摘要
目的探讨采用高效液相色谱法(HPLC)同时测定通络驻景丸中的金丝桃苷和毛蕊花糖苷两种有效成分。方法使用Agilent ZORBAX SB-C18(4.6 mm×250 mm×5μm)作为色谱柱,以乙腈和0.1%的醋酸溶液作为流动相,梯度洗脱,流动相的流速为1 mL/min,检测波长分别为360 nm和334 nm。结果通络驻景丸中金丝桃苷和毛蕊花糖苷两种有效成分的峰面积与其进样量有较好的线性关系,并且它们的相关系数均超过0.999 3,所使用仪器精密度的标准差不超过2%,平均回收率均大于95%。结论采用高效液相色谱法测定通络驻景丸中金丝桃苷和毛蕊花糖苷这两种有效成分,快速、简便、回收率高,是测定通络驻景丸中这两种标志性有效成分的有效方法。
Objective To explore the use of high performance liquid chromatography(HPLC) to determine the two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills.Methods Agilent ZORBAX SBC18(4.6 mm×250 mm×5 μ m) was used as chromatographic column,acetonitrile and 0.1% acetic acid solution as mobile phase,gradient elution.The flow rate of mobile phase was 1 mL/min,detection wavelength was 360 nm and 334 nm respectively.Results The sample volume and peak area of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills has a good linear relationship.Their correlation coefficients exceeded 0.999 3.The standard deviation of the precision of the instrument used does not exceed 2% and the average recovery rate was greater than 95%.Conclusion The method of HPLC determination of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills is high-speed,easy,high recovery and this method is very effective.
引文
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