慢性镉暴露对背角无齿蚌肝脏的氧化损伤效应
|
摘要
目的:探明氯化镉(CdCl2)暴露对背角无齿蚌肝脏中抗氧化酶活力及脂质过氧化的影响。方法:根据背角无齿蚌96 h镉离子(Cd~(2+))半致死剂量设置1个对照组和2个处理组(0.1和0.5 mg/L),分别检测镉暴露4周及镉清除4周期间背角无齿蚌肝脏中抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)]的活力和脂质过氧化物丙二醛(MDA)的含量。结果:不同浓度Cd~(2+)暴露对背角无齿蚌抗氧化酶活力及脂质过氧化产生不同程度的影响,低浓度Cd~(2+)暴露的毒性效应较弱,高浓度Cd~(2+)暴露可显著抑制肝脏SOD活力,诱导GPx活力升高,在暴露后期显著抑制CAT活力,MDA含量随着暴露时间的延长而显著升高。结论:慢性Cd~(2+)暴露可影响背角无齿蚌肝脏抗氧化酶活力,引起脂质过氧化损伤,且作用机制与急性毒性不同。
Objective:To explore the changes of antioxidant enzyme activities and lipid peroxidation in the liver of the freshwater museel Anodonta woodiana after chronic exposure to cadmium chloride(CdCl2).Methods:The freshwater mussels were exposed to different concentrations of Cd~(2+)(0, 0.1 and 0.5 mg/L) for 4 weeks, then they were removed to clean water and cultured for another 4 weeks. Activities of antioxidant enzymes[superoxide dismutase(SOD), glutathione peroxidase(GPx) and catalase(CAT)] and content of malondialdehyde(MDA) in the liver of the freshwater mussels were measured.Results:Different concentrations of Cd~(2+) exposure induced various changes of antioxidant enzyme activities and caused lipid peroxidation in the liver of the freshwater mussels. Toxic effect of low-dose Cd~(2+)was lower. While, high-dose Cd~(2+)exposure could induce severe oxidative stress such as inhibition of SOD activity, induction of GPx activity and increase in MDA content. Moreover, the persistent toxic effects were also found in the cleaning period.Conclusion:Chronic exposure of Cd~(2+)affected the activities of antioxidant enzymes and caused lipid peroxidation damage in the liver of the freshwater mussels. The chronic toxicity mechanism of pollutants was different from the acute toxicity which should not be ignored.
Objective:To explore the changes of antioxidant enzyme activities and lipid peroxidation in the liver of the freshwater museel Anodonta woodiana after chronic exposure to cadmium chloride(CdCl2).Methods:The freshwater mussels were exposed to different concentrations of Cd~(2+)(0, 0.1 and 0.5 mg/L) for 4 weeks, then they were removed to clean water and cultured for another 4 weeks. Activities of antioxidant enzymes[superoxide dismutase(SOD), glutathione peroxidase(GPx) and catalase(CAT)] and content of malondialdehyde(MDA) in the liver of the freshwater mussels were measured.Results:Different concentrations of Cd~(2+) exposure induced various changes of antioxidant enzyme activities and caused lipid peroxidation in the liver of the freshwater mussels. Toxic effect of low-dose Cd~(2+)was lower. While, high-dose Cd~(2+)exposure could induce severe oxidative stress such as inhibition of SOD activity, induction of GPx activity and increase in MDA content. Moreover, the persistent toxic effects were also found in the cleaning period.Conclusion:Chronic exposure of Cd~(2+)affected the activities of antioxidant enzymes and caused lipid peroxidation damage in the liver of the freshwater mussels. The chronic toxicity mechanism of pollutants was different from the acute toxicity which should not be ignored.
引文
[1]张宜奎,宋秀凯,刘爱英,等. Cd2+胁迫对文蛤鳃组织SOD、CAT活力及MDA含量的影响[J].安全与环境学报, 2011,11(6):19-23.
[2] Michele J, Hein H, Preez D, et al. The freshwater river crab Potamonautes warreni as a bioaccumulative indicator of iron and manganese pollution in two aquatic systems[J]. Ecotoxicol Environ Safety, 1998,41:203-214.
[3]刘智禹,吴欧燕,吴成业,等.日本对虾对镉富集能力的研究[J].福建水产, 2011,5:20-23.
[4]陆慧贤,徐永健.缢蛏(Sinonovacula constricta)对海水中镉富集规律的研究[J].生态科学, 2013,32(4):434-438.
[5]蔡荣,郭赛男,郑家浪. Cd2+暴露对斑马鱼肝脏和卵巢抗氧化和免疫系统的影响及蓝LED光预暴露的保护作用[J].生态毒理学报, 2018,13(1):169-178.
[6]李慧,狄桂兰,裴超,等.镉胁迫下鲤血液免疫指标的变化[J].水生态学杂志, 2017,38(4):90-95.
[7]刘建博,潘登,江安娜,等.镉暴露对文蛤雄性生殖细胞的影响[J].环境科学学报, 2013,33(7):2036-2043.
[8]刘建博,夏利平,徐瑞,等.镉离子对文蛤肝胰腺超微结构的影响[J].动物学杂志, 2014,49(5):727-735.
[9]李艳东.水体镉对中华绒螯蟹(Eriocheir sinensis)亲体毒性作用研究[D].上海:华东师范大学, 2007.
[10] Wang L, Pan L, Liu Na, et al. Biomarkers and bioaccumulation of clam Ruditapes philippinarum in response to combined cadmium and benzo[a]pyrene exposure[J]. Food Chem Toxicol, 2011,49:3407-3417.
[11]杨惠珍,刘娜,李涌泉,等.镉对背角无齿蚌主要组织谷胱甘肽含量和相关酶活性的影响[J].农业环境科学学报, 2015,34(1):15-21.
[12]杨晓婧,李美丽,白建华.火焰原子吸收光谱法测定废水中的重金属离子[J].光谱实验室, 2010,27(1):247-249.
[13] Roesijadi G, Robinson W. Metal regulation in aquatic animals:mechanisms of uptake, accumulation and release[M]//Malins D C, Ostrander G K. Aquatic toxicology--molecular, biochemical and cellular perspectives.London:Lewis Publishers, 1994:387-420.
[14]袁牧,王昌留,王一斐,等.超氧化物歧化酶的研究进展[J].中国组织化学与细胞化学杂志, 2016,25(6):550-558.
[15]沈敦瑜,郭顺勤.生物无机化学简明教程[M].成都:成都科技大学出版社, 1993:95-99.
[16] Tagliaferro M, Gon?alves A M M, Bergman M, et al.Assessment of metal exposure(uranium and copper)by the response of a set of integrated biomarkers in a stream shredder[J]. Ecol Indicators, 2018,95:991-1000.
[17]黄永洪,花慧,沈国强,等.猪肝过氧化氢酶提取条件的研究[J].生物技术通讯, 2006,16(1):40-42.
[18]邢慧芳,李涌泉,杨慧珍,等.镉对背角无齿蚌外套膜和鳃抗氧化酶活力及脂质过氧化的影响[J].环境科学学报, 2013,33(3):856-860.
[19]蔡垚.重金属镉对背角无齿蚌肝脏和肌肉过氧化氢酶活性的影响[J].南京晓庄学院学报, 2012,3:84-86.
[20] Sehonova P, Zikova A, Blahova J, et al. mRNA expression of antioxidant and biotransformation enzymes in zebrafish(Danio rerio)embryos after exposure to the tricyclic antidepressant amitriptyline[J]. Chemosphere,2019,217:516-521.
[21]邓思平,赵云涛,朱春华,等.镉对尖紫蛤抗氧化酶活性及脂质过氧化的影响[J].水生生物学报, 2012,36(4):689-695.
[22] Anwer T, Sharma M, Pillai K K, et al. Protective effect of bezafibrate on streptozotocin-induced oxidative stress and toxicity in rats[J]. Toxicology, 2007,229:165-172.
[2] Michele J, Hein H, Preez D, et al. The freshwater river crab Potamonautes warreni as a bioaccumulative indicator of iron and manganese pollution in two aquatic systems[J]. Ecotoxicol Environ Safety, 1998,41:203-214.
[3]刘智禹,吴欧燕,吴成业,等.日本对虾对镉富集能力的研究[J].福建水产, 2011,5:20-23.
[4]陆慧贤,徐永健.缢蛏(Sinonovacula constricta)对海水中镉富集规律的研究[J].生态科学, 2013,32(4):434-438.
[5]蔡荣,郭赛男,郑家浪. Cd2+暴露对斑马鱼肝脏和卵巢抗氧化和免疫系统的影响及蓝LED光预暴露的保护作用[J].生态毒理学报, 2018,13(1):169-178.
[6]李慧,狄桂兰,裴超,等.镉胁迫下鲤血液免疫指标的变化[J].水生态学杂志, 2017,38(4):90-95.
[7]刘建博,潘登,江安娜,等.镉暴露对文蛤雄性生殖细胞的影响[J].环境科学学报, 2013,33(7):2036-2043.
[8]刘建博,夏利平,徐瑞,等.镉离子对文蛤肝胰腺超微结构的影响[J].动物学杂志, 2014,49(5):727-735.
[9]李艳东.水体镉对中华绒螯蟹(Eriocheir sinensis)亲体毒性作用研究[D].上海:华东师范大学, 2007.
[10] Wang L, Pan L, Liu Na, et al. Biomarkers and bioaccumulation of clam Ruditapes philippinarum in response to combined cadmium and benzo[a]pyrene exposure[J]. Food Chem Toxicol, 2011,49:3407-3417.
[11]杨惠珍,刘娜,李涌泉,等.镉对背角无齿蚌主要组织谷胱甘肽含量和相关酶活性的影响[J].农业环境科学学报, 2015,34(1):15-21.
[12]杨晓婧,李美丽,白建华.火焰原子吸收光谱法测定废水中的重金属离子[J].光谱实验室, 2010,27(1):247-249.
[13] Roesijadi G, Robinson W. Metal regulation in aquatic animals:mechanisms of uptake, accumulation and release[M]//Malins D C, Ostrander G K. Aquatic toxicology--molecular, biochemical and cellular perspectives.London:Lewis Publishers, 1994:387-420.
[14]袁牧,王昌留,王一斐,等.超氧化物歧化酶的研究进展[J].中国组织化学与细胞化学杂志, 2016,25(6):550-558.
[15]沈敦瑜,郭顺勤.生物无机化学简明教程[M].成都:成都科技大学出版社, 1993:95-99.
[16] Tagliaferro M, Gon?alves A M M, Bergman M, et al.Assessment of metal exposure(uranium and copper)by the response of a set of integrated biomarkers in a stream shredder[J]. Ecol Indicators, 2018,95:991-1000.
[17]黄永洪,花慧,沈国强,等.猪肝过氧化氢酶提取条件的研究[J].生物技术通讯, 2006,16(1):40-42.
[18]邢慧芳,李涌泉,杨慧珍,等.镉对背角无齿蚌外套膜和鳃抗氧化酶活力及脂质过氧化的影响[J].环境科学学报, 2013,33(3):856-860.
[19]蔡垚.重金属镉对背角无齿蚌肝脏和肌肉过氧化氢酶活性的影响[J].南京晓庄学院学报, 2012,3:84-86.
[20] Sehonova P, Zikova A, Blahova J, et al. mRNA expression of antioxidant and biotransformation enzymes in zebrafish(Danio rerio)embryos after exposure to the tricyclic antidepressant amitriptyline[J]. Chemosphere,2019,217:516-521.
[21]邓思平,赵云涛,朱春华,等.镉对尖紫蛤抗氧化酶活性及脂质过氧化的影响[J].水生生物学报, 2012,36(4):689-695.
[22] Anwer T, Sharma M, Pillai K K, et al. Protective effect of bezafibrate on streptozotocin-induced oxidative stress and toxicity in rats[J]. Toxicology, 2007,229:165-172.