TTC法测定山豆根种子生活力条件的优化
|
摘要
以山豆根种子为实验材料,采用TTC法测定生活力,分别通过正交试验、单因素试验和Central Composite Design响应面试验对测定条件进行优化,从TTC浓度、染色温度和染色时间为指标。结果显示,各因素对种子生活力测定的影响差异不显著;单因素试验的最佳测试条件为TTC浓度0.7%、染色温度40℃、染色时间4 h;Central Composite Design响应面试验优化后,最佳测试条件与单因素试验结果一致。表明用响应面分析法对TTC法测定山豆根种子生活力的条件进行优化是可靠的。
In this paper, the seed vitality of the Sophora tonkinensis seeds was determined by TTC method. The measurement conditions were optimized through orthogonal test, single factor test and Central Composite Design response surface test, respectively, and the indexes of TTC concentration, dyeing temperature and dyeing time were also measured. The results showed that there was no significant difference in the effect of different factors on seed vigor. The optimal test conditions of single factor test were TTC concentration of 0.7%, dyeing temperature of 40 ℃ and dyeing time of 4 h, while the same results were also found in Central Composite Design response surface test after optimization. These results showed that the response surface analysis wer reliable to optimize the condition of TTC method for determining seed vigor in sophora tonkinensis.
In this paper, the seed vitality of the Sophora tonkinensis seeds was determined by TTC method. The measurement conditions were optimized through orthogonal test, single factor test and Central Composite Design response surface test, respectively, and the indexes of TTC concentration, dyeing temperature and dyeing time were also measured. The results showed that there was no significant difference in the effect of different factors on seed vigor. The optimal test conditions of single factor test were TTC concentration of 0.7%, dyeing temperature of 40 ℃ and dyeing time of 4 h, while the same results were also found in Central Composite Design response surface test after optimization. These results showed that the response surface analysis wer reliable to optimize the condition of TTC method for determining seed vigor in sophora tonkinensis.
引文
[1]《贵州植物志》编辑委员会.贵州植物志(第七卷)[M].成都:四川民族出版社,1988:370.
[2]国家药典委员会.中华人民共和国药典(第一部)[S].北京:中国医药科技出版社,2015:27.
[3]檀龙颜,马洪娜.越南槐繁殖与栽培技术的研究进展[J].种子,2017,36(08):52-56.
[4]成小璐,魏怡冰,魏升华,等.羊耳菊种子生活力的最佳测定条件[J].贵州农业科学,2016,44(8):100-104.
[5]王志威,魏升华,李俊,等.头花蓼种子生活力TTC法最优测定条件研究[J].南方农业,2018,12(25):1-6.
[2]国家药典委员会.中华人民共和国药典(第一部)[S].北京:中国医药科技出版社,2015:27.
[3]檀龙颜,马洪娜.越南槐繁殖与栽培技术的研究进展[J].种子,2017,36(08):52-56.
[4]成小璐,魏怡冰,魏升华,等.羊耳菊种子生活力的最佳测定条件[J].贵州农业科学,2016,44(8):100-104.
[5]王志威,魏升华,李俊,等.头花蓼种子生活力TTC法最优测定条件研究[J].南方农业,2018,12(25):1-6.