荸荠多糖中蛋白含量的测定
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摘要
荸荠是一种药食同源植物,为了建立荸荠多糖中蛋白含量的测定方法,采用水提醇沉法提取荸荠多糖,考马斯亮蓝法测定蛋白含量,比较了木瓜蛋白酶-Sevage法、Sevage法、三氯乙酸—正丁醇法脱蛋白的差异。结果表明,木瓜蛋白酶—Sevage法、Sevage法、三氯乙酸—正丁醇法的多糖损失率分别为21.92%、31.88%、47.05%,蛋白去除率分别为68.75%、47.08%、70.23%。考马斯亮蓝法适合荸荠多糖含量的测定,木瓜蛋白酶—Sevage法对于荸荠多糖是一种可行的脱蛋白方法。
To determine the protein content in polysaccharides from Eleocharis dulcis with multifunctions of both medicine and foods. The water extraction and alcohol precipitation method were adopted to obtain polysaccharide, Coomassie brilliant blue method was used to detect the protein content. The Sevage method, combined methods of papain and Sevage, combined methods of trichloroacetic acid and n-butanol were adopted to remove the protein respectively, and the effects of different methods were compared. The percentage of the removed protein for combined methods of papain and Sevage, Sevage method, combined methods of trichloroacetic acid and n-butanol were 68.75%, 47.08%and 70.23% respectively, and the loss rate of polysaccharides were 21.92%, 31.88% and 47.05% differently. Coomassie brilliant blue method was suitable for determination of protein in polysac-charides from Sevage. The combined methods of papain and Sevage was the best for removing protein for Eleocharis dulcis.
To determine the protein content in polysaccharides from Eleocharis dulcis with multifunctions of both medicine and foods. The water extraction and alcohol precipitation method were adopted to obtain polysaccharide, Coomassie brilliant blue method was used to detect the protein content. The Sevage method, combined methods of papain and Sevage, combined methods of trichloroacetic acid and n-butanol were adopted to remove the protein respectively, and the effects of different methods were compared. The percentage of the removed protein for combined methods of papain and Sevage, Sevage method, combined methods of trichloroacetic acid and n-butanol were 68.75%, 47.08%and 70.23% respectively, and the loss rate of polysaccharides were 21.92%, 31.88% and 47.05% differently. Coomassie brilliant blue method was suitable for determination of protein in polysac-charides from Sevage. The combined methods of papain and Sevage was the best for removing protein for Eleocharis dulcis.
引文
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[11] 罗群.考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量[J].成都大学学报(自然科学版),2014,33(2):125-126+129.
[12] 蔡亮亮,李西海.梅花入骨丹多糖中蛋白含量的测定[J].福建中医药大学学报,2012,22(3):37-39.
[13] 张勇,周丽明,黄章平.比清除率衡量Sevage法脱茶籽多糖蛋白的效果[J].南方农业学报,2016,47(1):107-111.
[2] 邱硕.苦荞多糖的提取及抗氧化性探析[J].微量元素与健康研究,2015,32(6):39-40.
[3] 严芬娟.荸荠高产栽培技术[J].现代农业科技,2015(24):105+111.
[4] 李长乐,潘永贵.响应面法优化超声波辅助提取鲜切荸荠黄化组织中黄酮类物质工艺研究[J].保鲜与加工,2017,17(4):30-36.
[5] 沈育伊,张德楠,王新桂,等.会仙喀斯特湿地野生荸荠微量元素及营养成分分析[J].微量元素与健康研究,2018,35(1):42-45.
[6] 伍淑婕,黄双全,聂辉,等.荸荠皮活性成分提取分离研究进展[J].食品科技,2018,43(10):289-293.
[7] 周耀明,姜英杰.正交试验优化荸荠皮色素提取工艺的研究[J].农产品加工(学刊),2014(14):35-37.
[8] Mourao P A S,Bastos I G.Highly acidic glycans from seacucumbers:isolation and fractionation of fucose-rich sulfated polysaccharides from the body wall of Ludwigothurea grisea[J].Eur J Biochem,1987,166(3):639-645.
[9] Albano RM,Mourao PAS.Isolation,fractionation,and preliminary characterization of a novel class of sulfated glycans from the tunic of Styela plicata (Chordata tunicata)[J].Biol Chem,1986,261(2):758-765.
[10] 张睢杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,200l.128-185.
[11] 罗群.考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量[J].成都大学学报(自然科学版),2014,33(2):125-126+129.
[12] 蔡亮亮,李西海.梅花入骨丹多糖中蛋白含量的测定[J].福建中医药大学学报,2012,22(3):37-39.
[13] 张勇,周丽明,黄章平.比清除率衡量Sevage法脱茶籽多糖蛋白的效果[J].南方农业学报,2016,47(1):107-111.