植物乳杆菌素BM-1对大肠杆菌抑菌活性的研究
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摘要
【目的】探究植物乳杆菌素BM-1对大肠杆菌的抑菌活性。【方法】通过管碟法检测植物乳杆菌素BM-1对12株大肠杆菌的抑菌活性,选取不同敏感型大肠杆菌5株,对大肠杆菌生长曲线、活菌数、生物膜的形成进行测定,并采用实时荧光定量PCR方法测定大肠杆菌中细菌素受体基因的表达。【结果】生长曲线、活菌数、生物膜的形成试验结果显示,大肠杆菌CGMCC 1.8723对植物乳杆菌素BM-1敏感性最强;实时荧光定量PCR显示植物乳杆菌素BM-1显著影响大肠杆菌甘露糖磷酸转移酶系统编码基因的表达。【结论】植物乳杆菌素BM-1对大肠杆菌表现出不同水平的抑菌活性。
【Objective】In this study the bacteriostatic activity ofplantaricin BM-1 against Escherichia coli was investigated.【Method】The bacteriostatic activity of plantaricin BM-1 on Escherichia coli was detected by Oxford Cup bacteriostatic method.Five Escherichia coli strains were selected from different sensitive to plantaricin BM-1,and their growth curves,viable counts,biofilm formation and real-time PCR were determined.【Result】Growth curve,viable count and biofilm formation showed that CGMCC 1.8723 was the most sensitive strain to plantaricin BM-1.Real-time PCR showed that plantaricin BM-1 stimulated the receptor gene expression.【Conclusion】Plantaricin BM-1 exhibited different levels of antibacterial activity against Escherichia coli.
【Objective】In this study the bacteriostatic activity ofplantaricin BM-1 against Escherichia coli was investigated.【Method】The bacteriostatic activity of plantaricin BM-1 on Escherichia coli was detected by Oxford Cup bacteriostatic method.Five Escherichia coli strains were selected from different sensitive to plantaricin BM-1,and their growth curves,viable counts,biofilm formation and real-time PCR were determined.【Result】Growth curve,viable count and biofilm formation showed that CGMCC 1.8723 was the most sensitive strain to plantaricin BM-1.Real-time PCR showed that plantaricin BM-1 stimulated the receptor gene expression.【Conclusion】Plantaricin BM-1 exhibited different levels of antibacterial activity against Escherichia coli.
引文
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[15]董雨馨,谢远红,张策,金君华,张红星.瑞士乳杆菌M14-1所产细菌素的分离纯化及抑菌特性分析[J].天然产物研究与开发,2018,30(8):1437-1443
[16]李世超,方华,朱校适.嗜酸乳杆菌产细菌素的生物学特性及抑菌机制研究[J].饲料研究,2017(11):20-26
[17]喻华英,陈绪松,贾桂珍,陈英.仔猪腹泻的大肠杆菌生物膜的筛选和致病力观察[J].中国兽医学报,2015,35(5):824-827
[18]吴思琪,宋静颐,秦倩,张策,金君华,谢远红,刘慧,张红星.一种高效稳定的微生物总RNA提取方法[J].江苏农业学报,2013,3(3):517-523
[19]唐婳,刘国生,谢志雄,沈萍.细菌生物膜的结构及形成机制研究进展[J].氨基酸和生物资源,2006,28(3):30-33
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[21]Cotter PD,Hill C,Ross RP.Bacteriocins:Developing innate immunity for food[J].Nature Reviews Microbiology,2005,3(10):777-788
[22]Stoll R,Goebel W.The major PEP-phosphotransferase systems(PTSs)for glucose mannose and cellobiose of Listeria monocytogenes,and their significance for extra-and intracellular growth[J].Microbiology,2010,156(4):1069-1083
[23]Kjos M,Nes IF,Diep DB.Mechanisms of resistance to bacteriocins targeting the mannose phosphotransferase system[J].Applied and Environmental Microbiology,2011,77(10):3335-3342
[2]王文婧,殷文政,冀昌龙.产抑菌活性物质菌株的筛选及其抑菌机理的研究[J].现代食品科技,2016(12):157-163
[3]Yu N,Chen ZJ.Screening of lactic acid bacteria for bacteriostatic activity and characteristics of anti-bacterial compounds[J].Food Science and Technology,2011,36(5):13-17
[4]周晔,王国霞,黄文庆,黄燕华.乳酸菌对南美白对虾保鲜效果的初步探讨[J].广东农业科学,2010,37(10):117-119
[5]Carr FJ,Chill D,Maida N.The lactic acid bacteria:a literature survey[J].Critical Reviews in Microbiology,2002,28:281-370
[6]张敏.乳酸菌素BM-1生物保鲜膜制备及在冷鲜肉保鲜中抑菌性研究[D].北京:北京农学院,2017:3-5
[7]沈莲清,苏光耀,王向阳.植物乳杆菌素研究进展[J].食品与生物技术学报,2006,25(5):121-126
[8]胡小媛,王安如,黄建忠,滕达,王建华.乳酸菌Ⅱa类细菌素抗菌机制的研究[J].中国饲料,2012(20):9-12
[9]Jack R W,Wan J,Gordon J,Harmark K,Davidson B,Hillier A J,Wettenhall R H,Hickey M W,Coventry M J.Characterization of the chemical and antimicrobial properties of piscicolin 126,a bacteriocin produced by Carnobacterium piscicola JG126[J].Applied&Environmental Microbiology,1996,62(8):2897-2903
[10]岳喜庆,闵钟熳,郭晨,蔡玮璠.产Ⅱa类细菌素乳酸菌的筛选及鉴定[J].江苏农业学报,2011,27(2):410-414
[11]马淑霞,李丽秋,丁思,任继秋,吴庆田,张昆,杨春佳,崔刚,胡新俊,代月,王春敏.片球菌素PediocinPA-1抑菌活性检测[J].中国微生态学杂志,2011,23(9):800-801
[12]Zhang HX,Liu L,Hao YL,Zhong SQ,Han T,Xie YH.I-solation and partial characterization of a bacteriocin produced byLactobacillus plantarum BM-1isolated from a traditionally fermented Chinese meat product[J].Microbiology and Immunology,2013,57(11):746-755
[13]董雨馨,张君超,张红星,谢远红.1株产细菌素植物乳杆菌的筛选及其细菌素抑菌性质研究[J].食品与发酵工业,2016,42(7):74-79
[14]杨雯舸,冯广莹,孙铭,金君华,张红星,谢远红.素复合抗菌膜的制备及其在冷鲜猪肉保鲜中的应用[J].北京农学院学报,2018,33(3):88-92
[15]董雨馨,谢远红,张策,金君华,张红星.瑞士乳杆菌M14-1所产细菌素的分离纯化及抑菌特性分析[J].天然产物研究与开发,2018,30(8):1437-1443
[16]李世超,方华,朱校适.嗜酸乳杆菌产细菌素的生物学特性及抑菌机制研究[J].饲料研究,2017(11):20-26
[17]喻华英,陈绪松,贾桂珍,陈英.仔猪腹泻的大肠杆菌生物膜的筛选和致病力观察[J].中国兽医学报,2015,35(5):824-827
[18]吴思琪,宋静颐,秦倩,张策,金君华,谢远红,刘慧,张红星.一种高效稳定的微生物总RNA提取方法[J].江苏农业学报,2013,3(3):517-523
[19]唐婳,刘国生,谢志雄,沈萍.细菌生物膜的结构及形成机制研究进展[J].氨基酸和生物资源,2006,28(3):30-33
[20]Nes IF,Yoon SS,Diep DB.Ribosomally synthesiszed antimicrobial peptides(bacteriocins)in lactic acid bacteria:a review[J].Food Science and Biotechnology,2007,16(5):675-690
[21]Cotter PD,Hill C,Ross RP.Bacteriocins:Developing innate immunity for food[J].Nature Reviews Microbiology,2005,3(10):777-788
[22]Stoll R,Goebel W.The major PEP-phosphotransferase systems(PTSs)for glucose mannose and cellobiose of Listeria monocytogenes,and their significance for extra-and intracellular growth[J].Microbiology,2010,156(4):1069-1083
[23]Kjos M,Nes IF,Diep DB.Mechanisms of resistance to bacteriocins targeting the mannose phosphotransferase system[J].Applied and Environmental Microbiology,2011,77(10):3335-3342