抑制金黄色葡萄球菌、大肠杆菌和黄曲霉菌的乳酸菌作用对比
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摘要
本研究分离筛选乳酸菌并进行抑菌试验比较研究,采用双层平板法从发面面肥、核桃叶青贮、玉米青贮、棉秸秆黄贮中共分离出31株乳酸菌。通过抑菌实验筛选出发面面肥中的F3和F11,核桃叶青贮中的HTY1. 9,玉米青贮中的YM1. 1和棉秸秆黄贮中的MG1. 5,共5株乳酸菌具有抑菌作用;抑制金黄色葡萄球菌效果最好为HTY1. 9,抑菌直径为15±0. 15 mm,抑制大肠杆效果最明显的是YM1. 1,抑菌直径是11. 5±0. 17 mm;对黄曲霉菌抑菌效果最好的是F11,抑菌直径为25±0. 12mm。经16S r DNA序列分析鉴定,F3与芽孢杆菌的相似性为100%,F11与屎肠球菌的相似性为100%,HTY1. 9与植物乳杆菌的相似性为99. 68%,YM1. 1与布氏杆菌的相似性为99. 52%,MG1. 5与魏斯氏乳酸菌的相似性为99. 32%;具有抑菌作用的5株乳酸菌分别为芽孢杆菌(Bacillus altitudinis)、屎肠球菌(Enterococcus faecium)、植物乳杆菌(Lactobacillus plantarum)、布氏杆菌(Brucella)和魏斯氏乳酸菌(Lactobacillus Weiss)。
In this study,lactic acid bacteria was isolated from Xinjiang traditional sourdough samples,walnut leaf ensiling,corn silage and cotton straw ammonification,and their bacteriostatic tests were carried out. The experiment was used the double deck plate method were isolated from sourdough samples,walnut leaf ensiling,corn silage,cotton straw ammonification,and five of them could inhibit indicative bacteria. The bacteriostatic effect was evaluated by the size of bacteriostasis. The best bacteriostasis of Staphylococcus aureus was HTY1. 9,the diameter of bacteriostasis was 15 ± 0. 15 mm,the most obvious effect of inhibiting the coliform was YM1. 1,the bacteriostasis diameter was 11. 5 ± 0. 17 mm,and The best bacteriostasis effect for Aspergillus flavus was F11 and the diameter of bacteriostasis was 25 ± 0. 12 mm. Based on 16 S r DNA gene sequnce analysis the similarity between strain F3 and Bacillus altitudinis was 100%,The similarity between strain F11 and Enterococcus faecium was 100%,HTY1. 9 and Lactobacillus plantarum had the similarity of 99. 68%,YM1. 1 and Brucella had the similarity of 99. 52%,the similarity between strain MG1. 5 and Weiss lactobacillus was 99. 32%. Five lactic acid bacteria were identified as Bacillus altitudinis,Enterococcus faecium,Lactobacillus plantarum,Brucella and Lactobacillus Weiss.
In this study,lactic acid bacteria was isolated from Xinjiang traditional sourdough samples,walnut leaf ensiling,corn silage and cotton straw ammonification,and their bacteriostatic tests were carried out. The experiment was used the double deck plate method were isolated from sourdough samples,walnut leaf ensiling,corn silage,cotton straw ammonification,and five of them could inhibit indicative bacteria. The bacteriostatic effect was evaluated by the size of bacteriostasis. The best bacteriostasis of Staphylococcus aureus was HTY1. 9,the diameter of bacteriostasis was 15 ± 0. 15 mm,the most obvious effect of inhibiting the coliform was YM1. 1,the bacteriostasis diameter was 11. 5 ± 0. 17 mm,and The best bacteriostasis effect for Aspergillus flavus was F11 and the diameter of bacteriostasis was 25 ± 0. 12 mm. Based on 16 S r DNA gene sequnce analysis the similarity between strain F3 and Bacillus altitudinis was 100%,The similarity between strain F11 and Enterococcus faecium was 100%,HTY1. 9 and Lactobacillus plantarum had the similarity of 99. 68%,YM1. 1 and Brucella had the similarity of 99. 52%,the similarity between strain MG1. 5 and Weiss lactobacillus was 99. 32%. Five lactic acid bacteria were identified as Bacillus altitudinis,Enterococcus faecium,Lactobacillus plantarum,Brucella and Lactobacillus Weiss.
引文
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[14]李院,魏新元,王静,等.抑制青霉菌乳酸菌的分离、鉴定及抑菌物质分析[J].食品科学,2015,36(21):150-155.
[15]刘红美,胡越,李玉权,等.短短芽孢杆菌DFG对金黄色葡萄球菌的抑制效果[J].贵州农业科学,2016,44(11):98-103.
[16]仝永娟,萨仁娜,张宏福,等.三株芽孢杆菌抑菌活性分析及对肉鸡舍空气微生物的影响[J].中国农业科学,2013,46(20):4344-4353.
[17]胡雪萍,王萍.芽孢杆菌抗菌作用研究[J].江西农业大学学报,2007(6):890-893.
[18]Bybee SN,Scorza AV,Lappin MR. et al. Effect of the pro-biotic Enterococcus faecium SF68 on presence of diarrheain cats and dogs housed in an animal shelter[j]. Vet InternMed. 2011 Jul-Aug; 25(4):856-60.
[19]Hamidi A,Mirnejad R,Yahaghi E,et al. The aflatoxin B1isolating potential of two lactic acid bacteria. Asian Pac JTrop Biomed. 2013 Sep; 3(9):732-6.
[20]Bluma RV,Etcheverry MG. Influence of Bacillus spp. iso-lated from maize agroecosystem on growth and aflatoxin B(1)production by Aspergillus section Flavi[j]. Pest ManagSci. 2006 Mar; 62(3):242-51.
[21]Moyne AL,Shelby R,Cleveland TE,et al. BacillomycinD:an iturin with antifungal activity against Aspergillus fla-vus[j]. Appl Microbiol. 2001 Apr; 90(4):622-9.
[22]董菁,薛正莲,梁慧.具抑菌活性的乳酸菌的筛选[J].安徽工程大学学报,2013,28(04):19-22.
[23]Taylor CC,Kung L Jr. The Effect of Lactobacillus buchneri40788 on the Fermentation and Aerobic Stability of HighMoisture Corn in Laboratory Silos[J]. J Dairy Sci,2002,85(6):1526-32.
[24]Patterson MF,McKay AM,Connolly M,et al. Effect ofhigh pressure on the microbiological quality of cookedchicken during storage at normal and abuse refrigerationtemperatures[J]. Food Microbiol. 2010,27(2):266-73.
[25]王海娟,戴雨珂,潘渠.魏斯氏菌的研究现状[J].成都医学院学报,2014,9(6):747-750.
[2]李丽民,吴先华,徐礼锋,等.金黄色葡萄球菌的临床分布及耐药性分析[J].中华医院感染学杂志,2014,24(4):787-789.
[3]向红,周藜,廖春,等.金黄色葡萄球菌及其引起的食物中毒的研究进展[J].中国食品卫生杂志,2015,27(2):196-199.
[4]杨生瑞,屈凌波,孙长坡,等.黄曲霉菌株的分离、鉴定及产毒能力分析[J].中国粮油学报,2012,27(6):110-114+123.
[5]崔晓霞,王秀敏,侯晓礁,等. 13种抗生素类药物对大肠杆菌的体外抑菌试验[J].吉林畜牧兽医,2008(7):9-10.
[6]Rao KP,Chennappa G,Suraj U,et al. Probiotic potentialof lactobacillus strains isolated from sorghum-based tradi-tional fermented food. Probiotics Antimicrob Proteins. 2015Jun; 7(2):146-56.
[7]Aoudia N,Rieu A,Briandet R,et al. Biofilms of Lactobacil-lus plantarum and Lactobacillus fermentum:Effect on stressresponses,antagonistic effects on pathogen growth and im-munomodulatory properties. Food Microbiol. 2016 Feb; 53(Pt A):51-9.
[8]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:364-370.
[9]钱洋.乳酸菌对食品中常见霉菌的抑制和黄曲霉素的去除[D].山东大学,2012.
[10]叶淑红,何连芳.植物乳杆菌的筛选及其在泡菜中的应用[J].中国酿造,2004(7):19-21.
[11]Scarpellini M,Mora D,Colombo S,et al. Development ofgenus/species-specific PCR analysis for identification ofCarnobacterium strains[j]. Curr Microbiol. 2002 Jul; 45(1):24-9.
[12]叶淑红,何连芳.植物乳杆菌的筛选及其在泡菜中的应用[J].中国酿造,2004(7):19-21.
[13]周配东,潘道东,张玉千,等.产细菌素乳酸菌的筛选及其所产细菌素的特性[J].食品科学,2011,32(17):303-307.
[14]李院,魏新元,王静,等.抑制青霉菌乳酸菌的分离、鉴定及抑菌物质分析[J].食品科学,2015,36(21):150-155.
[15]刘红美,胡越,李玉权,等.短短芽孢杆菌DFG对金黄色葡萄球菌的抑制效果[J].贵州农业科学,2016,44(11):98-103.
[16]仝永娟,萨仁娜,张宏福,等.三株芽孢杆菌抑菌活性分析及对肉鸡舍空气微生物的影响[J].中国农业科学,2013,46(20):4344-4353.
[17]胡雪萍,王萍.芽孢杆菌抗菌作用研究[J].江西农业大学学报,2007(6):890-893.
[18]Bybee SN,Scorza AV,Lappin MR. et al. Effect of the pro-biotic Enterococcus faecium SF68 on presence of diarrheain cats and dogs housed in an animal shelter[j]. Vet InternMed. 2011 Jul-Aug; 25(4):856-60.
[19]Hamidi A,Mirnejad R,Yahaghi E,et al. The aflatoxin B1isolating potential of two lactic acid bacteria. Asian Pac JTrop Biomed. 2013 Sep; 3(9):732-6.
[20]Bluma RV,Etcheverry MG. Influence of Bacillus spp. iso-lated from maize agroecosystem on growth and aflatoxin B(1)production by Aspergillus section Flavi[j]. Pest ManagSci. 2006 Mar; 62(3):242-51.
[21]Moyne AL,Shelby R,Cleveland TE,et al. BacillomycinD:an iturin with antifungal activity against Aspergillus fla-vus[j]. Appl Microbiol. 2001 Apr; 90(4):622-9.
[22]董菁,薛正莲,梁慧.具抑菌活性的乳酸菌的筛选[J].安徽工程大学学报,2013,28(04):19-22.
[23]Taylor CC,Kung L Jr. The Effect of Lactobacillus buchneri40788 on the Fermentation and Aerobic Stability of HighMoisture Corn in Laboratory Silos[J]. J Dairy Sci,2002,85(6):1526-32.
[24]Patterson MF,McKay AM,Connolly M,et al. Effect ofhigh pressure on the microbiological quality of cookedchicken during storage at normal and abuse refrigerationtemperatures[J]. Food Microbiol. 2010,27(2):266-73.
[25]王海娟,戴雨珂,潘渠.魏斯氏菌的研究现状[J].成都医学院学报,2014,9(6):747-750.