingredient identification, reproductive physiology and contraception, microbiology, cardiovascular and cardiopathy, acute zoopery, cholesterol metabolism and atherosclerosis, heredopathia and disorder of physiological metabolism and experimental teaching, which have close relation with the research of blood parameters in New Zealand White rabbits. Nowadays, the reports about the blood parameters of New Zealand White rabbits are mainly due to the need of self-designed experiments. On account of the influences of species, age, habitat, stress and detecting instruments in the measurement of blood parameters, the blood parameters determinations can't be regarded as the correct parameters in the experiments using New Zealand White rabbits.
In this study, one hundred three-month-old New Zealand White rabbits were taken as research object, which included 50 females and 50 males. We used automatic blood routine analyzer to determine and analyze these seventeen indicators, such as white blood cell(WBC), the percentage of neutrophil leucocyte(NEU%), the percentage of lymphocyte(LYM%), the percentage of monocyte(MONO%), the percentage of eosinophil leucoeyte(EOS%), the percentage of basophil leucocyte(BASO%), red blood cell(RBC),haemoglobin (HGB), hematoerit(HCT), mean corpuscular volume(MCV), mean corpuscular hemoglobin content(MCH), mean corpuscular hemoglobin concentration(MCHC), platelet count(PLT), plateletocrit(PCT), mean platelet volume(MPV), platelet distribution width(PDW), red blood cell volume distribution width(RDW). We also used automatic serum biochemistry analyzer to determine and analyze the following indicators, such as total protein(TP), albumin(ALB), globulin(GLO), albumin/globulin(A/G), lanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), creatine kinase(CK), glucose(GLU), blood urea nitrogen(BUN), creatinine(Cr), calcium(Ca), phosphate(P), total cholesterol(TC), triglyceride(TG). It is concluded that a statistically significant difference was found for MCHC of blood routine in three-month-old New Zealand White rabbits(P<0.05), and another sixteen relative indicators of blood routine have no significant difference between groups (P>0.05). Besides, TP, GLO, Cr and P had highly significant difference (P<0.01), ALP and BUN have significant differences (P<0.05), between female and male by analyzing, otherwise another relative blood biochemistry indicators have no significant difference between five groups (P>0.05).
We determined the blood routine, blood biochemistry of different sexual three-month-old New Zealand White rabbits. The normal values of those indicators can provide references for pathematology, toxicology and disease diagnosis.
引文
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