摘要
目的建立一种甜高粱秆发酵渣中可溶性糖和乙醇的快速浸提方法。方法考察高速匀浆时间和后续是否超声振荡对甜高粱渣中可溶性糖和乙醇浸提效率的影响;采用扫描电镜观察甜高粱渣的形态,从细胞层面解释颗粒尺寸对浸提速度的影响;对浸提方法进行加标回收验证。结果浸提方法以高速匀浆3min为宜,在这个条件下,蔗糖和乙醇在低、中、高3个水平的加标回收率在90.17%~92.80%之间。结论该浸提方法可靠,操作简单,浸提时间短,可用于甜高粱秆发酵渣中可溶性糖和乙醇的快速浸提。
Objective To establish a rapid extraction method of soluble sugar and ethanol from sweet sorghum stalk bagasses. Methods The effects of high-speed homogenization time and subsequent ultrasonic on the extraction efficiency were investigated. The morphology of sweet sorghum stalk bagasses was observed by scanning electron microscopy, and the effect of particle size on the extraction efficiency was illustrated in the cellular level. The extraction method was verified by experiment of recovery. Results The results showed that the optimum extraction method was high speed homogenization for 3 min. Under this condition, the recoveries of sucrose and ethanol at low, medium and high spiked levels were ranged from 90.17% to 92.80%. Conclusion The method is reliable, simple and short in extraction time, which can be used for rapid extraction of soluble sugar and ethanol from sweet sorghum stalk bagasses.
引文
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