水稻（Oryza sativa L.）直立卷叶突变体比较蛋白质组学研究
摘要
水稻(Oryza sativa L.)是世界上重要的粮食作物,而叶片是水稻进行光合作用和呼吸作用的主要器官。直立卷叶的水稻由于其在种植时通风良好,光合作用充分,还可以减少病虫害的发生,但目前并没有直立叶片的水稻品种得到大面积种植。为了研究该突变体发生突变的分子机理,我们利用高通量的蛋白质组学技术平台分析了野生型水稻T65和直立卷叶突变体1317幼苗期叶,分蘖期叶枕、叶脉和叶片。对于双向电泳得到的差异蛋白点进行CapLC-ESI-Q-TOF MS/MS鉴定,得到了16个差异蛋白质点。其中,幼苗期水稻经分析比对无差异蛋白点,可能是由于该阶段卷叶表型尚未出现,还没有差异表达蛋白质。通过生物信息学分析及保守功能域的分析表明,16个差异点代表13个不同的蛋白质。这些差异蛋白质中有一些是参与糖和能量代谢过程,如葡糖磷酸变位酶和磷酸葡糖异构酶等；另外一些主要参与蛋白质的合成和加工、胁迫响应等过程,如HSP70、蛋白二硫键异构酶。在T65和1317中鉴定出的这些差异蛋白质点,为更好地理解植物中叶片直立的机制提供了新的分子基础。
Rice (Oryza sativa L.) is an important stable food crop in the world and its leaf is the main organ that carries out photosynthesis and respiration. Rice with erect leaf facilitates sufficient ventilation, efficient photosynthesis and to reduce the incidence of pests and diseases. Therefore, it is theoretical and practical guidance to research the molecular mechanism on the erect leaf mutant in rice. Using high-throughput proteomics technology, the different proteins between of the T65 and 1317 were analyzed from their seedling leaves, tiller leaves, leaf cushions and leaf vein, respectively. Using CapLC-ESI-Q-TOF MS/MS mass spectrometry, a total of 16 proteins were identified. These proteins were categorized into classes related to energy and proteins metabolism such as phosphoglucomutase and phosphoglucose isomerase; regulatory proteins such as HSP70, protein disulfide isomerase, which are mainly involved in protein synthesis and processing, stress response and other processes. Thees differentially expressed proteins between the T65 and 1317 provides new insights for further understanding the molecular mechanisms of erect leaf in plants.
Rice (Oryza sativa L.) is an important stable food crop in the world and its leaf is the main organ that carries out photosynthesis and respiration. Rice with erect leaf facilitates sufficient ventilation, efficient photosynthesis and to reduce the incidence of pests and diseases. Therefore, it is theoretical and practical guidance to research the molecular mechanism on the erect leaf mutant in rice. Using high-throughput proteomics technology, the different proteins between of the T65 and 1317 were analyzed from their seedling leaves, tiller leaves, leaf cushions and leaf vein, respectively. Using CapLC-ESI-Q-TOF MS/MS mass spectrometry, a total of 16 proteins were identified. These proteins were categorized into classes related to energy and proteins metabolism such as phosphoglucomutase and phosphoglucose isomerase; regulatory proteins such as HSP70, protein disulfide isomerase, which are mainly involved in protein synthesis and processing, stress response and other processes. Thees differentially expressed proteins between the T65 and 1317 provides new insights for further understanding the molecular mechanisms of erect leaf in plants.
引文
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[44]Morinaka Y, Sakamoto T, Inukai Y, et al. Morphological alteration caused by brassinosteroid insensitivity increases the biomass and grain production of rice. Plant Physiol,2006,141: 924～931
[45]Sinclair TR, Sheehy JE. Erect leaves and photosynthesis in rice. Science,2006,283:1456～1457
[46]Sakamoto T, Morinaka Y, Ohnishi T, Sunohara H, et al. Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nature Biotechn,2006,24: 105～109
[47]Yuan LP. Hybrid rice breeding for super yield. Hybrid Rice,1997,12:1～7
[48]Yamamuro C, Ihara Y, Wu X, et al. Loss of function of a rice brassinosteroid insensitivel homolog prevents internode elongation and bending of the lamina joint. Plant Cell,2000,12: 1591-1605
[49]Hong Z, Tanaka UM, Umemura K, et al. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell,2003,15: 2900～2910
[50]Mitchell JW, Mandava NB, Worley JF, et al. Brassins:A new family of plant hormones from rape pollen. Nature,1970,225:1065～1066
[51]Wang Z Y, Wang Q, Chong K, et al. The brassinosteroid signal transduction pathway. Cell Res,2006,16:427～434
[52]Jeong DH, Lee S, Kim SL, et al. Regulation of brassinosteroid responses by phytochrome B in rice. Plant Cell Environ,2007,30:590～599
[53]储昭庆,李李,宋丽,薛红卫.油菜素内酯生物合成与功能的研究进展.植物学通报,2006,23：543-555
[54]Kinoshita T, Delgado A, Seto H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature,2005,433:167～171
[55]Bai MY, Zhang LY, Gampala SS, et al. Functions of OsBZRl and 14-3-3 proteins in brassinosteroid signaling in rice. PNAS,2007,104:13839～13844
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