嘉陵江合川段秋季微生物多样性研究
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摘要
为了解嘉陵江合川段秋季微生物多样性,通过16S rRNA基因扩增和测序分析了2017年9月嘉陵江合川段利泽、渠江口、涪江口、草街4个监测点水样和底泥微生物的多样性和种类结构。结果显示:河流上下游水体中微生物物种组成相似、有延续性,利泽和涪江口,渠江口和草街水体中微生物组成均呈现相似;底泥中微生物组成与小区域水文环境相关,涪江口和渠江口水文环境相似,底泥中微生物群落结构也呈现相似;缓流环境更适应微生物生存繁殖,处于监测尾端的草街水体和底泥中微生物数量最多;环境影响因子中总磷、总氮和pH对水体中微生物群落结构组成表现出较大影响;水体中变形菌门、放线菌门、拟杆菌门和蓝细菌门较为丰富,显示该水域有富营养化的趋势。
Water sample,sediment microbial diversity and species structure from four monitoring points of Lize,Qujiangkou,Fujiangkou,Caojie in Hechuan reach of Jialing River were analyzed by 16 S rRNA gene amplification and sequencing in September 2017 to study the microbial diversity in autumn of these regions.The results showed that the composition of microbial species in water from upstream to downstream were similar and constant,The microbial compositions of water in Lize and Fuljiangkou were similar with that in Qujiangkou and CaojieThe microbial compositions in sediment were related to the hydrological environment in small areas.The hydrological environment of Fujiangkou was similar with that of Qujiangkou.Meanwhile,the microbial compositions in sediment were similar from these two monitoring points,either.The number of microbes was the highest in the water and sediment of Caojie because the slow-flow environment was more suitable for living and breeding of microorganisms.The microbial community structure in water was influenced greatly by TP,TN and pH.In addition,Proteobacteria,Actinobacteria, Bacteroidetes and Cyanobacteria were abundant in water,which indicated the trend of eutrophication.
Water sample,sediment microbial diversity and species structure from four monitoring points of Lize,Qujiangkou,Fujiangkou,Caojie in Hechuan reach of Jialing River were analyzed by 16 S rRNA gene amplification and sequencing in September 2017 to study the microbial diversity in autumn of these regions.The results showed that the composition of microbial species in water from upstream to downstream were similar and constant,The microbial compositions of water in Lize and Fuljiangkou were similar with that in Qujiangkou and CaojieThe microbial compositions in sediment were related to the hydrological environment in small areas.The hydrological environment of Fujiangkou was similar with that of Qujiangkou.Meanwhile,the microbial compositions in sediment were similar from these two monitoring points,either.The number of microbes was the highest in the water and sediment of Caojie because the slow-flow environment was more suitable for living and breeding of microorganisms.The microbial community structure in water was influenced greatly by TP,TN and pH.In addition,Proteobacteria,Actinobacteria, Bacteroidetes and Cyanobacteria were abundant in water,which indicated the trend of eutrophication.
引文
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[3]李正华.东平湖浮游细菌群落多样性分析[D].山东泰安:山东农业大学,2010.
[4]刘驰,李家宝,芮俊鹏,等.16S rRNA基因在微生物生态学中的应用[J].生态学报,2015,35(9):2769-2788.
[5]Caporaso J G,Lauber C L,Walters W A,et al.Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].PNAS,2011,108(1):4516-4522.
[6]孙巍.东江微生物的群落结构及其在氨氮转化中的作用特点[D].广州:华南理工大学,2014.
[7]叶文瑾.太湖富营养化水体和底泥中微生物群落的分子生态学研究[D].上海:上海交通大学,2009.
[8]任丽平.嘉陵江(四川段)梯级开发的多尺度健康评价研究[D].重庆:重庆大学,2012.
[9]杨敏,张晟,刘朔孺.草街水库蓄水后嘉陵江浮游植物群落特征及水质评价[J].环境科学,2015,36(07):2480-2486.
[10]王艳.嘉陵江水体中有毒重金属(汞、砷)的研究[D].重庆:西南大学,2008.
[11]陈静.重庆嘉陵江流域水中有毒重金属铅、铬的研究[D].重庆:西南大学,2008.
[12]刘婷婷.嘉陵江水体中碳、氮、磷季节变化及其输出[D].重庆:西南大学,2009.
[13]蒙国湖.嘉陵江重庆主城段富营养化数值模拟研究[D].重庆:重庆大学,2009.
[14]王敏.嘉陵江出口段小环藻水华发生规律及其生物毒性研究[D].重庆:重庆大学,2009.
[15]奚万艳,吴鑫,叶文瑾,等.太湖梅溪湾水域蓝藻水华前与水华末期细菌群落结构的变化[J].应用与环境生物学报,2007,13(1):97-103.
[16]张旭.淡水水体底泥微生物群落特征及微生物指标研究评价[D].上海:上海大学,2016.
[17]Rashidan K K,Bird D F.Role of predatoty bacteria in the termination of a cyanobacterial bloom[J].Microb Ecol,2003,41:97-105.
[18]谭旭,茅台地区赤水河水体微生物多样性分析[D].北京:北京化工大学,2014.
[19]Gao Y,Wang C C,Zhang W G,et al.Vertical and horizontal assemblage patterns of bacterial communities in a eutrophic river receiving domestic wastewater in southeast China[J].Environ Pollut,2017,(270):469-478.
[20]宋兆齐,王莉,刘秀花,等.云南和西藏四处热泉中的厚壁菌门多样性[J].生物技术,2015,25(5):481-486.
[21]Eiler A,Bertilsson A.Composition of freshwater bacterial communities associated with cyanobacterial bloom in four Swedish lakes [J].Environ Microbiol,2004,6(12):1228-1243.
[22]袁兴程.平原河网区河流水质改善关键技术与水环境治理方案研究[D].南京:南京大学,2012.
[23]官雪芳,马丽娜,林斌,等.不同培养条件对阴沟肠杆菌乐果降解效能的影响[J].福建农业学报,2009(4):355-359.
[24]Wang J J,Shen J,Wu Y C,et al.Phylogenetic beta diversity in bacterial assemblagesacross ecosystems:deterministic versus stochastic processes[J].ISME Journal,2013,7:1310-1321.
[25]Sterner R W,Elser J J.Ecological Stoichiometry:the Biology of Elements from Molecules to the Biosphere[M].Princeton:Princeton University Press,2002.
[26]Glibert P M.Ecological stoichiometry and its implications for aquatic ecosystem sustainability[J].Curr Opin Env Sust,2012,4(3):272-277.
[27]黄艺,舒中亚.基于浮游细菌生物完整性指数的河流生态系统健康评价——以滇池流域为例[J].环境科学,2013,34(8):3010-3018.