IBA处理对杂交榛根蘖育苗生根指标及相关氧化酶活性的影响
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摘要
以"龙榛1号"当年萌生条为试材,研究了不同IBA浓度处理(500、750、1 000、1 250mg·L~(-1))对平欧杂交榛根蘖育苗生根的影响,分析了根蘖育苗生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)活性的变化,并对外源激素处理与相关氧化酶活性与生根的关系进行了相关性分析,以期探讨其生根机理。结果表明:IBA浓度处理能显著地提高生根率,以1 000mg·L~(-1)的生根效果最佳,生根率和不定根质量均显著高于其它处理。生根过程中,POD和PPO活性呈先上升后下降趋势,POD活性在育苗后20d出现峰值;PPO活性在40d时达到峰值。生根率与POD、PPO活性呈极显著正相关。说明适宜浓度IBA能增加生根过程POD、PPO活性,从而提高生根率。
C.heterophylla×C.avellana(‘Longzhen No.1')seedlings were used as material.The effects of different IBA concentrations(500,750,1 000,1 250 mg·L-1)on the rooting of C.heterophylla × C.avellana(‘Longzhen No.1')seedlings were studied,and the dynamic changes of peroxidase(POD)and polyphenol oxidase(PPO)activities during rooting were analyzed.Correlation analysis was conducted in order to discuss the mechanism of rooting.The results showed that the IBA concentration treatment could significantly improve the rooting rate,and 1 000mg·L-1 had the best rooting effect.The rooting rate and adventitious root quality were significantly higher than those of other treatments.During rooting,the activities of POD and PPO increased first and then decreased,and the activity of POD peaked at 20 days after seedling cultivation,and the activity of PPO peaked at 40 days.Rooting rate was significantly positively correlated with POD and PPO activities.The use of suitable concentration of IBA could increase the activities of POD and PPO in rooting process and increase rooting rate.
C.heterophylla×C.avellana(‘Longzhen No.1')seedlings were used as material.The effects of different IBA concentrations(500,750,1 000,1 250 mg·L-1)on the rooting of C.heterophylla × C.avellana(‘Longzhen No.1')seedlings were studied,and the dynamic changes of peroxidase(POD)and polyphenol oxidase(PPO)activities during rooting were analyzed.Correlation analysis was conducted in order to discuss the mechanism of rooting.The results showed that the IBA concentration treatment could significantly improve the rooting rate,and 1 000mg·L-1 had the best rooting effect.The rooting rate and adventitious root quality were significantly higher than those of other treatments.During rooting,the activities of POD and PPO increased first and then decreased,and the activity of POD peaked at 20 days after seedling cultivation,and the activity of PPO peaked at 40 days.Rooting rate was significantly positively correlated with POD and PPO activities.The use of suitable concentration of IBA could increase the activities of POD and PPO in rooting process and increase rooting rate.
引文
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[16]王宏信,李向林,王楠,等.ABT1处理对降香黄檀硬枝扦插生根及其关联酶活性的影响[J].江苏农业科学,2017,45(8):138-141.
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[3]张洪.平欧杂种榛在北京地区的引种表现[J].中国果树,2010(6):40-43.
[4]车树理,刘玲玲,卢瑞林.榛子在甘肃定西的引种表现及栽培技术要点[J].山西果树,2009(5):20-21.
[5]万建华,陈炜青,姜成英,等.杂交榛子在甘肃的引种表现[J].甘肃林业科技,2010,35(1):74-76.
[6]邓贵义,张丹,张春燕.自然条件下平榛丰产栽培技术的研究[J].中国野生植物资源,2010(3):66-67.
[7]黄芳利,殷展波.杂交榛子早期丰产栽培技术[J].北方果树,2007(6):25.
[8]季长清,于薇薇,刘玉凤,等.杂交榛子丰产栽培技术[J].北方果树,2009(3):34.
[9]解明.杂交榛子丰产栽培技术(续Ⅰ)[J].北方果树,2011(5):54-57.
[10]扈红军,曹帮华,尹伟伦,等.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[11]宋丽红,曹帮华.光叶楮扦插生根的吲哚乙酸氧化酶、多酚氧化酶、过氧化物酶活性变化研究[J].武汉植物学研究,2005,23(4):347-350.
[12]曾晶珏,张成,李志辉,等.不同生根剂及处理枝条方式对钩栗扦插生根的影响[J].中南林业科技大学学报,2016,36(6):56-60.
[13]GASPAR T,KEVERS C,HAUSMAN J F,et al.Practical uses of peroxidase activity as a predictive maker of rooting performance of micropropagated shoots[J].Agronomie,1992,12(10):757-765.
[14]李明,黄卓烈,谭绍满.难易生根桉树过氧化物酶及其同工酶多型性比较研究[J].华南农业大学学报,2000,21(3):56-59.
[15]扈红军,曹帮华,尹伟伦,等.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[16]王宏信,李向林,王楠,等.ABT1处理对降香黄檀硬枝扦插生根及其关联酶活性的影响[J].江苏农业科学,2017,45(8):138-141.
[17]BASSUK N L,HUNTER L D,HOWARD B H.The apparent of polyphenol oxidase and phloridzin in the production of apple rooting cofactors[J].Joumal of Horticultural Science,1981,56(4):313-322.