基因工程菌生产抗菌肽的研究进展
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摘要
目前,利用基因工程菌生产抗菌肽,提供有效和稳定的方法是抗菌肽生产的热点之一。了解抗菌肽工程菌表达系统、生产量尤为重要,为此本文阐述了抗菌肽的生产方式,介绍了基因工程菌表达系统的种类、组成及特点,分析了基因工程菌生产抗菌肽的主要困难因素,并对近些年利用基因工程菌生产抗菌肽的进展进行了综述。
Recently, it is the one of the hot topic for antimicrobial peptides(AMPs) to be produced using recombinant expression system with efficiency and stable protocols. It is particularly crucial for us to know different expression systems we use and their production. For this purpose, we narrate methods to produce AMPs, species, elements and properties of different expression systems, mainly there is an analysis for some difficult factors to construct engineered bacteria. And, we also reviewed the progress of producing antibacterial peptides using engineered bacteria in recent years.
Recently, it is the one of the hot topic for antimicrobial peptides(AMPs) to be produced using recombinant expression system with efficiency and stable protocols. It is particularly crucial for us to know different expression systems we use and their production. For this purpose, we narrate methods to produce AMPs, species, elements and properties of different expression systems, mainly there is an analysis for some difficult factors to construct engineered bacteria. And, we also reviewed the progress of producing antibacterial peptides using engineered bacteria in recent years.
引文
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[15]Ashcheulova DO,Efimova LV,Lushchyk AY,et al.Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli[J].Protein Expression and Purification,2018,143:38-44
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[17]Wu SQ,Zhao GJ,Wan J,et al.Optimization of expression conditions and purification of antibacterial peptide SMAP-29 in Escherichia coli[J].Jiangsu Agricultural Science,2018,46(9):44-47(in Chinese)吴三桥,赵冠杰,万健,等.抗菌肽SMAP-29在大肠杆菌中表达条件的优化及纯化研究[J].江苏农业科学,2018,46(9):44-47
[18]Alimu R,Mao XF,Liu ZY.Expression optimization and characterization of Tenebrio molitor antimicrobiol peptides TmAMP1m in Escherichia coli[J].Chinese Journal of Biotechnology,2013,29(6):836-847(in Chinese)热依汗古丽·阿里木,毛新芳,刘忠渊.黄粉虫抗菌肽在大肠杆菌中表达条件优化及活性分析[J].生物工程学报,2013,29(6):836-847
[19]Kuddus MR,Yamano M,Rumi F,et al.Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system[J].Biotechnology Progress,2017,33(6):1520-1528
[20]Hockney RC.Recent developments in heterologous protein production in Escherichia coli[J].Trends in Biotechnology,1994,12(11):456-463
[21]Luo JH,You ZL.Study on expression of heterologous gene in Pichiapastoris[J].Biotechnology Bulletin,2007(3):75-79(in Chinese)罗竞红,游自立.巴斯德毕赤酵母表达系统在外源基因表达中的研究进展[J].生物技术通报,2007(3):75-79
[22]Hu L,Wang XH.Comparison of expression and activity of anti-AFBIscFv in Escherichia coli and Pichia pastoris[J].Journal of Biology,2018(in Chinese)胡莉,王小红.抗黄曲霉毒素B1单链抗体在大肠杆菌和毕赤酵母中的表达和活性研究[J].生物学杂志,2018
[23]Huang QG,Liang L,Chen QH,et al.High-Level expression and antibacterial properties of antimicrobial peptide moricin in Pichia pastoris[J].Food Science,2017,38(16):36-42(in Chinese)黄钦耿,梁玲,陈巧红,等.小菜蛾moricin在毕赤酵母中的表达及抑菌活性分析[J].食品科学,2017,38(16):36-42
[24]Kuddus MR,Rumi F,Tsutsumi M,et al.Expression,purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris[J].Protein Expression and Purification,2016,122:15-22
[25]Shi W,Ma R,Zhou JY,et al.Expression and identification of Apidaecin in pichiapastoris[J].Journal of Oral Science Research,2017,33(5):471-474(in Chinese)施文,马瑞,周建业,等.Apidaecin型抗菌肽在毕赤酵母菌中的基因工程表达[J].口腔医学研究,2017,33(5):471-474
[26]Xu J.Construction of Bacillus subtilis strain engineered for expression of porcineβ-defensin-2/cecropin P1 antimicrobial peptides and its characterization[D].Qinhuangdao:Master’s Thesis of Yanshan University,2016(in Chinese)徐建.表达猪β防御素-2和cecropin P1抗菌肽枯草芽孢杆菌的构建及特性研究[D].秦皇岛:燕山大学硕士学位论文,2016
[27]Wei DD,Li XD,Shan AS.Expression of antibacterial peptide PR-FO in Bacillus subtilis[A]//Animal Nutrition Branch of Chinese Association of Animal Science and Veterinary Medicine proceedings of the Twelfth Animal Nutrition Symposium[C].Wuhan:Animal Nutrition Branch of Chinese Association of Animal Science and Veterinary Medicine,2016:697(in Chinese)魏丹丹,李晓丹,单安山.抗菌肽PR-FO在枯草芽孢杆菌中的表达[A]//中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集[C].武汉:中国畜牧兽医学会动物营养学分会,2016:697
[28]Wang N,Li G,Zou HQ,et al.High Expression ofporcineβ-defense in Bacillus subtilis[J].Southwest China Journal of Agricultural Sciences,2017,30(8):1910-1913(in Chinese)王娜,李钢,邹辉琴,等.猪β-防御素c DNA在枯草芽孢杆菌中的高效表达[J].西南农业学报,2017,30(8):1910-1913
[29]Jiang N,Zhang AZ,Zhang CX,et al.Expressing method of antimicrobial peptide CC31 in Bacillus subtilis:China,CN105238809A[P].2016-01-13(in Chinese)姜宁,张爱忠,张晨雪,等.抗菌肽CC31在枯草芽孢杆菌中的表达方法:中国,CN105238809A[P].2016-01-13
[30]He Q,Fu AY,Li TJ.Expression and one-step purification of the antimicrobial peptide cathelicidin-BF using the intein system in Bacillus subtilis[J].Journal of Industrial Microbiology&Biotechnology,2015,42(4):647-653
[31]Luan C,Zhang HW,Song DG,et al.Expressing antimicrobial peptide cathelicidin-BF in Bacillus subtilis using SUMOtechnology[J].Applied Microbiology and Biotechnology,2014,98(8):3651-3658
[32]Li P,Wen YA,Liu JB,et al.Secretory-expression of antimicrobial peptide bactenecin7 gene in Lactococcus lactis and analysis the bioactivity of its expression products[J].China Biotechnology,2009,29(1):70-74(in Chinese)李朴,文阳安,刘靳波,等.抗菌肽Bactenecin7重组质粒构建及其在乳酸菌的分泌表达和活性鉴定[J].中国生物工程杂志,2009,29(1):70-74
[33]Tan JS.Synthesis and secretory expression of the origined human and hybrid antimicrobial peptides from Lactococcus lactis[D].Guangzhou:Master’s Thesis of Southern Medical University,2015(in Chinese)谭嘉圣.人源及杂合抗菌肽在乳酸乳球菌中重组分泌表达[D].广州:南方医科大学硕士学位论文,2015
[2]Qi L,Jiang N,Zhang AZ,et al.Research present status and reform strategy of antimicrobialpeptides[J].China Animal Husbandry&VeterinaryMedicine,2016,43(2):450-456(in Chinese)祁丽,姜宁,张爱忠,等.抗菌肽研发现状及其改造策略[J].中国畜牧兽医,2016,43(2):450-456
[3]Abdelbaqi S,Deslouches B,Steckbeck J,et al.Novel engineered cationic antimicrobial peptides display broad-spectrum activity against Francisella tularensis,Yersinia pestis and Burkholderia pseudomallei[J].Journal of Medical Microbiology,2016,65(2):188-194
[4]Bandyopadhyay S,Lee M,Sivaraman J,et al.Model membrane interaction and DNA-binding of antimicrobial peptide lasioglossin II derived from bee venom[J].Biochemical and Biophysical Research Communications,2013,430(1):1-6
[5]Xia YH.Isolation,purification and activity analysis of antimicrobial peptide SSH19 from swine spleen[D].Xinxiang:Master’s Thesis of Henan University of Science and Technology,2017(in Chinese)夏一赫.猪脾脏抗菌肽SSH19的分离鉴定及其生物信息学分析[D].新乡:河南科技学院硕士学位论文,2017
[6]Zhang BQ.Isolation,purification and activity analysis of antimicrobial peptide BSN27 from bovine spleen[D].Xinxiang:Master’s Thesis of Henan University of Science and Technology,2017(in Chinese)张冰清.牛脾脏抗菌肽BSN27的分离、纯化及活性分析[D].新乡:河南科技学院硕士学位论文,2017.
[7]Park SI,Yoe SM.A novel cecropin-like peptide from black soldier fly,Hermetia illucens:isolation,structural and functional characterization[J].Entomological Research,2017,47(2):115-124
[8]Pei JJ,Jiang L.Antimicrobial peptide from mucus of Andrias davidianus:screening and purification by magnetic cell membrane separation technique[J].International Journal of Antimicrobial Agents,2017,50(1):41-46
[9]Wang XQ,Gao Y,Yin ZF,et al.Solid Phase synthesis and anti-microbial activities of the antibacterial peptide IB-367[J].Chinese Journal of Synthetic Chemistry,2016,24(7):600-603(in Chinese)王小青,高杨,尹志峰,等.抗菌肽IB-367的固相合成与抑菌活性[J].合成化学,2016,24(7):600-603
[10]Isaksson J,Brandsdal BO,Engqvist M,et al.A synthetic antimicrobial peptidomimetic(LTX 109):stereochemical impact on membrane disruption[J].Journal of Medicinal Chemistry,2011,54(16):5786-5795
[11]Shi YQ,Wang QQ,Wu LD,et al.Optimization of preparation of antimicrobial peptides by Two-Step enzymatic hydrolysis of fish scales using response surface methodology and antimicrobial activity of purified antimicrobial peptide[J].Food Science,2018,39(6):155-161(in Chinese)施永清,王巧巧,吴丹丽,等.响应面试验优化双酶酶解法制备鱼鳞抗菌肽工艺及其抑菌性能分析[J].食品科学,2018,39(6):155-161
[12]Esmaeilpour M,Ehsani MR,Aminlari M,et al.Antimicrobial peptides derived from goat’s milk whey proteins obtained by enzymatic hydrolysis[J].Journal of Food Biosciences and Technology,2017,7(1):65-72
[13]Parachin NS,Mulder KC,VianaAAB,et al.Expression systems for heterologous production of antimicrobial peptides[J].Peptides,2012,38(2):446-456
[14]Wanmakok M,Orrapin S,Intorasoot A,et al.Expression in Escherichia coli of novel recombinant hybrid antimicrobial peptide AL32-P113 with enhanced antimicrobial activity in vitro[J].Gene,2018,671:1-9
[15]Ashcheulova DO,Efimova LV,Lushchyk AY,et al.Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli[J].Protein Expression and Purification,2018,143:38-44
[16]He ZQ,Liu TJ,LüYJ,et al.Biological characteristics of the antibacterial peptide Fowlicidin-3 fusion expressed in Escherichia coli[J].Journal of Yangzhou University(Agricultural and Life Science Edition),2011,32(4):1-6(in Chinese)和祯泉,刘天健,吕英军,等.抗菌肽Folicidin-3在大肠杆菌中融合表达及抑菌活性[J].扬州大学学报:农业与生命科学版,2011,32(4):1-6
[17]Wu SQ,Zhao GJ,Wan J,et al.Optimization of expression conditions and purification of antibacterial peptide SMAP-29 in Escherichia coli[J].Jiangsu Agricultural Science,2018,46(9):44-47(in Chinese)吴三桥,赵冠杰,万健,等.抗菌肽SMAP-29在大肠杆菌中表达条件的优化及纯化研究[J].江苏农业科学,2018,46(9):44-47
[18]Alimu R,Mao XF,Liu ZY.Expression optimization and characterization of Tenebrio molitor antimicrobiol peptides TmAMP1m in Escherichia coli[J].Chinese Journal of Biotechnology,2013,29(6):836-847(in Chinese)热依汗古丽·阿里木,毛新芳,刘忠渊.黄粉虫抗菌肽在大肠杆菌中表达条件优化及活性分析[J].生物工程学报,2013,29(6):836-847
[19]Kuddus MR,Yamano M,Rumi F,et al.Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system[J].Biotechnology Progress,2017,33(6):1520-1528
[20]Hockney RC.Recent developments in heterologous protein production in Escherichia coli[J].Trends in Biotechnology,1994,12(11):456-463
[21]Luo JH,You ZL.Study on expression of heterologous gene in Pichiapastoris[J].Biotechnology Bulletin,2007(3):75-79(in Chinese)罗竞红,游自立.巴斯德毕赤酵母表达系统在外源基因表达中的研究进展[J].生物技术通报,2007(3):75-79
[22]Hu L,Wang XH.Comparison of expression and activity of anti-AFBIscFv in Escherichia coli and Pichia pastoris[J].Journal of Biology,2018(in Chinese)胡莉,王小红.抗黄曲霉毒素B1单链抗体在大肠杆菌和毕赤酵母中的表达和活性研究[J].生物学杂志,2018
[23]Huang QG,Liang L,Chen QH,et al.High-Level expression and antibacterial properties of antimicrobial peptide moricin in Pichia pastoris[J].Food Science,2017,38(16):36-42(in Chinese)黄钦耿,梁玲,陈巧红,等.小菜蛾moricin在毕赤酵母中的表达及抑菌活性分析[J].食品科学,2017,38(16):36-42
[24]Kuddus MR,Rumi F,Tsutsumi M,et al.Expression,purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris[J].Protein Expression and Purification,2016,122:15-22
[25]Shi W,Ma R,Zhou JY,et al.Expression and identification of Apidaecin in pichiapastoris[J].Journal of Oral Science Research,2017,33(5):471-474(in Chinese)施文,马瑞,周建业,等.Apidaecin型抗菌肽在毕赤酵母菌中的基因工程表达[J].口腔医学研究,2017,33(5):471-474
[26]Xu J.Construction of Bacillus subtilis strain engineered for expression of porcineβ-defensin-2/cecropin P1 antimicrobial peptides and its characterization[D].Qinhuangdao:Master’s Thesis of Yanshan University,2016(in Chinese)徐建.表达猪β防御素-2和cecropin P1抗菌肽枯草芽孢杆菌的构建及特性研究[D].秦皇岛:燕山大学硕士学位论文,2016
[27]Wei DD,Li XD,Shan AS.Expression of antibacterial peptide PR-FO in Bacillus subtilis[A]//Animal Nutrition Branch of Chinese Association of Animal Science and Veterinary Medicine proceedings of the Twelfth Animal Nutrition Symposium[C].Wuhan:Animal Nutrition Branch of Chinese Association of Animal Science and Veterinary Medicine,2016:697(in Chinese)魏丹丹,李晓丹,单安山.抗菌肽PR-FO在枯草芽孢杆菌中的表达[A]//中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集[C].武汉:中国畜牧兽医学会动物营养学分会,2016:697
[28]Wang N,Li G,Zou HQ,et al.High Expression ofporcineβ-defense in Bacillus subtilis[J].Southwest China Journal of Agricultural Sciences,2017,30(8):1910-1913(in Chinese)王娜,李钢,邹辉琴,等.猪β-防御素c DNA在枯草芽孢杆菌中的高效表达[J].西南农业学报,2017,30(8):1910-1913
[29]Jiang N,Zhang AZ,Zhang CX,et al.Expressing method of antimicrobial peptide CC31 in Bacillus subtilis:China,CN105238809A[P].2016-01-13(in Chinese)姜宁,张爱忠,张晨雪,等.抗菌肽CC31在枯草芽孢杆菌中的表达方法:中国,CN105238809A[P].2016-01-13
[30]He Q,Fu AY,Li TJ.Expression and one-step purification of the antimicrobial peptide cathelicidin-BF using the intein system in Bacillus subtilis[J].Journal of Industrial Microbiology&Biotechnology,2015,42(4):647-653
[31]Luan C,Zhang HW,Song DG,et al.Expressing antimicrobial peptide cathelicidin-BF in Bacillus subtilis using SUMOtechnology[J].Applied Microbiology and Biotechnology,2014,98(8):3651-3658
[32]Li P,Wen YA,Liu JB,et al.Secretory-expression of antimicrobial peptide bactenecin7 gene in Lactococcus lactis and analysis the bioactivity of its expression products[J].China Biotechnology,2009,29(1):70-74(in Chinese)李朴,文阳安,刘靳波,等.抗菌肽Bactenecin7重组质粒构建及其在乳酸菌的分泌表达和活性鉴定[J].中国生物工程杂志,2009,29(1):70-74
[33]Tan JS.Synthesis and secretory expression of the origined human and hybrid antimicrobial peptides from Lactococcus lactis[D].Guangzhou:Master’s Thesis of Southern Medical University,2015(in Chinese)谭嘉圣.人源及杂合抗菌肽在乳酸乳球菌中重组分泌表达[D].广州:南方医科大学硕士学位论文,2015