不同脂肪酸对朗德鹅填饲的效果及脂肪酸合成酶RFLP多态性与生产性能的相关研究
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摘要
本研究将14周龄、体重相近的健壮朗德鹅120只分成6组,每组20只,每只一个重复。玉米为主要填饲饲料;试验组在日填饲量的基础上添加一定量的脂肪酸:6%葵花籽油(葵花籽油组)、6%菜籽油(菜籽油组)、6%牛油(牛油组)、3%鹅油(鹅油Ⅰ组)、2%鹅油(鹅油Ⅱ组),对照组除不添加任何脂肪酸外,其他与试验组一致。试验结果:添加脂肪酸能影响填饲朗德鹅的产肝性能,其中以葵花籽油效果最好,肝重是对照组的1.37倍,差异显著(P＜0.05);牛油使填饲朗德鹅产肝性能下降。试验组对屠宰性能影响不如产肝性能影响明显,添加菜籽油使屠宰性能最好,活重、填饲期间增重、屠体重、半净膛重都与对照组差异显著(P＜0.05)。同样,腹脂沉积能力最强的也是菜籽油组,与对照组差异显著(P＜0.05),但是试验组之间差异不显著(P＞0.05)。
本研究还采用PCR-RFLP技术,分析了FAS基因启动子区在朗德鹅中的AfaⅠ酶切片段多态性分布。FAS基因启动子区具有3个AfaⅠ酶切位点(分别记录为位点A、位点B和位点E),3个位点在朗德鹅中均有多态性。E位点中FAS~(EE)基因型个体在活重、填饲期增重、屠体重、半净膛重、全净膛重、胸肌重、腿肌重等性状表现中均比FAS~(ee)基因型个体高出12.78%-19.67%之间(P＜0.05)。位点B具有类似的影响趋势,而位点A对屠宰性能性状表现影响不显著。在3个酶切位点中,位点A和位点B的杂合子基因型FAS~(Aa)和FAS~(Bb)个体,产肝性能比相应纯合子个体好,而E位点的未突变个体FAS~(EE)基因型个体肝重比突变个体高33.09%(P＜0.05)。3个酶切位点在体脂沉积性状中差异不显著。
120 of healthy Landes Geese,14 weeks old,with similar body weight,were randomly divided into 6 groups and 20 each group according to the factor experiment design.Corn was the major force-feeding feedstuff.Diets were supplied with fatty acid:plus 6%of sunflower seed oil in the sunflower seed oil group,6%of rapeseed oil in the rapeseed oil group,6%of beef tallow in the beef tallow group,3%of goose oil in the first goose oil group,and 2%of goose oil in the second goose oil group in the base of the daily feedstuff. The others were consistent with the experimental group except the control group not adding any addition to fatty acids.The results as follows:weight of fatty liver in the sunflower seed oil group was more than other groups,and the liver weight of that group was as 1.37 times as that of control group,with significant difference(P＜0.05),while decreased in the beef tallow group.Effect of fatty acid on slaughter performance was less than on fatty liver weight,and slaughter traits in group added with rapeseed oil was higher than other groups,with significant difference in living weight,increased weight in force-feeding period,carcass weight and semi-eviscerated weight compared with the control group(P＜0.05).Weight of abdominal fat deposition in the group was also the highest,with significant difference compared with control group(P＜0.05),but not significant among the trial groups.
PCR-RFLP was designed in this study to investigatet the polymorphism in AfaⅠof FAS gene promoter region of landes geese.There are three AfaⅠrestriction sites in FAS gene promoter region(site A,site B and site E respectively in the fragment),which were polymorphism in landes geese.Genotype FAS~(EE) were higher than FASee 12.78%-19.67% (P＜0.05) at site E in living weight,feeding increase stage,carcass weight,halfeviscerate weight,eviscerate weight,breast muscle weight,leg muscle weight,the same to site B but less dependability to site A.To fatty liver performance,heterozygote was better than homozygote in site A and site B,the weight of liver of genotype FAS~(EE) is 33.09%(P＜0.05) higher than heterogenesis of site E.It is less dependability in lipidosis with the three restriction sites.
本研究还采用PCR-RFLP技术,分析了FAS基因启动子区在朗德鹅中的AfaⅠ酶切片段多态性分布。FAS基因启动子区具有3个AfaⅠ酶切位点(分别记录为位点A、位点B和位点E),3个位点在朗德鹅中均有多态性。E位点中FAS~(EE)基因型个体在活重、填饲期增重、屠体重、半净膛重、全净膛重、胸肌重、腿肌重等性状表现中均比FAS~(ee)基因型个体高出12.78%-19.67%之间(P＜0.05)。位点B具有类似的影响趋势,而位点A对屠宰性能性状表现影响不显著。在3个酶切位点中,位点A和位点B的杂合子基因型FAS~(Aa)和FAS~(Bb)个体,产肝性能比相应纯合子个体好,而E位点的未突变个体FAS~(EE)基因型个体肝重比突变个体高33.09%(P＜0.05)。3个酶切位点在体脂沉积性状中差异不显著。
120 of healthy Landes Geese,14 weeks old,with similar body weight,were randomly divided into 6 groups and 20 each group according to the factor experiment design.Corn was the major force-feeding feedstuff.Diets were supplied with fatty acid:plus 6%of sunflower seed oil in the sunflower seed oil group,6%of rapeseed oil in the rapeseed oil group,6%of beef tallow in the beef tallow group,3%of goose oil in the first goose oil group,and 2%of goose oil in the second goose oil group in the base of the daily feedstuff. The others were consistent with the experimental group except the control group not adding any addition to fatty acids.The results as follows:weight of fatty liver in the sunflower seed oil group was more than other groups,and the liver weight of that group was as 1.37 times as that of control group,with significant difference(P＜0.05),while decreased in the beef tallow group.Effect of fatty acid on slaughter performance was less than on fatty liver weight,and slaughter traits in group added with rapeseed oil was higher than other groups,with significant difference in living weight,increased weight in force-feeding period,carcass weight and semi-eviscerated weight compared with the control group(P＜0.05).Weight of abdominal fat deposition in the group was also the highest,with significant difference compared with control group(P＜0.05),but not significant among the trial groups.
PCR-RFLP was designed in this study to investigatet the polymorphism in AfaⅠof FAS gene promoter region of landes geese.There are three AfaⅠrestriction sites in FAS gene promoter region(site A,site B and site E respectively in the fragment),which were polymorphism in landes geese.Genotype FAS~(EE) were higher than FASee 12.78%-19.67% (P＜0.05) at site E in living weight,feeding increase stage,carcass weight,halfeviscerate weight,eviscerate weight,breast muscle weight,leg muscle weight,the same to site B but less dependability to site A.To fatty liver performance,heterozygote was better than homozygote in site A and site B,the weight of liver of genotype FAS~(EE) is 33.09%(P＜0.05) higher than heterogenesis of site E.It is less dependability in lipidosis with the three restriction sites.
引文
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