检测小鼠血清3DAg-SERS基底制备及表征
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摘要
腐蚀-原位生长方式制备3DAg-SERS活性针灸针基底并采用表面增强拉曼光谱法测定对氨基苯硫酚和小鼠血清样,结果表明:对氨基苯硫酚在10~(-12)～10~(-4)mol/L浓度的范围内线性关系良好,最低检出限为10-12mol/L;可用于小鼠血清样品的检测,且最低可检出10-5倍原血清稀释液;基底检测信号的重现性良好,RSD=2.74%。该SERS基底检测样品快速、准确且适合分析液体类样品,对微创临床取样、体液检测手段有一定的参考价值。
Corrosion-in situ growth method was used to prepare 3DAg-SERS active acupuncture needle base and surface-enhanced Raman spectroscopy was used to determine p-aminothiophenol and mouse serum samples.The results showed that the linear relationship is good in the range of 10~(-4)～10~(-12) mol/L,the lowest detection limit is 10~(-12)mol/L;it can be used for the detection of mouse serum samples with 10~(-5) times than before;The reproducibility of the detection signal is good,RSD=2.74%.The SERS base detection sample is fast,accurate and suitable for the analysis of liquid samples,and has certain reference value for minimally invasive clinical sampling and body fluid detection methods.
Corrosion-in situ growth method was used to prepare 3DAg-SERS active acupuncture needle base and surface-enhanced Raman spectroscopy was used to determine p-aminothiophenol and mouse serum samples.The results showed that the linear relationship is good in the range of 10~(-4)～10~(-12) mol/L,the lowest detection limit is 10~(-12)mol/L;it can be used for the detection of mouse serum samples with 10~(-5) times than before;The reproducibility of the detection signal is good,RSD=2.74%.The SERS base detection sample is fast,accurate and suitable for the analysis of liquid samples,and has certain reference value for minimally invasive clinical sampling and body fluid detection methods.
引文
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[3]Chen L,Gao Y,Cheng Y,et al.Nonresonant chemica mechanism in surface-enhanced raman scattering of pyridine on m@au 12 clusters[J].Nanoscale,2016,8(7):4086-4093.
[4]孙如,李淑瑾,姚建林,等.4-氨基苯硫酚在金电极上电化学转变过程的表面增强拉曼光谱及其理论研究[J].化学学报,2007,65(17):1741-1745.
[5]Li S X,Zeng Q Y,Li L F,et al.Study of support vector machine and serum surface-enhanced raman spectroscopy for noninvasive esophageal cancer detection[J].Journal o biomedical optics,2013,18(2):027008.
[6]Li P,Zhou B,Cao X,et al.Functionalized acupuncture needle as surface‐enhanced resonance raman spectroscopy sensor for rapid and sensitive detection of dopamine in serum and cerebrospinal fluid[J].Chemistry-A European Journal,2017,23(57):14278-14285.
[7]Unser S,Bruzas I,He J,et al.Localized surface plasmon resonance biosensing:Current challenges and approaches[J].Sensors,2015,15(7):15684-15716.
[8]Pattnaik P.Surface plasmon resonance[J].Appl Biochem Biotechnol,2005,126(2):79-92.
[9]Keller E L and Frontiera R R.Ultrafast nanoscale raman thermometry proves heating is not a primary mechanism for plasmon-driven photocatalysis[J].ACS nano,2018,12(6):5848-5855
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