铜绿假单胞菌生物膜对消毒剂抗性研究进展
摘要
<正>消化内镜根据Spaulding分类被归属为中度危险性器械~([1])。临床使用后的消化内镜必须经过规范清洗和高水平消毒后才能用于下一位患者~([2,3]),目前广泛使用的高水平消毒剂包括戊二醛、邻苯二甲醛、过氧乙酸、酸性氧化电位水和含氯消毒剂等。随着消毒剂的广泛使用,消毒剂抗性研究也开始被关注。早在20世纪50年代Chaplin发现细菌对消毒剂有类似抗药性的性质~([4]),之后Lowbury就提出铜绿假单胞菌对季铵盐类消毒剂有抗性~([5])。Chapman报道称细菌对氯已定、戊二醛、季铵盐及酚类化合物等部分消毒剂也产生了抗性~([6])。铜绿假单胞
引文
[1]中华人民共和国国家卫生和计划生育委员会.WST 367-2012医疗机构消毒技术规范[S].2012.
[2]中华医学会消化内镜分会清洗与消毒学组.中国消化内镜清洗消毒专家共识意见[J].中华消化内镜杂志,2014,31(11):617-623.
[3]Qiu L,Zhou Z,Liu Q,et al.Investigating the failure of repeated standard cleaning and disinfection of a Pseudomonas aeruginosainfected Pancreatic and biliary endoscope[J].Am J Infect Control,2015,43(8):e43-e46.
[4]Chaplin CE.Bacterial resistance to quaternary ammonium disinfectants[J].J Bacteriol,1952,63(4):453-458.
[5]顾春英,薛广波.细菌对消毒剂的抗性研究进展[J].中华医院感染学杂志,1997,7(4):62-66.
[6]Chapman JS.Characterizing bacterial resistance to preservatives and disinfectants[J].Int Biodeter Biodeger,1998,41(3-4):241-245.
[7]Sarah TS,Reno F,GNter K,et al.Emergence of Glutaraldehyde-Resistant Pseudomonas aeruginosa[J].Infect Cont Hosp Ep,2011,32(12):1173-1178.
[8]周冬梅,周岩岩,卢玉文.清洗环节质量控制对内镜管腔生物膜的影响[J].中国消毒学杂志,2018,35(2):159-160.
[9]Borlee BR,Goldman AD,Murakami K,et al.Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix[J].Mol Microbiol,2010,75(4):827-842.
[10]Herruzo-Cabrera R,Vizcaino-Alcaide MJ,Fernández-Aceero MJ.The influence of laboratory adaptation on test strains,such as Pseudomonas aeruginosa,in the evaluation of the antimicrobial efficacy of ortho-phthalaldehyde[J].J Hosp Infect,2004,57(3):217-222.
[11]Roberts CG.The role of biofilms in reprocessing medical devices[J].Am J Infect Control,2013,41(5Supp1):S77-S80.
[12]郑钱梅,韦红.胞外DNA在铜绿假单胞菌生物膜形成中的作用机制[J].儿科药学杂志,2018,24(6):51-54.
[13]Neves MS,Da SM,Ventura GM,et al.Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes[J].Gastrointest Endosc,2016,83(5):944-953.
[14]Jansen K,Wu M.Transmission of infection by flexible gastrointestinal endoscopy and bronchoscopy[J].Clin Microbiol Rev,2013,26(2):231-254.
[15]Kampf G,Fliss PM,Martiny H.Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?[J].World JGastrointest Endosc,2014,6(9):390-406.
[16]邓晶荣,牟凤林,邵林,等.多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析[J].甘肃医药,2017,36(7):562-564.
[17]李晓霞,郭阳,张瑞琴,等.铜绿假单胞菌耐药率与生物膜形成能力之间的相关性分析[J].中华医院感染学杂志,2014,24(12):2868-2870,2879.
[18]袁晨燕,韩勍,陈建明,等.铜绿假单胞菌细菌生物膜形成及耐药性分析[J].中华医院感染学杂志,2011,21(11):2161-2163.
[19]龚德钒.铜绿假单胞菌耐药性研究进展[J].临床医药文献电子杂志,2017,4(24):47-49.
[20]魏岚.3种常用消毒剂对院内感染常见病原菌效果研究[J].中国病原生物学杂志,2016,11(5):419-423.
[21]沈波,许琴芬,孙维敏,等.医院感染多药耐药菌对消毒剂抗性的分析[J].中华医院感染学杂志,2011,21(10):2048-2050.
[22]舒东娇,张丽娜,曹子晶,等.消毒后消化内镜中分离的铜绿假单胞菌对消毒剂的抗性分析[J].中国消毒学杂志,2015,32(9):948-950.
[23]Bridier A,Briandet R,Thomas V,et al.Comparative biocidal activity of peracetic acid,benzalkonium chloride and ortho-phthalaldehyde on77 bacterial strains[J].J Hosp Infect,2011,78(3):208-213.
[24]穆丽娟,王庆丰,张征,等.酶原液灌注与酶浸泡清洗内镜的效果比较[J].中国消毒学杂志,2009,26(5):554-555.
[25]栾耀芳,王冠,王婧男,等.多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析[J].检验医学与临床,2014,11(16):2207-2209.
[26]Kampf G,Ostermeyer C,Tschudin-Sutter S,et al.Resistance or adaptation?How susceptible is a‘glutaraldehyde-resistant’Pseudomonas aeruginosa isolate in the absence of selection pressure?[J].J Hosp Infect,2013,84(4):316-318.
[27]Vikram A,Bomberger JM,Bibby KJ.Efflux as a Glutaraldehyde Resistance Mechanism in Pseudomonas fluorescens and Pseudomonas aeruginosa Biofilms[J].Antimicnb Agents Ch,2015,59(6):3433-3440.
[28]高海娇,程古月,王玉莲,等.细菌主要外排泵及其调控蛋白研究进展[J].畜牧兽医学报,2017,48(11):2023-2033.
[29]孙影,顾觉奋.细菌外排泵抑制剂研究进展[J].药学进展,2018,42(6):411-419.
[30]魏兰芬,潘协商,钱文理,等.邻苯二甲醛用于内镜现场消毒效果观察[J].中国消毒学杂志,2014,31(6):562-564.
[31]宋向阳,李武平,马春丽,等.三种消毒剂对胃镜污染乙肝病毒去除效果的实验研究[J].中国消毒学杂志,2015,32(3):232-234,238.
[32]林丹.铜绿假单胞菌对邻苯二甲醛与碘伏抗性及其机制研究[D].福建福州:福建医科大学,2009.
[33]陆龙喜,陆烨,林军明,等.铜绿假单胞菌生物膜对不同消毒因子的抗力研究[J].中华医院感染学杂志,2011,21(9):1728-1731.
[34]Bradford BD,Seiberling KA,park FE,et al.Disinfection of rigid nasal endoscopes following in vitro contamination with Staphylococcus aureus,Streptococcus pneumoniae,Pseudomonas aeruginosa,and Haemophilus influenzae[J].JAMA Otolaryn Head Neck Surg,2013,139(6):574-578.
[35]Martín-Espada MC,D’Ors A,BartoloméMC,et al.Peracetic acid disinfectant efficacy against Pseudomonas aeruginosa,biofilms on polystyrene surfaces and comparison between methods to measure it[J].LWT-Food Sci Technol,2014,56(1):58-61.
[36]Chenjiao W,Hongyan Z,Qing G,et al.In-Use evaluation of peracetic acid for high-level disinfection of endoscopes[J].Gastroenterol Nurs,2016,39(2):116-120.
[37]Chino T,Nukui Y,Morishita Y,et al.Morphological bactericidal fast-acting effects of peracetic acid,a high-level disinfectant,against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing[J].Antimicrob Resist In,2017,6(1):122.
[38]Akinbobola A,Sherry L,Mckay WG,et al.Tolerance of Pseudomonas aeruginosa in in vitro biofilms to high level peracetic acid disinfection[J].J Hosp Infect,2017,97(2):162-168.
[39]方爱乔,席惠君,李兆申,等.次氯酸消毒液消毒消化内镜的临床应用效果评价[J].中华消化内镜杂志,2017,34(9):667-668.
[40]Nasr AM,Mostafa MS,Arnaout HH,et al.The effect of exposure to sub-inhibitory concentrations of hypochlorite and quaternary ammonium compounds on antimicrobial susceptibility of Pseudomonas aeruginosa[J].Am J Infect Control,2018,46(7):e57-e63.
[41]周凤莲,杨莉娜,余阳,等.常用消毒剂对临床分离多重耐药铜绿假单胞菌的杀灭效果[J].中国消毒学杂志,2010,27(3):250-252,256.
[42]Kóse H,Yapar N.The comparison of various disinfectants efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers[J].Turk J Med Sci,2017,47(4):1287-1294.
[43]邢玉斌,索继江,贾宁,等.细菌的消毒剂耐药性[J].微生物学通报,2006,33(3):184-188.
[44]Roberts CG.The role of biofilms in reprocessing medical devices[J].Am J Infect Control,2013,41(5):S77-S80.
[45]刘洁,王会平,董歧,等.北京某区医疗机构环境致病菌耐药性和消毒剂抗性研究[J].中国消毒学杂志,2016,33(9):850-852.
[2]中华医学会消化内镜分会清洗与消毒学组.中国消化内镜清洗消毒专家共识意见[J].中华消化内镜杂志,2014,31(11):617-623.
[3]Qiu L,Zhou Z,Liu Q,et al.Investigating the failure of repeated standard cleaning and disinfection of a Pseudomonas aeruginosainfected Pancreatic and biliary endoscope[J].Am J Infect Control,2015,43(8):e43-e46.
[4]Chaplin CE.Bacterial resistance to quaternary ammonium disinfectants[J].J Bacteriol,1952,63(4):453-458.
[5]顾春英,薛广波.细菌对消毒剂的抗性研究进展[J].中华医院感染学杂志,1997,7(4):62-66.
[6]Chapman JS.Characterizing bacterial resistance to preservatives and disinfectants[J].Int Biodeter Biodeger,1998,41(3-4):241-245.
[7]Sarah TS,Reno F,GNter K,et al.Emergence of Glutaraldehyde-Resistant Pseudomonas aeruginosa[J].Infect Cont Hosp Ep,2011,32(12):1173-1178.
[8]周冬梅,周岩岩,卢玉文.清洗环节质量控制对内镜管腔生物膜的影响[J].中国消毒学杂志,2018,35(2):159-160.
[9]Borlee BR,Goldman AD,Murakami K,et al.Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix[J].Mol Microbiol,2010,75(4):827-842.
[10]Herruzo-Cabrera R,Vizcaino-Alcaide MJ,Fernández-Aceero MJ.The influence of laboratory adaptation on test strains,such as Pseudomonas aeruginosa,in the evaluation of the antimicrobial efficacy of ortho-phthalaldehyde[J].J Hosp Infect,2004,57(3):217-222.
[11]Roberts CG.The role of biofilms in reprocessing medical devices[J].Am J Infect Control,2013,41(5Supp1):S77-S80.
[12]郑钱梅,韦红.胞外DNA在铜绿假单胞菌生物膜形成中的作用机制[J].儿科药学杂志,2018,24(6):51-54.
[13]Neves MS,Da SM,Ventura GM,et al.Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes[J].Gastrointest Endosc,2016,83(5):944-953.
[14]Jansen K,Wu M.Transmission of infection by flexible gastrointestinal endoscopy and bronchoscopy[J].Clin Microbiol Rev,2013,26(2):231-254.
[15]Kampf G,Fliss PM,Martiny H.Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?[J].World JGastrointest Endosc,2014,6(9):390-406.
[16]邓晶荣,牟凤林,邵林,等.多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析[J].甘肃医药,2017,36(7):562-564.
[17]李晓霞,郭阳,张瑞琴,等.铜绿假单胞菌耐药率与生物膜形成能力之间的相关性分析[J].中华医院感染学杂志,2014,24(12):2868-2870,2879.
[18]袁晨燕,韩勍,陈建明,等.铜绿假单胞菌细菌生物膜形成及耐药性分析[J].中华医院感染学杂志,2011,21(11):2161-2163.
[19]龚德钒.铜绿假单胞菌耐药性研究进展[J].临床医药文献电子杂志,2017,4(24):47-49.
[20]魏岚.3种常用消毒剂对院内感染常见病原菌效果研究[J].中国病原生物学杂志,2016,11(5):419-423.
[21]沈波,许琴芬,孙维敏,等.医院感染多药耐药菌对消毒剂抗性的分析[J].中华医院感染学杂志,2011,21(10):2048-2050.
[22]舒东娇,张丽娜,曹子晶,等.消毒后消化内镜中分离的铜绿假单胞菌对消毒剂的抗性分析[J].中国消毒学杂志,2015,32(9):948-950.
[23]Bridier A,Briandet R,Thomas V,et al.Comparative biocidal activity of peracetic acid,benzalkonium chloride and ortho-phthalaldehyde on77 bacterial strains[J].J Hosp Infect,2011,78(3):208-213.
[24]穆丽娟,王庆丰,张征,等.酶原液灌注与酶浸泡清洗内镜的效果比较[J].中国消毒学杂志,2009,26(5):554-555.
[25]栾耀芳,王冠,王婧男,等.多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析[J].检验医学与临床,2014,11(16):2207-2209.
[26]Kampf G,Ostermeyer C,Tschudin-Sutter S,et al.Resistance or adaptation?How susceptible is a‘glutaraldehyde-resistant’Pseudomonas aeruginosa isolate in the absence of selection pressure?[J].J Hosp Infect,2013,84(4):316-318.
[27]Vikram A,Bomberger JM,Bibby KJ.Efflux as a Glutaraldehyde Resistance Mechanism in Pseudomonas fluorescens and Pseudomonas aeruginosa Biofilms[J].Antimicnb Agents Ch,2015,59(6):3433-3440.
[28]高海娇,程古月,王玉莲,等.细菌主要外排泵及其调控蛋白研究进展[J].畜牧兽医学报,2017,48(11):2023-2033.
[29]孙影,顾觉奋.细菌外排泵抑制剂研究进展[J].药学进展,2018,42(6):411-419.
[30]魏兰芬,潘协商,钱文理,等.邻苯二甲醛用于内镜现场消毒效果观察[J].中国消毒学杂志,2014,31(6):562-564.
[31]宋向阳,李武平,马春丽,等.三种消毒剂对胃镜污染乙肝病毒去除效果的实验研究[J].中国消毒学杂志,2015,32(3):232-234,238.
[32]林丹.铜绿假单胞菌对邻苯二甲醛与碘伏抗性及其机制研究[D].福建福州:福建医科大学,2009.
[33]陆龙喜,陆烨,林军明,等.铜绿假单胞菌生物膜对不同消毒因子的抗力研究[J].中华医院感染学杂志,2011,21(9):1728-1731.
[34]Bradford BD,Seiberling KA,park FE,et al.Disinfection of rigid nasal endoscopes following in vitro contamination with Staphylococcus aureus,Streptococcus pneumoniae,Pseudomonas aeruginosa,and Haemophilus influenzae[J].JAMA Otolaryn Head Neck Surg,2013,139(6):574-578.
[35]Martín-Espada MC,D’Ors A,BartoloméMC,et al.Peracetic acid disinfectant efficacy against Pseudomonas aeruginosa,biofilms on polystyrene surfaces and comparison between methods to measure it[J].LWT-Food Sci Technol,2014,56(1):58-61.
[36]Chenjiao W,Hongyan Z,Qing G,et al.In-Use evaluation of peracetic acid for high-level disinfection of endoscopes[J].Gastroenterol Nurs,2016,39(2):116-120.
[37]Chino T,Nukui Y,Morishita Y,et al.Morphological bactericidal fast-acting effects of peracetic acid,a high-level disinfectant,against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing[J].Antimicrob Resist In,2017,6(1):122.
[38]Akinbobola A,Sherry L,Mckay WG,et al.Tolerance of Pseudomonas aeruginosa in in vitro biofilms to high level peracetic acid disinfection[J].J Hosp Infect,2017,97(2):162-168.
[39]方爱乔,席惠君,李兆申,等.次氯酸消毒液消毒消化内镜的临床应用效果评价[J].中华消化内镜杂志,2017,34(9):667-668.
[40]Nasr AM,Mostafa MS,Arnaout HH,et al.The effect of exposure to sub-inhibitory concentrations of hypochlorite and quaternary ammonium compounds on antimicrobial susceptibility of Pseudomonas aeruginosa[J].Am J Infect Control,2018,46(7):e57-e63.
[41]周凤莲,杨莉娜,余阳,等.常用消毒剂对临床分离多重耐药铜绿假单胞菌的杀灭效果[J].中国消毒学杂志,2010,27(3):250-252,256.
[42]Kóse H,Yapar N.The comparison of various disinfectants efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers[J].Turk J Med Sci,2017,47(4):1287-1294.
[43]邢玉斌,索继江,贾宁,等.细菌的消毒剂耐药性[J].微生物学通报,2006,33(3):184-188.
[44]Roberts CG.The role of biofilms in reprocessing medical devices[J].Am J Infect Control,2013,41(5):S77-S80.
[45]刘洁,王会平,董歧,等.北京某区医疗机构环境致病菌耐药性和消毒剂抗性研究[J].中国消毒学杂志,2016,33(9):850-852.