以Lorke法为基础的半致死剂量分析法在油酸和化香树果序及黄连素抗镉减毒评价中的应用
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摘要
镉污染严重威胁着农业的健康发展,如何有效抗镉减毒是保障农业生产安全的重要措施。然而,目前缺乏高效的抗镉药物及简便易行的评价方法。本试验借助Lorke法为基础的半致死剂量(LD_(50))分析方法 ,检测了油酸、化香树果序、黄连素等不同潜在的抗镉化合物的减毒作用。通过改良后的Lorke法测定不同抗镉药物对小鼠的经口镉LD_(50)的影响。结果表明,与200 mg/kg V_C相比,灌胃200 mg/kg油酸、化香树果序、黄连素能够减轻镉的毒性,提高镉的LD_(50)。LD_(50)由140～160 mg/kg分别上升至300～320 mg/kg(油酸组)、280～300 mg/kg(化香树果序组)和320～340 mg/kg(黄连素组)。以上结果表明,以Lorke法为基础的LD_(50)分析方法,能够评价出油酸、化香树果序、黄连素的减镉毒作用及其剂量-效应关系。以上结果同时证明了相同剂量下黄连素效果要优于油酸、优于化香树果序。
Cadmium pollution is a serious threat to the development of agriculture,control and reduction of cadmium is an important way to ensure the safe of agricultural production.However,effective methods for controlling and evaluating cadmium toxicity are not available currently.To effectively reduce the toxic and adverse effects caused by cadmium,the LD_(50) analysis method based on Lorke method was used to evaluate the attenuating effect of oleic acid,Platycarya strobilacea caryopsis and berberine on cadmium toxicity.The modified Lorke method was used to determine the effects of different anti-cadmium drugs on transoral cadmium LD_(50) in mice.The results showed that 200 mg/kg of oleic acid,P.strobilacea caryopsis and berberine could reduce the toxicity of cadmium and increase the LD_(50) of cadmium in mice compared with 200 mg/kg of vitamin C.Their LD_(50) of cadmium rose to300-320 mg/kg,280-300 mg/kg and 320-340 mg/kg,respectively.The results indicated that the LD_(50) analysis based on Lorke method was feasible to evaluate the attenuating effect of oleic acid,P.strobilacea caryopsis and berberine on cadmium toxicity,as well as the relationship between dose and effect.The results also proved that the attenuating effect of berberine on cadmium toxicity was better than that of oleic acid and P.strobilacea at the same dose.
Cadmium pollution is a serious threat to the development of agriculture,control and reduction of cadmium is an important way to ensure the safe of agricultural production.However,effective methods for controlling and evaluating cadmium toxicity are not available currently.To effectively reduce the toxic and adverse effects caused by cadmium,the LD_(50) analysis method based on Lorke method was used to evaluate the attenuating effect of oleic acid,Platycarya strobilacea caryopsis and berberine on cadmium toxicity.The modified Lorke method was used to determine the effects of different anti-cadmium drugs on transoral cadmium LD_(50) in mice.The results showed that 200 mg/kg of oleic acid,P.strobilacea caryopsis and berberine could reduce the toxicity of cadmium and increase the LD_(50) of cadmium in mice compared with 200 mg/kg of vitamin C.Their LD_(50) of cadmium rose to300-320 mg/kg,280-300 mg/kg and 320-340 mg/kg,respectively.The results indicated that the LD_(50) analysis based on Lorke method was feasible to evaluate the attenuating effect of oleic acid,P.strobilacea caryopsis and berberine on cadmium toxicity,as well as the relationship between dose and effect.The results also proved that the attenuating effect of berberine on cadmium toxicity was better than that of oleic acid and P.strobilacea at the same dose.
引文
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[13] WANG Jingwen,ZHANG Yuanyuan,FANG Zhijia,et al.Oleic acid alleviates cadmium-induced oxidative damage in rat by its radicals scavenging activity[J].Biological Trace Element Research,2018,18:1-6.
[14]陈美先.无机型离子交换树脂驱除几种中成药中超标铅镉汞砷的研究[D].郑州:河南大学,2016.
[15]陈易彬,张益,陈奎.化香树果序多糖抗氧化性[J].食品科技,2010,35(2):141-143.
[16] ZHANG Benjian,XU Dan,GUO Yu,et al.Protection by and anti-oxidant mechanism of berberine against rat liver fibrosis induced by multiple hepatotoxic factors[J].Clinical&Experimental Pharmacology&Physiology,2008,35(3):303-309.
[2] STAFFORD A D,ALAN S P,PARAMSOTHY J,et al.Soil cadmium and New Zealand dairy farms:impact of whole-farm contaminant variability on environmental management[J].Agriculture Ecosystems&Environment,2018,254:282-291.
[3] ZUGRAVU C A,PARVU M,PATRASCU D,et al.Cadmium pollution of some animal origin foods from Brasov and Dambovita County[J].Bulletin of University of Agricultural Sciences and Veterinary Medicine ClujNapoca.Agriculture,2008,65(2):453-458.
[4] ZHANG Runxiang,WANG Lisha,ZHAO Jianing,et al.Effects of selenium and cadmium on ion profiles in the brains of chickens[J].Biological Trace Element Research,2016,174(1):1-8.
[5] RAGHUVANSHI R,CHAUDHARI A,KUMAR G N.Amelioration of cadmium-and mercury-induced liver and Kidney damage in rats by genetically engineered probiotic Escherichia coli Nissle 1917 producing pyrroloquinoline quinone with oral supplementation of citric acid[J].Nutrition,2016,32(11/12):1285-1294.
[6] SHI L,CAO H,LUO J,et al.Effects of molybdenum and cadmium on the oxidative damage and kidney apoptosis in Duck[J].Ecotoxicol Environ Saf,2017,145:24-31.
[7]杨自军.镉的污染及对动物的危害与防治[J].中国动物保健,2008(5):55-60.
[8]陈爽,侯振中,管延杰,等.亚硒酸钠对小鼠的半数致死量测定[J].东北农业大学学报,2012,43(3):91-94.
[9]沈洁,谢海棠,孙瑞元.定量药理学:一个促进药物开发及转换型研究严谨思考的多学科领域[J].中国临床药理学与治疗学,2008,13(5):481-493.
[10] DIETRICH L.A new approach to practical acute toxicity testing[J].Archives of Toxicology,1983,54(4):275-287.
[11]王靖雯,陈泽娜,高健,等.油酸对小鼠急性镉中毒的缓解作用及镉残留的影响[J].生物学杂志,2018,8(24):1-6.
[12]刘莉莉,林岚,殷霄,等.镉毒性研究进展[J].中国职业医学,2012,39(5):445-447.
[13] WANG Jingwen,ZHANG Yuanyuan,FANG Zhijia,et al.Oleic acid alleviates cadmium-induced oxidative damage in rat by its radicals scavenging activity[J].Biological Trace Element Research,2018,18:1-6.
[14]陈美先.无机型离子交换树脂驱除几种中成药中超标铅镉汞砷的研究[D].郑州:河南大学,2016.
[15]陈易彬,张益,陈奎.化香树果序多糖抗氧化性[J].食品科技,2010,35(2):141-143.
[16] ZHANG Benjian,XU Dan,GUO Yu,et al.Protection by and anti-oxidant mechanism of berberine against rat liver fibrosis induced by multiple hepatotoxic factors[J].Clinical&Experimental Pharmacology&Physiology,2008,35(3):303-309.