变性高效液相色谱技术在快速检测食品微生物中的应用研究
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摘要
食品安全问题一直是社会各界重点关注的热点话题。为了保证食品质量和安全,有必要对流入市场的食品进行食品中微生物含量的检验。变性高效液相色谱技术因其自身特点以及检验的精确度相对较高、灵敏度和速度相对较快等特点,在微生物检测方面得到了广泛应用。本文主要对变性高效液相色谱技术的实际应用进入深入研究,从原理、特点等方面进行详细阐述。
Food safety has always been a hot topic of social attention, in order to ensure the quality and safety of food to a certain extent. It is necessary to test the microbial content of food that has entered the market. Denaturing high performance liquid chromatography(denaturing high performance liquid chromatography) is characterized by its own characteristics and relatively high accuracy, sensitivity and speed of detection. It has been widely used in the detection of microorganisms. In this paper, denatured high performance liquid chromatography(HDLC) technology has been deeply studied in the practical application process. This paper elaborates its principle and characteristics in detail.
Food safety has always been a hot topic of social attention, in order to ensure the quality and safety of food to a certain extent. It is necessary to test the microbial content of food that has entered the market. Denaturing high performance liquid chromatography(denaturing high performance liquid chromatography) is characterized by its own characteristics and relatively high accuracy, sensitivity and speed of detection. It has been widely used in the detection of microorganisms. In this paper, denatured high performance liquid chromatography(HDLC) technology has been deeply studied in the practical application process. This paper elaborates its principle and characteristics in detail.
引文
[1]林黛琴,王婷婷,万承波.高效液相色谱-串联质谱法快速测定食品中5种罂粟壳生物碱[J].质谱学报,2017,3(2):239-247.
[2]马桂娟,汤丽华,朱捷.固相萃取-高效液相色谱法快速测定食品中7种合成着色剂[J].食品安全导刊,2017,3(4):53-54.
[3]江生,敖菊梅,潘正.高效液相色谱法同时快速测定饮料中的12种食品添加剂[J].分析试验室,2017,3(6):115-117.
[4]周霞,陈万勤,傅红雪.全自动固相萃取-超高效液相色谱法快速测定食品中米酵菌酸含量[J].安徽农业科学,2018,3(4):159-160.
[5]范维,高晓月,李贺楠.高通量微生物显色法快速检测动物源性食品中抗生素残留[J].食品科学,2017,5(16):245-250.
[6]方乐轩,冯嘉骏,莫浩斌.应用高效液相色谱法检测食品添加剂[J].食品安全导刊,2017,7(6):81-82.
[7]贾红岩,王亚涛,张芝华.高效液相色谱法测定不同产地及品种灵芝三萜类成分的含量[J].微生物学通报,2017,2(1):244-250.
[8]付珍珍,曾月,李增威.分子印迹固相萃取-高效液相色谱法测定牛奶中四环素类抗生素残留[J].浙江农业学报,2018,2(2):314-322.
[9]屠斌华,陈益,冯婧.柱前衍生化高效液相色谱法测定复方酮康唑乳膏中硫酸新霉素的含量[J].中国医药导刊,2018,9(6):356-359.
[2]马桂娟,汤丽华,朱捷.固相萃取-高效液相色谱法快速测定食品中7种合成着色剂[J].食品安全导刊,2017,3(4):53-54.
[3]江生,敖菊梅,潘正.高效液相色谱法同时快速测定饮料中的12种食品添加剂[J].分析试验室,2017,3(6):115-117.
[4]周霞,陈万勤,傅红雪.全自动固相萃取-超高效液相色谱法快速测定食品中米酵菌酸含量[J].安徽农业科学,2018,3(4):159-160.
[5]范维,高晓月,李贺楠.高通量微生物显色法快速检测动物源性食品中抗生素残留[J].食品科学,2017,5(16):245-250.
[6]方乐轩,冯嘉骏,莫浩斌.应用高效液相色谱法检测食品添加剂[J].食品安全导刊,2017,7(6):81-82.
[7]贾红岩,王亚涛,张芝华.高效液相色谱法测定不同产地及品种灵芝三萜类成分的含量[J].微生物学通报,2017,2(1):244-250.
[8]付珍珍,曾月,李增威.分子印迹固相萃取-高效液相色谱法测定牛奶中四环素类抗生素残留[J].浙江农业学报,2018,2(2):314-322.
[9]屠斌华,陈益,冯婧.柱前衍生化高效液相色谱法测定复方酮康唑乳膏中硫酸新霉素的含量[J].中国医药导刊,2018,9(6):356-359.