不同浓度比久对马铃薯组培苗生长的影响
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摘要
以马铃薯夏坡蒂组培苗为试验材料,设置6个浓度梯度比久加入MS培养基中,接种10d、20d、30d后,测定组培苗的苗高、茎粗、根数、根长、叶片数等指标。结果表明,随着比久浓度的增加,在培养10d、20d、30d时,组培苗的苗高均有所降低,且显著低于对照;组培苗的茎粗、根长和根条数均呈先升后降的趋势,且均在30mg·L-1处理达最大值;组培苗的叶片数均呈下降的趋势,且均在高于30mg·L-1处理时显著低于其他处理。可见,30mg·L-1的比久浓度对促进组培苗的生长最佳,可促进根部生长,降低株高,增加茎粗,进而培育壮苗,提升组培苗的质量。
In this experiment,the potato plantlets were used as materials.Six different concentrations of B9 were added to MS medium.To determine the seedling height,stem diameter,root numbers,root length and leaf numbers after 10 d,20d and 30 d.The results showed that the seedling height of plantlets was decreased after 10,20 and 30 days,and was significantly lower than control with the increasing of B9 concentrations.The stem diameter,root length and root numbers of plantlets were increased firstly and then decreased,and reached the maximum at 30mg·L-1 treatment.Leaf numbers of plantlets showed a downward trend,and were significantly lower than those of other treatments when they were higher than 30mg·L-1 treatment.Therefore,30mg·L-1 concentrations can promoting root growth and stem diameter,thus producing strong seedlings and improving the quality of plantlets.
In this experiment,the potato plantlets were used as materials.Six different concentrations of B9 were added to MS medium.To determine the seedling height,stem diameter,root numbers,root length and leaf numbers after 10 d,20d and 30 d.The results showed that the seedling height of plantlets was decreased after 10,20 and 30 days,and was significantly lower than control with the increasing of B9 concentrations.The stem diameter,root length and root numbers of plantlets were increased firstly and then decreased,and reached the maximum at 30mg·L-1 treatment.Leaf numbers of plantlets showed a downward trend,and were significantly lower than those of other treatments when they were higher than 30mg·L-1 treatment.Therefore,30mg·L-1 concentrations can promoting root growth and stem diameter,thus producing strong seedlings and improving the quality of plantlets.
引文
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[2]朱佳文,洪亚辉,萧浪涛,等.PP333和B9对白兰花生长的影响[J].湖南农业大学学报(自然科学版),2003,29(6):482-484.
[3]姜英,李志辉,彭彦,等.比久、多效唑、根太阳对金叶假连翘株型的控制效应[J].热带作物学报,2010,31(4):651-654.
[4]章志红,朱小强,姜如荣,等.比久和多效唑对矮牵牛生长和观赏品质的影响[J].北方园艺,2018(03):83-86.
[5]杨茹薇,孙梦瑄,秦勇,等.不同浓度多效唑和比久浸种对甜瓜幼苗质量的影响[J].北方园艺,2016(08):11-15.
[6]赵喜亭,邵换娟,李明军,等.植物生长延缓剂多效唑和比久对怀山药离体保存的影响[J].河南农业科学,2012,41(3):120-124.
[7]张小红.3种植物生长调节剂对马铃薯原原种植株徒长的调控作用[J].甘肃农业科技,2009,(06):36-38.
[8]郝文胜,赵永秀,杨丽华.比久在马铃薯良种繁育中的应用[J].中国种业,2004,(10):43-44.
[9]赵建萍,蒋小满,柏新富,等.PP333、B9、CCC对脱毒马铃薯试管苗繁殖的影响[J].植物生理通讯,2003,39(6):571-574.
[10]张小静,李鹏程,陈富,等.三种植物生长延缓剂对马铃薯试管苗生长和保存的影响[J].作物杂志,2012(05):117-120.
[11]朱云芬,程群,李卫东,等.植物生长延缓剂B9对马铃薯种质资源离体保存的影响[J].湖北农业科学,2014,53(15):3501-3503.