摘要
为研究卵形鲳鲹(Trachinotus ovatus)幼鱼对饲料中维生素B_2的最适需要量,配制维生素B_2水平为10 mg·kg~(-1)、4.4 mg·kg~(-1)、6.2 mg·kg~(-1)、8.0 mg·kg~(-1)、11.3 mg·kg~(-1)、15.5 mg·kg~(-1)的6种实验饲料,每种饲料设3个平行,每个平行放25尾初始均质量为(11.63±0.17) g的卵形鲳鲹幼鱼,每日2次饱食,投喂8周。结果显示,4.4 mg·kg~(-1)组幼鱼的增重率、特定生长率均显著高于0 mg·kg~(-1)组(P<0.05),血清中谷草转氨酶、谷丙转氨酶活性显著低于0 mg·kg~(-1)组(P<0.05),但饲料中维生素B_2水平对饲料系数、成活率、肝脏维生素B_2含量、血清免疫指标、全鱼粗脂肪和粗蛋白、肌肉水分、粗脂肪和灰分均无显著影响(P>0.05);6.2 mg·kg~(-1)组肝脏D-氨基酸氧化酶(D-AAO)活性显著高于0 mg·kg~(-1)组(P<0.05)。折线模型分析显示,卵形鲳鲹获得最佳生长和肝脏D-AAO活力达到最大时对饲料中维生素B_2的需要量分别为4.23 mg·kg~(-1)和6.24 mg·kg~(-1)。
To determine the optimal dietary vitamin B_2 requirement of juvenile golden pompano(Trachinotus ovatus), six experimental diets were formulated which contained 0 mg·kg~(-1), 4.4 mg·kg~(-1), 6.2 mg·kg~(-1), 8.0 mg·kg~(-1), 11.3 mg·kg~(-1) and 15.5 mg·kg~(-1) of vitamin B_2. Each diet was assigned to three parallels of 25 fish [initial mass of(11.63±0.17) g] which were fed twice daily for eight weeks. The results show that the weight gain rate and specific growth rate of the fish fed with diet of 4.4 mg·kg~(-1) vitamin B_2 were significantly higher than those of 0 mg·kg~(-1) group(P<0.05), and the activities of aspartate aminotransferase and alanine aminotrans-ferase in the serum were significantly lower than those in 0 mg·kg~(-1) group(P<0.05). No significant effect was found on the fish coefficient, survival rate, liver vitamin B_2 content, serum immune parameters, crude protein and crude fat content in whole body, moisture, crude fat and crude ash in muscle(P>0.05); the liver D-AAO activity in 6.2 mg·kg~(-1) group was significantly higher in 0 mg·kg~(-1) group(P<0.05). Broken-line model analysis estimates that the dietary vitamin B_2 requirement of juvenile golden pompano is 4.23 mg·kg~(-1) for optimal growth, and 6.24 mg·kg~(-1) for maximum liver D-AAO activity.
引文
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