摘要
试验旨在研究红景天提取物对高原缺氧条件下肉鸡接种法氏囊疫苗和绵羊红细胞后产生的特异性抗体水平的影响。随机选取100只1日龄健康AA肉仔鸡,随机分为5组。其中,对照组只饲喂基础日粮,其余4组分别在基础日粮中添加0.10%、0.15%、0.20%和0.40%红景天水提物干粉。每个组取半数鸡只于13日龄腹腔注射接种绵羊红细胞,另一半鸡只于20日龄口服接种法氏囊疫苗。研究发现,免疫接种法氏囊疫苗后2 d和19 d,相比于对照组,0.20%和0.40%红景天处理组显著提高了法氏囊疫苗抗体水平(P<0.05)。在腹腔注射绵羊红细胞后5 d、8 d和12 d时,相比对照组,0.20%和0.40%红景天处理组显著提高了绵羊红细胞抗体水平(P<0.05)。该试验表明红景天提取物可以提高高海拔缺氧条件下肉鸡特异性抗体水平,因此其可能作为高海拔缺氧条件下肉鸡日粮的一种免疫调节性添加剂。
In this study, we tested whether Rhodiola extracts had any effects on antibody responses to infectious bursal disease virus(IBDV) and sheep red blood cells(SRBC) in broiler chickens reared high-altitude hypoxia condition. A total of 1001-day-old healthy AA broilers were randomly divided into 5 treatments. Four treatments received dietary supplementation of 0.10%, 0.15%, 0.20% and 0.40% dry powder of Rhodiola aqueous extracts in basal diet, respectively, and the other group serving as control fed basal diet without supplementation. Half of the broilers in each treatment were injected intraperitoneally 1 mL of a 10% suspension of SRBC at 13 d of age, and the others were orally vaccinated at 20 d of age with the Bursine-2 live virus. The results showed that compared to the control group, supplementation of 0.20% and 0.40% Rhodiola extracts significantly increased the IBDV antibody titer at 2 d and 19 d after vaccination(P<0.05), and increased the SRBC antibody titer at 5 d, 8 d and 12 d after inoculation(P<0.05). In conclusion, dietary supplementation with Rhodiola extracts could increase the specific antibody level in broiler chickens reared at high-altitude hypoxia condition. Therefore, Rhodiola extracts may be act as an immune regulator additive for broilers reared high-altitude hypoxia condition.
引文
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