菌根真菌对芦苇铜吸收及抗铜能力的影响
|
摘要
接种菌根真菌作为重金属污染植物修复生物强化技术之一,已引起研究者的广泛关注。以芦苇(Phragmites australis)-菌根真菌共生系统为研究对象,研究水培条件下,0.02、1、5 mg·L~(-1) Cu胁迫条件下接种摩西管柄囊霉(Funneliformis mosseae)及根内球囊霉(Rhizophagus irregularis)对芦苇铜吸收及抗铜能力的影响。结果表明,芦苇茎叶及根系的生长发育均会受到铜胁迫的抑制,然而与茎叶相比,芦苇根系受到铜胁迫的影响更显著。在铜处理浓度为1 mg·L~(-1)和5 mg·L~(-1)时,与无菌剂组相比,摩西球囊霉接种组的芦苇根系中的SOD活性分别提高了26.25%、42.3%,而根内球囊霉接种组的SOD活性则呈现下降趋势。接种根内球囊霉使芦苇体内的铜浓度升高,在铜处理浓度为1 mg·L~(-1)时其茎叶及根系中的铜浓度比无菌剂组分别高出80.03%、33.6%,而在5 mg·L~(-1)时,则分别高出49.43%、8.53%,增幅较摩西球囊霉接种组显著。本研究证实了菌根真菌可以通过促进芦苇的生长及营养元素的吸收,降低Cu毒害,提高其对Cu的吸收效率,结果可为菌根真菌强化植物修复重金属污染环境提供新的参考。
The use of mycorrhizal technology as a bioaugmentation technique for phytoremediation of heavy metal contaminated soil has attracted wide attention of researchers. In this study, the symbiotic system of Phragmites australis mycorrhizal fungi was used as the core to analyze the stress-resistance response process and copper absorption characteristics of symbiotic systems under different copper stress conditions from the aspects of stress resistance and copper absorption efficiency of Phragmites australis. The results showed that the growth and development of stems, leaves and roots of Phragmites australis were inhibited by copper stress.However, compared with stems and leaves, the effects of copper stress on Phragmites australis roots were more significant. When the copper concentration was 1 mg·L~(-1) and 5 mg·L~(-1), the SOD activity in the roots of Phragmites australis inoculated with Funneliformis mosseae was increased by 26.25% and 42.3%, respectively, compared with the control group. The SOD activity of the Funneliformis mosseae inoculated group showed a downward trend. Inoculation of Rhizophagus irregularis increased the copper concentration in Phragmites australis. When the copper concentration was 1 mg·L~(-1), the copper concentration in the stems and leaves of Phragmites australis was higher than the control group by 80.03% and 33.6%, respectively. When the copper concentration was 5 mg·L~(-1), they were higher than those of the control group by 49.43% and 8.53%, respectively, and the degree of increase was significantly greater than that of the Funneliformis mosseae inoculated group. This study confirmes that mycorrhizal fungi can promote the growth and nutrient absorption of Phragmites australis, reduce Cu toxicity, increase its absorption efficiency of Cu, and provide a new reference for mycorrhizal fungi to strengthen phytoremediation of heavy metal pollution.
The use of mycorrhizal technology as a bioaugmentation technique for phytoremediation of heavy metal contaminated soil has attracted wide attention of researchers. In this study, the symbiotic system of Phragmites australis mycorrhizal fungi was used as the core to analyze the stress-resistance response process and copper absorption characteristics of symbiotic systems under different copper stress conditions from the aspects of stress resistance and copper absorption efficiency of Phragmites australis. The results showed that the growth and development of stems, leaves and roots of Phragmites australis were inhibited by copper stress.However, compared with stems and leaves, the effects of copper stress on Phragmites australis roots were more significant. When the copper concentration was 1 mg·L~(-1) and 5 mg·L~(-1), the SOD activity in the roots of Phragmites australis inoculated with Funneliformis mosseae was increased by 26.25% and 42.3%, respectively, compared with the control group. The SOD activity of the Funneliformis mosseae inoculated group showed a downward trend. Inoculation of Rhizophagus irregularis increased the copper concentration in Phragmites australis. When the copper concentration was 1 mg·L~(-1), the copper concentration in the stems and leaves of Phragmites australis was higher than the control group by 80.03% and 33.6%, respectively. When the copper concentration was 5 mg·L~(-1), they were higher than those of the control group by 49.43% and 8.53%, respectively, and the degree of increase was significantly greater than that of the Funneliformis mosseae inoculated group. This study confirmes that mycorrhizal fungi can promote the growth and nutrient absorption of Phragmites australis, reduce Cu toxicity, increase its absorption efficiency of Cu, and provide a new reference for mycorrhizal fungi to strengthen phytoremediation of heavy metal pollution.
引文
ALI N A,BERNAL MP,ATER M,2002.Tolerance and bioaccumulation of copper in Phragmites australis and Zea mays[J].Plant and Soil,239(1):103-111.
ALVARENGA P,GON?ALVES A P,FERNANDES R M,et al.,2008.Evaluation of composts and liming materials in the phytostabilization of a mine soil using perennial ryegrass[J].Science of The Total Environment,406(1-2):43-56.
ARIAS J A,PERALTA-VIDEA J R,ELLZEY J T,et al.,2010.Plant growth and metal distribution in tissues of Prosopis juliflora-velutina grown on chromium contaminated soil in the presence of Glomus deserticola[J].Environmental Science&Technology,44(19):7272-7279.
CARIAS C C,NOVAIS J M,MARTINS-DIAS S,2008.Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange[J].Bioresource Technology,99(2):243-251.
DADA E O,NJOKU K L,OSUNTOKI A A,et al.,2015.A review of current techniques for in situ physico-chemical and biological remediation of heavy metals polluted soil[J].Ethiopian Journal of Environmental Studies and Management,8(5):606-615.
DAS K,ROYCHOUDHURY A,2014.Reactive oxygen species(ROS)and response of antioxidants as ROS-scavengers during environmental stress in plants[J].Frontiers in Environmental Science,2(53):1-13
GARG N,BHANDARI P,2014.Cadmium toxicity in crop plants and its alleviation by arbuscular mycorrhizal(AM)fungi:An overview[J].Giornale Botanico Italiano,148(4):609-621.
GUO P,WANG T,LIU Y,et al.,2014.Phytostabilization potential of evening primrose(Oenothera glazioviana)for copper-contaminated sites[J].Environmental Science and Pollution Research,21(1):631-640.
HEADLEY T R,DAVISON L,HUETT D O,et al.,2012.Evapotranspiration from subsurface horizontal flow wetlands planted with Phragmites australis in sub-tropical Australia[J].Water Research,46(2):345-354.
HOAGLAND D R,ARNON D I,1950.The water culture method for growing plants without soil.California Agricultural Experimental Station Circular[M].Berkeley:University of California,347:1-32.
KALOGERAKIS N,VENIERI D,2016.Recent advances in bioremediation[J].Journal of Chemical Technology&Biotechnology,91(6):1575-1576.
KANWAL S,BANO A,MALIK R N,2015.Effects of Arbuscular Mycorrhizal Fungi on Metals Uptake,Physiological and Biochemical Response of Medicago Sativa L.with Increasing Zn and Cd Concentrations in Soil[J].American Journal of Plant Sciences,6(18):2906-2923.
KHAN A,SHARIF M,ALI A,et al.,2014.Potential of AM Fungi in phytoremediation of heavy metals and effect on yield of wheat crop[J].American Journal of Plant Sciences,5(11):1578-1586.
KORBOULEWSKY N,WANG R,BALDY V,2012.Purification processes involved in sludge treatment by a vertical flow wetland system:Focus on the role of the substrate and plants on N and P removal[J].Bioresource Technology,105:9-14.
LIU X H,SHEN Y X,LOU L Q,et al.,2009.Copper tolerance of the biomass crops Elephant grass(Pennisetum purpureum Schumach),Vetiver grass(Vetiveria zizanioides)and the upland reed(Phragmites australis)in soil culture[J].Biotechnology Advances,27(5):633-640.
MASSACCI A,PIETRINI F,IANNELLI M A,2001.Remediation of wetlands by Phragmites australis[J].Minerva Biotecnologica,13:135-140.
MEIER S,AZCóN R,CARTES P,et al.,2011.Alleviation of Cu toxicity in Oenothera picensis by copper-adapted arbuscular mycorrhizal fungi and treated agrowaste residue[J].Applied Soil Ecology,48(2):117-124.
MERLOS M A,ZITKA O,VOJTECH A,et al.,2016.The arbuscular mycorrhizal fungus Rhizophagus irregularis differentially regulates the copper response of two maize cultivars differing in copper tolerance[J].Plant Science,253:68-76.
PEREIRA R C,MONTERROSO C,MACíAS F,2010.Phytotoxicity of hexachlorocyclohexane:Effect on germination and early growth of different plant species[J].Chemosphere,79(3):326-333.
SMITH S E,SMITH F A,2011.Roles of arbuscular mycorrhizas in plant nutrition and growth:new paradigms from cellular to ecosystem scales[J].Annual Review of Plant Biology,62(1):227-250.
TAN S Y,JIANG Q Y,ZHUO F,et al.,2015.Effect of Inoculation with Glomus versiforme on Cadmium Accumulation,Antioxidant Activities and Phytochelatins of Solanum photeinocarpum[J].Plos One,10(7):0132347.
VAN DER ENT A,BAKER A J M,REEVES R D,et al.,2013.Hyperaccumulators of metal and metalloid trace elements:facts and fiction[J].Plant and Soil,362(1-2):319-334.
WANG L,HUANG X C,MA F,et al.,2017.Role of Rhizophagus irregularis,in alleviating cadmium toxicity via improving the growth,micro-and macroelements uptake in Phragmites australis[J].Environmental Science&Pollution Research International,24(4):3593-3607.
WILLIS A,RODRIGUES B F,HARRIS P J C,2013.The ecology of arbuscular mycorrhizal fungi[J].Critical Reviews in Plant Sciences,32:1-20.
WU S,ZHANG X,SUN Y,et al.,2015.Transformation and immobilization of chromium by arbuscular mycorrhizal fungi as revealed by SEM-EDS,TEM-EDS and XAFS[J].Environmental Science&Technology,49(24):14036-14047.
YE Z H,BAKER A J M,WONG M H,et al.,2003.Copper tolerance,uptake and accumulation by Phragmites australis[J].Chemosphere,50(6):795-800.
ZHANG X,REN B H,WU S L,et al.,2015.Arbuscular mycorrhizal symbiosis influences arsenic accumulation and speciation in Medicago truncatula L.in arsenic-contaminated soil[J].Chemosphere,119(1):224-230
ZHOU X S,FU L,XIA Y,et al.,2017.Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae[J].Metallomics,9:936-948.
陈雪,郑志鑫,石青,等,2017.AMF和植物富集土壤中铅和镉的效应[J].菌物研究,15(1):33-38.CHEN X,ZHENG Z X,SHI Q,et al.,2017.Effect of AMF and plants on accumulation of Pb and Cd in soil[J].Journal of Fungal Research,15(1):33-38.
董志成,鲍征宇,谢淑云,等,2008.湿地芦苇对有毒重金属元素的抗性及吸收和累积[J].地质科技情报,27(1):80-84.DONG Z C,BAO Z Y,XIE S Y,et al.,2008.Resistance and toxic heavy metal uptake and accumulation by phragmites australis in a wetland[J].Geological Science and Technology Information,27(1):80-84.
高丙,黄建,鲁彦君,2018.接种真菌对马尾松幼苗抗铜性的影响[J].西北林学院学报,33(2):146-150.GAO B,HUANG J,LU Y J,2018.On the impact of inoculating with ectomycorrhizal fungi on the copper resistance of Pinus massoniana seedlings[J].Journal of Northwest Forestry University,33(2):146-150.
林先贵,郝文英,1989.不同植物对VA菌根菌的依赖性[J].植物学报:英文版,31(9):721-725.LIN X G,HAO W Y,1989.Myccrrhizal dependency of various kinds of plants[J].Journal of Integrative Plant biology,31(9):721-725.
刘梦娇,张英伟,柴立伟,等,2017.铜胁迫下4种外生菌根真菌的耐受性比较[J].菌物研究,15(1):39-45.LIU M J,ZHANG Y W,CHAI L W,et al.,2017.Comparison the tolerance of four ectomycorrhizal fungi under copper stress[J].Journal of Fungal Research,15(1):39-45.
卢鑫,胡文友,黄标,等,2017.丛枝菌根真菌对玉米和续断菊间作镉吸收和累积的影响[J].土壤,49(1):111-117.LU X,HU W Y,HUANG B,et al.,2017.Effects of arbuscular mycorrhizal fungi(AMF)on Cd absorption and accumulation in maize and Sonchus asper L.hill using intercropping system[J].Soils,49(1):111-117.
孙丹萍,2004.丛枝菌根真菌扩繁技术研究[J].河南林业科技,24(2):12-13.SUN D P,2004.Study on propagation technology of arbuscular mycorrhizal fungi[J].Journal of Henan Forestry Science and Technology,24(2):12-13.
温祝桂,王杰,汤阳泽,等,2017.外生菌根真菌彩色豆马勃(Pisolithus tinctorius)辅助植物修复重金属Cu污染土壤的应用潜力[J].生物技术通报,33(4):149-156.WEN Z G,WANG J,TANG Y Z,et al.,2017.The application potential of ectomycorrhizal fungus Pisolithus tinctorius assisting plant in phytoremediation of Cu-contaminated soils[J].Biotechnology Bulletin,33(4):149-156.
肖家欣,安静,杨安娜,等,2011.五种丛枝菌根真菌对白三叶耐铜污染的影响[J].中国草地学报,33(6):57-63.XIAO J X,AN J,YANG A N,et al.,2011.Effects of five arbuscular mycorrhizal fungi on the tolerance of white clover(Trifolium repens)to copper contamination[J].Chinese Journal of Grassland,33(6):57-63.
杨秀敏,唐国忠,潘宇,等,2017.菌根对东南景天生长和吸收重金属的影响[J].金属矿山(12):163-168.YANG X M,TANG G Z,PAN Y,et al.,2017.Effects of arbuscular mycorrhizals on growth and heavy metal uptake of Sedum alfredii[J].Metal Mine(12):163-168.
尹宝海,史静,岳献荣,等,2017.外源磷与AMF菌丝对紫色土-玉米间作系统磷迁移的影响[J].生态环境学报,26(2):227-233.YIN B H,SHI J,YUE X R,et al.,2017.Effect of P Application and AMF hyphae on P transport in the purple soil-maize intercropping system[J].Ecology and Environment Sciences,26(2):227-233.
赵宁宁,邱丹,孟德凯,等,2017.AM真菌对蜈蚣草根围土壤砷形态及其砷吸收的影响[J].菌物学报,36(7):1048-1055.ZHAO N Y,QIU D,MENG D K,et al.,2017.Effects of arbuscular mycorrhizal fungi on arsenic fractionation in rhizosphere soil and arsenic accumulation by Pteris vittata[J].Mycosystema,36(7):1048-1055.
赵曦,黄艺,李娟,2015.外生菌根真菌Xerocomus chrysenteron产漆酶能力及其对外加DDT和重金属的响应[J].生态环境学报,24(2):329-335.ZHAO X,HUANG Y,LI J,2015.Efficiency of laccase secretion by the ectomycorrhizal fungus Xerocomus chrysenteron and its responses to the addition of DDT and heavy metals[J].Ecology and Environment Sciences,24(2):329-335.
ALVARENGA P,GON?ALVES A P,FERNANDES R M,et al.,2008.Evaluation of composts and liming materials in the phytostabilization of a mine soil using perennial ryegrass[J].Science of The Total Environment,406(1-2):43-56.
ARIAS J A,PERALTA-VIDEA J R,ELLZEY J T,et al.,2010.Plant growth and metal distribution in tissues of Prosopis juliflora-velutina grown on chromium contaminated soil in the presence of Glomus deserticola[J].Environmental Science&Technology,44(19):7272-7279.
CARIAS C C,NOVAIS J M,MARTINS-DIAS S,2008.Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange[J].Bioresource Technology,99(2):243-251.
DADA E O,NJOKU K L,OSUNTOKI A A,et al.,2015.A review of current techniques for in situ physico-chemical and biological remediation of heavy metals polluted soil[J].Ethiopian Journal of Environmental Studies and Management,8(5):606-615.
DAS K,ROYCHOUDHURY A,2014.Reactive oxygen species(ROS)and response of antioxidants as ROS-scavengers during environmental stress in plants[J].Frontiers in Environmental Science,2(53):1-13
GARG N,BHANDARI P,2014.Cadmium toxicity in crop plants and its alleviation by arbuscular mycorrhizal(AM)fungi:An overview[J].Giornale Botanico Italiano,148(4):609-621.
GUO P,WANG T,LIU Y,et al.,2014.Phytostabilization potential of evening primrose(Oenothera glazioviana)for copper-contaminated sites[J].Environmental Science and Pollution Research,21(1):631-640.
HEADLEY T R,DAVISON L,HUETT D O,et al.,2012.Evapotranspiration from subsurface horizontal flow wetlands planted with Phragmites australis in sub-tropical Australia[J].Water Research,46(2):345-354.
HOAGLAND D R,ARNON D I,1950.The water culture method for growing plants without soil.California Agricultural Experimental Station Circular[M].Berkeley:University of California,347:1-32.
KALOGERAKIS N,VENIERI D,2016.Recent advances in bioremediation[J].Journal of Chemical Technology&Biotechnology,91(6):1575-1576.
KANWAL S,BANO A,MALIK R N,2015.Effects of Arbuscular Mycorrhizal Fungi on Metals Uptake,Physiological and Biochemical Response of Medicago Sativa L.with Increasing Zn and Cd Concentrations in Soil[J].American Journal of Plant Sciences,6(18):2906-2923.
KHAN A,SHARIF M,ALI A,et al.,2014.Potential of AM Fungi in phytoremediation of heavy metals and effect on yield of wheat crop[J].American Journal of Plant Sciences,5(11):1578-1586.
KORBOULEWSKY N,WANG R,BALDY V,2012.Purification processes involved in sludge treatment by a vertical flow wetland system:Focus on the role of the substrate and plants on N and P removal[J].Bioresource Technology,105:9-14.
LIU X H,SHEN Y X,LOU L Q,et al.,2009.Copper tolerance of the biomass crops Elephant grass(Pennisetum purpureum Schumach),Vetiver grass(Vetiveria zizanioides)and the upland reed(Phragmites australis)in soil culture[J].Biotechnology Advances,27(5):633-640.
MASSACCI A,PIETRINI F,IANNELLI M A,2001.Remediation of wetlands by Phragmites australis[J].Minerva Biotecnologica,13:135-140.
MEIER S,AZCóN R,CARTES P,et al.,2011.Alleviation of Cu toxicity in Oenothera picensis by copper-adapted arbuscular mycorrhizal fungi and treated agrowaste residue[J].Applied Soil Ecology,48(2):117-124.
MERLOS M A,ZITKA O,VOJTECH A,et al.,2016.The arbuscular mycorrhizal fungus Rhizophagus irregularis differentially regulates the copper response of two maize cultivars differing in copper tolerance[J].Plant Science,253:68-76.
PEREIRA R C,MONTERROSO C,MACíAS F,2010.Phytotoxicity of hexachlorocyclohexane:Effect on germination and early growth of different plant species[J].Chemosphere,79(3):326-333.
SMITH S E,SMITH F A,2011.Roles of arbuscular mycorrhizas in plant nutrition and growth:new paradigms from cellular to ecosystem scales[J].Annual Review of Plant Biology,62(1):227-250.
TAN S Y,JIANG Q Y,ZHUO F,et al.,2015.Effect of Inoculation with Glomus versiforme on Cadmium Accumulation,Antioxidant Activities and Phytochelatins of Solanum photeinocarpum[J].Plos One,10(7):0132347.
VAN DER ENT A,BAKER A J M,REEVES R D,et al.,2013.Hyperaccumulators of metal and metalloid trace elements:facts and fiction[J].Plant and Soil,362(1-2):319-334.
WANG L,HUANG X C,MA F,et al.,2017.Role of Rhizophagus irregularis,in alleviating cadmium toxicity via improving the growth,micro-and macroelements uptake in Phragmites australis[J].Environmental Science&Pollution Research International,24(4):3593-3607.
WILLIS A,RODRIGUES B F,HARRIS P J C,2013.The ecology of arbuscular mycorrhizal fungi[J].Critical Reviews in Plant Sciences,32:1-20.
WU S,ZHANG X,SUN Y,et al.,2015.Transformation and immobilization of chromium by arbuscular mycorrhizal fungi as revealed by SEM-EDS,TEM-EDS and XAFS[J].Environmental Science&Technology,49(24):14036-14047.
YE Z H,BAKER A J M,WONG M H,et al.,2003.Copper tolerance,uptake and accumulation by Phragmites australis[J].Chemosphere,50(6):795-800.
ZHANG X,REN B H,WU S L,et al.,2015.Arbuscular mycorrhizal symbiosis influences arsenic accumulation and speciation in Medicago truncatula L.in arsenic-contaminated soil[J].Chemosphere,119(1):224-230
ZHOU X S,FU L,XIA Y,et al.,2017.Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae[J].Metallomics,9:936-948.
陈雪,郑志鑫,石青,等,2017.AMF和植物富集土壤中铅和镉的效应[J].菌物研究,15(1):33-38.CHEN X,ZHENG Z X,SHI Q,et al.,2017.Effect of AMF and plants on accumulation of Pb and Cd in soil[J].Journal of Fungal Research,15(1):33-38.
董志成,鲍征宇,谢淑云,等,2008.湿地芦苇对有毒重金属元素的抗性及吸收和累积[J].地质科技情报,27(1):80-84.DONG Z C,BAO Z Y,XIE S Y,et al.,2008.Resistance and toxic heavy metal uptake and accumulation by phragmites australis in a wetland[J].Geological Science and Technology Information,27(1):80-84.
高丙,黄建,鲁彦君,2018.接种真菌对马尾松幼苗抗铜性的影响[J].西北林学院学报,33(2):146-150.GAO B,HUANG J,LU Y J,2018.On the impact of inoculating with ectomycorrhizal fungi on the copper resistance of Pinus massoniana seedlings[J].Journal of Northwest Forestry University,33(2):146-150.
林先贵,郝文英,1989.不同植物对VA菌根菌的依赖性[J].植物学报:英文版,31(9):721-725.LIN X G,HAO W Y,1989.Myccrrhizal dependency of various kinds of plants[J].Journal of Integrative Plant biology,31(9):721-725.
刘梦娇,张英伟,柴立伟,等,2017.铜胁迫下4种外生菌根真菌的耐受性比较[J].菌物研究,15(1):39-45.LIU M J,ZHANG Y W,CHAI L W,et al.,2017.Comparison the tolerance of four ectomycorrhizal fungi under copper stress[J].Journal of Fungal Research,15(1):39-45.
卢鑫,胡文友,黄标,等,2017.丛枝菌根真菌对玉米和续断菊间作镉吸收和累积的影响[J].土壤,49(1):111-117.LU X,HU W Y,HUANG B,et al.,2017.Effects of arbuscular mycorrhizal fungi(AMF)on Cd absorption and accumulation in maize and Sonchus asper L.hill using intercropping system[J].Soils,49(1):111-117.
孙丹萍,2004.丛枝菌根真菌扩繁技术研究[J].河南林业科技,24(2):12-13.SUN D P,2004.Study on propagation technology of arbuscular mycorrhizal fungi[J].Journal of Henan Forestry Science and Technology,24(2):12-13.
温祝桂,王杰,汤阳泽,等,2017.外生菌根真菌彩色豆马勃(Pisolithus tinctorius)辅助植物修复重金属Cu污染土壤的应用潜力[J].生物技术通报,33(4):149-156.WEN Z G,WANG J,TANG Y Z,et al.,2017.The application potential of ectomycorrhizal fungus Pisolithus tinctorius assisting plant in phytoremediation of Cu-contaminated soils[J].Biotechnology Bulletin,33(4):149-156.
肖家欣,安静,杨安娜,等,2011.五种丛枝菌根真菌对白三叶耐铜污染的影响[J].中国草地学报,33(6):57-63.XIAO J X,AN J,YANG A N,et al.,2011.Effects of five arbuscular mycorrhizal fungi on the tolerance of white clover(Trifolium repens)to copper contamination[J].Chinese Journal of Grassland,33(6):57-63.
杨秀敏,唐国忠,潘宇,等,2017.菌根对东南景天生长和吸收重金属的影响[J].金属矿山(12):163-168.YANG X M,TANG G Z,PAN Y,et al.,2017.Effects of arbuscular mycorrhizals on growth and heavy metal uptake of Sedum alfredii[J].Metal Mine(12):163-168.
尹宝海,史静,岳献荣,等,2017.外源磷与AMF菌丝对紫色土-玉米间作系统磷迁移的影响[J].生态环境学报,26(2):227-233.YIN B H,SHI J,YUE X R,et al.,2017.Effect of P Application and AMF hyphae on P transport in the purple soil-maize intercropping system[J].Ecology and Environment Sciences,26(2):227-233.
赵宁宁,邱丹,孟德凯,等,2017.AM真菌对蜈蚣草根围土壤砷形态及其砷吸收的影响[J].菌物学报,36(7):1048-1055.ZHAO N Y,QIU D,MENG D K,et al.,2017.Effects of arbuscular mycorrhizal fungi on arsenic fractionation in rhizosphere soil and arsenic accumulation by Pteris vittata[J].Mycosystema,36(7):1048-1055.
赵曦,黄艺,李娟,2015.外生菌根真菌Xerocomus chrysenteron产漆酶能力及其对外加DDT和重金属的响应[J].生态环境学报,24(2):329-335.ZHAO X,HUANG Y,LI J,2015.Efficiency of laccase secretion by the ectomycorrhizal fungus Xerocomus chrysenteron and its responses to the addition of DDT and heavy metals[J].Ecology and Environment Sciences,24(2):329-335.