不同浓度外源钙对梨果实石细胞的影响
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摘要
以田间多年生砀山酥梨果实及其诱导的愈伤组织为试验材料,通过施用不同浓度CaCl_2(0、0.25%、0.50%、0.75%),探究抑制石细胞形成的适宜钙浓度。结果表明,一定浓度的钙离子能够降低肉桂醇脱氢酶的活性,抑制木质素的合成,降低石细胞含量,且以0.50%CaCl_2效果最佳。不同种类及配比的激素对愈伤组织的诱导及继代培养效果不同,筛选出MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA+0.5 mg/L NAA为最适的砀山酥梨果实诱导培养基,MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L KT为最适的继代培养基。与田间材料和愈伤组织为研究对象的试验结果显示一致,由此推测外界因素对钙调控木质素合成的影响较小,适宜浓度的CaCl_2能够显著降低田间梨果实中的石细胞含量,提高食用品质。
引文
[1]Wei H,Liu L,Wang M D,et al.Differentially expressed genes related to the formation of russet fruit skin in a mutant of ‘Dangshansuli’ pear(Pyrus bretchnederi Rehd.)determined by suppression subtractive hybridization[J].Euphytica,2014,196(2):285-297.
[2]徐义流.砀山酥梨[M].北京:中国农业出版社,2009:1-3.
[3]李玲,蔡永萍,刘小阳.梨果实的石细胞[J].植物生理学通讯,2004,40(5):629-632.
[4]刘小阳,李玲,宗梅,等.梨果实石细胞含量分布及其对梨品质的影响[J].安徽农业大学学报,2004,31(1):104-106.
[5]王纪忠,陶书田,齐开杰,等.喷硼对梨果硼、钙含量及石细胞形成相关酶活性的影响[J].植物营养与肥料学报,2011,17(5):1250-1255.
[6]李疆,任莹莹,吐尔逊·阿依,等.氯化锌对库尔勒香梨果实品质的影响[J].经济林研究,2009,27(2):16-19.
[7]Palta J P.Role of Calcium in plant responses to stresses:linking basic research to the solution of practical problems[J].HortScience,1996,31(1):51-57.
[8]朱晓军,杨劲松,梁永超,等.盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J].中国农业科学,2004,37(10):1497-1503.
[9]孙大业,郭艳林,马力耕.细胞信号传导[M].北京:科学出版社,2001.
[10]王晓佳,贾永华,王春良.不同钙肥处理对金冠苹果品质的影响[J].安徽农业科学,2015,43(35):62-64.
[11]陈桂芬.施钙肥可提高春甜橘产量和品质[J].农家顾问,2014(2):31.
[12]文旭.不同有机钙肥对库尔勒香梨果实相关品质的影响研究[D].乌鲁木齐:新疆农业大学,2012.
[13]Raese J T,Drake S R,Staiff D C.Influence of different Calcium materials and spray timing on mineral composition,yield,fruit quality,and control of fruit disorders of ‘Anjou’ pears[J].Journal of Plant Nutrition,1995,18(4):823-838.
[14]李中勇,高东升.喷钙对设施油桃产量和品质的影响[J].落叶果树,2007,39(3):25-26.
[15]李丙智,梁俊,张林森,等.CA2000钙宝叶肥在富士苹果树上应用研究[J].西北林学院学报,2001,16(3):26-28.
[16]赵娟.梨果实愈伤组织对致腐真菌侵染的生理响应机制研究[D].杨凌:西北农林科技大学,2012.
[17]Syros T,Yupsanis T,Zafiriadis H,et al.Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretica L.[J].Journal of Plant Physiology,2004,161(1):69-77.
[18]Tao S T,Khanizadeh S,Zhang H,et al.Anatomy,ultrastructure and lignin distribution of stone cells in two Pyrus species[J].Plant Science,2009,176(3):413-419.
[19]Goffner D,Joffroy I,Grima-Pettenati J.purification and characterization of isoforms of cinnamyl alcohol dehydrogenase from eucalyptus,xylem[J].Planta,1992,188(1):48-53.
[20]陶书田.梨(Pyrus)果实石细胞的结构成分分析及相关酶基因的克隆[D].南京:南京农业大学,2009.
[21]Wang B,Zhang N,Yan C C,et al.Bagging for the development of stone cell and metabolism of lignin in Pyrus bretschneideri ‘Dangshan Suli’[J].Acta Horticulturae Sinica,2013,40(3):531-539.
[22]王丹阳.木质素代谢相关酶在梨石细胞合成中的作用[D].南京:南京农业大学,2014.
[23]Ma C,Zhang H P,Li J M,et al.Genome-wide analysis and characterization of molecular evolution of the HCT gene family in pear (Pyrus bretschneideri)[J].Plant Systematics and Evolution,2017,303(1):71-90.
[24]Cao Y P,Han Y H,Meng D D,et al.Structural,evolutionary,and functional analysis of the class Ⅲ peroxidase gene family in Chinese pear (Pyrus bretschneideri)[J].Frontiers in Plant Science,2016,7:1874.
[25]Lu G L,Li Z J,Zhang X F,et al.Expression analysis of lignin-associated genes in hard end pear (Pyrus pyrifolia Whangkeumbae) and its response to calcium chloride treatment conditions[J].Journal of Plant Growth Regulation,2015,34(2):251-262.
[26]Kim S J,Kim K W,Cho M H,et al.Expression of cinnamyl alcohol dehydrogenases and their putative homologues during Arabidopsis thaliana growth and development:lessons for database annotations[J].Phytochemistry,2007,68(14):1957-1974.
[2]徐义流.砀山酥梨[M].北京:中国农业出版社,2009:1-3.
[3]李玲,蔡永萍,刘小阳.梨果实的石细胞[J].植物生理学通讯,2004,40(5):629-632.
[4]刘小阳,李玲,宗梅,等.梨果实石细胞含量分布及其对梨品质的影响[J].安徽农业大学学报,2004,31(1):104-106.
[5]王纪忠,陶书田,齐开杰,等.喷硼对梨果硼、钙含量及石细胞形成相关酶活性的影响[J].植物营养与肥料学报,2011,17(5):1250-1255.
[6]李疆,任莹莹,吐尔逊·阿依,等.氯化锌对库尔勒香梨果实品质的影响[J].经济林研究,2009,27(2):16-19.
[7]Palta J P.Role of Calcium in plant responses to stresses:linking basic research to the solution of practical problems[J].HortScience,1996,31(1):51-57.
[8]朱晓军,杨劲松,梁永超,等.盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J].中国农业科学,2004,37(10):1497-1503.
[9]孙大业,郭艳林,马力耕.细胞信号传导[M].北京:科学出版社,2001.
[10]王晓佳,贾永华,王春良.不同钙肥处理对金冠苹果品质的影响[J].安徽农业科学,2015,43(35):62-64.
[11]陈桂芬.施钙肥可提高春甜橘产量和品质[J].农家顾问,2014(2):31.
[12]文旭.不同有机钙肥对库尔勒香梨果实相关品质的影响研究[D].乌鲁木齐:新疆农业大学,2012.
[13]Raese J T,Drake S R,Staiff D C.Influence of different Calcium materials and spray timing on mineral composition,yield,fruit quality,and control of fruit disorders of ‘Anjou’ pears[J].Journal of Plant Nutrition,1995,18(4):823-838.
[14]李中勇,高东升.喷钙对设施油桃产量和品质的影响[J].落叶果树,2007,39(3):25-26.
[15]李丙智,梁俊,张林森,等.CA2000钙宝叶肥在富士苹果树上应用研究[J].西北林学院学报,2001,16(3):26-28.
[16]赵娟.梨果实愈伤组织对致腐真菌侵染的生理响应机制研究[D].杨凌:西北农林科技大学,2012.
[17]Syros T,Yupsanis T,Zafiriadis H,et al.Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretica L.[J].Journal of Plant Physiology,2004,161(1):69-77.
[18]Tao S T,Khanizadeh S,Zhang H,et al.Anatomy,ultrastructure and lignin distribution of stone cells in two Pyrus species[J].Plant Science,2009,176(3):413-419.
[19]Goffner D,Joffroy I,Grima-Pettenati J.purification and characterization of isoforms of cinnamyl alcohol dehydrogenase from eucalyptus,xylem[J].Planta,1992,188(1):48-53.
[20]陶书田.梨(Pyrus)果实石细胞的结构成分分析及相关酶基因的克隆[D].南京:南京农业大学,2009.
[21]Wang B,Zhang N,Yan C C,et al.Bagging for the development of stone cell and metabolism of lignin in Pyrus bretschneideri ‘Dangshan Suli’[J].Acta Horticulturae Sinica,2013,40(3):531-539.
[22]王丹阳.木质素代谢相关酶在梨石细胞合成中的作用[D].南京:南京农业大学,2014.
[23]Ma C,Zhang H P,Li J M,et al.Genome-wide analysis and characterization of molecular evolution of the HCT gene family in pear (Pyrus bretschneideri)[J].Plant Systematics and Evolution,2017,303(1):71-90.
[24]Cao Y P,Han Y H,Meng D D,et al.Structural,evolutionary,and functional analysis of the class Ⅲ peroxidase gene family in Chinese pear (Pyrus bretschneideri)[J].Frontiers in Plant Science,2016,7:1874.
[25]Lu G L,Li Z J,Zhang X F,et al.Expression analysis of lignin-associated genes in hard end pear (Pyrus pyrifolia Whangkeumbae) and its response to calcium chloride treatment conditions[J].Journal of Plant Growth Regulation,2015,34(2):251-262.
[26]Kim S J,Kim K W,Cho M H,et al.Expression of cinnamyl alcohol dehydrogenases and their putative homologues during Arabidopsis thaliana growth and development:lessons for database annotations[J].Phytochemistry,2007,68(14):1957-1974.